LOCUS       DAFCHQ010000001       120812 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000001 DAFCHQ010000000
VERSION     DAFCHQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 120812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 120812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..120812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.001"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(33..1184)
                     /locus_tag="LNC71_000001"
     CDS             complement(33..1184)
                     /locus_tag="LNC71_000001"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBK6465104.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1197..1634)
                     /locus_tag="LNC71_000002"
     CDS             complement(1197..1634)
                     /locus_tag="LNC71_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6465105.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1649..3415)
                     /locus_tag="LNC71_000003"
     CDS             complement(1649..3415)
                     /locus_tag="LNC71_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBK6465106.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3679..4356
                     /locus_tag="LNC71_000004"
     CDS             3679..4356
                     /locus_tag="LNC71_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465107.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4359..5486
                     /locus_tag="LNC71_000005"
     CDS             4359..5486
                     /locus_tag="LNC71_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465108.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5686..6126)
                     /locus_tag="LNC71_000006"
     CDS             complement(5686..6126)
                     /locus_tag="LNC71_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6465109.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6137..8170)
                     /locus_tag="LNC71_000007"
     CDS             complement(6137..8170)
                     /locus_tag="LNC71_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6465110.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8172..9875)
                     /locus_tag="LNC71_000008"
     CDS             complement(8172..9875)
                     /locus_tag="LNC71_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6465111.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9969..10919)
                     /gene="manA"
                     /locus_tag="LNC71_000009"
     CDS             complement(9969..10919)
                     /gene="manA"
                     /locus_tag="LNC71_000009"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBK6465112.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10958..11983)
                     /locus_tag="LNC71_000010"
     CDS             complement(10958..11983)
                     /locus_tag="LNC71_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK6465113.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            complement(12192..13061)
                     /locus_tag="LNC71_000011"
     CDS             complement(12192..13061)
                     /locus_tag="LNC71_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBK6465114.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13520..15412)
                     /locus_tag="LNC71_000012"
     CDS             complement(13520..15412)
                     /locus_tag="LNC71_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465115.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15409..17136)
                     /locus_tag="LNC71_000013"
     CDS             complement(15409..17136)
                     /locus_tag="LNC71_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465116.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17152..17802)
                     /locus_tag="LNC71_000014"
     CDS             complement(17152..17802)
                     /locus_tag="LNC71_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK6465117.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18006..18302
                     /locus_tag="LNC71_000015"
     CDS             18006..18302
                     /locus_tag="LNC71_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6465118.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18474..19094
                     /locus_tag="LNC71_000016"
     CDS             18474..19094
                     /locus_tag="LNC71_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBK6465119.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19186..20352)
                     /locus_tag="LNC71_000017"
     CDS             complement(19186..20352)
                     /locus_tag="LNC71_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6465120.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20364..20717)
                     /locus_tag="LNC71_000018"
     CDS             complement(20364..20717)
                     /locus_tag="LNC71_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK6465121.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20845..21867
                     /locus_tag="LNC71_000019"
     CDS             20845..21867
                     /locus_tag="LNC71_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBK6465122.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21886..22047
                     /locus_tag="LNC71_000020"
     CDS             21886..22047
                     /locus_tag="LNC71_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465123.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22306..22740)
                     /locus_tag="LNC71_000021"
     CDS             complement(22306..22740)
                     /locus_tag="LNC71_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465124.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22850..24709)
                     /locus_tag="LNC71_000022"
     CDS             complement(22850..24709)
                     /locus_tag="LNC71_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6465125.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24827..25987)
                     /locus_tag="LNC71_000023"
     CDS             complement(24827..25987)
                     /locus_tag="LNC71_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBK6465126.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26294..27718)
                     /gene="araA"
                     /locus_tag="LNC71_000024"
     CDS             complement(26294..27718)
                     /gene="araA"
                     /locus_tag="LNC71_000024"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBK6465127.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27734..28432)
                     /locus_tag="LNC71_000025"
     CDS             complement(27734..28432)
                     /locus_tag="LNC71_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK6465128.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28389..30050)
                     /locus_tag="LNC71_000026"
     CDS             complement(28389..30050)
                     /locus_tag="LNC71_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBK6465129.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30268..31668
                     /locus_tag="LNC71_000027"
     CDS             30268..31668
                     /locus_tag="LNC71_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBK6465130.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31920..32999)
                     /locus_tag="LNC71_000028"
     CDS             complement(31920..32999)
                     /locus_tag="LNC71_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6465131.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33085..34044)
                     /locus_tag="LNC71_000029"
     CDS             complement(33085..34044)
                     /locus_tag="LNC71_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK6465132.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34056..35567)
                     /locus_tag="LNC71_000030"
     CDS             complement(34056..35567)
                     /locus_tag="LNC71_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK6465133.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35589..36152)
                     /locus_tag="LNC71_000031"
     CDS             complement(35589..36152)
                     /locus_tag="LNC71_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBK6465134.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36158..37021)
                     /locus_tag="LNC71_000032"
     CDS             complement(36158..37021)
                     /locus_tag="LNC71_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6465135.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37035..37931)
                     /locus_tag="LNC71_000033"
     CDS             complement(37035..37931)
                     /locus_tag="LNC71_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6465136.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38106..39395)
                     /locus_tag="LNC71_000034"
     CDS             complement(38106..39395)
                     /locus_tag="LNC71_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6465137.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39367..40884)
                     /locus_tag="LNC71_000035"
     CDS             complement(39367..40884)
                     /locus_tag="LNC71_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBK6465138.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41082..41972
                     /locus_tag="LNC71_000036"
     CDS             41082..41972
                     /locus_tag="LNC71_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBK6465139.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42045..42869)
                     /gene="nadE"
                     /locus_tag="LNC71_000037"
     CDS             complement(42045..42869)
                     /gene="nadE"
                     /locus_tag="LNC71_000037"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBK6465140.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42881..44353)
                     /locus_tag="LNC71_000038"
     CDS             complement(42881..44353)
                     /locus_tag="LNC71_000038"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBK6465141.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44990..45352)
                     /locus_tag="LNC71_000039"
     CDS             complement(44990..45352)
                     /locus_tag="LNC71_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK6465142.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45575..46327
                     /locus_tag="LNC71_000040"
     CDS             45575..46327
                     /locus_tag="LNC71_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6465143.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46467..47390)
                     /locus_tag="LNC71_000041"
     CDS             complement(46467..47390)
                     /locus_tag="LNC71_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6465144.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47405..47677)
                     /locus_tag="LNC71_000042"
     CDS             complement(47405..47677)
                     /locus_tag="LNC71_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465145.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(48147..49772)
                     /gene="groL"
                     /locus_tag="LNC71_000043"
     CDS             complement(48147..49772)
                     /gene="groL"
                     /locus_tag="LNC71_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBK6465146.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(49823..50107)
                     /gene="groES"
                     /locus_tag="LNC71_000044"
     CDS             complement(49823..50107)
                     /gene="groES"
                     /locus_tag="LNC71_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBK6465147.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            50332..50994
                     /locus_tag="LNC71_000045"
     CDS             50332..50994
                     /locus_tag="LNC71_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6465148.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(51070..52362)
                     /locus_tag="LNC71_000046"
     CDS             complement(51070..52362)
                     /locus_tag="LNC71_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6465149.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            52532..53161
                     /locus_tag="LNC71_000047"
     CDS             52532..53161
                     /locus_tag="LNC71_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBK6465150.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(53264..54073)
                     /locus_tag="LNC71_000048"
     CDS             complement(53264..54073)
                     /locus_tag="LNC71_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6465151.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(54128..54997)
                     /locus_tag="LNC71_000049"
     CDS             complement(54128..54997)
                     /locus_tag="LNC71_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBK6465152.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(54998..56314)
                     /locus_tag="LNC71_000050"
     CDS             complement(54998..56314)
                     /locus_tag="LNC71_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465153.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(56311..58146)
                     /gene="walK"
                     /locus_tag="LNC71_000051"
     CDS             complement(56311..58146)
                     /gene="walK"
                     /locus_tag="LNC71_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBK6465154.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(58151..58855)
                     /locus_tag="LNC71_000052"
     CDS             complement(58151..58855)
                     /locus_tag="LNC71_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6465155.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(59045..59905)
                     /locus_tag="LNC71_000053"
     CDS             complement(59045..59905)
                     /locus_tag="LNC71_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6465156.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(59892..60461)
                     /locus_tag="LNC71_000054"
     CDS             complement(59892..60461)
                     /locus_tag="LNC71_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBK6465157.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            60842..60914
                     /locus_tag="LNC71_000055"
     tRNA            60842..60914
                     /locus_tag="LNC71_000055"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60875..60877,aa:Lys,seq:ctt)
     gene            complement(61410..61796)
                     /locus_tag="LNC71_000056"
     CDS             complement(61410..61796)
                     /locus_tag="LNC71_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK6465158.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(61793..62314)
                     /locus_tag="LNC71_000057"
     CDS             complement(61793..62314)
                     /locus_tag="LNC71_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK6465159.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(62316..63350)
                     /gene="sppA"
                     /locus_tag="LNC71_000058"
     CDS             complement(62316..63350)
                     /gene="sppA"
                     /locus_tag="LNC71_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK6465160.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(63383..64798)
                     /locus_tag="LNC71_000059"
     CDS             complement(63383..64798)
                     /locus_tag="LNC71_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBK6465161.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(64767..66920)
                     /locus_tag="LNC71_000060"
     CDS             complement(64767..66920)
                     /locus_tag="LNC71_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBK6465162.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            67178..67414
                     /locus_tag="LNC71_000061"
     CDS             67178..67414
                     /locus_tag="LNC71_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBK6465163.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            67433..67648
                     /locus_tag="LNC71_000062"
     CDS             67433..67648
                     /locus_tag="LNC71_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HBK6465164.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(68351..69103)
                     /locus_tag="LNC71_000063"
     CDS             complement(68351..69103)
                     /locus_tag="LNC71_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6465165.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(69118..70365)
                     /locus_tag="LNC71_000064"
     CDS             complement(69118..70365)
                     /locus_tag="LNC71_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6465166.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(70441..70587)
                     /locus_tag="LNC71_000065"
     CDS             complement(70441..70587)
                     /locus_tag="LNC71_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBK6465167.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(70691..71002)
                     /locus_tag="LNC71_000066"
     CDS             complement(70691..71002)
                     /locus_tag="LNC71_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6465168.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(71004..71201)
                     /locus_tag="LNC71_000067"
     CDS             complement(71004..71201)
                     /locus_tag="LNC71_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBK6465169.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(71595..73322)
                     /locus_tag="LNC71_000068"
     CDS             complement(71595..73322)
                     /locus_tag="LNC71_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBK6465170.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(73315..74508)
                     /locus_tag="LNC71_000069"
     CDS             complement(73315..74508)
                     /locus_tag="LNC71_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6465171.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(74695..75339)
                     /locus_tag="LNC71_000070"
     CDS             complement(74695..75339)
                     /locus_tag="LNC71_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6465172.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(75433..75567)
                     /locus_tag="LNC71_000071"
     CDS             complement(75433..75567)
                     /locus_tag="LNC71_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBK6465173.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(75569..77701)
                     /gene="feoB"
                     /locus_tag="LNC71_000072"
     CDS             complement(75569..77701)
                     /gene="feoB"
                     /locus_tag="LNC71_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBK6465174.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(77698..78171)
                     /locus_tag="LNC71_000073"
     CDS             complement(77698..78171)
                     /locus_tag="LNC71_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBK6465175.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            78572..78796
                     /gene="nrdH"
                     /locus_tag="LNC71_000074"
     CDS             78572..78796
                     /gene="nrdH"
                     /locus_tag="LNC71_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBK6465176.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            78955..81114
                     /gene="nrdE"
                     /locus_tag="LNC71_000075"
     CDS             78955..81114
                     /gene="nrdE"
                     /locus_tag="LNC71_000075"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBK6465177.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            81164..82129
                     /gene="nrdF"
                     /locus_tag="LNC71_000076"
     CDS             81164..82129
                     /gene="nrdF"
                     /locus_tag="LNC71_000076"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBK6465178.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(82218..83357)
                     /locus_tag="LNC71_000077"
     CDS             complement(82218..83357)
                     /locus_tag="LNC71_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6465179.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(83666..84379)
                     /locus_tag="LNC71_000078"
     CDS             complement(83666..84379)
                     /locus_tag="LNC71_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBK6465180.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(84633..85334)
                     /gene="nagB"
                     /locus_tag="LNC71_000079"
     CDS             complement(84633..85334)
                     /gene="nagB"
                     /locus_tag="LNC71_000079"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBK6465181.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            85568..87100
                     /locus_tag="LNC71_000080"
     CDS             85568..87100
                     /locus_tag="LNC71_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6465182.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(87291..88493)
                     /locus_tag="LNC71_000081"
     CDS             complement(87291..88493)
                     /locus_tag="LNC71_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6465183.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(88575..89264)
                     /locus_tag="LNC71_000082"
     CDS             complement(88575..89264)
                     /locus_tag="LNC71_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6465184.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(89370..89738)
                     /gene="rplL"
                     /locus_tag="LNC71_000083"
     CDS             complement(89370..89738)
                     /gene="rplL"
                     /locus_tag="LNC71_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBK6465185.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(89791..90294)
                     /locus_tag="LNC71_000084"
     CDS             complement(89791..90294)
                     /locus_tag="LNC71_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBK6465186.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(90498..90625)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(90704..91393)
                     /gene="rplA"
                     /locus_tag="LNC71_000085"
     CDS             complement(90704..91393)
                     /gene="rplA"
                     /locus_tag="LNC71_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBK6465187.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(91497..91919)
                     /gene="rplK"
                     /locus_tag="LNC71_000086"
     CDS             complement(91497..91919)
                     /gene="rplK"
                     /locus_tag="LNC71_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBK6465188.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            92262..92930
                     /gene="sdaAB"
                     /locus_tag="LNC71_000087"
     CDS             92262..92930
                     /gene="sdaAB"
                     /locus_tag="LNC71_000087"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBK6465189.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            92943..93815
                     /gene="sdaAA"
                     /locus_tag="LNC71_000088"
     CDS             92943..93815
                     /gene="sdaAA"
                     /locus_tag="LNC71_000088"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBK6465190.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(93906..94925)
                     /locus_tag="LNC71_000089"
     CDS             complement(93906..94925)
                     /locus_tag="LNC71_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK6465191.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKY
                     TKLMD"
     gene            complement(95098..96003)
                     /locus_tag="LNC71_000090"
     CDS             complement(95098..96003)
                     /locus_tag="LNC71_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6465192.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(96062..96607)
                     /gene="nusG"
                     /locus_tag="LNC71_000091"
     CDS             complement(96062..96607)
                     /gene="nusG"
                     /locus_tag="LNC71_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBK6465193.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(96708..96878)
                     /gene="secE"
                     /locus_tag="LNC71_000092"
     CDS             complement(96708..96878)
                     /gene="secE"
                     /locus_tag="LNC71_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBK6465194.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(96902..97054)
                     /gene="rpmG"
                     /locus_tag="LNC71_000093"
     CDS             complement(96902..97054)
                     /gene="rpmG"
                     /locus_tag="LNC71_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK6465195.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            97216..97860
                     /locus_tag="LNC71_000094"
     CDS             97216..97860
                     /locus_tag="LNC71_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6465196.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(97916..98419)
                     /locus_tag="LNC71_000095"
     CDS             complement(97916..98419)
                     /locus_tag="LNC71_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK6465197.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(98432..98478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            99016..99981
                     /locus_tag="LNC71_000096"
     CDS             99016..99981
                     /locus_tag="LNC71_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK6465198.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(100080..101252)
                     /locus_tag="LNC71_000097"
     CDS             complement(100080..101252)
                     /locus_tag="LNC71_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465199.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(101239..102384)
                     /locus_tag="LNC71_000098"
     CDS             complement(101239..102384)
                     /locus_tag="LNC71_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465200.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(102398..103322)
                     /locus_tag="LNC71_000099"
                     /pseudo
     CDS             complement(102398..103322)
                     /locus_tag="LNC71_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            103439..104518
                     /locus_tag="LNC71_000100"
     CDS             103439..104518
                     /locus_tag="LNC71_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6465201.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            104550..105143
                     /locus_tag="LNC71_000101"
     CDS             104550..105143
                     /locus_tag="LNC71_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6465202.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(105253..105480)
                     /locus_tag="LNC71_000102"
     CDS             complement(105253..105480)
                     /locus_tag="LNC71_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6465203.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(105583..108171)
                     /locus_tag="LNC71_000103"
     CDS             complement(105583..108171)
                     /locus_tag="LNC71_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBK6465204.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(108191..108850)
                     /locus_tag="LNC71_000104"
     CDS             complement(108191..108850)
                     /locus_tag="LNC71_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6465205.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(108899..109402)
                     /gene="trmL"
                     /locus_tag="LNC71_000105"
     CDS             complement(108899..109402)
                     /gene="trmL"
                     /locus_tag="LNC71_000105"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBK6465206.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(109623..110489)
                     /locus_tag="LNC71_000106"
     CDS             complement(109623..110489)
                     /locus_tag="LNC71_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6465207.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            110598..111239
                     /locus_tag="LNC71_000107"
     CDS             110598..111239
                     /locus_tag="LNC71_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBK6465208.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(111301..112662)
                     /gene="mgtE"
                     /locus_tag="LNC71_000108"
     CDS             complement(111301..112662)
                     /gene="mgtE"
                     /locus_tag="LNC71_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBK6465209.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(112680..113579)
                     /locus_tag="LNC71_000109"
     CDS             complement(112680..113579)
                     /locus_tag="LNC71_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6465210.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(113581..114378)
                     /locus_tag="LNC71_000110"
     CDS             complement(113581..114378)
                     /locus_tag="LNC71_000110"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBK6465211.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(114356..115060)
                     /locus_tag="LNC71_000111"
     CDS             complement(114356..115060)
                     /locus_tag="LNC71_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBK6465212.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            115191..115767
                     /locus_tag="LNC71_000112"
                     /pseudo
     CDS             115191..115767
                     /locus_tag="LNC71_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            115885..116562
                     /locus_tag="LNC71_000113"
     CDS             115885..116562
                     /locus_tag="LNC71_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK6465213.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(116641..118449)
                     /gene="pepF"
                     /locus_tag="LNC71_000114"
     CDS             complement(116641..118449)
                     /gene="pepF"
                     /locus_tag="LNC71_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBK6465214.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(118625..119623)
                     /locus_tag="LNC71_000115"
     CDS             complement(118625..119623)
                     /locus_tag="LNC71_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBK6465215.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(119849..120502)
                     /locus_tag="LNC71_000116"
     CDS             complement(119849..120502)
                     /locus_tag="LNC71_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBK6465216.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        35800 a        24990 c        20657 g        39365 t
ORIGIN      
        1 tttcgtcaca ttcccctttt tcgtatccta ttttaagcga cttgcttggc ataacggttc
       61 acgttttgct tgatattccc gattgttttc ggatcattga ttcctgtgac ttcttgaatc
      121 aattgttcca agttttcatg gatcttcatt tcatgcaatt gacggctttg ttcatcttca
      181 ggatcatagt aattaaaaat aatccccatt gttgccgtca aatgtgggca agtcaagtta
      241 tgttcttgta gttctctcac tggacgtgta aatctttctt gataacctaa cttacgtaat
      301 ggtgtccgag cgacacgtgt gatcgcatca gagatatttt tgttttggaa gcgatgaatg
      361 attttttcaa tatacgcatg atgctgctct gcatcaaacc cccatttggc aatcagcaaa
      421 cttcctgttt cctgcaaaac tgattttaat tgtgccacaa ctaacgcatc ttgcattgct
      481 tcatcaatgg tctggtaacc taacaaggca cctgtataag cgacagtcgc atgaccagta
      541 ttgacactaa atagtttgcg ttcgatatat ggttctagat catctacata taaaacccct
      601 tttaattgga tttttttatt ttttctttgg ctatcgtcaa tgacccattc tttgaaaggt
      661 tccacttgga cgaataaagg atcttcatgg tgttgtaatg ggacgattcg atcgactgct
      721 gcatttggaa agccgatata tttgtcagta aatgatgttt ccggaagata tttatacact
      781 tctttttcta gaaatgctga accgccaatc atattttcac aggcaatgat atctaaagat
      841 gttgtgcttc cttccttttc tctttcttgg ataccttttg caatcaactc agcaataaaa
      901 ggtaagatat tcgggccaat ggcggtagta actaaatctg cttcagcgat ttcttggatt
      961 actttttctg gatttgtttg attgttgatt cctttgacat tttttacagt gattttttct
     1021 tttgattcat ctgctaattc gatttcgtat tcttttcttt catttaaagc atcaatgatc
     1081 gtgtgattca catctacgaa agtaatctca aatccattag ctgccaaaac ctctccaata
     1141 aatcctcgac cgatatttcc tgcaccaaaa tgtactgctc tcatttttct ctctccttaa
     1201 gcaattgcta gattttccgt tacttcttct ttgctgagcg cgtctgctag ctggacaaca
     1261 ttgtccatat cactgcaata aagtgcgatt tgctgtacaa gttgcaaatg ctcatctccg
     1321 actcctgcaa taccgaacaa aacagtcgca atcttttctt cttcttcggt accaaaatct
     1381 acaccatctg gtacttgaac gacacagatg ccggattttt tcacatattt ttttgcttca
     1441 tctgttccat gaggaatcgc gataaaattc cccatataaa cagataacat tttgtctcta
     1501 tctaccatgg catcgatata ttcctcatca acgccccctg cttctacaag catctgaccg
     1561 caatagcgga tcgcttcttc tttggtttta aaagatcgat tgagctcgat catgtcgaat
     1621 ggcaaacttt gcatgctgtt ttcctccctt aagctgtctg tttcaaatat tgtgcaatcg
     1681 attcgtattt ttgcggattg accatgctgt tgactgataa atgaatggat tgaggtgcag
     1741 ccatctgtac tttttccgct aaactttctt tggctacgat cagtgaatca ggttcatctt
     1801 ttaaatcttg gatattgact tttcttaacg ggatagttaa atggttcttg tctaatatat
     1861 ctttgagcac ggtcatcccc attgttgctg atccggaacg atcatcgtgg acaagtagaa
     1921 tctctttgat ttgtttgatg tttgctgaaa gaaactcttc tgctggctca ttctcaattt
     1981 cagaaactga tgttttctgc ttgctgctta attcttgaat gatttcatca tatcgaggtg
     2041 aattcaaaaa gttttctact gagacaaaac gcgcgctcgg tgctttttgt tttgctcttt
     2101 cttgtagttc gacctgagta acgatcaatg tattcgagtc atctgtcagt tggctgattg
     2161 cttgattggt caccgtttga ggcaagtctg cttcctgtac tttttttctc aagatcgaag
     2221 cgcccatcgc acttgatccc attcctgcat cacaagcaaa aatgattttt tgtacgccag
     2281 agaatgtagc tccttgagga ataccttggg cacctttgga agcttgctta gctgcctttg
     2341 tctcttgaac agatttttcg aagtcttcat ttgtatcttt cgcatccgct ttcaaaatta
     2401 ctgcagaaat accaaacgaa acagctgcgg caacaatcac gcccaaaata acaggaaggt
     2461 aagaattgaa cggtgtcatc gctagaatcg caatgatcga ccctggagaa gctggcgctc
     2521 ttagtccagc atccatcaat tggaaaatga aacttcctga cattcctccg cctataactg
     2581 ataagaataa taacggtttc atcataacgt aaggaaaata gatttcatgg atcccgccta
     2641 aaaagtgaat gatcatggct cctggtgctg aagattttgc tgctcctttg ccaaacaaag
     2701 taaaagcaag taatacgcca agtcctggac caggatttgc ttcaagcaag aatagaatag
     2761 attttccagc ctctgctact tgttcagttc ccaatggtgt caaaatccca tgattgattg
     2821 cattattcaa aaataagatt tttgctggtt caataaaaat acttgtcaat ggaatcaaat
     2881 gggcagataa aatgatctct acaccttttg ccattgcttg agtcaatgca tcgacaactg
     2941 gaccaatcgc agaaaaacct aataacgcta atccaaaacc gataagacct gctgaaaaat
     3001 tattgactag catttcaaaa ccggatttga tccgtttttg gaatagctga tcaaatttct
     3061 tgatggcata tccaccaaga ggtcccatga tcatcgcgcc tagcatcatt ggaatatctg
     3121 ttccgacaat cacacccatc gttgcaattg ccccaacaac tgatccgcgg tcaccagcaa
     3181 ttacttttcc gccagtataa ccgatcaata aaggaatcag ataagttagc attgggctta
     3241 ccatggtagc caaagcctca tttggtaaat aaccatctgg gataaataga gccgttaata
     3301 caccccaggc aatcagtgct ccaatatttg gcatgaccat gcttgataaa gtactgccta
     3361 atttttgtac cctcgctttt agcgaaatgt tgtttttggg aatcgtccgt tccattttgt
     3421 ctctccctct ctccttttga tactctaatt gtatccgctt actaaaaaga gaaatagtcg
     3481 tactttggca cgaaactttg tgccaaaggt ttgagtatgt cagcaaaata aaaagatttc
     3541 ggtatcagaa aacttcaatt ttaaataaaa ctgttatttt aattttttta taatatgcaa
     3601 acaataactt ttgttagcat ttaatttaga tgttttccat tctattatgt tattaaattt
     3661 tatattggag gcctatctat gacattactg atcaaaaaca tgaacatgaa acgtggtaaa
     3721 aaacgtgtga ttcgacaatt gaatctttta gtagaatcag gagaagtagc tgctttgatt
     3781 gccccgaacg gatttggcaa aacaactttg ctgaatggca ttgcccaact gctgccaaca
     3841 acttatgatc aattgtccct tcacaacatc tctcctactg cttatacaga ttatcgccgg
     3901 tctttctttt ttctagaatc aacgaatcag ttatccgact atctatcggc tgttgattat
     3961 ttgactttga tcaaatacta ttggcagagc aaagtagaat gccaaactgt tttccaacga
     4021 ttgaagatga ctgagtacct tactttgccg attggaaaaa tgtctctagg aatgaaacaa
     4081 catgtcttgc ttgctgctta cttgatcagt gattcatctg tcatgttatt cgatgaaccg
     4141 ttgaacggat tagacccagg aagtattgat atcttgaata gcctcatcag acaatggcag
     4201 aaagagggga agacgattct tctttcttcc cacgctatcg cgaatatcca atcgatttgt
     4261 tctaaagcat gttttctaaa aaatggagaa atcgtcacag aatcctcaaa tatggaagag
     4321 attttagcta tttatcgtaa attatatgag gtgtaactat gtctttactt acatttgaac
     4381 gcaatctctt actaaaaaac aaagtaaact ttttatttcc tctattgttg gtcgtgctct
     4441 ttgcattccc actttttttt gatcataaac ttgcttatac agaattcgat gaactgaatc
     4501 ataactatga agagatgcaa cgtttgattg aaacgttgaa agaagatgag aatgagaaag
     4561 aatttgtgga aagtttagag aaaagcaaca aactgatcga agcaatctta catgcaaaaa
     4621 acacagggaa tgtccagcaa atggtcgaag caacttatca ttatgaaaaa gatatactgg
     4681 atcgactgat ttctggtcaa agacaaggaa ttccaatcat tgaacaacaa aaacgtgtcg
     4741 agttgctccg ttatatgaaa gaacatcaga tccaaagata ttctattttt gacttgccag
     4801 cgcatctccc tttagcaaat tactacgaaa atattttttc aggaatgatt tctagttttt
     4861 tgattttatg tataaccgcc ttgtttcttt cttccatcat ctcttatgaa aaaagaaagc
     4921 aggtaatctc tctagtcaat ctattaccgg atagtatggt aaagaaacat agcatccgtt
     4981 ttaccattta ttatggtgca gctatgctaa gtctcgtgat gccatttttg atcgtctcta
     5041 tactagtaat aatcaaaaat ggtttgggag acttccgtta tcctgtagga acgatcattg
     5101 gtcaggagat acgaatcctt ccgatgtatg agtatctttt ccagagcttt ctgttcctgt
     5161 tgttgtgggt gctcttttta agtacaatca gttttcttct atctgcttta tttgaacatt
     5221 ctctagtcaa tcttttaggt acacttcttt gtcttttctt agcagagtat cgcttgtttt
     5281 cttcgattgg atggatagaa tcaatcagtc attacttgcc aacttcttat gttgattttc
     5341 aaaatgtgat cataggagga gatattttct cgccactagc ttcagaacag gttactttca
     5401 tgaatggtat cttgacactt ggtatatgga gtatcgtttt attattcata ggtatgggca
     5461 ctatctatat aaaaaagagc tattaaaaca caaaaataat aggggcggtg acaaaaacct
     5521 tgtcaccgtc ccctatttca aataaacggt tttctagaat aaatagtttt caaaagcttg
     5581 catccgcagt aataagaacc tcccaacaaa aatagctcct cttattttta tcttggttga
     5641 agttattact tgatgcaagg cagctttttt tactgtgaac attggttata tatgtccatg
     5701 ttcaaaaatc atttggactt cttcaatggt tttcgcatca ctgagcgcca ttacttggtc
     5761 gatgtctgaa cagaaaaagg ctaaatcttg gagcaccgct aactgatgtt ctttttttaa
     5821 agccactaca aataagagtg tcgcgatttt tggttcctct tttgttccaa agtccacacc
     5881 atcaggcact tggactaaat agattccttc ttctactact tcttcatctt tgtcagtccc
     5941 atgtggcaag gctacgaaat tcccgatata gacactcgct ttttcttgtc tttcgatcat
     6001 tgattggata tatgccggtt tgattccccc ttgttgggtc atccactggc cgatctcggt
     6061 caacgcttct ttccaatttt gataggattg atcgagcaat attttttctg ctggtagctg
     6121 tatcatcttt tcaccttcat ttctttattt cttcaatcag gattttcgct aacaactgat
     6181 aaacgatttc tttatttcct gagcggaaga tctcggtgtt caagtcattc atgatgattg
     6241 ctccactgat ctttcctaaa agaatcccgt ctttttcttg gattggcgaa ggcgccagca
     6301 taactaacat acgggacaaa tcgactggct gtctatccat cccttcaatg gtcaacggat
     6361 gtttcaaatt gaaaatacaa aagatcggtt tagaaatcaa tgagctggat gtatggaata
     6421 accccatatt tgtatgagga ataccgatag gtgcttgttc atatcgctta agcagccggc
     6481 gatgaaccac ctcttcatcc gttactagtc cttcaggaaa ataaggaaat aagctgcgaa
     6541 tcgtttcgga aatcgtctca ggattatcca gttcttggat aaaaaaacga ccgagtaatt
     6601 gattgatttg atccatgaaa tgcattgttt cctcataagt atcttctatc atccactcgt
     6661 tattgctgtt atacgtaatt ttttgccttt ttttcttctc aatatgcgaa aaagcttctt
     6721 ttagctgctt gacttcatct tctagcagta gcggggagac taaaatatat tttccaccgt
     6781 atcctggtaa aatagatgta gaaataacaa catcatattc ttcttgtaaa tcgatttttt
     6841 ttaaatcagc aatccgatag aaatgaattt gttggatgaa gggaaaatac cttttcaatc
     6901 gtagttccaa catgctagta gaagctaatc cacttggcga aaatcctgca atactgatat
     6961 tgctttcttt cggacttcgt tccaatgaat tcgcaaaatg aaggaccata taagcgatct
     7021 cttcttcgga taattttttt tcttgaaaaa tccgctctac tgctttacgg attgcattcg
     7081 caacttcctc gtattgaacc atgatttttt ctaaaatcgg attggtcagt gtctcttctt
     7141 gtaaaacggc tcttgaaaac acaccagata aatgagtcaa cagcattttg tacaaagttt
     7201 catcttcata aaaatggaca ttcgtctctt cacttacttc ttgaatcagt tgttttacta
     7261 aataagcaag ctgtgtatcg aacgtctctt caaagaaatt ctgatcaaat gagttcgtga
     7321 agtcatttaa taatcctgca aaaaagacga tctcatttac tggataaagt tgttttgtct
     7381 cagaagcgat ccgtgaaaac aatcgtttag ctgtctgtag taattctttg tttgccagac
     7441 tattatatgt ctcatttgtc agtaaatgat ttttagtcac acgctccatc gtcaatgtca
     7501 gcatcaaaac caaatgttca agctttctat cacttagatt ggtctttttt tggatcaatt
     7561 cttccccgat tactttgcgt gcaagagata gatgttgcgg atcaacgaat cgaaaaaaat
     7621 aattcagtga tgcatcgaaa gaaatactat gaaaaagttc atattcattg atttctaatt
     7681 ctaatacatt cgctgtcaga agccggcggt atttttccgg tccttcgatc tcgtatccct
     7741 ggttccgaat gatttccaaa ggcagttggc gaatactttc ttccaactgt ttgatatctg
     7801 cataaaaagt ggtattactg atgcgatatg attccatgaa atgagcaaga ctcaacggct
     7861 ctgtcgtcag taacaactcg gctaaaatcg cgtgttgtcg tttagcagtg gataagtcat
     7921 tacttttccc atctgtattt tcttttaatt gctctaatac ttcttctgat gcctcaatga
     7981 caaatgagcc tctagctgct tttttcaaag aagcatttac cgatttcaat gagacttcaa
     8041 gattatctaa ttcgcggtaa atagtccgtt tactgacctt caataaattc atcatttttt
     8101 ggatagatac aggtgacggg ctactgagaa attctgctaa taattgtttt tctctgggcg
     8161 ataaatacat cttatatttc tcccttcacg agtttttcgt attcattcgt agctacccaa
     8221 tgttcaacta gaactagccg agggatcttt tcttctagct gttcagttac agctagttct
     8281 ctttgtgcga tataaatcgt ttcttcttgg attttgattt tttctattga ttcttttgag
     8341 ataacaaact gttttttcgc tttttttgct aattgactta aaatcgtcat gcccatagtt
     8401 tgtgagccgc gtctgttgtt tttatacaaa aatacaatat tagcaggcga ctttttttgt
     8461 tctgtgatat ctgctatctc ttttttttct tcccgctttg gaaattggat cgcttctttt
     8521 attcgtgaca gcaagatatc tgtttccaaa aagtgatcca gtacatagat ttgactttga
     8581 ggtacttttt ctcttgcaag acccgctagt tcatgttgca cgataaccag ctgatttact
     8641 tggttattca attcatagat agagctgtat ccgacagaaa gatcgatttt ttctgctgct
     8701 aatttctttt tcagcagggc ggctcccata gcacttgatc ccatacctga gtcgcaagca
     8761 aaaatgacgg aatgaatgat agatggttcc aagatttctc ccctttcttt gaccgttttg
     8821 ttctcttttt ctttatttgg taatttcaat agtatcgctg cacaaatgaa ggtcacaaat
     8881 gtactgattg ctatccctat acttacccca gctagttgtg gataaggcgc attggcaaaa
     8941 atcacgagta gtgacccagg cgatacaggt gcagctaatc ccgtatctaa ccattgaaat
     9001 atcaacgtgc cgcttgcccc accagccatt acagctaaaa atagatacgg tcgcagcaag
     9061 acaaatggaa aatagatttc gtgaatcccg ccaatcgcat ggatcatcaa tgctccactc
     9121 gcatttactt tttcctgttt ttctgcaaaa caaaggatag aaagtaagat ccctaaacca
     9181 ggtccaggat ttgcctccat caaaaataag gaagagtacc cgactgtttg tgagaattcg
     9241 attcctaaag gtgtcaatac accatgattg attgcattat tgaaaaatag tacttttaga
     9301 ggttctaaga tcagactgac aagtggtaac agattcattg tgactagcca agacacaacg
     9361 gaggcgagca acaagtgaac actatttaaa actggttcaa taaaaacgat tccaattata
     9421 caaataacgc taccagtcag tcctactagt aggttgctga caagcatttc atatccagcc
     9481 ttgaatttca cgtggaatct agaaacaaat tgttcatata cgatgccggc aagcggcccg
     9541 ataatcatcg ctcctacgat ttgtggagca ttggaacgag cgatcaaccc taaagaagca
     9601 attgcaccgg cgactgcccc tttttgtccc cctaccgatt tcccgcctga ataactgatc
     9661 aagatgggta aaagcagctg gatcatccaa tggcatacct cttctaatgt ggtgtgcaaa
     9721 tcgccagaca caccttttgt tgctaatgac aagattcccc aagcgataaa cagacttaga
     9781 tttggcatga tcatttcatt taagtgatgt ccagcttttc tcagttgttt catagacacg
     9841 cgactgctgt tgtttgcttt ccatttgtat tccatctttt tcaccatact tctatttatt
     9901 gatggacgga ttttatgtag aaaaagcagc aaacgaaaaa ccgtctgctg ctttcagcta
     9961 tctgggaact atttgcctgg ttcagtcgca atgattgcta gttcaccttg gaccgcccac
    10021 tcagaaacgc catttggaat aatacagctt gttccttttt ctaatggata tgtttttccg
    10081 tcaacgacaa ggtctcctgc tccttcgatt accgtcatca atgtataagg ggcttgtttt
    10141 ttgaaagatg tgattccttt aatatcccac tcatatacat tgaaaaactc agtttctaaa
    10201 taagtgacga tcgatgaatt cccttttcgg acttctttga tttgtaattc tggtgttttc
    10261 gcagggacag ttgttacatc gatcgattgt tggatatgca gctctcttgt ttgtccttgc
    10321 gcatcttttc tgtcgtagtc atacacgcgg taagtcgtgt cagaactttg ctgtgtttct
    10381 aaaatcatga ttcctttacc aatagcatgg atcgttccgc ttggtacata aaagaaatca
    10441 ccttttttaa ctggtacttt cttcaaaagg tcatcccagc gcccatcttt gatcatctca
    10501 gctagttctt ctcttgtctt tgcatgatga ccatagatga tttctgcacc aggttcagca
    10561 tcgatgatat accagcattc agtttttcct aattcacctt catgtttcat cccataggca
    10621 tcatctggat gtacttgtac agatagatca tcttctgcgt ctaagatctt gatcaataat
    10681 ggaaacactg gttctgaagg atggccaaac aattccttgt gttctgacca tagttcatct
    10741 aatcttttcc ctttgaattc gccattttct actacactga caccatgagg atgcgcactg
    10801 atcgcccaat cttctccgat tttatcgtta gggatatcaa aaccaaagac tgaacgtaaa
    10861 cgacttcctc cccaaatttt ttcttgaaaa actggtttta aaaacatagg ttcctgcata
    10921 attcaaactc ctctcatttt tcatagctat tatagcatta tttcggtaat accgttttct
    10981 ctttcaaaaa gttttcaaca atttcatctc gacatatgag atactccgca cgatgatcga
    11041 caggatgtac gcgatttgac aaaaatatca acgcttcttg tgtttttata tcaatcaaga
    11101 aaaacgtacc tgtataacca gtatgaaaaa gaatcgcatg accgtcttct acatcctttt
    11161 ttaagtccca tcctaaagaa cgatagccat tttttttcgg tgtttgatct tgcagtaaag
    11221 aatcaatcgt ctcttcttct aaaaaacgac cattacggat ttttcccttg ttcaaataca
    11281 tttggacaaa agtaatgctg tctttcaaat caataaagag acccgcgtta cctgcatgtt
    11341 cagccaaaac tcttgctttc ggatcatgag tttgtccacg caaaacctca cccgaaggca
    11401 gttgttctgt gggaacgatt tttttggaag gaaaaggaag aaataagctg ttcatcattc
    11461 ctaatggttc caatacttgt tcttgaaaga tttccactgc agtttcttgg taaatttcct
    11521 ctagcatcaa cccaagaaga atcgtaccgg catctgtata ttttacttct tttcctagtt
    11581 gctcacctgg ttctagcttc aaataagcgg cgatcaattc ttcctgagat agcttatctc
    11641 tgtttggtat ccatgttttg atatcagaag tatgtgtcag taaatgacgc aaagtgattg
    11701 tctgattttc aaattcagga agatatttat gaagcggttg atcgagttct atctttccat
    11761 cttctaatag acgtaaaact aatgaagtgg ttgcaacgac tttcgtcagt gaggctgcat
    11821 caaacaacag atcttctgtc atcagctgct tttcaggaac gacttgggca aagccggctg
    11881 taaatgtttc tacttgattt tcttttatga aggcgactgc tacacctgga taaaatcctt
    11941 cttccatctt tttcaaaata agctgtttcg tgtgtggata catctttttc tcctgctttt
    12001 ctatcagatt cttacagtcc aagcttaaag cttaatctat gaggaatcaa ccttttttac
    12061 acaaaaaaac tgcgcgaaaa accgcgcagt ttcccccggt atactatttt ctctttctat
    12121 ctctagaata agcgatagta tgaccgttca taaaatgacg agacatgcga caatcgttaa
    12181 gaaagaaaaa gttaagcgtc gcatcgaatg tcagttttat tcttttcttt tggctcactg
    12241 acgaaccacc attcgattcc tatagcatca tagattgtga gtactgtgtg ctttttgtct
    12301 aagaagaaat caactgatag ttgctgcaag tgagcaatca gtccttccat gctttcagta
    12361 tcgatcatga atttcagaaa atcaagtcca atcacttcat ctgtaggcaa atcaattgcg
    12421 cctccgctag cttctgtcaa accaacatgg aaatctcctt cattcaatac atggatgccc
    12481 tcttcttcat tatgtacctt caaaccaagt acatgattaa gaaattggcg ttcttttgtt
    12541 aagttagtaa catttaaatg gattttttca aaataagaac ctgttgatag tttactgaat
    12601 ttttcttcag attttcttaa taatgcttct tgatccattg tttctggaat ttctctttgg
    12661 tcgttcttat tctcttcatg gtaaatttcc aattcattct cttctgggtc tacgagcaaa
    12721 atccccatac gtccatgatc ttctaaagct tctttgattg gataattcat tttacggata
    12781 cgacaaagga tatctgccat ttcttctgtg ctagggataa tcaatgaaaa acggttcagc
    12841 tttttgatca cccccatgtg atcattcgct ctaggacttt cttccagcag caacatttca
    12901 cttccagctt cttctgttcc taaaatggct aattcgtttt cttctctttt caaatcaaag
    12961 ccaacaacgt tcttgtaaaa atcaatcatt ttatctcggt ctttcacccg aatggctaca
    13021 ctttttaaat aggtggcagc ccccaattga aaatctttca tatttcttgc tcccctctac
    13081 gtcaagcttt attttaacga tggattcttc ttctttcaag caataacttt gattttgtta
    13141 aaaaaaatgt gagaaaattc gcattttgct tgttttttgt aaattaatga gtaaaaactc
    13201 ttataaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa
    13261 aatagctcct cctatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
    13321 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gatacaaaac
    13381 ggaaaaatag cttctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg
    13441 aacaccgttt attcggaaaa agggactgtg acacgacttt tgtcacagcc cctaaaaaaa
    13501 ataataaatt tgtactattc tatgaagctt cttcttcaga aaactgactg ttataaagat
    13561 ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt tcctgtttct acgatcgaac
    13621 catgattcat cacaataatg ttgtctgcat cgcgtattgt agacaagcgg tgagcaacaa
    13681 cgaaacttgt ccggttttcc aataggcggt tcatcgcttt ttgaatcagt atttctgttc
    13741 gtgtatctac actagatgtt gcttcatcaa ggatcaggac atctggattt gctaagaatg
    13801 cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat attgcttgca tcctcgttta
    13861 atacagtgtt atatccttct ggcaatttac gaacaaaatc gtctacgtga gcagctttag
    13921 cagcctcaat gatttgttcg tctgttgcat cgtcgttccc atatttgata ttgtcataga
    13981 tacttcctgt aaacagccaa gtatcttgca gtaccattga aaactgtgcg cgcaattcat
    14041 cacgtgaaag atcacgggta tctacaccat cgtaacgaat actacctcca gaaacatcgt
    14101 agaaccgttc aagaaggttg atcagtgtcg tcttccctgc accagtcgga cctacaatag
    14161 cgaccatctc accaggtttg acatccaagt taaaatcagt cattagcaag ttatctggtg
    14221 tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg tgtatctttt tcttctggaa
    14281 ggccagattt ttcattggac atctcttctt catccaacac ttcaaatacc cgttcggctg
    14341 aagcaactgt tgattggata gtgttcatca aacttgcaag ttgagtgatt ggttgagaga
    14401 actgtgttgt atattgcaag aacgcttgaa catcccctaa tgtcatgttc ccatttgcaa
    14461 cttttactcc gcctagtaca gcgacgaaca catagcctag atttttaacg aaattcatca
    14521 acggcatgat gattgccgaa ataaattgag ctttccaacc agcggcatac agattctgat
    14581 tttctttttc aaatacttcc tggtcttttt cagtatgatt gaatgtttta acaatggtat
    14641 gaccaccata tgtttcttct acttggttat tcaataaacc taaacttttt tgctgatctg
    14701 cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat gatcaagctt aaagggatca
    14761 tcaataacgc gactaacgtc aattgccagc tgattgaaag catcatcacg atgaccccaa
    14821 tcaatgtgac tgcactagtt acaaattgag tcaaattttg ctgcagcgtg cttgcgatgt
    14881 tatccatatc attgattgct cgagacatga tatccccatt tgtatgtgta tcataatagg
    14941 aaatcggtac acgattcatt ttggcttcca aatcatgacg taattcataa actgttcgtt
    15001 gggaaacacg tgtcataata aattgctgta aaaagttgaa tacagcagaa atcaaataca
    15061 tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa gtctatcgga aattttgtta
    15121 cttgttggcc ttgttgcatc tgttgagcgc cagccattac acctttgaat atttctgtgg
    15181 ttgctttccc caagaccttt ggtgtttgga tttggaagat cacggctgct attgctagta
    15241 caaatacagc gatgatcgca accatacgtt tagacatata gccaaacaat cggcgtaccg
    15301 ttccccagaa atttttaggc ttttcacctt ttacaccgag atttctgcca ggtccaccat
    15361 gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt tgttttactc atgctaagtc
    15421 ctcctctctt agttgtgaat gaacgatttc ttgataagtt tcgtttgttg ctaatagttc
    15481 ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc acaaggatca tatctgcatc
    15541 cattactgta cttactcgtt gagcaactaa aacggtgata ctttctttca tattcgtttt
    15601 caatgcttgg cgaagattgg catccgtttt gaaatccaac gctgagaagg aatcatcaaa
    15661 cacatagata tctgctgttt tgactaatgc ccgtgcaatt gccagacgct gtctttgtcc
    15721 accagagaag tttcctcctc cttgttccac atggctatct aagccatctt ccatctctga
    15781 aacaaaatct ttagcttgtg cgatttctaa tgctttccaa atcatttcat ctgttgcatc
    15841 cggcttacca tactgcatat tgtctcggat cgttccagag aacagaactg ctttttgagg
    15901 aacaaagccc attaattctc gcaaattata ttgagtcatg tcactaatgt ccgttccatt
    15961 tatttggatc gaaccagatt cgatgtcata taatcgaggg atcaaattga ctaaggtcgt
    16021 tttacctgaa ccagtccccc ctatgattgc gacgatctcg cccgacttgg cttggaaatc
    16081 aatatcttcc aatgcaagtt tttctgctcc atgatatcga taattgacat gattgaaggc
    16141 caatgttgcg ttttttccag caaaagaaac tgttttagga ttttcagggt catggatacc
    16201 gattttttca tccaacactt cattaatacg gtcagctgaa gcttgtgcac gaggcaccat
    16261 gataaaaata gcggaaagca tcataaagct gatcaagatc tgcatcgcat aagtcataaa
    16321 tgcaatcaga ttaccgactt ccaaagtcat atctgctatg tagtgtccac caaaccaagt
    16381 gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt ggcatcatca atgcgacgat
    16441 cgtattaact ttgattgccg tatttgtata atctttattc gctaaatcaa aacgattttc
    16501 ttcaaagcgt gtcttatcga atgctcgaat cactctgacc cctgtcaatc cttctcgaaa
    16561 taccaggttt aaacggtcag ttttcttttg catacttttg aataatggaa cagcaaagta
    16621 catgattccg ccgatcaaaa taatcattac tgggatgaca actaagaaga tttttgtcaa
    16681 ctgatgatct ttttgataag caagtatgct cgcgccaatc aacgtgattg gtgcattgat
    16741 catcatacgt aaaaacattt gtgtaaccat ttggatttga gtcacatcat tggtcgttct
    16801 tgtgatcaaa gaggccgttc catatttatc aaacgcatct tttgtcagac tctctgattt
    16861 tttatagaca tccgtacgca attgcttacc cagtttctgt gattcacgag tagcaaaaaa
    16921 tgtatttgcg atagcagcta agatactgat caaagaaaag ccgatcatca caaaacctgt
    16981 atgccaaata taatcgacat ctccttgcgc taccccttta tcaataatat tggaggtaag
    17041 tgtcggcaaa tataaatctg ccagtacttg gatcaccatg aaaactgcag ctgcaattgc
    17101 tgaagtcaag gagatccgtt ttacgatttt tagcatctag ttcccttctt tctatttata
    17161 tactccgttt ttcagccaag aaagtttcaa attgaattca ctgattgctt gctctactga
    17221 gtaatcttca gatgttgaaa ataagcgata cgaatgcgca attgttgtaa atacagtatt
    17281 catcactaat tggatcagta attccaattc atgatcattt tttactttca gcttcgtccg
    17341 atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt tctttatgtg gatcgtgagg
    17401 cgttttttgg cgactcgtat gaggagcgac ttttcttgag gcacggtaat ccatgttcat
    17461 aaacaagtta cgataaaaag aagcattctc gccttccaaa atctcataaa tcattttaga
    17521 aaaataggat tcgaatcctt tgaatagatc accatcggat tctttcatta gttgtaacat
    17581 atctttagag ctgtcacgtc tcattgtctc aaagtaataa aaataaagat cctctttatc
    17641 ttcaaaatac tgatagaaac tgcctctagg gatatccgct aatttgacga tattagcaat
    17701 cgaagcttct tttaagggta ctctagaaaa ctcgattctc gcagcctcga ttagtctttt
    17761 ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc atatggatcc tcctttcttt
    17821 caagatgaca cattgtcata tgacgctgtg tcattataac acctcctacc tgttttgcaa
    17881 gacatttgca tagttttttt cattatcatc cgatacatta aaaaagtgat aagaggaatc
    17941 cttccatttg aattaatgag agttgcccat taaaatagcc acatactaaa caaaggatga
    18001 gataaatggc ccaatcaatt agtgtccaag acttcaataa attgcctctc gatgctgaca
    18061 gcatcgtttt agatacaaga aataccttag attacacaaa aaaccactta agccactcgc
    18121 tttctatccc cgctgctatc ttgccaagac aattaaaaaa tctagatcca aagaaacttt
    18181 attatattct aagctataca ggcaggcgat cagaagcatt agccgatcaa cttactgcta
    18241 atggttttcg tgctgtttat atcattggtg ggatggaagc tcttcagtcg agtgttgctt
    18301 gaatattgct tgttagattc aaaagaggct gtgacaaaaa atcgtgtcac agcctctttt
    18361 ataccctgtc tttttttagt tatttattct tcaaccttat caatatgact tgatagcctt
    18421 ttctttttct catagttata acaatcatag caataaaagg agaaggtaag catatggcat
    18481 ctgttttagt agtcaaaggt catcccctaa ctgcagaaga atcgcgcaca gttaaggcat
    18541 tgacgtcttt tttaacaagt tataaagaaa atcatccaga tgatgaagtg actgttttgg
    18601 aactttatcg cgatgatatt ccggaaatcg atgaagaatt attatcaggt tgggaagcat
    18661 tacgtgctgg tgcagaattc acttccttat cagaaagtca acaacaaaaa attgctcgtt
    18721 tcaatgaatt gactgatcag ttccttgctg ctgataaaat cgttatcgca aacgccttat
    18781 ggaatctaaa tatcccaaca aaattaaaag cttggttcga taccgtcaat gtagctggaa
    18841 aaacgttccg ctatacagaa aacgggccag aaggattagt gacaggtaaa aaagcactgc
    18901 acatccaatc taatggcggt gtatacaacg gacaagactt tgcctctcaa tatgtaaaag
    18961 gtattttaaa ctttgtaggc atcgatcaag tagatcagct attcatcgaa ggaatcgatt
    19021 atgaccctga ccgtgcagat gagttgatgc aaaatgcatt agataaagct gctgctttag
    19081 gtaaatcatt ttaaaaattg agataaatgc atacaaaaga gacagaggaa aaaatcttca
    19141 aagcagcatt tgtaccttgg atattttccc atctgtctct ttttgttatt ctttctgctt
    19201 tttcaacgga ttttcttcat aaagagaaga gattgttgtg taccattcaa aaacatgagt
    19261 aatcactact ttagctgctg cataaacagg aatccccaag atcacaccga tgattccgaa
    19321 caatttccca gaagtcagca gaactaataa aatcgttact ggatggattg ctaactgatt
    19381 ccctagtact aatggagaga tcaagcgtcc ttctattgtc tgctctactg caaaaacgat
    19441 cagtactttg atgatcatga atggacctgc tacaatggct aaaaagatag ccggaatcat
    19501 cgctaaaaat gatcctaagt aagggatcag gttcaaaaaa cccgcaataa tccccaacgt
    19561 gacagcatac tctaaaccaa tgaccgcaaa accaatcata aacatgattg ccacagcaaa
    19621 agccacagtt agttgtcctc ttatataaga agaaacttga ctattcattt cactcagcac
    19681 attcaacgtc ggtttttgca tgcgtgtcgg taaaaatttg acaaaataag gtgctaattg
    19741 tttaccgtct ttcaatagat aaaataagat aaacggcatc gtcagcagag ccactaaaat
    19801 agtcgctacc gcgccgacaa aacttccaag actctgaact gtactcttag atatatcttg
    19861 gatcatatcg cctgccgaat tcatgaactt atcacctgct gtttccaact gttcgcgaaa
    19921 ttggttgaac agcgggtccc gcaaaatctc ggttaattta ttatcaatcg tatctacata
    19981 ttgagggaag ttactgatga agctcactgt ttgttcctga atcttaggga tgatcactac
    20041 gctcccccat gcgattagtg caacaaccaa aacaaacagc ccaatgatac tgtaaagcct
    20101 tggtactttt cgcttttcaa agtaatcaac taccggattc atcaagtaat acaagattcc
    20161 tgctaaaatg ataggcagac ctacgattgc taaaaattgc cagattggtg caaacaaata
    20221 tgaaactttt gtaaataaaa agacgatcag caatattaac aatacaacaa gtaaggctgt
    20281 gactacttga ttatttaaaa accagcgcca aaaccacgat accggtctgt tcttcttatt
    20341 ttttccttcc ataattcctc ctgttagtta taaaaaattt ggcttcccaa accaatagca
    20401 ggcagtctat gcggatgaag atagattcca tttgcaattt gtccttcttc tggacaagca
    20461 tcaaaaatca atgatacgtg agctactgtc tctaaattcc cattggccat cggaccgaca
    20521 taatcgatcg tatatacctg atcatcaaaa gcaattgttc cgcctgcttt caaagaaaaa
    20581 ggttcgtctt ttgatacttc ttgaatcacc gaatattttc gtaaggcagc tgttgctgtt
    20641 ttatcgaaca aaatcagcat gggttcttcc ttgtttattg cttctgtccc aatttctgta
    20701 atctttgcct gaatcattgc actctctcct ttaattgctt tcaaaaacag tataacacat
    20761 tctcattctt tagccgtgtg gaataattat gtgatagaat aaaaacgaaa actatttcat
    20821 tacaattagg aggaatcagc tcatatgatc gaaaagataa ttttaggaac ctatacgcgt
    20881 cgcgaaagta aaggaatcta tacgatcgac cttgatacag aaaaagaaga attagccaac
    20941 ctttcattag ctgttgaaga aaatagccct acctatgtag caaaaagtaa agctggcaat
    21001 ctttacacag ttactagtgt ggatggatta ggtggtgccg gtgcgtacga taaggatttt
    21061 cattttttga atgcggtaac agagtctggc gctcctttgt gttatgtagc ggttgatgaa
    21121 gcacgtcaat tactatacgg ggcgaactat cataaaggtg aagtaaatgt ctaccatata
    21181 ttaggagatg gcgaactgaa agcagcagat tcaatattcc atcaagaagc gacaggtcct
    21241 cataaaaacc aagatcatgc gcatgtccac tatacggatc tgactcctga ccaacgatta
    21301 gtcgtatgtg atcttggtac agatcgtgtg tatacttatg atgtgtccga aaacggcaaa
    21361 ttgaatcttg taactacctt cactgcagag ccaggaacag gccctcgcca tcttgtcttt
    21421 catccaaatg gacaattcgc ttatttattt ggtgaattag acagcacagt ccgcgtgctt
    21481 tcttataata aagaaacagg tgcttttgaa gaattacaga aaatatcgac cttgccagaa
    21541 gaattcgacg gtgaaaatgg aggggcagct atccgcattt caaatgatgg caagttcctt
    21601 tatgcttcta accgaggaca taactcgatt gctgtattcg cagtttttga agatggttcc
    21661 cacattcaaa acaagcaaat catctcaaca gaaggggatt ttccccgcga tttcgcacta
    21721 gatccaagca ataattttgt cgtgtgtgcc aatcagaaca ccgataatct gacattatat
    21781 cgccgtcatc ctgaaacagg attactggaa ttactgaaaa aagatgtcta tgtaccagaa
    21841 tgtgtctgtg tttattttga tcgttagtaa gaaaaggaga atgaaatgaa tcagcaagac
    21901 gtattagaca gactacggga agaactaaat attcctttct ttgatgggaa aatcgaagat
    21961 aaagagtata gcgaagagga atatcagaaa ctcaaagccg atttacagaa ctactttgaa
    22021 caatatgtac agaatgtaga aaactaatca gctttccata ctatctgaaa agaacatcag
    22081 gacaagactt ttccgtctat cctgatgttc ttttctacaa ataaaaaacc gccgagatgg
    22141 ttagactcga cggaaaagga gtatttactc gattttgagt aagtacaagg ttgttgttgg
    22201 ttataggtat gtcattatca taaaggattt tgatcactct atcataaagg taatattaca
    22261 tttatattag ataactctag tttttattgt tatcaaagat ttcttttatt ttttcaacaa
    22321 caaattagtc attttttgga tggcagctgt caattggtct gtcgcttggt cttgttttgc
    22381 ttcagatgta ttgaatagat cttcatctac ccctgattca agatcgatac tttggatcat
    22441 ttcttcacag cctgctacat atgaaagata agctttttca aaatttttat gaatgcccat
    22501 tgcttttgct ggtggacgca aagtactgac tttcttaagc attgcccgat atttttttgt
    22561 tccttcttca aataacagga tgatctcttc aagagtcgat ttgtccagtt cacttgtttt
    22621 tccttcatca attgcgtttc tgatcacttc atatttcgga tgcattcctt ctccgattgc
    22681 ttctgtttcc ttgacgattt gatcgatgga ctgcacataa tagccaatat tcggtaacat
    22741 gttcgttttc ctactttctc cattgttgtt attatttatt ctacttgttt ttatcctcaa
    22801 atgacaagta cttgaaaaaa tttaatggtt tctatacagc tcgccattct tattcgtgct
    22861 caaagaaacc atctgctaaa ctatattgtt tccctgcgac aatgtcatat tggatcaatt
    22921 gatattcgtc gtaaatctct tgttgcgagc gattgaattg cgcttcttta taccactgtc
    22981 cgttttgata gtacagttct cgttcaaacg ccggaagttc ctgttctaat gataaaagca
    23041 gctgataaaa gccatttgtt gtctgattcg tctgttccat tagattcgct gcaaaataaa
    23101 acggactggt gattgcatcg tgagtcgttt gcttttctaa ttctccttta ctgtcgaaca
    23161 taagaaattg tgtttcatgc aatgtttgtt tgtcattttt tgcttggatc gtatctgaat
    23221 agatccctgg aagatgatcc ccccaaaaaa caactaaagt ccttctagaa agattcttca
    23281 attcttccgt gaactgcttc agtgccgtgc tgctatagga aatatcttgc agataattct
    23341 ctaagtcaag tgtcccactt ccatctgaag tttttgcagg atagtctaga ttatcatact
    23401 ttccatcgta gggcatatgt gtctgcatcg tgactaaatg aatgaactga ggtgtttttt
    23461 cttccttgag aagtgtcatt acttctttgt aagctgactc atcagaaata tatggattat
    23521 tctctatcgt atccgtatag gtcatcgtac gctcgctgat gaactggtca aatcctaatg
    23581 tctgataaac atcctctctt ttatacatcg aagtattgta tggatggatc gctgtcgtct
    23641 ggtatgattg tgcgtttaac gctgaaacga tagaaggaag ctgatccatt tttggtacaa
    23701 gcatcgtgta aggggtggtg agttgcgcat taaataacgc catagaaaaa ccagtcaatg
    23761 cttcaaattc gatatttgct gttccaccgc catagttttg agaaagcata ttgccactat
    23821 atgtttgatc agctacttca taataatctg ccaatggctc accagatact tctacgccgt
    23881 ttaatcgact aggatctgag aaactttcac tcatcacgaa cacgatgttc ggcgactctt
    23941 cctctacagt tttatttttt tcatcggcta gcttctggta tttttcagtg atttctttga
    24001 tcttttcttt cgaatatcct tcaggttcat ccattggttc taccttcaga ttgtacaaaa
    24061 acccaccgat aaaaccagtg ttatagtagt tcattttctg gctgtaaggt atccatagtg
    24121 ccgttttatt atatgctttt cgcaacaaat tatcgggatt gttgaaatta ctgatatacc
    24181 ctagcagtcc aattgccaca agcagtgtcg ttaatcggat caattggaat cgccttcctt
    24241 ttttcaggct tcgaaacatg taccagcaaa acaatcctat cacggttcct gcggcagcta
    24301 agatcactgt aaacagcata gtgccggtca tatctttcag caatccgatt tcagtgatca
    24361 tcttcaaatc gtctggataa atcggttctt gccgataact cattttcata taatttgcaa
    24421 aacctaatac accgatggct acgacatacg taagggaacc taaagggaca gatcctgcta
    24481 gtgcaatcag aaaaataaga ataatcagca agacgagaca ggctaagaag aatttttccg
    24541 tatgccaaga aaaagcaaat ttcaacgcaa ggtctacgga tagatcattt tgacaccact
    24601 gcaaaaataa attactaaaa acaacaagaa aaaagatact gatgattgct gctgttcctt
    24661 ttaaaatctt tttctgtttg gtcgaaagtc tgcttatata tcttttcaaa tgcttgctcc
    24721 ttcatttcta tcaataaaag aatgttgctt ctcttatttt agttgtacga aattcgaaag
    24781 taaagaacgg aaagaagaat taatgaaatc caaatattct ctttctttat ggcctttctg
    24841 gtttctttaa taagcggacc tttcgtactc cagaaatttt ttctagctgt tgtttcactt
    24901 gattgagctg ttcaaaatca gtactgtcta tgtcaatcaa tatgtagaca tgctcattct
    24961 tatggttacg cgatatatct gcgatcgcta gctcgttctc attcagcaga gaaaatatct
    25021 ctgctaatat attggttttc ttttgataaa atatagtgaa tcgaaacggc gcttggaaaa
    25081 acattcgtgc ggatggataa ttgaccgctt ctctaaccgt accgaacaaa agataatctt
    25141 ttaaggcttc aactgccaaa cgtccgctgt ctgccaaagc ttcctgagtc gatcccccta
    25201 gatgaggcaa cataagtata cggtcatttt ctaagaactc ttcttctgga aaatcagtca
    25261 agtatttagc cagatgttgt ttttccaatg cttgcataac agcatattta tcaacaattt
    25321 cacttcttcc tacattgata agtacagcat cttttttcat tttttctata ttcgcctgat
    25381 cgatcaatcc ttttgtatct tctgtcaacg gcagcatgac tacgatgtaa tccgaggtag
    25441 aaagaactgt ttctaaatct gcttgccgaa cataatccaa ctgggcatcg cggatagaat
    25501 aacccaagac atccatccct aagctatagc aacttagcgc tacttttgtg cctatcgctc
    25561 caagaccaag aagaccgatg gttttgcctt gcaattctcg tcccacataa gcacttcttt
    25621 tgttttctgc ctgttctaaa atattaggac ctgttagtgt ttgtaccata cgacttgctt
    25681 cgattatcgg acgggaagat aagagcaaac agcacaaaac aagctctttt actgcattag
    25741 catttacacc tggtgtattc atgacgatcg tcccgttttc agaagctttt tcaacgttga
    25801 tcgtgttcac cccaacacca gctcttgaaa ttgctagcaa atctggtgta agccattcat
    25861 ctttcacttt cgtactacga ataactattc cttcggctgg caaatcatct attattttga
    25921 aatattcccc ttgtaacttt tctatctctt gttctggaat agcatcaatc aaatgaacgt
    25981 gatacataaa accccctcct tactaacaag tatctcgaaa tttctgcaat tagacaactg
    26041 tttaccattt gacttcctag aagcaaaaag agcagttaga gcagagtagg ctgagtgata
    26101 aaaataaggc tatgacaagt tactgccata agccttattt tcgagagcaa aatattgagg
    26161 gtgagatatc ttgataaatg gtgttccgtt agctgacatt gataataaga ctaaatttca
    26221 aaaaagttga aaagcaaatt tcgtcaatct cttcttattt cttaaaacta agtggctttt
    26281 tcacaacctc tttttatcta ataaccactg tttccaactc aactaatttt gcccacgttt
    26341 caatttgatc tgttgttaga tttagcgaga caacggtatg atgaccgcca cctgcctgaa
    26401 tccatgaatg gacaccttca tggaaattcg gtttagtttt ccataacaca cgtgcaacag
    26461 gtaaattagg tgcttcagtc gttggttcaa aggcttctac ttcattgatc agcagtttat
    26521 aatgcgtacc gaaatctgcc atcgatacga ctaccccttc tcctgctttc ccatcaaata
    26581 caagacgcgc cggatcttct ctatctccca ttgataaagg agaaacaacg attcttggtt
    26641 tcgtcgcagc aagcgtcgga tctacttcca tcatatggga ttccaagatt gcttcttggc
    26701 cactagctag ctcgtaagta taatcttcca tgaatcctgt atctttccct cgagccatga
    26761 ttttcagcaa ccgatcaatc gctgcagttt tccagtcgcc ttccccagca aaaccgtatc
    26821 cttctgccat caaacgttgt acagctagtc caggtagttg tttcattcca tacaggtctt
    26881 caaaattagt cgtaaaagcg ttatagcctc cttcttctaa gaaacggcga ataccgattt
    26941 cctgctgagc ttgtactttt acatgttcct cccatgtctt ttcttcataa tcaccatagt
    27001 cgaaggtata caattccttg tactcttcaa ataattgatc gacttcttcg tctgatacgc
    27061 gatcgattac ttgtacaaga tctccgatac caaaataatc aacagtccaa ccaaattgaa
    27121 tctgtgcttc cactttgtct ccttctgtga cacccacgtt tctcatgttg tcaccaaaac
    27181 gagccacttt tattccaaaa ctttcattat aggctactgc aacatccatc caatcagcaa
    27241 tctctttttg gatctcaggt cgtttccagt agccaacgac gattttattt tgtttattta
    27301 atcgagcatt gatgaaccca tattcgcggt caccatgagc ggattgattt aaattcatga
    27361 aatccatatc gatcgttttc caaggaatgc tttcgttata ttgcgtagct aaatgaagca
    27421 atggtttttg tagaagcttt gtcccgcgga tccacatttt tgctggtgag aaagtatgca
    27481 tccaagtgat cacacctgct acttcttctc gataattgac ctctttcatg atttttgtga
    27541 tcgtatcagc agtaactgct aattcttgaa ggcggattgg ataaggcaat tggccatttt
    27601 cattcagttc atcgaccatt tcttgtgcat gtttctttac ttctcgcagt gcttcttccc
    27661 catataaatg ctgtgatcct acaacaaacc aaaattcttt ttctccaata ttcaacataa
    27721 ttagccactc cttttatttg ttttgtccgt aatacgcatt ttttccgtgt tttcgtaaat
    27781 aatgtttatc taaaatccgt tgaggcagct cttctgaaaa tgaattgagc tgtctggtaa
    27841 ataaattcat cttgcatact tcatctagta cgactgcgtt cattaccgca ctttgcgcat
    27901 ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac ccctggaatt gctaagggat
    27961 caattttgcg ttctttgaat gtttctatga taacttttcc tgtttcatat tcgtacccgc
    28021 tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac cgatccataa aaagtatctg
    28081 catgcgtcgt ccccatcgca ggaacatcca atcctgcttg tgcccagatc gtcgcccatg
    28141 tcgagtgagt atggacgatt cctccgattt gaggaaatga acgatataaa acagcatgag
    28201 taggcgtatc cgaagaggga ttgagtttac cttcaatgat atgattgtcc aagtccacaa
    28261 cgaccatgtc gtctgctgtc atctcttcat aactgactcc gctaggtttg ataacgaaat
    28321 atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt atatttgacg agtccgtgtt
    28381 taggcaagtc aagatttgct tggtatactt cttctttcag ttgttccagc attttctatc
    28441 actccttttt cttgcaattg atgaatcgct gttttttcaa tgtcgatccc ctcacggtaa
    28501 cgtctcataa atatctgata gcctgctatt tcttctgctg aaggtgtgat ggtgattcct
    28561 tggtcttgtc caaacacgtc ttctgccaag aagttttcta aagacttccc atgtcgatcc
    28621 atcatatagg ctgctaataa cgcgattccc cacgctccac cttctccggc tgtgtccatc
    28681 actgtaactg gtgcctccat agcagatgct agaatccgct gtccaacttc tggtgtttta
    28741 aaaatccccc catgccccac taaacgatca atttggacat tctcagattt caatatatcc
    28801 attccgattc tcattgcacc aaaagcactg gctaaatgga tccgcatgaa attcgctaaa
    28861 tcaaaacggc tacctggtgt acgtacaaat aatggccttc cttcatttac tcctgtaatg
    28921 ttttcaccgg aaaaataacc ataagaaagt aatcccccgc aatccgtatc gccttctagc
    28981 gctttgttga ataataccgt gaataattta tctctgctta tctccatacc caaagaattg
    29041 gtaaattctt caaaaatact gacccaagca ttgatatccg aagagcagtt attcgtatga
    29101 accatgccta ctagttctcc tgaaggtgta gtgaccatgt cgatttccgg atggacattt
    29161 tctagttctt tttctaatac gatcatagcg aaggcggaag taccagccga aatgttccct
    29221 gtccgtttgg ctacactgtt cgtagcgacc ataccggttc ctgcatcccc ttctggcgga
    29281 caaaccggga ttcctggtct caaatttccc gtagggtcga tcagataagc ccctgattcg
    29341 cttaacttgc cagcttcttc tcctgctact agaactttag gcaaaatgtg atccaagtca
    29401 atcgctacgt gcgctttttc gattaactga ccaaaaatat ttttcatttc ttgatggtag
    29461 gttttcgttg aaggatcgat aggaaacatg cctgatgcat ctcctacgcc tagtactttc
    29521 tcaccagtaa gctgccaatg gacataacct gcaagcgttg tcaaaaaatt aatttctttc
    29581 acatgttctt ctttattcaa aatagattga tacaaatgag cgatactcca tctctgcgga
    29641 atattgtatt gaaatttttc cgttaatatt cgttctgctt cttcagtgat tgtatttcgc
    29701 caagtacgaa atggtactag taactgttcc ttgctatcaa atgctagata accgtgcatc
    29761 attgcactga acccaatcga cccgattgtc gtaagattct cgccatagat ttcgaatact
    29821 tctgctgcca tttttcggta actgacgcgc agtcctttcc aaatatcttc taatgaatag
    29881 gtccagatac cattttctag tttgttttcc cactcataac taccagcggc aataggagta
    29941 aaagatgaat caatcaatac agctttgatc ctagttgatc caaactcaat ccctaaagat
    30001 gtacgaccat ctatgacatc taacatactt tgcgcattta cttccttcat aatgatttaa
    30061 ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta gtagcgctta caatgattat
    30121 tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa gaatacaata aaaaacactt
    30181 gttttgtacg tacataagtt ataaaatgaa attgcttaca aaatctgatc aaccgccttc
    30241 tcgaaattct agaaggagag aatacttatg aaaaaagaaa aaaaagtttc ctcatcgttt
    30301 atttattttt ttggcgcatt tggcggcatt ctatttggat atgatattgg tgttatgacc
    30361 ggcgccttac cttttttaca gcatgattgg ggtttggcag gtaaagcttc tttgatagga
    30421 tggatcacgt cttcggtcat gttaggtgcg atccttggcg gctctttatc tggtcttctt
    30481 tctgataagc ttggacgacg aaaaatgatt cttctttctg cacttatttt tatggctggt
    30541 tctgtccttt ctgcatctgc ccctcacaat ggttcttatt ttctgattgc agcaaggatt
    30601 cttctaggtt tagctgttgg cgctgcatct gccctagttc ctgcttatat gtctgaaatg
    30661 gctcctgcta gactaagggg aagattgtca gggatcaatc aagtgatgat tgcttctggc
    30721 atgctccttt cctatgttgc agattatttg ctaaaagtgt tgcctgaaac gatggcttgg
    30781 cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt tatttttcgg tgttttagct
    30841 ctaccagagt caccgcgttt tcttatgcaa tctggccgct tggaagaggc aaaaagagta
    30901 ttgaactata ttcggacacc aaaagaagct gaacaggaat tcgaacaaat ccagctgaat
    30961 gtcaaacaag aaaagacaac cggaacttct tggcatactt tgtttttaga aaaatatcgt
    31021 tccttagtca ttgccggtat tggtgtagca gtattccagc aatttcaagg agctaatgct
    31081 attttttact atattccgct gatcgtagaa aaagcgactg ggcatgcagc gagcgatgca
    31141 ttgatgtggc cgatcatcca aggaatcatt ttagttgctg gttctttgct ctttttggtt
    31201 attgcagaca agttcaatcg gcgaacttta ttgaaaatag gcggtagcgt gatgggactt
    31261 tcattcatcc taccagctgt tttaggaact gtactggatg ctcatacaaa ttcgttgttg
    31321 attctcttgt ttttatgtat ttatgtcgca ttttattctt gcacatgggc tccactgaca
    31381 tgggtaatca taggcgaaat attcccacta gctgtccgcg ggcgagcatc tggcctagcc
    31441 tcttctttta attggattgg ttctttccta gttggcttgc tttttcctgt gatgactgct
    31501 tctatgtctc aagaaatcgt tttcggcatt tttgggatca tctgcttttt aggggtttta
    31561 tttattcaag agatcgtacc agaaactcgt gggaaatctt tagaggaaat cgaacaaagt
    31621 gcatctaaaa aaacttatcc taaacgtatt tctatccatc attcttaaag aggttgtgac
    31681 agaagtggtc agcttcaaga aataagaagg aaccgcttag tttccagtca caaggaacaa
    31741 tttcaaaaaa cagcttctca tgtttgatga atattgtgac ttgtgaccag ttggtaggag
    31801 aacaccgttt agtggagagg tggtgaaaga aacgttcagc cttgagcgat gacaaaagct
    31861 ctcacaaaca ttgcgacgag taaccgtagg agcaataaga ggcggtaaaa acccgtctct
    31921 tatttttcgt tttctttcac tgtttcccgt tcaactagta caggttcata gagaatattt
    31981 ggctgtgcct gtcctttttc aatcgttgtg acgatccacc gtgcggcatc ttgtcccaac
    32041 ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc ctaaatggct taaagaagaa
    32101 tcgtctgttc ctacgatcga taggtcatct ggaatatgat agccattttc tgtcaactta
    32161 tctagcatta aactagctat ttgatcgtta tagcaaatga tgccatctaa agcttgttct
    32221 tctttcaacc gttgtactgt tttttcagcg attgtttctc gtgtttccgt ggtgaaagta
    32281 atgatatctt ccgtcaaaaa acgcacatta tactgttcac acgcgctgat aaaacctttc
    32341 atcctgtatt ttccttgaag atcgtcgatt tttgtgatga acaacagacg ctgatgtcct
    32401 ttttttatca aatattctgc tgccataaaa ccgactttca catcatctaa acaaatataa
    32461 gaggcattta actcttcata agcagcattg atcatcacca tcggaatgcc ttgttctctc
    32521 aaagcaacat aaagtgctaa gtttgggtta tactgattac ttttcgtagg ctcaacgatc
    32581 aaaccatcta ccccataagc aatcattttc ttcaaacact ctttctccaa ttcgtgatcg
    32641 ttattcgtac tggctaaaag caatgaatac ccagattctc gtaattcatt ttcgattcct
    32701 cggataatcg acggaaaaat ataatcagag agatatgtgg tgatgacacc gatcgttttt
    32761 gttctgttca atggtgtttt ttctttctta tagctgtcat ctacatatgt gcccgaccct
    32821 ttttctttac gcagatagcc ctcatttacc aacacagcga ttgcttgccg aatcgtatgt
    32881 ctgctgacgt catatatttc ttgcagcttg aattctgagg gaatggttgt atttacttcg
    32941 tattcctcac gaaggatctt ttgttttatc tcttctgcaa tatcttgata ttttgccatt
    33001 tgccttgccc ctttttattt gtacgtacat atatcataac gagtctgcaa ggtttattca
    33061 agcaaaaatc aaggtttctt gtgctcataa ggcatacgga acaggttcac caaattctgg
    33121 aaatccttgc tcattccacg taaagcattg cgcgttcgcg tgccgattag gattgtctaa
    33181 cggatcccct ttttggttct tctctggtcg agcatgatag atcaaaatat caaagcaccc
    33241 gtcttctgaa accgtaaacg agttgtgacc aggaccaaat tgcttatttt tttcagaaga
    33301 aacgaatact ggctccgata atttatgcca tgattctttt aataaaagat tcttgtgctc
    33361 atcagcccac agcaggccca tggcatagtt ttcatctgtt gcactaccag aatacgtaat
    33421 gaatattttc ccattgcgag taaggactgc tggaccttca tttactgaaa aaccccgttt
    33481 ttcccattca tactcaggta tagaaaggag gatctgcttc ccctttaacg tccaaggatt
    33541 atccatttcg gatagataaa gattagaatt cccagtaatt tcaggatctt gttgggccca
    33601 cacataatat agctttcctt gagaagagaa agttgttccg tctaaactga aagtttccct
    33661 tttcgttttt atttgtccct tttctatcca atctgtattc attggatcta tatgctgatt
    33721 ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt atagttttat gattgcttgc
    33781 agcaaagtaa atataccatt ttccattgat ataatggact tcaggtgccc agatcaattc
    33841 actcatcggt ccttcttcat gagcctgcca tacaatcagg cttgcagcat gttctaattc
    33901 attcagtgac tttgcccttc ttaattcaat cacttggtag cctggaaccg atcctgtaaa
    33961 ataataataa ccatctgtat gtttatatac ccaaggatct gcccgttgcc gaacgatagg
    34021 attattataa agtgtcttca tcataacaaa tttcctcaca ttttctttat tctaattaca
    34081 ttccaagagt atttttctat ccctacattt aattcgtcat gtttttctat tgaaaaatag
    34141 tttatttcag gttttatttt ttctggttct tcacgtgtat ttttgtccaa cagatcgcta
    34201 ctggttaatg tgtagtgctc tccttgatct agcaaattga aatcatgttc aatcgttatc
    34261 ttttgttgtt catttgtgcg attgatcaag aaaatagtca gctgattgcc gttatctacg
    34321 atcacactgt ccacataagg aacatttttt atcttttcac aatcataaac agggcaaatg
    34381 atttgttgtt tcaatacttg acctttagca tatttcgata agtgttgcat cacataataa
    34441 gttggattcc tccatgcttg tcctttagca tctgtcagta ttaaaggtat tgtgtttact
    34501 aacaatgatt gacaactaat ctttacgcga tctgcgtgtc gtaaaatagc cagccctaat
    34561 gaaccaaata ataatgtatc ttccatagta aagtaacacc aatcgatggg actaccgact
    34621 tcaaaatccc tatgtctttt ttccggttgg gcatgtacat tccattcatc aaacgctaga
    34681 tacattgttt tctcacttct caaatatcct tttacataat cacaagtagc aacgatttct
    34741 tcaatttgac gatcgaatcg ataagctttt gcaaggtatg tcgctgtatc atcacgctca
    34801 tttttaggag gtttagtcaa gtcttcttca tcatacttat caatatagtt atgcaaagct
    34861 aaataatcta cattctcata agcttcttct aatgcgatac gatcccattc agggtacgat
    34921 ggtaaacgtg gcgtagagct tccaactaag attgtttcaa ttgaatcatc cactaacttc
    34981 atgacttttg aggcttcatt tgctaaccta ccatattcat aagcactctt agcccccact
    35041 tgccatggac cgtctagctc attccctaaa caccataatt taacgccaaa aggctttttt
    35101 tgcccatttt ttattcttaa atcactccag aaggttcctt ttggaaaatt acaatattct
    35161 actaaatttt ttgcttcatc aattcctttt gttcctaaat tcaccgtgaa tatcggtttc
    35221 gcttgtacat gactgatcca tttaaaaaat tcgtggagtc caaaagaatt tggttctata
    35281 cctcgccatg ctaaatcaat tttttttggt cgttgttctt tcctacctac agtatccacc
    35341 caatcataac cagatgtaaa gtttcctcca ggatagcgaa tgacacctaa ttctaattcc
    35401 tttacataag agaggacatc ttgtctaaac ccgtcaccat cagctgtttc atggcccggt
    35461 tcataaatcc ctgtatagac gacacttccc atatgctcaa caaatgagcc aaacagccga
    35521 gaactgatct ctcctaccgt aaaatcctct ctgattttta ctttcacttc ttcgtccaac
    35581 agctttccct acctttcatt tcctatagtc aagataatag atacaagttt ccatacgcca
    35641 ccagcaaaga taaaaataaa aggagggaaa agaaatatga gcagtacaaa tcctaataac
    35701 aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata ctttccatgc actttgttca
    35761 aaaatttgta aaaatgtgat atctttgttt tcagaaatca catatatcca ggataaaaga
    35821 aacatggaat aaataagtaa aactacatat ccactaattg aaagtacgat tgtctgttct
    35881 tctaataatt gattaacaag aaaataaata atgatcgtta acagactaag gaacaagttc
    35941 ttaccaaaat gttgctttat tccttgaaag aaaaaataaa tgatatttcc tgatccctca
    36001 cctttccatt gcgccattac ataaaaaaca gcacatgtag caggcacgat agtaataaca
    36061 ggcaaacaca cgattaccca gagaatacct aataagatgt aatcagaaac cacaatcaac
    36121 tgtccataaa atttagtctc aactattttc atcttcctca ctctttcatt ccagacatag
    36181 ctgttccttg aacaaaatat ttctgtgcaa aaatataaac cactagtaat ggcagcattg
    36241 aaataaccgt tccagccatt acaggtccat aaaaagaaat gttctgccct gtaaataaag
    36301 ctaaccctgc tggtaaagtt ctcatttttg aactacttgt caatatcaat ggataaagca
    36361 agtcattcca actattcatc agcgtgaaaa tccctaatga taaaagagta ggtttgctca
    36421 atggcaacat gattttcaaa aaaatattaa actcacttaa tccatcaatg cgtgctgcct
    36481 cttccaaaga atctggtaaa gtaataaaaa aggaacgcat catgaagata ccaaatgcag
    36541 ttgtcatacg aggaatgatc aaacctgcat aagtatcaag catgccaagc aaattgactt
    36601 caataaatag aggaatcatg aagacttgga atgggatcat cattgtcagc aaaacgaaat
    36661 agaacaaaac tgattttcct ttgaaacgca tacgagcaaa tgcatacccc gcaagagaat
    36721 caaagaatac agaagtgatc actgtccctc cagcaaaaat cactgtattt ttgatgtaat
    36781 ctaataaagg gatcgttgtc caaaccttaa tatagttatc tagggtatag ctatctgcaa
    36841 atattctagg cggaaatgag ataatgtctt tttcgtgttt aaaagaagaa ataatcagcc
    36901 acaaaaaagg aaacgcaata accaacaata taactagcat actaatcgtt cgaatgattt
    36961 ttttaccact aactgctttt cgactattca cagtaactgt aatcgagctt tctggtgcca
    37021 tacaggtttc cctcctattt catttgtttt tctttccgtg acatgtacac attagtggag
    37081 atagctatag ttgcaacaat gataaacaaa accgaagaca gtgctgatgc atagcccaat
    37141 tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa cagctgtttc tgtcttaaat
    37201 tgtgggccac cagcggttgc aacatatact tggtcaaaca cttgcatcga accaatcaga
    37261 ttcgttaaaa tacaaaaacc taaggaggga acgatctgag gtagcgtaat gttaaaaaac
    37321 tgctgtcgat ttgttgcacc atccatttga gcagattcat acaaagaggg agagattccc
    37381 tgaatagcag tcaagagaat cgtcatagta acaccaaaat tcttccaaac agtcatgatg
    37441 gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt gagggatcgg caagccgact
    37501 tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa gcaacataga aaaaataatc
    37561 gcaattgacg tcaatgaaca aataactgga atgtaaaatg tcgaacgaca aaaacgattg
    37621 aatctggtat ttttagataa tgctactgct gctaataaac caacgataat ttgtgttggc
    37681 gtttcgagta cggtgaaata taaagaatga atcaacgaat taactgctcg ttcatcacta
    37741 aatatacgtg tgaaattttc aaaacttaca aaatttcttg atgtaaagaa aatattcaaa
    37801 tctgtaaaac taatagcaaa tgttcctaaa agcggtatga cagtaaataa taataaaact
    37861 aaaaaagatg gagttaaaaa caaatacgca gcacgatcag gacgattcca atatttggtg
    37921 aatccgttca tatagtccac ctcatctttc attttgataa aatatacaat cttggtttaa
    37981 acgaatctga ataagatatc cgaacaatcg ctgtgatttt tgcaataaca cctatctgca
    38041 aatactaggt cgatcattca gatatctgta tcttgccaac agtctatcct gtataaagtt
    38101 ctgtactatt tttgcagtaa ttgattgatg gcatcatctg catcgttcat gagtgtctca
    38161 ggatcttctc ctgctaataa tttttcaatc agtggattga tgatatcgtt attgatcgca
    38221 gagatttggg taaaacctgg taaatatggc tctgcatagt ccatttgttt cgataattca
    38281 gatacaatag gatcattttt tacttcttca tcttttgcta ctgaatccaa gtaggcagga
    38341 aaaccatttt cgatactcca ttttttacca atttcagttg tattccaata tttcaaaaac
    38401 tcatagacag cttctttttt attattatct gtacttgccg gaatggcata tcctacgcca
    38461 tctaaaatag ctgttttttt gtctgaatca ctcccttgtg gtaaggtagt cacattataa
    38521 tcaatctcgt ttgctttcaa tccgttattt aaccatggcc cattgatttc aatggctagt
    38581 tgaccagcat tcattaaatt gtccatttcg gcgccagaaa tcgattctgg accagtcttg
    38641 tctacatgga tcattttttg gatcgctttt aacgttttaa ggttctcagg agacgcaaat
    38701 tcagacttag tataatcatc gttcactaat ttaccgccat ttgctaacat ccagttatat
    38761 aacggtgcag ttccatcctt attgaagaca aaacctgata catttttact actatcagtt
    38821 aattttggtg ccatttctgt caattcttcc caagtagtag gtggttgttc agtatctaat
    38881 ccagcttgtt taaacaaatc attattccaa tacatataca tgccttgtac ctgcatgggg
    38941 acaaaatact gtttaccttc tacttcacct aattcaatag ccgtttcttt aaaatcagat
    39001 ttatctactc catcatattc ccagaaatcg tctaatggtt gtaccgctcc gttttctaca
    39061 tactgtgcaa aatctggata atttaatgca ataaagtcag gtgctttttt agaagaaatc
    39121 gctgtaggaa gtttttcatt caaattggcc caagtcatga tatccatttc aattttgatg
    39181 tttttatcat ttgttttatt aaattcatta acgatttctt gcaatatttc accatcagat
    39241 gcagtaaacc cattccaaaa agtcaaagtc acttctttat cagaatcaga ttctgctgaa
    39301 cttccgccac cgcaagctcc taaaatacct gttgcagcta ccaacaaaga gccaattaac
    39361 cattttttac ctttcatttt ttgttcctcc ttcatctgat aaacgacatc tgaccacatt
    39421 ccaagatagc ggtttcaata aacaattgat tttcccatct tcagctttta catttgttga
    39481 atgacttggt tttacttgtt cacttttagg aagattagtt gcttttgtat caaatccact
    39541 catttcagaa aaatcaatca ctccctctaa tgataattca gacaagtgaa cagttagatt
    39601 catttcttct tctccacggt taactgcaaa tatgacaagt tctttgtcat ttattacggc
    39661 tatactttct aaatacggca cttcttcaaa atcttccgtc gcatagtttg ctgaacgaat
    39721 ttgtgggctt aacacctctc cttgtccata cacagcagct tgcataaatg gataatagat
    39781 cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt ggtgcaataa cgtttaccaa
    39841 ttgagcaaga caagcaatct ttactctatc tgcatgtttc aataaagtga ttaatagaca
    39901 gcctaatagt aatgcatctt caaaattata gttatcttct agtaaaggag gagcaatctg
    39961 ccaaggcgct gcttgctcat cttgttccgc tgaatgatac caaatattcc attcatcaaa
    40021 ggacaggttg atctgcttct tactatgttt cttcgccttg actgtatcgc acatcgctgt
    40081 cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca agataatgag aaagatcatt
    40141 tttttggttg ccataatatt gatgcagaga caaataatct atttgatcgt acgcttgatc
    40201 aagaactgtt aactcccaat caccaaatgt aggcatttgc cgattcgaac ttccgcataa
    40261 aaccacctca attgaatcat ctacgagttt catcgcctta gccgtttcct ctgctaaccg
    40321 tccatattct tccgctgttt tatgtccaat ctgccaagga ccgtccattt cattccctaa
    40381 acaccatgtc ttaattccaa aagggctctc ttgaccattc gaacgcctta aatcactcca
    40441 ataagttcct ttaggaaaat tacaatattc gactaaatta cgagctgcat ctactccacg
    40501 agtacccaga ttgacagcca tattgatttc ggcccccaca tgctgacacc agttataaaa
    40561 ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa gctaaatcca accttctagg
    40621 tctatttgct atgggcccaa tactgtcttc ccaacgaaat cctgacacaa aatttccacc
    40681 aggatatcta atcatgggaa tgttcaattc ttttaccaag tgaatgacgt cttggcgaaa
    40741 tccactttta ttagctgatg gatgatctgg ctgataaatc ccttcatata ctgctcgtcc
    40801 catatgttca ataaatgaac tgtacaaccg cggatcaact ttgcctacac gcttgacact
    40861 actaatcaac attgtggctt ccataactga cctcctttaa tttatacatg aataatacac
    40921 ctcgttgaaa gcgttgtcaa taatataaat gttaaactta cttatttttt atcgatttta
    40981 attaaccaaa atattaataa tgattaaaac aaataattta atataacttt tattaacaaa
    41041 tatgttaata tataagaaaa accttttaag aggtgatata tatgcaacta caaataaacg
    41101 aagaatcctt accggtatat gaagcattag ctagtaaaac aaggatcaag atcatccagc
    41161 tactttccaa aaaaaagatg aatgtcaaag atttggcaaa agaacttgga gtgagcagtg
    41221 ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa catcatcaaa acagaaaaag
    41281 taggacaaca aaaaatttcg agccttcgtg tcgataaaat cgatattagt tttccagaaa
    41341 aaatattcaa tgcttttgat acgaaagaaa catcgatacc tattggtcac tatacaaatt
    41401 atgcaattga accaacatgc ggtttagcta cgatccatga ttttatcggg aaagtggatg
    41461 aaccacgcta ttttatggat cctcgaagga tggatgcccg cattctctgg tttaccagcg
    41521 gatttgtcga gtatcaggca cctaattttt taaatacaga ggatacattg gagatgttgg
    41581 aagtttctgt ggaaatttct tctgaatttc ctttttcaaa tgacaattgg ccttcagata
    41641 tcactttcag cctaaatggt gtggagctag gaacatggac gagccctgga gattttgctg
    41701 atatccgcgg taaatatacg cctgattggt atccagataa tcttaatcaa tacggcttgc
    41761 tgaagacgat ccgaattacc aaacacttga cgaatatgaa tggagaaccc ttgtcaaaca
    41821 tcacgatcaa cgacatccct aaagagcaag acacttggca tttgcgaatc gaagtgaaag
    41881 acgatgccaa acatgtgggg ggctgtactt tatttggtaa aggattcggt aattacgatc
    41941 aggatatcaa attaaaagtt tactacagct gatatttttc ttttctattt tataaaacaa
    42001 aaacagccaa tccctatgaa gggacggctg tttttgcttt attgttattt ccagaaatca
    42061 tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct tgttccacca tgcttcgatt
    42121 ttttcttgtg cttgagcggg aacttctttt ccttctagat aatcatcaat ttcattatat
    42181 gtgacaccta gtgcgacttc atctgcaatc attggtttcc catcttcaag atctgcagtc
    42241 ggaatttttg tgtataattt ttccggtgca ttcaatgctt gaagcaattg tttcccttgt
    42301 cgtttgtcca aacggaataa tggcaaaatg tcggctccac catcaccaaa tttcgtgaag
    42361 aatccagtaa tgttttctgc agcatgatct gtccctaata cggctcctgc tctttctcca
    42421 gctacggcat attgcgtaat catacgttga cgtgctttga tatttccttt gttaaaatcg
    42481 ctgattccca cacctgcttc gcttaatact tgtacttgtg catcaacagc tggtttgata
    42541 tttactcgca gactaacatc gggctggata aagtcaagag cagcttttgc atcttcttca
    42601 tctgcttgtt caccataagg aagacgaaca gcgataaatt gatagttttc atcttttgtt
    42661 tcttcacgca tttcttccat cgttagttgc gccaaacggc ctgctaaagt agaatcttgg
    42721 ccgccgctga tacctagtac aaaagtttta agaaacggat attttttcaa atatgctttc
    42781 ataaagtcaa tacttttacg tatttcttct tttggatcaa ttgttggttt gacacctaat
    42841 tcattgataa tttctgtctg caggctcatt tattctcctc ctatgcttct gtatgaagtt
    42901 ctgctacgct ttttttcatt cgttccatga tagccatttt atggttccag cattcagtag
    42961 acagatcgac tgggtatttt tgcggattca agtctcgttt atactcttcc catagtgaat
    43021 ctagattttc ttttgtatat tctttgattt cgtttagtgc aggtaattcg taaactcttt
    43081 ttccttcgat aaagatatct tgtaaaactg ggcgagctgt aaagtctcga acagttttat
    43141 tgataaacgt atgcactgga tggaacatga agatagcttc ttcttctcga gggtcttcat
    43201 cccataatgt cacgtagtct ccttcagatt tcccatcaaa atttcgagtg atgcgccaca
    43261 cttgtttttt ccctggagta gttacttttt ctgcattgct tgataacttg atcgtatcca
    43321 tcatttttcc ttcatcatct tcgatcgata ctagcttgaa tactgcacca agggctggct
    43381 gatcataggc tgtgatcaat tttgtcccaa caccccatac atcaatttta gctttttgca
    43441 tctttaaatt caaaatcgtt gcttcatcca agtcattcga agcatagatt tttgcttccg
    43501 taaatccggc tgcatctaat tgttcccgaa cacgcttaga tatataggcc atatctccac
    43561 tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa ttctttcgcc acgcgaatcg
    43621 cacttggcac acctgatttc aacgtatcat aagtgtcgac taagaacacg cagtctttat
    43681 gggttttagc gtaggccata aatgcttcat aatcatttcc ataagattgg actaacgaat
    43741 gagcatgggt cccgctagct ggtatcccaa atatcttccc agcacgtaca ttgcttgttg
    43801 catctgctcc gccgatatat gccgctctag ttccccagac tgctgcatcc agttcttgtg
    43861 cccgtctagt tccaaattcc aatagtggat cttctccgat gacagatttg atacgagctg
    43921 cttttgtcgc gatcaacgtt tggaaattga ccatattcaa cagtgctgtt tctactaatt
    43981 ggcagtgcgc taaaggccct tccacttgga tcaatggttc atttgcaaaa actagttctc
    44041 cttcacgagc agaacgaact gttgctttaa attcaaaatt ttgtaaatac tccaaaaaac
    44101 cttcaggata gacttctaat ccacgcaagt aagcaatatc ggaatcattg aacgtcagat
    44161 tctctaaata attgactaac cgttccaatc ctgcaaaaac cgcgtatccg tggttaaaag
    44221 gcatttctcg aaagtaacat tcaaaaacag catgtaaatc agctcgacct aattcccagt
    44281 atgtctgcat catattgatt tgatatagat ccgtatgcag cgtcaaactg tcgtctgggt
    44341 aagtattatt catcttgtat tactcctctt ggcaagtttt catttcctac ccattataac
    44401 aaaaaaaatt aataggaaaa gaaatcttcc attttttctg aaaaagcaca tgttttacgg
    44461 atatttcttg ctaaaaatga actccatctg ctaaaactat cctttcttat tggtatttaa
    44521 cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag aaattagcgt
    44581 tttctatttt tcttacttga taaaataagt agattttcct actacaatcg tagccgttaa
    44641 gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa tggcaaaagt
    44701 ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa acggatataa
    44761 cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac cttgcgttga
    44821 aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag aaagcagctg
    44881 tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt tttttattgt
    44941 atacattaga tatggagcag gttaccataa agatgattat tagtcgttat taaatagaat
    45001 ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg aattgtattc
    45061 cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct cgtgtcattt
    45121 ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc ttcgtattga
    45181 tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt cgatctaacg
    45241 aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact actttaggat
    45301 aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc atctcattcc
    45361 tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct ttcctagcat
    45421 taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt atcacttaat
    45481 agtataacat aatttcatga gaaaaaaata caattttttc ttgaccatct cgttacgtaa
    45541 aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa agaaaatgtc
    45601 agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg gactgctaaa
    45661 accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag tcgatcgtct
    45721 gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc aagaggtctt
    45781 agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa tgttgctgga
    45841 aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg atatgtacga
    45901 aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac aaaagttgaa
    45961 agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa aaaaggttac
    46021 agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa tgcaaactgt
    46081 tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag atgaaagctt
    46141 agcagaccgt atcttccaag ctcataagaa gtggttatct tacagctggc ctgaatatca
    46201 gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt tcactgcata
    46261 ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta tccaaaaaaa
    46321 ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta ataacaaaac
    46381 agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat tctttcacaa
    46441 gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg tattttcact
    46501 ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc ttccagatgg
    46561 tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg tccaatcacc
    46621 actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat atccgaattt
    46681 gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt tcctttctct
    46741 gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag ttgatgacat
    46801 agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa cgtgatgccc
    46861 aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga ttgttctcga
    46921 tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac tagaggtcca
    46981 tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat gatcaatcca
    47041 tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt ctgatagccc
    47101 gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat cccactgatc
    47161 aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct tttcgttttt
    47221 ccataactta actgtctggc atttgcatta gggatataat cgacttcttt catgaccgca
    47281 agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac acgtgaaata
    47341 gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac tgctgcttcg
    47401 ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg cattcaaaaa
    47461 actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa tatatatctt
    47521 ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta ttcaaccctt
    47581 gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa aaataattgt
    47641 tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct tttgatactt
    47701 tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa agaaaagttt
    47761 tattcctctt tcattattac caaagtctct tattttaaat tgaatacatt ataatgatac
    47821 tgtaaatata tctttattat tagtgaatgg atatttactt ttaatagata aaatattata
    47881 actattaaaa ataactattt tcattataat tacacacaaa aaagagcgtg agacaaaagt
    47941 aaagaacact tttgtctcac gctctaaaca cgtataaaca gcggaagcag aagcaactcc
    48001 ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa tttccgtgaa tttcttctta
    48061 tttcttggag ttaaacgctt ctgttccagc ctctttttat ctgtctttca ctaatgatca
    48121 gttgtagata agcaagttgc tttttcttac atcataccgc ccatcattga tggatccatt
    48181 gcaggagctg ctggcgctgc tggttctggt ttatctgcaa cgactgcttc tgtcgttaat
    48241 aacaaggcag aaacggaagc ggcattttgc aaggcagaac gtgttacttt agttgggtct
    48301 acgatacctg cttcaaccat gtttacccat tctccagtag cagcattgaa accagtgcct
    48361 agttccacgt tcttcaattt atcaacaatc actgatcctt cataaccagc gttttcagcg
    48421 atttgacgaa ttggttcttc taaagcacga acaacgatct tgatacctgt tgccacgtct
    48481 ccttctgctt ctacagcaga aactttgctg ataacgttca ctagagctgt accaccacct
    48541 gagaccattc cttcttctac tgcggcacgt gtagcattca atgcatcttc aatgcgtaat
    48601 tttaattctt tcaattctgt ttcagtagct gcaccaactt taacaacagc tacaccacct
    48661 gctaatttag ctaagcgttc ttgtaatttt tcgcggtcaa aatcagaagt tgtttcagca
    48721 atttggtttt tgatcaattg cacacgagct tcgattgctt ctttttctcc agaaccttct
    48781 acgatcgttg tattgtcttt atctacaaca actttagaag catttcctaa gttttcgatt
    48841 gttgcatctt tcaattctaa acctagatcg tcagtaatta ctgtaccacc tgtcaaaata
    48901 gcgatatctt caagcattgc tttacgacga tcgccgaaac caggagcttt tacagctact
    48961 acgttgaatg ttccacggat tttgttcaat acaagagttg gcaatgcttc accatctaca
    49021 tcgtcagcaa taatcaacaa tggacgtgat tgttgcaaga tttgttctaa caatggcaga
    49081 atatcttgga tgttagagat ttttttgtct gtgatcaaga tatatgggtt ttctagaaca
    49141 gcttccattt tgtcgttatc tgttaccata tattgtgata agtaaccacg atcaaactgc
    49201 ataccttcta caacatctag ttctgtttcg atcccttttg attcttcgat tgtgatgaca
    49261 ccgtcgtttc caactttttc cattgcatca gcaatcaaat gtccaacttt atcagaaccc
    49321 gaagaaacag ctgctacttg tgcaatggct tcttttgaat caacaacagt tgagatatta
    49381 tgcaattctt cgactgctgc ttttgttgcc aattcgatcc cgcgacgaat gcctagagga
    49441 ttagcacctg cagttacgtt ttttaaacct tcgcgaacga ttgcttgtgt cagaacagtt
    49501 gctgtcgttg taccgtctcc tgcaatatca tttgtttttg atgctacttc tgatacaagt
    49561 tttgcaccca tgttttcaaa atgatcttct aattcgattt cttttgcgat cgtcacacca
    49621 tcatttgtga tcaatggaga gccgtaagat ttttctagta cgacatttcg gcctttaggt
    49681 cctaatgtta ctttcactgt atctgctaat ttatctaccc cgcgtaacat agctgcgcgt
    49741 gcgtcttctg caaatttcaa ttcttttgcc atgattgatt cacctcattg attatttttg
    49801 attatttttt tatcttccaa atttactcaa caatcgccat gatatctttt cctgcgacga
    49861 tcagatattc ttttccttcg tattttactt ctgtaccggc gtatttttcg aacataactt
    49921 ggtcgcctgc ttttacagcg gcaggaactt tttcaccgtt ttctaacaaa cggccttcac
    49981 cgactgcaac aactgtccca gtttggggtt tctctttagc agcagaagct aaaacgattc
    50041 ctccgacagt tttttcttct tcttttgcga cttcgataat gacgcgatca cctaatggtt
    50101 ttaacacgat aaatccctcc gataactctt tttttaatct gttagcactc ttaaccttcg
    50161 agtgctaacc tacattttta ataatactca ttctccctct tttttgcaag cttttaaatc
    50221 aaagaaaaga aattttattc tgacagctgc ttattgaaat tatgatataa ttgaaatcaa
    50281 tcgatataag cttgccttgc atgcttgtta tcccttaaaa ggaggaatta catgtcttta
    50341 aaaaaatata gcttcctcag cattttttgc tatggtctaa tttttatctc acccttgatc
    50401 ctagcttcga taggacttgt aaaatctacg tctgaattga tcacagcaac tgcagtcatg
    50461 tatatactag gcgccgttgt cctagctatt ttttatttta agcaacgtga acccttagcg
    50521 atcgaatcag cggtaaaaaa agcttctgct ccaaagattg ttatttatgg attggttgga
    50581 atctttgtcg cattgattct gcaaagcatc gctgtcatgt tagaatctgt attgtttggc
    50641 aaagcaaccc cttccgagaa tacacaaaat atcatccaga tgatcatgga agcacccgct
    50701 tttattttgg cgacaactgt tgctggaccc atcatggaag agttcgtatt ccgccgcagt
    50761 atactgggga tcatcagtcg atattcaaat ttttgggtcg gagcagtgat cagctctttg
    50821 ctgtttgcgt ttgctcataa tgatggtcat ctgctaatct actttttcct aggtttcttc
    50881 ttcagcctag agtacaaagc aactggtcgg atctggacat cgatgatcac acacgtagga
    50941 atgaacacat tagttgttct tgttcaatta gctgtccaaa aagggctagt ataaacatga
    51001 ctataaatac tcctgtcatc ctagattggt gattttccca atctaaagcg acaggagtat
    51061 ttttgttttt tagtcgttca tcttttctgc tgctccatca ttttttgtct gtctttcctt
    51121 tttgcagttt gcagaatcta tttttttcaa aaatgggaaa taaatgaaaa aactaattga
    51181 caacacgata ccttgtaaaa tcgctgcagg cactccgcct actaagaaac ccgagaggat
    51241 cggcggtgtt gtccaaggga cttggactgc tgtaaataaa ggaacgaatc caaaatataa
    51301 agcggaatag gtaatcaatc ctgaaacgac cggtgcgaaa ataaaaggaa cagccatcat
    51361 cgggttcatc acgatcggtg tggaaaaaat aattgattca ttgatgttga aaaaggctgg
    51421 taaaagagca agatttccta attctctgta tttcttcgat ttcgcaaaaa ccgtcataaa
    51481 aaatacagcc cctaacgtca atccagcacc cgtaaccgtc atgaactgat cgagaaattg
    51541 ctgagtaacg atatgtccgc catttgccac cgtcaactct tgtcctgagg ctaagatttc
    51601 tgcatttgct aatgaattcg cttgcaagac gggactaacc aacccattga ccatcgcaga
    51661 tccatgaaga ccaaataacc ataaaaacgg aacaaaaaaa cctaataaaa gcacaccaaa
    51721 caacgtgtct atgaacgatt gaatgggaag ctggacatat gtccatactc cttgaacaac
    51781 agtcattctg aacaaatgtt gaaaaaccgc tgaaccgata agcgtaaaag tgactataac
    51841 aatgattgga atcaatgaag taaaagactg ccaaacattt tcaggtatct tctctctttg
    51901 cttccaccct ttcgcaagtc ttactaaacg tgcatagccc catccgatga tgagcccagt
    51961 tgtaactgct cctagtatcc cttcagcacc tgtccagaca gtatctatct gagtatttag
    52021 tggctgaata agcagtagat aagcagacaa ggctgttatg ccgactggta agcgatcgat
    52081 gtcttcttct tttgcataag cgtatgcaat ggcaaaaacg atccaaaagg ctatcacatc
    52141 aaatgtagcc tggtatactt gtgtaaaata catagaaaat cctgttttgt ctatccactg
    52201 agcgatgtaa tccacaggaa aatgagcaaa taagaaaaac agcgagccaa caataattat
    52261 tgggatcgtg taagtcattc cattacgcaa agcagttaag ggtctaagat tcacaacaga
    52321 tgttatcatt ggtaaaaagg cacgatctaa ccactctttc atttcttatc tccttcaact
    52381 tctattttta tcaatcagtt ttcttaattt aactataccc aatgatcacg aaccatgaaa
    52441 ggaatgcgca ctgagctttt caaaaatcgg attctttgac gaatattcag caactgctta
    52501 caatggacag gaataaaaaa ggaggttttt tatgaaaatt gcactcattg gagctactgg
    52561 ccatgcaggt tcatttattt tagacgaagc attgtccaga gatttagacg tgacggcaat
    52621 cgtgcgtcat ccagaaaaat tgccatcaga tgttccgttt atacaaaagg atttatttga
    52681 gttgacaact actgatcttg catcttttga tgttgtcgtt gatgctttca acgctcccaa
    52741 aggaaaagaa gaaatgcatc aaacttcttt gtcccatttg attgagatgc ttgaaggaac
    52801 cgatacacgc ttaattgtcg ttgggggtgc ctcttcgtta ttcttagata cagaaaagac
    52861 gactcgtatg attgaccaag tacctaaaaa tgcacccttc tatccaacag ctttcaatat
    52921 gtacgaagga ttgttaaaat tgaaacaatc taaaaatcta cattggactt atatcagtcc
    52981 agcttcttta tttgatccac atggcaaacg tacaggtact tatttgttaa gtgatgatta
    53041 tttacaaaga aacgctgcta gcgacagtac gatcagcatg gcagattacg cgattgcatt
    53101 agtagatgag atactggatg agaaacatga aaagcagcac atcagtgtag tcagtcgata
    53161 atacggttat ttttttaata tgctaaaaaa ataaaaatag aagataaaac caaccctgat
    53221 tgattttatc ttctgctttt tatattttta ttgattgaac tgtttaaatc gaaaatagct
    53281 cagttgcttc atctggatct gtatcataga tttcaaaatc atgtccaacg ccaaaatctt
    53341 tttctttcaa aacattttcc attgttaaat gagcaagttc tttcatattg ttttccttgc
    53401 taagatggcc aagatagatt cgtttggtcc gattaccgat cacatcagtc atgacaagtg
    53461 caccgtcttc gttggaaaga tgtcctctat ctcctaaaat acgctgtttt aaattccacg
    53521 gataaggtcc catgcgtaac atctcaagat catgattact ttccatcaaa taaccatcgg
    53581 cattttcaat gacacctcgc atataatcac tgcaatatcc ggtgtccgtc aacatgacga
    53641 atgaccgatt ttctttatgg aaacgataaa attgcggagc cgctgcgtca tgggagacgc
    53701 cgaaactctc aatatcgata tcgccgaatg ttctgacttt ccccatctca aatagatgtt
    53761 tttgttccac tggtacattt ccaatcagtg gttccatcgc ttcccaagtc tgctgattgg
    53821 caaaaacatc caaatgatac ttacgagcca agacacccac gccatgtata tgatctcgat
    53881 gttcatgcgt cacaagtatt ccatctaatt ggtccggtgt ccgatcaatt tgagccatca
    53941 aggaagtgat ttttttaccg ctaagtcctg catcgaccaa aagccgtttt ttttcacttt
    54001 cgatataaag tgaattaccc gaacttccac ttgctaggat actgatttta aaagctggtt
    54061 tttcttcagc catttgactc ctacttccct tcttactttg taaacttttt tcattatact
    54121 cgctttttta ttgatcttcc acttttggta cagtattgtt tgtcacgatc gtattgctga
    54181 aagcgttgac gctctcaatt tggaccactt tatcgcttgt ttcgatcgct acatgccaaa
    54241 caggaacata cacatttttt tcccggactt tcagaataca ggaataagct agctgacgcc
    54301 ataaaatttt tgatcctctt ggtattttgt tattgatgta caatgtattg attgcttcct
    54361 cttccgtata cagttccgtc ttttcgcgca gctgctcgat atcagataaa tgcgtctgca
    54421 tgtatttcgt gatgctataa tcattatcgt ttttttctaa agaaagaacg atttttgctg
    54481 tatcatcatt gaatgggatg ccttcatact cttggcttgc ttggatttct agtggttctt
    54541 cttctaaatg agaaaagttt ttcgtgtaca cataatcatt accgaataaa atattgtctt
    54601 tgttagataa aaaatcttcc aaggaactac taattttatc tggatcgatc acgttctttt
    54661 cagcatctga actggataaa gatttcgtca aaacatcacc gtctaaaaac acaccacttg
    54721 ccagcaagcc gcttttacca gccttgcgat aattcgctaa tgaagtgcta agattggtct
    54781 gctccgcgct aagataatag ccttgcttgt tctcagacga aatcttcccc gtgtaagtga
    54841 tatcgtcgct agccagacgt tgttggattg attcagtctg attagagcga atgacattgt
    54901 tttcttggta taccgcgtca tgataactgc tgaataaaaa cacattcaac cctaaaaaag
    54961 cgacgaaaaa tatccattcg atttttttga aatccaatta attcgcctcc aatccaggta
    55021 atttttccag aaggtcgtta gctgaatacc agttatcttt atatttcaca taccattcag
    55081 gtaagagatc aacgacacga ttcgtttctt tgacattttg ccatgtatag ccaacgatca
    55141 ctgaaccaat cttgttgcta tctgctccct tagcttttaa ctgctggatg atttcattcg
    55201 tacttgcaag ttctacttct tcgtctgaag gtatcggcac ttgaatcgta ttcaaactgg
    55261 tttggatatt cacttgcaca gaaccattcg tttcaccgcc aacttccaca ccaatctgtc
    55321 ctctgctgtc tgtcccaaaa accgggaagc cttccacgaa tatccgataa tcgatcgtat
    55381 tgtcactgcg gtcaaaatat ctgaggttcc ctaaagaact gcctaatttg ctaatatagg
    55441 ggaaactttc agaaaataca ctagcatttt cttttttctc tggcagctct ccacgaaaat
    55501 caactacttg cgtttccgaa tggatagaca ttgtttcatt cccaccgtaa aaaatcaatt
    55561 cattgctttc atcattattt ttcacgctat ctggtttctg gaaaaaagca tttctaaaaa
    55621 gtgtataggg ttgctgtgac aggatataac tatacttctt cagtttgatc gaatgtttcg
    55681 tagcatattg cgtgtcgatc gtcgatggtt ctgttgtcat ttctgtccat tttgtgtctt
    55741 ttgctgttaa ttctttattg atatctgtcc aattcaacga aatatttgct tcatatacga
    55801 gatggttcga ataattcaaa aatcggattt ttttatttcc ttgatcgaat tgtattctag
    55861 taaaatacat attttcgctt tcttttgcca agttcgacac atctaaatct aaatcaaatg
    55921 cttccttgta ttctcttagc aaaaaaggcg cattataggc caactcaaaa ccagagttca
    55981 gatttttcac ctgttcaaac tgtgatttgt ctcctgaaac tacttcagaa agctttccaa
    56041 atcttgcttt atcgatgatc aattgcaatc ggtacacgag attttcactg ttggtttctt
    56101 ggatcattcc cgaatggtgc catgtaacat gaagcggaag aaagacttcg gaagtttttc
    56161 gattgctttg agactcgatc atttgagtgt ttgtctcatc tacgttgatt gaactttttc
    56221 ctgctgggct cagccagatt gcataagaga agtatatgct taacgcaacc atcaaaatga
    56281 gtcctatacg aacgactttt tcagagattc tcattcccac cattcctcct cataaggttc
    56341 atatggcagc gagatataaa aagtcgaccc ttttccttcc ttgctctctg cccaaatagc
    56401 gcctcgatgc gccttaatca cttctctaga aatagctaat ccaagtcctg tcccaccttg
    56461 tgctcttgca cgtgctttat ctactcgata gaaccgctcg aacactcggt tcagatcttt
    56521 tttaggaatc cccaatcctt gatcggtgat acttaaaatc acgttattat gcgtttctaa
    56581 taagcgacaa gtaatcgttc ccccatctgg tgaatacttg atcgcgttat tcatgatatt
    56641 atcgactact tggatgattt tatctgtatc tacttcgacc caaagatctc tttgcgtaaa
    56701 ttcacgacgg atcttataat ttttttcctg atttcccacc atcatatcaa accggtcaag
    56761 aacaaaattc actaactcgt tgaaattgac atattctaat tgaagctggg tattacctgt
    56821 atccatgcga gatagattta agaggtcatt gatcattcga atcattcggt ctgtttcatc
    56881 tagcgttacc ttcaaaaaat ttggtgctac ttcctcgtct ttccacgcac cttcgctcaa
    56941 tgcttcgata taactacgca tacttgtcaa cggtgtacga agttcatggg aaacattaga
    57001 gacgaattca cgacgttccc gttctgtttt ttcttgttct gtcacatcat gcaagacagc
    57061 tactaatccg ctgataaaac ctgattcgcg acggatcata gaaaagtcta ctcgcaagat
    57121 catctgatca ctttccaatg tactggttga acggtcaagt accatttcgt ttggatcttc
    57181 taataatttc cgcaacgtat attcttcttc gatatctaaa agtgtcaaaa tcgattgtcc
    57241 gattgcttcc tcgtttttga tatctagcaa ggaaacagcc atatcattga tcgtaatgac
    57301 tttacccctg cgatctgtcg caatgacgcc gtctgtcata tgggatagca cgctatccaa
    57361 acgattgcgt tcagcttcca ttgtatcttg agcttcttca atacgttcag ctaactgatt
    57421 gaaggtatca gccagctgtc caagttcatc ttgtccatgc actttgactt tccgactgta
    57481 gtctcctcta gcgattcgaa tggcttgttc tctcatttca ccgatcggct gtgtaatcga
    57541 acgagcaact aaaacagata cgatcattga aataaatgcc gcaattagtg aggcagtaaa
    57601 gaaaatgact gctgtgttat tgatttccga atatttttgt tcgatattac ttttgacgta
    57661 aagagccccg ataacggcat cgccggtcgg ggacaggatc ggttgaacat tgatattcac
    57721 ccgtttatcg tcgttatcta aagcaacata acgtttagtc gtgaaatcat tgatatcaac
    57781 ataatcattt ttctttccca cagcgctttg ttcattgaca tcggttgttc ctcggatgat
    57841 ccctttttcg tcaacgaccc gtatttcgat gatatcgcta gtcgcactgt tttcaataaa
    57901 tcttttgata ttcgcattta cctcttcatc atctgcatta tcttttcgat tcaattccgg
    57961 gctgattgtt gttgctaatg attccaccgt ttgattcata tctttaataa atgtattgat
    58021 cgtggaacgt tctaaaccac gtataaagta ggcaccaata atttcgatcg agatcaataa
    58081 aatcagaata aaggtaatag cgatcttaaa attgactgat cgaaaaaagc ggacttttct
    58141 tttcatccaa ttactcctgt tctgggttac gcaaatagta accgacacca cgacgtgtca
    58201 ctaaataatt tggatggctt ggattatctt cgatcttttc acgcaaacga cgtacagtta
    58261 catccactgt tcgtacatct ccaaaataat cataacccca aaccgtttgt aataaatgtt
    58321 ctcttgtcat cacttgtcca aggtgcttcg ccaagtaaaa gagtagttcg aattcacgat
    58381 gtgttaactc gattgtttct cctcgtttcg tcaccatgta agcttctgga tggatcgtca
    58441 aatcaccaat cgtcaattcc gcaggtgctg cctcttctgg ttccttcgca gccgtggcac
    58501 cgcgacgaag atttgctttg acacgtgcaa ctaattctcg gttggaaaat ggcttggtca
    58561 cgtaatcatc ggcgcctaac tctaagccta ggactttatc aatttcagaa tctttggctg
    58621 ttaccataat gatcggcata tcataagttt tgcgtacctc acgagcaact tctaagccgt
    58681 ccattttagg tagcatcaaa tcaagtaaaa tcaaatcagg ttctacctca gcaacttttt
    58741 ccaaggcttc ttccccatca tatgcagtat aaacgtcata gccttctttg gctaaattga
    58801 atttgacgat atccgaaatc ggcttctcat catcgacaac taaaactttt ttcataaaga
    58861 gccacctctt atatattatt gtcatacctt ctcattatac cgtatttctg cttttgtttc
    58921 atctttgcct ataaaacaaa agagaaaatt aaaatgaaag tgcaaaatac tttattattt
    58981 tttgttctaa cttcaataaa aaaggaaaac actgattttc acctcagtgt ttcctttttt
    59041 tgatttatcc ttctaaaact tgtacaccga cacccaataa tcccggacca gtgtggacta
    59101 ctaatgccgg agaaatctgg ccaaagtaga tttggtttgc ttgtgggaac tctcttttaa
    59161 gtctcgcttc catttcaatt gcttcttctt tcgcatcccc atgggcaaca gctaagttga
    59221 agacctttgc attaccgatt ttttctttga cgtaagcaac cgttttatcc aaactttttt
    59281 tacgtcccct agcttttcct actgtgtaat aaatcccttc ttcattacat gagatgattg
    59341 ggttaagctt taaagcggtt cctacaatag aagcaactaa accgatccgt ccaccttttt
    59401 gaagatattc caatgtcgca acattaaaga aaacttttgt tctttcgact aactcattca
    59461 gattactgat cagttgatcc cattctactc cttcttccag ccattttgct gcttggatcg
    59521 cagaaaaacc tgcaccaata ccaatatttt tcgtatctag tacatatgct tccaattctt
    59581 cttgctgttg tgcaattaaa cgaaccacat tatatgtccc gctcaatcca ctggaaattg
    59641 ttaccgcaag taccttttca taaccgtctt ttttgatttg ctcaaagatt tctgtgatcg
    59701 actcaccatc cggcaaagat gtaccaggga tttcctctgg caaacgttga tagatctctt
    59761 caggggtaat gtcaatttta tctgtatata cctgatcttt ataaataatt ttcaatggga
    59821 tcatatacat ctcgtatttt tttatcaatt cttctggaac atccgtacct gaatctacta
    59881 aaattgcgat tttattgttt ttcatttgtt ttctcctctt ttaaatattc ggtttctaga
    59941 acaatcgttt tttccgcaag aagtttcatt gcaaacgata aagttgccgc tcttacagca
    60001 atatattcta caggggttgc ctggtcgaat ttttgcggat gagatttacc ttgtgctaat
    60061 tggctgacca tccacacagc tgcttcttgt tcatcgcaaa atagattata ggcttcacga
    60121 attcctactg tttttccttg gaacaatatc ccttctttga tttcaggaat agtcaatact
    60181 tgtttcaacg tagtgattgc aagtaaaaat gctacgtgtt ctttgttata tcttttttta
    60241 actggtgctg gtatcaaacc tagtttaacg taattgttta ccatagaaga ggtaagcaat
    60301 ggatgctttt ctccttgtat cacaggtgaa agataacgat caactaacgt tctgacttgg
    60361 tccatataaa gttctaagtc agggagttct tcccagtgag gcagacggaa tgtttctagt
    60421 tccttccccc actccattaa ttgctgacgc attttttcca tcataaccgc tccttttttc
    60481 atttcataaa catgatatca gacttatgat aaacattcaa tctagttgct ttaactaggt
    60541 gatttgatta gcgggattcc atgaaaacaa aaaacaagaa ggctaagcct tcttgtcttt
    60601 tatcgttaag aacaggagac ccgccgggga gacggcggga aaggagtaaa aaatgaaaaa
    60661 agtgttaggg tttgttctct tgcaagaact ttgtttgttg gtatgcttat ataatagcga
    60721 gcaaatgtga ataatctatg aatgatttcc tataaggaag taaagatttt tctgattttc
    60781 tttaaatgta gtcttgcatt ttaaaattct tagtgctact attattaagt actttttgta
    60841 cgggccgtta gctcagttgg tagagcagct gactcttaat cagcgggtcg cgggttcgag
    60901 cccctcacgg cccattgggt gccaaaccca cggaacaggt aaatgttggt gcgaaagcat
    60961 ttcgaaaaca tttacgctgt tcttttttta tttttcaaaa taactagggc tttttatttt
    61021 taactcaaag tccactgtag ccctttggac tttgagttaa aaaagaagca taattatgaa
    61081 cagtaaagtt tctaatataa caataattcg agaagctttg caaaatcatg ctgccaatga
    61141 actctctaaa catataggat taaatctaag tactataaaa ggttaaaaag tgaagaatat
    61201 ttaatagaaa aactaaattt acttgctgct ttcccctttt ccattaatac aacaatcaga
    61261 gagtgtcttt ttttcgaaaa aattagaata aataaaagac acgtagtttt tttatctaca
    61321 tgtcttccat cgactacaag ttatcatttc tcaaaacgta tttagctttt aattgattaa
    61381 tcgtttcacg ttgtctacac attgcctaat taagattttc ttattagaag catcaatccc
    61441 gcaataatac ctaaaactcc aggaattacc cacaataatg ttaaaagaaa aaatggaata
    61501 atagataaaa tgagtaaact aataccatag accactgttt tttgattttt tcttactaaa
    61561 aaacctatta tgaaaatcat tatcgaaaaa actagggtaa ttcctccaaa taaaacaaca
    61621 ttcccgctta gattgactaa catatctata tccttactgg ttagttctgt agtatttaaa
    61681 gtagttgaat caatagaaga tagaaacata ccacccatca atgctaaaag actacataaa
    61741 atccctgtta ttccactaat taaaatcaaa ataaactctg tttttctctt attcatttaa
    61801 ttctctccag attaagtgtt ttagaattag aaattttttg accatagtta atagcttcta
    61861 atgaacgaat aacaaccact gacgtgttag aaaaataatc cccaatatgt tgtttcttgg
    61921 gcgtgaaagc agccaccaaa tacccaattt gaaatacaaa attttttaat ataaacctac
    61981 atatacactc acgaataaag atagtttgcc aacttagctc tttttcctca aagctcacta
    62041 ctcttatccc aaaaatcatt ttcccaattg tttgtcctga attcagctta gtcaaaagta
    62101 caaaataggt taaataaata gctaatgacg ctataccata aaaagtaata atggatttgt
    62161 cagattccag tcccatgatt ttgtaaaaga gacctatagt agcatgggtg atgatgctaa
    62221 tacaaattaa atcaataaga aaagcaaaaa atcgtaacca aaatcccgca aatacataat
    62281 taggcaaagt ttcaatgcca ctcttcttat tcattttata gccctccata gtaatacatt
    62341 ggttttggag tatcagaaaa tccaagtttt tcaaaaactg aaattacacg atctgatgta
    62401 gttgccttta gcccttgcca ttcagcaatc ttattaccta accaagaaga agcaaagcca
    62461 gttgtgtcat ttttatattc tataaccgtc gcatcagtta atttcttctc tttttttagg
    62521 gaatctaatg catcttcaga gtaaccaatt tgatccacca acccattttc tactgcttgg
    62581 ttaccatcat aaattcttcc atccgcaagt tctttcactg cattagtatc catatttctt
    62641 ccttttgcta caacattgac aaaacgatca taagcactca tcacaaattc ctgtataact
    62701 ttattctctt cctctgaagg tttgtgttga ggtgacatca tatcttttaa tgcaccactt
    62761 ttatacgtcg catcagttac gccaagtttt tctaataagc cagaataatt taaaccagaa
    62821 ataatgacac caattgatcc agttgttgtt tcttccgtag caaatatttt atctgaacca
    62881 gcagaaatgt agtaccctcc acttgccgct gtatttttaa atgcagtata gataggaata
    62941 tctagctttt taatttttgc catttcttta gcaatctcag cactttcata tattcctccc
    63001 ccaggtgaat ttacttctaa taacactccc ttaaccgctt tgtcttcttg aattttcttt
    63061 agctgtgtta aaaaattctg atggttatat tgttccctac taaaaagatt gctttcacct
    63121 gtatctgcaa taactccatt gacggatagt ttgacaatct ttttatttga tgcgccttct
    63181 tctaacgttt cttctgtgat ttcgttagat ccgtaaaaaa tcttatttat tcctgttagg
    63241 gttgtatttg atgcttcctc tttatctttt cctggaatta gcgcgacgat aatggatagt
    63301 aacaataatc cgcacgctac tcccacagca atccatcttc ttttattcat ataaactcct
    63361 cctatattct tagtaaaaat aattattttt tatttagaaa cacatcgaca atttgttgcc
    63421 aatatgtttt ttctcctaca attaaagaca actctccagt taatccataa cgaaacggaa
    63481 acgattgagt tgtttttaac gttcctttca ctgcataaaa aacacctttc tcagtggcag
    63541 tagaattttc tgaaatttca tctaaagttc cgtcgatcgt aatcggagcc ccaccttttt
    63601 gatccaattt aaaatgaacc ttcatccctt ctttaatata ggtactttca ttcgcaggta
    63661 aaagtgctgt aaatttcatt tttgattttt cgtggatcgg ataaatttct gcaagcactg
    63721 ttcctttggc tagttctgct tgtccctttg tttcttcatt taaatgaacc gttccagata
    63781 caggagcttt tagtattcct tttgaaagtt gatcgtccaa acttttgata actgactggt
    63841 tcttctcttg tttttcattt agctctgtaa ctgtttgttt tacagttgtt attgcttgtt
    63901 ctagctcttg ctttcctttt tccgtttgac taattctgtc attctcagaa gtagttggtt
    63961 taactaattt agctttttct gactgatact gttctacttc tttttttagt tgctcaattt
    64021 gttcatttat gcttgctgaa attgtcagtt tcacttgatt tttttgatca tcagaaacat
    64081 tatttagttg ttcttgccaa ttctcatagt ttgccatcat ttcttttgaa aaatctttta
    64141 atgattgatt attactccaa gctacctgta cttgttgcca ttcttgtaat ttactatctg
    64201 aactctcaat ttgtttttct agttgtcttt ttgtcttttc gtatacttct aattgctttt
    64261 gatcatttaa ttcactttgc ttcaaagcgt agcggagtga ttctttttct gacaacatgc
    64321 tctttaattg attactgtag ccaaaactgt catccgttga aaacaaattt tttcctttac
    64381 ttatactttc aataaatagc tgagccatct ttatttgttg agtaatacgt tggttttctt
    64441 gttctatctg agccttttca ttctctatta aagaacaatc caatctaacc aacacatcgt
    64501 ccttatgaac aaattgattt tcttttagga agttttccat tattttagct tctaatggca
    64561 cttgtaactt ctctattttt tcggggttca attgagccgt tgtccgaata acaacctctt
    64621 ttttagcaaa ggttaaaaac aatccaataa tacataaaaa aagaattaca gggtataaca
    64681 cccaacggta gaagctatga tgttgtttag tatagatact agaatgttcg aaccaattat
    64741 tattctgcat aaattgtttc cctctcctag atttcccata atttttgata gataccatcg
    64801 gcaaaacgaa gttcatcgtg cgttcctttt tctaccaaat gcccttcatg gagaactaaa
    64861 atttgatcac atgcttttgc tattggtaaa tgatgcgcaa taaaaataat cgttttttct
    64921 tttaaattga ggagataatt cataattgca tgttctaaca aggtatctaa tccactagtt
    64981 gcttcatcta aaattaatat gtccggattt tttaataaag cccgtgctaa ggctaatcgt
    65041 tgtcgttgcc ctcctgaaag attatttcca ccttcttcca acaatgtctc gtaacgtaat
    65101 ggctgttgat taataaattc atttagttgg actatctcac aaacttctat tatttgttcg
    65161 aaacttggtg ggctatctaa accgaatata agattctcaa tgattgttcc actaaagaaa
    65221 aatgactctt gaggcaaata tgtcactcga tcgcgtaata tctgaaaagg aatgtctaag
    65281 tgattaatgt ttccataatg gatcgatcct tctgatgctt cataaaaacg aacaaataac
    65341 tttgcaagag ttgattttcc agaaccacta actccaacta tagccgtttt actgtgcgaa
    65401 ggaattgtac atgaaatttc gttcaacgtt ggggccttca tgctatacga gaatgaaacg
    65461 ttttttacag tgatatcttg ttgaaataat tgttcagaaa gcatatattc aaaagactgg
    65521 ttcgattttt ctgaatcaat cgcgaaaatt tcatttaaac gattatttgc aacttgagcc
    65581 gtttgcattt tcacttgtaa attaataatg ttttgtaaag gatctgtcaa aaatactagt
    65641 aaagcattgt atgtgataag ttgacctaga ctaattctat tatccataac aaaataagaa
    65701 cctagccata aaattaatgc actgctaatt aattgaatca aatgtttaat tccttgttga
    65761 atattatcaa gcgtaaccgt acgaaatgat tttttcatta atttaacaaa ttcatggtct
    65821 atacggttat atactttctc ttcaccgcta tatgctttaa ttgtttctat tcctttaaga
    65881 ctttctatga tactagaatt tacagctgca ccggctgcca tttcttccct gttcgatttt
    65941 tcatagcttt tcacgaaagc gaatattgca actacataaa aaggcaaaga gcatagagtg
    66001 attaaaaaca acgtactatt ctgaatagct aacgtaaccc caacaagcaa caccatacca
    66061 atatctaaaa atattgataa cgtagcactt gctaaagcat caataatctt actggcatca
    66121 agaaaacgag aaataatttc ccctgatttt ctcgtagcaa aaaaattcat tggtaaagtt
    66181 aacacatgtt tgaaatactg aagcataatt gccatactca ttcgctgtcc taaaataata
    66241 agaagataac tacgactata ttcaaacaat acccgaaata aatagacagc gatcaagcca
    66301 atggaaatta tatttaaagt tgaacgtgct tgattaggga tgaaaaaatc aagaatacct
    66361 tgaaaataat atgagcttcc tatgccaaaa aacgtaatga aaatcgatgc taaaacgatg
    66421 tggaatacta atcctttttg tttccaaatt ataggcaaaa aagaacttaa tccagctact
    66481 ttttctttaa taggaatata agtttctcta ggcataggta ataaaagaat acctgtccat
    66541 tctttttgaa acgcttcaat cgtttttcta ttttttccat tagcgggatc tgcaatccat
    66601 agtacgtttc ccttcgtttt ataaattaca acgtagtgca tatagctgtt atcaaccaat
    66661 atatgtgcaa ttaatggtaa gataagatct ctttctttcc agatctcact atctgcttga
    66721 atagccatac aatcaaaaga caatttttca aatgtctttt tcaatccaaa tgctgaagta
    66781 ccctctaaat cagtcccaga taggtcccgt aatttatgaa tcgggatatc agttttaaaa
    66841 tattttaaaa tcatcgaaag gcaagcaaca ccacaatctt tttcatcttg ttgctgcaca
    66901 aaaggaaatg attttatcac ttatctaaca caactccttc taaaaaattt ttatagaaaa
    66961 attctcaata tagctttaat acagtgaaaa tattgtctca taattccaca aaaactttga
    67021 aaacagttct tgaacactaa ctttttcatt cctgaatcaa atttattttg gaaattttac
    67081 tattcaagaa taaacttgat aagtttcagg aagattttta ctgttaatca aatacgtatg
    67141 ttacagtagc accgtaaaaa atattattta aagaagcgtg tttaaacatg caaaatgtaa
    67201 aggaactaag tattgtggaa atgcacaaac tgttggtgga ggcaataatg cttggggaaa
    67261 attggggcaa gttgtaggtg gacttaccac aggggcagta ggtggtgcag gtttaggaac
    67321 agcaatttgc ggaccagctt gcggtgttgt tggagggtta tatggagctg tcgctggagg
    67381 tgctgcagct ggctgggatg ctcgaaaaaa ataaaaaagg agaataagta gtatgcatat
    67441 taaaaacaca aaaacaacat ttatattatc ttcagaagag ttgaaaaata ttcaaggtgg
    67501 gagtgccgtg ggagttttag gcactacttt ttctggggca acagcaggag taaaactttg
    67561 ttcagccgga ggaccttatg caatagctgc ttgtggtgta ggtggagctt tattaggtgc
    67621 tggattcagc atgtggaccg gtgcgtaaac aattaaaaaa tatcaaacct caagattggt
    67681 ttgtgtcgat tacgaacata ggtatgttct ttatcttttc aaaagtttgg aatggacaga
    67741 actttttact aaagcttctt gtgctagttt ttataattat attaaatttt tatcttttat
    67801 taaagtcaga taaaaattct ttggaaatgt aatggtagag atatttttac aaaaggactt
    67861 gcagcgctgt agttggtgct gcaggaggac ctatttcggc cgttacagga ggtattggta
    67921 atgtacttgt cggatgtgca ttaaagcctg ttcctgcaag ttaaatttat aaaaacagga
    67981 aagtaattat ctcaaatcct atcaagtaca atcataggat tgcacttgac aggacaaatt
    68041 ttttaactaa aaaatgaaaa acactttcta aaaattagta gaatttgcca aatctttcgc
    68101 atcttccacc atacatttaa ttttaattcc cctttatggt acttaataaa aacaagaaaa
    68161 gaactattca tttggccctt ttttggttcc agaccataga aatattcctc cagtaactaa
    68221 tattgctgtt atgcacgtaa ctaataacag ctataaaaat tggtacaact ttaataggat
    68281 tcagaaaaat ttgcactagt gaaggaatct attttattag aaaaatgcaa tctccctaaa
    68341 gaaaaaacca ctaaacagtg ctttctttca acttctcctt aattttttgt gctttcccta
    68401 aagtaaagtt tctggccaac tctgaatcaa aatatagtga acgtttttta aaatctattt
    68461 ctttaacgtt aaagatattt actaaacacg ctcgactaat tcttgttaac ataggatatt
    68521 tcttttcaag ctcactaatt cttccataaa attcgtactg cccatcttta gtacacaatg
    68581 ataatctatg tggtaatttt gaagcttcta caaaataaat gtcatttata tcaaaagtaa
    68641 atgtttgaga acctatggaa aaaacaaatg cttgattcgt aacctgtttt gttgcatcta
    68701 tcctttcttg cgctaataac aataactcaa caatctcgtt tcgatactca tctagtgttt
    68761 gatcttttgt tacaaatcct aacgtttcaa ctcttctttt tattgttacg ggtaacatct
    68821 catcatgagt agttgtaaaa atgatttttg cttgaacatc atattttctg attacttctg
    68881 ctaattctat accgttaact tcatgattta aatcaatatc taagaaatat attccttgct
    68941 ttggtcggaa ttgtttgagg tattttttaa cttctaacgg actttgtgtt ttcaatacta
    69001 tcttaaatac tttatcgtga aaaagaatga aattatcaat aattcgttct aactgattta
    69061 attgtataat ttgatcttca cataatatta ttggataact cataaatcct ccttccctca
    69121 aaacaacaaa ataacttgag tagtaaattg atacggatcg ttttgatagt tcacaaacat
    69181 attgggattc tttttattga tttcctctat actagataaa cctaaccctt gatgtccctt
    69241 ttttgttgtt attccttttt tcattagagt atctatagag agtagtatct ctctacaact
    69301 attcgatatc gaaatttcta tctgtttatc atctcggtaa atagctagag acagttctct
    69361 atcttgactc tctatagatg cttcaattgc attatcgaga ataattccaa gcaccctaat
    69421 acagtcaaat attggaacag gaacttcaat aatagtactt ggacattcga atttacaaca
    69481 aatcttattg ttatttgctt ggtatagctt cgctatcaac aaacttttca aatattcatt
    69541 ttttatattt tttaaataac gatattcaaa cttattggtt agataagaat tagaatagat
    69601 ctctaattct tttatttctt tttccaatat cgaatcatgg ttttgattag cgatttcttt
    69661 taaactcagc aataaatttt tatagtcatg tctaaatctg gcaaattttt cttgatcttt
    69721 ttccagtgaa tcagtatatt tttttagata aaccaattct tttcttttca attgctgttc
    69781 atattgttct cgttgcctaa tagttattct aataaataaa aatagaacaa caaacatttg
    69841 aatgattaaa aatattaaga caccaaacac aaaatgatca aatacttgat attgatgagc
    69901 aacataagaa acaaataaac caattgtaag taaataaatc atgaatatag cagtaagttt
    69961 agaactggac tgttcaacaa tcctatttat agctaatttt ctatataacc aaataaacat
    70021 cagcaaaaac aaagaatcaa gcattatctg gaatactaca taaccatacc cttttatccc
    70081 ttcaaatgaa aacatatacg tcataaccat ggaagtaata atagatctga aatcatcaat
    70141 aagcatacat aatagtaatt tatttaaaag cactatatta gttttcttct tttcctttaa
    70201 tataaaataa cttccgatta taggtatagc tgcggaaaca tcagtaaata attcaacaaa
    70261 aaaagtgagt ggtatcaata aacatacaaa aagatatttt cttttgttaa gatcagtatt
    70321 agtcatataa aataagagaa aaaagaaaaa gtttcccacg aacattctta atagataagc
    70381 ttttgtatcg atagtaatca agagatcccc tcctgatttt aggatatcat aaaaaaaatt
    70441 ttagttacac tttttaaata atgaaaaaac acattccact aaatttgatg ccggttttcc
    70501 ttgaggtttt gttccagcac ctcctttaat ttttttaagc tcttgatctg aacattgttt
    70561 agcatttagt ctattttttt cttccattac gattccccct acaaactata aatactttta
    70621 tgtcactgat tcaatatacc tatttttcta atcaaaaaat tttgagacat atttaatatt
    70681 ataagtatca ttaaaattga gatttatctc cataatctgc tcgatataaa ccatttattc
    70741 cagcagttct tccaatttca ctcaaatttc ttattaattc ctcttgatat tcagtgaatc
    70801 ttattttatt agtataagca gtaaaatata gataattcac caatctattt attaaaggac
    70861 cgggatctaa ttcatatttt tgtttttcta attttaaata taccttttgt aaaacctcaa
    70921 gaatatcaga atatttagga tctttactta tagatatatc attatataat tcatgaacaa
    70981 tttgcttagc attttttttc attttagcac ttccctggaa ttgctccacc taaaaaacca
    71041 cctatagaca ttcctgcaat acaagtagtt gccttggccc aatcgaccgt acatttattt
    71101 ttagtgcaat acactccatt tccataatat tttccactat gagtggtacc cccatagata
    71161 agttgtgtct ctttaataga caaaattttt aaatgtttca taatctaatc acccctaaat
    71221 taatgttttt agactattat tctaacattc attaaatttc aaaaaacaat tccttcttga
    71281 aacgtagcaa aataattcct gaataagaat ttaatcactt ttaagtatat aaactatgta
    71341 tattctttaa tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc
    71401 tttcatacct acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc
    71461 gtctttggac gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag
    71521 agacgaacat ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt
    71581 tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag
    71641 tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata
    71701 tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc
    71761 aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct
    71821 tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca
    71881 attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat
    71941 gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta
    72001 actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca
    72061 tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa
    72121 ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta
    72181 ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt
    72241 gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact
    72301 acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt
    72361 tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca
    72421 ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta
    72481 ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat
    72541 gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg
    72601 cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt
    72661 gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat
    72721 agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact
    72781 tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca
    72841 aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat
    72901 cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt
    72961 tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga
    73021 acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat aaaccataga
    73081 ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc
    73141 gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac
    73201 atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac
    73261 cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc
    73321 atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac
    73381 accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt
    73441 gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc
    73501 gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt
    73561 caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt
    73621 caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt
    73681 ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt
    73741 tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat
    73801 caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat
    73861 cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag
    73921 cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc
    73981 tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt
    74041 aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt
    74101 aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa
    74161 cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc
    74221 tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat
    74281 cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac
    74341 gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt
    74401 tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt
    74461 ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct
    74521 tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt
    74581 agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa
    74641 tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc
    74701 tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc
    74761 aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag
    74821 ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc
    74881 gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa
    74941 aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa
    75001 atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg
    75061 gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac
    75121 aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc
    75181 cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat
    75241 cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg
    75301 ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt
    75361 tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct
    75421 tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc
    75481 atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat
    75541 taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat
    75601 aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta
    75661 aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat
    75721 aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc
    75781 atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa
    75841 gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt
    75901 gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc
    75961 caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc
    76021 cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt
    76081 ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac
    76141 tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt
    76201 gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag
    76261 ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag
    76321 tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg
    76381 ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg
    76441 tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac
    76501 atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa
    76561 gtcgatccat gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc
    76621 ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga
    76681 tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc
    76741 caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg
    76801 ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca
    76861 aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag
    76921 aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga
    76981 ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga
    77041 tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa
    77101 aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt
    77161 catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct
    77221 tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc
    77281 tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc
    77341 cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca
    77401 ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga
    77461 tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag
    77521 gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg
    77581 atccttcttt tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag
    77641 tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca
    77701 ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc
    77761 ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat
    77821 ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg
    77881 gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc
    77941 agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac
    78001 tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt
    78061 acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat
    78121 tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc
    78181 tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt
    78241 gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca
    78301 attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa
    78361 tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat
    78421 acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca
    78481 caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga
    78541 taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa
    78601 ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga
    78661 agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag
    78721 tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg
    78781 taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct
    78841 tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat
    78901 gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt
    78961 cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc
    79021 aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat
    79081 gtcaaaccaa atacgatgac ttttctatct attatggaaa aaatcaatta cttgatcgat
    79141 caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta
    79201 tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt
    79261 tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat
    79321 cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt
    79381 gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga
    79441 cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac
    79501 tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg
    79561 attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca
    79621 agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta
    79681 ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct
    79741 ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt
    79801 ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta
    79861 tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca
    79921 gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt
    79981 gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat
    80041 attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt
    80101 tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta
    80161 ttaggaacgg atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc
    80221 cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca
    80281 tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga
    80341 ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag
    80401 gattcattag actttacaaa tatttacttc atgctgttga attattggac acttgttgaa
    80461 agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat
    80521 gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa
    80581 gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa
    80641 caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc
    80701 tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa
    80761 gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag
    80821 acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat
    80881 gcaatggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa
    80941 atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat
    81001 attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt
    81061 actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat
    81121 agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta
    81181 tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct
    81241 gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag atgattggcg
    81301 tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct
    81361 agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca
    81421 tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc
    81481 ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa
    81541 tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac
    81601 accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt
    81661 ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa
    81721 actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg
    81781 attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct
    81841 ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg
    81901 gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg
    81961 gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat
    82021 ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg
    82081 tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt
    82141 aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac
    82201 tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt
    82261 tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc
    82321 ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct
    82381 gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac
    82441 aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac
    82501 aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa
    82561 gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa
    82621 ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag
    82681 atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac
    82741 tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc
    82801 ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata
    82861 aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta
    82921 ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc
    82981 cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg
    83041 gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact
    83101 aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt
    83161 tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt
    83221 aaaaaaactg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata
    83281 aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt
    83341 ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta
    83401 acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat
    83461 tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata
    83521 ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca
    83581 gccaaacgaa aaatataagg aactattttt atttggctgg gcttattact tgatgtagga
    83641 catctatttt acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga
    83701 tatttttgtg tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat
    83761 caatcgtata gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca
    83821 catcttcgat tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt
    83881 ttctatggat aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca
    83941 ctactgtgag tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca
    84001 tgccgatggc gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg
    84061 tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca
    84121 cttgagcaat cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta
    84181 aagcatctct taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc
    84241 ccatacaaac taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa
    84301 aaccgattgc tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg
    84361 tcaataccat atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc
    84421 agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc
    84481 atagtccttt ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa
    84541 cggcaaaata tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg
    84601 atttcacaac ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg
    84661 atgatcacaa caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca
    84721 ggtccttcaa tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg
    84781 atttctttac ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct
    84841 tctgctttat cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact
    84901 gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg
    84961 attcctaaga tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat
    85021 gctgcttcca gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta
    85081 tcaaataatt ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca
    85141 acgtactcat ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt
    85201 tctttatata aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg
    85261 ttcatacctt ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca
    85321 cgaatgattt ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct
    85381 ttttttcttt ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa
    85441 taaataggaa tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg
    85501 aaaaccaagc aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg
    85561 agttattatg agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct
    85621 ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa
    85681 cggagccggt aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg
    85741 acagatccat tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga
    85801 tgaaacatta acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga
    85861 agaaaaaatt gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga
    85921 aaaagctggg aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt
    85981 agttgctgag atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa
    86041 aaatctaagt ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc
    86101 agatgttctg atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct
    86161 gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt
    86221 tcttgatcag atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac
    86281 tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca
    86341 atatcatgct caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc
    86401 cggttcccga tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct
    86461 gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac
    86521 gacattagca gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc
    86581 catcaatttg accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg
    86641 aaaatctact ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca
    86701 cttcccagat aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt
    86761 agaaacacca ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg
    86821 tcgccaacta gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt
    86881 aagtggtggg gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt
    86941 actgattctc gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga
    87001 cgggatacag aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca
    87061 agatcttgtg caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga
    87121 aaaaaaggag ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca
    87181 attagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt
    87241 ggctattctt attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta
    87301 ttttcgagat ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga
    87361 aaaacaagcc cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct
    87421 agcaatgaac cagccgcacc tccaataaag tacataaaca taaatacagt attatttcgg
    87481 ttgcttgctg cttcacctag cccctgtact cgtgtttgat tggcgacttg accaaattga
    87541 ttcccgacgt ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc
    87601 aataaagcaa taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca
    87661 gaataagaat ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa
    87721 atcgctaata tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa
    87781 atcaacgtag accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct
    87841 tctcttaata gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat
    87901 gaaacagctg tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta
    87961 agaattccga ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca
    88021 acaataccag ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta
    88081 cccatgacct tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt
    88141 tgcggcacga tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa
    88201 ggcgcaaaaa aagcggccaa cagagagacg gcagataacg ctgccattcg aatgatcaat
    88261 tttggacgat ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct
    88321 aattgggtca acatcgtcaa tatcccgact gcacttgtgc taacagataa actagctgct
    88381 acttttgttc caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt
    88441 gataatagta aaataagcga agttgttaaa ggtttttttt cattactctt cattgatttc
    88501 ccccatttcc tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact
    88561 attcgccaca cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa
    88621 atgaagacaa ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg
    88681 ttatatccgt gaaacagagc ttctccacca cacgcataca acacagacga agggatgact
    88741 ccaatcacga tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa
    88801 ttgaccatag aagatggaat aaccggtatc atatagccta acattacacc tatttttgga
    88861 tgtttcattt gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat
    88921 tttaagcgat gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg
    88981 atgatcgcac ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta
    89041 ggaatacctg gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa
    89101 gtcccatgcg aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat
    89161 aattggatgt ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg
    89221 attcccaacg agatcaatat caatcggatt attttctttt tcatatttcc tcctgaaaaa
    89281 aatctatatt cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat
    89341 aggatctcag tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct
    89401 tcaagtttag ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca
    89461 ggagcgccat caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact
    89521 gctttgataa ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt
    89581 tgttcttcag cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat
    89641 acgccaaatt cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct
    89701 tttaactcag caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg
    89761 ttctttttta tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc
    89821 cactgctttg acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat
    89881 agatagaagt ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga
    89941 gactttacct tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc
    90001 gtccatgatt ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc
    90061 agtgaatact tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga
    90121 atttttgata actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac
    90181 ttcatcaact gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt
    90241 ttcgcttact tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga
    90301 gtttcacctc ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta
    90361 tagttacttt gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta
    90421 ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc
    90481 ttcacttcgt tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga
    90541 ctattgaata tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg
    90601 tcttcggtac aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca
    90661 agaattattt ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt
    90721 caacatggat accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt
    90781 tagctgctga tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta
    90841 atttttcatc gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca
    90901 cacggtaagt tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa
    90961 ctgtaccagt tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac
    91021 ctacagtagc catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt
    91081 ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt
    91141 ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta
    91201 atacaacagc accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg
    91261 caacttcaac tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt
    91321 cggcaacagg ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc
    91381 tctttttagc cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg
    91441 ttctatatct ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat
    91501 tctacagtga tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg
    91561 ttagctgcgt ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa
    91621 actttcgcaa ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt
    91681 ttcaatagaa cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat
    91741 acagaaataa caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct
    91801 tttgtgaatc ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct
    91861 ggagttgctt tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg
    91921 agacatacct ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg
    91981 ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag
    92041 tatagcaaat ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa
    92101 taaacgctaa atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt
    92161 tacacttgcg ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc
    92221 cttgtacaaa gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata
    92281 gaagtgtctt tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg
    92341 gagctgctag aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag
    92401 atatttatct atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat
    92461 tagttggcgg cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat
    92521 tggcatatga agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc
    92581 caaactctgt aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt
    92641 cgatcggcgg tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca
    92701 tgggtactcc taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga
    92761 ctagtatact ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa
    92821 aaggtgaaaa tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa
    92881 aaagactcaa acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa
    92941 aaatgttttt atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag
    93001 aattgatgat tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga
    93061 tcatggaaaa aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta
    93121 cctccgtcac aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg
    93181 cagacttttt aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg
    93241 aagtcaatgc aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg
    93301 ttcctggtgt gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg
    93361 aattcttatt taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg
    93421 gtgcagaagg gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt
    93481 tggtttgtgc aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga
    93541 aaaatatgat gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca
    93601 aacgtaatgc tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta
    93661 tcgaaagtgt cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa
    93721 tgccgtcgat tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac
    93781 gacttgcgga agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta
    93841 agagattagt tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt
    93901 ttttattaat ccatcaattt cgtatatttt acttcatcga tcgtttttgt tcctgcaagt
    93961 tgcatcgtga tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc
    94021 ccacctagat tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt
    94081 gccagagctt tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca
    94141 cgtttgttga ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct
    94201 agttgacgtc caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt
    94261 actgcatctt ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg
    94321 attgttttga tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt
    94381 ccttttcctt cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt
    94441 ggtaatggaa attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa
    94501 tctgccgtta aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca
    94561 ttgaatctgt cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca
    94621 tcggctgcgt ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca
    94681 gcagctactc ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct
    94741 gatggcgctt ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg
    94801 gctgagtcga ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta
    94861 ttttctttca tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct
    94921 tccatgcgct cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt
    94981 tcttcattac ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat
    95041 aaatcatact aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta
    95101 ttctttccaa ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact
    95161 atgagaggca ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa
    95221 caacgcatag accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc
    95281 atctagttga ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg
    95341 ctggtaatct tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag
    95401 agactgcgca ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg
    95461 aacttgtggc aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc
    95521 tcccatggaa tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa
    95581 aagacaagtt ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa
    95641 tacttggacc atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc
    95701 atctgttcct actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg
    95761 caacatccct ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat
    95821 agtggttttt tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt
    95881 agtatttatc ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc
    95941 tgctgcagtt gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc
    96001 catatagaaa aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt
    96061 attaatcgat tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca
    96121 tatcgatgtt gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta
    96181 atccagcaaa tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat
    96241 tttgtcgcag gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg
    96301 gtgctggttt gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc
    96361 ggacaacata ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg
    96421 ttttatgaac gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa
    96481 caacgacacg gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg
    96541 cttttacttt gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag
    96601 attccattga aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt
    96661 tttctttcgc ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc
    96721 cagccaaata agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata
    96781 gaagtttcga tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact
    96841 tgtttgattt catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg
    96901 tttatttcgt ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt
    96961 ccaaacgttc gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa
    97021 cggaacaagc gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc
    97081 agacttatac ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact
    97141 atctattgtt ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca
    97201 attggaggta tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc
    97261 gttacagaga ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt
    97321 tgtgtgccga tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat
    97381 atctatcgtc ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa
    97441 aacgctacaa agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa
    97501 gagcttcctt atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat
    97561 agtacgttgc ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg
    97621 acaattgctt ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa
    97681 cacagcagca ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt
    97741 acattgatca cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac
    97801 ttagagaaaa agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa
    97861 tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga
    97921 cacgcttttc aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca
    97981 tgacgataaa ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg
    98041 ttggaactaa catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata
    98101 ctaaacgcat ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa
    98161 gcaatgtttg gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat
    98221 agacgatgac cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat
    98281 tcaaactttc tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag
    98341 ttaacgctag ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc
    98401 tgcttttcgt tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg
    98461 ttgatgaacc gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca
    98521 atccgtctta tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa
    98581 tcgtgggata tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg
    98641 aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg
    98701 aatttgagca acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca
    98761 tgccgaacct tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt
    98821 tgtgtttttc ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga
    98881 attaattttg taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa
    98941 agcttgctaa ttattggtta accagcaaac aggagtatta aaatatcttg ttttattgaa
    99001 tagcaggagg aaatcatgaa attccattat catatattga tcaatccatc ggcaggcagc
    99061 ggaaatggct ataaagtagc agaaagaatt ttaccggtat taaaaaataa gcatattgac
    99121 tatacacttc attatagtga atacaaagga cacgatgctg agattgctga aactttggca
    99181 aaagaaacat tgattccttg gatagaagaa gaacagaaaa caatcgatac tttccccctt
    99241 ttgatcatcg taggaggtga cggcacctta catcaggtac ttgatacttt ctatcagatg
    99301 gaagtagaat tcccagttgc ttatattcca gcaggttctg gagatgattt tgctcgaggg
    99361 gcaggccttc caaaaaatcc caaaaaagga cttcagctga ttttagcagc tcaatcacca
    99421 gaaaaagtcc acataatggc ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt
    99481 aataatttcg gtataggatt agatgcagct atcgtccatg cgacaaatca ttcttccaca
    99541 aaaaagcgat tgaacaaata caacttagga tcattttcgt atttattctc tattctccgt
    99601 gctttattca ctcaaaaagg atttccaatt ctggtagatg caggaggaaa acaattaagt
    99661 ttttcaaacg catttttatg tacagcaacg aagcatcctt tttttggcgg aggaatcgcg
    99721 attgttccaa ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc
    99781 aatttcttta aaatcttgtg gctcattctt ttacttatac agaaaaaaca attgaaatcg
    99841 aaatatttcc atcactacac aacaaacaga ttacgtatcg tttcaacaac accgcaacat
    99901 ggtcaagaag atggagaaga aatggaaaaa cgaccttttg atatcacaat aaccacaact
    99961 aaacagttgt tttggtgtta ataaaagagg ttttgtcaga agctacatct ggaacaccgt
   100021 ttattcggaa aaaaggactg tgacacgact tttgtcacag tccttttttt actatgcagt
   100081 taattctttg cccaattggg ttgccatcga tagtttcttt ctttacccaa aataaattgg
   100141 atcattgaaa acaccgcagc aaaagcaact aagacacaac cattgaacca aaaagattct
   100201 gcaaatgttt gattgtaact gagtgtttca ctgatcaagt tattattttg aacaaaccaa
   100261 tatgttggtg tgaatgccgc tattttgcta accatttctg gcaagtattc tgctggtaca
   100321 aacaccccac cgataaagca actgcctgtg ataaaaatat ctttgatccc accgctggtt
   100381 tctgagtttt ttaccaatga agtgatacaa gccgaaaagc taatgatcgg caataaaaac
   100441 aagaaagtat ttaaaatatg ataccatacc gttatatccc acgaggtgtg gatcactaac
   100501 agaaccatta gaagtaagaa gccactgaac aacagcagcg agtaactaat caaactaaag
   100561 gttactttgc gattcaagtt tcgtttggta atgggcgcac tctggtttcg tttactgatc
   100621 tctttgcgat taaatgctag attgatgacg ctgaaaccgc taaaaatgga tagaaacaac
   100681 ccataagaca agaaattaaa ggttgtgccc gtcaacagct ttttcatccg ctgactatat
   100741 cccgcactta gctcaacttc tccagtaacc gagagtgcca tttgtgtctg ttccatagct
   100801 gcatcgattg ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact
   100861 tgctgtgtaa caactgtttt agaaaaggca tctgggcgac tctgtatctt gatcgtcggc
   100921 atctttccta cttcgacttg ttcggtaaag tctgcaggca gatgtaagat aatgtttaca
   100981 cgattaaaat aaagagcatc atcgatttct ttttgtgagg ttccatccaa tgtaacgact
   101041 gtttccttgt cattcaaata tttacgaagg ccatcgacta caggcgactc ttcttcacta
   101101 atcaaggcga ttttcgcatc ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa
   101161 aagaatgtgg caacaacagt gataattaca cctatcagta aattcgattt atttgcttta
   101221 agcaatgtcc aaatcgtttt ataaagcgtc ataacgcacc cctctttcca tccaagcatt
   101281 tcctaacgca aaaaggaaaa taaatccgat caaccataga agattggtat aaaaactagc
   101341 caaatcagtg tagaaataaa gtttatacaa actttcgctg atcaagttga cgagatttaa
   101401 ttgaccaaca atcggaatat gaagatcaat ccaatacttc acttgttccg accccatcat
   101461 accagcaaaa aagctcatcg caatcgtcac tgaaatcaag atgctatcct tttgcaccag
   101521 attcattttt ggtatcaaat taccaaccaa tgtacctaac aaaatcgcac acaacgaccc
   101581 cagagcggta acaatcatca accattgcca acgttcacca aagtctactt gatagataaa
   101641 acgaaagaca gctaacataa tataaatctc tgtcaaaaca atcgtaaagc ttgctgccaa
   101701 attagcgatt gaaactaacg attgctttct aggaatcaag gacaagcgaa taccatttga
   101761 cgattgattt gcttgctgat cctgtacatt gcggaccccc cacatcgtcc cgtagagaat
   101821 cgccattcca atcagagtga agaaataaaa acttttgaaa ctcatattac gacctgcatg
   101881 aacttcttga aaagatactt cttgttcaaa aacagtcatg atgggcgcga gatcaacgtt
   101941 ctccacttct gccactttac tcgaaattat accaatacgc tgtaaaaatt gatccatgac
   102001 gtttttcatc aacgtctgac tcattcctgc ttgacccaca tggagcgaaa cagatgaacc
   102061 atcaatgaga taaaaaccag ccaaatcatc tgcatttagc ttttcattcg cttctacttc
   102121 cgatagttcc tgtacttcaa ataatggttt atcattgact tcgacttgtt ctaagactgt
   102181 ggtaaaattt tccgaaagcg ctgtttgatc cgctttgatt acaattccag tattaatcgt
   102241 ttcgagattg ccaccttgat caatctcgcc aaaggccgcc ataaacatca atcctaaaag
   102301 aattggaaag gccaatgtcc aaaacaacat ggatttattt cgcagcagta tttttgtgcg
   102361 atatttataa aaatgccaaa acattatttc ccctcctcta gtctctcaaa tccttgccag
   102421 taatctctaa aaagacatca ttcaaggacg cttctttggt tgctattttt ccgtaactga
   102481 tcttttcttc ttgtaagatt gtaagaatcg gcaacaatac tgtatttagt tcagtagttg
   102541 ttacttggag ctgacctttt ttataagcga ttccatcaga agctattgtt cgctgacaac
   102601 acgttttctg ggagtaatgg gatgtcgatc gtcaaacgat ccgccgtccg taccatatct
   102661 tttagagatt ctttggttcc tttagcaatc acctgtcctt gatccatgat cacgatctgg
   102721 tcgcaaagaa tttctacttc ttccatgtaa tgtgttgtat agacaatggt tgctccttgt
   102781 cgattcagct ccttgatact ttctaaaatt ttattccgac tttgggggtc tacagctact
   102841 gtcggctcat ctaaaaaaat taaccgcggc ttgtgggcaa tcccacatgc gatgttcaac
   102901 cgacgtttca agccaccact aagttctcct ggacgaaacg aaacatattt ttctaatccc
   102961 actaattgaa tcacctcttc aatcaaggct ttccttttct ctttttcgga aacatataaa
   103021 ccgcaaaaat aatcgatatt ttcttgcaca cttagttcgt caaagaccgc aacgtcttga
   103081 ggaacgaccc caatcattgc cttgacagca taattttcag gagtcattgt tgtcccgaac
   103141 aatttaatgg ttcctttgtc ataatttaac aaagaaagca tacagttgat ggcagtcgat
   103201 ttcccactgc cgtttggtcc taacagacct aagacttctc cttcttgtac ttgcagatca
   103261 aaatgattca atgctacaaa atctccatag cgtttgacta atttatcaat ttcgataatc
   103321 atcatgaaaa actccttcca aatttcttgt tgatctgact ataataaagt gcaagaaaag
   103381 aaactagtga cgattggcta aaccacacat gacatttgtc acaatgaagg agtctgctat
   103441 gcataaatct cggctactca acgaaactag ctttctactg gttatctcat tgcttttgct
   103501 ggtaagtcaa ggattcgttg ctatgcaagt tatctattta ttgatcgccg ttttattcac
   103561 tggtgtcagt tatttactgc ctacaaagcc aaattggatt ttccaagtta ttctatttct
   103621 tttatgtgcc tttttacctg atcttgtcct gtttctgcca gcgacgatgc gagtgatcgc
   103681 ccctgaagaa agccaccttg attacttgtt tttgttaggt tttttgttga tttcgacggt
   103741 tggtaatcat ccccccatca aggtcgcctt tgtcgtaatt ttgctgtttt tttcctttta
   103801 cttacgaaaa aaagatcaac gaacgaccga tcttgagaaa aagtacattg tctccaaaga
   103861 tactcattgg gaaaaacaac aagagttatc ggaacaaaat aaagcattgg ccctgtcaca
   103921 agaaacacaa ttagccttgc aaattgtgga agaacggaac cgaattgccc gagacatcca
   103981 tgacaatgtc ggtcatctgc tgtcaagtgc gattttacaa attggtgcgc ttgaaatgat
   104041 caatcaagaa ccaaaactaa aagaaccatt agaaaattta agtgccaccg ttcacactgg
   104101 gatggatcgg attcgtcaaa gtgtccatga cctccatgag acatcgatct cttttcaaca
   104161 ctccttagag tttctgatcg ctgattttcc ttcttctgtt acgattgaag gaaacttgtt
   104221 tgaaggattg aatgaaacgc agcaaaactg cttcttaatg gtcatcaaag aagccttaac
   104281 caacagtatc aaacatagtc atgccaaaac cgttgtttta cattttcgaa cgttgccggc
   104341 cttttatcgc ttgcaaatca cgaatgatgg caccagccca caaaaaaatt ctgctcccgg
   104401 tattggtttg aaaacgatgc gtcaacgtgt tcaagaacaa aagggacaag tccatatttt
   104461 ccaagaaaac cagcaatttc agttaacgat cattttacca agggagggac accaatgata
   104521 acagtagtca ttattgagat gatccatttg tgacgaaatc gttacaaacc attttagaaa
   104581 gcacgaaaga gatccgtgtg ttaggcattg gtcattgtgc caaggatgcg ctagtcttat
   104641 acgaaaccca tcgtcccgat gttttgctga cggacatccg catgcctaaa caaacgggca
   104701 tcgatgctgc caaagaaatt ttggctaaat ttcccaaagc catccttttg cttttgacga
   104761 cattcaaaga tgaagaatac atccgcgaag cattttccat cggtgtcaaa gggtatttaa
   104821 tcaaacagaa cttgcaagcg attattcctt ctgtcaaatc cgcttataat ggacaggccg
   104881 tatttggtaa tgaaatcatc gagactttca ctcaaatgat gaagaataaa ccgacgattg
   104941 atagtcatgc ttatgcttct tttagtgaac gtgagcttgc aatcatcaaa gaagtagctg
   105001 ccgggaaaaa caataaagaa attgcagatg ccttgtactt gagtgacggc actgttcgca
   105061 attacatcag ccaattacta gaaaagctag atttacggga tcgcacgcaa ttggctatct
   105121 atttttatca gcatttgcaa taagtcttat ctaacacaaa aaaacctgaa tgatgctcct
   105181 tcgcatcatt caggtttttt tcacatcttc tattcaattg tcccgcaaac catttcctca
   105241 cgattgaaag aatcaaggat tcgcagggct gccaggattc gttggattgt tcggcgtgtt
   105301 aggcggaaaa ataggcgcct tgtctaacgt tttttctagt tttgattctt cacgatcgtc
   105361 tcgttctaca tcaggacttt cattcaattt gaattctttt tccatctcat ctaacttttt
   105421 aaagtcattg ccagattctt ttgctaattt cgctaattta tcttcggttt ctttagccat
   105481 aaagtaacgc cccccttttg atttcagctt agcatatcac actgaaaaaa caaaagaaaa
   105541 cgctaaccaa cgatcatttt tagctatttc tcatctttaa aatcaagcat tttttgccgg
   105601 tataaaatcg tagggagaac tgttttccat accaatcatc ggatctacta actggttatc
   105661 gatcacttct gctgtaagaa cgaaatcttt agaaacagca ggttctacct cttctttctt
   105721 ttcttctcca acaaatgaag aaaacagatc tggttccact gtttccatta cttggttttt
   105781 cgtttcattc gtcccactgt catcttctac aggtagcttt tcttttacaa cccgtggctc
   105841 ttttgtatag ttcccatctt caaatggatc atttccgacc aaagaatctt cataagctgc
   105901 tagtttggtc cggagagtga tattcatttt atcattatcc atgatccttg atttcaacgt
   105961 agtcatccgg cttgctgatt cattcgctac acaagtgaca taggcttgct cttcccctaa
   106021 caaaatcaac agttccttac tgcgagtcac tgccgtgtag agcaaattac gctgaagcat
   106081 ccgctgatat tgatggacca tgggcaaaat aaccatcttg aattcactgc cttgtgattt
   106141 atggattgag caacaataag aaagagtgat tttattccat tcgtttcttt tataagtgac
   106201 ctcatttgca tcaaactgga taactaattc atccacttta tcttcagaat cttttgccaa
   106261 gatgataccc gtgatttctc ccatatctcc attgaataca ttcaattcgg gagtattgac
   106321 taattggagg actttgtctc ctatccgata aacggtgtcg ttccaatgta cttccttttt
   106381 tccaggtttc cctggattga atatctcctg catcatttta tttaacgcat cgatccctgc
   106441 aggtccccga tacattggtg ctaatacttg gatatcttgt gcacggaatc ccttattttt
   106501 cgctttttcc acaattttag agatcaaggg ttcaatttga taagcatcac ttcggaaata
   106561 ggaacgatct ttttgattct ttgtgaaatc ttttggcaga cgtccttctt tgatctcatg
   106621 ggcaagagga ataatactag aaccatcacc ttgccgataa atctgattga gttccatttt
   106681 gggaatttgt tcgatttcca aaagatcatg gagtacttga ccaggaccga cagagggaag
   106741 ctgatctttg tctcctacta agataacttg catattcgtc ggaatcgctt tgaataatgt
   106801 gttagccagc catgtatcga ccatagacat ctcatcaaca atcaaaagtc cgccttctaa
   106861 ttctttcgta ctgactgaag gtgttttttc ccgtccattc agccccagaa gacgatggat
   106921 cgtactggca ggcagacctg tcgtttcgtt cattcgttta gctgctcggc cagttggtgc
   106981 tgcaagtaga ataggaaaaa tttcttgggt atattttgaa ggatccaatt ccagtccgtt
   107041 caactctgca aaaagattga cgattccgtt gatcacagta gttttccccg tacctggtcc
   107101 tcctgttaaa ataaatagag gggaacggat tgcttcagcg atagcttgtt cttgcgaagg
   107161 tccatactgt atgccgaata ttttttcgat acgccgaata ttcttctgca cttctttttc
   107221 aggataatgg atttcttttt tacgactgag aaggcgctgg atcgaattac cgatccccca
   107281 ttcagaaaag tacaaactgt tttcgtaaag attagtctct tcttgttgta tctttccttc
   107341 ttcaacaagt tggataatta cagtagcaac ctgatcaggc tggatttcaa ttgggcgact
   107401 tgtttctaaa atacggatcg tttgctgaag caatgtctcc gccggtacgt atgtatctcc
   107461 tgtttgaact gcatgctgaa aaatctgatg caagatagct gctcgaattc ttctcggtga
   107521 atctgctgca ataccgattt gttcggctag attatctgca cgtttgaacc cgattccttc
   107581 tatgtcttcg actagctggt acggattttc ttcgatgata tccaaagact cgttcttata
   107641 ggcctggtag atactgaaag ctaactgact accgaacccg taacgattca gtcccatgat
   107701 cacttgatcc atcccgtggt ttagccgaat cgtctcagca agcatttctc tttttggctt
   107761 cgtcaaaccg gagatttgtt ttaataccga aggatcagca atgatttttt cgatagcatc
   107821 ttctcccagc aattctacaa ttttttctgc tgttttcttc ccgattcctg ggaatttttc
   107881 gctggaaaga tacgctacga tcccatttgc agttgttggc ttttcttgct catatctttc
   107941 gagtttaagc tgccttccgt atttaggatg atccactaat tctccataaa aacgataagt
   108001 ctcattttct tgaatatcgc cgaaacttcc ggtgactaca atctcttttt cacggtaatc
   108061 agctgtggtt tcactgatgt gtaccaaaag cactttgtaa aaattactag gattttgaaa
   108121 aaaaatcgcg gcaactgtac caacgaaata aggcatttct tctggctgca tcggaagccg
   108181 cctccttctt ctatagtaaa ggttctggtg ctgtttcagt tcctaaaaaa tctgtttggt
   108241 tccatacttt cagttcatat ggcaataatc tgcaaggctc ttcagtctct aaaatcgtca
   108301 aactactatt gaacaatcca cccatttcac gcaactcact tagctctttt cctgtcatcc
   108361 attgaatggc tgcagttaaa gaagctccat gtcctacgaa taaaagtggg ccatctttat
   108421 gttgggcaac cgcatctgct acaacagttt cgatacgtgt gattgccgat tcaatcgttt
   108481 ctccgtgaaa aggagaagga tcatacagat ctaaatgatt acgcaaattc ggcagattat
   108541 ccggatattt ctggtacatt tcatcgatcg actgaccttc taaagtaccc agccctagtt
   108601 ctcttagctt atctgtataa aaaatctcta ccggacgagc aagctctttg ttgatacctt
   108661 ctgctgtatc acgtgctcgc tttgctgtac tggaataaat tgctttaaaa ggaacatatt
   108721 ttaccttttt cccaaatgct ttgatttctt cgtaactttg tggcaaaagc ggagaatcgc
   108781 cattgctccc ttgaaaacgt ctttcctgat tccattgtgt cttcccatgt cgggtaaaat
   108841 atagttccat acgcagcaaa ctcctgtctt tcttttattt tactctataa taaactgtct
   108901 aatccagctt gtcattttca tagtgatgcg tcaattctaa attagggaat ccttgctggc
   108961 gaagtgcttc atacactatc attgcagctg tattcgataa attcaatgag cggacatgct
   109021 catcattcat agggatgcgt aaacactttt cttcgttttc acgcataaat gtttctggca
   109081 aaccggttgt ttcttttcca aataaaaaga agtgctcttc tccatcagca aaattttcgt
   109141 cactatatac tttatgggca aattttgaga tcaaatgtag tttacgatta cctaaaaatg
   109201 ttaaaaaagc ttctaaatta ctgtggtaag taatatttac atcattccaa taatccaacc
   109261 ctgcccgttt tagatgtttg tcatcagttg aaaaacctag tggctcgatt aaatgaagcg
   109321 gagtattcgt ggctgcacac gtacgggcaa tatttccagt gtttgcaggg attaatggtt
   109381 caaataaaac gatatgattc atcaggatca ttcctctcat atttatgttc gtttctattt
   109441 tctgacaatt tgatttttgc ataaaaaaaa acgtatcaac tcggtgtcag tcactgcgag
   109501 tcgttacgtt agtgaagctc ttctcactaa cttttatcag aacaggaacc tgataaaaac
   109561 caatgaaaaa acaacttgtt gctaatataa caccctcaac ttttttttgc aacctctact
   109621 cattaaaaac tgctagaaaa acgttttctc ttagttcctt ttaataattc tatgtcaatt
   109681 gtaatcttct tcaaaggatt cttatataag tctgctttga ttcgctcttc tacctgccat
   109741 tctaaaggtg acatggccaa aagatctttt tgattttcct ctggtatctc aaattcatag
   109801 gttacacgct gacgttcagt taatttcatt gcttcagcaa acttggctac tacacgttca
   109861 ttggaataac tctgtttttc aggttgattc ggataaaaag cctgacgtag ttcctttaaa
   109921 taattttctt gtggcaccac cttcaacact tctccatctt ctgagagtac acgttggaat
   109981 tcttggtaat gagaaggcga aaagatattt aaaaccgcgt tgaatgtttg atcagcaaac
   110041 ggcaaattgg tcaaatcagc tacacaccaa aatgcttcgg cagattgttc tgtagctgcg
   110101 tagactcctt cttttgaaat atcaaatcca acagcatttt gaatcttttt caattttgaa
   110161 agttcattca agaagctacc ttctccgcag ccaacatcta atagcgagac ttcttcaggt
   110221 aaatgctgcg caatagtctc aataacgggc tgatacatgc cagattggat cattctacgc
   110281 cttggaacga acattccttg atcgtattct gttttaaccg catgggataa aaaataaagt
   110341 gttccttttt tggaaaggtc aaaacggtga ttatttttgc atatcaagcc attatcagac
   110401 ttttttactt cttcttggca aaccggacag cggaacagct gatgattttc tgccaaaaac
   110461 tggcgtcctc ggtcaatttt ttttaacata gttccccctc atttctgttt tatcttaccg
   110521 ttagatgctc actttggcaa ctgctaaaaa tatgatacaa tgcctagaag aaagtgaaag
   110581 ataaaggaga agattagatg atcaaagaat tttgcgctga aaactacaca tctattcctc
   110641 tagcaattgc tagaggagcc gatcggatcg aattgtgtga caacttagcg gtcggtggca
   110701 caaccccgag tactggtgtg atcgaagaaa cgttaagtta tgctggggaa aaagatatcc
   110761 ccgtaatgac gattattcgt cctagagggg gcgacttcgt ctataacgat atcgaattga
   110821 aaatcatgca tactgatttg attgaagcaa agaaattagg gacggatggc gtagtatttg
   110881 gttgtcttac tccttcaggc tggctagacg aagaggcctt agaactattg atcgaaacag
   110941 cagaagggtt gcagatcacc ttccatatgg cttttgacag tattcctgaa gatcggcaat
   111001 tcgaagcaat cgattggctg gtagaacatg gtgttgaccg tatcttgact catggcggtc
   111061 ctgctgacaa aactattgta gaaaatttcc cacggcttaa agaattgatt gcctatacag
   111121 atggccgctt aatcattttg cctggtggtg gtgtcactgc agagaatgca gcacatgtag
   111181 ctaaagaatt gaacgtaagc gaagtacacg gaaccaaaat cgtggatctt cagttataat
   111241 cttataaaca aaaaaacatt tgcaacaggc ataatgctta ttgcaaatgt ttttgttttt
   111301 ttacataaaa tagcccataa ataaagaagc aatattgaaa taaatcagta cactcgtcaa
   111361 atcgctcaaa gtcgtgataa agggaccgct tgctactgct ggatcaaatc ctagacgatc
   111421 cattagcatc gggattaaac tacctgcaag attcgccact gtgattgcaa atagcatagc
   111481 catccctatg acaaacccca agataaaatt atgtttccaa aaacctacga ttagaaaaat
   111541 cgtgatcccc gtaacagctc ctgtgacaac gccggtcaag acttcagcca aaacaagtcg
   111601 aacaaatcca tgatctttct catcagaaat cgcaagtcga cgaacagcca cagctagcga
   111661 ctgggttcca gcattaccgg cagttcctgt aatcagcgaa atgaagactg ctaaaatact
   111721 cgcttcactt accaagtctt catagtgact gattaaagaa gcggtcgaca tacctaaaaa
   111781 caacagagtg atcagccaag gcaaacgtcg agacgctgcc tttactggat tttcattaat
   111841 ttcttccaca ttgaccccgg caagtcctga ataatcgcta gcagcttcat catcaataac
   111901 gtcgatgata tcatcgactg tgacgattcc taacaaatga tcatcataat cggtaacagg
   111961 aagagctaag aagtcataat ctctaaatgt ttgtgccaca tcttcttggt cgtcgcctac
   112021 atgaactgaa agcactcgtt cgctcatcag atcagaaatc attgcgtcgt catcattcac
   112081 gattaggtcg cgcagagaaa taacccctac tagctgacct tcttgattaa cgacatagat
   112141 gtaatagatt gtttcagcta catctgcttc gttcttcagc acgtacatcg cagagcgaac
   112201 agtttgattg gctacgatcg aaacaaattc agttgtcatg atcgcgccag ccgtttcatc
   112261 ctcgtaatgg agaagttcct tgatttcact ggcatcgtct gccgacatca aacttaaata
   112321 tttagcaatt tgctttttat ccagcgtatt aagcaagtca acagcattat ctgtatacat
   112381 ctcagaaagc atatcagctg catagctagg acgcatttca tctaggtatt ctttcatatg
   112441 ttcgttgtct tcttcaatca catcgaacat atccgctaat tctttaggcg acagatagga
   112501 atagatcagc tgcctgttct cttctgtaag agattggtag aattgcccct gctcatagat
   112561 atgcatctct aaaaaactgt ttctgaatgc ctgaatatta ttttccctta aatcttgagt
   112621 caactgggca aatctttcct ctaattcttg attttcatcc aaaccaatct cacctcgcct
   112681 tattcttcaa accgtctcac aactgcctcc atgtctgcgg ctaaaggctg cttgaactcc
   112741 agcaactctt ctgtaaatgg atggtaaaaa cgcagatcat aacaatgaag ggcttgtctt
   112801 tttattccca gatccattcg cccgccatac atatcatcac cgagcagggc acagccaatt
   112861 gcggaaaaat gaactcttat ttgatgtgtc cttcctgtat gcagctggat atcaactaac
   112921 gcgatttctg ttagtcgctt ctctatccag tactcagtct gtgccgaacg tccttcatca
   112981 gagaccattc gcttcaatag cgaagaataa tctcgggcga tcggcaaatc gatatgtcca
   113041 tgtgccgata aatctgaaag ccttccactg actaaggcct gatatttttt cacaaacttc
   113101 ttttcacgaa gttgcatatc taattttgcg tgcgcaaatc catgctttgc aaaaagcatc
   113161 aaaccagatg tatcccgatc tagtcgcgtc accacatgga ttacttggtt ttcgtaacct
   113221 tgttttttat aataagcctt tacacgatta gccattgtcc catttggatg gtattgggca
   113281 ggaatagacg aaacgcctgc tggtttattt actaccagaa cgtgttcatc ttcatacacg
   113341 atatcgatcg gtgtttcatc caaaagtacg gtttcatgtt ctccttcagc aggaatcaca
   113401 atggttacct tatcatcctt agctagagaa aacaagacat tttctacttg atcattaact
   113461 ttgatttgac cgccttgaaa ttttattttc gctaacagac cctttgagat ccctttttct
   113521 tttaaaaaat attttacttg ctgagaatga tctttctcat aatcccatgt aaactgcata
   113581 tcaatcctca ctaataaagg catctttcac acgatgccaa aaatgcatat gtcgataaga
   113641 agcaaaatgg atccgttctt ctgctattcg ataataaatt gagcggatat ttgcttttgc
   113701 gacattcaat tgatcaaccg ttacaagata atcttctgta ttttccaatt ttatttctag
   113761 ccattcatca tgggcaatca cgatcggaga acccaaagta cgaaacactc gattattcag
   113821 tgaagcaatc tctgccaact gaaaggcatt gatactagga tggatcacag caccgccgac
   113881 acttttatta tatgctgtcg aaccagtagg cgtagaaatc gtcaatccat ctcctctaaa
   113941 actttcaaac agctcgtttt tgatgtatac atcggctacc atcgtacgat tggcccgttt
   114001 aatcgttgat tcattcagtg ctaaaaaatg ctggtccggt gtttcatcca aataactgat
   114061 ccgaacatcc agcaagggat aactaacact ttgttcccgg ttcgttcgta agctgtctac
   114121 tagttcttcc agctcgtaat cccgccaatc cgtgtaaaac cctaaatgac ctgtatgcac
   114181 gcccaagaaa cgcacatcat tcagacaatg actgtagtga tggaacgccg acaataatgt
   114241 gccgtctccg ccgactgaga tgaccaattc tggctcatga tcatctcgtt gaatccctgc
   114301 ttgttctaaa agtgcgttca aacgttctgt cacttctata gtctttgctt ctttattatg
   114361 aacgatcgct actttcatcc cctgcctcct ctctttcgct ttctttcttc aaaagcgttc
   114421 gatagtagac atcatctgat tttcctcttc catgggagaa taaccgctgt gcttcttgga
   114481 tttcttcacg gatactggac atctcttcat ccaactgata ggcagcttct gccgcacgct
   114541 gaagtcgttc actcatttct tctggaaaaa cgccttgata tttataattc agcgaatgct
   114601 caatcgttgc ccagaaattc atcgcaagag tccgaatctg gatttccgcc aaaattttct
   114661 tctcaccgct gatcagctgt acaggatact cgatcaccag atgataagaa cggtagccac
   114721 ttgctttttt atttgtgatg taatctcgtt cttggagaac tttcatgtct tttcttttac
   114781 ggatgatctc aacaacttga tggatatctt ctacaaactg acacatgatc cttaacccgg
   114841 caatatcaga catttcttct tctaaacgtc ccattggaat atgccgtaat tgtgattttg
   114901 ttacaatact atcgactggc ttgactctcc cagtgacaaa ttcaatcggg acatgtcgat
   114961 tttgttcacg gaactgtttg cggatccccc gcagtttcac tttcaattct gatacagttt
   115021 gttcatatgg tgctaaaaat gcttcccaat ctcgttccat agctgatacc tctacttcat
   115081 taacattcat tgtttattct accatatatg caaagttttt tcgtactctt gatttatgca
   115141 ttgaccaaaa aaagaaaatt aggcaaaata gacaaaaagg agtcgatcaa atgacacaaa
   115201 tgcttgaaat cgaatataaa tctatgctga caaagcaaga gtacgagata ttcatcgctc
   115261 actatcaatt aacagataaa gatttccgtg tacagactaa tatttatttc gataccgttg
   115321 atgaacagct gaagaaacaa aattgtggcc tacggatccg tttcgttggt catcaagctg
   115381 aatatacatt aaaaacacct gctgaaaaag ggcgtctcga aacaacggat acctttataa
   115441 cagaagaagc agaagcattt atcttcgaaa aacgtctgcc tcgttcaggc gcagtttttc
   115501 aaaaactttc tgatttgaat atcgatcctg cttcattaat ccaaaccgga aagcttacaa
   115561 caaaacgggc tgaattccct attgaagaag gacttttagc tattgatgaa agctggggag
   115621 acaatctaca cgattttgaa ctggaactag aggtaagggg atgcatctgt gggaaaaatt
   115681 gcttttataa attttttaaa acgtttttct ttaccttatc gtcctgctaa gaacaagatc
   115741 caacgtatgc ttgaagctaa aaattagaaa cctttcattt tcacaaggca cttgagttca
   115801 tttttttact tttttctgtt aagttatagg atgtgaaaga atctagtcac tagttgtata
   115861 gaatctattt tgggggagat tcatatgatc gaaatctatt tatttgttaa tcctttaggg
   115921 gaatactgtt tcgaaatgga acaacagtta ttacaattta tcgaagaaga gtacgggaca
   115981 ccatcaaaag aaaagatgca atttcgcttt cttcctttgg tcaatttgca gacaattggg
   116041 gatgtcatgc aacaaaaagg aatttctcag aacaatcttg aggaacgtaa tcacttattc
   116101 tcaatgactt attctgcagc attggactgc aaagcggcgc agtttcaagg aaagaaaaaa
   116161 ggccgccgtt ttttgatcaa attgcaagaa gcagtcggct gtaatgacat accatattct
   116221 caagaacttg ctgaatccat atttgaagaa atcggcggcg atttgaatat gtttaaagaa
   116281 gatcgtcagt ctgacttcgt taaagaagta ttcttttctg atcaaaaaat tgctcgggaa
   116341 atgggcatca caaaacaccc ttctgcagtc gtttacaatt atgcctgcga ccgtgatttt
   116401 ggtgttttag tagaagtaaa aggtacaagt agcctttgga gaatcacaga gctgtgtaaa
   116461 acaacagata actcatatca agtttttaat ggtgaacatt tacatacaaa tgattctcag
   116521 ttccattcaa gaactagcca tttacacttg ctttctaatt aaaaaaatac gtcgactccc
   116581 ttacaagaga gccgacgtat ttttttcgtt tctattcgag aaaatcaaaa atagaaagcc
   116641 ttataaacga tcgatcaatt cttctaattc atttagacgt tgttcaaaca tcgacattgc
   116701 atcttcaata tatgcagcct gtgtcatatc aactcctgct tttttcatta cttccacagg
   116761 ataatcgctg ttacctgctt ttaggtaagc caagtagttc tctagtgcct ctggttcctg
   116821 gtttagtatt ttcttcgcca gtgctgaagc agcagaaaag ccagtcgagt attggaaaac
   116881 atagtaatta taataaaaat gcggtatccg tgaccattcg aatttaattt ctgggtcttc
   116941 ttcgactgct ggaccataat attttgcatt caatttacca taactatcac ttaggtattc
   117001 actagtcaat ggcacacctt tttcatcttc cgtatgcatg aaatgctcga attcagcgaa
   117061 ttgtgtctgg cggaaaactg ttcctttaaa cccatccaag taatggttga gtacataagc
   117121 ccgtacacga gggtcttttt ctgtttccaa taaatactcc gttaggatat tttcattcgt
   117181 tgtcgaagca atttctgcta aaaagatgga gtagtcgccg tacacataag gctggttcga
   117241 acgagtgaaa taactatgaa cactatgtcc catttcgtgg acaagcgtaa atagctgatc
   117301 cagcgtatca tgccaattca ataaaatata tggatttgtg tcatagcttc ccgaagaata
   117361 agcaccgctt cgtttccctt tattttcgac aacatcgatc caacgttcag agaatgcttt
   117421 ttctacgatg gccatgtatt cttcacccat tggtttcagt gcttctaaag ctttttcttt
   117481 tgcttcttcg tacgtaaagg taattggagc ttcccctaag accggtgtat aaagatcata
   117541 catgtgcagt ttttccactt ctaataaacg tttccgtaat tccatgtatc gatgtaacaa
   117601 aggcaaatgc ttgtttacca cgtctaccaa agtatcgtat acactttcag gaatatgatt
   117661 attgctcaaa gacgcttctc tggcagagct gtaattacgg actttcgctt taaaattatg
   117721 tccttttata tgtgtgccta aagtagaagc aaatgtattt ctaaattgtt cgtaaacact
   117781 gtacaatccc ttaaatgctg cttcgcgcac ccttcgatcc gtgctttcta gcaactgacc
   117841 atacacgcca tgagataatt ggactatttc accattttct ccttcaatcg ttggaaaaac
   117901 tagatctgca ttattcaaaa cagcaaatgt atcacttgaa gcatcaaaga tttcacctgc
   117961 tccagcaaga agagattcct gctcagcaga taagacatgg gctcgattat ctactatttg
   118021 ctggatataa tggcgataga cttccaattt tggttcttct ttgaaatatt gccaaatctg
   118081 gtcatctgac aattgcaata tttctggttc aaaccaggaa acggcttcac taactttcga
   118141 aaacagactg cttgctcttg cataaagcgc ttggtaatct gtatttccag tgtcttgatc
   118201 gtttttaagg tgcgcatata cataaatgac ttcagtttgg cgataaaccc tcaatacgaa
   118261 ttcaatcgca tttaaaaatt gagaagcgcc ttgatcaagt gttcctttgt gtttttcaga
   118321 ttgttttaac tcttctgata attccaaata tttctcatca aactcttggt cgcttgaaaa
   118381 gatcttggtc aagtcccaag ttaaattttc aggcaattct tctcgttttg gcaactgctt
   118441 tacttccatg aaatccactc cttttcttcc cttagctcta tctatcaaaa tagatcatag
   118501 tctaacatcc acatgtcaca gcatctaact tttctactca ttcatgtgtc tgcttccatc
   118561 aattttagca taactaaatc ttgttttaca tagaaaaccg attcctagcc tatttttata
   118621 cttactattt tttcaaagaa gacaagtgta tgcattcgtt aaaaaactgc tgatagacca
   118681 tctcttcatc aatcatagga aaaagccatt cccttttatg ttcttttatt tggcaaagcc
   118741 atgagttgaa atcaggaact ttctggtttt ccaaggcttc ttcgtacaac attcgataaa
   118801 gaaaaacctg ttcttgtaaa taaaagaaaa accgactttt ttgatagatc cacgatgata
   118861 gatgcaaaag atgtttatga cgcaagtagc attgttcttg tatggatact gtattctttt
   118921 gcttgtgaat aagttggcgt gtcagccatt tcttcccatc ttcactcact ttttctattt
   118981 ttttcatttg gttcatttta atttcgttac tagtgaatat ttctttaagg tccctactag
   119041 aaaaagtcca gcagctactg caaaaggaaa tatgccctga tacagtctct gtaatatgat
   119101 gatacaaata aagcttttgt ttcttgagat cagctttcca gcaatgcact cctctttttc
   119161 tattatagta gcagcaactt ttttctatca atgagaattg cgattgtcca agaaaagaat
   119221 taccaagtat ccaccatact tggtaactgt tattcaaata attttgggtt ctttcaataa
   119281 atctctggtg agacagtcta gaacattgga tttctattgc tagattcgcc aacaaaatat
   119341 ccggcctttg ccgcaacctc ggcaaatagg cttccaaaaa tactggttct actgactgtc
   119401 ccagccaatc catgaagcat tcctttaact gtaagtgctc ttttgattct ctttcactaa
   119461 agaccgaaca gttatctgat ttttgatggg caaaatgagc acattttttc tttccctttt
   119521 tcaaaatcac tctttttcga cagccacgac aataatattt ctttcctttt tctgcatgtt
   119581 tagcaaaaca gcacttatta tattcattca ttgcaagcag catttttctc cctctgaact
   119641 tatttacgtc taagaattta tttacacgaa aaaagagggc gtgaaaaagc tattctgcat
   119701 caagtaataa gaacagctaa acaaaaatag ttcccaatat ttttcgtttg gttgggcttg
   119761 ttaccgcaga tgcaagcttt tgaacacatt tatacgaaaa aaagactgcg acacaatttt
   119821 tgtcacagtc tcttactata atgctgattt atacaaaata aaatttcgtt cgctcgatag
   119881 cattacgcgc catcagacat tgtccatatt cattcaatgt ttctggtgta cgatcgctct
   119941 tttcagcaaa ctccaaaatt cgagcaatct gattctctac atccgcttgg ttgagatttt
   120001 ccatccagaa gtgaacgact aggtaatagc gctcttcgaa taaatacaaa tcagtccaag
   120061 cagactctag ttcgacatct gttgcaagtt ggatcacttg ttcaaaatct tcaaaagcaa
   120121 aagttgcttg tctttcctgt tcttctaggg cgcttccaga taattcttcc tccggatcgc
   120181 tgccaataat ctgctgacga agaaagtcac tcacatcttc tgaatcaaca gtagaatctt
   120241 ctgattgaga aatttcttca tccggaagat ttttactgat aaacagttct aatccgtctc
   120301 cttttggcaa aacttggaaa gtgacagctt cgcttcctcg gaactcctct tcgatatcaa
   120361 cttcttccaa aatactataa aagaaatttt cgatttcttt gtgattgcca agtaaatcca
   120421 aaaatgaaac gccccgttcc tctaaatctt catgaccaat tagtacacgg atcgtatttt
   120481 cattaatatg ttccatttcc attttgctac acctcacttt gccacaatgt ttaatataga
   120541 ttcattgtag cgaaatcagc ttaaatgtaa agaaaaaaaa tctcgctgtt aagcgaattt
   120601 aataaaacca gcggattttt caacctttca agctatctta tttaactata acatttttgg
   120661 cagacgaata ctatatagcc gacttttcag ctaattttcc ctctctattt ttcgtgcaat
   120721 catcatcttc tactaccttt ttatacatct aatcaagaat actttttgta aaaacaaaaa
   120781 aacttggaaa aactatcttt gtaatagtct tt
//
LOCUS       DAFCHQ010000002       104233 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000002 DAFCHQ010000000
VERSION     DAFCHQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 104233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.002"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            212..604
                     /locus_tag="LNC71_000117"
     CDS             212..604
                     /locus_tag="LNC71_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6465217.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="LNC71_000118"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="LNC71_000118"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBK6465218.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="LNC71_000119"
     CDS             3165..4775
                     /locus_tag="LNC71_000119"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBK6465219.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="LNC71_000120"
     CDS             5067..5936
                     /locus_tag="LNC71_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBK6465220.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="LNC71_000121"
     CDS             6101..7372
                     /locus_tag="LNC71_000121"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK6465221.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="LNC71_000122"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="LNC71_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBK6465222.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="LNC71_000123"
     CDS             8763..9026
                     /locus_tag="LNC71_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBK6465223.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="LNC71_000124"
     CDS             9471..9920
                     /locus_tag="LNC71_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6465224.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="LNC71_000125"
     CDS             9977..11395
                     /locus_tag="LNC71_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6465225.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="LNC71_000126"
     CDS             11395..12255
                     /locus_tag="LNC71_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBK6465226.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="LNC71_000127"
     CDS             12429..13202
                     /locus_tag="LNC71_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6465227.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="LNC71_000128"
     CDS             13349..13714
                     /locus_tag="LNC71_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBK6465228.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="LNC71_000129"
     CDS             complement(13862..15070)
                     /locus_tag="LNC71_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6465229.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="LNC71_000130"
     CDS             complement(15089..15367)
                     /locus_tag="LNC71_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465230.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="LNC71_000131"
     CDS             15521..16216
                     /locus_tag="LNC71_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6465231.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="LNC71_000132"
     CDS             complement(16658..17455)
                     /locus_tag="LNC71_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBK6465232.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="LNC71_000133"
     CDS             complement(17452..18636)
                     /locus_tag="LNC71_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK6465233.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="LNC71_000134"
     CDS             complement(18661..19908)
                     /locus_tag="LNC71_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK6465234.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="LNC71_000135"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="LNC71_000135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBK6465235.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="LNC71_000136"
     CDS             21888..22202
                     /locus_tag="LNC71_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBK6465236.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="LNC71_000137"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="LNC71_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBK6465237.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="LNC71_000138"
     CDS             22887..23531
                     /locus_tag="LNC71_000138"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBK6465238.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="LNC71_000139"
     CDS             23546..23875
                     /locus_tag="LNC71_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBK6465239.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="LNC71_000140"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="LNC71_000140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBK6465240.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="LNC71_000141"
     CDS             24863..25687
                     /locus_tag="LNC71_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBK6465241.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="LNC71_000142"
     CDS             25680..26027
                     /locus_tag="LNC71_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBK6465242.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="LNC71_000143"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="LNC71_000143"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBK6465243.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="LNC71_000144"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="LNC71_000144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBK6465244.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LNC71_000145"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LNC71_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBK6465245.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LNC71_000146"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LNC71_000146"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBK6465246.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="LNC71_000147"
     CDS             31657..32451
                     /locus_tag="LNC71_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465247.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="LNC71_000148"
     CDS             32441..34483
                     /locus_tag="LNC71_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465248.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="LNC71_000149"
     CDS             34764..34913
                     /locus_tag="LNC71_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBK6465249.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="LNC71_000150"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="LNC71_000150"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBK6465250.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="LNC71_000151"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="LNC71_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBK6465251.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="LNC71_000152"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="LNC71_000152"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBK6465252.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="LNC71_000153"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="LNC71_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBK6465253.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="LNC71_000154"
     CDS             39330..40412
                     /locus_tag="LNC71_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBK6465254.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="LNC71_000155"
     CDS             40412..41215
                     /locus_tag="LNC71_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK6465255.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="LNC71_000156"
     CDS             41285..42025
                     /locus_tag="LNC71_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6465256.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="LNC71_000157"
     CDS             42036..43040
                     /locus_tag="LNC71_000157"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBK6465257.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="LNC71_000158"
     CDS             complement(43085..44929)
                     /locus_tag="LNC71_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465258.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45140..45658
                     /locus_tag="LNC71_000159"
     CDS             45140..45658
                     /locus_tag="LNC71_000159"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6465259.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45786..46469
                     /locus_tag="LNC71_000160"
     CDS             45786..46469
                     /locus_tag="LNC71_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBK6465260.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46778..46851
                     /locus_tag="LNC71_000161"
     tRNA            46778..46851
                     /locus_tag="LNC71_000161"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46812..46814,aa:Arg,seq:tct)
     gene            47010..47369
                     /locus_tag="LNC71_000162"
     CDS             47010..47369
                     /locus_tag="LNC71_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBK6465261.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47492..49123
                     /locus_tag="LNC71_000163"
     CDS             47492..49123
                     /locus_tag="LNC71_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBK6465262.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49185..49826)
                     /locus_tag="LNC71_000164"
     CDS             complement(49185..49826)
                     /locus_tag="LNC71_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK6465263.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50012..50088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50108..50416
                     /gene="rplU"
                     /locus_tag="LNC71_000165"
     CDS             50108..50416
                     /gene="rplU"
                     /locus_tag="LNC71_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBK6465264.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50443..50787
                     /locus_tag="LNC71_000166"
     CDS             50443..50787
                     /locus_tag="LNC71_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBK6465265.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50800..51093
                     /gene="rpmA"
                     /locus_tag="LNC71_000167"
     CDS             50800..51093
                     /gene="rpmA"
                     /locus_tag="LNC71_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBK6465266.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51527..54334
                     /locus_tag="LNC71_000168"
     CDS             51527..54334
                     /locus_tag="LNC71_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6465267.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54525..55016
                     /locus_tag="LNC71_000169"
     CDS             54525..55016
                     /locus_tag="LNC71_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6465268.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55119..56108
                     /gene="manX"
                     /locus_tag="LNC71_000170"
     CDS             55119..56108
                     /gene="manX"
                     /locus_tag="LNC71_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBK6465269.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56141..56944
                     /locus_tag="LNC71_000171"
     CDS             56141..56944
                     /locus_tag="LNC71_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBK6465270.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56965..57879
                     /locus_tag="LNC71_000172"
     CDS             56965..57879
                     /locus_tag="LNC71_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBK6465271.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57962..58336
                     /locus_tag="LNC71_000173"
     CDS             57962..58336
                     /locus_tag="LNC71_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBK6465272.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58475..58720
                     /locus_tag="LNC71_000174"
     CDS             58475..58720
                     /locus_tag="LNC71_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6465273.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58892..59956
                     /locus_tag="LNC71_000175"
     CDS             58892..59956
                     /locus_tag="LNC71_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK6465274.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60202..60627
                     /locus_tag="LNC71_000176"
     CDS             60202..60627
                     /locus_tag="LNC71_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6465275.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60620..61069
                     /gene="nusB"
                     /locus_tag="LNC71_000177"
     CDS             60620..61069
                     /gene="nusB"
                     /locus_tag="LNC71_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBK6465276.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61166..62020
                     /locus_tag="LNC71_000178"
     CDS             61166..62020
                     /locus_tag="LNC71_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBK6465277.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62021..63358
                     /locus_tag="LNC71_000179"
     CDS             62021..63358
                     /locus_tag="LNC71_000179"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBK6465278.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63363..63587
                     /locus_tag="LNC71_000180"
     CDS             63363..63587
                     /locus_tag="LNC71_000180"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBK6465279.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63589..64479
                     /locus_tag="LNC71_000181"
     CDS             63589..64479
                     /locus_tag="LNC71_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK6465280.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64488..65306
                     /locus_tag="LNC71_000182"
     CDS             64488..65306
                     /locus_tag="LNC71_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBK6465281.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65413..65862
                     /locus_tag="LNC71_000183"
     CDS             65413..65862
                     /locus_tag="LNC71_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK6465282.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65880..67562
                     /gene="recN"
                     /locus_tag="LNC71_000184"
     CDS             65880..67562
                     /gene="recN"
                     /locus_tag="LNC71_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBK6465283.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67617..67733)
                     /locus_tag="LNC71_000185"
     CDS             complement(67617..67733)
                     /locus_tag="LNC71_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBK6465284.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67806..68762)
                     /locus_tag="LNC71_000186"
     CDS             complement(67806..68762)
                     /locus_tag="LNC71_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6465285.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68933..69301
                     /locus_tag="LNC71_000187"
     CDS             68933..69301
                     /locus_tag="LNC71_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBK6465286.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69471..69902
                     /gene="mraZ"
                     /locus_tag="LNC71_000188"
     CDS             69471..69902
                     /gene="mraZ"
                     /locus_tag="LNC71_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBK6465287.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69923..70882
                     /gene="rsmH"
                     /locus_tag="LNC71_000189"
     CDS             69923..70882
                     /gene="rsmH"
                     /locus_tag="LNC71_000189"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBK6465288.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70887..71288
                     /gene="ftsL"
                     /locus_tag="LNC71_000190"
     CDS             70887..71288
                     /gene="ftsL"
                     /locus_tag="LNC71_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBK6465289.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71289..73481
                     /locus_tag="LNC71_000191"
     CDS             71289..73481
                     /locus_tag="LNC71_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK6465290.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73507..74469
                     /locus_tag="LNC71_000192"
     CDS             73507..74469
                     /locus_tag="LNC71_000192"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBK6465291.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74483..75844
                     /locus_tag="LNC71_000193"
     CDS             74483..75844
                     /locus_tag="LNC71_000193"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBK6465292.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75870..76958
                     /gene="murG"
                     /locus_tag="LNC71_000194"
     CDS             75870..76958
                     /gene="murG"
                     /locus_tag="LNC71_000194"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBK6465293.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76973..78193
                     /locus_tag="LNC71_000195"
     CDS             76973..78193
                     /locus_tag="LNC71_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBK6465294.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78349..79674
                     /gene="ftsA"
                     /locus_tag="LNC71_000196"
     CDS             78349..79674
                     /gene="ftsA"
                     /locus_tag="LNC71_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBK6465295.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79697..80938
                     /gene="ftsZ"
                     /locus_tag="LNC71_000197"
     CDS             79697..80938
                     /gene="ftsZ"
                     /locus_tag="LNC71_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBK6465296.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80955..81632
                     /locus_tag="LNC71_000198"
     CDS             80955..81632
                     /locus_tag="LNC71_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBK6465297.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81646..82245
                     /locus_tag="LNC71_000199"
     CDS             81646..82245
                     /locus_tag="LNC71_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBK6465298.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82260..82547
                     /locus_tag="LNC71_000200"
     CDS             82260..82547
                     /locus_tag="LNC71_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBK6465299.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82596..83378
                     /locus_tag="LNC71_000201"
     CDS             82596..83378
                     /locus_tag="LNC71_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBK6465300.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83399..84106
                     /locus_tag="LNC71_000202"
     CDS             83399..84106
                     /locus_tag="LNC71_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBK6465301.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84368..87166
                     /gene="ileS"
                     /locus_tag="LNC71_000203"
     CDS             84368..87166
                     /gene="ileS"
                     /locus_tag="LNC71_000203"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBK6465302.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87215..88741)
                     /locus_tag="LNC71_000204"
     CDS             complement(87215..88741)
                     /locus_tag="LNC71_000204"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBK6465303.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88756..89403)
                     /locus_tag="LNC71_000205"
     CDS             complement(88756..89403)
                     /locus_tag="LNC71_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBK6465304.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89587..89916)
                     /locus_tag="LNC71_000206"
     CDS             complement(89587..89916)
                     /locus_tag="LNC71_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBK6465305.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90093..90821
                     /gene="sfsA"
                     /locus_tag="LNC71_000207"
     CDS             90093..90821
                     /gene="sfsA"
                     /locus_tag="LNC71_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBK6465306.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90837..91850
                     /locus_tag="LNC71_000208"
     CDS             90837..91850
                     /locus_tag="LNC71_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6465307.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91850..93127
                     /locus_tag="LNC71_000209"
     CDS             91850..93127
                     /locus_tag="LNC71_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6465308.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93190..95892
                     /locus_tag="LNC71_000210"
     CDS             93190..95892
                     /locus_tag="LNC71_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6465309.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96044..96361
                     /locus_tag="LNC71_000211"
     CDS             96044..96361
                     /locus_tag="LNC71_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6465310.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96391..96711
                     /locus_tag="LNC71_000212"
     CDS             96391..96711
                     /locus_tag="LNC71_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6465311.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96819..98279
                     /locus_tag="LNC71_000213"
     CDS             96819..98279
                     /locus_tag="LNC71_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6465312.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98347..98568
                     /locus_tag="LNC71_000214"
     CDS             98347..98568
                     /locus_tag="LNC71_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465313.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98599..98781
                     /locus_tag="LNC71_000215"
     CDS             98599..98781
                     /locus_tag="LNC71_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBK6465314.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98781..99194
                     /locus_tag="LNC71_000216"
     CDS             98781..99194
                     /locus_tag="LNC71_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBK6465315.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99317..100498
                     /locus_tag="LNC71_000217"
     CDS             99317..100498
                     /locus_tag="LNC71_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6465316.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100569..100733)
                     /locus_tag="LNC71_000218"
     CDS             complement(100569..100733)
                     /locus_tag="LNC71_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465317.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100887..100960
                     /locus_tag="LNC71_000219"
     tRNA            100887..100960
                     /locus_tag="LNC71_000219"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100921..100923,aa:Arg,seq:cct)
     gene            complement(101029..102168)
                     /locus_tag="LNC71_000220"
     CDS             complement(101029..102168)
                     /locus_tag="LNC71_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6465318.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(102467..103102)
                     /locus_tag="LNC71_000221"
     CDS             complement(102467..103102)
                     /locus_tag="LNC71_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465319.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(103215..103850)
                     /locus_tag="LNC71_000222"
     CDS             complement(103215..103850)
                     /locus_tag="LNC71_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBK6465320.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(103884..>104233)
                     /locus_tag="LNC71_000223"
     CDS             complement(103884..>104233)
                     /locus_tag="LNC71_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBK6465321.1"
                     /translation="GEKTIPFKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATK
                     DENTVMFTKKYWNEMENLKKYIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQ
                     EEFDFKKKELLGL"
BASE COUNT        33923 a        17834 c        21902 g        30574 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaatttat cgttattata tgattaagaa ggattgaata
    45121 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    45181 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    45241 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    45301 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    45361 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    45421 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    45481 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    45541 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    45601 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    45661 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    45721 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    45781 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    45841 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    45901 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    45961 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    46021 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    46081 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    46141 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    46201 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    46261 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    46321 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    46381 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    46441 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    46501 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    46561 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    46621 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    46681 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    46741 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    46801 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    46861 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    46921 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    46981 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    47041 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    47101 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    47161 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    47221 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    47281 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    47341 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    47401 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    47461 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    47521 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    47581 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    47641 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    47701 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    47761 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    47821 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    47881 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    47941 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    48001 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    48061 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    48121 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    48181 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    48241 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    48301 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    48361 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    48421 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    48481 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
    48541 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    48601 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    48661 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    48721 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    48781 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    48841 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    48901 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    48961 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    49021 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    49081 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    49141 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    49201 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    49261 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    49321 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    49381 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    49441 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    49501 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    49561 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    49621 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    49681 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    49741 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    49801 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    49861 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    49921 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    49981 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    50041 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    50101 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    50161 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    50221 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    50281 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    50341 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    50401 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    50461 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    50521 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    50581 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    50641 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    50701 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    50761 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    50821 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    50881 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    50941 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    51001 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    51061 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    51121 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    51181 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    51241 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    51301 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    51361 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    51421 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    51481 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    51541 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    51601 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    51661 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    51721 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    51781 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    51841 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    51901 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    51961 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    52021 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    52081 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    52141 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    52201 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    52261 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    52321 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    52381 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    52441 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    52501 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    52561 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    52621 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    52681 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    52741 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    52801 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    52861 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    52921 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    52981 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    53041 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    53101 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    53161 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    53221 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    53281 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    53341 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    53401 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    53461 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    53521 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    53581 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    53641 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    53701 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    53761 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    53821 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    53881 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    53941 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    54001 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    54061 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    54121 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    54181 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    54241 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    54301 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    54361 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    54421 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    54481 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    54541 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    54601 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    54661 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    54721 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    54781 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    54841 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    54901 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    54961 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    55021 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    55081 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    55141 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    55201 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    55261 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    55321 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    55381 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    55441 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    55501 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    55561 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    55621 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    55681 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    55741 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    55801 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    55861 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    55921 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    55981 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    56041 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    56101 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    56161 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    56221 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    56281 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    56341 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    56401 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    56461 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    56521 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    56581 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    56641 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    56701 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    56761 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    56821 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    56881 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    56941 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    57001 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    57061 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    57121 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    57181 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    57241 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    57301 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    57361 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    57421 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    57481 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    57541 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    57601 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    57661 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    57721 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    57781 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    57841 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    57901 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    57961 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    58021 tctgacagat tacgggcaaa tcatgatagg taataatggt tttgagtttt accatgcgcg
    58081 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    58141 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    58201 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    58261 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    58321 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    58381 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    58441 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    58501 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    58561 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    58621 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    58681 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    58741 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    58801 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    58861 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    58921 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    58981 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    59041 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    59101 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    59161 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    59221 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    59281 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    59341 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    59401 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    59461 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    59521 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    59581 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    59641 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    59701 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    59761 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    59821 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    59881 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    59941 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    60001 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    60061 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    60121 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    60181 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    60241 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    60301 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    60361 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    60421 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    60481 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    60541 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    60601 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    60661 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    60721 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    60781 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    60841 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    60901 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    60961 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    61021 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    61081 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    61141 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    61201 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    61261 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    61321 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    61381 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    61441 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    61501 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    61561 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    61621 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    61681 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    61741 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    61801 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    61861 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    61921 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    61981 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    62041 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    62101 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    62161 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    62221 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    62281 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    62341 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    62401 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    62461 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    62521 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    62581 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    62641 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    62701 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    62761 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    62821 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    62881 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    62941 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    63001 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    63061 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    63121 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    63181 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    63241 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    63301 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    63361 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    63421 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    63481 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    63541 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    63601 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    63661 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    63721 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    63781 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    63841 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    63901 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    63961 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    64021 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    64081 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    64141 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    64201 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    64261 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    64321 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    64381 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    64441 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    64501 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    64561 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    64621 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    64681 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    64741 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    64801 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    64861 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    64921 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    64981 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    65041 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    65101 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    65161 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    65221 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    65281 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    65341 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    65401 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    65461 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    65521 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    65581 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    65641 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    65701 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    65761 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    65821 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    65881 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    65941 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    66001 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    66061 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    66121 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    66181 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    66241 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    66301 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    66361 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    66421 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    66481 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    66541 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    66601 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    66661 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    66721 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    66781 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    66841 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    66901 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    66961 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    67021 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    67081 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    67141 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    67201 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    67261 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    67321 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    67381 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    67441 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    67501 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    67561 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    67621 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    67681 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    67741 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    67801 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    67861 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    67921 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    67981 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    68041 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    68101 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    68161 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    68221 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    68281 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    68341 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    68401 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    68461 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    68521 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    68581 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    68641 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    68701 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    68761 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    68821 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    68881 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    68941 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    69001 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    69061 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    69121 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    69181 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    69241 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    69301 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    69361 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    69421 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    69481 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    69541 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    69601 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    69661 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    69721 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    69781 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    69841 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    69901 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    69961 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    70021 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    70081 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    70141 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    70201 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    70261 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    70321 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    70381 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    70441 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    70501 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    70561 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    70621 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    70681 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    70741 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    70801 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    70861 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    70921 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    70981 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    71041 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    71101 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    71161 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    71221 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    71281 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    71341 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    71401 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    71461 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    71521 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    71581 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    71641 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    71701 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    71761 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    71821 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    71881 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    71941 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    72001 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    72061 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    72121 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    72181 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    72241 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    72301 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    72361 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    72421 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    72481 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    72541 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    72601 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    72661 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    72721 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    72781 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    72841 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    72901 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    72961 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    73021 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    73081 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    73141 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    73201 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    73261 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    73321 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    73381 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    73441 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    73501 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    73561 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    73621 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    73681 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    73741 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    73801 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    73861 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    73921 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    73981 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    74041 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    74101 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    74161 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    74221 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    74281 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    74341 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    74401 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    74461 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    74521 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    74581 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    74641 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    74701 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    74761 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    74821 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    74881 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    74941 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    75001 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    75061 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    75121 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    75181 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    75241 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    75301 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    75361 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    75421 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    75481 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    75541 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    75601 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    75661 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    75721 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    75781 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    75841 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    75901 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    75961 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    76021 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    76081 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    76141 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    76201 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    76261 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    76321 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    76381 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    76441 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    76501 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    76561 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    76621 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    76681 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    76741 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    76801 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    76861 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    76921 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    76981 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    77041 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    77101 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    77161 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    77221 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    77281 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    77341 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    77401 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    77461 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    77521 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    77581 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    77641 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    77701 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    77761 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    77821 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    77881 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    77941 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    78001 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    78061 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    78121 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    78181 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    78241 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    78301 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    78361 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    78421 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    78481 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    78541 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    78601 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    78661 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    78721 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    78781 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    78841 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    78901 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    78961 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    79021 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    79081 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    79141 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    79201 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    79261 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    79321 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    79381 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    79441 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    79501 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    79561 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    79621 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    79681 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    79741 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    79801 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    79861 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    79921 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    79981 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    80041 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    80101 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    80161 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    80221 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    80281 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    80341 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    80401 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    80461 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    80521 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    80581 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    80641 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    80701 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    80761 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    80821 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    80881 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    80941 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    81001 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    81061 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    81121 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    81181 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    81241 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    81301 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    81361 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    81421 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    81481 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    81541 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    81601 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    81661 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    81721 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    81781 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    81841 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    81901 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    81961 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    82021 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    82081 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    82141 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    82201 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    82261 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    82321 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    82381 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    82441 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    82501 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    82561 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    82621 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    82681 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    82741 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    82801 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    82861 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    82921 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    82981 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    83041 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    83101 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    83161 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    83221 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    83281 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    83341 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    83401 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    83461 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    83521 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    83581 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    83641 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    83701 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    83761 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    83821 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    83881 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    83941 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    84001 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    84061 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    84121 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    84181 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    84241 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    84301 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    84361 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    84421 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    84481 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    84541 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    84601 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    84661 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    84721 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    84781 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    84841 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    84901 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    84961 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    85021 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    85081 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    85141 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    85201 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    85261 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    85321 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    85381 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    85441 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    85501 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    85561 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    85621 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    85681 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    85741 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    85801 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    85861 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    85921 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    85981 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    86041 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    86101 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    86161 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    86221 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    86281 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    86341 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    86401 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    86461 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    86521 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    86581 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    86641 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    86701 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    86761 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    86821 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    86881 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    86941 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    87001 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    87061 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    87121 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    87181 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    87241 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    87301 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    87361 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    87421 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    87481 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    87541 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    87601 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    87661 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    87721 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    87781 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    87841 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    87901 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    87961 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    88021 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    88081 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    88141 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    88201 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    88261 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    88321 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    88381 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    88441 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    88501 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    88561 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    88621 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    88681 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    88741 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    88801 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    88861 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    88921 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    88981 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    89041 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    89101 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    89161 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    89221 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    89281 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    89341 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    89401 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    89461 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    89521 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    89581 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    89641 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    89701 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    89761 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    89821 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    89881 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    89941 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    90001 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    90061 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    90121 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    90181 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    90241 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    90301 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    90361 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    90421 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    90481 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    90541 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    90601 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    90661 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    90721 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    90781 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    90841 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    90901 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    90961 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    91021 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    91081 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    91141 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    91201 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    91261 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    91321 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    91381 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    91441 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    91501 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    91561 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    91621 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    91681 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    91741 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    91801 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    91861 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    91921 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    91981 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    92041 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    92101 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    92161 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    92221 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    92281 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    92341 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    92401 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    92461 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    92521 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    92581 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    92641 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    92701 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    92761 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    92821 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    92881 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    92941 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    93001 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    93061 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    93121 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    93181 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    93241 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    93301 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    93361 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    93421 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    93481 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    93541 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    93601 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgatcaaaa tccgcaactg
    93661 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    93721 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    93781 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    93841 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    93901 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    93961 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    94021 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    94081 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    94141 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    94201 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    94261 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    94321 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    94381 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    94441 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    94501 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    94561 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    94621 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    94681 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    94741 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    94801 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    94861 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    94921 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    94981 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    95041 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    95101 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    95161 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    95221 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    95281 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    95341 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    95401 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    95461 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    95521 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    95581 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    95641 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    95701 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    95761 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    95821 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    95881 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    95941 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    96001 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    96061 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    96121 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    96181 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    96241 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    96301 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    96361 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    96421 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    96481 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    96541 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    96601 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    96661 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    96721 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    96781 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    96841 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    96901 tgcgtgatgc ttttatcggc atactgcccg caacaatggc gggatcagtc gctgtaatga
    96961 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    97021 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    97081 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    97141 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    97201 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    97261 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    97321 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    97381 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    97441 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    97501 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    97561 ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc
    97621 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    97681 ttcatggatc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    97741 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    97801 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    97861 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    97921 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    97981 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    98041 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    98101 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    98161 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    98221 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    98281 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    98341 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    98401 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    98461 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    98521 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    98581 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    98641 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    98701 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    98761 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    98821 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    98881 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    98941 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    99001 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    99061 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    99121 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    99181 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    99241 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    99301 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    99361 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    99421 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    99481 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    99541 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    99601 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    99661 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    99721 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    99781 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    99841 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    99901 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    99961 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
   100021 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
   100081 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
   100141 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
   100201 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
   100261 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
   100321 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
   100381 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
   100441 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
   100501 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
   100561 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
   100621 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
   100681 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
   100741 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
   100801 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
   100861 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
   100921 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc
   100981 tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt
   101041 tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg
   101101 aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
   101161 tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat gccttagtcc
   101221 atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca aatatttgct
   101281 gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat tatgagggtt
   101341 tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt ttaagagatc
   101401 aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg tttttcccca
   101461 ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt cgaaatcgat
   101521 atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac cagcaactaa
   101581 cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt tcattaaaga
   101641 tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat cttttccttt
   101701 gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg catctttcaa
   101761 cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt ctttagcata
   101821 cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata ccgctggcat
   101881 atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag gagatactgt
   101941 tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata gatttttgcg
   102001 atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat cttttgcttc
   102061 tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat cttttttgta
   102121 tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa ttgaatcagc
   102181 tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt ttattatttt
   102241 ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt aatggtataa
   102301 ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt gatttttaaa
   102361 agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt ctggtttttt
   102421 attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa gtaagagttg
   102481 agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt ttgccgacat
   102541 cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat aatctattta
   102601 agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca gtgaaatttt
   102661 cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt aattgattca
   102721 gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga agtaaacaat
   102781 tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt tctacctcgt
   102841 catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg aaaacatctt
   102901 ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag aatttctcga
   102961 tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca acgccaccaa
   103021 tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct tttttggtta
   103081 gtttttcatt gctattatcc atagttacct cctattttgt catttccttt acaagcattg
   103141 tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct aactcttagt
   103201 ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca ttgagcatag
   103261 gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac tttatcgccc
   103321 tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga aactttaggt
   103381 gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat aatggctatt
   103441 ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc actaatatat
   103501 ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact atctaagaaa
   103561 gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag gcttttattg
   103621 ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt gttattgtca
   103681 tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat cccgataata
   103741 aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat taacatgatt
   103801 aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat taatagatcc
   103861 tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt aaaatcgaat
   103921 tcttcttgat taataatccc ttcatctaac aactctttat actttttaat ttcatctgca
   103981 cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt ctctatatat
   104041 ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt gttttcatct
   104101 ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt aaattgtata
   104161 taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt aaaggggata
   104221 gttttttctc ctt
//
LOCUS       DAFCHQ010000003        87643 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000003 DAFCHQ010000000
VERSION     DAFCHQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.003"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(317..1813)
                     /gene="lysS"
                     /locus_tag="LNC71_000224"
     CDS             complement(317..1813)
                     /gene="lysS"
                     /locus_tag="LNC71_000224"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBK6465322.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="LNC71_000225"
     CDS             complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="LNC71_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBK6465323.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="LNC71_000226"
     CDS             complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="LNC71_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBK6465324.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="LNC71_000227"
     CDS             complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="LNC71_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBK6465325.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="LNC71_000228"
     CDS             complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="LNC71_000228"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBK6465326.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="LNC71_000229"
     CDS             complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="LNC71_000229"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBK6465327.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8344..8814)
                     /locus_tag="LNC71_000230"
     CDS             complement(8344..8814)
                     /locus_tag="LNC71_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBK6465328.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8860..9285)
                     /locus_tag="LNC71_000231"
     CDS             complement(8860..9285)
                     /locus_tag="LNC71_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBK6465329.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9352..9621)
                     /locus_tag="LNC71_000232"
     CDS             complement(9352..9621)
                     /locus_tag="LNC71_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBK6465330.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9632..11227)
                     /locus_tag="LNC71_000233"
     CDS             complement(9632..11227)
                     /locus_tag="LNC71_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6465331.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="LNC71_000234"
     CDS             complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="LNC71_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBK6465332.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14780..15340)
                     /locus_tag="LNC71_000235"
     CDS             complement(14780..15340)
                     /locus_tag="LNC71_000235"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBK6465333.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15693..16667
                     /locus_tag="LNC71_000236"
     CDS             15693..16667
                     /locus_tag="LNC71_000236"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK6465334.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16837..18237
                     /locus_tag="LNC71_000237"
     CDS             16837..18237
                     /locus_tag="LNC71_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBK6465335.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18315..19037)
                     /locus_tag="LNC71_000238"
     CDS             complement(18315..19037)
                     /locus_tag="LNC71_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6465336.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19116..19622)
                     /locus_tag="LNC71_000239"
     CDS             complement(19116..19622)
                     /locus_tag="LNC71_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6465337.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19745..21544
                     /locus_tag="LNC71_000240"
     CDS             19745..21544
                     /locus_tag="LNC71_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBK6465338.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21612..23222)
                     /locus_tag="LNC71_000241"
     CDS             complement(21612..23222)
                     /locus_tag="LNC71_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK6465339.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTG
                     SSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCS
                     GFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGG
                     QYIHAPQPGESVKVGSVQWFAPDFAVSM"
     gene            complement(23477..23986)
                     /gene="mreD"
                     /locus_tag="LNC71_000242"
     CDS             complement(23477..23986)
                     /gene="mreD"
                     /locus_tag="LNC71_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBK6465340.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23990..24844)
                     /gene="mreC"
                     /locus_tag="LNC71_000243"
     CDS             complement(23990..24844)
                     /gene="mreC"
                     /locus_tag="LNC71_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBK6465341.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24999..25637)
                     /locus_tag="LNC71_000244"
     CDS             complement(24999..25637)
                     /locus_tag="LNC71_000244"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBK6465342.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25638..26288)
                     /locus_tag="LNC71_000245"
     CDS             complement(25638..26288)
                     /locus_tag="LNC71_000245"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBK6465343.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26301..27200)
                     /gene="rsgA"
                     /locus_tag="LNC71_000246"
     CDS             complement(26301..27200)
                     /gene="rsgA"
                     /locus_tag="LNC71_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBK6465344.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27365..29434)
                     /gene="pknB"
                     /locus_tag="LNC71_000247"
     CDS             complement(27365..29434)
                     /gene="pknB"
                     /locus_tag="LNC71_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBK6465345.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29431..30171)
                     /locus_tag="LNC71_000248"
     CDS             complement(29431..30171)
                     /locus_tag="LNC71_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBK6465346.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30184..31542)
                     /gene="rsmB"
                     /locus_tag="LNC71_000249"
     CDS             complement(30184..31542)
                     /gene="rsmB"
                     /locus_tag="LNC71_000249"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBK6465347.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31542..32480)
                     /locus_tag="LNC71_000250"
     CDS             complement(31542..32480)
                     /locus_tag="LNC71_000250"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBK6465348.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32473..32964)
                     /gene="def"
                     /locus_tag="LNC71_000251"
     CDS             complement(32473..32964)
                     /gene="def"
                     /locus_tag="LNC71_000251"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK6465349.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32979..35387)
                     /gene="priA"
                     /locus_tag="LNC71_000252"
     CDS             complement(32979..35387)
                     /gene="priA"
                     /locus_tag="LNC71_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBK6465350.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35640..35945)
                     /locus_tag="LNC71_000253"
     CDS             complement(35640..35945)
                     /locus_tag="LNC71_000253"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK6465351.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35950..36564)
                     /gene="gmk"
                     /locus_tag="LNC71_000254"
     CDS             complement(35950..36564)
                     /gene="gmk"
                     /locus_tag="LNC71_000254"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBK6465352.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37051..37335)
                     /locus_tag="LNC71_000255"
     CDS             complement(37051..37335)
                     /locus_tag="LNC71_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465353.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37353..38243)
                     /locus_tag="LNC71_000256"
     CDS             complement(37353..38243)
                     /locus_tag="LNC71_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBK6465354.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38263..38481)
                     /locus_tag="LNC71_000257"
     CDS             complement(38263..38481)
                     /locus_tag="LNC71_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBK6465355.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38451..40151)
                     /locus_tag="LNC71_000258"
     CDS             complement(38451..40151)
                     /locus_tag="LNC71_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBK6465356.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40418..41191
                     /locus_tag="LNC71_000259"
     CDS             40418..41191
                     /locus_tag="LNC71_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465357.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41272..41841)
                     /gene="thiT"
                     /locus_tag="LNC71_000260"
     CDS             complement(41272..41841)
                     /gene="thiT"
                     /locus_tag="LNC71_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBK6465358.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41907..42009)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(42015..42986)
                     /locus_tag="LNC71_000261"
     CDS             complement(42015..42986)
                     /locus_tag="LNC71_000261"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK6465359.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(43080..44453)
                     /gene="glmU"
                     /locus_tag="LNC71_000262"
     CDS             complement(43080..44453)
                     /gene="glmU"
                     /locus_tag="LNC71_000262"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBK6465360.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44956..45774)
                     /gene="purR"
                     /locus_tag="LNC71_000263"
     CDS             complement(44956..45774)
                     /gene="purR"
                     /locus_tag="LNC71_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBK6465361.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45941..46765)
                     /locus_tag="LNC71_000264"
     CDS             complement(45941..46765)
                     /locus_tag="LNC71_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK6465362.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46738..47418)
                     /locus_tag="LNC71_000265"
     CDS             complement(46738..47418)
                     /locus_tag="LNC71_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK6465363.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47441..48379)
                     /locus_tag="LNC71_000266"
     CDS             complement(47441..48379)
                     /locus_tag="LNC71_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBK6465364.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48624..49487)
                     /locus_tag="LNC71_000267"
     CDS             complement(48624..49487)
                     /locus_tag="LNC71_000267"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBK6465365.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49629..52202)
                     /gene="mprF"
                     /locus_tag="LNC71_000268"
     CDS             complement(49629..52202)
                     /gene="mprF"
                     /locus_tag="LNC71_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK6465366.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52343..53149
                     /gene="proC"
                     /locus_tag="LNC71_000269"
     CDS             52343..53149
                     /gene="proC"
                     /locus_tag="LNC71_000269"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBK6465367.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(53228..54784)
                     /gene="rny"
                     /locus_tag="LNC71_000270"
     CDS             complement(53228..54784)
                     /gene="rny"
                     /locus_tag="LNC71_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBK6465368.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(55195..56244)
                     /gene="recA"
                     /locus_tag="LNC71_000271"
     CDS             complement(55195..56244)
                     /gene="recA"
                     /locus_tag="LNC71_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBK6465369.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56353..57594)
                     /locus_tag="LNC71_000272"
     CDS             complement(56353..57594)
                     /locus_tag="LNC71_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBK6465370.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57834..58412)
                     /gene="pgsA"
                     /locus_tag="LNC71_000273"
     CDS             complement(57834..58412)
                     /gene="pgsA"
                     /locus_tag="LNC71_000273"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBK6465371.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58621..59544)
                     /locus_tag="LNC71_000274"
     CDS             complement(58621..59544)
                     /locus_tag="LNC71_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6465372.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(60283..61569)
                     /locus_tag="LNC71_000275"
     CDS             complement(60283..61569)
                     /locus_tag="LNC71_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK6465373.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61562..62830)
                     /locus_tag="LNC71_000276"
     CDS             complement(61562..62830)
                     /locus_tag="LNC71_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBK6465374.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            63082..63543
                     /gene="mscL"
                     /locus_tag="LNC71_000277"
     CDS             63082..63543
                     /gene="mscL"
                     /locus_tag="LNC71_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBK6465375.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63602..64048)
                     /locus_tag="LNC71_000278"
     CDS             complement(63602..64048)
                     /locus_tag="LNC71_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6465376.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(64141..64386)
                     /locus_tag="LNC71_000279"
     CDS             complement(64141..64386)
                     /locus_tag="LNC71_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBK6465377.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64501..65079)
                     /locus_tag="LNC71_000280"
     CDS             complement(64501..65079)
                     /locus_tag="LNC71_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6465378.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(65156..65686)
                     /locus_tag="LNC71_000281"
     CDS             complement(65156..65686)
                     /locus_tag="LNC71_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBK6465379.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65690..66550)
                     /gene="rlmB"
                     /locus_tag="LNC71_000282"
     CDS             complement(65690..66550)
                     /gene="rlmB"
                     /locus_tag="LNC71_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBK6465380.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66543..66938)
                     /locus_tag="LNC71_000283"
     CDS             complement(66543..66938)
                     /locus_tag="LNC71_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBK6465381.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66935..68347)
                     /locus_tag="LNC71_000284"
     CDS             complement(66935..68347)
                     /locus_tag="LNC71_000284"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBK6465382.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68344..68880)
                     /gene="cysE"
                     /locus_tag="LNC71_000285"
     CDS             complement(68344..68880)
                     /gene="cysE"
                     /locus_tag="LNC71_000285"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBK6465383.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(68966..69205)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(69202..70662)
                     /locus_tag="LNC71_000286"
     CDS             complement(69202..70662)
                     /locus_tag="LNC71_000286"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBK6465384.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70832..71989)
                     /locus_tag="LNC71_000287"
     CDS             complement(70832..71989)
                     /locus_tag="LNC71_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBK6465385.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(72023..73393)
                     /gene="radA"
                     /locus_tag="LNC71_000288"
     CDS             complement(72023..73393)
                     /gene="radA"
                     /locus_tag="LNC71_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBK6465386.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73456..73983)
                     /locus_tag="LNC71_000289"
     CDS             complement(73456..73983)
                     /locus_tag="LNC71_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBK6465387.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            74213..75019
                     /gene="proB"
                     /locus_tag="LNC71_000290"
     CDS             74213..75019
                     /gene="proB"
                     /locus_tag="LNC71_000290"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBK6465388.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            75016..76263
                     /locus_tag="LNC71_000291"
     CDS             75016..76263
                     /locus_tag="LNC71_000291"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBK6465389.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76339..76683
                     /locus_tag="LNC71_000292"
     CDS             76339..76683
                     /locus_tag="LNC71_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465390.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76800..77126)
                     /locus_tag="LNC71_000293"
     CDS             complement(76800..77126)
                     /locus_tag="LNC71_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465391.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77741..78943)
                     /locus_tag="LNC71_000294"
     CDS             complement(77741..78943)
                     /locus_tag="LNC71_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6465392.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            79060..79575
                     /locus_tag="LNC71_000295"
     CDS             79060..79575
                     /locus_tag="LNC71_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6465393.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79774..80220)
                     /locus_tag="LNC71_000296"
     CDS             complement(79774..80220)
                     /locus_tag="LNC71_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK6465394.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(80267..81745)
                     /gene="msr(C)"
                     /locus_tag="LNC71_000297"
     CDS             complement(80267..81745)
                     /gene="msr(C)"
                     /locus_tag="LNC71_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK6465395.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(82099..82728)
                     /locus_tag="LNC71_000298"
     CDS             complement(82099..82728)
                     /locus_tag="LNC71_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6465396.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82712..83176)
                     /locus_tag="LNC71_000299"
     CDS             complement(82712..83176)
                     /locus_tag="LNC71_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6465397.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83663..84931)
                     /locus_tag="LNC71_000300"
     CDS             complement(83663..84931)
                     /locus_tag="LNC71_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6465398.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(85162..85899)
                     /locus_tag="LNC71_000301"
     CDS             complement(85162..85899)
                     /locus_tag="LNC71_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6465399.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85966..86535)
                     /locus_tag="LNC71_000302"
     CDS             complement(85966..86535)
                     /locus_tag="LNC71_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK6465400.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86663..87535
                     /locus_tag="LNC71_000303"
     CDS             86663..87535
                     /locus_tag="LNC71_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6465401.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT        24330 a        18931 c        15723 g        28659 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataacttttt tgttatgttt
       61 ttgaataaat attttcttct gtcttttgga caagtaataa tataccaact aaatttagct
      121 tggtcaacga attttttgta ttttttcaag tttttttcaa taaacgatta ttcataactt
      181 ttctttcttt atcgttcgtt ttcgtcttct ttaatgtcta gaatagtcgg cattagcttt
      241 tgaactttcg ttattattta ttcctttcta cacagcaaaa gctgcctaag gaattcctta
      301 gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga attgattgtg
      361 catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca gttggcggca
      421 taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg tcatttccta
      481 gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga tcgttcagtt
      541 ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga tctgtgaatc
      601 gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt ccgtatacaa
      661 acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg ataacatgtc
      721 cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct aatgcttctt
      781 ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca tctaccatat
      841 ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg atttttgctg
      901 ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc attacatcat
      961 tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta tgtgtagtat
     1021 caattccttc attacggaaa acgcggccga tttcataaac tttttccatt ccgccaacaa
     1081 ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg tccagtgcat
     1141 tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc aatactggtg
     1201 tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg ataatttggc
     1261 ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag tagcgttgtc
     1321 tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga cgaagtgctt
     1381 ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct gttttcatga
     1441 tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact tcataggcct
     1501 catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga tcttgtagat
     1561 gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct acactagcgg
     1621 tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca tgcaattctt
     1681 ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct tcacgcaatt
     1741 gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact tgatgttgtt
     1801 gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat aatgccaatg
     1861 aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg aaaaacttag
     1921 aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt gatccagcag
     1981 gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt tcgctgctcg
     2041 aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag ctactttttc
     2101 tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt tttcggctgg
     2161 tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt gaatcatcca
     2221 tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat agtcaagcat
     2281 ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa tcgtcaaatg
     2341 ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt
     2401 acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac
     2461 agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac
     2521 tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc
     2581 tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat ctattatatc
     2641 ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc
     2701 aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt
     2761 tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc
     2821 aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac
     2881 gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc
     2941 taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca
     3001 gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc
     3061 acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac
     3121 aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc
     3181 tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc
     3241 gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc
     3301 cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg
     3361 ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt
     3421 ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt
     3481 tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg
     3541 atttttgatg taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc
     3601 tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag
     3661 tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg
     3721 acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc
     3781 gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt
     3841 tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct
     3901 tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata
     3961 aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg
     4021 acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc
     4081 tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg
     4141 tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa
     4201 agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc
     4261 gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc
     4321 agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc
     4381 ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc
     4441 ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg
     4501 acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg
     4561 ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg
     4621 tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc
     4681 ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt
     4741 gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata
     4801 agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat
     4861 cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc
     4921 agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt
     4981 ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac
     5041 tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa
     5101 acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac
     5161 gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg
     5221 ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat
     5281 ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac
     5341 acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa
     5401 cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg
     5461 tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc
     5521 tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc
     5581 agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt
     5641 catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa
     5701 aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct
     5761 tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct
     5821 caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga
     5881 acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt
     5941 gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag
     6001 ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat
     6061 gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg catttttcat
     6121 catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt
     6181 tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt
     6241 gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca
     6301 taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct
     6361 tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt
     6421 gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata
     6481 tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt
     6541 acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc
     6601 aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca
     6661 cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg
     6721 caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc
     6781 aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat
     6841 cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc
     6901 ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga
     6961 taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt
     7021 ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag
     7081 accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt
     7141 ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct
     7201 ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta
     7261 tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa
     7321 tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa
     7381 aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa
     7441 atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt
     7501 gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct
     7561 gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat
     7621 ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat
     7681 ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt
     7741 gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca
     7801 ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt
     7861 ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt
     7921 tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc
     7981 gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac
     8041 ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat
     8101 caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat
     8161 tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt
     8221 tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac
     8281 aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc
     8341 tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga
     8401 agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt
     8461 ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc
     8521 acggggatct ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg
     8581 ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt
     8641 aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga
     8701 cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa
     8761 atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa
     8821 aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact
     8881 agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac
     8941 tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc
     9001 atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat
     9061 ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa
     9121 aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct
     9181 gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc
     9241 atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa
     9301 tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc
     9361 tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt
     9421 ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt
     9481 caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat
     9541 tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt
     9601 taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca
     9661 ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa
     9721 tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag
     9781 cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca
     9841 gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc
     9901 aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc
     9961 ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag
    10021 tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct
    10081 tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa
    10141 ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga
    10201 gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga
    10261 ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag
    10321 gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac
    10381 ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca
    10441 aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa
    10501 tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga
    10561 acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa
    10621 ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt
    10681 ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac
    10741 gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga
    10801 aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga
    10861 tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga
    10921 caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt
    10981 gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac
    11041 cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa
    11101 aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg
    11161 ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat
    11221 gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac
    11281 aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg
    11341 tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc
    11401 ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat
    11461 atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg
    11521 cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc
    11581 atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc
    11641 gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc
    11701 gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc
    11761 gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc
    11821 acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga
    11881 tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat
    11941 cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc
    12001 tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt
    12061 tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg
    12121 accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc
    12181 ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt
    12241 ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc
    12301 ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc
    12361 aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct
    12421 cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg
    12481 ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct
    12541 aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt
    12601 acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc
    12661 gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa
    12721 gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc
    12781 tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg
    12841 tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc
    12901 agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata
    12961 tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat
    13021 ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg
    13081 aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat
    13141 aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac
    13201 ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc
    13261 tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct
    13321 cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc
    13381 ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat
    13441 caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc
    13501 tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca
    13561 acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata
    13621 ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa
    13681 aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg
    13741 ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc
    13801 gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga
    13861 agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc
    13921 ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa
    13981 aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat
    14041 ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac
    14101 ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga
    14161 atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat
    14221 atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac
    14281 atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg
    14341 ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc
    14401 gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct
    14461 ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg
    14521 aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc
    14581 ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc
    14641 aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc
    14701 ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc
    14761 cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt
    14821 ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat
    14881 tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc
    14941 cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga
    15001 taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc actgccagat
    15061 ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg
    15121 ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt
    15181 ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt
    15241 gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt
    15301 atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc
    15361 taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc
    15421 ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa
    15481 tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa taattgatat
    15541 ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg
    15601 ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg
    15661 aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc
    15721 atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag
    15781 ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag
    15841 gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg
    15901 ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa
    15961 aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg
    16021 tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag
    16081 atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt
    16141 caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg
    16201 atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt
    16261 ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa
    16321 tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta
    16381 tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg
    16441 ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg
    16501 gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag
    16561 taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat
    16621 taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga
    16681 cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa
    16741 ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc
    16801 gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa
    16861 aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt
    16921 tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga
    16981 ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct
    17041 gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag
    17101 ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga
    17161 tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt
    17221 caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg
    17281 aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt
    17341 atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa
    17401 agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa
    17461 gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat
    17521 gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg
    17581 agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat
    17641 gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca
    17701 aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt
    17761 gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt
    17821 gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat
    17881 ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc
    17941 gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa
    18001 gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac
    18061 tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa
    18121 tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt
    18181 gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa
    18241 aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc
    18301 taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct
    18361 tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc
    18421 gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct
    18481 cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc
    18541 agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac
    18601 ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt
    18661 tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta
    18721 cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac
    18781 gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc
    18841 acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga
    18901 ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc
    18961 tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat
    19021 ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta
    19081 gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca
    19141 aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg
    19201 aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct
    19261 gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca
    19321 cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa
    19381 aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact tccaccataa
    19441 ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat
    19501 cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg
    19561 actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc
    19621 ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata
    19681 gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt
    19741 tttcatgagt tattcattaa attggaatct agattctatt ttttccggag gcagtcattc
    19801 tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg
    19861 cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt
    19921 gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc
    19981 tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc
    20041 tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga
    20101 ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat
    20161 tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc
    20221 tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat
    20281 tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat
    20341 gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg
    20401 gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc
    20461 tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt
    20521 gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt
    20581 taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca
    20641 agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc
    20701 agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc
    20761 tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta
    20821 ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt
    20881 taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg
    20941 ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt
    21001 cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat
    21061 caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg
    21121 attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa
    21181 gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta
    21241 ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa
    21301 cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa
    21361 gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac
    21421 ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc
    21481 aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat
    21541 ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg
    21601 attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac tgaaccaact
    21661 tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa agcaatcgct
    21721 acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt tgcttgagaa
    21781 actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat gtcacgacca
    21841 gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact tggatcttta
    21901 ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat tgctgcacca
    21961 ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc aggtgttggt
    22021 gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac actttgatct
    22081 gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga ttcaggcact
    22141 gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc tgttgctgaa
    22201 gattcaggca ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgttgattct
    22261 tctgttgctg aagattcagg cgctgttgat tcttctgttg aagattcagg tgttgttgat
    22321 tcttctgttg ctgaagattg tgtagctgat gatgattctt cagtcattga agattgtgta
    22381 gctgatgatt cttctgttgc tgagctttgt gcagtagatg aagcttgagt agcttgtgct
    22441 tcagcctctg cttgttcgcg tgcttctttt tcagcttttt cttgtgcagc ttgttgacga
    22501 gcttgttctg ctagacgttg ttgttcacga atacgagctt gctctgcttc agcttctgct
    22561 ttttgacggt tcaagtcagc tttcttgtct tcagcagttg cttgttcagc agctaatgaa
    22621 gttttcagta cgttcaaatc agcttgttta gataaaagat cgcctttttg agattccaaa
    22681 gcagcttggt tttcagctaa ttcttttagt ttagcatcgt tttcagcttt tttgtcttct
    22741 acagcttttt tatcttgttt ttgttgttcc atcaagtcgt tgtttgcttt taccatcgtt
    22801 gtcattgctt gaacgcgtcc aatagcatct gctaatgaat cagcattcaa tacagcatcg
    22861 atgtagtttg agcttgtgtt gcttacttgt gcttcgcgtg cttgtttttg gattgtatct
    22921 tcacgttttt caatacgttc ttgtaaatct gcaatgtctt ttactaattg tgcagattct
    22981 tgacgcaatg tatcttgttt tgctaatagg tcttgtgctt gtgtgttgat ttcagaaact
    23041 tggctttcta aggcatcgat ttgtgattgt gcatctgctt gttgattttt caagtctgca
    23101 atcttttgat cttgctgttg tatctgagaa tcaaaatcgt ccgccgcggc agcgatcggt
    23161 gatgctacgg cagtcaaggt cattgaacat accattactg ctgatattaa actctttttc
    23221 actcttcatt cctccgactg gcttaattta ataaatatct aaatatattt aagtctttct
    23281 taacgatata aaaattgtac catagcagtg tgtcaacgat ataatagtta tgttacaaaa
    23341 atgtttcaaa acaactttta aaaatgacaa aactgttcgt ttcagaaaat gatcatgaaa
    23401 aagccgcgtt aagtctagca tttcttaaga taacaacgat tctttttcgc tttcgaccta
    23461 aaaataaaaa aagaaattat tcatcactga ataatttctt aaagggaaaa atgaataata
    23521 caaatataat catgttcaac agcagtgtag gtcctaaaaa ccttgtaata aaatacgtgt
    23581 tatttactac tgctaattta aaaatcaact ggaccaccat cgtaaacaat tcataacccg
    23641 tgacaaagat gatcatactg aaaaattcag taaagatgtt gacatggata acgtctttca
    23701 ttacatacat caaccataca attaaaggga gagctactgc atagataccg ataacgccaa
    23761 tatagtaagc gtcatagata gccccgagaa caatcgtggt aatcagtaaa taacgttttt
    23821 caaacgccat actgcaacat aataatgcca atatcagaaa gtgggcattt gacatatagg
    23881 tgcctttcga ccactcccct agcattcttg ttaaatgacc atctatcaac atcaaaaaga
    23941 acaaaacgat tggcagatag tacttcattg tttctttctt caacatggac taatcctccg
    24001 ctagccgttg gacaatcgtc acaactgaca cgtcatacat ttctgcataa ggtttcacat
    24061 aaacttcccg atccagccca taactatctg gtttcgtttt aataaccgta ccgatcggca
    24121 agttagctgg agaattccct ccaagaccgg atgtctggac aacatcccct tctttgatat
    24181 ccgtatcacc agttaattgg gtcaccacta aggcatggag cttttcatca tagtttttaa
    24241 gcaaaccaaa cgcttcgcca ttagccgaag atacccgtac tggaaaatga ttggagcttt
    24301 cattagagga tgacagtaat tcgattttag acgaagccgt attgacctcg atcactcggc
    24361 caatcaatcc tttttgcgac atgactgcca tatttgcttc gataccgtct ttagaaccct
    24421 tgtctacgat cagcatgtcc tgccaactgt caggcgaacg agtaatgaca tttgccgtta
    24481 ctttttcgta actagtcagc gtctgattca actcaagttc ttttttcaac gcttcgattt
    24541 ctctctcgta attcttgttt tgctgtgaaa cgtcatcata gctatcgatt ttttctttta
    24601 gccgctcatt ttcggcataa gttgaaaaaa gattatggac agaatctact ccatttcgta
    24661 cccaccgtac tggggcagag attgttttat caaccattgc aacactgtca ttaacgatgg
    24721 attggaagaa attgggcttt ccttcattcg ctcgctgcgc agcggtcaca cttagtatcg
    24781 tcaccacgat gataacaata accaaggtaa taataatatt tttattagga ttgaacttct
    24841 tcacaaagac acctcgaatt aattagactc ttgtgttcaa ttttatcact aaatgccgtg
    24901 aaacaaaagg taagttgctc ctcttaagct tttttttagc aaataataga ttctgtcatt
    24961 tttagcctga aagacaaatt tttttatttg tttatggatc aatctttgct ttgaataact
    25021 gcaatcatac catctgaaaa agaaaattct gcggtatctg aaagaaattc gttacttgca
    25081 taagatgtcg gatattctac ttctttattt tccaatacat acttgctgcc ttttaatgta
    25141 agattcgaga cgggagtcaa gcagcaataa gctaaatact tcatatctgg tatctgtctt
    25201 actatatgct ctccaggcaa ataataagac aaataatttt gacaatctct gataataagc
    25261 tgatgggcaa agggtttgaa gcgaggttcc atacctaacc agagattggc taaaagatga
    25321 tccaatcttc cgcccgtagc tccaatcaaa gtgacttccg cttgcgggta acgttcaaat
    25381 gtttttaaga tacctaactg tgtatctgta tcgtcttttt ctgcagggga acgggtatac
    25441 tctttggctt ttgcaaatac ttccttctgc tcttcgttcg acaatgaatc aaaatcaccc
    25501 actgccagat ctactgggta gcctgcttga agcaggtaca gcgcaccacg gtctatcccg
    25561 acataggcat cgtatttgga cagcagagac tgcggccagt tttgcggatt acctccagca
    25621 gctaataaaa ttttcattta gtctaatgcc tcttttaatc gttcgatttg tccgactgga
    25681 ttttcggctc catagatata tgatcctgca acgaagacat ccgcccctgc atctttacag
    25741 atttttgctg tttctggaac gattccgcca tctacttcga ttgcataagt ccaattgttc
    25801 tctctacgta gatcagcgag ttccgtaatt ttttctactg tttcttcaat aaagctttgt
    25861 ccgccgaacc caggattgac agtcatgact agtacttgat cagccaaagg caaaacatgt
    25921 ttgatcatgg agactggtgt tcctggattg ataactactc cagctttaac tcctaagctt
    25981 tttgtcattt gaagtgcccg atgaatatga ggagttgctt cgacgtggac agtaatgata
    26041 tctgctccag cttttgcaaa tgctggaatg tagttttctg gatcaacgat catcagatgg
    26101 acgtctaacg gtaatttagt aacaggacgg attgcctgaa caatatttgg ccctaatgtg
    26161 atatttggga caaatcggcc atccattaca tctacgtgga tgtaatctgc gcctcctttt
    26221 tccactaact caatgtctct ttgtaaattg gcaaaatctg cactcaaaat ggatggtgct
    26281 atcttcattc ttgtttcctc ctattttcgt ttaccttctt tttttgcata cattggtttt
    26341 cttttttcga tttcttgtaa gaattgcaaa taattgtcgt aacgtgtctg tgcgatacca
    26401 cctgtttcta ctctatgttt tacttcgcag ccaggttctt tagcatgcat gcactctcta
    26461 aatttgcaat ggacagaagc ttctacaaat tcaggaaatt gtctcggcag ttctcctgct
    26521 tcaattgtca aaaagtcaat cgagctaaat cctggtgtat ctgctactaa cccgccatag
    26581 ataggaatca gttccacatg acgtgttgta tgccgtcctc ttccaagtgc atcagaaatc
    26641 tctcctgttg ctaaattcag ttccggtgat atttgattca gtagcgtcga ttttcctgct
    26701 ccagactgac ccataaagac cgatagccgt tctgggaagt aacggatcag ttctgtcgta
    26761 tcacccgtat gattgggtac aatcaccgga tagcctattt tagtataggt ttgcttgatc
    26821 tcttcgattt tttctgggtc gtctgacaag tcggtctttg tcaaaaaaat aatcggttcg
    26881 atcgactcgt attccaaggt aactagaaag cggtcaagta gattataaga aaatgcaggc
    26941 tctacgatac ttgtgacaat gattccaaga tcaacatttg tgactggtgg gcgaactaat
    27001 tgattttttc tcggcaagat ttccagaaga tagccgtctg tttgattttc actctcaaag
    27061 atcacctgat ctccgacaag cggcgtgatt ttccgattcc ggaaatttcc tcttcctctt
    27121 gtttgatagg tctctccgtt tgcgtataca tagtaaaaac cgctgattgc ttttctgatt
    27181 tgtcctttga taagtgccac ctccgttttt gtttatttta ccatacgaag aggaaagtag
    27241 gtgttattcc atgcaaactt ctcaaagtat atggcttgaa aattgatcaa aagagataga
    27301 aacacaaaaa agagaatgca acaaatcaat gtcgcattcc cttctgatat attttattca
    27361 gcacttattt tgaactttct gaggtgctag ctgatgaact tgtggagctg gaagattctt
    27421 cttttgaaga gttgcttgtg ctactggagc tacttgagct gctagaactg tctgaagagt
    27481 ctttttcaga actagaagac gctggatctg gtcctttaga gtaaattacg ttcacaacca
    27541 ttcctttggt aattgtcgtt cctgcccctg gtactgtacg aatcacttta tctttatcca
    27601 ccgtgtttga atattcggaa gtctcatgcc cgatatattc tgcaccgaca ctagctaaat
    27661 aactttgtgc ttcgcttttt gtatagccgg agatatcatt caatttaggc tctgttcctt
    27721 ggcttacgac aaaattgatg taagtttctt ctgctacaac ttcacccgag gctggatcct
    27781 gactgatgat cgaacctgat ggtacagtgt cgctatagtc ataagtagca ctaatattat
    27841 tgatattgaa tccttgtgta acaagccaat tacgaacagt ttcgtagttt tctcctacat
    27901 gactactcaa agtcactgtt tctggtcctt tgctgactgt gaaagtaatc gtgtctgttt
    27961 taggatcgac ttctgtatct tctgatggag attgcgaaat gatcattccg ctttctacct
    28021 ctgaactgaa ttcctcttct ttttgaatca agtcagcaga aaaccctaat tctttcaaac
    28081 ttgcttcggc atcttcataa cttttaccac tgtaatcatc taatttgatt tttttcgtgc
    28141 cgctactaat atacagagtg acttcgcgtt tttgcttgac tgtcgttccc gctgccgggt
    28201 ctgtcttcac tacattacct tcttcgattt tgtcatctgc aatttcttct gtttttgttg
    28261 ctacttctaa tttcgctttt gcgagtgttt ctctagcaga agcttcagaa agatcagaaa
    28321 catcaggaac aacaacttct cctcgcccgc cagaagcaaa gaatacagct gttccgattc
    28381 ctaaaacagc aagaatagct aataggatca gccaccattt tttctgtcct ttctttttag
    28441 gatcaggcag aacttcttcc tcctctggta aggtttcgtt ttctggcttg tcttttggca
    28501 gaggcataga tttgaatgaa tcaggcattg gtgcatcttc cgtaaggggt gtgataactt
    28561 ttgtttcatt atccatcaca tgaggctgcc atttttcttc gtttgcacga gctggtgata
    28621 atactgtaga aagatcatca gacatttcct cggctgactt ataacggtct gcaggttctt
    28681 tagccgttgc ccgtaaaatc acgttttcca gtgcttgtgg cacatttgga tcaagtgctt
    28741 ttatagatgg aagatcatct tgaaaatgtt ttaaagcaat tgttacagca gattcaccat
    28801 caaacggtac gctacccgtc aacatctcat aaaggatgat ccctaaagca taaatatctg
    28861 actgcttcgt tgccatactt cctctggcct gctcagggga cagataatga acagatccta
    28921 gcatcgtatt cgtctgcgtg atcgatgttt cagataaagc gatagcaatc ccgaaatccg
    28981 tgatttttac aacaccttca ttatcgatca gtacgttttg gggtttcaaa tctcggtgga
    29041 tgatttgatg gttatgagct aaagaaatag cagaaaggat ctgctgcata atatttactg
    29101 ctgtttcata aggaacagga taatgtgttt ggatatatcg tttcaagtcc attcctttga
    29161 cgtattccat caccagatac tgcatgttgt cttcttcacc aacatcgtaa acactaacaa
    29221 tgtttggatg aacgagttct gttgctgcta aagcttctct ctgaaatcgt cgaatcgcag
    29281 tctggtcatt ttgaaagtca aatcgtaaga ctttaactgc tacatcacga tccaagatta
    29341 aatcgtgcgc caaaaataca tttgccatac caccgctacc gatatttcca gtgatttgat
    29401 aacggccatt taattttttg cccaactcga tcatacactg tcctccttat attcgataag
    29461 aagaacggtg atattatccg ctccgcctgc ttcattggca cgatcaatca gctcagttac
    29521 tttatgagac aatgggcctt cttgattgat gatcgttcct atttcttctt cagacagcat
    29581 gttcgtcaag ccatctgaac ataacagcag tcgatctttt agttgccaaa gatgagtaga
    29641 aacatctact tcgactgttc caggcatgcc gacagaacgt gttaaaatat ttttccttgg
    29701 atggttcact gccatttctt cgctgatttc tcctgatttg actagttcat tgactaaaga
    29761 atgatcctcc gtcaattgca tgattttatc ttcacgaatc aaataagctc tgctatcccc
    29821 aacattggca atcgtgaacc agtcatctga cagagcggcc gctacgattg ttgttcccat
    29881 accgttatat tccggctgtt cttgtccacg ctgataaatc agcatatttt cttcttgaat
    29941 cgattgaatg aaccattggg cgattttttc atcgtcaaca gcttcttgtt tttcccaagc
    30001 ttcgcctaga ttcgttaccg ccatctgact ggcaatgtct ccggcacgat gaccacccat
    30061 cccgtctgct aatacagcaa gcttgagacc tgaacggctg gtaaatacac tggcatagtc
    30121 ttgatttgtg ttacgtcgtc ttcctacatc tgattgatat tcaatctgca ttttttcacc
    30181 tcattacttt ttgcgcaaac aacaaatgaa aaatccatct gtgtaaaact gatgcggata
    30241 caaagtcaac attttctctt cgattgatga ttgtagcata ggattggtca aaacatcaat
    30301 cctctcaaaa tcagggtgtc tttccaagaa agcagaaaca acgtcttggt tttcttcttt
    30361 taagatcgta caagtgctat aagtcagaat accggaagac tttaaagttg gtgcgcagct
    30421 ttctaaaatt tccaattgga tttttggtaa attcgctaat tcatccggct gttttttata
    30481 acgaatatct ggttttcgcc gaagcagccc aagacctgaa caaggcgcgt ctactaaaat
    30541 ccgatcaaac tgctctgctt cgaactgttt actgacttct ctagcatcca tcttttgagc
    30601 gtagacagca gaagagacac ctagacgtgc tgcattatct tcaatgagtt tgactttgtg
    30661 ttcgtgtaca tctaaagcga ccacttctcc accgcatgac ggatctaaaa aagaagcaat
    30721 atgcgtcgtc ttacctccgg gtgctgcaca ggcatctaag acatgatgtt caggctcgat
    30781 ttgcatagac ggcgctacaa gcatcgaact ttcatcttgg atcgtcatga cgccatcgtg
    30841 gaacaatgag ctagaagcta aatgcccttt tttcgctact ataccgtatg gactgacttg
    30901 gcttttttca gctgtgatct cttcttcttg caaaacagtg atggcctgat ctctggaaag
    30961 tcggcgtgta tctactcggg cactagcatg gctaggctga tatagcgata aaccgagctt
    31021 ttcagtttct tcgtatccga tttgatccac cagtcgtttc gtcatccata gaggcaggct
    31081 gattttgaca gaaagtcttt cgataggatc agcaatgcct tgcagatctg gtgctccttg
    31141 acgctgataa ttcctcaaga caccatttac aaatttccct gtcccaggat ttccttttac
    31201 ttttgcgatt tcgaccgcct cgtttaaaat agcatgtgca gggactttat ctaaaaattc
    31261 taattggtat acagaaagat aaagaagcat cttgacccag tcatccactt tttttgcttt
    31321 agcgatcaat ggtgcaacgt aaaattccaa tagtcgctga tggctaatcg taccatagat
    31381 caattctgta aataaaccca catctttttg atttagctcg ctttgtttga tcatctcatt
    31441 caacaaaaga ttcgagtagg cgccaccctt gttcactcgc tctaatgtca gtaatgccat
    31501 atatcgtact gattttttca tttttttagg gatttttttc attatttcac ctgctgacca
    31561 atctgaactt gctgaccgct tccgttaagg aaatcatgta cggcctgttt tcctttacca
    31621 gctggctgta attcattgat ggctaagacg gtctgtttac cgcaagcaat ccatagattt
    31681 tttttgtctt tccggatgat cgtaccaggt gctttgtctg tagtttctgc caatacttca
    31741 accgataata atttccagcg tatgccctca taggtcgtga atgcaattgg ccaaggacgc
    31801 attccgcgta cttggttgtc aatttcttct gcagttttat gccaatcgat tgcttcttgt
    31861 tcccgtgtga tgtttggcga aaaagttgct tctgactcgt cttgaggtac tggtttttgt
    31921 ccatccaaaa tattgggcaa tgtttctagc aatagctttc ttcctaaaag gctaagcttt
    31981 tcaaacatcg tccctacatc atcttgttta gtgattggta tgctttcttg cgcatagatt
    32041 ccgcctgcat ccattttttt gatcatttcc atgatcgtta caccagtttc tttctcccca
    32101 ttcatgatgg cataatgaac aggcgcacct ccccgatatt taggaagtaa agaagcatgg
    32161 acattgattg cccctaattt tggtacttgc aataactttt caggcaagaa ttggccaaat
    32221 gccgcagtga cgatcacatc aggcaccaat tcaatgattt cttccatttc tggcgaaccg
    32281 gagattttct ctggctgcag cacacggata ccgtgcttca gagccgcttt ttttaccggt
    32341 gtcggcgtaa ttactcgttt ccgcccaact ggacggtcgg gctgtgttac cactgcctgg
    32401 atctcgtaac ctgattctat cagcccttca agaatcggca cagaaaaagc aggtgtgccc
    32461 ataaatacta gcttagtcat caagatgttc ctccatatat tcttcaagtt cttcttccgg
    32521 gatctgctgt atcattttct caatgaacaa gataccgtct aagtgatcga tttcatgctg
    32581 gaaggcacgg gctaaatagc caaaagccgt cacttctatc tcttctccgt ctcgatcata
    32641 ataacgcacg gtcacttcat ctgctcgctt gaccgttcca tagacatgcg gaatactgag
    32701 acaaccttct acgtcaaggc tttcaccttt tgcttcgatg atttctgggt taatcagttc
    32761 aaatttctcg ccttcatcta cttcgatcac agcaattctc ttgttttgtc ctacttgagg
    32821 agcagcgatc cctattccgt cattcgcaat cattgtctca tataaattgt ctaataaagc
    32881 gattgtctcg tccgtgatta catcgattgg ctgagcagtt ctttttaatt tatcattagg
    32941 gtggatcaat ataggatagc gcataatttg gccctttctt aaataaagtt taatggttcg
    33001 ttgtcaattg ccacaaaaag accttgtcgc tgttctttct ggcttccatt caaaatttca
    33061 tccaaaacat gaggaagatt cggttcatgt ttatatttga tgattagttg ataataatat
    33121 cggtttttca ctctcaaaat catgctgggc gttggcccta atagcaagct ttgagggctt
    33181 aacacttctt ttagctgatt ggcaatctga aagattttct ttgccgctac ttgttcttca
    33241 ggatggctgg cggttatttt cacagtaaag taataaggag gatagcctcc acgatgccgt
    33301 acatgcattt cctgctgata aaactgctca taattttgtt ttttagccaa ttctatggcg
    33361 taatgttgag gattgaatgt ttggatcacg acttcgcctg ctttttccgc tcgccctgct
    33421 cgtcctgaaa cttgggtaag caattggaat gtatgctcac tagaacgaaa atccggtaaa
    33481 ttcagggagg tatcagcatt caacacccca actaatgtca cttctggaaa gtccaaacct
    33541 ttagcaatca tttgtgtccc taataatata tctgcttctt ttgcaccgaa tttttgtaaa
    33601 atctgttcgt gtgccccttt tcttcgagtg gtatctacat ccatacgcaa aatcctagct
    33661 tgcggataaa gttctcggag ttcctcttct actttttgag tacctgttcc atagtagcgg
    33721 attttattgc cgccacaatt aggacagcga ttgggaatag gttcttcatg tccgcaataa
    33781 tggcagcgca ttgaacgtgt atccatatgc agtgtaagag agatatcgca attaggacat
    33841 ggaagaacaa atccacagtc cctacacatc acaaaagacg aatatcctcg acgattgagc
    33901 agtaggacag tttgttcttt tttgtctaat cgattttgta ttttttcctg caaattagca
    33961 gaaaaagtag aggtccgatg attctgaaac tcttcacgca tatctacgat ctcaatagct
    34021 ggaagctgtg cagctgcttt ggctcggtgg tttaactgca ataactgata aacgcctttt
    34081 tgagctcgtg ctcttgattc taaagagggc gtcgcgcttc ctagaactat tgggcaatga
    34141 tgatatttac tgcgccaaat tgctagatct ctcgcatgat aacgcggtgt ctcatcttgt
    34201 ttgtaacttg attcatgttc ttcatcgata atgatcagcc cgatattttt cagcggggca
    34261 aaaatagcag atcgtgcgcc tactactact tgcgcttctt cccgctcaat cttgcgccat
    34321 tcatcatatt tttcaccttg ggaaagtccg ctatgtaaga ccgcaactga ttctccgaag
    34381 cgtcctttga atcgctcgac catttgcgga gtcaatgcga tttcaggcac taacataata
    34441 gccgttttat tttctgacaa aacatcagcg attgcctgaa gatatacttc tgttttcccg
    34501 ctgccagtga ttccctccag caaaaagacc tgatcttttt gctgctgacc tgctgtgatg
    34561 atttgttcga cagcattttt ttgttctgca ttcagttcca atgccgtcgt ttggtcaaat
    34621 actcggtctt tataagggtc gcgataacgt tctgattcta taaactccag ccagcccttt
    34681 tcttttcctt gattcaagac agcagtactg attcctttat ttttgaaata agcaattggt
    34741 tcttcttggc tcagttcaga taaacacaaa agaagttctt tttgtttctt ggcattttgc
    34801 cgtaattttg cccattcttc ttcgatttgc ttttcagtta acaaagaacg gaccaaacga
    34861 atcgttttta ctttatttcg tgtatggact tcatatttga tatctactaa ttgctcttgg
    34921 cgccagcgaa tcaattgaga aaggctgcct tcttcttctg cttccttcca ggaaatcact
    34981 tctcgctgat caaacaatac ctgaacttct ggtgtgtctg ccaaaggata gatcaattta
    35041 tcatattctg ctttcatcac actcggaagc atcgtttgta gacaagtgat tttgaatgcg
    35101 taagtaattt ttttcatata atcagctaat gaaagtaatt cttcattgac caccggagca
    35161 aggtctagca aacgaataac tgccttcaaa gatggattag ctgattcttc tgatttttgg
    35221 atagccataa caaatccttg gacatgacga ttgccattac caaaagggac ttctactcgc
    35281 atcccgactt gcaaagctgt ttccatttca gagggaacca aataagtgaa tggctgatcg
    35341 gtctgcatag taggtacgtc cacaatgact tcagctgtta atgtcatggt tcgtttcctc
    35401 ctttttttgt cttttcttat caagcgacaa aaagttcctt gcctatttta ctagagatcg
    35461 atggatttgt cttatcttct gccaaatcct aacaaaaaat taacagcaga taagaaaaaa
    35521 aagaactgtg tcaaaaagtt cagacacagc cttttctacc agacaataag tggcgaatgt
    35581 ttgaaaagga aacagctggg tttgcttttc tttttagatg ttcgctctat ttttctttat
    35641 tacatttttt gctcttcacg gatacgtgat tccaattcgt gttgttcacg ctctttttgt
    35701 gcttgacgtt cctcttcttc catcttcaaa cgttcacgtt ttaattcagg atgtggatca
    35761 ttgactacat ttcctgcatc gatttcttct aaagcttgac cgacgctttt tactgaatca
    35821 aaagaatcta gagtaggctg tgcacttgca tccaattcat gcgcgcgttt acttgctaaa
    35881 atcactagag aatattttga atttacacga tctaatagtg agtcaattga aggttttaac
    35941 atcataagac tacatctcct ttaacatttt aatatattta ccgattacgc gatctactcg
    36001 aaaatgttca ctggcaataa tgtttttgat tcgttcaact gctagcggca cttcatcatt
    36061 tacaacagca tagtcgtaaa gagccatcat ttcgatttct tcttttgcaa ctttcattcg
    36121 tttttcgatt acttcatctg catctgttcc tcggcctacg atccgtgatt ttaattccgc
    36181 taaatctggc ggagtcaaaa agatgaatac accatcgggc actttttctt tgacttgttt
    36241 tgctccttgg acttcaatct ccaagaaaac atcttttcct tcatccaatg tcttgttgac
    36301 atatgacaaa ggagttccat aataattccc gacatattct gcgtattcca gcatctctcc
    36361 agcttcgatc attgattcaa actcttcttt gcttcggaag aaataatcaa cgccttcgac
    36421 ctccccttca cgcattttac gggtagtcat tgagatcgaa tactgaaaat cattatcctc
    36481 actctcaaaa attgcttttc gtactgtgcc ttttcctaca ccggatggac cagacaacac
    36541 gattaataat ccacgctctg acatgacgac gtcctttcaa ctagtctttt tgtacttatc
    36601 gtttattttg cacttttttt ctgcaacttt caaggttatc tttaattata gctagaaatt
    36661 ggtaaaaaag ctaccctgta ccaagaggtc gtaaaaaagc tgtcctgcat caagtaataa
    36721 gaacagccaa acataaacag cttctcatgt ttttcgtttg gttggggtta ttatcgcaga
    36781 tgcaagcttt tgaacgccgt gtataagagg tcgtgaagaa gccgattagt ttcgagtatg
    36841 aagaaaaatt ttgtgaaaat agtttctcac atttttgcat aaatttggct taataccgag
    36901 aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac gaattttgtc
    36961 acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca attctgttta
    37021 tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca aagtattcac
    37081 tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt ctacctcttc
    37141 tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt cgttgatcaa
    37201 aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt caaagccgtt
    37261 tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt ggaacgttac
    37321 gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac ttgttctctg
    37381 attttttcta agatcgtctt agactggacg acttgatttt taatttcgat tgcatttgat
    37441 ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc taattcccga
    37501 cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat cgccagacga
    37561 tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt caacaaccgt
    37621 tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct tatttgaaat
    37681 tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt gaattcttca
    37741 gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact cgcctcaata
    37801 gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc catgctctct
    37861 tccggttttt cttctaccgt tacgaagttt tcgtccagca cgaagcgttc cgccatccga
    37921 ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt caattgagtg
    37981 ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt gccaatataa
    38041 gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat aagctgacga
    38101 aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc tagaaaacgc
    38161 tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg gatggctttg
    38221 ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat tttctattgc
    38281 tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca ttttttcatg
    38341 gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc tgactaggtt
    38401 cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt ctacgatttc
    38461 atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata taggatcagt
    38521 aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg ttttccttgt
    38581 agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg ctggtatttt
    38641 cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa atcatccggc
    38701 atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg atagatttca
    38761 ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc gcatgtctct
    38821 ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat gcttttgaaa
    38881 aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg ccagtatgtt
    38941 cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc taatcgtttt
    39001 ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc aaaaagcaga
    39061 aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc tggcatacga
    39121 atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt tgctcctttg
    39181 ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata tttatttttt
    39241 ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat ccaatctggt
    39301 atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc tggcagttta
    39361 gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa atccaaattg
    39421 tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag tgctgttttc
    39481 gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc agccatttct
    39541 ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc ttttttcata
    39601 tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt gataatctca
    39661 aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc accgtgcgct
    39721 cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa ttgtgtggtt
    39781 ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg agattgaaat
    39841 aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata ccgaaaaaat
    39901 tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac atctcctcta
    39961 cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa agcacattgc
    40021 ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa aaatggaagg
    40081 ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa gtaaactttt
    40141 tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata aaaaggtcat
    40201 gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac ctcctttaaa
    40261 taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca atgaaaacgt
    40321 gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa atgttgtaaa
    40381 ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag tttatgtaca
    40441 tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg actttgcaaa
    40501 cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt ctgctggaga
    40561 atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc agcgtttctt
    40621 ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact ttactgattt
    40681 aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt ttggtcatat
    40741 gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg tatacttcga
    40801 tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt ataggatttt
    40861 tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat cttttttcaa
    40921 aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc gtaatttgat
    40981 gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta aatatcgtat
    41041 cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt atcgagaaga
    41101 ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat tggtagaaga
    41161 tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa ataaaaagga
    41221 aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta tttacagctc
    41281 ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta ataaaacgat
    41341 gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag aaaagagcca
    41401 aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa taaaatgcca
    41461 aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc taatttgttt
    41521 cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg ctgcgcctgc
    41581 aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat aataaacttg
    41641 tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg cctttgtccc
    41701 tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa aactgctgcc
    41761 gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg ttccttctac
    41821 ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag tcacaaagga
    41881 ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac gctcgcataa
    41941 ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg gactcccctt
    42001 tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg tttgttttca
    42061 tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc gattttgtcg
    42121 attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc gatagctgaa
    42181 tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga agcatacaca
    42241 ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc tgctgtatcg
    42301 atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat attcattact
    42361 tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga tttcaaaaat
    42421 tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac gacaacatca
    42481 tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc catcaaatga
    42541 tcgactggaa tatcaaagaa accttggatt tggactgcat gaaggtccaa agtcagcatt
    42601 cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat tggttcccgt
    42661 gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac gttgattgtt
    42721 tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa gttatcattg
    42781 actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact ttcttcaatg
    42841 tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa ctcgacgccg
    42901 actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa aatttttaat
    42961 cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac aactctagaa
    43021 ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt gcaaaagtct
    43081 tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct tgctctcgca
    43141 attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc aatcgaactg
    43201 tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa gctgtggtcg
    43261 ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac accacatccg
    43321 acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc gactttagtt
    43381 cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac gttttcgcca
    43441 atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt cttgacatgg
    43501 ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact gtctacgatt
    43561 tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg gatttgtaca
    43621 cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta tgtcgttgct
    43681 gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt tctcatgatc
    43741 tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc aaaatcatcc
    43801 gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat atctgttaga
    43861 tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa caaagcttcg
    43921 ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc tggtgttgca
    43981 tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat tcgtccatat
    44041 cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt tccttggtga
    44101 tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc actgataact
    44161 aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc atgtcctgtt
    44221 cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc tttaacttgt
    44281 tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact gacccgatta
    44341 atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata taattttgac
    44401 ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc caactgcgtc
    44461 actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt ttcaacaaaa
    44521 caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga ccataacggg
    44581 cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt cacactactc
    44641 ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt aaacggtagg
    44701 cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta gctcaataaa
    44761 cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga caggacgctg
    44821 atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt aagccaaatt
    44881 tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga aactaagcgg
    44941 ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa tatttttcgt
    45001 ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg cgcgatgacc
    45061 gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga attcttcaat
    45121 caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca ctacaaggac
    45181 acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc tttcagaaga
    45241 tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt cccggcgtac
    45301 aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta cgccttttgt
    45361 ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa tgattcttcc
    45421 aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat atcctcctgg
    45481 aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt ttttcgcctc
    45541 ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga ttgtttccaa
    45601 ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt cactgataga
    45661 agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt catgcgggtg
    45721 attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt tcacctattc
    45781 caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc aaaaattcga
    45841 gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa gaagcctata
    45901 aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt tttcttctga
    45961 aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata aagactaatg
    46021 tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct gacagcatcc
    46081 ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg cctaatcgca
    46141 taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg gcaatcggga
    46201 tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc aaatgaacgg
    46261 gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc ggccgtttga
    46321 ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg acttgctccc
    46381 atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct gaattccctc
    46441 cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa atggcaatgg
    46501 agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca gctgctaata
    46561 caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct aacgctaccc
    46621 ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg atcactaaaa
    46681 acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac gctcttctca
    46741 tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc ggatctgtct
    46801 gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg atttcccatg
    46861 gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt catccatacc
    46921 tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac gcgctaaact
    46981 gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca tatcccacat
    47041 ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga gacgcttgaa
    47101 ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac taggaaaacc
    47161 tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt ttccttcttg
    47221 atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta acgatgcttt
    47281 caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt cacccgcatc
    47341 tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg tcaaatcaga
    47401 tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata ctttctttta
    47461 aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg atttgttcat
    47521 tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca ttcgacaatg
    47581 tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg ttgtttttct
    47641 tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct ggtgaaacgc
    47701 ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa gcagcatgct
    47761 gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt tgatccagtg
    47821 cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt ttatctggat
    47881 atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat accggatcga
    47941 gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat tcttcatgtt
    48001 cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc atcaaggtct
    48061 gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct gaactattat
    48121 acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt tcgaaatcat
    48181 gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct acgatctctt
    48241 ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct tttttgtctg
    48301 cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt accgtagctg
    48361 tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa ttttacgatt
    48421 tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat ccatcaattt
    48481 ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc tgctcatatc
    48541 tagccatttc actacaataa aaaactgaag aataccattt taggattcct cagtttttca
    48601 tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac ttcctcacaa
    48661 aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca caaggcaaaa
    48721 acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat catacgctct
    48781 ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact gtttcccatt
    48841 tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc gtggatgtct
    48901 tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga gacactcatt
    48961 cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg gatttcttcc
    49021 cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc tgtaccgact
    49081 tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa ttggttgatc
    49141 cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc tactggaata
    49201 ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt tagcaataaa
    49261 gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt tgtttctaca
    49321 atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac
    49381 atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc cagtcctaaa
    49441 tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg aaaagtctaa
    49501 gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc ctagtggttg
    49561 attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca aacaagttct
    49621 agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt tcctttttct
    49681 tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc tttcctataa
    49741 gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata agcagactgg
    49801 aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc ttgaaagaaa
    49861 gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc ttccgagtag
    49921 cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa ttcctcagat
    49981 tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat cggctgagcg
    50041 gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct agataataca
    50101 tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg aagccatttt
    50161 tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa
    50221 tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg agacaagtct
    50281 tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa acccaattca
    50341 tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata gcctaaacaa
    50401 tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt gccaactgga
    50461 tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac ttgatcttgg
    50521 tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg gcttgtagct
    50581 gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc actgccaagc
    50641 ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa tgagatgccc
    50701 aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa atgatttcct
    50761 gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta aacgataaac
    50821 aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac gaattttgtt
    50881 cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata taccaatatc
    50941 aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag taaagcaatc
    51001 gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc gagtaataaa
    51061 aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg caataatact
    51121 tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac catcatgatc
    51181 cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc
    51241 ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa caatagaagt
    51301 cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca aactactgct
    51361 acatcttgcc gaacagacaa ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc
    51421 attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa cataggatag
    51481 atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa taccgcaagg
    51541 gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt tggataaaac
    51601 tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa
    51661 ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg aataaaaaag
    51721 acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa tagtgcgact
    51781 tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa actagcccgc
    51841 agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt tgttacccac
    51901 gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc taagactctg
    51961 atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc cagaatcatg
    52021 agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg ccaactcatg
    52081 ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc gaaaaaaatc
    52141 agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg tttatttttc
    52201 acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta tcttatttat
    52261 tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag aaagatatcc
    52321 taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga atatggcaca
    52381 agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc
    52441 cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa
    52501 aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga aaccaaaaat
    52561 cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat tcatttctat
    52621 tgcttcagga aaatcattaa tagatttaga aacagcgctt gaagatcctc aggcctccat
    52681 cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa
    52741 aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg aagcaatcgg
    52801 cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg ctggcagctc
    52861 acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac tgaatggttt
    52921 gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt gtcaaaaatt
    52981 cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc ctggcggtac
    53041 aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa tcatcaaagg
    53101 aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat gatagcaatg
    53161 ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc ttcttttgta
    53221 ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt actttgatgt
    53281 gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact aaacgaacag
    53341 cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt ccagcttgaa
    53401 ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga cttcttaaac
    53461 gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct gagagcgcat
    53521 ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca tgatgagaag
    53581 cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc gcaccgatct
    53641 caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg agcaacccgg
    53701 cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca gctaattttg
    53761 caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat cgcagacgtc
    53821 cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac gctgcctctt
    53881 ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc atttcttcga
    53941 tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc cgtgcgattt
    54001 ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca tcgatgatta
    54061 aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt ccaatgatac
    54121 gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct gaaacttgat
    54181 ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga tctgcttctt
    54241 ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc agctcttctt
    54301 cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca attttttcta
    54361 gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct tcaattaatt
    54421 gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt ttttctaacg
    54481 agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct tgagatttta
    54541 attctaactt actttctctc agctcacttt caacttcaga acggtatttc tgattttctt
    54601 ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt gcactatcga
    54661 tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga
    54721 ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga atgttgaata
    54781 ccataaaatc cacctccata ctatcttttt catttttatc atctatacag cttctatgat
    54841 aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa aacatgcaca
    54901 actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag aaacggcttt
    54961 ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat ataggtaaac
    55021 ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga tatttttatt
    55081 ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata aaaaaacggc
    55141 aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata atttttattc
    55201 ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga cttcttcacc
    55261 aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag gatgattagc
    55321 catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat aagaatacca
    55381 agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt ctccttcttg
    55441 tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag gtgctacttt
    55501 gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt gtttcaattg
    55561 ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg
    55621 agtgatttct ggattcgcaa acatcacgcc tactttttca cggatttggt tgatgaaaat
    55681 agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt gagacatcaa
    55741 acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg ctcgtggaac
    55801 taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata ctaaagcatc
    55861 agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt
    55921 cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa tgaaagcggc
    55981 cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg ttttacctga
    56041 actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga cacctaaagc
    56101 gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga ttttttcgcc
    56161 caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg cagcatctaa
    56221 ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa atcagagcaa
    56281 catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa
    56341 tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc cttgcataac
    56401 cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg aaaccacgcc
    56461 gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta acgtgtccgg
    56521 tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac gtgcttgaat
    56581 ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata gctccggatc
    56641 aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc ctggaaatac
    56701 ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg tcaaactttc
    56761 cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt ttgctagcga
    56821 attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt tttcttctaa
    56881 tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt
    56941 cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct caatcagatc
    57001 ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa ctttagaaat
    57061 tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt ccacaaacat
    57121 tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat ctgtttgata
    57181 aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt cgataatctg
    57241 ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat atgccaataa
    57301 ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg cagttgcttc
    57361 ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga gttcacttct
    57421 tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta caactgtttg
    57481 ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg cattcgtatt
    57541 cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt tcattacttc
    57601 ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa agagggcaag
    57661 gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt tctcctgcca
    57721 aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt tgaaattatc
    57781 ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga tacttacatt
    57841 gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata gatagtaaag
    57901 atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct gaatgggata
    57961 ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac ttttcccggc
    58021 caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc tgtgacggct
    58081 aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg gtcaaccaac
    58141 atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa
    58201 ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt gatactagct
    58261 aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc tcctacgtta
    58321 aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat catacaaatt
    58381 ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt ccctattcta
    58441 cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa tttattcaaa
    58501 ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc gacacagatt
    58561 tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta atttatacga
    58621 tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt tttcccctgg
    58681 attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg caccaatcgt
    58741 gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat ttgctgccag
    58801 tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac gattgacagc
    58861 agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga tggcactgcc
    58921 agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg ttgttgattc
    58981 tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact cctcgactga
    59041 tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt gacgatcttg
    59101 ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta ataaaatgac
    59161 aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg cttttctaga
    59221 agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt tgcttttccc
    59281 atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt attgccgaat
    59341 aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc
    59401 caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata tttttttatt
    59461 caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt catcttctcc
    59521 agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg tttgggtgct
    59581 gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc ctgccaactc
    59641 ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa cttgttcctt
    59701 gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt caatcagctg
    59761 ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc gtatgtttct
    59821 ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct
    59881 tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt tttacttggc
    59941 tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac ggtgttccag
    60001 aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca attttcgtaa
    60061 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat tacttcgttt
    60121 gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt ttctcactaa
    60181 tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt cctactacaa
    60241 gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg gtctcatgta
    60301 aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta cgtctttcat
    60361 ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa ataaactttg
    60421 cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt gtccaagcat
    60481 tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag cgaatccaag
    60541 aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc
    60601 cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat cgataattcc
    60661 ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt ggaacaacag
    60721 attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg tgtctaatcc
    60781 tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac tttgtttgat
    60841 gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag ggaaattttt
    60901 tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct taccaactac
    60961 aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca agtcttgagc
    61021 tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg
    61081 atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt catacatttg
    61141 gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga agtaaggtgc
    61201 ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg tagaaaacag
    61261 atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt tcccaaataa
    61321 ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat gggcaattcc
    61381 atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat cgatcgaacc
    61441 ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca acaaatacac
    61501 ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct catattctgt
    61561 tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg atttgctcag
    61621 ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat tcttcatcag
    61681 ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag
    61741 agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac agttccaact
    61801 ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga agtacttgat
    61861 ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac gtatccacgc
    61921 tactagaggc ataataagct agactttctt tctctctgac attcatgaaa agtttagagt
    61981 gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt
    62041 aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc tcacgtactt
    62101 gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga taaactgttt
    62161 cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga tcaacatcgc
    62221 cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag gacgctagcg
    62281 aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc ttttgttccg
    62341 ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt ttatcttcct
    62401 tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa
    62461 actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct accgcttgag
    62521 aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct gcgtttagcc
    62581 aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa agctctgcaa
    62641 gttgcgcact taactttgtc tgatccgaat aattcaaact atttgtttcc aacatgctgc
    62701 tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa cggataaaaa
    62761 tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct tctgccaatt
    62821 gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc ttttaatggg
    62881 tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg caattcgccg
    62941 ttttttctaa atgatgttac actttatagt atagcataga tgttttttat caaaagaaac
    63001 agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac taaagacaaa
    63061 taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat gcgtggagat
    63121 gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa
    63181 gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat cacaggtact
    63241 tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg cgtcacattt
    63301 gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt tgtcttattc
    63361 ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa
    63421 gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct tcttgccaaa
    63481 caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga tacttctaaa
    63541 taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt tttttagatt
    63601 cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct tccaaataca
    63661 catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc
    63721 cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa
    63781 tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt ccaaaatgga
    63841 tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca gtatcttgaa
    63901 aaaacgtatg ccaagcttct ccatcaatgt catcaacttc ttgataagga cactgttgtt
    63961 ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct gttgacaatt
    64021 cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt ttattcttag
    64081 cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct cgtttgcttt
    64141 ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat ctgaatagct
    64201 gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga atactgaagg
    64261 ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg tctgagcaac
    64321 caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag ctaaagttgt
    64381 tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt ttcaagttta
    64441 ccataaaaaa aaaagaaatg caataaaaat aaataagaag ctcattaaga tttttctaaa
    64501 ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc tcccgtaagc
    64561 gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa aatcaacgcc
    64621 ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt cgcaattttc
    64681 gattgcttct tttaataaaa gatgctgaat tgctaaaggt tcttgcaatg tgatctctgc
    64741 taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat ctttttgatc
    64801 acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc ttataacgct
    64861 tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca atgcctgata
    64921 taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta aataataggt
    64981 gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa atgcttcttc
    65041 gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc tccataaatt
    65101 cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg atatgttatt
    65161 tattttttct cattaaatcg tccaattttt gggacaattt ttttaactgc tcttcattcc
    65221 atggagaatt acgtcggaaa tcaataaaat gcatatcttt attttcttgg gctattgttt
    65281 tttccgtttt ttttatcgtc ttatataatt cataagcaga agttcgtaat gctccttggg
    65341 aaaagatcat ccactgttct gctaaatcgc ttgtcgccac tgtcacttgt gtcaaacggt
    65401 cattcagctg accagcgatt ctctcgatat aactgtcagc cgtttcgtct tctttcgtaa
    65461 aaacaacatc caactggtat ttcttataat tttgttggat gcccggaact agttgggcat
    65521 cgaagacaac gatgacttcc attccttcat atttcgcata atttgataaa cgatgtaaaa
    65581 gagcttctct tgcatccgcc attttgtcct gctttttaag ctggacaagt tccggccacg
    65641 aaccaatcat gttgtagccg tctacaatca acagctgttt tttcatggtc tactccccct
    65701 gacgttggga aaaagcctga tacataagca aaccagcagc aacactggca tttaagctct
    65761 ggacgtgccc ggtcatcggg atcgtcagca attcatccat ttcttttttc aatcctggac
    65821 tcattccttt gccttcatta ccaatgacta aagcaattga tcctttggca ttccattttt
    65881 ggaagtttgt gccggtcata tctgtgccaa atacccaaaa tcctgtttct ttgagctgtt
    65941 gcacagcttg taccaaattc gtcacgcgtg caacagggac atgttcgact gccccagtgg
    66001 aagtctttgt tacgataggt gtgattccaa ctgctcggtg ttttggtatg atcacaccat
    66061 cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa attgtgtgga tcttcgatgc
    66121 tatccaaaat caaaaagaag gggtcttctt ttttctcttt tgttcgttca atcagttggt
    66181 caagggataa atattcataa ggtgtaatcg caagaacgac tccttgatgc acgccgtgat
    66241 cgctcaaagt ttctaatttt tgtttaggaa cccatttgac tggcacagct tgttcacgtg
    66301 cgatttcttt cagttcttct actttttctc ctcgtgaatc ttcttgaaga aagagtttat
    66361 tccctcgccc ttgttgtagc gcttctactg cggcatggtg cccaaagacg aaattatctt
    66421 ctaatgcttc ttcattttga tcagaaactg actgatttcg tccagtttgc tttttatttg
    66481 aatatctatt ttgttttttg tcttcaaatc cacgtttcga ttgatgtttg tttgttcttt
    66541 ctttacccat ttttttcacc aactttctga atacaccagc ggatcaattc ttccatccgc
    66601 tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt cgaaccctgt tgcgacccga
    66661 tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt tagcatttct tcctctgcga
    66721 taataaagtt cttcttcttc tgtcaaaaga tgttcttcca gcatttgctg aatcaaaaag
    66781 gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat gcagcttatt gggttttgtt
    66841 tctccttgat caatcaggtg gtcacgaaca tagatttcgt aaatcgcatc tccaacataa
    66901 gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc ttctcttctc catcgggttc
    66961 cttgcggtgt atcttctaat aagatccctt tttctttcaa caaatcacga atttcgtcgc
    67021 ttcggacaaa atcacgattg cttcttgctt gatttcgttc ttcgatcaat tgttcaatct
    67081 cttcatctaa taactcttct gctgtaaata aaataccgaa gatcgaaagc cattgcgaga
    67141 ataaagcttc tccttgtgcc aagacagcgg cagaaacttt cggctggtct agataagtgt
    67201 tcagccactt agcaagctca tagaccactg tgatcccatt tgctgcatta aaatcatcgt
    67261 ccatttcttc gatgaatcgc tgttctaaat tttctaacaa agcgatttta tccgcatcat
    67321 ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa attgtcaaac gtattcttca
    67381 gtcgttgtaa attggtggct gcttctttca tcgtcgtttc actgtagcgg atcggacgac
    67441 ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg atcaacgttt ttgatcattt
    67501 catgtacagt gatgaaattc cctagagatt tactcatttt ttcatcgtct tcgccaatcg
    67561 ttacatagcc attgtgcatc cagtaattag cgaatttttt ccctgttttc gcttcacttt
    67621 gtgcgatttc attttcatga tgaggaaatt ctaaatcttg cccgccacca tggatatcga
    67681 tcgtttctcc taaatgtttt gttgccataa cggaacattc aatatgccaa ccgggacgtc
    67741 ctttgcccca tggtgaatcc caagaaatct cgccaggttt tgcttgtttc cataaagcga
    67801 aatctaatgg gtcttctttt tgctgctgtt cttcccctgt acgctgactt gctccgactt
    67861 ccagttcatc gatggattgg tcacttaatt tcccataatt ttcaaattta cgggtacggt
    67921 aatacacatc acctgaagat tcataagcat agcctttttc aaccaacact tgaataaatg
    67981 ctaaaatatc tggcatatga tccatgactc ttggatgcag tgtagcaggt tcgacattca
    68041 acgctttcgt atcttcttca aatgctcgga tgaaacgatc agcaacttct ggtgctgtaa
    68101 tacctaattc gtttgctgta cggatgattt tatcgtctac atccgtgaaa ttcgatacga
    68161 aatcgacttt atagcctcga tattcaaaat agcgtcgaat tgtatcgaaa gcaatggtgc
    68221 tacgagcatt tccgatatgg atataattat acacagtcgg tccgcagaca tacataccga
    68281 catgtccctt ttgtataggg atgaattctt ctttttttct ggtcaatgta ttgtagattt
    68341 ttatcattct gatctcctca ctttttctcc tttgattcga acgacttttg ctggtactcc
    68401 taccgctgta gcgtcgtctg gaacatcttt caacacaacg gcacctgcac cgattttggc
    68461 gttttttccg atcgttaccg gtcctaatat ctgcgcattc gcagagagca tggctccttg
    68521 tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg ccgcctaatg ttacgccatg
    68581 gaataaaatc acatcgtcct cgatttctgc ggtttcgcca atgacgattc ccattccatg
    68641 gtcgatgaac acgccagttc caatcgttgc tcctggatgg atctcaacac cagttaggaa
    68701 acgccagaat tgagcattga tcttcgccaa taaaaagagc cggtgctgat ataaaaaatg
    68761 agagaaacga tgccaaaaca aggcatgaaa accagggtat gtcaaaacga tctcagctgt
    68821 cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc gctcgggtaa accaactcat
    68881 gactttccac tcctttcgta agaattttat gtaggaaatc atcttcttat aaagaaaaga
    68941 agcgcctttt aaataagttt ccttatcaaa agacgctttc cgcgtggttc cacttttctt
    69001 cataatcaat gattatctct gacagctaac gcctgttata cgttcttggc tactttgttt
    69061 cctataaaac agtttttcct gttttctgtg tttcgccaag accactccca gaggcatttc
    69121 ggcaaccatt cattgatacc ttgcaccacc cggtatctct cttgtaatga tcagctgctt
    69181 acttttttct gttcatcgtg tttagttcag tacttgattt aaatgtgcta atgatttttc
    69241 acgaccaagc aattcgatcg tttcaggcaa ttctggacca tgcatttgtc ctgatacagc
    69301 tacacggatt ggcatgaaaa gattttttcc ttttacgcca gtttcttttt gaacagcttt
    69361 gatggcagct ttgacagttg gagcatctac tacttccatt tgttctagct gtgctttgaa
    69421 tgcgtggaga acagtaggaa ctgtttcacc agcaagtact tcttttgctg cttcattcaa
    69481 tacaggatgt tcattgaaaa atagcgtaga cagatcaacg atttctgcag catagctcat
    69541 ttgaggttga tacaggctaa cgatcttctt cacccattcg attgtctttt catctgggtt
    69601 ttctgctaca cgtccatcag cgattagata aggcagacac atgtcagtaa atgcatctag
    69661 atccattgct ttgatgtaat ggttgtttac ccattgcagt tttttcgcat cgaaagctgc
    69721 gggtgattta cttaggcgtt ttggatcgaa cattttgatc aattcttggt gttcgaagat
    69781 ttcgtcttcg cccacaggtg accagcccaa taatgcaata aagttgaaca ttgcatctgg
    69841 tagatagcct aattcgcgat attgttcgat aaattgcaag attgattcgt cacgtttgct
    69901 tagtttttta cctgtttcac tgttgatgat caatgtcata tgtccgaagg ttggtggtgt
    69961 ccagccaaat gcttcataga tcatcagttg ttttggtgta ttggcaatat ggtcatctcc
    70021 acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc gcaacagcaa aattataagt
    70081 tggcatacca tcgcgttttt ggatgacaaa gtcgccaccg atattatcgg attcgaacga
    70141 aatagcgcct ttcaccatat ctgtaaatgc gtattctgta ttgcgaggca cacggaaacg
    70201 tacaacagat tctagacctt gtgcttcttt ttctgcttgt tcttcaggtg tcaagttcgc
    70261 acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt tgtgcttccc gttctgcttc
    70321 taattcttct tctgtgcaat agcatttgta tgctcgattg ctagcaagca gctgatcaat
    70381 cagcggttga tagatttctt tacgttcaga ttgacggtaa gggccgtatt ctccaggatt
    70441 ttcaggagat tcatcccaat tcattcccaa ccaagcaagg ttttccaact ggcttttttc
    70501 gccatcttcg atattacgtt tttggtcagt atcctcaata cgaataataa agtctccatc
    70561 attgtggcgg gcaaacaaat aattgaataa tgcagttctt gcatttccaa tatgcaggtg
    70621 cccagttgga cttggtgcat agcgtacgcg tactttcgtc attttcttcc tcttctttca
    70681 aactagactt tgactaacag caaaaattcc gtcagttacg gcttctcaaa tatcatttat
    70741 ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa atagtaaagc caactggttg
    70801 aatttcctta ccagttggct gaatttcttt tttattttga ttgtttagtc tttttctctg
    70861 tctctttttt attgtcgatt cctcgtcctg catgtgcagg tttagcaaag atcatgcgtc
    70921 cagcagctgt ttgaagggca cttgttacaa cgacttcgat acgttcattc atgtaatgct
    70981 gtccatcttc tacgacaacc atcgtgccgt catcaaggta ggctactcct tgttggcgtt
    71041 ctgtcccggc ttttactact agaacattca tcgtttcacc agggatgacg actggtttta
    71101 cggcatttgc caacgcattg atattaagaa caggtacatt ttggaattca gagactttat
    71161 tcaagttata gtcattggtt acaatgacac catctaaaag cttagccaat ttgatcaatt
    71221 tgctgtctac ttcactgata tcttcaaagt ctccatcgta catttcgaca gatataccct
    71281 cttctttttg caaggcattt aggatatcta atccgcgacg accacgcaca cgtttcaaac
    71341 tatcccctga gtccgcgatg tattgtagtt cgtaaagaac aaaattaggg atcatcaagg
    71401 ttccttctaa gaatccagtc tttgtaatat cataaatccg accgtcaatg atcacgcttg
    71461 tatccaagat tttatattta tggaaatttt ctcctgattt tcgttctaac agttgactct
    71521 caacttgact agctgtttct tgctcgattt ttttgcttcg tgaaccaaaa actttcttcc
    71581 attcttcgat tcgtgtcgtt cccattctca atcccagata gcctaagatg atcatcagta
    71641 aaatcggcaa tacactattg acgaatggaa tttccatatt gtaaagaggg gtcgagatca
    71701 caacacctaa agtcaatccg atgatcgcac cgatcgcacc gaagagaaga taagtcaagc
    71761 tgacctcact tagtttttgt tctacttgtt tgataatagc tgagatatgt ttggccaaca
    71821 gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct gttggtaaat gtattattca
    71881 gccatccgtt tgactgttga ccgattgcct gccaagctga gggtaaaaat gaaataccca
    71941 agcttgcgcc gacaatgatc atcagcaaag taatgacacg tttttgcatc gtttttcctc
    72001 cgtttcgaca atcatttatt agttagcgga acacacgctt caacgtttca gccaaagttg
    72061 ctactccaac tatctcgatt ccttttggcg cttcccagtt acctaagttg tttttaggta
    72121 ggtatacttt tgtgaagccg agtttttgga cttctttgac tcgttgttca atatgactga
    72181 cacgtctgat ttctccagtc agtccaattt caccgatgaa acattccgtt ggctgtgttc
    72241 ctttttcttt atagcttgaa gcaatactga cagcgatagc taaatcaatc gctggttcat
    72301 ttaattttac cccacctgca gcttttaagt aagcatcttg attctgaaga agcaacccag
    72361 ctcttttttc cagcactgcc atgatcagag atacccgatt gaaatcaaga cctgttgttg
    72421 ttcgtttggc atttccgaac atagtaggtg tgactaatgc ttggatctct accaaaattg
    72481 gacgtgtccc ttccattgcc acaacgatag cagagccagt tgcaccgtct aggcgttctt
    72541 ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc atgctcatgc atttcaaaaa
    72601 tcccaatctc attcgttgag ccaaatcggt tttttactgc tcgcaaaatc cgaaaactat
    72661 gatgttgttc accttcaaaa tataacaccg tatccaccat atgttctaac attcttgggc
    72721 cggcgatcga gccttctttg gtaacatgac cgacgataaa aatggctatc ccatttgttt
    72781 tagcggtttt gagtagttct gcagtggttt ctcttacttg gctgacactt cctgccacac
    72841 ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat gataacataa tcaggggaga
    72901 tatgttcgat ggcacgactg atttcgttca tatctgtttc agcgtataga taaaattctg
    72961 tgttgatact tccgaggcgt tccgcgcgta attttatctg ctctgcgctt tcttcgccgg
    73021 atacatataa gacttttccg cctatggctg ctaattgctg agaaacttgt aacagcaaag
    73081 tggatttccc gatacctgga tcgccaccaa tcaagaccat agaccctggc accacacctc
    73141 cgcctaacac acggttcagt tcttctaatt ttgttttgat ccgaggttct tttttaggga
    73201 cgacgtctgc gatttttgtt ggttttgctt tttctcctgt caaacttgtc cgagttctgc
    73261 ggtccgttgt atcttgttcg atttcttcga ccatcgtatt ccattttccg caatttgggc
    73321 agcgtcccaa gaacttaggc gacacatacc cgcaattttg gcaaacaaat tgtacttttg
    73381 atttttttgc cattgtaccg cctcctttta tcctaatcat gagacattaa atcaagctta
    73441 agtatcttta acgttttatt tactggaaga accaaatccg ccttgacgtt gagcagtcgt
    73501 ttgatcttga tctgctttta aaaacggcaa aaagatccct tggccgatac gctcgccttt
    73561 tttgatcact acatccttga aaccaaagtt ggtgaattgg aacatgatat ggccttcatt
    73621 tgcctcatta ttgtaataat cactgtcaat cacacctact ccgtttgtca acactaaaaa
    73681 ccgcttcaac ggattgcttg aacggttagc aagctgcaaa aattcatctt ctcccatgta
    73741 agctttgatt ccagtaggca cgagggttgg tttcaattgt ttttcatttt ctgatacatc
    73801 ttttttcaat ccatctgcga ctgtttccaa cccctttttc cagcctgctt tccagatact
    73861 tggaatcacg acctcttctg ctgcttcaaa atcatagcca gcagcatgat gtgtagcgcg
    73921 ctgcggtaga ttgatatttt gatctttata tttagaaata acttcaaatc ctctttttct
    73981 caccggtttt cctccctaaa taaactactc ctattctaac atatccgagg ctgtaaaaga
    74041 ctttctttac aaattcttca agaaattctg ctatcttaaa gtaaaccatt aattcgttct
    74101 ttttccaaac gactgcaagt tgtacaaaaa aactattttt tacatgactt tactcgtcaa
    74161 aacaagtaga atagaacgaa attaatgagt ggatatttga ttgaggtgtc acatgcgtaa
    74221 agaaattaaa aatgcaaaac gaattattgt caaagtagga acaagtacat tgatttatcc
    74281 caatggaaat atcaatcttg cggcaatcga tcagttagct tttgtccttt ctgatcttag
    74341 aaatcgggga aaggaagtga tcttagtctc atcaggagct gtaggcgttg gtatgcataa
    74401 attacatttg tcagaacgtc cgactttgat tcctgaacag caagctgcgg cggctgttgg
    74461 tcaagccgaa ctgatgaata tctataatca aagattcagt gtatataatc aacagacggc
    74521 acagatcctc gtgacaagag acgtggtaga ttatcctgaa agtcgtaaaa atgtgaggaa
    74581 tacattggaa cagcttttga ttatggacac gatccctatc gtgaacgaaa acgatactgt
    74641 agcagtggat gaattagatc atctgacaaa atttggcgat aatgatcagc tgtcagccat
    74701 catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt tctgatatcg acgggtttta
    74761 ttcagataat ccgactacaa atcccgaggc tcagatgtat cgctatatcg ataaaatcga
    74821 tgaaaagatt ctggcacaag caggtggtgc tggtagcaca tttggaacag gcggtatgca
    74881 gagtaaatta aaagccgctt ctaggatatt tgaagcaaac agcgcaatga ttttagcaaa
    74941 tggaaaaaat ccggcaatta tttttgatat tcttgcagga aaagaagtag gtactttatt
    75001 taaggaggac ttgaaatgac tgacttgatg caattaggac aacaggcaaa agatagcgcg
    75061 caacaactag cacttatgcc tacaaaccgc aaaaatgagc tacttgttca aatggcacaa
    75121 accatcaaaa agaatcaaca agcgatcatt gaagcaaatc ataaagactt agaaaaagca
    75181 gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc agctgacacc tgagcgaatc
    75241 aatgccatgg ctacagagat cgaaaaaatt gcttcgttag acgatcctat tggaaaagta
    75301 gatgagatgt ggacgaacac cgacggattg agaatcggca aaaagcgcgt gccactaggc
    75361 gtgatcggca tcatttacga atctcgtcca aatgtcacga cagatgcagc tagtctagca
    75421 ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag aagcattctt ctcgaatcaa
    75481 ttattagtcc aattattgca gcaagtactg gtcagtgctg gcgaatctcc ttatgccatc
    75541 cagtttgtcg atgatacttc tcatgaaact gcaggacagt tgatgcgatt gacagaatac
    75601 ttagatgtgc tgattcctcg aggcggagca aaattaatcc aacgagtcaa agaacaggca
    75661 acggtaccta tcattgaaac aggtacagga aataatcacg tatatatcga taaagaagca
    75721 cagttatcta tggcagtgaa catcatcgtc aatgcaaaag cttcccgtcc atctgtctgt
    75781 aacgcggcag aaacattgct gatccattct gagatcgcac cttttttctt gccagctatt
    75841 gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg atgctcgtgc gttagaatat
    75901 ctagagacgg ctgctttagc agaagacaca gattgggata ctgaatatct agattatatc
    75961 cttgctgtca aagtggtcga ttctttggat gaagcaattg cacatatcaa tcggcataat
    76021 actaaacatt cagaaacgat cgtgacagat agttatgcgg ctagtcaacg tttcttaaat
    76081 gaagtggacg cggcggctgt ctatgtcaat gcctccactc gttttacaga cggatcggta
    76141 tttggttttg gtgcagagat tggtatctct actcaaaagt tgcatgcccg tggtccaatg
    76201 gggttaaatg aattaacttc cacaaaatat atcatttatg gcgatggaca aatccgcgaa
    76261 taatgtttcg tgtgaaacat tattcccgcc ttttgccttt tttctgtaaa ggttctatcc
    76321 aactagaaag gtgtcaatat gtttgaaatt ttaatgattt tttttattta ccttatttct
    76381 ctgaatatcg ctgcttttct gggagtcggt attttatccc ttttctttca attcaaaaaa
    76441 cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt ttgacaaaat cgggcaaaaa
    76501 gggctggtta cacgcttgca cattagttac atggttgcat tatgcttatt agcgacaatc
    76561 aattactatt cttttttcga tcattcaatc gcttatacga ttacgctact gattgctggg
    76621 atttttcact tatcttataa atatcagctg aataaaaacc acttgaatcg gactttccgc
    76681 taaaaaatat ccgtacgata agtagagtgc cttgaagatc ggcaaaaaac tgccggactt
    76741 tttagaggct gtgacaaaga ttttgtcaca gcctcttttt ccatataaac ggcgttcgcc
    76801 taccagctgc ctggtaaatc tgtttcctct tcgttaagaa gcgaaaatcc gtcaaatgtt
    76861 tcgccatcat aataacgaag cagctcatcc atggacaagt tcatcgtttt ttctttttct
    76921 tcttccgctg attcagccag tagctgttcc cgatacacct gatacatacc agctattttt
    76981 tgctgcatcg cttttctagt ttcaccttcc gcattgatta ttggcaattc tttgatatag
    77041 ccacgataaa tcaaccgatc atctgaaaat tcaatgggta attcaacgag tgtcaacccg
    77101 acttcctgaa aatattcttc cagcatctat tatcccccca ttctttttct taaatgaatt
    77161 ttagcttacc tactgaaaat tttccagttt cattattttg agaaaaaaag ggaatgtgac
    77221 aaaaatcgtg tcacatcccc tttttccgaa taaatggtgt tcttctgcca actcctcaat
    77281 cataagtcac aatattcaac aaacatgaga aactgttttt tgaaattgtt ccttgtgatt
    77341 cagagttaaa cgacagtctc acaacctctt ggtaaacggc gttcaatcag ctgaccctcg
    77401 gtattagctc aaaaaacggt aaaatatgag aagctatttt tccgttttgt ttctctaata
    77461 cttaggataa aacgctgatt ttacaacctc tgttaaacgg tgttcaatca gctgtccttc
    77521 ggtattagct caaaaaacgg caaaatatga ggaactattt ttccgtttgt ttctctaata
    77581 ctcaggacaa aacgctgatt ttacaacctc ctgataaacg gtgttcaaaa gcttgcatct
    77641 gcggtaataa gcccaaccaa acgaaaaata tgagaagcta tttttgtttg gctgttctta
    77701 tgacttgatg caggacagct ttttcacaac ctctgatttc ttattcttct tcatttgtag
    77761 caaaataatt acggtgataa atgatacaac atacactgat aagaacaatg gcacctgcaa
    77821 aataaaacgc atagctttca ccaatatgtt caccaatcac acctgcaata aatggtgcta
    77881 aagctccacc gaaaaagcgg acaaaactat agctggacga agcaacgttt cgttgcagac
    77941 ctggtatttc catagcaatc gtcgtcaaca gtgtattgat aattccttgg aaaaatcctg
    78001 ctaacacaat gctgatcggt acaaggactg gcatagaagt tccagctcct aataacagca
    78061 aacaaataat gaataagaca aatgctgcta aaattgtccg ataagtcgta aacttccgct
    78121 caagagccgg tgcaacgaaa atcgaagcaa tagccagaag cacaccccag ccaaaaaata
    78181 caaaaccgac ttctaattca gaatagccaa tcaataaaaa tggcgcatac gcaagtaaag
    78241 taaagaatcc gaaattatat aacaatgcga ttagtccaac tgaacgaagt ttatgattgc
    78301 ttaacgcttt gacaccttcc caaaggccta cttttctctt tggtttctca gtttcaggta
    78361 aaagtaccgc tagggctata aaaccgcaaa acatcaaaaa tgctacccca aaaaatggat
    78421 aacgccatga accactgccc aagaccccac cgactagagg gccaacagcc attccgcagc
    78481 caagtgctgc ttcatacatc atgattgctt tttctgtgtt accaactaaa atactgacga
    78541 tcgttgctaa agcagtagag acaaataaag cattcccgac accccagccg gcacgtaacc
    78601 caatcagcat tccgatattc ccagataatc cgccaagacc agcgaataca acgatgataa
    78661 acaatcctgt catcaatgtt tttttcaaac cgatccgact agataagaaa cctgtaaata
    78721 gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt tcagctggcg
    78781 tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc actcccataa
    78841 aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt gtttcttcat
    78901 ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc tctctattag
    78961 catcatacat gatttatttt acatgtcaat atgcacatgt aaaacgtgga aactttactg
    79021 tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt agcctatata
    79081 ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca gctgatcaca
    79141 attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt agaccagcag
    79201 ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta ccacttgact
    79261 gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc tcctacgcaa
    79321 aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac aacagctact
    79381 ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa tcaaaaaaaa
    79441 ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt gatccagcgc
    79501 agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga acaaatgcag
    79561 cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga agagcaaatt
    79621 atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg acaggccaaa
    79681 acctatcgtt cggatcacta tttttctaga actgcattca agagactatg acaaaaagca
    79741 tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa ttgcttttag
    79801 cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg caatcacatt
    79861 cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt gttccatgat
    79921 cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg aaatagttaa
    79981 gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt ttttcgccag
    80041 cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca ctagaccaat
    80101 caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc tcatccccaa
    80161 gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat ttgtaggcaa
    80221 tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa aaatttctcg
    80281 taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc gtacgctcga
    80341 caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc attagcttct
    80401 ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac agcaacacat
    80461 tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc ccacttaatg
    80521 tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc aaactacgga
    80581 caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat tcttcagatg
    80641 tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag agaaggattc
    80701 cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca ttttcaccga
    80761 caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt aatgttctcc
    80821 cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg agttcaagag
    80881 attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt tctcctattt
    80941 tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt acactgtctt
    81001 tttgtttaga ggaagacaac cgatccagtc tgatttgttg ctttttcttt ttactggaca
    81061 ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc aatcgttttt
    81121 tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat tccttttgag
    81181 tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt acttcccaga
    81241 ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc agcgtaccat
    81301 aataataacg aagctcttca atcagtcgat caatactttt tctatctaga tgggttgttg
    81361 gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac tcgatctttc
    81421 gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac tggaaatgac
    81481 tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga ggcaggtagt
    81541 tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg gctattagtt
    81601 tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga tcttgctgat
    81661 agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact tgtagatttg
    81721 ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta aaacaacaat
    81781 tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc acatcttttc
    81841 ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt tcacctattc
    81901 tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta tacatgtctt
    81961 ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat gcagtcattt
    82021 ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa tagctactga
    82081 atagtaactg aaaacctttt attcgaacat agtgatcttt tctggtgcta aacgaacaga
    82141 ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa cataacggtc
    82201 tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa ttggatttgt
    82261 ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc tagaatcgac
    82321 tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa tgatcgcagc
    82381 taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt ggttgtagat
    82441 ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg cagaagaagt
    82501 atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg cttcctcgct
    82561 ttcagcgacc acaaagcgcc atggtttcat attgacagaa gaaggtgcag tcgtcgcttc
    82621 tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat atacgcgaac
    82681 ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta ttattaacct
    82741 ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa agtgttagaa
    82801 attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta tctttatggt
    82861 ccatttcaca atgcttgatt ttcttctggg ctttttctgt tagcgctaca tacacttccc
    82921 ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat accttcagat
    82981 gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt tgtagcaatg
    83041 gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata ccgatatttt
    83101 cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa aggtgactaa
    83161 tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg attgacttgt
    83221 caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa aaaaaacgct
    83281 ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa aaatgataaa
    83341 gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa gatagctctt
    83401 ctgcagtaga taattttaca ccgccaaaaa tttccgaaat cgccttttgc aagaatcccg
    83461 tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta tgacaagatg
    83521 ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg taataagttc
    83581 aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac ttgatgcagg
    83641 gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca tatctccagc
    83701 ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg gtaccagttt
    83761 tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa aaacttagac
    83821 cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc attgtgtaat
    83881 gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg tttggtgctt
    83941 gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt tctccggcat
    84001 tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc ggagaagttg
    84061 aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg ttcaatgtag
    84121 aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca tctttgtaag
    84181 agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga aaagaagtcg
    84241 tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat tttctaaaag
    84301 cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc gtaacagatt
    84361 ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa gccaaagtac
    84421 tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca tataagtcat
    84481 tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct tgttgagaat
    84541 tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca gacaaatctg
    84601 cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt tcttctgcat
    84661 tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt tgttctgttg
    84721 tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct gactgcaaag
    84781 tactttctgt atcgttttca attcctgttt ctgaagaact cgatgtctga gttgcgacag
    84841 tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt cctaaaagaa
    84901 taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt atgaaacttt
    84961 tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat ttaaataaca
    85021 tttgtttctt tttaataaat ttgctttttc tgcataaaaa gacgtaaatg aagaatattc
    85081 ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa gtaattctct
    85141 ctggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa tcatgttgct
    85201 gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta tatcttcaga
    85261 tgttaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt tgtcgctgat
    85321 ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt tgatgttatt
    85381 ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt ttgttccgca
    85441 ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg aaatgatatg
    85501 cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta acacacccat
    85561 gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta atggagcggt
    85621 tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt ccatcgctaa
    85681 atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa tatatagctc
    85741 agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg cagctataac
    85801 tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc cgcttgaagc
    85861 gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct cctgttttga
    85921 aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa taatccgttc
    85981 gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg ggacatcttt
    86041 cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc ctggcagaat
    86101 agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta ctgacaagcc
    86161 tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac gaggaccaat
    86221 caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt gacgattgat
    86281 aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag gcagcagaac
    86341 agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc ggatatctgc
    86401 tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttccg cttggatctg
    86461 atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga tttgtttttc
    86521 gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga cggcttatga
    86581 tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag tggtatgctt
    86641 ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt tagcagtagc
    86701 tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac ctactctctc
    86761 aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc gaggctcccg
    86821 agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag aaatcttgtc
    86881 gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg gagaaatcta
    86941 cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc attcgttgat
    87001 caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg atatcataga
    87061 caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa acaaacaaaa
    87121 ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac atcggaatca
    87181 tcctttagca aagcaaacag tcatccatcc gcaggaactg aaaactgttc ctttgatcat
    87241 ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca gcatagatca
    87301 gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg tcgataacgg
    87361 gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg aaaaagtatt
    87421 catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa aagaccagcc
    87481 tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca gttaagtaga
    87541 gtttttttcc ttcgctttca gctttttaac aacgaataag ttaagcaaaa gatccaatga
    87601 ttaaataaca taagtttctt aatttgttcc ttccttttat ttc
//
LOCUS       DAFCHQ010000004        78504 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000004 DAFCHQ010000000
VERSION     DAFCHQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.004"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..642
                     /locus_tag="LNC71_000304"
     CDS             10..642
                     /locus_tag="LNC71_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465402.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(776..901)
                     /locus_tag="LNC71_000305"
     CDS             complement(776..901)
                     /locus_tag="LNC71_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6465403.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1035..1928)
                     /locus_tag="LNC71_000306"
     CDS             complement(1035..1928)
                     /locus_tag="LNC71_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBK6465404.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2110..3036)
                     /locus_tag="LNC71_000307"
     CDS             complement(2110..3036)
                     /locus_tag="LNC71_000307"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBK6465405.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3121..3882)
                     /gene="pflA"
                     /locus_tag="LNC71_000308"
     CDS             complement(3121..3882)
                     /gene="pflA"
                     /locus_tag="LNC71_000308"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBK6465406.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4126..6357)
                     /gene="pflB"
                     /locus_tag="LNC71_000309"
     CDS             complement(4126..6357)
                     /gene="pflB"
                     /locus_tag="LNC71_000309"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBK6465407.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6634..9084)
                     /gene="parC"
                     /locus_tag="LNC71_000310"
     CDS             complement(6634..9084)
                     /gene="parC"
                     /locus_tag="LNC71_000310"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBK6465408.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9096..11141)
                     /gene="parE"
                     /locus_tag="LNC71_000311"
     CDS             complement(9096..11141)
                     /gene="parE"
                     /locus_tag="LNC71_000311"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBK6465409.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11307..11741)
                     /locus_tag="LNC71_000312"
     CDS             complement(11307..11741)
                     /locus_tag="LNC71_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBK6465410.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11868..12506
                     /gene="plsY"
                     /locus_tag="LNC71_000313"
     CDS             11868..12506
                     /gene="plsY"
                     /locus_tag="LNC71_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBK6465411.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12638..13513)
                     /locus_tag="LNC71_000314"
     CDS             complement(12638..13513)
                     /locus_tag="LNC71_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBK6465412.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13595..14386)
                     /gene="codY"
                     /locus_tag="LNC71_000315"
     CDS             complement(13595..14386)
                     /gene="codY"
                     /locus_tag="LNC71_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBK6465413.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14404..15804)
                     /gene="hslU"
                     /locus_tag="LNC71_000316"
     CDS             complement(14404..15804)
                     /gene="hslU"
                     /locus_tag="LNC71_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBK6465414.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15818..16366)
                     /gene="hslV"
                     /locus_tag="LNC71_000317"
     CDS             complement(15818..16366)
                     /gene="hslV"
                     /locus_tag="LNC71_000317"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBK6465415.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16403..17308)
                     /gene="xerC"
                     /locus_tag="LNC71_000318"
     CDS             complement(16403..17308)
                     /gene="xerC"
                     /locus_tag="LNC71_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBK6465416.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17506..18882)
                     /gene="trmFO"
                     /locus_tag="LNC71_000319"
     CDS             complement(17506..18882)
                     /gene="trmFO"
                     /locus_tag="LNC71_000319"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBK6465417.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18851..20929)
                     /gene="topA"
                     /locus_tag="LNC71_000320"
     CDS             complement(18851..20929)
                     /gene="topA"
                     /locus_tag="LNC71_000320"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBK6465418.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21051..21908)
                     /gene="dprA"
                     /locus_tag="LNC71_000321"
     CDS             complement(21051..21908)
                     /gene="dprA"
                     /locus_tag="LNC71_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBK6465419.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21964..22731)
                     /locus_tag="LNC71_000322"
     CDS             complement(21964..22731)
                     /locus_tag="LNC71_000322"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBK6465420.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22724..23584)
                     /gene="ylqF"
                     /locus_tag="LNC71_000323"
     CDS             complement(22724..23584)
                     /gene="ylqF"
                     /locus_tag="LNC71_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBK6465421.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23844..24242
                     /locus_tag="LNC71_000324"
     CDS             23844..24242
                     /locus_tag="LNC71_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6465422.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24521..25021
                     /locus_tag="LNC71_000325"
     CDS             24521..25021
                     /locus_tag="LNC71_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK6465423.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(25104..25655)
                     /gene="lepB"
                     /locus_tag="LNC71_000326"
     CDS             complement(25104..25655)
                     /gene="lepB"
                     /locus_tag="LNC71_000326"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6465424.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25807..26034)
                     /locus_tag="LNC71_000327"
     CDS             complement(25807..26034)
                     /locus_tag="LNC71_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBK6465425.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26031..26549)
                     /gene="msrA"
                     /locus_tag="LNC71_000328"
     CDS             complement(26031..26549)
                     /gene="msrA"
                     /locus_tag="LNC71_000328"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6465426.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26569..27282)
                     /locus_tag="LNC71_000329"
     CDS             complement(26569..27282)
                     /locus_tag="LNC71_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBK6465427.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27285..28181)
                     /locus_tag="LNC71_000330"
     CDS             complement(27285..28181)
                     /locus_tag="LNC71_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6465428.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28339..29181)
                     /locus_tag="LNC71_000331"
     CDS             complement(28339..29181)
                     /locus_tag="LNC71_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6465429.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29313..29966
                     /locus_tag="LNC71_000332"
     CDS             29313..29966
                     /locus_tag="LNC71_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBK6465430.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30029..30556)
                     /locus_tag="LNC71_000333"
     CDS             complement(30029..30556)
                     /locus_tag="LNC71_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6465431.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30576..31523)
                     /locus_tag="LNC71_000334"
     CDS             complement(30576..31523)
                     /locus_tag="LNC71_000334"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBK6465432.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31535..33421)
                     /locus_tag="LNC71_000335"
     CDS             complement(31535..33421)
                     /locus_tag="LNC71_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6465433.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(33581..35659)
                     /locus_tag="LNC71_000336"
     CDS             complement(33581..35659)
                     /locus_tag="LNC71_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBK6465434.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35672..35947)
                     /locus_tag="LNC71_000337"
     CDS             complement(35672..35947)
                     /locus_tag="LNC71_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465435.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36309..36764)
                     /locus_tag="LNC71_000338"
     CDS             complement(36309..36764)
                     /locus_tag="LNC71_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465436.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36918..38126)
                     /locus_tag="LNC71_000339"
     CDS             complement(36918..38126)
                     /locus_tag="LNC71_000339"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBK6465437.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38238..38570)
                     /locus_tag="LNC71_000340"
     CDS             complement(38238..38570)
                     /locus_tag="LNC71_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK6465438.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38718..39593
                     /locus_tag="LNC71_000341"
     CDS             38718..39593
                     /locus_tag="LNC71_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6465439.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39675..40256)
                     /locus_tag="LNC71_000342"
     CDS             complement(39675..40256)
                     /locus_tag="LNC71_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBK6465440.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40262..41521)
                     /locus_tag="LNC71_000343"
     CDS             complement(40262..41521)
                     /locus_tag="LNC71_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK6465441.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41523..42569)
                     /locus_tag="LNC71_000344"
     CDS             complement(41523..42569)
                     /locus_tag="LNC71_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465442.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42804..43079)
                     /locus_tag="LNC71_000345"
     CDS             complement(42804..43079)
                     /locus_tag="LNC71_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBK6465443.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43322..44632)
                     /gene="der"
                     /locus_tag="LNC71_000346"
     CDS             complement(43322..44632)
                     /gene="der"
                     /locus_tag="LNC71_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBK6465444.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44814..46043)
                     /gene="rpsA"
                     /locus_tag="LNC71_000347"
     CDS             complement(44814..46043)
                     /gene="rpsA"
                     /locus_tag="LNC71_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBK6465445.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46178..46858)
                     /locus_tag="LNC71_000348"
     CDS             complement(46178..46858)
                     /locus_tag="LNC71_000348"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBK6465446.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46907..47542)
                     /locus_tag="LNC71_000349"
     CDS             complement(46907..47542)
                     /locus_tag="LNC71_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6465447.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47608..49011)
                     /locus_tag="LNC71_000350"
     CDS             complement(47608..49011)
                     /locus_tag="LNC71_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBK6465448.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(48998..50029)
                     /locus_tag="LNC71_000351"
     CDS             complement(48998..50029)
                     /locus_tag="LNC71_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6465449.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50060..50281
                     /locus_tag="LNC71_000352"
     CDS             50060..50281
                     /locus_tag="LNC71_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBK6465450.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50446..51183)
                     /locus_tag="LNC71_000353"
     CDS             complement(50446..51183)
                     /locus_tag="LNC71_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBK6465451.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51336..52676)
                     /locus_tag="LNC71_000354"
     CDS             complement(51336..52676)
                     /locus_tag="LNC71_000354"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBK6465452.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52913..54436)
                     /locus_tag="LNC71_000355"
     CDS             complement(52913..54436)
                     /locus_tag="LNC71_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBK6465453.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54814..55425)
                     /locus_tag="LNC71_000356"
     CDS             complement(54814..55425)
                     /locus_tag="LNC71_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6465454.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55548..55673)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55755..56471)
                     /locus_tag="LNC71_000357"
     CDS             complement(55755..56471)
                     /locus_tag="LNC71_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK6465455.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56477..57046)
                     /gene="scpB"
                     /locus_tag="LNC71_000358"
     CDS             complement(56477..57046)
                     /gene="scpB"
                     /locus_tag="LNC71_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBK6465456.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57030..57827)
                     /locus_tag="LNC71_000359"
     CDS             complement(57030..57827)
                     /locus_tag="LNC71_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBK6465457.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57797..58168)
                     /locus_tag="LNC71_000360"
     CDS             complement(57797..58168)
                     /locus_tag="LNC71_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK6465458.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58187..59074)
                     /gene="xerD"
                     /locus_tag="LNC71_000361"
     CDS             complement(58187..59074)
                     /gene="xerD"
                     /locus_tag="LNC71_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBK6465459.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59170..59643)
                     /locus_tag="LNC71_000362"
     CDS             complement(59170..59643)
                     /locus_tag="LNC71_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6465460.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59748..60611)
                     /locus_tag="LNC71_000363"
     CDS             complement(59748..60611)
                     /locus_tag="LNC71_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK6465461.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60807..62906)
                     /locus_tag="LNC71_000364"
     CDS             complement(60807..62906)
                     /locus_tag="LNC71_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBK6465462.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(63126..64235)
                     /gene="rpoD"
                     /locus_tag="LNC71_000365"
     CDS             complement(63126..64235)
                     /gene="rpoD"
                     /locus_tag="LNC71_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBK6465463.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(64259..66100)
                     /locus_tag="LNC71_000366"
     CDS             complement(64259..66100)
                     /locus_tag="LNC71_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK6465464.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(66282..68597)
                     /locus_tag="LNC71_000367"
     CDS             complement(66282..68597)
                     /locus_tag="LNC71_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6465465.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68782..69642)
                     /locus_tag="LNC71_000368"
     CDS             complement(68782..69642)
                     /locus_tag="LNC71_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBK6465466.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69701..70222)
                     /locus_tag="LNC71_000369"
     CDS             complement(69701..70222)
                     /locus_tag="LNC71_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBK6465467.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            70500..71261
                     /locus_tag="LNC71_000370"
     CDS             70500..71261
                     /locus_tag="LNC71_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6465468.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            71287..73878
                     /gene="mprF"
                     /locus_tag="LNC71_000371"
     CDS             71287..73878
                     /gene="mprF"
                     /locus_tag="LNC71_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBK6465469.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(74066..75253)
                     /gene="tuf"
                     /locus_tag="LNC71_000372"
     CDS             complement(74066..75253)
                     /gene="tuf"
                     /locus_tag="LNC71_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK6465470.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            75621..75977
                     /locus_tag="LNC71_000373"
     CDS             75621..75977
                     /locus_tag="LNC71_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6465471.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(76218..77222)
                     /locus_tag="LNC71_000374"
     CDS             complement(76218..77222)
                     /locus_tag="LNC71_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBK6465472.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            77388..78239
                     /locus_tag="LNC71_000375"
     CDS             77388..78239
                     /locus_tag="LNC71_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6465473.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            78256..78501
                     /locus_tag="LNC71_000376"
     CDS             78256..78501
                     /locus_tag="LNC71_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465474.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT        21517 a        16521 c        13482 g        26984 t
ORIGIN      
        1 ggagatatta tggaaattca acgtagaacc actaattttt tacatttagg ctcttttttt
       61 gcaactttga cgatcttatt ttttctatgt tattcatacc ttaagacctc acatgcgcct
      121 tctagtgcat taggctggtt gcttttggat cattctgata tcacagaagc cgaagaaaat
      181 ccgttttttt tgattttgtc ttctctttgc ggcttactgt tttctgttta tttcggtgcc
      241 tatttttttc atcgacatcc tggtacgtta aacgaagctt caagcgtatt gattgcattt
      301 aaaacgtttt cttttttagc aattcttttt tattcgttta atcacaatac gcttttcttc
      361 tttatcatga atggtttagt cgtgatttta tccattttta cttattttat tttttcactc
      421 atttttagcc aattgaaatg tccgctcttc tttcgattag ttcctgtcac ctgttttatt
      481 tacagcttgt tttccttttt tgtccttgct ttaatgccac tgaccaaccc ttcgacgatc
      541 caagtcgcag gaaatctgtt ggatatgctc ttcatcgtca gtctaggtgt atttatgtgg
      601 caaagaaaaa acaaacaaaa taaaaagcat tttgaagcct gactattacg acttcataaa
      661 tgctttttta gttttaaaca cttcaatcta aaagaataac ccaagattgg ataaattatt
      721 aatgactcgc cattcgtttc ttagtgattc tggattatca aatggacttc ctaatctaat
      781 cagctagttc ttcccataaa atgccatact tgactgaatc ataatattga ttatttaaaa
      841 atctaacttt tcttatcact acttcctttg tcattccaca tttctcacca tttttttgca
      901 ttcctttgtt tccagaccat gtagtgaatc taataggtgg tagataggca aaatactcaa
      961 ataattcttt taaccataaa ggaagagaaa ggctcctcct gcaagagtca gtataatttt
     1021 tgcttactgc tctcttaggt tttactcact aaaactgttc catattcttt gatataagaa
     1081 aatgcatgcg gtataaattt cttgctcagc tcagaaaaga tttgtcgttt aagaaaggct
     1141 atttcctcca ttgtcaatga aggatccaat gttggtaatt caatctcaaa attgtttttt
     1201 tcgacaatag gtaattctaa tgtgaatgat tcttcttcct ttatagtatt cgtcacaagt
     1261 agagactgat ggaaaatact aggccggcga tttttatatt tattcttacg tatcctgtca
     1321 acgataactt ctatttgatc aaaaaagtac gattcattta cgtctccaat aagttcggct
     1381 aattctttga atgccttcgt tacaggaagc tgattcttct gtacaaaaaa gtaattagcc
     1441 aaagtaagcg ggaagtgttc tatagaggaa agtgtgataa aacggtactt ctcatttcct
     1501 gcagttgtcc tcagcaattc tgtctctcgg ctgtttctta ctccataaaa atacgcttct
     1561 ttccgttcat cttcgaaaca atacgcccaa ataacttctc ccatcgctaa tttttgttca
     1621 tcaacagaga gtctcttcaa ttgttcagct atggtttcag cagccgaagt ggcttgttgc
     1681 aagtaatctt taggatcaaa tattggaaag ttcaaatgat atcttcgttc ttccctcttg
     1741 attaatcctg catcgatcaa taaatcaaga aaatgggaaa atttattttg cgaaaattct
     1801 tttttcaatt ctcttagaat cgtttcttgt tctcgatgtt catatagata atcggtcaat
     1861 gaagaaaaaa gcggatcatc ggtcagtttt tgataaaagt tttccttatt ttttgaataa
     1921 tagttcatat atgttctctt ttctatccgt tttattttac ttaaaaggct tttacgtaaa
     1981 aaggaggaag aacaagagtc ataattgact gttattctcc tccatttctt cggcctttag
     2041 tatgttctat gtttcttttt atatcaatcg cttatccatt cattttgaat gcttcatact
     2101 accatttgat tagctaaata cagccgtcaa ttgaggtacg acttgttttt tacgtgaaac
     2161 gacaccttta agcaaagcac ggttgttggt caaagttgtt tggaaagctt cttctacttt
     2221 ttcttttcct ttaccagcaa caagtaattc tgaatcactg tctaagatgt ttgtgacaac
     2281 taatacgaac agatcgtagc catttgcttt catttcatct tccattgctg cttctagctc
     2341 tgcttgtcga tccatcactt cattaaggtc cactgtattg acttgagcga tacgaacgtt
     2401 ggaactgccc attgggaaac tttttgcatc caaatcaaga agaaccgcag ctgatttatc
     2461 acttagattt gttcctgctt tcaacatttc aagtccataa gtgtttagat cgacttcagc
     2521 gattgctgca agttcttttg cagctgcttc atcttcaggc gtacatgttg gtgatttgaa
     2581 caacaatgta tctgaaatga tagctgagag catgattcct gcgatttctt taggaattgt
     2641 tacttggttt tctttatata gttttaaaat aatcgtactt gtacatccaa ctggttctgc
     2701 acgatagtac aatggattag ctgtttcaaa gtttgcaata cgatggtggt ctactacagc
     2761 taaaatattt acttcgctga tatctgaaat actttgttgg aattcattat gatccacaag
     2821 catgacatct gcattttctg ctttagctga ctctactact cgtggtgcag atagctgaaa
     2881 gtggtctaat gcgtattgtg tttcttcatt aggtgtacct agagcaactg cttctgtttc
     2941 tttacctaat tcattttgca agtgtgaaaa cgcaatggct gccccgattg catctgtatc
     3001 tggattttgg tgaccaaaaa ctaaaatttt tgacatgtca ttcgctcctt tgtgatttct
     3061 cctatttaat aatagcaaaa aaacggaaag aagcaatact tatcttcttt ccgatgcgac
     3121 ctatcgcgtt aaatattttg tatactcttc tgtatgaagc aatcgtttag cattttcgac
     3181 acgttctttt gtaggtggtt cgatcccttc taatggatat ttcaaaccaa gtgtttccca
     3241 cttgtatttt cccatcgtat gataaggaag aatctctact ctgtctacat ttgacaaggt
     3301 ttgaataaag ttgtctagtc ggatcaaata ttcatcataa tcacttcgct caggaactag
     3361 tacatggcga atccaaaccg gtttgttgat ttttgacaaa tattgagcca tttctaaaat
     3421 attttcattg tcccaatgcg ttagtttttt atgctcttca ttatcgatgt gtttgatgtc
     3481 aaataacagt aaatctgtat acttcatcaa ttcttgaaaa cgtgaaaaga aaggttcttc
     3541 atacgtaaaa ggttttccgc atgtatccaa tgtagtatgc atcttccgct cttttgcttt
     3601 tttaaatagt tcgatcaaaa aatcgatctg caatagaggt tctccaccac taacagtaat
     3661 accaccttta tctccccagt aaggtctgaa gcgttctgct ttatctaaaa gttcatctgc
     3721 tgtgtattct ttccctccgc caatattcca agtatctgga ttgtgacaaa attggcaacg
     3781 catgcggcat ccttgcataa acacgacaaa gcgcagtcca ggtccgtcta ctgaaccgaa
     3841 agtttcgata gaatgaacat agccgattgt tttttcttcc atttaagttc acatcttttc
     3901 tttgatttca ttttaagtac aagttagctg tcgaagcgaa gctttctgaa caaaattttt
     3961 cgcaattgcc gcttcgttat gtgtaaaatt tacagggacg gaacatacaa gtcctgcccc
     4021 tgtaaatcgt ttctttcaaa taagcatact gattattcga ttttgatgcg atggctattc
     4081 aacgtccttt cgacgctaag tcgaaaaact tttttcacga actttttaca ttctgtcgtg
     4141 tgaagttctc atcaagacat ctaattgttg ttctcttgtc aagtcacgga atttaactgc
     4201 ataaccagat acccggatcg tcaagttcgg atatttcatt ggattttcca ttgcatcacg
     4261 caataaatca ttactgaata cattgacatt caaatgataa gctccttttg agaagtatcc
     4321 atctaagaca ttacgcaagt tttcgattct tgtatcttgt tcttttccaa gacctgaagg
     4381 attgattgtt tgggtatttg aaattccatc caatgcacag ccgtaattca ttttagctgt
     4441 tgagttcagg ctagctaata acccactttg ttccgcattg taacttggat ttgctcctgg
     4501 cgcaagtggt tttccagctt cacggccatc aggagtattt cctgtatttt taccgtaaac
     4561 aacatttgaa gtaatcgtca ataatgaagt tgttggtttt gagttgcggt aagttttatg
     4621 acgtttgatt tgtcccatga agtattccaa gatccatgaa actaattcat ctgcacggtc
     4681 atcatcattt ccgtatttag ggaagtcgcc atccacttca aaatcaactg ctattccttt
     4741 ttcattacgg attgctttta cttttgcata tttgatcgct gacaagctgt ctgccgcatg
     4801 agagatccca gcaatacctg tagcaaatgt gcgttgcaag tcagaatcca tcaaagccag
     4861 ctgagctgct tcataagaat atttatcatg catataatga atgatgttca acgtattgac
     4921 atatagttct gctaaccaat ccatcatatt catgaagtta tgtttcactt cttcaaagtc
     4981 tagtatttct gatgtgattg gacgatattc tggtccaact tgggcgccag tcacttcatc
     5041 gactccgcca ttgatggcat ataatagtgt tttagccaag ttagcacgag cgccaaagaa
     5101 ttgcatgtct tttccgacaa ctgttcctga tacacagcaa gcaatcgcac aatcatcaga
     5161 accccattgt tcacgaagct gattatcatt ttcgaattga atcgaagaac tttcgatagc
     5221 gattctgcta gcatatgttc tgaatccttc tggaaggtgt tcagaataca aaacagtcaa
     5281 gtttggttct ggtgaaggtc ccatatttgt taatgtatga agcagacgga aatcattctt
     5341 tgtaactaat gaacgaccat ctaatcccat acctgcaatg gataaagtag cccagatagg
     5401 gaatcctgag aataattggt tatattcagg tgtacgcgcg aatttgacca tacgtaattt
     5461 cattactaag tggtcgatca attcttgtgc ttctttttct gtgattactc cattatccaa
     5521 atcacgttgg atatagatat caaggaatgc tgagatacgt ccgattgaca tcgcagcacc
     5581 attttgtgat ttgattgctg ctaaatatcc gaagtatagc cattggacag cttcttttgc
     5641 atttgcagct ggtttagaaa tatcatagcc atatttcgca gccatttctt tcatttgcgc
     5701 taatgcacga tattgttcag tgatttcttc acgtaaacga atcacttctt cactcattgt
     5761 atgataacca gtcatatcat gatctttttt cttttgttcc atcaaatagt cgattccata
     5821 caatgccaca cgacgatagt caccgatgat acgtccgcga ccgtaagcgt ctggaagccc
     5881 tgtaatgatt ttgtttttac gagcagcacg catttcagca gtataagcgt caaatacacc
     5941 ttgattatgt gttttgcgcc agtcagtgaa aatatgtgtc aattcatcgt ttggtgtata
     6001 gccattgctt tttagcgcat tgttagccat attgattccg ccgaacggca tgaatgcttg
     6061 tttcaaaggt ttgtctgtct gtagaccgac gattttttcc aaatctttat ccaaatagcc
     6121 tgcatcgtgt gaagtaatag ttgatacgat ttctgtgtcc atatcataga caccgttacg
     6181 ctcgaattgg atatcattta acttttgcaa ttcatcccat aatttttctg ttgcttctgt
     6241 tgggccttcc aaaaagctgt catcaccgcg atattcagtg tagttatttt gaataaagtc
     6301 tctagtgtct acgccttctt tccatttggt acctttaaat ccattccatt gctccattgt
     6361 atggacctcc taaatttctg ttttatgtaa cagtttttac gtgattagta taacacagca
     6421 ccctatcttt gcaagggttt tcttgttttt tatttaacaa tttttatgtt ttatttacat
     6481 atatttaaat caacgttgac ataccaacat ttaagcattg tgaacacttt catctttttt
     6541 tgcaaacgta taaggattta ttaaaaatga atgaaaaaga gacgccacct gtattaatac
     6601 agatggcgtc tcttcattaa aatactacaa cagttacgac tcgatgaaaa tctcaggagc
     6661 atcgataact tcgatgactt ctccaccttt tttctcatcc atcgcaaatg atccattcga
     6721 tgttcgatca cctataggga aatcagttgg gtcaatcgtt aactgctgcc ctttttgatt
     6781 catcaaggtt atttcttttg atcttccttc cgacatatag ataatacggt gcggattttt
     6841 tttcagctca cgcatgaccg tcactccccg cttcgcacgt gaagtttggc tgatttcttg
     6901 agcaagcatg cgtttaacag ctcctcgctg agtaacgatt acgatcggcg tatcaccttc
     6961 tgctaacaca agcagcccgt tgataatcct atctccatct ttcaaattca tagacttcac
     7021 tccagctgcc tttgctccaa caacgggaac atctgttaaa ggatagcgca aacccattcc
     7081 ttgatgcgtt actaagaaaa catctaagcc tggctgatct tctttcagat acacagcaat
     7141 cagttcatct tcttccgcct ttagcttcat acagctgagc ggacggctct tataggtgcg
     7201 ccatggttcg aactctgtca ttcgtgtttg cttgatcaat ccattcttgc tgatgaatac
     7261 aaatgttttc tctgcttgaa ttttttgata tgggaaaaca gctaaaatcg attcgtctac
     7321 agacagattg gagattgttt gagaaatgtg ctcaccagca tctttccatt tcaaatcggg
     7381 tagttcatgg acagggcggt agatcacatt tgctttattt gtgatcagaa gaacatggtc
     7441 caatgtactc aattcacctg agtatagcaa gtagtcgcct tctcgcatcc cgatttcttc
     7501 tggtttggaa gcactataag aacgaaggct tgttcgcttc acatagcctt ctcgcgtcac
     7561 actgacaatt acgtcttctt gtgccactaa gacttgcgta tcaattttga tttcttcgat
     7621 ttcgtcttcg attgtcgtta gacgagcgga agaataattt tttttcactt ctcgcaattc
     7681 ttttttcata acagagaaaa gctctttgtc atttgaaagg attttattca actctgtgat
     7741 ttgcgcaatc aagctttcgg attccttttg aagctctgtg atatccgtgt tcgtcaaacg
     7801 gtataattgc aatgtgacaa ttgcttctgc ttgttcttct gtaaattgaa agacgtcgac
     7861 taaattcttt ttggcgtctt ttttgtcttt actttcacga atcgttgcga ttacttcatc
     7921 cagaatcgat aaagctttca tcaatccttc tacgatatgc tgtctttttt gagctttggc
     7981 taaatcgaat tgactacgtt ttgttatgac ttgctttcga tgctcgatat aacttcttaa
     8041 aatgtctttc agtccaactt gatgcggagt catatggtcg atagcgacca tattgaaatt
     8101 gtaattgatc tgtagctcag tgtttttgaa caaataattc aaaattcctt gagcattggc
     8161 gtcttttttc aattcgacga caatttgcag acctgttcta tcactttcat ctcgtacttc
     8221 agctattcca tcgattttct tattcaaacg gatctcatcc atttttttca ccaaggtcgc
     8281 tttatttact tcatatggga tttctgtgat cacgatttgc tgtttattac ccttcattgc
     8341 ctctatcttt gttttcgaac gaagaataac ttttcctcgt cctgtttcat aagctttttt
     8401 gatttcatct tttccttgca agatccctcc tgttggaaag tcaggtccag gaatatattc
     8461 catcaatttt tcaaggctcg cttgtggatg atcgatcata tagattgtgc catcgatcac
     8521 ttcagctaag ttatgagtcg ggatttctgt tgcatatcca gcagatatac ctgtcgatcc
     8581 attgacgaga aggtttgggt atcgagctgg aaggactgtt ggttcttttt ctgtatcatc
     8641 aaaattccac acgagatcga ctgtttcttt ctcgatatct gctagcatct ctccacttaa
     8701 cttggataat ctggcttcag tataacgcat cgcagcaggt ggatcaccgt ccatactccc
     8761 gttattcccg tgcatttcga tcagtacttc ccgcaacttc cagtcttggc tcatgcggac
     8821 catcgcttca taaatactga tgtcaccatg aggatgataa ttccccatga tatttccgac
     8881 agattttgct gatttacgga atcctttgtc atacgtattg ccatctttgt tcatggcaaa
     8941 taatatccgg cgttgcactg gcttcaatcc atcacggata tcaggcagtg cacgttcttg
     9001 gataatatat ttggaataac gtccgaagcg gtcacccatc acttcttcta aagttaattc
     9061 ttgtacttct tgtcgatttt ccatcaggtg acctcctatt ctacttcaaa taactgattg
     9121 ttttcatttt tatctgctcg ttcttcatct aataaatcat tagaaactga aggagagatt
     9181 tcagtatcct cttttttatc taagatactg ccatcttctt cgagcgtaaa ttgcacgtga
     9241 ttttcgatcc attttcttcg aggttctact ttgtcaccca tcagtgtggt tactcgacgt
     9301 tcagcttgcg cagcatcatc gatacggacg cggatcaacg tacgggaagt cggatccatc
     9361 gttgtttccc acaattgttc cgcattcatc tctccaagtc ctttatatcg ctgcagcata
     9421 tatcctttgc cgactttttt aatcattgct gccaattcgt catctgtcca cgcatattca
     9481 atgacttgct tctttccttg tcctttggat actttatata aaggcggtaa cgcgatatat
     9541 actttcccag cttcaatcag cggtttcata taacgataaa agaaagtaag cagtaatact
     9601 tgaatatggg cgccgtctgt atccgcatcg gtcatgatga ttactttgtc gtaattacag
     9661 tcttcgatag aaaactcagg accgactcct gcaccaatcg tatagatcat cgtattgatt
     9721 tcttcatttt tcaagatatc ttgcatttta gctttttcgg tattgatgac ttttcctcgt
     9781 aaaggtagaa ttgcttggaa ctttctgtct cttccttgtt tagcagatcc gccagcagaa
     9841 tccccttcga ctaaatacaa ttcatttttc ttagggtttc gggattgtgc gggtgtcaat
     9901 tttcctgaaa gaagagattc gcctttttta cgcttcttcc catttcggct ttcttctctg
     9961 gctttacgtg ctgcttctct cgcttctctg gctttgattg ctttacggac caacatctgg
    10021 ctcatttcgc tattttcctg caaataaaag cccatttgtt cactgatgac gttatctact
    10081 acggtacgag caactggcgt accaagtttt tcttttgttt gcccttcaaa ttgcagaaga
    10141 ttttcaggta cacggataga aagtacagca gctaaccctt ctctgaaatc actaccttcc
    10201 agattcttgt cccgttcttt cagcaaacca acttttctcg catattcatt ataagatttc
    10261 gtcatcgccg ctttcatacc agcttcatgg gttcccccat cttttgtccg aacgttattg
    10321 acaaaagata aaacattttc tgaataaccg tcattgtatt gataagcgac ctctacttcg
    10381 ataccttctt tttctcctga aaaataaaca actggtgtca atgtatcttt ttcttcattc
    10441 agataatcaa caaattcttt gattccttct tcgtaatgaa aaatttcctt gactggttct
    10501 tctccacgaa gatcagaaag agagatttta acgcctttta gaagaaaggc agattctctt
    10561 aaccgttcgg ctaaaatttc atatgaaaat ttcgttgtac tgaatattgt atcatctggt
    10621 aaaaaatgga cagtcgtccc gtttgctttt tttgtttttc ctatcttttt caaagtaccg
    10681 tcaggtttac cgccgttttt aaatttctgc tgatactcta cgccatctct aacgatcgtt
    10741 acaattagcc atttcgataa ggcatttacc acactggctc ctacaccgtg cagtccacca
    10801 gaagttttgt accctccttg accaaacttt ccgccagcat gcagcacagt aaaaatcact
    10861 tcaactgttg gtattccgga agcatgcatt cctacaggca tcccacgtcc gctatctgca
    10921 acagttatac tattgtcttc atggatggtc acatcaatct cactgccata acctgataat
    10981 gcttcgtcaa ctgcattatc gacgatttca tagactaaat gatgcaagcc tcggctgtca
    11041 gtggatccaa tgtacatccc tggtcttttt ctaactgcct ctaatccttc caatacttgg
    11101 atggacgagt cattgtattc gttgtttact ttttttgcca aggcaaacgc tccttcaata
    11161 atactttgcg gcaaaaccgc accacgatta tcatactaga aaaggtcgtg aaaaaaaaga
    11221 gggtagacga gaaatcaatt ttaattatct cctaaccgct ttttcgacat tcccctctat
    11281 cgatcatctc ttctttgtgg tcatttttat ttttggtttt ctttcaactt accaagttca
    11341 atcttaatac aacgatccat tacgatatct gtacgtcctg cttttcttag catctcagct
    11401 gcttctttgt tttcaatccc taattgtgcc cagaacactt tagcatcagt tttcaaaaaa
    11461 tcacctgcta tttcaggtaa aaattcactc cgtctaaata catctacgat atcgattgga
    11521 tcatcgattt ctgtcaaatt cgcatagact ttctcgccta gaatttcttc accagccaat
    11581 aatggattta ctggtatgat tttatatcca ttgtcttgca atacctgtgc aaccatgtaa
    11641 cttgttcgtt ctggtttatt gctcaagcct acgacggcaa tattttttgc ttcttccaaa
    11701 tagttgaaaa tcgtttcttg tgttggattt tttacagaca tttctgccac cccttgcttt
    11761 tctttttatt ccatctatta tacttgtatc tttcattttt tcaattgttc ctgttaccta
    11821 ttcatgctaa aataaaggat agttttcaac ttgaaagagg gtaaaaaatg aaattgatct
    11881 tattacttat tttagcatat cttttaggtt ctattccttc cggtgtctgg attggcaaat
    11941 tgtttttcaa aaaagatatc cgacaacacg ggagcggtaa tactggaacg accaatacat
    12001 ttcgtgtatt aggcaaaaca gccgggattg ttgtcctgct tatggatatt ttaaaaggta
    12061 cattggctac ttgtttgccg atgatcttcc acctttctgg gattaaccct ctttggtttg
    12121 gggtatgcgc cattcttggc catacatttc cgatttttgc tgggttcaaa ggaggaaaag
    12181 ctgttgcgac aagtgcagga atgcttttag gatttaatcc tgtatttttt gtttattctt
    12241 gtatcatctt tatcgttagt ttattttgta caagtatggt gagtttgagc agtatgatca
    12301 gtgcagtatt gatcacttta tctacgatcg tccttcctta tgtagctcct gtgattttgg
    12361 ctaagcccaa ttggttactg acgatcattg cctttttagt ttcttccttt atcttttatc
    12421 ggcataaaga caatatcaag cgtatcagaa accatactga aagtcgaatc ccgtttggtt
    12481 tgaatagcag caagaaaaaa tcctgatact ttttcattcc aaagagataa aacgggagtg
    12541 tgacaaatcg atgtcacact cccttattac ttacaggata gcttttttac acaccttggt
    12601 aaacggtgtt ccctgctaac ttctcggtca caacctctta cttcactgtg atggaatatt
    12661 ttgtcttgaa gatttcatgg gcacctagtt gattgatccc tactttttcc atcaagtttc
    12721 cactactgtt caccgtgtca gcgattccgc accacggttc gatacaaacg aaaggcgctt
    12781 cttttggata aggagaccag atccctacat aaggcatatc tttatagctt aaagttacgc
    12841 tatgggaaga tttttcactt cgaatggcgt acgcatttag accccttgtt tcaaaaacca
    12901 aggcatcttg ctcaaaaagt tcacgattca aagccagatt cgtatttgtc tgacctaacg
    12961 tcttttgatc catatcgatg aatgggcctt tcaatggtag gcgtaatctt gatttcaatg
    13021 gagaaaatgc cagatagaaa tcttcaaatg acaatccttc ttcaagaggt acattgaaag
    13081 caggatgtcc accgatagaa aagaacattt ctttttcacc ggtatttgcc acttgataat
    13141 gaaccgtgat gccgtctcct cctaattcat agctgatcac taattcaaaa tcaaatggat
    13201 acttttctaa tgtctccggc gtactcctta agatgaaact agccatttct tgtccatgtt
    13261 caatgacttc aaaatccata tcccgagcaa atccgtgttg ccccatttga tatgttttcc
    13321 cttgataaac atactgatca tcttttaaac gtccaacaaa agggaataaa accggtgcat
    13381 gtctgcccca atacgctgga tcaccttgcc agatatactc taaaccatgt tcttttgatt
    13441 tcagactgat caattccgca ccgtgttcag cgatttcggc gatcagctgg tcattttgaa
    13501 taataacagt cattgccatt cctccaactt cgacttactt tttaaaatgg gaaggctgga
    13561 acaaacattt ctatctgccc cagtcccctt acttctaata actgtgtttt gctaactcgt
    13621 ctttaaaacg ggaattcaat acttttaaat aagttccctt catgcctaac gaacgagatt
    13681 caatgattcc agctgattcc aattttcgta aagcattgac gatcacagaa cgagtgatgc
    13741 cgatactatc ggcaatatta gaagcagtca gacgaccttc atctccatcc aatgcttcaa
    13801 agatagcttg tacagccttc aattcgctat aagataatgt gttgattgcc atttggacag
    13861 ccgttgcact ccgtacatct gcttcgatgc ttcgagactt ctgataaagg atctgcatac
    13921 cgacaaccgt agctccatat tctgctaaga ctagatcgtc atctgcaaac gattgttcca
    13981 tacgagataa gataatcgta cccaaacgtt cgccagctcc aaagatcgga acgacagtcg
    14041 tcagaccaaa tggatatttc tctcttgact ctaccggaaa tgcagtaata tcactagtaa
    14101 ttgggatatt agcttcagtt ttcatcaact gtgccactgt atcagtataa gattcaggaa
    14161 attgtttctc aacaaacatt gttttgattc gttcattgtt cacatcgtga cgttcattat
    14221 aacctagtaa tactccgtca cttcctatga tataggtgtt gctgtcaaga ataccaccta
    14281 gtgtgacagc cattttatcg taaggcaaat ctgcttgcat gtcaaaagta ttcttttgct
    14341 gcaaaagtgt gtttatcttg cgggttttcg ttagcaagtc tgtcattgtc gtcctccgat
    14401 tttttataaa atatagcgac ttaaatcttc atttgccgca attgagccaa gtttgtcatc
    14461 cacatatgct tctgtgatcg ttatttctcc catttgcata tctggtgctt caaataatag
    14521 atcttctagt agtttttcca aaatggtatg cagtctgcgt gcgccgatat tatctgtgtc
    14581 acggttgaca tcgaaagcga tttgagcgat ccgttcaatt gcttcttttg taaacgtaac
    14641 tgacacattt tctgtaccga tcaacgcgac gtattgtttt actaaagcat tatttggttc
    14701 ggtcagaatc ttcacaaaat cttcagctgt caaatcatct aattctaccc gtatcgggaa
    14761 gcgtccttga agttctggaa ttagatcact tggtttagac aaatggaaag ctcctgaagc
    14821 aatgaatagg acgtgatctg tttggatgat cccgtatttt gtgtttactt gtgaaccttc
    14881 gacaattggc aatatgtctc tttggacgcc ttctctggat acttctccag attgctgtga
    14941 tttagaagtg atcttatcga tttcatcgat aaaaataatt ccactttctt ccgctaattt
    15001 gattgcctca ctatggatat cagcatcctt gactaatttt gctgattctt ccttgatcaa
    15061 taactctaaa gcttctttga ctgttaccgt ccgttccact tttttcttcg gtgtcaaagc
    15121 acctagagtt tcatttaggt cgatccccat ttgctccaat ccattgttca tcattggtgc
    15181 aggtttcttt tggtcttcaa cttcaatggt tacttcacga ctatccagta aacccttttg
    15241 tagttgttcc aagactgtac ggcgattgat tcgaatatct tctgtcactt cttcttgcgg
    15301 ttcagcggta ttttgctgac tagcattaaa aagattcatc atttgttcaa attgattagt
    15361 ggattgtttc tgttcttttt tgataccagg taccaagatt tttaccaaac gtttttctgc
    15421 atttttttta gcacttgtgt atacacggct gtactgctct ttttctacga tttggatcgc
    15481 attttctact aaatcccgaa ccattgattc tacatctctt ccgacataac cgacttcagt
    15541 aaattttgtt gcttcgactt tgacaaatgg tgcattgacg atttttgcca aacgtcgtgc
    15601 gatttctgtt ttaccgaccc ctgtaggtcc gatcatcagc atattttttg gtgtaatatc
    15661 ttgttgcatt ttttcatcta actgcaaacg gcgatagcgg ttacgcaaag caacggcaac
    15721 agattttttt gctgcgtgct gtcctacgat atattcatct aattcactta cgatttgtct
    15781 tggtgtttgt tttaattcat acatatctac tcacctctta cagttcttct acgataatat
    15841 tatgattagt aaagatacag atatctgcgg caatatttaa cgcattttct gcaatttctt
    15901 tcgcactcat gtcgttttga gcatagtttt tcattgctcg ggctgcagcc aacgcaaaat
    15961 tccctcctga tccgatagct aagataccat catctggtgt gataacttct cctgtacctg
    16021 aaacaagaag catttcttta tcattcatta cgatcagcat cgcttcaagt ttttgcatag
    16081 attgctgtgt ccgccattct tgagctaatt cgacagctgc gcgttgtaga ttcccgttat
    16141 attcgtttag tttgccttca aatttttctt ctaaagtaaa cgcatctgca acacttccgg
    16201 cgaaacctac aacaacttct ccattgtaga tacgtcgtac tttctttgca gtgcctttca
    16261 tgacaacaga ttcacccatt gttacttggc catctccagc catagcaaat ttcccgtctt
    16321 tttcgacggc acagatagtg gttgaatgaa atggtgattc gaccatgttt gtttcctcct
    16381 aaatttgttc ttttccttat ttttacgctc ttggatggaa agaacgatag tttttttgta
    16441 aactttcttt tgttacatgg gcatagatct gggtagtcga taaattggca tgtcctagca
    16501 attcttggac tgtccgcaaa tctgccccat tatttaataa atgagtagca aatgtatggc
    16561 gcagcatgtg aggatggata tcactatcca aactgctttt tttgatcatt tgattcaaga
    16621 catattcaat tccagtaggc gtgattggtt ttccatgatg attcacaaaa acgatatcat
    16681 gctcttcgtg atattttgtc atcaatacct ggcggccttc cctgaaatat tcttgcagag
    16741 catcttgagc aaaagaacct agaggagcat atctctcttt gttcccttta ccatggatca
    16801 acatgacctc tgaatcaaaa tcaatgtctg ataccgacaa actgctacac tcacttaaac
    16861 ggatgcctga cccgtaaagg acttccagta aagcgcggtt cctcaaatcc aaaggactat
    16921 ctcctgctac gctgtcaaaa agtgcttgca tttcattttc ataaaaaaaa cgtggcagtc
    16981 tcaaattttt ctttttgaga tgaacgtaag aaaatggatt ttcttttatc acttcttctt
    17041 tgagaagata ctgataaaac gaacgtaggc tggctatctt gcgactgata gaatttctgc
    17101 tatagctagg acgatgatca gctaatgcac tcaaatagac acgaaccgta aaatattccg
    17161 ccttcagcag atcagcgtct ccagattcct ccaaaaaatg tttgaaatgg agaatgtctt
    17221 cttcatatgc ttctttcgtt ttttctgaat atcctctttc gacaatcaaa taacgcaaaa
    17281 atttttgttc ccacgtggct tctgtcattt tctcaccttc cttttgtccc agagaataat
    17341 ttagcacagc gtttataaaa agacaaataa attgtcagaa tttaaccaat atttctaagt
    17401 ttgttcacaa ttggcgcaat tcctcacatt ttgtttgaga aattcttaag aaaaacgcaa
    17461 gaaaaagagc gagactatcc gtctcaccct cttcatggag tttccttact catttgggat
    17521 tgcagttttt tctaattctg tttttattgc ctcattagct tttaaagctc gttgtgcaat
    17581 tgcttcatat cttgcttttt tgtctcgaat gcgctctggc agttcaggaa acagaccata
    17641 gttggcattc atcggttgga aatgttttcc ttccgcatga gtaatgtagt aagccatact
    17701 tccaatagct gtttcttgtg gaaactcgac tggttccatt tcttttgcta agcgtgcggc
    17761 gttgattcca gcaagtagtc cgctcgctgc actttcaaca tagccttcta caccagtcat
    17821 ctgacctgca aagaacagat cttcgcgttt cgttgattgg taagtcggtt tcagcaattc
    17881 tggcgaattc ataaagctgt ttcggtgcat cacaccatag cgaacgaatt cagcattttc
    17941 taacccagga atcatccgga aaacacgttt ttgctcgccc catttcaagt gagtctgaaa
    18001 gcccacgatg ttgtataaag aagctgcagc attgtcttga cgcagctgga taacagcata
    18061 aggacgtttg ccagttttag gatcttccaa tccaactggt ttcataggtc cgaaaagcat
    18121 cgttttgaat cctcgctgtg ccattacttc aattggcata catccttcaa aatatttttc
    18181 tttctcaaaa gatttaagtt ctgccacttc ggcattgatt agtgcctcat ggaaagcttt
    18241 gaattcctct tctgtcatcg gacagttcaa gtaagctgct tctcctttgt catagcggga
    18301 cttcaaatat actttatcca tatcgatcgt agctttgtct acgattggtg cagctgcatc
    18361 atagaaataa aaaccatctg aaccattgaa tgctttgatg ctttctgcta atggatgaga
    18421 agtcaaaggt cctgttgcta ctacggtgat tccttccggt atttcagtaa tttcttcgtt
    18481 tatcactgtt actaatggat gctctttgac ttttcgggta attgtttcag aaaaagaatc
    18541 gcggtcaaca gctaaggcac caccagctgg cactgctgtt tcatctgccg aagtgattac
    18601 taccgaattc aatcggcgca tttcttcttt caataaccct actgcattag ccaaactgtt
    18661 tcctcttaat gaattggaac atactagttc agcaaaatta cttgtgtgat gcgcctcagt
    18721 cgatttgact ggacgcattt catataaacg gacagggact cctgcttgtg cgatctgcca
    18781 agcagcttca cttccggcaa gccctgctcc aacaacattt accattttca atgaaagaac
    18841 ctcttttcta ttatttttgg atgtcttctt cgtaatctcc atttacacag actacttgtt
    18901 taccgccttt gactttcttc tcgaccagat atccgtcaca ttttggacaa tttctgccca
    18961 ctggtttatc ccaagaagtg aattcacatt ctgggaaacg ggaacaacca tagaatagtc
    19021 tgtttttctt agattttcgt tcaatgactt gtccttcatg acatactggg caagtcacac
    19081 cgatttcttt tacgattggt ttcgtattcc gacaatcagg aaaattactg caagcataaa
    19141 acttcccata tttccctaat tttatcacca tcggatgtcc acagacatca caatcgaatc
    19201 ctgctggttc atctttgatt tgaatttttt cgattttttc ttccgcattc gtcagttctt
    19261 tttcaaacgg tttatagaaa cgatcaacca ctttgaccca cgcttccgtg ccttcttcaa
    19321 ttttatccaa atctccttcc atttctgctg tgaaatggat atcgacgatt tgagggaaga
    19381 attcacagat taatgaattg acaatttctc ctagttcagt cggctcaaat cttttttgtg
    19441 tcaattttac atagtagcgt ctttggatcg tatcaagtgt tggtgcataa gtggaaggtc
    19501 gccctacccc gttttcttct agtgcacgaa tcaacgtagc ctcactaaaa cgtgctggtg
    19561 gttgagtaaa atgttgtttt ggttcaatat caacagattg gactacatcg ccttcttcaa
    19621 gttctggaag aatattttct ttctcttctt tgccgtcatc tctaccttcc acatacactt
    19681 gcatgaatcc tttgaatttg atttttgaac cgttcgcaat gaagatgaca ccattttgtt
    19741 ctaatgtcac tttcattgta tctaaaatag ctggtgtcat ctgactagcc acaaaacgtg
    19801 accagatcaa tgtataaagt ttcaattgat ctttatctaa atattgtttg agttcatctg
    19861 gtgtacgaag cacactcgtt ggacgcacag cttcatgggc atcttgtgcg ccttgagcat
    19921 ttttgatctt tcggtgatca tgtctagaaa attcttttcc atacgtatct tcaataaatg
    19981 ctgcggcctc tgcttttgct gtatcggcaa tccgggtaga atcagtacgc atgtatgtaa
    20041 tcaagccgac tgttccttgt ttacccaaag tgattccttc atataattgc tgtgctacca
    20101 tcatggtttt tcttgttcga aagtttaact tacgggcagc ttcttgttgc aggctacttg
    20161 tagtgaaagg attcgcaggg tttcttttgc gctctttctt ttctactttt ttaacttcga
    20221 agtctgttcc atcaattcta gcagtgactt cttttacact ttgtgcatct ggtaatttct
    20281 ttttcttacc gtcgattccc cagaaatttg ctttgaattt cttgcgagct tttttaaaat
    20341 taccgtcgat gctccaatat tcttctggaa caaattctcg gatctctttt tcacggtcaa
    20401 taataatttt taaagcgaca gattggacac gtcctgcact taaccctttc ttgaccttac
    20461 gccagagaat aggactgatc gaataaccaa cgattctgtc taaggtacga cgagcttgct
    20521 gtgcatctac taaatctaca tcgatcgttc ttggttcctt aaaagctgct ttgactgcgt
    20581 cttttgtgat ttcattgaag accacccgat ttttttcttt cagatccaac ccaagtaggt
    20641 acgctaaatg ccaagcaatc gcttctcctt ctctatccgg gtcacttgcc aagtaaactt
    20701 tttgagcttt ttttgctgcg gctttcaggc ttttgatgac atcgcctttt ccgcgtatag
    20761 aaatataatg gggttcataa ttgttttcaa aatcgatacc cattttactt ttcggtaaat
    20821 ctcttatatg gccaacactg gccattactt tatagtttcg tccaagatat ttctcaattg
    20881 tcttggcttt tgcaggtgat tctacaatca ctaaatattt atacgccatt actgaatgga
    20941 ctcctttcca ttgtcgtgtt cctactatta aaagaggatt catacaaatc gtagctaatc
    21001 atatccattc agaaaacgtt tgtcaagaaa agcaaatgaa aagtatggtg ttattcttta
    21061 aattctgcca agatatcttc acccgaaacc gtgcaaactg caccgtcttg gatcaaatga
    21121 tgacatccgg tagaatgttc tgttaaaata tttcctggaa cagaaaaaac tgttcttcca
    21181 agatccaatg cttgctgtgc tgttatcaga gagccgcttt ttttcttggc ttcaaagaca
    21241 catgttcctt tagacaaagc tgcgataatc cgatttctca ttggaaaaca atgctttttc
    21301 gggccggatt gaggaggata ttcactcaaa agaagatgat catttgcaat cacctcttgc
    21361 aagtcacgat tttcagcagg atagacttgc tctatccctg ttccaatgac tgcgattgtc
    21421 cgtccaccat gagagattgc tgcttggtga ccgtatgtat ctatcccttt cgcgcagcca
    21481 ctgacgatca catatccagc ttctatcact ggttttagta aataataaac tgtttttttg
    21541 gctaaagcag aagtgtgtct tccaccaaca acgcttaaca tgttacaaga aagtaaagat
    21601 agatcaccat tataaaacag tacaattggc ggataggtca agtgaagcag ctgaggggga
    21661 tatttgggat caaagcaagt gataaacgac tgtttcgatt ggattttgcc ccagttagcg
    21721 tgaatttcct gccaactttt gcgaaacagc tcccggtgtt tggttatttt acctatctta
    21781 atgatgtctt caaatccgat ttctttataa ggatactgca tcatccagtt agcaagtgcc
    21841 cattttcctt gactactgat tccttttgta taagcaagtt tcaataaatt ttcttctatt
    21901 tgatacatca taaaaccgcc tttcgtctct ctttctaata agatacgtaa aagcggctgt
    21961 tttttattgt aaatattttt ttacaggtgc aaaactttta cgatggattg gcgtaattcc
    22021 ttgtcgttca agtccttcta agtgttcctt tgttccataa cctgcattat gcgagaaacc
    22081 atatccagga taaagcacgt catattcttt catcaattgg tctcgatata ctttagcaat
    22141 gatacttgca gcagcaatcg acacagatcg tgcatcccct ttgattattt tttcctgctg
    22201 aataggcaaa tccaaggtca tcgcatcgat caacaaatag cttggtacaa tggacaattt
    22261 ttctactgct tggatcatcg ctaatttact tgcttcataa atattcacat catcgattgt
    22321 ctgctgatca acgatcccga tcccgattgc cagggctttt tcttgaactt gttgataaag
    22381 aagttctcgc tttttctctg ataattgttt ggagtcattc agccctaata tgatctcatt
    22441 tttcggcaag ataactgcag ctgccacgac aggtccagct aatggtcctc ttcctacttc
    22501 atcaatgccg gcaatccatt ggtgaccttg tgcgtaccct tgcttttcaa agcaaaacat
    22561 ttcttcatat ttttccttaa ttaatgcttc ctttttcaat cgtcgttcaa aagaagccag
    22621 tgcttgctgc acgcctttgc gtggatcatt tcttaatgat tctagtgtag gatcatctat
    22681 tctctgtatg cttttcaatc gttctttgat actttggatg gattcagtca tcactttttt
    22741 gctccatttc ttcgcaacga tccagtgtgt atcgtccaag ttttccttga cgaatatcat
    22801 gcacgactaa ttcgctgcca cgatcatagt cttctttata cccacgtttt gctgtaatat
    22861 ccatcagtaa ttccggtgcc aatttttctt cttgctcgat tgataaacca taacgatcgc
    22921 tgattcttcc tgaataatat cgggcaaaaa agtctaatcc gtaaatcgct aaatcatcta
    22981 aatggacaag ctggtctttg atcgcgcctg tcagcgctag ttttttgccg atttcttcat
    23041 cttcaaattt gggccataaa atccctggtg tgtctaacaa ttccaactca ttacctaaac
    23101 gaagccactg ctgacctttt gttacgccag gcttatttcc tgtctgtgca attttcttgc
    23161 ctactagtcg attcatcaac gtagatttcc caacatttgg aatacctaag accattgccc
    23221 gaatagcgcg aggcttgatc ccccgagcag catctcttgc ccgtttttct ttcaatgctt
    23281 ctttagccgc aggtactaga cctttaattc ctttactttc ttgtgccgtg atagctaatg
    23341 caagatggcc ttgtttacga aaatattctt gccacagctt tgtctgttgt ggatctgcca
    23401 gatccgcttt attcaataaa acaaggcgag gtttttgctg aatgatctga tctagcattg
    23461 gattacgaga agataaaggt aatcgtgcgt ctaccaattc aaatacgatg tccacatatt
    23521 tcattttttc agaaacttct cgtcgggctt ttgccatatg tcccggaaac cattggatgg
    23581 tcattgttct ctccattcct ttcataccgg aatattcccc ggctttactt gtttttttct
    23641 ttttcaatat aaattcactt ttttattata aagaaattcc atctattagt taagtgaaag
    23701 tgaaccagtc ctcattctat cacatcttca cttttttgat gatgcaaatt tttattttgg
    23761 tttctatata tgaatctaaa tttttgacag cgtttcctaa ataagagatg atagacgaac
    23821 aaaacagaaa ggagacgaaa caaatgaata gtatggtttt tgttaatttt cctgttgctg
    23881 atgtcaatcg ttccgttgct ttttatgaaa agctgggttt taaaaaaaat gaagaatttt
    23941 ctactgaaga agctggttca atggtatggg atgacacttt ttggataatg cttctctctc
    24001 atgattttta taaaaaattt ataaaagata aagaaatagc tgataataaa aaaacaagtg
    24061 gtgctttgat ttcgtttagt gttgaaaatg cggatgaagt aaaaaaattt gctcaaattg
    24121 caaaagaaaa tggtggtact ttccacgaag tagatatggg aatgcctgaa gaagaaatgt
    24181 tttcattaga agtccaagat ctagacggaa atacattaga acccgtatgg atgaagatgt
    24241 aagcagataa ctaatctgac gttttttata acaaaaaaac aaaggcggat gatacgataa
    24301 gtattctccg cctctatttt ttttattcgt acaatctctt ttttacagtt tataaaagca
    24361 gtcttctcac ccttttttta attgttagat gccagtgtat gtaaatcacc aatgtgtcat
    24421 gacacattgg tgattttatg gtatcctttt attattaact gacaaaggag agaatatttt
    24481 gaacactttg attgcaaaag acgtttttag attgcccgaa atgaatgcaa gattgttgtc
    24541 gacctctgct cttttattag cccttgatgt catcttatac aagatagcga ttggtcctgc
    24601 ttattttcaa atcacattcg gatttttaag tatggcgctt atgggttatc tatatggacc
    24661 gatctgggct ggtttgcttg aaatgttgtc gaacacttta aatttcacga tatttggttc
    24721 aggaacgttt caagctgctt ttcttatcac ggctttcctt ggtggattta ttaatggctt
    24781 atttttatat cataaaaaga taaacctgcc ttccgtcatc gctgcccaat tattgattat
    24841 gatcccagtc agccttttta tcaattcttg gctgatgtct gttttgtttg gtgcagattt
    24901 caaagtaatt tttctaggta gattcatcag gaatatgatc caaatcccta ttcaaataat
    24961 ggttgtttat tcatttatca aaactttaga ggctagaaaa gtgtttaaac gagctgtttg
    25021 attttacgta ctaaggtagt aaactgattt tggattaaac aataaagcgt gcagtctcaa
    25081 cagactgcac gctttattgt ttctcactgg atccatttta tttcatcaag tggataatag
    25141 acaaatcttg ctttacctag aatatcagtt ttattgatcg tgccaaacga acgactatct
    25201 tttgaaactc ttcgattatc tccaagtaca aaataattat cttccggaag cttggattgt
    25261 cccgttagct cttgtagtgt aaagtccgtc gtataggatg catttgcatg atctgatttc
    25321 aggtttttag ttagataagg ttccttaact actttcccat tgatcttcaa ttgatcattt
    25381 tggtagctga cattttctcc tggaagaccg attactcgtt tgatataaat cgtgccatct
    25441 gctaattgaa aaacaacaat atcaaatcgt ctgatctccg aaaatttttc catcacaacc
    25501 atatcacctt gcttcaacac gttttccatt gaattgcctt ccactggtac cgggatgaat
    25561 aaaaatcccc gcaacatgaa cgcaaggaat acagaaacaa gtaagtattt gaatacaagc
    25621 cagaagcgat taatatagcg ttgttttttt gtcaatctct atcaatccat ccctcttttt
    25681 ttcattcttt ctttattata agaaaaatat caaaaaaatg cgactagata gctgaaataa
    25741 aaataattct ttcaattagg ctattggaca aaaaactttt ttgatcataa agtacaaaac
    25801 tatctcttat atgtcatgat tattttctaa atatttttcc catgcttcgt caaagatatc
    25861 catggacggc aaatagtcga cattcagttc cagataatta gaaatttctt cgtagctaga
    25921 gctgtgctta ggaaattgga tgtctttacc ggcttctgta gcgaaaacag ataaagcatc
    25981 atgtgtgcca ccgcctcgca gtgtcattaa ataatgaaaa aagcttcgat tcacccttcg
    26041 ttcctccaat tttcttcgat aaaggcagcc cggcgtgcat ggtttctttt aaaattttca
    26101 ggatgctttt tataatactc ttgatggtct tcttctgcta gataaaatgg ttgggctggt
    26161 tcgatttttg taacgattgg ctgttcgaaa cgtccacttt cagctaactg tttcttactt
    26221 ttttcagcaa tgatccgctg ttcttctgtc aaataaaaga tcaccgggcg ataattgtca
    26281 ccgcgatcct cgaattgtcc gaaagcgtca gttggatcag tttgctgcca gtaaagttct
    26341 actaattgtt catatgaaat caatgttgga tcatagataa tctccactgc ttctgtatgt
    26401 ccagtcgttt ggctttttac ttgttcataa gtcggatctg gtacatgccc tccagtatat
    26461 cctgacatca ctgtatggat accaggaagc gtgtcgaaag gctggatcat acaccagaag
    26521 cagcctcctg caaatattgc tcgttccatt atgattcccc ctctaatact actcatccga
    26581 tgatttttta ttttcttttg tcttgctttc agtactgctg gtgctagttt tgttggtatt
    26641 tgagttgttt gaagcatcag aggtatcttt tggcaaatat agactaaacc gaatgtcatc
    26701 atttactaag tcgattttat ctgctttaat atacatcccg ttttgcattc ggaattgatc
    26761 caacctaagc atgatagtca gatcatctgt attgacttcg atccaatctg gtaatttata
    26821 gtttctcttg atcatattca tcacatcttt gattggcaaa ctcaatgttc cgatagaaag
    26881 acttctagct tttagttgga catttccgtt ttccatcaca tacggatcaa aatataaata
    26941 gaaatcgata ggaaaaccta gtacttcgaa agtaccattg agtagtgctt cattttccaa
    27001 ataaaattta tattcaatat cactatcttt ttgatactca ctaaggaaaa agctgatgat
    27061 ttgattgact ttttctttat ttgaattgat cgttaagaca ggatctcctt gtctttccgt
    27121 aagagctggt atttctggga gttctggttc ccgattagca aaaatacggc tgcctaaaaa
    27181 tacggtcgaa ccaagtacta ccccaagaag gataaggaag gcgaatttcc agtaatttgt
    27241 tttctttgtt ttgtttgaag atacgtcaga tcttgttttc atgtttattg atcatcctcc
    27301 ttctctaacc attggttttg tgtctcgatc agctgatctc tcactgcatt tgccatcaat
    27361 tgataaccta aattatttgg atggaaatga tcttcttcat agagcacatt atttttgatg
    27421 ccatttagat tttctgtcgt gctagttgtt tgatctgttc cttcttctgt gacacctact
    27481 tcctgattca atccttgata aagcaaatca ttgattggga taaagtagca gttcggcatt
    27541 ttttttgtca ccgtttctgt tcctttgttc caattgttaa caattgtctg catgtcagtg
    27601 atctcaggaa agttcaaata aaatggattg tagatgccta aaatgtaaat aggtgcggtt
    27661 ttattgaggg atcgaaattc ttccaatagt tctgtgacat tttcctgata gtttttcagt
    27721 ggctttttaa aggaattgac cgttaacccg aataaattat tttggataac tttcatcaaa
    27781 tcattaccgc caacagtcag tgtgataaaa tcagctgttt tgatattgtt tcgaatttct
    27841 tcttttgttt ttactcgctt taagatctgg tcacttcgtt caccatttac accatagttt
    27901 tctgtttcaa ccgcggtcaa attataacgg tcttttagat catttgcgac aaggggaacg
    27961 aatcctcctt gcttggtttg atctcctact ccttcagtca gcgaatctcc gatagctatg
    28021 tagtgaagtt tttctttttt atttggatct tctctcacag tagttactgg tttcattagt
    28081 gggccagctt tcggtataga agcagctaaa cctgcataga tgatgatcgc aattaataat
    28141 gggaacaaaa cgaggatcaa atgtcgtttc gttaatttca ttttttcacc tcttgtattc
    28201 gattggttct aatgttatca aaaattttat caaaaaggta gtctttatga aaaaagaaaa
    28261 caattatttt cctaatttta taaagaaaaa gagaataagc tgcaagcctt tcaggctgag
    28321 ggcttatcct cttttcgact aatctacata atacatgatt gcaaacgcgt ctttaccagt
    28381 atgcgtagcg ataaccggat tcgtatgcag tacagggata tgcatgtccg ggaataattc
    28441 ttgcaaaccc tctttgaatt cttgcgccaa ttcttttgca tccgcatgtg aaataccgat
    28501 ttgtcgcaca tttggcaatt ctttcagttc atttttcaaa ttattaaacc atttatcgaa
    28561 cgttttcttt cctcgcccct tagttacagg gattaattca gaatgggcga aattcatcac
    28621 cacgcgtatg ttcaaaacac tagacagcaa accagttgtt cggctgattc ttccaccttt
    28681 taccaagtta tctagtgtgg atattccgat gtataatttt gtattgtctt gtactttttt
    28741 gacttcttca aggattttct ctactgtttt accttctttt gctaattcag ctgctcgaat
    28801 tacctgaaaa gataatcctt ggtcagtggt atcactgtcg ataaccgtta ctttcgtttt
    28861 tgttagatta ctggcttgtc ttgctgcttc tactgttcca ctcaagcctt ttgtcatatg
    28921 gatcgaaatc acttcactgc cgtcttctcc tagctcatca tacatcgaga tgaattctcc
    28981 aattggtggt tggcttgttt tgggcagatt ttttgctttc gccatcattt ccataaatct
    29041 ttcgccgtct agttggtcat catcgggata tactgttccg tcgatcatga tggatagcgg
    29101 catgacacga atgcctaaag catcgcgtac atttttttcc atcgtacatg aagaatctgt
    29161 tacgattttt acatttgtca ttattttctc tctctctttc tttaacaaga tgccttattc
    29221 atgttaaact acatgtaaga caatttctat ttttagtata acagacaaat aatgattttt
    29281 cattaattac acctagtaaa ggagccgttt tcatggaaaa aacgaaattt aatcgaacgt
    29341 atgaaatatt aaatgaagtg tttaatgcag tcacccatgg aattgggact ggattgagta
    29401 tcgcgggact agtggttctt ttagtaaaag gtgcccactt gcattcctcc ctccatattg
    29461 tctcttatgc tatctatgga tcgatgatga ttcttttatt tttaacgtct actttgtttc
    29521 attcattgat ttttacaaaa gcaaaaaagg tgttccaagt attcgatcat agttcgattt
    29581 ttcttttgat tgcaggaagt tatactcctt tttgtttgct gagtatccga ggatggctag
    29641 gttggacttt atttgtatta atctggttgt ttgcgattct tggcattata tataaagcgt
    29701 tgacgttgca taaacgggag agagtatcaa aagtatctac gatcatctat atttttatgg
    29761 ggtggctctg tgtcgtggct gctgttcctc tttatcactc tcttggaatg actggtgtta
    29821 ttttgatggt agcaggcggg ctttcttaca cgataggcgc attcttttat agcttgaatt
    29881 ctatccgcta tatgcatgtt gtttggcatt tgtttgttat gttaggtgca ggttttatgt
    29941 acttctctgt tttattcttt acttaataaa aaaaataaag ctatggatgg gacagctcta
    30001 aataacagct agtctatcca tagctttttt atatcgtcaa ttcgcctgtc ccgcttttta
    30061 caggattctc attacgctga tacgtttcaa attgatgcgg ataacgattt ttttcatcca
    30121 tttctccttg actagtattg atcagtgacc aatcagacca gtcaacaggt ggaaaataag
    30181 catctccatc gaatacatga tggatgactg tacggtataa tatcgtacaa aatggcagaa
    30241 attcttgata aacagctgac cctccagcga tgaaaaagat accgtctgtt tctttttcca
    30301 gagcaagcac gtcttccaca ctatgcatga catgcacgcc ttctgcttta taggaagaat
    30361 ctctcgtcaa aacaatcgtc tgacgattcg gcaacggacg gctacccatc ccttcgaatg
    30421 tttttcgtcc catcactagt gtatttgctt ctgtcatttg tttgaagaat ttcagatcat
    30481 ttggcaaacg ccaaggaaga cggtcttctt ttccaatcaa tccgttttca tcctgtgccc
    30541 aaattgcagc taacattttc tcatcctttc tgattctaaa cagcgatagg agctttgatc
    30601 gccggatgtg ggtcatatcc ttctaattgg atatcttcca tctcaaaatc aaatactgac
    30661 tgcttttcct gattcaaaac taaagtcgga aatgcgcgta tttctcggct caattgttct
    30721 ttcatttgtt cgatatgatt gatataaaga tgagcatctc ctaatgtgtg aacaaattcg
    30781 ccaaccgcta aacctgtttc atgtgcaatt aggtgagtca gcaacgcata acttgcaatg
    30841 ttgaacggaa cgcctagaaa cacatcccca ctgcgttgat acaattggca gcttagccgt
    30901 ccctcttgaa cataaaattg aaacatcgta tgacaaggag gcaaagccat gctaggaaca
    30961 tcttccggat tccatgcaga aacgatcaat cttctagaat caggagtctt tttgattgct
    31021 tcgatcactt gtttgatttg atcgataaag ccaccgtctt ttgtttccca gtgacgccat
    31081 tgatagccgt agatgtgtcc aagatcgccg tatgtttcag caaaggaatc atcggttagg
    31141 atcttctcgc aaaaaagctt gctctgcttt ttatattctt cattgaattc aggatctgtc
    31201 aaacatctat ggccaaaatc agtcatatct ggtccattat agtctggact tttgatgtag
    31261 cgttcaaatg cccattcatc ccaaatatgg tttttatttt gtaatagata gcggatattc
    31321 gtatctcctt ttaaaaacca aagcaattcg cttttgatca atccaaaagg tactcgtttc
    31381 gttgttaaca aaggaaaccc tttttgcaga tcaaaccgca tttggtatcc gaataagctt
    31441 aatgtgcctg tgcctgttct gtcttcttta cgatgccctt cttccaacaa ttttcttcct
    31501 aagtcaagat acgcttgttc catccttttt cctcctaatc ttctacccat tcactcaagt
    31561 attcccaccg tgccattttt tcttcaagtt gtgcttctgt ttcagatacg tcgttttgca
    31621 attcttgtaa tcgagtgaaa ttatctcctt gatgattcat ctcttcttgg agcaatgaga
    31681 ttttttcttc aagttctgca atctcatctt caatcgtttc ccattctttt tgttccatgt
    31741 aagataattt cttcttttca gcaggttcct tgctctgtat ttttggtttc gctttttcag
    31801 caggctgctg ggtctctttt tgcttcagta aataactgga catgtttccg taataaagtt
    31861 cttgttttcc gtttccttgg aatactaata acttatctgc taccttgtcc aagaaataac
    31921 ggtcatggga aacagtgatc acagcacctg gaaaacttct aaaataatct tccaaaatcg
    31981 tcaatgttgc tatgtccaaa tcattggtcg gttcatccaa taataaaaca ttaggttgct
    32041 gaatcagcag tttcaacaaa aataaccggc gtttttctcc gcctgaaagt ttgcctatca
    32101 gtgtcccatg cataaatctt ggaaataaaa atcgttcaag taattctgct actccgattt
    32161 gactgccatc tgctgttttt gcttcttcag cagcttcttg taagtaagca atcatccgtt
    32221 tatctggtgc catttcttca ttttcttgtg tataatacgc aagatgaacc gtttcaccga
    32281 tagacattgt accgctatcc aggggaattc tgccagctag aatattaagc aatgtcgatt
    32341 ttcccgcacc atttttacca gtaataccta gtctttcccg agattgaatc aataaatcta
    32401 aatgttctag cagtgtttgg tcatttatac ggtaactgcc atctttgatt tccagtactt
    32461 ttttccctaa acgttgtgtc gctaggttca gctctaaatc atcttcttgg tttacttggt
    32521 acaagttctc ctttagatct tcgaaacggt tgatccgtgc ttgctgcttg gtgccacgtg
    32581 cctgtacgcc agccctcatc caagctaatt cctgtttata aagccgcttg cgcttttctt
    32641 cttgttctac ttctacacga tcacgttctg ctttttctaa gacgtaagct tcataatttc
    32701 ctttatactc gtataatttt ccaaatgaaa gttcgaagat tcggtttgct actctatcta
    32761 gaaaataacg atcgtgggta accatcaata gtgcgccacg gtattgtttc aaatagtttt
    32821 ccaaccactc gatcgcttca taatctaaat ggtttgttgg ttcgtctagt aatagtaaat
    32881 cgggttcatc aatcaacact tgcgccagac taactcgttt tttctgacca cctgataaag
    32941 tactgatttt tttatctaat tcactgattc ctaatttttg caaaataatc tttgcgttgg
    33001 tatctgtagt ccaagcatct tctttattca tttgttcttc tgctttagca taacgttttt
    33061 gcacatcagg gtcttctccg ttttccccta gttcgatcaa tgcttcctca tatgctcgaa
    33121 cagtttggat caaggggctg tttccttgaa agactgcttg gagaacagtt aattcatcgg
    33181 aaaattcagc tgcttgagaa agataaccaa tacggtaatc agttggataa aacactgttt
    33241 gccgatcacc gtcgctgcta tcgataccgg caataatatt taagagactt gttttacccg
    33301 tcccgttcgt cccaatcaac ccgatacggt ctttttcgtg gaccaaaaaa gaaatctggt
    33361 caaacaaatc tttttcacca taagtttttt tgaggtcagt tacttttaat tctttcaaca
    33421 tgttccctcc ttatcttgga tattgtatca taaaaatagt gaacttcagg aaaatttttt
    33481 tggtatttgt tctttttgtg ttctcaaaaa aagaacagga ccgattcgat cctgttctga
    33541 tatgttttgt tttttataaa acttgaaaat ttttaccttt ttattttatt ggactcgttt
    33601 tttttgactt tgctcgaaac gcaaatcgga aagaaaagtt atcagatggt ttgttcatcg
    33661 tataagattc taacaaattg atattcctgc gatagctgtt cgcagaaaac gtcgtgattt
    33721 tttcagcaac ttcgtattca tcaatgattt ttctttcaag ataataacct cttagcattt
    33781 ttttcgtaaa cagactatct tgctggatca tccacgaaga aatcaaattc cccgagttca
    33841 tttttattcc ttcaagtttg cgttgctgga taaaaaattg aaccatcgtt tcatcataaa
    33901 tatcagtcaa gttatctaaa ctttcaataa tcaactgagt atttttttga tatgtctctc
    33961 tgatttcttg gatatcttca tcactaataa atgtctgacg atcagcatat cttctttgcc
    34021 aaaacatttt cggatggaga acgattcgca ttaacctacg gacaacaatg aaccaaaagc
    34081 cgataaaaga caaaatctgg cttgtgatag aatgttcgaa tcgagaaaga aaacgggagt
    34141 aaaaacgata accattagaa gaaagcttgc ccgaacgata actttcatcc aatccatccc
    34201 gttcgatcga caaaatcagt gcttgtattt cttgtacttc ttgcttttca tcatcggtta
    34261 aatcttccaa gtacagatca cgtatccgtt cttgataatt ttctatcact gcttcagtgg
    34321 ctaaaaattc ttttgtatta cgatcaattt cctttatttc ttttcgcaaa acttcaacga
    34381 cctcttctaa aatcatcaaa gcattcaaat ccgtgctttt cgcttcctct gtttccgtca
    34441 gcataggcaa gacaatgatc cctatgacca aagtcactaa gatgacaccc gccgtcagaa
    34501 acaaaagcaa agaccgctgg taaaacatca tattattgat tgaaaatggc aaaataaaaa
    34561 tcgtagccaa actgactgtt ccctttacac cgccaaatgt taagatcaag atctcgttca
    34621 actgtttttt gaacttttta ctgccatctt tgaatacata aaaaagactg atagaaatga
    34681 atctgatcac aaaaagcatg acagagatta atacgataat cgctaataaa agaccgtttg
    34741 gatataaacc gtctccccat actggcgaga acacttgcgt caattcgatt cctaaaaata
    34801 taaatactaa tgcattcagt gtaaaagcaa tagttgtcca tgtactgtgg gacaagctcg
    34861 acaattctgc atcaaacaca gagattttcc taaaaccaga agcttggaga atacctgctg
    34921 ccactgcagc aatgattcca gatacatcaa atgcctctgc caatacatag gctagaaaag
    34981 gaagcaaaag ttcgatcagc agataccctg tgacatcttg tgctgatgct ttttcgatca
    35041 aatgaattat tttttgtttg aaccagacaa gcaaaaagcc gatcaaagct cccccaatac
    35101 tagaaactac tagactcatc cccgcgttta ctgctgaaaa agatccggtg atcagcgcac
    35161 caagagcaaa ctggaaggca gtaacaccag atgcatcatt caaaagacct tctccttcca
    35221 aaatatgcat cgccttttta gggatattca ctcgtccaga aagagaactg actgctaccg
    35281 catctgtcgg acctaacgct gctccaaaag caaagcaggc agcaagtggg acacttggca
    35341 acaggaatga taacgtcgca cctaccccga caagggtcaa aatgactcct ccaaatgcca
    35401 agaacaaaat tgtcccgaaa tttttgagaa tcgaagaaat atctgctttt tctccttctc
    35461 taaataacaa tggtgcaata atcataacta agaacaattc aggttcaaaa tcgattgatt
    35521 gtccccactc tgtcagacct aataccactc caaggaaaat ttggatcagt ggagctggaa
    35581 tcattggcaa aatgcggcta gctatgttcg ataaggtgat cgttactgca aatacaataa
    35641 ctaactcagc aaattccatc gtccatcccc tttatttttt attttgtgta agcattttta
    35701 aaaattgttc ttttttcttt aaaattttgg tttcgactac ttcaacctgt gtttggttcc
    35761 cctctaattc acactgtaca cgttttgact ccaaacgaag cagatcatgt ttcagacgtt
    35821 ccaactgacg atacatcaat atatttcttt tacgcaactt acaaagtatt tgacggcggt
    35881 catccatcga aagggcgcca tagcgatcat gcaacagttt tttttgttgt tcaaaactct
    35941 ctctcatttt ccagatctcc tatacacaaa cttccatcat tatagcaaag ttctttttgg
    36001 atgaaaaata tttcattgtt tcatcgttta ttgctagtta gaaatttcca taaatttttc
    36061 aacgcctcat tctattccaa atagtatcag atatcaagta aaacttaaga aagataaaag
    36121 tttctgacaa aaggatgttt actttcccat tcctgccttt cttgttcttt tatcacaaca
    36181 aaaaaatggg gctgcgacaa aaatcttgtc acagtccctt attctgaata aacggtgtgg
    36241 aagagacagc ctatcagtag taataagaca catctctttc acagcctcat ttttgcgtaa
    36301 aaatcgattt attcgaaaaa ttccccgtat acatcttcta cacgttgatc tggattgatc
    36361 acgatataca agttacctgt tttttcgctg atttttgttg attgatattg attgtctaat
    36421 ccttctgttc ctttatctag atatgggaag tacacaagat caggtgtgcc atttcggtaa
    36481 aggatttcca ttcgctcgat atcttcgtat ttcaatgcgc gattaaacat gcccatttcc
    36541 agtccgccta ggttgatatc atgtgtcgca actgcgtccc cttcatggaa gatttcgatt
    36601 ttaaatccag cacacggatg gatttcaaca aattcactgc cattgattcg tccaaaactt
    36661 gttgtgattt gtttgatcca aagatcgccg atatgacggc ggttgatcgt ccaggtttct
    36721 ccatttctaa aataaaattt tagcgctgtc atttctttac tcattaaaaa gactccttta
    36781 tgatgtaatt tattcttgta ctacctcagt ttagcacaaa tttatgtaag tggtcacatc
    36841 ttatgttttt tcttctaatg acgtatctgc ccactttaag tagccaacaa agctgagcag
    36901 atacagtcta gtgtttttta aatcaatttt tctttcgata aaaaatccaa aacctgtttt
    36961 ttatcatttt ttattctttg ttctacgacg gctttttcag ctaattgcaa agcttcacta
    37021 acccacttac caggtttttt tccagtttct tctagcaaga ttttaccatc tactgctaat
    37081 tcgcttaatg acttgatagg taaacgatca taggattctc ctacggcatc catctgaggt
    37141 gtttcgtcaa aatagatcat caattcttct acttgcagtg catgtttttt gcctaagaag
    37201 tacaattcga atggttccaa agctccagtt tctcgttgtc gtagtccttg gacaagtgct
    37261 tgaacgtctt taatcatttg gttagaacac ttccacgatt tcaaaaagga acgaatctca
    37321 ttttctttta gatccagttg gctgatcaat aatgcccatg cttggctctc ttctttcaat
    37381 gggacggatg gtaattcagc aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga
    37441 cagcaaatgt agcactctgt ttcaataaat gcatggattc ccgcggcccg ttcatttccc
    37501 aagagcatct tgatgaattc cacatttata cgttctacag atattttacc taatagctcg
    37561 tggttttctt tgatggcgta gaaggtttct tcctctaatt tgaaccctag ttgactgaca
    37621 aaacgcaacc cgcgcatcat tcgtaaggca tcttcatgga accgctcatg aggattgcct
    37681 acagctcgca agatctgttc ttctagatct tgcagaccat cgaacaaatc gatgatttct
    37741 ccatcttctt tcaaagcaaa cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc
    37801 aacgaacgaa cgaattcgac atgatctggt ctacggtagt cctgataagt tgattccgtc
    37861 cgaaatgtgg tgatctcata ttgttcatca ttttctaata ccaacacagt tccatgttcg
    37921 atcccaatat ctatcgtttt tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt
    37981 gcgatatcca catcatggat tggctggttc agcaagatat cacgaacact tccacctaca
    38041 aaataagctt catatcctgc attctctaat tttttcagaa ctggaattgc cttttggtat
    38101 tccagaggta attgttttaa tttcattttt ccaacctttc ctgcgtgttt cttgtcctga
    38161 gataaagact cagttgcatg gtgactgttt gacaagtccg tttcagagtc ttcaaggcca
    38221 ggaccacttc atcttgttta ctcgtcctta cgctcaaagc gattatggtc tctttgattg
    38281 aatttttcca tgtttttttc aaaaacttca gttaagtcga tattcaatga attagccatg
    38341 atcatcgtaa cgaataaaac atcaccaagt tcttctgcta ccgagttaac tggttcagaa
    38401 gcctttttgc ttttttctcc ataagtgtgg ttgacttctc ttgccaattc acctacttct
    38461 tcagttaatc gagccatctg agctaatgga gagaaatacc ccaccttgaa ttgctggata
    38521 tattcatcca cttcctgctg catactgtat aaagaacgct ttttatccat ctttttccct
    38581 ccaaaactat cttaccaaaa ctttaacgca aatcctagat aagagcaatt gagaaaatcg
    38641 aatttccatg ctacatttag gaaggaattt ctaaatggaa attctgtgaa aatacataat
    38701 tgaaagctgg aattcatatg gtcaaacgaa aaacagtaaa agaagttctt tttatcatta
    38761 taggaacaag tatttatgca tttggtcttg tttacttgaa catcgctaat catttagcag
    38821 aagggggagt ctcagggatc accttgatct taagagcttt atttggtatt gatccagctt
    38881 atacgacttt attaatcaac atcccattga ttttactagg aggaaagatc ctcggtaaac
    38941 gttccttggc ctatactgtt ctaggtacac tttctttgtc attcttttta tggatctggc
    39001 aaagggttcc actagaaatc aatttacagc atgatctact gatcgtttct ctacttgcgg
    39061 gtttagttgc aggtgtagga agcgggatcg tctaccgcat gggtggaaca acaggcggca
    39121 gtgatattat cgctcggatt ttagaacaaa attacggcat cactatgggg cgttccctat
    39181 tggcatttga tatcatcgtc ttacttgctt cattaaccta tatcgacttg aaacgtatga
    39241 tgtatacgtt gattgtttcg tatgttttca gtcgtgtgat tgattcgatc ttagatggcg
    39301 gatattctgc aaaaggtcta cttgtcgtct ccaacaaaag cgaagagatt gcaccactac
    39361 tgatgaccgg tctgcaaaga ggtgtgactt tcctttctgg agaaggcgca ttctctggta
    39421 attcaaaaaa aatcatctat atggtcgtta gctctagtga attaactgaa gctaagagga
    39481 tcgtccacga gattgacgaa aaagctttcc tttcaatcat caatgtgcat gaagtggaag
    39541 ggcaaggctt tacttatttg aagccacaga caagattact gaaaaaacta taagaaaaaa
    39601 gacaaagata ggctatgagg aaagattcca catccagctg acctttgtct tttttagtac
    39661 tatactattt ttaactaatt gttatgttca cttacccggt tacttttctt ttgtgtctgc
    39721 aaaaggatct ttcggttaac ttcatcagat aatttcaaaa aagcatccct atctccatct
    39781 tctagtgctt gatcgatttg agcataaagc atttttagtt ttgcctcttc ttctttttct
    39841 tgaaaatatc gttcaattaa ttcagtatct tcttcactga ctgaagaatc ccaagaagaa
    39901 aatggattgt cttctaagat agatagatat tctcttgtct gccaagcatt ttcgaatata
    39961 cattccacat ataaaggctt tttccaattc aaacggattt catgaaaaat ctggtctgtg
    40021 tccgtaaatt cttttccctg caaaaacaat ttcatgggtt ccccttcaac agatagatca
    40081 ctgatctgca gtcctcgatc cgcaaaattc gccccttcaa caaagtggac attgcttaaa
    40141 atcgcttcat gattggcaag ataattcaag atccaaaaaa cttctcttcg gctaaaagat
    40201 acatgattga ccatccacgt taaaaaattc ttcttttccc gtacatcaat tagcattttt
    40261 ctcacctctc tgttaggtca tctaataatg acagtgcctc catatctcct ggttcaagtg
    40321 ttaaataata agtcaataat tcttgcgctc ttttcagctg cccttcttct cttaagaaaa
    40381 aggcatactc tttcaagaaa tctggttcat gtgatagttc atgataagcc tgttcatagt
    40441 gtacagctgc aacggtaaag tcttctaatt cattataagc atgggctagg ttccattctg
    40501 cataaggatt agcagtttct tccatctgat tgatcacttg tatcacatct tcatatcttt
    40561 catcatccaa gtaaagatta cttaaagtaa gtaatgtttc atcttgtttg tctcctactt
    40621 ctaaagcttt cagtaaaagt tcttctgctc gctgtctatc atgcagacgg aaagcatttt
    40681 ccgaggccag gtgataaagt tcgacttggt aaggattttc tttgattcct tcttctaaca
    40741 ctgtttgtgc ttcttcaatc atttcttctt cttgaagagc ttgtcctaga tatagatata
    40801 acgattgata atgcggatta acttctcgaa gttcttccag atagttgatt gcctgctgat
    40861 tttctttcaa ttgaagattc gtaaaagcta gctggaacag tcgatcatct gtcctttctt
    40921 ctttcagtgc ttcttctaaa ggtacgatag ctgcttcgaa ttctccttgc atacttaagg
    40981 attgtcctaa acgttcctgc atcgagattc ctgaaatttc atttatcccg cttgccaaca
    41041 atgtttcata aaccactgct gcctctttga aacgatcaac tgaaaaatac aattcagcta
    41101 gcgcaaactg gatcaatggt tcatcaggaa gcaacccagc tgcttctttc aattttgctt
    41161 cactcacttc agggattccg ataacttggt acaaatcagc tatcgccaat aagctttgcg
    41221 gatagtaatc actttgctta tccacttttt ccaggtattc aaaagctaag tcgatctcat
    41281 tatcttcgat tgcaatttct gctaacggga tgttcagtcc gtcattttct gggtattggt
    41341 tgatcagttg ttcaaaaatc tgttttgctt cgatcaaaaa gccaatagcc agcaattctt
    41401 cgcctagttc agctaatata tctgctgggt cttcttttaa tgctttttct aaaaagattt
    41461 gtgcttgtga caagtcttca tctgataaag cttgcaacat tttttcgcta tttgtattca
    41521 tttcaattcc ctcttatctc actgacgatt tgttgaaata ccgtcattgt tctcaataat
    41581 tctttttcgt ttggatgttg gtatccgtct attataccta ttttttctaa aacaagtatt
    41641 tttttgcttt tttctgccaa aaaaagaact ttttccaaat actccatctt tgaaatctgt
    41701 tccagccagt ggattggata gccaagcaag tccagccaca caaaaaagtt ttttagttgg
    41761 aaatcaaaag gatatgcttg gagattccac acgaaatgaa acatcattcc taaaaatttt
    41821 ttcatactag cagaaagtag atggccattt tcggtcagat aaaaagcttg ttcaaacagt
    41881 ttaccataat ttgacaggtt ttctgcctct aattgataaa aatagatcat ttcttcgatc
    41941 atcccggcaa acggccgagt cgttaattgc tttttatttg gatagttttt atacagattt
    42001 tgcaaaaaac gatgatcaca aatcattccg caacagataa aagtcaaaaa gtcaatctgc
    42061 tttccgtcca cctgccgctc gctgattgtt tgatcataaa cgatgcgttc tggcagattg
    42121 atggtttgca gcactacacg atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct
    42181 cggcttagag aacggatcga tacagggatc atccatagtg atgaatcaag ggttgtatgt
    42241 ttttgaaaaa aacctgccag atccatgatc ccttcatttc caaatccgat gataaacatg
    42301 gattgatttt cgggatagcg tttgacgaaa tcaagcaggt tctttaattc tgttaaatga
    42361 ttgcattgag tgttggcaca aatataccag tcattgtttg cttgatttgc aaataacggg
    42421 ttgatcttat ctgcaaacaa atcataatag cgctgattcg ttaaaaataa gattggctga
    42481 tcaatggaaa gttctcttag ctgactgact agcgtttctc cgtaaaacac tttcgtcaac
    42541 gcatcttcta cttgataatc aagttccata cgcatccctc ctcttcttat ttttatttta
    42601 ccattagtaa gcaaattcta gaaaagataa gagcaaagct cacctcataa cttgcaaata
    42661 aataaaaatt aaataaaata catgtgtttt ctatgtatga catacggatt cggaatctct
    42721 gtctcttatt tattcctttt ctgctaggaa ataaaaagaa aagcacttgt agccctagag
    42781 ccacaagcgc tttttcaact gtcttattta acagcgtctt ttaatgcttt acctggtttg
    42841 aatgcaggta ctttgctagc tggaatttcg atttcttcac cagtttgtgg gttacgacct
    42901 ttgcgagctg cgcgttcgcg aacttcaaag ttaccaaaac cgattaattg aactttttcg
    42961 ccgttagcta aagcatcttg aatagttgaa aatacagcat ctactgctgc agttgcatct
    43021 tttttagtta aatcagtagc tgaagcaact ttttcgatta attctgcttt gtttgccatg
    43081 gatagattca cctcctccac aagagatatg atagaaatat catttagtta aaactttctg
    43141 agtggtccag aagtttaaaa agaaatattg attaacttca atattctgtt atcacgatat
    43201 catatgaacc gcttaatagc aaggttttta ccaattttca catgcttttt ttagtcatag
    43261 aaacgtaaca aacgaaactg tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg
    43321 cctattttct cctacgaggg ataattcgga tggacgtccc ttcaaaagta aatgcttttc
    43381 tgatttgatt ttctaagaaa cgttcataag aaaaatgcat caattcctct tcattgacaa
    43441 aaacgacaaa tgtaggtggt ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt
    43501 ttcctttatc tgttggtgtt ggattgattg ctacagcatc cataatgata tcattcaaca
    43561 cagccgaaga gatacgaaga ttctggttca tacttacttg ttcgatcatt tcaggcagac
    43621 gttccaaccg ttgtttagtc actgcagaaa caaaaacgat tggagcgtaa tcaagatacc
    43681 ggaattcatc acgaatctct tgttcgaaat cacgcattgt attggtttct ttctttacaa
    43741 gatcccattt attcacgacg ataataattc cacgaccagc ttcatgggcg taacccgcta
    43801 ctcgcttgtc ttgttcccga atcccttctt cagcattcag taccatcaaa acgatatcag
    43861 aacggtcaat ggcacgcatt gcacgcatca cgctgtattt ttcagtattt tcgtatactt
    43921 ttccgcgttt acgcatacca gctgtatcta tcattaagaa tttctgacca ctttcagaaa
    43981 cgaagtgggt gtcgattgcg tctcgggtcg ttccttcaat atccgaaaca attacacgtt
    44041 cttctcctaa aatcgcattg atcaaagagg atttaccaac atttgggcga ccaatcaaac
    44101 taaacttaat cgtgtcgtcg tcctcttctt cagtctcttc agagaaatac ttcactgctt
    44161 catctaaaat atcaccaatc cctagtccat ggcttcctga aacaggataa ggatcgccta
    44221 gccctaagga ataaaattca tagatatcgt ttctcatttc tggattatct actttattga
    44281 ctgctaaaat cactggttta ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt
    44341 ctgtcacgcc ttcgcgtccg ctgactacac aaatgattac atctgcttct tcaatcgcga
    44401 tttctgcttg atgcttgatc tgatccataa aaggttcatc gccaagatca attccgcctg
    44461 tgtcgatcac gctgaattct cgacctaacc attcaccttt tgcataaata cgatcacgag
    44521 ttactccagg agtatcttcg acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg
    44581 tagacttacc gacgttcggg cggccaacga ttgcaattgt tggatttgcc atattatttc
    44641 ctcctcacaa gtttccaatc tcacttagtt taacgaatgc attgccaact ttcaagcata
    44701 gattgtcaat taaacgaagt atttttattc cttattgaaa tactaatcgc ttttcaaaag
    44761 aaaaactgag acaaaagcag ctgctttcat ctcagtcaca gatacaaaat caattatttt
    44821 tcagaatcat cggttgcttt ttcttctact tcttctttca acgcatcacc taaaatgtct
    44881 cccatagtaa atccagtatt ttcttctggt aattcataag actcaacatt ttttggttct
    44941 tcttctgaag ctggtttttc ttctaaagct ttgatactca atgcaatacg atgttcttct
    45001 ggatggatct ctaatacttt cactttcact tcgtcacctt catgaagcac ttcatgtggt
    45061 gtcgcaatat gtttatgtga aatttgagag atatggacta gcccttctac tccagggaac
    45121 acttcaacaa aggcaccgaa gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca
    45181 actggagctt ttgtatcgat ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag
    45241 gaaacacgtt cttgttcagg attgatagac aacactttga catttacatc atcattgaca
    45301 gccaatacat cacttggttt tgctacatga ctatgtgaaa tctctgagac atggactaga
    45361 ccgtcaattc cgcctaggtc gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca
    45421 ctgagaacat ctccctcatg gatagttgaa agaatttctt ttttcttttc ttctctttcg
    45481 ttttccacta ccgctttatg cgataagatc aaacgatttt ctgatggttc gatttcgatg
    45541 attttaaagc tcaatgtttt gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc
    45601 accattgatg cagggacaaa accacgtaca ccgacgtcaa cgactaaacc gcctttgaca
    45661 acatttgtaa caggtgcttc gatgaccttg ccctcttgga aatctctttc gatatcttcc
    45721 caaacttttt tagcatctaa acggcgtttt gaaagtagat agctgccgtt ttctttatct
    45781 ttgccgatcg tactgataac gactaaatct aatacatcac cgacttttac tacttcgctg
    45841 atatcttcta ccggcaaagt tgataattct tttgccggaa cgactccttc aactccagcc
    45901 ccttcgattc cgacaatcac ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca
    45961 tcgccaactt ttacttcttg tacgcttttc attgcgtctt ccattgactg gttgttctct
    46021 tgattttgat caaattctgt cataacactt gtcctcctaa ccaacattct gccgtaagta
    46081 cggtactgtt tttagtttaa ttgattattc aaaaaaaata aagcgatttc gttcgttttc
    46141 cttgaaaaaa caattgtttt tcattttttt tcttaattta aaaaagaaga taaccttttt
    46201 tcaaaacaac agcttttatt gctgcaacga cttctggaat cgatttgcca gtcgtatcga
    46261 tacgaacagc atcctctgct tgcttcagcg gagagacgtc ccttgttgaa tcataatggt
    46321 ctctttcctg tatctctttc gtcagtgttt caagatcagt cggaattcct ttttcttggt
    46381 tctctttgaa acgtctttct gcacgctcac ttacgcttgc tactaaaaag attttcactt
    46441 ctgcattcgg taatacagct gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt
    46501 cccctatttt tcgttggaca gcaactaatt cttctctgac ttttccatgt gcagagactt
    46561 cagaaacagc ttttgtgaca tctggttgac ggatttctaa agtcacatct tttgatgcta
    46621 tgaatacatg ctgtccatct tcttgttgcg aaaaagagat aggatacttt ttgatcagcg
    46681 aaactagccc ttcttcatct gaaaaagata cattgttcgt aatagctaaa taagttactg
    46741 cgcgatacat ggcaccggta tctgtataaa tataattcaa ttcttttgct aatatcttgg
    46801 caacggtact tttcccagat gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga
    46861 tataactcct ttttctcaga aaaaaagccg caaagcgcgg ctattattat ttaattctta
    46921 attcttgacc agggtaaagc ataaagttgt ttggatctaa gccattcaat tcaaacaatt
    46981 gatcagtggt gatccctgca cgctcagcca cttgttttgg accttcgcct gcttgaacag
    47041 tcgttgttga accggctgca gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg
    47101 aatcccctgc ttgataagtt gcctcacttg aggaagtcgg tgttgtctct gctgcaatac
    47161 tgctttctga cgttacacct gtatcttgag acacagtaga ttcttccgcg attgtactag
    47221 attctactgt tgaagtctca gttgactcct cttttgttgt acttgtagaa ctttctacta
    47281 ctgatgtcga agtttcagaa gaagttgctg ctgttgcatt gttgcttgat ccatttctga
    47341 tccagaaata agcacctgct ggaatagcga tacataagac cagtaaaatc aaaaatagcg
    47401 aaagaaataa tgtatttcct ttcttttgtt gtctctgaga acttcttgat gaaacttcat
    47461 catctgtatc gtagattggt tgttcccatg gttcttgtgt ttcgttattt tctgtagatt
    47521 gatgtctatc ttttctgctc actgttttac ctcctaaact ctatcgtgtg tattatacca
    47581 taggaaaaag tgtcttgtcc taaaaaatca atgttccatg ttttttttaa ataaatttag
    47641 taaaatcgtt ccccaatgaa gttgttcagt cttagaaaaa agcttcctct ttggcagaag
    47701 ttctgctttt gctccatcaa tatcacaaca attctgcggc ttttcggaga gtttttcgcc
    47761 aaaataagct aagatgaatt ttctgcggca gttttcttca ttaatatagc gaagcatctg
    47821 ctgcaggcgg aattcttttt ctttttcctg acgtttgagc cgttccaccc aattttctgg
    47881 tttttcagac tgctggatca gctccatcca ttttttttgc agctcatcaa agggagcttg
    47941 ctctgtggca tattccaaat aaagctcaaa actttgtcgc tgttctctag aaagttgatt
    48001 aaagaaataa tggatacgtt catccccact ttgataaaaa agaattgctg cactttcttc
    48061 ctgatctctg cctgctcgac cgatttcttg tacatagttt tctagactgt ctggcaaatc
    48121 ataatggacc acataacgga tatcagattt atcgatcccc ataccaaatg cattcgtagc
    48181 aatgagaaac tgcagctgat tttttacaaa ctgctgttga agttgtcttc tttgagctgc
    48241 atcaagacca ccgtgatagt atccaactgt aaaacgttcc ctgaataaat gatacaatcg
    48301 ttcgacttct ttttttgttg cacaataaat gattgccgct ccttgtgctc tttccatgaa
    48361 gtgttcaagt tcttgttctt tttgctgcgt tttccgaaca aaaagcgaga tattttgccg
    48421 attgactgat tgtctgatct cttttggtgg tcgatcaaat agtacatgac gtatatctct
    48481 tgctactaaa tctgttgctg tcgccgttag tgccagtgtg accgggtttc ctaaaagctt
    48541 tcggattttt cctaactgtt gatattctgg acggaaatca actccccatt gagacacgca
    48601 atgagcctca tctactacat ataacgcaat attttgtttt ttcagatgct ctaaaagaga
    48661 aggattagtc aacatttcgg gactcaaaaa caaaaattta tattgcgata gatgacggag
    48721 gacgtatctt ctttcagatt gcgaaagtaa actattgaaa gcagctactc gtttctcccc
    48781 tcgtttttgt aattgcgtca cttgatcttc cattagagaa agcagcggtg acacgatgat
    48841 gaccagacct tccattaaat accccgtcaa ttgatagcaa aggctttttc ccgttcctgt
    48901 aggcaaaata gcgagagtat cttctccatc aagcaacgcc tgaataattt cttcttggcc
    48961 gggacgaaat gaatcaaatc caaaatattt ttttaattca tttattaaca ttctgtatcg
    49021 cttcctttct caatagatag atttgataca aacgaaattc tccataatct aattgtccat
    49081 ctacattcca ttcgctaaag cgccactctc gcacggaatc cttatttggt tccaaacgtt
    49141 cgattgtttc tgcagagaga atccactcgt atggaaaatc atcaaaaaac aaggcccact
    49201 caataagatg atctgttact gttcctcttt ttaaatgacg gattgccatg atctggtcaa
    49261 ttgtctgacc attcataaac atctgcttgg tgatcatcat gctttgattg aaattttgct
    49321 gtaataatga atcgatcaaa gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta
    49381 acaaaaggtg gatacatctg ctttgttgca gataaaccgc tgactcatca ttgttttttg
    49441 ccagctgata aggaagcagc cctgttcgct catttccaga taattggtta gctaaaaaat
    49501 ctgctgcttc ttctggtatc tccgaaaaca gctgcaccaa actctcatac gcagtatcaa
    49561 tcaaaatttc acgtggcaat tggctttcac taagccattt ttttagttgg aaagtataaa
    49621 aaggacttgt ctctgctgga agatagtctt gaatacccgt tgccagatta gaaattactt
    49681 gaacagcaaa tttaatgagc cgccaactag tttcccctgt tctgccatag cggtcaaaac
    49741 gcaaaccagt atgttctatt tgatgttcgg acaacatatc agcccctgca ggcgttagct
    49801 ttgcttcatt ttcatttatt tcgatccaac cttgctgcgc aattttttgc aaaatttgat
    49861 aaaacttatc ctgcttcaat tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa
    49921 atccataaat aagtactgaa gtcgttcgtt ttcccacaag gagttggtaa agagaactaa
    49981 tccgcagctt gtgtcctgat gaaaatagcg ataaaataaa aacaatcata ttattctcct
    50041 taagttagga tggttagaaa tgtcactact atgtaaactt gtacctgaac gctgtattgc
    50101 atgcggtctt tgtcaaatcg aagcgccaga tattttcgat tatgatgaag aaggaatcgt
    50161 tttatttgcc caagaaccag aggctcatca acagttcgtg atgccggaag aagaaacata
    50221 tgtaaaaaaa gcgtatcaaa aatgtccagt tcgagcgatt ttattagaaa ataaaatata
    50281 aatataaacg aaactttaaa aacgtacact aagttctcta aacttgaaaa taagccggga
    50341 tctgtttctc ttgttccgtg aaatcccgac ttattttgaa agattcattt gctatctata
    50401 gttaattgac tagttttttc acggacccta taggacccat ttatattatt tcaacttttt
    50461 cagccagttt tccaatgtat tgacgatcac agtttgctgg tctgcgttag aaatttctgc
    50521 ctctccatct cctttttgct caccatagct gccaaaacct ccatgatttc cgccattgat
    50581 tgatacatac tctgttcttt ccggtaaata ctgcttagct tcttgataag cttcctgatt
    50641 caatacttta tcttttgtcg ccgtgatcga taaaacagga acagctgatt ctgtcaatgt
    50701 tccttttttg tcgggataac ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg
    50761 attcttattg acataacgac ttgccattac gccacctaat gaatggccac cgatcacata
    50821 atcggaattt ggaaaatctt ctttgacctt atccgctttg tttccgccca tcactgctag
    50881 atcaagtggc attttgacaa tagcgactga aaagccgttt tttgctaaat cttgcgccca
    50941 aatactataa ctttctggct ctacgagagc accaggataa aaaataagta atggagactt
    51001 tccatgattc tttttctctc cttcaaaaaa taagtaatct tctttgtttt ttccttcttt
    51061 taccaacact tgcgcttctg acgatggttc gtacgtattt tgctgtatat agtaataacc
    51121 ggctccaccg agtaatccta aaatgatgac taagccaatt agtattcgtc taaatatttt
    51181 cactttctcc caccttttct atctgagtga cccctttttc ttaatcaagt atgattatac
    51241 cttaaaaaaa taagagagaa atgcaaactt tccagcatca aataaaaaaa gacggaatat
    51301 ccatttgaat atccgccctt tttgtatgtt tatttttatt tagcacataa acgatacaca
    51361 gcttctgcat aaatttccat tgctttatac atactactta attcccatcg ttcattcgcc
    51421 tggtgcataa agtcaggagt atcagggaac atcgcaccga atgccacaca ctggttcatc
    51481 gtacgagcaa aagtagcgcc gcctgataca aacggttcag tcatgtctcc agtaatatcg
    51541 cgatatgtcc ccaatagatt tttcactaac tcactgtcta gtggcacgta taatgaatca
    51601 aggaagtcaa attcttcata agtcaaccca tatttttctg ctgttttagt taatttagct
    51661 actaaatcat ctttcttgaa tgtaacaggg attctcatat ctacaccgat ttttgtttgt
    51721 tctggtgtga tttccaaact tgcaaagttc atcgttagct cgccggattg ttcatcttct
    51781 gttttgccca cgacagcttc accagttgca ttttcttgaa ctaattttcc taagaaattg
    51841 attgggccaa aatcaaatac ttctgaaaga gcaattgcca gacgagaaat agcattcact
    51901 ccttgtgctg catctttcgc gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt
    51961 tcttcgaaat cgaattcgtg ttttttcaat gcttctttta cttcagaaag cttttctcca
    52021 gagtaaggag ctgcatcagg aacaacattc aacgctcctc cagcttttac agaaaactcg
    52081 ttcgttcctg gtcctgtcaa gtaagcttgc aataagcctt tttctgcata aattaaagga
    52141 aattcagcat ctggcgcaaa tccttgcgta attccttctt ctttttcgtt atatttttct
    52201 aaacaacgcc ataaattttc ttcatctgta ccaaagataa aacgaatacg tgtcttgaat
    52261 tccacgccag catcaatcaa agctttgacg gcatacattg ccgcaataga aggacctttg
    52321 tcatcttggg atccgcgacc aactaaccag ccgtctttta tagttggatc gaatggtttt
    52381 gtttcccaat tattttcgtc ccctgctgga acaacgtcca tatgacacaa gataccgaat
    52441 aattcatctc cagagccaat ctctgaataa ccgtaatagc cttctggatc tttaaatgtt
    52501 ctaaatccta gcttttcact gatttccaat actttatcca aagcttcaat cactttttta
    52561 ccgaagggat gtccttgtcc tgaatctgct tcatctagta cggaaggaat tcggatcaat
    52621 tcgctcaatg attctaatgc ttgttcatga tgttgttctg tcacaaattt tttcatgata
    52681 ttatcctcct aatatgttaa aaagggtcat gtcaaattcc tccgacacaa cctcttcttc
    52741 tcaaataatg acgttttaag cctgctccat cagcaagcag aatgttctta acaaatgatg
    52801 aaagaaaaaa cactcactga atggagctat tgacttatcc acgtgcttat cgatagccgt
    52861 ttatcggttt cttcattctt atgatagacc ttgtacctgt atttgtctag gtttagaaca
    52921 ttgctgctac acctaggatc acacagatgg ctacaaataa tatcgcaatt aatttaccgg
    52981 taaatttcca ccacactgaa acatcgaaac gagcaattgc tacagcaccc attacgatag
    53041 cagatgttgg tgtaattaag tttactaaac cagatgcaga ttgataagca gtgattacta
    53101 aatccttacc gacgccggca aattcaccca taggtcccat gattcccatt gttgcagcag
    53161 ctaagccgga agttgaagga atcaagaatg acatagggat atagaaaata aatgtaataa
    53221 tgatgaatac acttgaagat aatgaactta atccttgttc gccccaatgt aagatggtgt
    53281 ctgtgattaa tccattgttc ataaccactt ggataccacg agcaacagct actacgattg
    53341 ctacaccgat caaatcagaa gcaccagcta aaaattcaga gacaaattca gactctttca
    53401 tgccataagt gattgctact acgatggaca ttactaagaa taacatagta atttctggga
    53461 agtaccagtc acctaatgga agcatgcttt tacctagaac cgtacctagt accggaaggt
    53521 ttgttaacca gtttgtcaat gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag
    53581 gaatcaaact gatgatcatg ataccaaaag taataaagaa caatacatta acatgttttt
    53641 gttttttcgt gattacttct tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt
    53701 ctttacgttg gttgtatact aatgattttg ttggatcttt ttcgatcttt gatgcataac
    53761 gatacacata tacaatagaa atagctaaca aaataacgaa catcaagaat cgaagaccaa
    53821 taccttcacc catcgaaatc ccaacagctt gtgaagctac gcctgttgca aaaggattta
    53881 ccgttgaagc tagacagcct acttgagacc ctactaatac gattgctaca gctactactg
    53941 tatcaaagcc tacagccatc atgactggga tcagtaaagc atagaatgga atcgtttctt
    54001 ctgccattcc gtaagtagag ccgcctaatg caaataacag catcaatact ggaataagca
    54061 ttttttcttt tcctttgaat ttcttcacaa ctgaagcaat cccggcatct agtgcccctg
    54121 ttttgttgat aatacctaaa aatccaccaa caaccaaaat gaagaaagag attgggattg
    54181 cagcaggtgt ttctttagtt agctcccccg ctggtacacc tagcatccca ttgattggcg
    54241 ccatgaaaat atcccacagc ccttgaggat tttgttgtac tcgttcatat gatccagcga
    54301 cgatgttgcc tgcatcgtct acactatagt gcccagctgg tataaaccaa gtaagtacag
    54361 cgattactgc aataataata aataagaccg tatacgctga aggcatttga aattttttct
    54421 tttttgtttg ttccatcttc ttcttccttt cttttccgtt agagacaaca attttggctc
    54481 taatgttatt ttatcaaaaa gagtatcaga acaaaaataa taacacgcat tttgtaaaac
    54541 ttttgttcaa aaagttcgtt tttccctatt ccctcctctt ttttcaggtt tttaaagctt
    54601 tcttcttttt cattgtagcg tttccttttt tttataaaca aacattttcg tcttttaaaa
    54661 ggagaagaat ttcacatttg caaagaattg attgattttt atgcataaat caaaaaaata
    54721 ctcaaaacaa acaaataaaa cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa
    54781 aaagaccaag aaatttcttg atctttttga tatttatttg actgctgtat gacgttgttc
    54841 gaactgatgt tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac
    54901 agcactcacg atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt
    54961 tgctaaagac atgcccagca tctgtgttgc gttcagccct aaaaacatta agtaaagagg
    55021 cgtaataacg aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat
    55081 gacacctaaa aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat
    55141 cgggaatgta aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg
    55201 agaaaaacct gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc
    55261 cgctggaccg aataagaaca tgctgatcaa taccgagata tcactaaaat cgatcttcaa
    55321 aaaactaaaa gccggcatga tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc
    55381 aaacatcgct actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt
    55441 taggactcct cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc
    55501 cagggaaaat agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt
    55561 atccagacta tactgtcggt atcggaattt caccgattca gctatttttt tgcaaaatag
    55621 gtcgtggact ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg aaccttatat
    55681 aatttttgtt tacatattca ttatacatat ctattcaaaa aacgcaacat ctaacactta
    55741 taaaaagtaa gtgttcattt tgagtggttc agcaaactgc tgacttcttt tttgttcagt
    55801 tcacggtaat ccccaggacg taggccttgt aaagtcagat ctccgtattt ttcacgtttt
    55861 agtttttgca caggaagacc aactgcttga agcatatttt tcacttgatg attacgtcct
    55921 tcgtggatcg tcaattccac gatactgtgg tttgtcttag gatccactga taaaatttgg
    55981 aatttagctg gagatgtttt tcgtccttcg atctttattc cttttgtcaa aggtgcaagc
    56041 atcgattttg tagcgatccc ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt
    56101 ggatgtgtca ggcgctgagc aaaatctcca tcatttgtca gtagcaagat ccccgaagta
    56161 tcgtaatcca gtcgacctac aggatagata cgttctttta ccatcggcaa aagatccgta
    56221 acaacttttc ttcctttgtc atctgcaaca gcagaaataa ctcctttagg tttgtacaat
    56281 aaatagtaac cgtattcttc ttgataaatt gggacttcat ccacttccac agtgtcatgt
    56341 ttcccaactt ttatccctaa ttcccttaca acttgtccgt ttacttttac acgtcctgca
    56401 aggatgaatt cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat tactttctgc
    56461 aatctctcca ttacaattat tctcctttat tcattccatc aaagaataga tctggtgaca
    56521 cgtcttcttc aagtgaaatt tccatttctt gtgtatcagg cagttcttct aatgatttca
    56581 aaccaaaata atctaagaag tagccagttg ttccatataa tatcggccga cctggtcctt
    56641 ctactctacc tttttcttca atcagtttat gggcagctag acgctgtacc gctcctgaaa
    56701 cctgaactcc tcgaatctgc tcgatttcag ctcttgtgat tggctgttta taagcgatga
    56761 ttgctaaggt ttcgacggcg gcttgagata atgtgtttga tgtcccttgc gcatatcgct
    56821 taagtaaggg agccagctct tttttagtcg ttaatacata atggctttcc gtttcaaaaa
    56881 tatgcaaagc tgagtgcgag ttttgagtgt aagattcttt taattctttc actaaacttg
    56941 tgacttctgc ttcgctgaga tttaacagtc cgcttaattc ttcattactg atcccttcct
    57001 cgccaacaat aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc ggtatctcct
    57061 actgctggat aaagcataat tgattcatag ttttcttttt gttgcgcaat gatctgccct
    57121 tttttcatca attctagcaa agccataaaa gtagtgacaa cttcactttt agaaggtgac
    57181 tcaaaaaaag aatcgaaagt acagccatgc gcatttcttt ttatacgctc ttcgataaat
    57241 accattcgtt cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa
    57301 aatttcttac gttctaagat tttctgcatg gcacggatca aatcgatttt tttgacttgt
    57361 cctcgcttca acggcttttc agcatccgct aacccatcga tgttcatagg ttcttttgtg
    57421 taataaagac ttcgttcctc tgctttttct gtcagtcttt cagctgcata tttatattta
    57481 cggtattcta aaagctgagt gaccaattct tctcgaggat cttcttccca taattcttca
    57541 tcgtctgtga cgaattcttg gacaggaagc aatgtcttgc ttttgattgc catcaatgta
    57601 gcagccatca ctaagtattc tcctgcaaca gctagctcga gtgttttcat tgtatggata
    57661 taattcatat attgctccgt gatcgtagcg atcggtatat catatatatc gatttccatt
    57721 tgctgaatca gatgaagcag cagatcgaga ggaccctcga atacatctaa tttaatattg
    57781 atctccgtca actcattcac tctttcttct ttttctttgc tagccatttt ccaatgtaag
    57841 gctctgtgat ttcagttgca cggatggaga catccgggta cttttcctgt aattcatcta
    57901 acatttgaaa gcccatattc tcaccgcgat aagacggatt caaagaaatg tcatggatga
    57961 tcagctgatc gttcgttgcc cagtaaatac caataatccc ttgaatgttg gttgaacctt
    58021 ctggcgtaaa gaaaaaaagt tcataatcgt cttcagattc gtacgtatca atctccttca
    58081 acaaagaagt tttacctgtc agtttcttat ggaaactcaa tagtcccata gcaattttac
    58141 ggttttcttt ccggtaatgt gttaacatct tctcaccttc ctatgactaa gcccttggaa
    58201 aatgctgctt ataaacatcc gtcattcttt tcttcgtgat atgtgtataa atctgagtcg
    58261 tagagatatc cgcgtgacct aacaattcct gtactgtccg caaatcagct ccattttcta
    58321 gcagatgtgt ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg taaatcccag
    58381 cctctcgtac taactgcttc agatttttcc agattccttg ccgagaaaga cctttcccat
    58441 gattattgac aaatacatgc atttcagatg cgtcctttac aagtaatgga cgtgcttcgt
    58501 ctaagtagcg ttccagccat tggatcgcat aatcacctaa aggaatgatc cgctctttat
    58561 ctccttttcc aactgtctgt accagtccaa gagaaagatg aagatctccc aatttcaatc
    58621 caatcaattc actgacgcgc atcccagtag catacatgac ttccaagatt gcacgatctc
    58681 ttatccctaa cgtttttgtc gtatcaggtg cttcaatcag cttttctact tctgtcagag
    58741 tcaatgtact tggtaatttc tgtgctttct tcggcgtatc gatatgttgc atcggatcat
    58801 gatcagtgat acgctcttgc cgtaaaaact gatggaatct tctgagactt gagatcatcc
    58861 gagtaacagt tgctgtcgct tttttttctt catgcaactt ctccagaaaa cggatgacta
    58921 aaaagcgatc gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact
    58981 gttccaaatc acgttcatag ctttgtcgtg tattggcgga caatccccgt tcaatattca
    59041 agtagtggag ataatcgttg atttgctctt tcataataat ccccctcatg cctctctatc
    59101 atatcagata ccgcttcatt ctgatagtct ttttccataa tctgagcaaa gaaaaagccg
    59161 tctagacact cagacggact ggtttctttt ttctttttca tagcattcct gacagattcc
    59221 atggaacgtt agccgatgat cttttactaa aaaatgataa cgttcttcta ctacttgctc
    59281 cacttcaagt aaaagatcct cttctacttc ctcgatcgtg ccgcagttca agcatagtaa
    59341 atgatgatgg aaatgtttgg cgccttcttt tctcaaatca taacgggcta atccatcatt
    59401 aaaacttact ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc gataaacagt
    59461 agcaagtcca atttccggac tctttttttt cactaaaaag aagatttctt ctgcagataa
    59521 atgctctttc tcattttcca aaagcacaag cagagtagct tctctttgcg gtgtcagctt
    59581 aaaccccgct tcatgtagct gctgttttgt ttttttcatc gccgaaatag cattcatgac
    59641 catttactca accccaacaa tttataataa ttacaaataa aaaaagatat aacactatgt
    59701 taattaaaat tattataagc tagttaagaa aaacatgcaa gggattctca attagatttt
    59761 ttgactagtt ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa aagattacct
    59821 aatgcgcgct taaatgctcc tttgctgata ccaaaagctt tcatgatttc ttctggatcc
    59881 gatttatctg taaagttgat ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt
    59941 tgtgcatcat ctgatatagc ttcatatgct cttggcttca atgaaagatt cagtacaccg
    60001 tctggtcgga caccaatcac tcgacctttt atctgctcgc ctagacgagg ttcattatat
    60061 cgttcagaag gatggataaa cccaagatag aaatcttccg ttaaaacgta cgtacctgtt
    60121 aatttcaagc ggtatgctgt tcctatcaca tctttgtttt tcatttcttc tgttccatat
    60181 ttgcttaaag atttgaatat tttttcatct gctaaactgc cccaaatacg gtcttttgca
    60241 tcaactctaa gggaaatcat caagcgatcc ccttttttag gccatagctc tgtcattgtc
    60301 ggcaattcat caagagatac gacaaagtct ttatctggaa gaccgatatc gacaaacgca
    60361 cctaaatctt ttcgtgtagc agtaacgatt ccaaaagcgt aatgaccgat acggctttta
    60421 ggaatttgtg tagtgatccg attttcatgt ttttgatttt ggtaaccgaa tccttcaact
    60481 gcttcaccga ttttgtgttc accttctgat ttgcttaagc caaaggtttg tccgtttttt
    60541 tggacaaagt agtttttttc attttcatca ataataatac ctgtaaatat agttgctaat
    60601 aaatcgttca tttttttctc cctctatagt gaaaggtttt cttaatacat ttctttgcta
    60661 tcataacata atgttatcta cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg
    60721 agtatgacaa cagttgtgtc atactcccct ttttcgaata aacggtgtta aacggcaggc
    60781 tcacagcctc tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt
    60841 ttacagttga tggttacgat gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat
    60901 gcagattttg ttgaagtggc atatagatta taatagcctt ccccattttc gattctggct
    60961 ttttctaatc tgtaacctcc ttcaacgata tatccacgtt ggatacatgt agcaatcact
    61021 ttgctttgga cacctggtgc ccacgtccat gctggattag atgcatcggc aatatcactt
    61081 gctctggcag atttgattgg ctgattcgct gagtttgaag aggaaggggt cgaggatgcc
    61141 gcttcttgct gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc tgcagctgct
    61201 gcttgagcgg cttgttttgc ttgttcttca gctgcttttt tcgcttcttc tgcttttttc
    61261 ttttcctctt cttctttttt ctctttatca gtgagcatct tatctacttg tttcaattgt
    61321 tcattcaact gagcagatag atctgtattt ttcacttttt ctactgcttc ttttgccgtc
    61381 ttgtattggt cgcgtgttgc tgaatccact acttttccgt ctttaaagac tatagctgtt
    61441 ttttctttag ctgtttgcaa ttcagcatat tgatttttag cttctgtttg tgcttgttga
    61501 atcaattttc caaaatctgt gttatccgtt tcgattgctt ggatctcttt atctgctttc
    61561 aacttgatgt catctgctag atgatcatcg acgatggcag gtgctatgaa ccactcattc
    61621 actttttgga tatctcggat cttagattgg atatcttggt aagttgtttc taactctgaa
    61681 tatccttttt cattcttaag actggataaa tcatttttta gttttgataa atcttgatga
    61741 atcatatctg cacgaataaa ttgatggtga tcatctgtat aaaaggcagc aagtttctca
    61801 tgaagttctt ccatttgttg ctgtcttttt gcttctgctg ctacttcggc ttctactttt
    61861 tgatgatggt cataataagc ccagcttcct ccaccgagta gaagtactgc tgctaaaacg
    61921 agataaggta ctttttttga ttttttcttt tgcgctttat ctggatctgt tttttctaag
    61981 tttgattctt gatgtgtttt ttcagtatct ggcacgattg ttattgattt cgcttctgct
    62041 tcaaacagct taggctggtt ctcttctttt tctatttttg cttcaagatc agtaccctct
    62101 ttgcttacag attgattttc agcatcggat tcactattta tttcatcggg ctctgttgac
    62161 tttttgtctg tgttttcgag tatttctttt ttctctgctt ccggcagttt attcgttttt
    62221 tccttttcta attgtttttc agtaaatact tcagtttttt ctgtgatatc cgtttttacg
    62281 tcttttattt ttactgtttc tttacctgtg gcgttttctc tatcagagtc tttttcagtt
    62341 tcttgttcct tttctggctc tactttgttt tcctgtgcaa ttttttcact gatcattttg
    62401 agcagctctt tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat ataagcggat
    62461 agtatcgaat tttcttcctc attatcgacg atttctttca gttcagctac tcttcttttt
    62521 tgttttgatt caagcgtttc ttcttgattc tttcctgatt cttccgaagt ctcatgaaca
    62581 gttgctttct tctgttcatt tttctctata ttagttactt gatcgtgagc aggtggttct
    62641 tcgtcttctg gttctgcttt ttcgaataat tccattacag attctagagg taaatctttc
    62701 aattccgacc attcgattgg atcgtttaat tctgtatcag gaaagactgc atcttcagtt
    62761 gatttatcct gcatagctgc atctgtattt gtttcttcaa ttgattcttt tatttgctga
    62821 tcttctgcag ttatttgatg accgcagtct ggacagatag tattctgctc catttttttc
    62881 gatccgcaat ttggacattt tttcatcact tagcgatcgg ctcctttact aggtttacgc
    62941 taccatttaa tctatcatat tctatacgtc acgccaatag cttattttta ggatttactt
    63001 aaaaataatg aatgaatttc cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa
    63061 gctttttttc ttttaaaata aaccaaacag ttttactgat ttctcttctg gtcaatagtc
    63121 cacgcttatt ctaagaaatc ttttaattgt tttgaacgag aaggatggcg caattttctt
    63181 aatgctttag cctcgatctg acgaatacgt tcccgagtaa caccaaatac tttccctact
    63241 tcttccaatg ttctcgtccg gccatcatct aaaccaaaac gtaagcgaag gacattctct
    63301 tcccgatcag tcaatgtatc tagtacatct tccaattgtt cttttagtaa ctcataagct
    63361 gcgtgttctg ctggacttgt tgcttcctgg tcttctatga aatcacctaa gtgagagtca
    63421 tcttcttcac cgattggcgt ttcaagggaa acaggttctt gcgcgatttt caagatttct
    63481 cggacttttt ctgttggaag atccatttcc gcaccgattt cttctggcgt aggttctctt
    63541 ccaaggtctt gcagcaattg acgttggata cgtatcaatt tattaattgt ttcaaccata
    63601 tgaactggaa tacggatcgt acgtgcttgg tcagcgattg ctcgagtgat ggcttgacga
    63661 atccaccatg tagcataggt tgaaaactta aatcctttgc ggtagtcaaa tttttcaact
    63721 gccttcatca gtcccatgtt tccttcttgg atcaagtcaa ggaactgcat gcctcgtcct
    63781 acataacgtt tagcgatact tactactaaa cgaaggtttg cttctgccag acgctgtttc
    63841 gcttcttggt cgccttcttc gattttcaat gctaacgcta cttcttcttc cgcagttaaa
    63901 agggagacac ggccaatttc tttcagatac atacgaaccg ggtcattgat ttttacaccg
    63961 gttggcgcag aaagatcttc tgtttttgct tgttcagctt ttttctcagc actcttcaag
    64021 ctgtgaattg atggatcacc attttcatcc accacactga tacctgcgtc ttcaatcttt
    64081 tggatcaatt tgtccatttc atctgcattc aaagtgaatg gtgtagcaag ctgattggat
    64141 aaatcatcat atacaacttg tcgtttgggt ttgttttctt tgatgaaagc tgctactgct
    64201 gcttcatacg tttttttatc tgttccattt gccataaaag aaggcccccc ttgagtagct
    64261 atttggcttg ttttaactgt ttcgttaaat tgatgatttc aatcgtcaat tcaagctcaa
    64321 gctgtttgtt tccagttcgt ctggcttctt gctgcatttg tttttttaat gttatttcat
    64381 cagctaagcc agatttgctg atcacatcta gcaagtcttg gatttctcgt ggcgaacttt
    64441 cttctgccac cggtaaatat tcgatttcca ccaccaaccg ccttaaattt tcatcttgca
    64501 aaaagtctaa aaactctgca aggttgaatt cataatgttg tgagacatat gcatcgaata
    64561 aaaaataaac ttctgcatat gcatcatgaa taaactgaaa ttctcgttct tttaaaatat
    64621 tacggacaga aacttcttta aatgcacgat ataatagaat ctgctctgcc ttttctactt
    64681 gcgtcaaagg tctctttttc cgtgcagttt cttgtctcgt aacctgagtg tgttgacttt
    64741 cctgaggttc ttgacgattt tgctgatttg tccttcgaag atcgcgcagt tgtttttgta
    64801 tcgtctctct gccgatctga aactctgttg acagttgaga tagatacata tcttgttcga
    64861 ttggtgaaac aactcggcta agttcaacta gtaattcttc gaggtattcg atcttgtctt
    64921 tttcattatc taaatttttc ccttgtttta aataacgtga tttaaaagta aagactgttt
    64981 cgcgcccatg agccactaat tcttggaaag atgcttctcc atacttacgg agatattcat
    65041 caggatcaag cttttctgga acgactacga cagaaagtgc taatcgactg ttttgccgaa
    65101 gcaattcaat ccctcgattg gttgcttcaa aacctgcgtt gtctccgtca tagcaaaaga
    65161 ccaattcttt cgtcagcttt tccatttgtt ggatctgttg agctgttaga cttgtcccca
    65221 tagaagctaa tccagttttg ataccagcct gccatgcagc aatcacatcc ataaaccctt
    65281 cgaacaacaa aatttctccg tttttccgga tatcgcttct tgctttatca agattaaata
    65341 aaacttgtct tttattgaac agttctgttt ctggactatt caagtatttg ggctgatctt
    65401 tttcctgccc tttttcaggt gcaagccagc gtcctgaaaa accgatcgtc tggccttgag
    65461 aattacgaag tggaaacatc actctttgat aaaaacgatc agacatacgg ccatcatcat
    65521 gcgtgacaaa caatccagag cgttctaaca attcatttga aattgattca ttcttgaata
    65581 cttgctgaag aaaagaacgc tcattaggtg caaaacctat gttgaattct tcaattaatt
    65641 ctaaagacaa cccacgactt tgcaaatatt ctaaagctgg ctgtccagct tttgtatgca
    65701 atagcatgtg atgatagatt tccgctgctt tttcatgagc agcaatcagt ttccccgtag
    65761 cagattcagg atttcggaca gctatagtac gctgacgcca ttgatcttct aacggtatct
    65821 gttcgaagtc tgccactttt accactgctt ctggaaaact tagaccttct agttcttgaa
    65881 gaaaagtaaa cacgtttccg cctttaccac aaccaaagca atgaaaaatc tgcttgtctt
    65941 ctgcaacaga aaaagacggt gtacgttctt catgaaaagg acacagcccc aagtaatttt
    66001 taccagactt tttcaactgg acatattgtc caatcacctc aacgatattc gtttgactgc
    66061 gtactgtttc gataacctct tgaggaatcc gttgagccat tttttcaccc cctgctctat
    66121 tgatatcttt tgggtttaac agaagatatt tcaggagaat aaccggtaaa aaatcgcact
    66181 aactgaaatc agtgcgattt taaaggacac aattatacat gtttgattgt atcatatttc
    66241 attgcttttt caagtataac acatttctat ctcattaagc atcaatttgc tactcttttc
    66301 agtaactgat tttgaatatc agccttccag tacaatactc gaagaacaat atttacgagc
    66361 atgaatacaa agaagtatcc tacgctctct cccaacccta agtttacttc caaaattcca
    66421 tggaacagca ttgcagctac atatatccct acgatcgtcg ttactgctag gaagatgcgc
    66481 aatgggttga agggataaca agcttttaca actgccatgc aactgatccc aatcagcaag
    66541 taatacatca aagtagtagt ttcagcattt ccccatgcga atgtttttcc aagtagatac
    66601 accgcaatga tattcactac tacaagcaag gcatttggta aagcattaat aagtgctgta
    66661 ggcaaataac ggtaagtgat ttttctttta tcttgttcaa aagataaaaa gaaggaagga
    66721 tacccttcaa tcgctaaatc aatcagcgtg atttggatag ggataaacgg aaatccaatt
    66781 gctgtaacgg cacatataat cgataaaata aatgaataaa tcgttttgat aaaaaagatg
    66841 cctgagactt tcgtcacatt gttgaccact cggcgtcctt caaacaggac ttctggcaat
    66901 gtagtaaaat cagagtccaa caataccaga ttcgcaatct gacgtgttgc tccatcacct
    66961 tctgccatag caatgctgca atctgcttct cttaacgcca aaacatcatt cacaccgtcg
    67021 cctgtcattg caaccgttct gccgctttct ttcaattcat ttaccagcaa tttcttttgc
    67081 tgcggactca ctcgtccaaa aaccgtatag cgatgagcag cttctcgcac ttccatttct
    67141 tcagtaattt gagaaagatc gacatacgca tcataattcg gtaatcctgc acgtctagcg
    67201 atattggaga cagtaaaagg attgtctcca gaaatcactt tcagatccac accttcctca
    67261 tgaagataag ctagtgtttc ttgagcgttt tttcggattg gatcatcgat ttcaaacaga
    67321 gcaaccggct caacaaatgc tggtttttct tcgttcaatt gctgatttgg agcgatcccc
    67381 aacataagta cgcgatagcc attcgcttgg gctgttttta gactgtccgg aagccgatct
    67441 tcttccacca aacgttcagg cgctcctacg tacacgactc ccagttctgg aaattccatc
    67501 gcgccccatt tgcgatcaga agaaaatgcg actttgtttg tcacatcata ccgattagaa
    67561 agctcaaaat aatctctcat agcccgcata gtgatgttgt tatcactgct ttctgccaga
    67621 tacgtcccca taatttctgg gatggctttc tcgtacagcg gatgtagtgc ttctacccgc
    67681 tggatactca ttttcccttc agtaatggtc cctgttttat ccaagcataa tgtatcgaca
    67741 tgggccagcg tttcaattga atacatgtct tgaacaagga ttcgtttttt tgccagtttg
    67801 gttacccctg tcgttaatgc aatacttatt agcaatacca aaccctttgg aagcatgcct
    67861 aacaaagctg ctgcagatgc aataacagaa attttgatag aagcatcgcg gatgaacagt
    67921 gcttccaaaa atagtatgat ccccaaagga ataatcacga aactagtgaa tttggatact
    67981 ttgcgaatcg aatctacaag ttcagaacga atagctttgt gagtctttgc ctcgctagtc
    68041 aagcggacag cataattatc tgcacctact cggataactt cagccatgac agaaccgctt
    68101 gtcaaaaagc ttccagaaag caattcagct ccagtttctt tgacaatcaa atcagattcg
    68161 cctgtcaaaa gagcttcatt tgcttctgcc cgtccagata taacctgtaa atcggctggt
    68221 atctgttctc ctgctccgat ttttaccaaa tctcctaata ccagctcttt agtagaaatc
    68281 gattgttctt taccgtcccg gatcactagt acttctgatt gggaaacaat cgataatttc
    68341 cgtacaagat ttcttgcatg tatttcttga taaattccaa taacgatatt gacgacaata
    68401 attgctaaat acgccatgtt ggaatacgca cctacaaata acagacaaat cccaatacca
    68461 aagttcagaa aattgaataa cgtcaatata ttatcgctga atatttgttt tgtacttttt
    68521 gatgcatctt cctggtagtc attttgctgg ccagaagcca ttttctgaac tacctcttgc
    68581 tgtgtaagtc cattcatttt ctttttctcc attttccgcc aactttcaaa aataatcacg
    68641 tgtttaagtc tagcgtgttc caagattaaa aacaacttgt ttttataaaa tttatagaaa
    68701 aacacacgaa tgattttaca aaaaagatgc caaccttttt gtttgaatca cccgtgtgct
    68761 gtttttatta tcaatctatc tttacttata ttgttcctcc aaacggctca tccaccaacc
    68821 tagtgctgta ccggctataa aaagaacagg acaagctagt accccagcaa ctcccaatcc
    68881 cgcgtaatcg gtaggaatga tcataatcgt tgaagcaaaa actacaccta aaatgaaatg
    68941 gaatagcttg ctatatgcct ttgagaaaat gtaatccatg atttttgaca atccaagaac
    69001 acaaagaatc ccaccaattc cgatcggcac aatcacttca agtcgtactt ctttaaatcc
    69061 gtcagccatc gctttataca atcccatata caccaaaaaa ttagaagggc tcaatcctgg
    69121 gacgatcata cccaaaccaa tcaatccgcc ggcaatcagc catgttccaa aattctgatc
    69181 gacttgcccg tcaaataatt gagcgccgta taataaaaac aaataagcca gaacaaaagt
    69241 aacaaccatg atgatcacat cagaagtatc tcttcctttt ttccctgctt ctttccataa
    69301 cgcaggaaca gttccagcga tacatccgac aaaaaaccat aatatcgttg tttcatacgc
    69361 acctaataaa aaactgacta aaaaagaaag tagaaagatg cctgtcactc cgcctagacc
    69421 aacaggcaga aaaaacaaaa cattttcttt gaaatttttt gtaaggtggg ctaaaaaaga
    69481 aatcagccgc tcatacatgc caaagacagc tgctaatgca ccaccgctga ctcctggcaa
    69541 gataaatcct gaaccgataa acattccctt cacaaaccgt aacatccagt ctttggttgt
    69601 gtaccccact tcttgatttt ccattttttg ccttttttcc actatttgcg tactccttct
    69661 ttcatttaat tttattcatg attgaacatt tcctgctatt ttacttaaag actttttcat
    69721 aggtctctat tattttttca ttcattttga caatatcagg ataaaacatg ttacctccat
    69781 tatgatagag atagccgcca ttgtaaagca aggactggat ccgccaatat cggtattgtt
    69841 tcccttcctc atttcctaaa agtgggctca gcacatcttt agaatacgct tctgctaatt
    69901 ctactgtgtt ttctccgcca tgatttttta cataggcaat ataatctttc ccaaaattat
    69961 atgcttggac agctgtccat aaatcgcatc cttgtttctt tgcttctact atcatttccg
    70021 ccaaatattt gacaccttga tcaatactgt cagctggatt atcaatgatc tcagcttctc
    70081 catgcagact ttccgagctc tgcatgagat ctgtcccttt tccttttgat tcggtcaata
    70141 ttatcgcctt gatcaaattc tcgtagtttt ccatatgata attttttgca gctgttgtta
    70201 tttctatttg atagctttcc actcgtttta ccgaacgata agttcttcct cataaaaaca
    70261 acaccgcacc aataaccgtc agaatgatca tgtaaaaaag aatacggata atagataatc
    70321 gttttcgctt catttcatca cctagttatt ttgacatctt tttttatctt actatttctt
    70381 tatcttcttg gctatttctt ccttgatttc tgtgtacgcc ttaagaaaaa atgaattttc
    70441 ctgtattttt tgacatctta cgaaaatgat cgttgttttt cagtaaaaac aattgcccaa
    70501 tgactgatat aatcgttata ctttacacag taggtgatta tgtgaaaaaa gaaaagaaat
    70561 atgcaaaaat gccagatggg agcgaaattt actatgaaaa gagcggtcaa ggcttccctc
    70621 tctttttact gcatggcaat gacggcagcg gccgcttttt ttcagaacag gtccctgtac
    70681 tcgaacgcta ctataccgtg tatctagtag atagccgagg acatggtcgt tcgacaaacg
    70741 aagcatctat gttgaatttt cagttaatgg ccgaggatct gaatacgatc atgttgctgg
    70801 aaaaaatcga tcaggcagat tttcttggat ttagtgatgg agcaaatcta gcactggtat
    70861 ttgccagcag cttccctaaa aaagttcatc gattgatttt aaattctgga aacacgcttg
    70921 ttaaaggcgt tcgtttttct gccagagtca tcagtaatat tcattatgca tgggtatggt
    70981 tactctcttt atttcgccca agtcttcgca aaaatctttt agttatcaaa cttttgctgc
    71041 atgacatcgg gcttacagaa aatgatttaa aaaaaatcaa ctcccccact ttgatcatcg
    71101 tgggaaaaaa agatgtaatc aagctaaaac attctttgta tatagctaaa actataccaa
    71161 aagcttcctt tgtgcttgtg aaagaacaag gacatgaatt agctagaaaa gatcctgaac
    71221 gcttcaatcg agaagttctt caatttcttt ctgaaacata actattagga atagaaggtg
    71281 atttatttgt taaaaaatac catacaatgg ctgaaaaacc acctaggact gttcaaaaca
    71341 atctttttga tttctgtcat tgtcattatt gtcggtgaat tgatgtccat cggtaaaact
    71401 ctatctatcc agcaattagg agaaacgttt gccactatac ctgtatggaa aagcggacta
    71461 atgctattga ttggtcttat ctctgtgtta cctatggttg gttatgatat tattttgaat
    71521 cggatgcttg aacaaaagca gaatcctcgt tacctgtttg agacaagctg gctgatcaat
    71581 acgatcaaca atattgccgg tttcggcggg tttgttagtg tggggcttcg ctctgaacta
    71641 tatggacaaa agaaagagag cagaaacgtg atccaagcat tatcgaaagt tttcttgttt
    71701 ttgatggctg gactttctat ttacagtctg ctttcttttt tacttgtagt cttcacgcca
    71761 gttgctcctt ttttgaagca atattggata tggttgatcg gcggcggtct atattttcct
    71821 gtcgtctttt tatttactac tttcaaaaag aaaggattcc ttggtggtac ctcacttaaa
    71881 actcaaggac agctattgct agtttcattg ttggaatgga ccggtgtcct gtttagtttc
    71941 ctttctgttg gttatttaat ggaaatccgg attgatctgt ggcaaacaat tccattattc
    72001 attgctgctt cagtgatagg aatcgtgtct atgattccag gagaaattgg gagtttcgat
    72061 gtaatgatga ttatcgggtt gtccgctatt ggtgtgccaa gagaaatagt cgttgtgtgg
    72121 atcttgctct atcgcctgtt ctactacatc attccgtttt tgattggaat cgtctttttc
    72181 ttcaaaaata tcggttccac ttttgatcag cgttattctg gtatccctaa acaattagca
    72241 acagaaattg cgcataaaat cgtggttgtc ttattatact tttctggaat catgctagta
    72301 ctttctgcca ctatcccgca agcttttaca gagtttcgct ggcttcatag tttgaaccca
    72361 ttgaaatttc attttattat ccaatttcct agtatactat tgggatttct tttaatcatt
    72421 atgggcagag ggatcgcagc acgtgtgaaa cgagcatatt tgcctacaat cttcttgatt
    72481 gttctagctt tactttatgt tttattaagt gattttagtt tcacaccggt ggttttcttg
    72541 tctattctgc ttctgatcat tcttgcttct aaaaacgaat tatttcggga acaattgatc
    72601 tattcatggg agtggcgtac gatcgatgga atcattattg gtgcactgtc cttgctttac
    72661 atcgttattg gggtatacaa tctgccggat ttccctcatc ggcgtcacca ttttatttcc
    72721 tttttcttat ttccttctga aaagatttgg ttttcgggat tgttggccat catcgctgtt
    72781 agtttcatga tcgttctatt tgttcatttc cttcaaggag aaaagaaaca gattggcgaa
    72841 gcctttaatg aagaaaaggc cctgaagatc cttacaactt atggaggaaa ctccgatagt
    72901 caattgatct tcttaaaaga taagcggatg tttgcctatg aaaaagacgg tgaaccaact
    72961 gttcttctcc aattcgcttg cttcaataat aaatgtattg taatgggtga tccttctggt
    73021 aagaaagaag attttccaga agcaatcgaa gcttttatcg aagaaacaga tcgactatgc
    73081 tatttaccag ttttctacga aacaagcgaa gagattgtta tgattttgca tgaattcggc
    73141 tacgatttca ttaagatggg ggaagaagct tatgtagacc tgaatagttt tacaacttca
    73201 gggaaaaaaa tgaagggcac tcgtgctgtc ttgaatcgga tcgaacgaga aggatttaca
    73261 tttgatgtct tacaaccacc attttctact gagcaaatga gtactttcaa aaatatctct
    73321 gataattggt tgggttcacg aaaggaaaaa gggttttctt taggcttttt ctctgaagat
    73381 tatctacaaa gagcgcctat tgcagttgtc aaaaataccc aagaagaaat tgtcgctttt
    73441 gcaacgatca tgcctactta cacgaacaat aaagtaggaa cgattgatct gatgcgatat
    73501 gattctaaaa aagcaccttc tggcagtatg gatttcttgt tcttgcattt gtttgcctat
    73561 atgaaagaag aacatatcca atggttcaat ttagggatgg cccctctttc aaatgtcgga
    73621 acttccagaa agagtttttt acaggaaagg attgcatact tggtttacga gtttggttct
    73681 catttttatt ctttccatgg attaagagaa tataaaagca agtacgctac aaactgggtc
    73741 tcacgttata ctctatattc tagagatagt tggattgcat atgttatgat tgctttgctg
    73801 attattgata atgcgccagt tgagcagaca caaggtagaa taagtggttt gaaaaaatgg
    73861 atacgaagaa agtattaaaa actgcatacc gatagatgaa cactctcatg atgaataaaa
    73921 gagactgaag taaaacgaaa gttcatgctt ctacaatctc ttcttataca attgaagaac
    73981 aaagagcggt ccaaacagaa atcctgtttg gaccgctctt ttattttaaa catttttcat
    74041 taccaaaata ttcgagtcgc gtttcttatg cttcgatctc tgtaacgata cctgaaccta
    74101 ctgtccgtcc gccttcacga attgagaatg tcgttccgtt ttcaacagcg attggatgga
    74161 tcaagtcaac atcaatcgtc acgttgtcac caggcatgac catttctgta tcttctggca
    74221 atgtgattgt tcctgtaaca tcagttgtgc ggaagtagaa ctgtggacga tagttgttga
    74281 agaatggagt atgacgtcca ccttcttctt ttgtcaacac gtacacttct gctttgaatt
    74341 ttgtatgtgg agtaatggaa cctggtttag caagtacttg tccacgttcg atatcgtcac
    74401 gttggatccc ccgtaacaac acgcctacgt tatccccagc ttccccgtaa tctaacgttt
    74461 tacggaacat ttctacacct gttacaactg ctttttgtgt ttcaggtttg atccctacga
    74521 tctctacctc atcaccgaca cgaacagcac cacgatcaat acgacctgag gcaacagttc
    74581 ctcgacctgt aattgagaag acgtcttcca ctggcaatag taatggttta tctgtatcac
    74641 gttctggtgt tgggatatat tcatctactg tatccatcaa ttccataata gcagcttcag
    74701 catctggatc gccttgcaat gcttttaatg cggaaccttt gatcacagga gtgtcatcgc
    74761 ctgggaaacc atattcgctc aataactcgc gaacttccat ttctaccaaa tcgatcaatt
    74821 cttcatcatc gactaaatca actttgttca agaaaacaat caggtatttt acaccaactt
    74881 ggcgtgacaa taaaatgtgt tcacgtgttt gaggcatcgg accgtcagtt gcagaaacaa
    74941 ctaagattgc accatccatt tgtgctgccc ctgtaatcat gtttttcaca tagtccgcgt
    75001 gtcctggtgc atcaatatgc gcgtagtggc gtttttctgt ttcatattct acgtgtgcgg
    75061 tattgatcgt aatcccacgt tcacgttctt ctggagctgc atcaatacta gcatagtctt
    75121 gaggatttgc taggcctttt ttgcctaata ctgtagtgat cgcagcagtt aaagtagttt
    75181 tcccatggtc aacgtgtcca atcgtaccaa tgttaacgtg tggtttactt ctgtcataat
    75241 gttcttttgc cataatttac aaacctccta aataatatag atcactgaaa aatttcagct
    75301 ctcacctttt tattatagtc tttttttgac taaatcaaaa agaatacgtc cgacttactt
    75361 tttgtaagat tcgaactaag cttattatac gtcaatattg gtcaaagtca actaagagca
    75421 ctcgtctgtc ttatacgcga aaaaagtacc ttttataaat aaaaaaatgt gttttttgag
    75481 aaaaacttca agatagtcct taactttctt ttgaagcaat aagaacaaaa tgaggaaaag
    75541 tttaaaattc agctgttgcc aacaactttc aatagacgaa atcttctatt tcgttaataa
    75601 taaggaaaga ggtgaagtac atgaaaaaat taattggcgt acttgtcgtt ttaggcatat
    75661 ttctcggcgg aagttttgca gcttatcatt atttttacgg cggcgaagct tattatacaa
    75721 aaatcacgac gaatggcgaa atgtcagcta cccaagcaga taacggagag aaattcacga
    75781 cttataccta caagcaagcg gcttataata aagatggtga aaaaaaacag gtgacgcttc
    75841 gcgaagaaag agaacatcca ttaaagatga atgcgtatct taaactcaaa gtcaatcctc
    75901 gtaaaggtgt attaagctgg gaagaaataa aggcttctga agtgcctaaa aatgcggcag
    75961 aaaaaatcga taattagtaa atggtcttaa aattttatta gataaaaaga ggctgggaca
    76021 gaagcgttta actccaagaa ataagaagga gttgcttctg cttccgccgt ttatacgtgt
    76081 ttagagcgtg agacaaaagt gttctttact tttgtctcac gctctttttt tgttatattc
    76141 gtcttttccg atccttctct tgacacaatt catttaactg atcactggta cttgaacacc
    76201 taatattcta gtcattttta taaacgattc aatacttcat ccacattttt gatcatcacg
    76261 gatatcgtac catccggatt attcacaaat tcaatcaaat ctgggttgcg atatacatct
    76321 actggtacga tcagttcgat cccgtttgat agcttgaatt tttgttttcc aaattttttc
    76381 tcagaaattt cttgtacttc ttttacagga ggtgcttctg ggacaaatcc ttgctcttgt
    76441 acttcttctt taaaagccat ttttgccgtg atgttttcct tgaagacttt ttccgcaatc
    76501 atctcatgat ccaaccgacc tttttcttct atcgtatcat agacagcttc tttcaaatca
    76561 gccatgatat caaattcttc tgtctcaaat ttttttccaa gatgcttggc tactttttgg
    76621 atcactttta cattttcttc taaagaaggt gcaggaacac tttcgatcac tcttccagaa
    76681 aaataaagct cttttttgcc agaaaactca tatttttttt caattaattc gtaggtccaa
    76741 tcagtcaaat tcacagcgaa tgcttcatct gctttttgtg ttttcgcccc tagaattgct
    76801 cgattcaaaa tcaagcggtt gtcgatccct gcttcgtctg cttcaacaaa gtgagtgtaa
    76861 gcctcacgat aattgacttt caataaagat aagtacatca ctgtatctaa ttcatacaag
    76921 acaacaaata catctgcact aggtgcttct tcactttctt ggtagatatc gaaccaccgc
    76981 tgaacgattg cttcacttgc ttccacaaaa ttttcttgag cgattttaag tgattcaacg
    77041 atcatgcttt cttctgctag ttgtccagtt tttgtttgag cagaagagat tttttggatc
    77101 ttcttttgaa gaaaatcacg aatatattct tttgtcagat ccagctcttt ttgtgaaaag
    77161 actggagaac cagtttctct gtcaataata tgcagaattg ctttttttaa atagatatcc
    77221 attttctcat tcccttctgt cttagtttac ggaaaaaaaa tcaaaaatac cagaatcaaa
    77281 atcattttct tttaaacaaa aatttcaaga gtagaaacaa aatggattct ttttttcgaa
    77341 aaatgcgcta cacttatagt tgaacgaata aaaaaggagc tgaaagtatg aattcattaa
    77401 caagcactta tcgtctatca aatggttacg aaatcccggt agtaggattc ggcacatggc
    77461 aaacacctga tggggatgtc gctgtttcct ctgttaaaga agcgctggct gcaggttatc
    77521 gccatatcga tactgcccaa ggatacaaga atgaagaaag cgtcggccaa gcgattaagg
    77581 aaagtggaat tcctagagaa gagatattct tgacaacaaa attatggaat gcaaaccaca
    77641 gctatgaact tgtgatgtct tcttttgaag aatcattgaa gaagctccaa acagattatt
    77701 tagatctgtt tttgatccac tggccaaacc ctgttgcttt tcgtgataat tgggaacaag
    77761 caaatgcaga tacatggcga gcatttgaag aactgtatga agcaggtaaa atcaaagcaa
    77821 tcggtgtgag caatttctta ccccaccatc tagatacact agcaaaaaca gcaaaaatta
    77881 tgccgatggt caatcaagtt ttccttgcac caggcgaatt acagacaact gtagttgagt
    77941 atgcaaaaaa acatgatatg atcctcgaag catatagtcc attaggcact ggtaaaatct
    78001 tcgatgtgcc tgaaatgaaa caaatcgctg aagcacatga caagacgatt gcacaagttg
    78061 ctttgcgttg gtcgctgcaa cacgaattcc ttcctcttcc taagtcagtg acaccaagca
    78121 gaatcaaaga aaatactgaa ttatttgatt ttgaattaac agaagaagaa atgaaacaaa
    78181 tcgatcagtt agatggtgta gtcggcaaag caaaagaccc agatactacc caattttaag
    78241 tcgaaaggtg attttatgga tccaatcgaa cgcttgaatt ctctctcgga agaagttacc
    78301 cagacttttc attccgattt tgtctttttg atcgatgcag agaaaatcca acattttcct
    78361 gcaagaaact ggacacatga tcaaatcatc gaagaactga aaaaacggtt cgatcattcg
    78421 ctaatggtca aaccctggca cgaacatgaa gtcatttatt cacctgaact acctgttttt
    78481 gccttgatac cgaaaaaata gaaa
//
LOCUS       DAFCHQ010000005        69669 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000005 DAFCHQ010000000
VERSION     DAFCHQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 69669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.005"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(606..962)
                     /locus_tag="LNC71_000377"
     CDS             complement(606..962)
                     /locus_tag="LNC71_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBK6465475.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /gene="spx"
                     /locus_tag="LNC71_000378"
     CDS             complement(976..1386)
                     /gene="spx"
                     /locus_tag="LNC71_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK6465476.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="LNC71_000379"
     CDS             complement(1732..3918)
                     /locus_tag="LNC71_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBK6465477.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="LNC71_000380"
     CDS             4136..4819
                     /locus_tag="LNC71_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBK6465478.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="LNC71_000381"
     CDS             complement(4900..5619)
                     /locus_tag="LNC71_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6465479.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="LNC71_000382"
     CDS             complement(5931..6137)
                     /locus_tag="LNC71_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465480.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="LNC71_000383"
     CDS             6382..6984
                     /locus_tag="LNC71_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK6465481.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8405
                     /locus_tag="LNC71_000384"
     CDS             7125..8405
                     /locus_tag="LNC71_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6465482.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8418..9146
                     /locus_tag="LNC71_000385"
     CDS             8418..9146
                     /locus_tag="LNC71_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6465483.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9215..10738)
                     /locus_tag="LNC71_000386"
     CDS             complement(9215..10738)
                     /locus_tag="LNC71_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK6465484.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10990..12171
                     /locus_tag="LNC71_000387"
     CDS             10990..12171
                     /locus_tag="LNC71_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6465485.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12231..13859)
                     /gene="treC"
                     /locus_tag="LNC71_000388"
     CDS             complement(12231..13859)
                     /gene="treC"
                     /locus_tag="LNC71_000388"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBK6465486.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14310..17021)
                     /locus_tag="LNC71_000389"
     CDS             complement(14310..17021)
                     /locus_tag="LNC71_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465487.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17031..17732)
                     /locus_tag="LNC71_000390"
     CDS             complement(17031..17732)
                     /locus_tag="LNC71_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465488.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17918..18517
                     /locus_tag="LNC71_000391"
     CDS             17918..18517
                     /locus_tag="LNC71_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6465489.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18598..19611
                     /locus_tag="LNC71_000392"
     CDS             18598..19611
                     /locus_tag="LNC71_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6465490.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19719..20636
                     /gene="rbsK"
                     /locus_tag="LNC71_000393"
     CDS             19719..20636
                     /gene="rbsK"
                     /locus_tag="LNC71_000393"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBK6465491.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20664..21059
                     /gene="rbsD"
                     /locus_tag="LNC71_000394"
     CDS             20664..21059
                     /gene="rbsD"
                     /locus_tag="LNC71_000394"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBK6465492.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21059..22432
                     /locus_tag="LNC71_000395"
     CDS             21059..22432
                     /locus_tag="LNC71_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6465493.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22789..24933
                     /locus_tag="LNC71_000396"
     CDS             22789..24933
                     /locus_tag="LNC71_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBK6465494.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24985..26025)
                     /locus_tag="LNC71_000397"
     CDS             complement(24985..26025)
                     /locus_tag="LNC71_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6465495.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26302..28104)
                     /locus_tag="LNC71_000398"
     CDS             complement(26302..28104)
                     /locus_tag="LNC71_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465496.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28118..28870)
                     /locus_tag="LNC71_000399"
     CDS             complement(28118..28870)
                     /locus_tag="LNC71_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465497.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28879..29241)
                     /locus_tag="LNC71_000400"
     CDS             complement(28879..29241)
                     /locus_tag="LNC71_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6465498.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29417..30289)
                     /locus_tag="LNC71_000401"
     CDS             complement(29417..30289)
                     /locus_tag="LNC71_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBK6465499.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30340..30828)
                     /locus_tag="LNC71_000402"
     CDS             complement(30340..30828)
                     /locus_tag="LNC71_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBK6465500.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30966..31757)
                     /locus_tag="LNC71_000403"
     CDS             complement(30966..31757)
                     /locus_tag="LNC71_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBK6465501.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31812..32420)
                     /locus_tag="LNC71_000404"
     CDS             complement(31812..32420)
                     /locus_tag="LNC71_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBK6465502.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32413..32730)
                     /locus_tag="LNC71_000405"
     CDS             complement(32413..32730)
                     /locus_tag="LNC71_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6465503.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32886..33614)
                     /locus_tag="LNC71_000406"
     CDS             complement(32886..33614)
                     /locus_tag="LNC71_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBK6465504.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33773..34654
                     /locus_tag="LNC71_000407"
     CDS             33773..34654
                     /locus_tag="LNC71_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6465505.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34891..35934)
                     /locus_tag="LNC71_000408"
     CDS             complement(34891..35934)
                     /locus_tag="LNC71_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6465506.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35937..36605)
                     /locus_tag="LNC71_000409"
     CDS             complement(35937..36605)
                     /locus_tag="LNC71_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6465507.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36747..39083
                     /locus_tag="LNC71_000410"
     CDS             36747..39083
                     /locus_tag="LNC71_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6465508.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39198..39509)
                     /locus_tag="LNC71_000411"
     CDS             complement(39198..39509)
                     /locus_tag="LNC71_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465509.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39690..41165
                     /locus_tag="LNC71_000412"
     CDS             39690..41165
                     /locus_tag="LNC71_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBK6465510.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41212..41796
                     /locus_tag="LNC71_000413"
     CDS             41212..41796
                     /locus_tag="LNC71_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBK6465511.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41798..42217
                     /locus_tag="LNC71_000414"
     CDS             41798..42217
                     /locus_tag="LNC71_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBK6465512.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42498..42782)
                     /locus_tag="LNC71_000415"
     CDS             complement(42498..42782)
                     /locus_tag="LNC71_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBK6465513.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43134..43844)
                     /locus_tag="LNC71_000416"
     CDS             complement(43134..43844)
                     /locus_tag="LNC71_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBK6465514.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43841..45313)
                     /locus_tag="LNC71_000417"
     CDS             complement(43841..45313)
                     /locus_tag="LNC71_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6465515.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45451..45924
                     /locus_tag="LNC71_000418"
     CDS             45451..45924
                     /locus_tag="LNC71_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6465516.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(46077..46628)
                     /locus_tag="LNC71_000419"
     CDS             complement(46077..46628)
                     /locus_tag="LNC71_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6465517.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46733..47263
                     /locus_tag="LNC71_000420"
     CDS             46733..47263
                     /locus_tag="LNC71_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBK6465518.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47746..48189)
                     /locus_tag="LNC71_000421"
     CDS             complement(47746..48189)
                     /locus_tag="LNC71_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6465519.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48205..49365)
                     /locus_tag="LNC71_000422"
     CDS             complement(48205..49365)
                     /locus_tag="LNC71_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6465520.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49872..51572
                     /locus_tag="LNC71_000423"
     CDS             49872..51572
                     /locus_tag="LNC71_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465521.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51674..52507)
                     /locus_tag="LNC71_000424"
     CDS             complement(51674..52507)
                     /locus_tag="LNC71_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBK6465522.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52811..54976)
                     /locus_tag="LNC71_000425"
     CDS             complement(52811..54976)
                     /locus_tag="LNC71_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6465523.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55236..57530
                     /locus_tag="LNC71_000426"
     CDS             55236..57530
                     /locus_tag="LNC71_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK6465524.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57523..58188
                     /gene="pgmB"
                     /locus_tag="LNC71_000427"
     CDS             57523..58188
                     /gene="pgmB"
                     /locus_tag="LNC71_000427"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK6465525.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58181..59188
                     /locus_tag="LNC71_000428"
     CDS             58181..59188
                     /locus_tag="LNC71_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6465526.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59210..60229
                     /locus_tag="LNC71_000429"
     CDS             59210..60229
                     /locus_tag="LNC71_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6465527.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="LNC71_000430"
     CDS             complement(60510..61877)
                     /gene="nhaC"
                     /locus_tag="LNC71_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK6465528.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61986..63407)
                     /locus_tag="LNC71_000431"
     CDS             complement(61986..63407)
                     /locus_tag="LNC71_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6465529.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="LNC71_000432"
     CDS             complement(63650..65527)
                     /gene="tdc"
                     /locus_tag="LNC71_000432"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK6465530.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65818..67074)
                     /locus_tag="LNC71_000433"
     CDS             complement(65818..67074)
                     /locus_tag="LNC71_000433"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBK6465531.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(67186..67391)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            67654..68955
                     /locus_tag="LNC71_000434"
     CDS             67654..68955
                     /locus_tag="LNC71_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK6465532.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(69024..69623)
                     /locus_tag="LNC71_000435"
     CDS             complement(69024..69623)
                     /locus_tag="LNC71_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6465533.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        20906 a        14094 c        12343 g        22326 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7381 ttttaggaga tcacttaaca agcatcggcg atttttttgt tttgaaatct tcggttattg
     7441 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7501 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7561 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7621 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7681 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7741 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7801 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7861 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7921 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7981 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     8041 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     8101 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     8161 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     8221 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     8281 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8341 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8401 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8461 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8521 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8581 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8641 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8701 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8761 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8821 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8881 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8941 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     9001 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     9061 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     9121 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     9181 agccgatcca aacccttcgg ttcttttttg cctatcagtg caaaagcatt tcctgtgcaa
     9241 tttcacgtcc atccattgta tctggataaa atgttggcca attctccatt tctttcagca
     9301 atacttcttg actttcattt cctgcatata aatgaaaatg ttcggagtca gccggtgcaa
     9361 tcgtgtgatc actgaattga atatatctaa aagcgccact ttccttatct tctgcttcga
     9421 atagatacct tacgccacgg tttcctgcct cgtaggtcaa gatttctttc cctacataac
     9481 gataatttga acgtttcgtt gttccattct taaacgtaaa tgccatctct ttctcactga
     9541 tcttgattga agcaatatct gtttgatagc ccttcgtata atattcttta tattcttgtg
     9601 ctgtcttatc ctgattcaat ttttctttat attcaaaaac aggatcaaaa gtcccatctt
     9661 ctacatacgt atctactgtt tgccaagttc ctgcccaatc tgaaagagag cgatcttgta
     9721 catcctcgtc ttcaaaataa ccgttgtaga cgctcttttc ttttttgggg gtcaaagtat
     9781 cttctagcgg gttttctgtc tctgtcgttt ttttcaacgc ttcgaggttt gcttccatga
     9841 tagaaagata gttttctcca ttttctattt gttcattcgt caatccttcc aaaggattca
     9901 atacttctaa agatacacct gcttcgtttg ctaacgttct ggcaatacta tctttggcgt
     9961 tttcttcaaa ataaatatag ttgattccat gttctttaac aaagtgtttc agttcagcaa
    10021 ttctcgccgc agacggttct tcactagaag aaagaccagc aatcgatact tgattcaatc
    10081 catattctaa cgctagatag ttgaatgcag catgttgcgt gacaaaagat ctttgttttg
    10141 cttgcttaaa ggtttcttga taaagctgat ggagtgcttg aagtttctcg gtataggctg
    10201 ctgcattttt tgtccatact tcctgttttt ctgggtaagc ttcaatcagc tgatcacgta
    10261 tgtttgctac ctgcttgatt gctaaacttg gagctagcca tacatgtggg tctagctcat
    10321 gtgtatggcc ttcttccccg tgttcatggt catgatcatg tccttcttca cttcccggaa
    10381 gtaaaagcat accttctgtc gcttttatcg tatggacatt gccttcttgt aatgtttttt
    10441 ggattgcggg gacccatgtt tccatgtttt catcattata aacaaagata tctgtatctt
    10501 ggatctttgc catgtcttta gcagaaggtt cataatcgtg ggaatcagtc cctgctggaa
    10561 tcaataactc tacttctcct tcatccccga cgattgcttt tgtaaaatca tacatgggat
    10621 aaaaggtcgt catcacgctc aatttttctt tactgtcctc ggctttccca cttgccccgc
    10681 aagcagttaa catacctgct aaaaccagca tggccaatat acctatcaat cgtttcattt
    10741 atgacactcc tcttctcatc ttctttcatt tgaatgttcc gattcgcttt tcgcaaaccg
    10801 taatagttac gttttagcaa caggatttaa tttatcatat ggttaaaaga actgtcaagc
    10861 gttttcttat tttaattgat gagaaagttt atatgaatga ttgacgatat ttttgcaatc
    10921 aacctctatc attttccttt tatacccgct tttgtctata atgaatttat agataaagga
    10981 ggaaaaagag tgaaaaaaga aacagataca acgaatcgtt tgatccgctt tttaggagga
    11041 aaaacctctt actatgtttt aggaatagtt atcttacttg cgctgatcat ttattttttc
    11101 catcagatct cgtttatatt caagcctgtc ggaatcatta tcgctactat cttgccccca
    11161 ttattgtttg cactgatctt gtattatctt ttggaaccag tcatcaaacg tttgagcaag
    11221 cacctttcga ggaacttgtc agtcattatc gtttatgtga ttattttatt tttattgggg
    11281 cttggaagca tatggcttat tccttttctt gaagaacagg caaagacatt gatcagtagt
    11341 ttgccagatt tgtttcaaga ttttcaagag agtctccgac aatttttgga gaaaacacct
    11401 tttgccgatt catttaatca atttttcgct tctattgacg acgtgacgtc tgatatagct
    11461 ggatttattg gaaattattg ggaaagtggc gcacaacgga tcagcaacat tttttcaact
    11521 gttacagcta tttttattac tttatttact ggtccaattg tcgctttttt ccttttgaga
    11581 gatcctcaaa aattttatca gtcagtttta gcgattgtcc caccagcatt tcgtacagat
    11641 tttaaaaatt tgacaaagat tgctaatcaa caattgggtt catttctaaa ggggcaaatc
    11701 attgcttcat ttatccttgg agcagtctac tgggtttgtt tcttactgat cggattagaa
    11761 tttgctagtg ttttagctat ttctgctggg ttgttatgta tcattccata tattgggccg
    11821 tttatcgcct tctttccagg tttgatcatt gcattccaag actctacttt tatggctgtc
    11881 aaatttgtca ttgtctggtt tgccgtacaa cttctccacg gtgacttggt gattcctcga
    11941 gtaatgggtg atcgtttaca gatccatccg attacgatct taatcgtttt acttgtcatg
    12001 ggtgatctaa tgggaattgt gggcgttatt tttggtattc ctatctatac gttagtcaaa
    12061 ttactcgtta cttttatttt ccgtaaattc aaacaacgct acaataaatt ttatggcgat
    12121 aaaggagagt atgaaaactc ttcattttct gaagaagatt attttaaata aaacacaaaa
    12181 cgtcgatctt acgagaatag ctcgtaggaa cgacgttttg tgtttttgtt ttaatttttc
    12241 agcaaagcaa ttgcctggta tggttttagc tccagacttt gatgaagaac agtatcggaa
    12301 taattatcga taagaatatt ggcttctgtg aatccttctg ggatggcgat ccttgtatct
    12361 ttatcataga aatttgttaa tacaagcagc tgctgtcctt caaattggcg aagaaagcca
    12421 taaacttccg gatgatttgc ttcataagca tgataatctc cttgtgcaac gattggatat
    12481 gtttttctca gccgaatcaa tttcttgtaa taggacaaaa tagaatcttc agaagatagt
    12541 tctttttcta cattaatctc ttctagcttg cctccaactt ttaaccaagg tttccctgta
    12601 gtaaatcctg cttgttctgt gtctgaccat tgcatgggtg ttcgagagtt atcgcgagat
    12661 tttgctttga tccgacgaaa cgcttctttt tctgtgaatc cttgttcaag gagtaactta
    12721 taagcattca aactttctac gtcaatatag tcggaaatcg atgaatagtc tgggtcgatc
    12781 atgccgattt cttctcccat ataaatatag ggcgtcccac ggttcaaatg gatcatggtt
    12841 gctagcatct ctgctccttg ttttcgatac tgatcgatag ctacaaatcg attgagcgca
    12901 cgtggctgat catgattatt aaaaaataga gcattccagc cgttaccctc actcatcttt
    12961 tctccccatg tatgaaatag tcgctttaat tctgcaaaat caaatggagg agtagaccat
    13021 ttattcccat tttcataatc gactttcaaa tgatggaaat taaagaccat tgataattct
    13081 tctctactag gttctgtata caagatacaa ttttcgatcg tcgtagagct catttcaccg
    13141 actgtgatga tagtggaatc cgtcccaaac gttgcttggt tcaattcttt tagatacgta
    13201 tgggtgattg gtttgtccgt atattcaggt ttgccatcat tttctttatt atttttcaat
    13261 tgttcgtctt taccgataac attgataaca tcgaaacgga atccttttac ccctttttcc
    13321 atccaaaaac gaacaacttt gaatagttcc tctcttactg cttggttccg ccagttcaag
    13381 tcagcttgtg tccgatcata taagtgcaaa taatatttcc ccgtatctcc aaatggcgac
    13441 caagcacttc caccaaattt tgatacccaa tccgttggct cttctcttaa aatgaagaag
    13501 tcctgataat gctggtcccc tgctagtgct tttttgaacc attcatgttc tgtcgaaacg
    13561 tgattcagca ccatatcgag catgatttct atccggtatt tctttgcttc ggctattagc
    13621 tgttcgaatt cctccatcgt accaaagata ggatcgatgg ctgtgtaatc agagatatca
    13681 taaccgttgt ctttttgagg gcttggataa atagggttta gccaaatcat gtcgatgcct
    13741 agatcattca gatacgggag tttttctttg attcctttta aatctccaat tccatcacca
    13801 ttactgtctt tgtaagattt gggatagatt tggtaaatga ctttgtcttt aaatgtcatg
    13861 gatatttacc tccattcaac tgatttatta cctaccacaa cttatatata caagttatca
    13921 tttattatta taactgaacc gaaagcgttt gtaaaattta tctttataca ttctaagagg
    13981 tctgttgccc aataaaaaac aagagatcag acatgtattt gtctatcctc ttgtttaaaa
    14041 tagcaaaata ttcttgtttc ttctataaat ggtgttcaaa agcttttatc tacggcaatc
    14101 agtcttctca aatgtaaaac atgaggagct aattttattt gagtattctt tttgctcgat
    14161 gcaggacggc ttttttacaa cctctgattc atggtgttca atcagctgtc cttcggtatt
    14221 agctcaaaaa acggcaaaat atgaaaaact attttgccgt ttgtttctct aatactcagg
    14281 tcaaaccgct gatttcacaa cctctgtttt tattcattcg atttcaacgc atcaatcata
    14341 tcgatatgct ttaatttgaa atgggtaaca atcatcacaa taaccgtaaa tacgatagtt
    14401 aatcctgctg ataagacata agcccaccat gtgatcacta atggaaagat gacattctcc
    14461 atagacgctt gttgcaagat gaagtcagtc aatatatatc ctacaccaaa tcctccgatg
    14521 attcctaaaa gagtaaagat gatattttct cgaacaatgt acatcgtgac ttctttatca
    14581 aaaaatccaa ggacttttat tgtagataat tcgcgtacac gctcggaaat attgatatta
    14641 gttagattat acaagacgat aaaagccagc agtcctgata atacgacaaa gattaagaca
    14701 accgagtcta agttcgccat tgattcttct tgcgtttcga tttgtttgga catgaaagac
    14761 gtatttgtca cagcgtctgt ggcaagcagt ttctcggaaa gcatttcttc ttcttttgtt
    14821 gtcatcttat ctgtacgcag caaaaatgca ttatccatca tcgattgatc agtaactgaa
    14881 gcataatatt caggggacat ataaataaag ctacctagat agtttttcac gatttctttt
    14941 acttcgactt tccattcttt ttgatcttca tcataaagcg aaatatgatc tcctgtagat
    15001 aagtcaaata gttctgctac tttttgcgta atagcgattc ctgttttagg caaggagcgt
    15061 gggttctctt caagatcagt taaatggata aaacgatcca aatcagctgt tttacttggt
    15121 acgatcactg tcagactttg cgaagcatgt cctttttttc tcaattctac ggtttgagta
    15181 tgaatagcta ggatctcatt tactttttct tgcttctgca gcatatccgc tgctttttga
    15241 cgactatctt ccgtggatga atccaatgta acgattgctt gatagtcaat gatcggtcca
    15301 aattgtttgg ctgaaacaga acttagagaa tctttcaatc ccacgcctgc aaccatcagc
    15361 ccggcacatc cagcaattcc gataatcgcc ataagcattc ttgacttata tcggaagaga
    15421 tttcggtaac tgattttttg attaaagcta aggcgtgacc acagcggagt gatgtattca
    15481 agaaaaatgc gtttccctgg cttcggtgct ttcggttgaa gcaatgcagc aggccgttct
    15541 cttaaatctt tgaataaaac gatcattgca gcgccaaatg cggcaatgaa gaatgcggca
    15601 gctgcttgta aaatcggcgg ccagtcataa aaaataagcg cactcccaat atcataccgc
    15661 tcgttagaca gctcaaaaat gatccttggc aaaagattcg ttcctaatat cgttcctaaa
    15721 acaataccta tccctgaggc tagacttgca tagattgcat atttttttgc gatttcaact
    15781 ttcgtataac ctaacgcctt taatgtcccg atttccctgc ggttttcttc taccatacgt
    15841 gtcattgtcg taaaggtgat caatgcggca ataaagaaaa agaaaacagg aaaaacattg
    15901 gcaatcgcag cgatacgatc agacagatca ccaaattctt gaaaaccgac attttctgaa
    15961 cgttcattgt ataggtatga tggtttagtt aaattttcaa ttttttctgc atttcgattc
    16021 aattcttcct ctgcagaggc cagttcacgt tctttctcaa ctaattgcct ttcttgttca
    16081 gcaagttgag cttcttgttg agataatgtt tgaacagcga ttgttcgttg cgcttcagga
    16141 agctgctgga tagttgctga ctgttgcgct aactgttttt tagcatcttc taaccgtgtt
    16201 cttgcttgcg ttgtttgtgc tttgccatcc gctatttttt gctgtgccgg ttctaattct
    16261 tcgttggccg attttttcag ctcttctaag cgttcttcgg gtcgatcttg taaatacttt
    16321 tctgcctttt cttgattttg ctccatttta tctttgtaag ctttgctgta cgtattcaaa
    16381 ttctttacgt ttgtaaaatc aagatataag acagaataaa tatctgtttt aaaaacggat
    16441 tcaggtaaat agacaaaata atcgacgcta ccatttccga cgttcgcgta tcctctctcc
    16501 aaattgttga tgaacaatgg agaacggaca aagcccgtga tggtatactc tttttctttc
    16561 aattcatctg aatcaatctg ataaatatca ccgattttat acccttcatc ttccgctttt
    16621 tcatccaata caagctggtt tggtttttcc gggagttttc catcagttaa ttgtagcttg
    16681 ttctgcttgt tatttttgtt ataagaaaat atttgaatca cttcgttttt gccagtattg
    16741 gaataataga tctgttttcc tgtttctact tttgcatctg ggatcttttc tgcttgagca
    16801 atatcttttt gtgtcaagcc accagtagaa acgatttgca aatcagaaag tcctgctgta
    16861 ccaacatagt gatccatcgt tttttgcatg acaggtccag cagttttgac acctacaaat
    16921 aaaagtgttc ctagcatgac aataaggatg attgcaatga atcgtccttt agattgccga
    16981 atttcgcgaa aacctgcttt aagatatgtt cttgttttca taaaatcccc ctaccataca
    17041 atctcatcaa tggatgaagg atgatcattt aattcaacag accttacttt agcatcatta
    17101 atgtgaatga ctctatcagc gatgggcgct aaagcagaat tatgtgtaat gatcaaaacg
    17161 gtattgccgt gttgtctgct agcgttttgc aataatttca atacttgctt gcccgtttca
    17221 aaatccagcg cgcctgtcgg ttcatcacat aaaagaagtt tgggattttt agctagtgca
    17281 cgagcaattg aaaccctctg ctgttcgcct cctgataatt gggacggaaa gttattcaat
    17341 cgatgagcta ggcccacttg cctcaatacc tctacaggat caagcgcatc aggagaaact
    17401 tccgttgcca attccacatt ttcttttgct gttagattag gaacgagatt gtagaattgg
    17461 aaaacgaacc ctacatctgt tcgtcggtaa gcagtaagct gtttatctga aaattgcgca
    17521 atatctgtgt catcaataat gatctgacct tcatcaggtg tatccatccc acctaaaata
    17581 ttaagaatcg ttgactttcc tgctccactt ggaccaagta taatcactaa ttcgcctttt
    17641 tcaattgaaa aagaaatatc gtcatttgct gtaatggtcg tttctcccat tttgtatcgt
    17701 ttgtattcat tttttacttc gacatacttc actctgattc ctccattttc acgatatttc
    17761 tcgctatatc tatgcatcga atgcgttttc tcacacttat ttattttacc actgaatttc
    17821 tctagactaa agaaaggaat cctttaattt agttgacaaa taaacgattt atgatttact
    17881 tacataaaaa acattgttat ttgaaagaag gaacatcatg aatgatttaa aggaaaatat
    17941 gatttttgat ttatcaaaaa ctgcttttgt cgcgatcgac atgcaaaaag agatcgtaaa
    18001 taacggtaca ctctctccac acactgcagc atctattctg acaaacaatg atttgttagt
    18061 aagaacattg aaacgtaccg ctgccttgaa agttttagtg aacgtagaca tctccacttt
    18121 cccttatttg agtcaacaaa ctgatatggg tggaatgact gcttctgtac ctccagaatt
    18181 cactgattta cttttgaagg attctttgaa agatacagat aatatgttga ctatcacaaa
    18241 atacaatcca agtgcttttt ttggtacttc tcttgatctt caacttcgcc ggcgtggaat
    18301 cgaaactatc attttaagcg gtgtagcgac aacaaacggt gtatatgcta ctgcattaga
    18361 tgcctttcaa cacgggtacc atatcgtgct tgctgaagat gcttgcagtg atcgtgacaa
    18421 agaatcccat caattgttca taaaaaagat atttccgaaa actgcacgag tacgctcaac
    18481 gaaacaaata atcgaagcta tccaacaaag caaataagta ctggcacacg tgattttcat
    18541 ttacttcttt ttgatttcat gggaaaataa ccacaggatt tagataggag tatgaacatg
    18601 gtgaaaaaaa agatcacaat caaagaagtc gctaaacttt ccggcgtttc aatcacaact
    18661 gtctccttga tattaaatgg aaatacggag aaattcagtc cgaaaactgt tcagaaagtt
    18721 ttagctgcca aagaagaatt aggctatcag cctgactatt ttgctagaca aatgattacc
    18781 aaggaaacga aaacaattgg tgtactcgtt cctgatatca cgaatccatt cttcagtaca
    18841 ctgatgcgag gaattgagga tatcttgtat aagcagaatt tcgtcacgat attatgtaat
    18901 gctgattctg atcatcaaaa agaaatcgaa tatctagccg agctcacacg tcgaggtgtg
    18961 gatggattca ttatcgcgac ttctgctgtc tctacagatg caatcaacga aaatttgaaa
    19021 aaacaaggcc ggcccttcat tgttttggat cagaaaaaat ctgaaggatt tagtgatgcc
    19081 gtacgaacag acgattttcg cggtggttac ctagcgggca tgcacttgct tagtctagga
    19141 catcaaacga tcgcccttgt ctatcctgaa aatccaccgg aaaatgtcca cgctcggatt
    19201 gaggggttta aatctgctct tgatgtttac caaatccctc acgaccaact gatcctctta
    19261 cctacacagt tctcaaaaca aggtggctac cagatcacag cagaacttct tgattctgct
    19321 gcaactggtg tgtttgcttt aaatgatgaa ttagcttttg gattatatcg cggattagag
    19381 gaagctggaa aaagcatccc tgaagactac agcattattg gctatgataa tatagatatg
    19441 tgcgagtata tcaagccgaa actgacaacg atcgcacaac caatcttcga attgggacaa
    19501 acatctgcca agttattgtt agatcggatc caattcccag aaaaagaatg ggaagaaaaa
    19561 agattacctg tacgattaga aaaaagattt tcaactgcac ccttgaaata gctttcaagg
    19621 tgtgctatat ttttatcgaa ctattaaaac gttttactaa aacgttttaa aaaataattt
    19681 tcccatcaaa ataaatgaga tagttggagg tttgtaaaat gaacacggtt acagtaattg
    19741 gaagtattaa tatggacact acccttcgtt taaccaacat gcccaagcca ggcgaaacga
    19801 tgcatgcgca tgagattttt cacgctggcg gaggtaaagg tgccaaccaa gcagtggcag
    19861 ccaaacgttc aggcgcacgg acatctttta ttggaggcgt tggggctgac agcgaaggac
    19921 aacagttatt agatctactg acaaaagaaa acatcgatac atctgggata gctgaaatcc
    19981 aaggcgccac aaccggacaa gcaatgatta tggtagacgc agcaggagaa aatagtatct
    20041 tgatccatgc aggggcaaat aatgctttcc atgaacaaga agtcctaaaa aacaagcaac
    20101 tgattacaaa cagtgacttt attattgctc agtttgaaag tagcttagat gcgacgatcc
    20161 tagctttttc tattgcaaaa gacgcaggaa aaacaacgat cctgaatcct gcaccagctc
    20221 gtgaaacgat tcctactgaa ctacttgaaa agacagatat cattatacca aatgaaacag
    20281 aaacagaaat cattacagga atcagagtga cagatcataa cagtttggtc gcagcagccg
    20341 aaaaactcca tgaattaggt attggaacag taatcattac actaggcagt gccggcgctt
    20401 tctatcatac agaaaaagaa cacggtatcg tccctgcttt taaagtggat gccgttgaca
    20461 caacagccgc aggtgataca tttattggcg cattaagcag cacacttcag ccagacctta
    20521 gtaacttgaa agaagcgatc ctttatggaa atctagcctc ttctgttgct gtgcagtctt
    20581 atggtgcgca accttctatc ccttaccgcg aagctttaga acaatcagca gtctaaaatt
    20641 aacaatgaga ggaagaaaca aacatgaaaa aaggaaaagt aatcaattca gatatttcac
    20701 gagtaatcgc acaaatgggg cactttgata aattaagtat cggggatgca ggcatgcctg
    20761 tcccctttgg tactgaaaaa atagatttgg ctgtctcaaa cggtcttcca agttttttat
    20821 ctgttttaga aaacgtttta gaagaacttg aggtacaatc gattgaattg gcggaagaaa
    20881 taaagacaat gaacccggaa atattggaaa aaatcaaatt attgttacca gatacaccga
    20941 ttagctttat cccacatgaa gaaatgaaaa aagagttgaa taactgtcat gcatttattc
    21001 gtacaggcga aatgacacct tacgccaata ttatattagt gagtggagtg gtcttctaat
    21061 ggaaacgaca atgaatcctg aacagatggt actgtccgag cagattcata tgctgattga
    21121 acagttggga ggaaaagaca atattgattc tgtccttcca tgtgcgactc ggcttcgtat
    21181 caaattgaaa aaacagccag tagccaatgc agaaaaatta aaacagcaaa tcccttccat
    21241 taagtatttc attttactcg atagatatgc agaaataatc atggatcagg gattggctcc
    21301 tgcttacaag gaattgacgc gtcaaatcga tcattcagct ccgaaagaaa agctaaatcc
    21361 gatcgaaaaa ctatttggtt tgatatcgga aatctttacg ccgatcattc cgctgtttgc
    21421 cggttctggt atattaaaag gtctggttgt tttagcgact acgattggct ggcttagtga
    21481 aacaagcgga acttatcata ttttgtctgc tgcgtcgaat gccgtctttt attttctgcc
    21541 aatctttctt gctttttctg ctgccaaaac gtttaaagtg aatggatata ttgcggctgt
    21601 cattgctgca gcactgattg aaccgaatct gacctcttta ctttcagaca gtggcaaagc
    21661 agctactgat tttctgggga ttcctgtagt actgatgcag tatacgtcta ctgtcatgcc
    21721 ggcgatttta ggtattttct tttattcttt tgttgaaaaa ttcctcaaaa aatatattcg
    21781 cgaaaatatg caattagtat ttgtaccttt actttcatta gttatcacgg ttcctttgac
    21841 gttaatggta ttcgggcctg cgggtgttta ccttggcgaa ggtttagctg ccgcaattac
    21901 ttggatgatg gatattaacg gaccgatcag tggcgcaatc atcgctggtg gttggaatat
    21961 tttggtaata tttggtatcc aatgggctgt aaaccctgtc atgatcagta atatctctgc
    22021 ttatgggttc gaccgaatcg ttccgttgac aggagcagcc aattttggaa tggctggtgc
    22081 agctttaggt gtatttctgc gaagtaaacg ttcaaaaacc cgttcgatct ccggttctgc
    22141 ttttgcttcg attttacttg cgggagtgac cgaaccaact gtttacggaa ttgcgatccc
    22201 attgaaaaaa ccatttgttg ctgcttgcat tggtgcagct gctggtggag ctgtaatggg
    22261 atttgcccaa gtgaaagcga tcgcttttgt atttggtagt ctgaccacat tgcctgcatt
    22321 catttccggt acttttttct ggtacttagc aggactggct gttagtctag tcgtagcaat
    22381 gatcactact ctcgttagcg ggtttgatga agacctaatg tcttatgaat agttaatgaa
    22441 aaagactgaa gtatgagatg tttatcacat atttcagtct ttttcttttt aggatcatta
    22501 tttttataaa tttttgtgat actttccaaa ctttggtata atttacatat gcataaccat
    22561 ttttatcccc acttgtttat ccccaaacaa aggtggtcta tgcaataaaa tgaacgtcaa
    22621 agaaaaacga atgaatgctc gttgcttatt tacaaattgt tttatttaga atacacagac
    22681 agatttatga aaagaaaaag cttttcgaca cgattatctg ttccaaaaaa tgtctaatga
    22741 atgctgaata agacagaaaa tcaatgaaag gaaaaggact agtttattat gaaaaataaa
    22801 aaaacgatac gtttgctagc tgctacttgg ctgattgctc cgtctgtttt gtcgaccaat
    22861 accgtatttg ccgtagataa tcaaatagct gatcaaacag tcacaacaga tacgactaca
    22921 tccagctcag ctactagtga aacaacggat tcttttacag aagacccaac tggaacaact
    22981 aactcaacaa caggatcgac cgagccaact gaaattgatc cggctgatcc atcagaaaat
    23041 gaaaagccag ccgagacata ttcccttaca attgctcctt ctttgattgg taagatccaa
    23101 ctgactgctt cgacagcgga ttcccctgaa cagcaacctt tggatattac ggaaagtgga
    23161 acggtagaag gtttattagc ggaaacgaag gtaacttaca ctattacgcc agctgaaggt
    23221 gaacaaattg cttcagtcgc tgttaatggt gccaaagaag taaattatga tgcaaagacc
    23281 tttacagtag gtacgaacaa tttatctatt actgcagaat ttaaagctgg accggatact
    23341 tcaacagaga aaccaactga tcctagtgaa gaagtcccta gtacaccaga tccggaaacg
    23401 ccaaacaaac cagatgtggg taataacaac aataataatg gttcaaataa ttcaaacaat
    23461 aataacaata attcgaacag cgggaattct tcgaatcaaa caaacccatc tggatcgaat
    23521 aacaatggaa atactcagag agaaccaaac catccaaaca atggttcatc gaatggcgga
    23581 acgaacaatg cttcatctcg tccaaataca gcaaatcaaa gcggtataga gaatccatct
    23641 tctgcttctt ctgattttgt tgtaaaatca ccaattgatg ctgtattgcc tacaaataca
    23701 acgaatgtcc aacaagcaat cgttcgagaa gcattcaagc atcttggaaa accatatgta
    23761 tggggagcaa aaggtccaaa tacttttgac tgttctggtt tgacttatta cgtatatatg
    23821 aaagcaaccg gtcattatat cggtggctgg acaggcgaac agcaatatgc aggtacgcag
    23881 attccagtaa gtcaggccca acctggcgac ctcgtcttct ggggaccttc ttccggtgtt
    23941 actcaccatg taggtatcta tattgggaac ggacaattta tccatgcgcc tcaaccagga
    24001 gataaagttc gagtaacttc tatttcagat ttcacgcctg attttgctgt ccgagtaaac
    24061 ttagcaggtt tacctgctgc ttcaggttcg ctgacaggcg gctcatctat tttagatgga
    24121 ttagatggct cgttccattt cagtcaaaac caaacaactg atcagttctt gaaaaagatt
    24181 gcagatgatg cacaggaaat cgggcaaaaa gaaggaatct acgcatcagt catgatggct
    24241 caagctatcc ttgaaagtgg atctggaaac agcttattat caagcgaacc aaatcataac
    24301 ctttttggta tcaaaggatc atataaagga agcagcgtga ccttcaatac acttgaacaa
    24361 gatagttccg gacaaagcta ccaaatccgc gcacaattcc gtaaatatcc ttcttacaag
    24421 gaaagtttgg aagactatgc cgacttgatc aaaaatggac tgactggcaa tcctgatttc
    24481 tacaaaccaa cttggaagtc ggaaacaaaa gactacaaag aagcaaccaa atatttggaa
    24541 ggacgttatg ctacagatcg ccaatattca caaaaattga acgcgatcat cgaagcgtac
    24601 gacctgacaa aatatgatga accgaaaaaa gaagcagcaa caactgaaga agcaaacgct
    24661 gaaatcctca atatgtctaa gcgattcgat gtgccaatca aatggacaac agatggcgtg
    24721 ccaatcacag gattagaatt ttcaacgata aaacgcgtca acaacgcttc ttccatcttc
    24781 cgtgtcgctt caatctggga tttgtggaac aattttacgg caagacagat accagaaaca
    24841 actactcgta cagttaaagc tgcaactgtt cctgtactaa ccttgttaag tttagacaaa
    24901 tcattctggc ttgatctgcc tttaaagaaa tagaataaat taaaaagggg atgtgacact
    24961 gtcacactcc ctttttaatt tattctattt ctttatattt attttgtatt ccgttacaaa
    25021 ctcgcgtact tggttaggtt ccagagtgac atctccaaaa ctttcaagtc gctccgctcc
    25081 tggcagttct tgagtctcaa atgtgatacc gccatgattt actaatttat tcccgcgcat
    25141 ttccggtcca tcttctccaa aatttgctgt aaaaatcacg attcctgaag catctgtttt
    25201 tacttggatc gatacttttt catctgggct tgtcaattcc gcagcattta gtaaaccaga
    25261 ttgttttttc aacaaaaatg gatgatcgat gccgcccact atcattgatt ggcgaaaatc
    25321 actggcaaag acctcaccta attttttagg acgttggaag tcaaatgctg tgccacttac
    25381 tgaaactact tctcctgtag gcgttgtatc tttattgagc gtagcaaata atcggctatt
    25441 caaccaaagt atatgttcgt cgattgactg tgctggatca ccggttaaat tgaaatagac
    25501 atgatttgta ggattgaata ctgttttgcg gtcactgatc ccttggaccg tcactcgcca
    25561 tgtatcatcg ttcgtcagcg tataccgtac ttctacatct aaagttccag gaaatccgtt
    25621 ttccatatct gggctagtct gatagaaaat gaccgatgct tcgttttcac cattgatgac
    25681 tgtataatgc cactgcttgg cttcaaagct aggtgcacca ccatgtaaac aatgaccagt
    25741 gtccgggtct gtttttactt cgaacgtttc atcatctaat ttgaatgtgc cattcgtaat
    25801 ccgtccagcc atacgtccga ttgaagcacc gatataaggg tctttttcta gatattcttt
    25861 tgcttcatca aaacctaaga tcaactctcg tttttcatta tcgattattg tttgaaaatg
    25921 aacaacccgt gccccaagat cactgacaga caaagatgtc ccattttgat tcgtgatcgt
    25981 aatcaagtga tagttttcgc caaaattttg ttcattgatt tccatgttcc ttgcaccctc
    26041 ttttctttca tttgcactga ttccagtata catggaaacc gttttcttaa cagcttattc
    26101 cgtgagtaac gagaaaaaat ttactatatt ctaataaaag accgtgaaaa aaaggttgtg
    26161 acaaaaatat tgtcacaacc tctttttccg aacaaacggt gttcaaaagc ttgcatctgc
    26221 ggtaataagc ccaaccaaac gaaaaatatg aggagctatt tttgcttggc tgttcttatt
    26281 acttgatgca ggacagcttt ttcacaatct ctttttcaat acgattccag tatataacca
    26341 tgtcgtcagt acatagtaga tgaaatacaa tacgaagaat atcgtaaacg gaacccaaat
    26401 atctttgtat ggattttgca tgaatagacc aaacatttgt aatccgaata agacatggac
    26461 tacacctaag attcctggtg ctaagaataa cacgcctact tctctacgga tcgattgttt
    26521 aagcaacccg cgtcgtgttc ctatcttttc aagcatttca taacgagcaa tgtcactctt
    26581 gcttcctgac aagatcttga acatcaaaca gctagcaagc atggtcaaga aagctaaacc
    26641 taggaagaat cccataaatt caaaaccaga gaatagacca ttatacactt catacatttc
    26701 atatttctgg ttgctgtact ctactttttg caaagatgga ttgttcattt tattttgagc
    26761 aacaagtgtt tcgattggtt tcaggtttgc ttggaagtct tttacctgaa ctagctgtaa
    26821 agtggattgt ggcaagttca gttgatcaaa ttctgattga ctgactacct tgatttcttt
    26881 ttcttgctgt tccggcaatt catagcttcg aagttcctct tgtacagtca gttcattcat
    26941 ttttttagca ggaacttttt ctttcgtgat cttcggcggt gttccatctt catgttttat
    27001 catgatcaat ggttgagccg caaattcact tgcattatag taaatcgttc ccgcatcttc
    27061 tttttgagta taagaagcag aaagtgttgg tgaaagttct ttgatttttt gctgatcttc
    27121 tgcccgagga ttattcatca ctagatcata tccagaattg ttttctgtca agtctgcaat
    27181 ttcattttta aaacctaatc ctaccgtcaa agcgcccagt gccaaggcaa acagcatagc
    27241 gaccattgag agcatttgtg tatattcacg aatacgaaag tttaactgtg acaatgtgaa
    27301 attgttcaat tttttcaatg aaatctgttc tgatttcttt agtaatgata aaaagaaaat
    27361 gatcacagaa tggaaaatga aataagttcc taatacgatc gtaactaagg ctacccctat
    27421 accaaagact tgataggtcc ccacgtgttt catcatatag taaccgcagc ctagaaaagc
    27481 aattcccagt actacttcaa ggaaaaacaa gaatttatgc tgcttgattc ttgttggtgt
    27541 ctgatccgca cgaatcaatg ctaaaatcgg ttttttgaca atcgaaaaag cattgacaac
    27601 agcagctaat aaaaacaaaa cagcaaaaaa caaaacagtg atcaacaacc cgtgaatact
    27661 aaaaggtgta aaatgcgaaa tattgagatc taactgatcc actaataatc ggtggacgat
    27721 acttgtcaat cctacaccga cagctgaacc cactaaagta gctgcgatcc ctactaagaa
    27781 tgtttcagaa aaaatcatct gtgcgatttt tctgcctttt gctcccagca tcataaacat
    27841 tgcatagtct ttttgccgca tcgtcatcaa aaaggaattt gcatataaaa tataaacgaa
    27901 tgtgatgatc cctagtaaaa cagaaccgaa tcggaaaatg ataactgtag ccgcaacgat
    27961 cgtattactt ttcaaaaaag cttcgttgct tgccattgat tcaaacatgt aaaaaatagc
    28021 agatgccata actaatcctg agaataacac caagtagtct cttaaacggc tcttgatccc
    28081 agtaagtgat aatttccaaa gcatagtcaa tctcccccta ctgttccaag tttccaagaa
    28141 tagatagtat ttctcgatag aaggaatcac ggctttgatc tccacgtttc acttcttgat
    28201 ggataccgcc atccttgatg aataaaatac gttggcaata acttgcagaa aacggatcat
    28261 gcgtcacaag aagaatcgat acatgatctt ttgtattcaa ttcgtccatc gtatctaaaa
    28321 gatcccgggc acttttggaa tccagcgcac ctgtaggctc atcccctaat agaatcgttg
    28381 gttgtgtgat caatgcacgt gcggcggcga cccgttgttt ttgtccaccg gaaatctcag
    28441 aaggatagct ttccaaaata tgagagatgg ataaacgttc tgcaattttt tggacacgtt
    28501 gcttgatttc ttttggtctg acgccttgta aagaaagagg aacagcgata ttttcttgtg
    28561 ctgtcaagct ttctaataaa ttgaaatctt ggaaaataaa gccaatctca ttcgcacgaa
    28621 aatctgctag ttgatttcct tttaatgtgg tcacatcttt ttgattgatt cggatatgcc
    28681 catctgttgg tttatctaat gtggataaaa tattcaaaag tgtcgactta ccagaaccag
    28741 aagcgcccat gatcccaata aattcgcctt tttctacatc gaagctgatg ccgcttaatg
    28801 cttgcgtctt tttctcattg ttttttccgt atacttttgt tacattctgt acttcgacta
    28861 ttttgttcat ttctttccct accctttaat tagtttttcc agtgctgttt tcggcacgtc
    28921 ttttttttcg acttcttcat acgaagtgac acctttattt ttgttccagg tgatttttaa
    28981 atatgctcct ttacgaagcg gtcgagtcaa ttgtcccttg aagtccagtt ctttttcctt
    29041 accctctttc gtatagccag gcaggatata tctatagttg actgtttctt gtccagagtc
    29101 tgattttgaa actgtttttg taccatctgt cgtaatttgt acatagtatt cttctccacc
    29161 cataactacg taatctgcga ttttcacccc tgcaagtacc acaacgatga aaacaagtag
    29221 accgatcaga agttttttca ctttttcccc ttctctctta ctttatatac tgattataga
    29281 aaaaagcgaa caattctgcc ttcgaatcaa ctatcattct tctttcaatt ttgtcatttt
    29341 ctttaaaaca actaaaaaaa gagttcaaga caataatccg tcctaaactc cttcttccta
    29401 tcttacaaaa agtatgtcac ttacggtctt tcatgtcgcc tgtattgata acaggttgcg
    29461 ttcctttgtc tgtaatgatt gagacaagta aattattgat caattggact ttccgatcat
    29521 cggtgaaatt gatttcttgt ccatcttcga tttgctctaa ggccatttga gtgattgtga
    29581 cagctccttc aacaatggtt tgtcgagcag acaaaatggc ttttgcttgt tgacgttgga
    29641 gcatggcact agcgatttct gttgcatagg ctaaatggtt caagcgtgtt tcgattactt
    29701 cgacacctgc tactgctaaa cgctcttgca gctctttttg caactcgtca gacactgccg
    29761 tcgtgtttcc tcgaagcgtc aaatcattct cattgaatgt atcatacggg tattggctag
    29821 cgatatgccg aatggctgtc tcactttgga tttctacaaa atcttgataa tacgctacat
    29881 cgaataatgc ctttgctgta tcaacgactt taaagactac tactgctgag atttcgatgg
    29941 ggtttccatc caaatcattg acttttaaaa cagaactatt gaaattccga actttcaacg
    30001 aaacagtcat tttttgtgcc aaaggtatcg taaggaaaaa gccattttct cgaatcgtac
    30061 ctaaatattg tccaaaaaac aaaattactt ttgcttgatt aggactgact acagttgccg
    30121 aacttaacaa caataacgtg atcaaccaaa gaaaaatact taaaaaaagt gcccaaactt
    30181 tcgcttgcca catacctaaa tagaagaaaa acaatccaac taaagctaat acgaccaatc
    30241 cgacaatccc tgcataacca ttgatataaa atgctctttt ttctctcatg ctacttcctg
    30301 cgctccttta tccttcaata aatatctaat gttacttttt taattttttt ttcgaatacg
    30361 cgccattaca ccttctagtt ctaactcttt gtcttcagtg atgtaccaac cgcttgcctg
    30421 tgtccctccg gctgcctgcg gtaaataatc caattttgtt cgaacccagt gcttttcttc
    30481 tgtaaaatag tcttctacca agacctctag atccatccct gctgaaagag gcaaatcttc
    30541 ccgaacagat ttaaagataa atcgttcctt cttcccatca tacttcaata ttcctgtatc
    30601 gataatcggt aaagaataat aatccagctc tttcttcacc gtcaataaat gtttgaaaac
    30661 ttcatggtac acttgctcta attggcgacc ttctgaattg ttcgggtaat taaccatcac
    30721 tttttctttg tctggatcat agaaggtttg gcttagttga gtgatataag tatgtaatct
    30781 ttccatttct gtttttaact tttctctttg accttcttct accttcatat actctttcac
    30841 cctgttttct ttttagatat tttctttctt attttactat agttatgtgc gttaagcgat
    30901 gaaaaacagc aaagattgga aggatctttg ctgttttttc ttaaaatatc cagttgagta
    30961 caggatcaat ctgtttcatt gtcctcttga tccgtcgtaa tattgatcga tgtgtcttta
    31021 gcagtgatct ttagcttttt ctgtccttgt tctaacaaag tatttgttat tggctggtag
    31081 tctttttcaa tataagattc accagacgtt tggatagtat agccactgtt ttccatctca
    31141 tgcaaagata aattgccacc gtttaggatg acttttcctt ctttgctgat caaactgtct
    31201 tctagatgga agttgctgtt ttcacctgtg acgatcaagt tatccacatt ggcctcatct
    31261 aatcgaagtg agcctaattc atctttcgca gttattgttc ctcctgtaaa ttcattgaac
    31321 gatacccagc catcatgtgt cttagtattc acttttttga ttgatacttc gttgatatca
    31381 attgcactat tattcccttc gattgtcaat tcagataatt catgatctgc agggacaaca
    31441 atagtgattt gtgcacttgt gtcttctaaa ctaatgattg cacctgttga ttttttgact
    31501 gcattcacag tcaacacgcc atttttctcc tttatttctg gcttttctgt cttatcataa
    31561 gtcgttttta tctcaaaatc caatccgcgt atgaccgaaa ctgtttgatt ttctgcatgg
    31621 atgacgattt tgtttgcatc cttgattttt tttgtctcag taaccatatc gatcaaccga
    31681 ggaccattat cccaagtcag actcttttga gcgcctaatg tataagttgc acctgctagg
    31741 aggccaccag taagcatcag accaataccg gtaatcaaaa aatgtttcaa tctcaattta
    31801 tgtgtcctcc ttcaaacgcc cgctgaatgc agatctcttt ttgtaataaa acgtcgtccc
    31861 atccatttaa caaagttcat catgacataa aaaagccctt ttgttccttt cagcaacagt
    31921 ggcgaaataa atactgcgct tcctgtggcg aacagaccga caccgataaa gaataatcca
    31981 ctcagaatcg attgtccaaa cacactgatt cctgagatga tcatgaatag gccaacacct
    32041 aagatactga tacataaaac atagatacta aaaatgagcg ctagtaaagc aaccaatagt
    32101 cccccaataa ccaatataaa ccccagtgct agtggaatga ggacaggaga agcaaataaa
    32161 ccgataatga ttaaccaaat caggcgaatt tgtctttttg gatttttcac taactcgttt
    32221 tctgcttgtt cttcttctga aaagtaactg atttttaatt ttaaggcaaa ctgctttggt
    32281 gtaccatatt catcaatgat ctgatctaat gtttttccag aatctaaaaa atattcctca
    32341 taatactgga tcaaatcaaa acgatcgtct tcaggaattt cttgaagatg atccacaact
    32401 tcattcaaat attcattcat ttgtgctctc ctccaataac cggtctaaag aatctcgaaa
    32461 aacaatccat tcatcaataa tttcttctaa atgacgactt cccattggtg tcaacgagta
    32521 atatcgacgg tttctgcctt caaaaggttc atcataggtc tcaagatatc cttctttttt
    32581 caatcttctc aaaaccggat atagtgtaga ttcactgaca gagagacttt cttgtacgat
    32641 ttttgttaag gcatacccat acatatcttc cttgcttaat gctgctagca cagtaccatc
    32701 aagaagaaca gttggaatct ggatgtgcat tacgtcacct tctattctca agtgtaatat
    32761 tttatttcaa tctatattat acaacatata atatagtttc aaaatcagtt tgtcaaatac
    32821 aaaaaaactg actaaaagat ttgatccttt ttagtcagca aaaaatggct tctctttgat
    32881 ttttattata acagtggcat aatccaattt gtccacatcc agctcagtgg catgataacg
    32941 atcattgcag gtatcatgtc agctactggg aacattttca cgttgatcat tctaaaaccg
    33001 gtagcgacca tcaagaatcc gccgcaagct ttaaaatcta agatcatgtc tggtgtagtc
    33061 aatggataaa taaattttgc caataagaac aaaatataaa aaataatgaa ttggggaatt
    33121 gctatcacag aaacgacata ccctaaatta cacgcaaaaa tagctgctgt gaaaaaatca
    33181 aggacagatt tagaaatcaa aatcgtactg tctcctgtca tcccagaatc taaacttcca
    33241 tagatccccg taccacttgc acaaaataga acaatgaccg tcactaatgt gctgataaat
    33301 tcatcgtggg tcataccaag cttttcacta gggaagattt tagaaatagg tacttgcatc
    33361 agcgcagccc ccttatttat cccattacct aaatgaacaa tcaaaccaat cgctgttcct
    33421 atcactaaag caaaaataac agccggcata tttttcatcg gcgcaatgga atagattccc
    33481 atccccatcg aacacacacc aaaaaccatc gtcagctgtg ctttaaaatg ttcacttaat
    33541 ttatttccta ctaatccgcc aattagtccg ccaaacaaaa cggaacctac attgatcaaa
    33601 actccagttg gcatcaaaaa actcctctct ttttctacga agcgaaaaaa atcagaaaaa
    33661 tttctgtaat tattgctttc gctgcaagaa agattgtaac agacagagaa gattacacca
    33721 attcattttt tgtgttaaag tattctttat tggaataagt aggaggaata tgatggatat
    33781 ccataaattc gaagtttttc ttgatttagc cgaaacaatg aatttcacca aaactgctga
    33841 tcgacaattc accacccaag gaaatatttc taaacagatt ctttcgttag aaacagaatt
    33901 agacgtcaaa ttattcgaac gcgcccatag aaaaatcgaa ttgaccgaag ctggaagctt
    33961 attgctccca tatgtaaaaa atgtcgtaga acaataccat gctatgcaag aagtgcttga
    34021 aacctatacg aaagacaaca atctttcatt aaatatctta acgataccga caatgatcaa
    34081 ttataaaggt ttttccaaaa tcaccgagtt tttgaaacgc catcctgaat ttacagtcca
    34141 gttgaaagaa gtggaaagtg tcgaattaac ttacaatgac gccaatgaac atagtgacac
    34201 gatccacttt gctagaagtt tccaggcccc accaagttcg attgagtggt tgcctactga
    34261 aacagacgat tttgtagcag ttctacctaa aaatcatcct ttagctagta aaaagaaatt
    34321 ggatctaagc gaactgaaaa aggagaactt tttgctcctt ggccccacta cgaacttgta
    34381 tcaacctgta ctagatctgt gtcgcgaagc tggatttgaa ccaaaaatct cttataaagg
    34441 agcaagaatc gacttgatca tcaatatgat tgcaaatgac atggggattg ctattgttat
    34501 ggaaaaaaca gtcaagaatc tattaaaaga aaatactgtg attctcccta tttctccaac
    34561 aacagagagt tatcttgcat ttactcgtaa agtcggtgag cattctttgg cttcagatac
    34621 attttgggag tatttaaatc aagaagaaaa ctaagcccta cattttctgt ccatctatcc
    34681 acccaaaaaa agagagcgac gcatgacttt cgctctcttt cttgcatcat gtccttgttt
    34741 caatgacacc tctaacattt ctcgtagttc aataagaaag cagcgatttt tatgccaaaa
    34801 gaaccgtaag aacatttccc taatcacaaa aaaacgacta aaaaccccta aaaaccttca
    34861 atagaatttt taggggtttt attctttttc ctattgttta cttaaccgta tcattgctgt
    34921 cgttcctact ccttcgatac tgctataatc aatacgtcca tcatgcagtt ctagaatttt
    34981 tttaaccagt gaaagtccta gcccattccc agaaatttga cgacttgtat ctccttgata
    35041 aaatttttca aacattcgat ctctggtctc ttcattcatt cctacacctg aatcggtcac
    35101 ttccagtaca atctctgtgg ctgtctcaaa tagtttgata tgaatctgtc cattgatttg
    35161 attgtacttt atggcgttat ccatgatatt cagccacact tggtacaaaa actcttcatt
    35221 gcctgtgtaa tttactgcag caagttcgat atccaattct agctgttctt tctcccattt
    35281 aggctgaaaa aaaacgatta cttctctgat ttgttcatct aatcgatact ctttcttttc
    35341 cagttggata cgctggtttt ccagtttgtt caattttaag atgttctctg ttaattgtga
    35401 aagctgcgtg atactttcga taatccgata taaaaatatt tggcgttctt cgtcagagag
    35461 atttggggct tgtaacaatt gaacatatcc ttggattgtt gctagtggtg ttttgaattc
    35521 atgggataca ctggacacaa agtcattcct cagtgtctcg atactattga gttcttggac
    35581 cataacgttg aaatctttat acagttgctg tacttctgct actttctgct gttcatccat
    35641 tcgaatcgaa aaatctcccg ttgctaccag actcatattt gtccctagcg cactgattgg
    35701 atgcaggata cgccgcccaa caaaaatcga tattcctgta cccacgacca tactgaaccc
    35761 cgcaaagacg aataacgggt ataacgttgg ttttccagcc gattcagcag gcgagataga
    35821 atgtcgagac aataagaaaa taaaaccgag aaaacacagc agtgtcacta agatcgaaat
    35881 gaagacaata ctagcaaaat agatccataa ttgtgaaaat agcttttttt tcatctttaa
    35941 ataaccgcct tatagcctag tccccgtact gtgatgattt gaaaatcttc cgtcttttca
    36001 aaacgggtcc ttaaacgttt gatatgtaca tcgatcgttc gttcttctgt attcgtatct
    36061 aatccccaaa tatcatccat caactgttgg cgagtgaaga tcttgttggg ataagacagt
    36121 aatttataca gaagcagaaa ttctttttga ggcagaatca acgaatgctg atcttgtaac
    36181 actgtataag catctagctc taattttgtt tgtccgatta tcaagctact gctgtaattg
    36241 atctttgaac gtcgaagcaa tgcttccaca cgtaggatca tctcattgac atcgatgggt
    36301 tttgtcatat aatcatctgc tcctaatttg aacccgcgtt tcttatcctc aaaatcggcc
    36361 tttgccgtaa tcattagaac aggtgtgtca aatctggtgc tacgtaatga gtctagcaag
    36421 tcatagccat ccattttcgg catcatgata tctgtaatga ttaattctat acgtgtcgct
    36481 tctagttttt tcagcgcatc ctctccattc aatgcggtga tgactgaaaa gcccactcgt
    36541 tccaacacag aacggtaaag caactgtaat tgcggatcat cttctactac taaaatacga
    36601 ttcattgatc tccctctgtt ttttgaaaaa tttctagatt ttttcttgta gttcatcttc
    36661 agttaacatt tatttcgtat actacgatca ttgttctgat atacttatta gtataccgaa
    36721 tatttcaata cgggagaagg gtgattatgc ttcaattaaa agatataaaa aaatattata
    36781 aagtcggcga aaccaccacc aaagcattgg atggtgtgtc tgtcgctttc agacaaaaag
    36841 aattcgtagc gattttaggt cctagtggtt ctggtaaaac gactatgctg aatgtgattg
    36901 gtggactaga taattatgat tccggtgata tggtaatcaa cggaaagtct actaaagact
    36961 ttaaggacag tgactgggat gcctaccgaa acaactctat cggtttcatt tttcaaagtt
    37021 acaacttgat tggtcatcta ggcattattg aaaacgttga acttgggatg acattaagtg
    37081 gtgtttctaa agacgaaaaa cgaaaacggg cagaagatgc attgcaccga gtcggcttga
    37141 caaatcatat gcataaaaag ccaaatcagt tatctggtgg acaaatgcag cgagtggcca
    37201 tcgctcgagc actagccaat gacccagata tccttttgtg cgatgaacca accggagcgt
    37261 tagatacaga aacaagtatc cagatcatgg aattgatcca agagttatcg aaagaaaaat
    37321 tagtgatcat ggtcacacat aatcctgaac tggcccatca atacgcagat cgaatcattg
    37381 aattttctga tggtaagatc ttaacagatt cccatcctca catcgaacga ccaaaagatg
    37441 atcaatttaa cttacgcaga acaaagatga gtttttggac agcgttaaag ctttccttca
    37501 acaatatccg aacaaaaaaa ggacgtactt tcttgacctc ctttgcttcc agtattggga
    37561 tcatcggaat cgcgatcgtt ctgtcccttt cttctggatt ccaaaaacaa atcgataaca
    37621 cgcaagctga aacgatggcg aaatttccga ttactatttc taaggtaacg actaaccaga
    37681 ctcgtgacga tgccggattg ggtgcaagca aagcggatta tccagacagc aaaacgatta
    37741 ctgcaaaagt cagcgaagaa gataaagcac agcacacgaa taagattgat caaacatacg
    37801 tcgattacgt aacagacatc gatcctaact taagtaataa tatcggattt acccgtacta
    37861 caggaatcaa tcttttacgt gatgtaaatg gcaaagtaca gcctgtcagc ttctcgaatc
    37921 aaaatcctga tacggaatct ctttctttat ctagtacgat gtcagcaatg acaggtgtgg
    37981 gagtgtcaag tttccctaca cagcttgaca ctagcaaaga aaacttttta aaagacaatt
    38041 attctttact tgccggaagt tatccagcct cagcaacaga tgtcgtgctg atcgtagacg
    38101 gcaataataa tactaatatc aacgcattga agaatcttgg atttgatgtg aaagaagacg
    38161 aaaaacttga ttttgatgat atcgttggta caacattcaa attagtaaac aacaatactt
    38221 actatacaaa acttccaact gggaatttta tccctaatac agattatgat gcgatgtatc
    38281 agaatgcttc tgatgaattg aagatctcgg ggatcctacg cgtgaaatct tcttctacaa
    38341 tgaatctctt atctccagga atcgcttata gcgatcaatt aacgactcaa atcgtcaacg
    38401 aaaacaaaga atcagaaatc gtcaaagctc aaagagacag tggtgtaaat gtcttaacta
    38461 ctgaaaaagt tgacgaaaat gccaaacaaa cattgcttag ttatttaggt ggagacagct
    38521 tgccttcaag tatcatgatt tatccgaaca attttgaaga caaagaaaag atcttggatt
    38581 atctagatga ttacaataaa ggcaaatcag atgaagataa aatcatttat actgatttag
    38641 ctggaacgat gactgaactg actggcggat tgatggatgc tatcacttat gtccttattg
    38701 cttttgcagg gatctcttta gtaacaagta tgatcatgat cagtatcatt acttatacat
    38761 ctgtgattga acggacaaaa gaaatcggtg tactaaaagc tttaggtgca cgtaaaaaag
    38821 acatcactcg cgtatttgac gcagaaacgt gtattttagg tatttcttct gggatattag
    38881 gtgtgtttat tgcatggcta gctacgttcc ctatcaatag catcttatac aacatgacgg
    38941 atctaaaaaa tgttgctcaa ctaaatcctg tccatgcgat tattctagtg atcgtctcta
    39001 ctatattgac gatgcttggc ggtcatcttc ctgcacgaat ggcagcgaaa aaagacgccg
    39061 ctatcgcttt gcgtgcagaa taaggttgtg acaaccacat ttagatttga gtcataagag
    39121 cgctgaaata gtagaaaaca gcaagaaacg aggaagtaat atgttccttg tttcgcgctg
    39181 ttttttttaa ttatttttta taattctggt atacttttct ttcctttgta ccacaactct
    39241 tctggcgtat acctgtcttt tccatttatg aatactgttc catctgcttc aatcgttact
    39301 tcagtcccaa tccacatatt acgagaaagt gcgagaagat tttttctttt tcctcttcct
    39361 ttttttccaa attgcaaggc atcggttatc tcttctgtcc aatgagattt cacgtatttt
    39421 acttctccgt tttcaaatgt aactttcaaa atattcgcta tattttccac ttttactgct
    39481 ttaggaaatg actcctccat ttttttcact ttccttcctc caacactaag tatgcgcttt
    39541 cttttagtga taagtatatc atagtttatt ctattatcaa aaaaacgcat ccattccatt
    39601 gtagaatact ttaccgaaca agttgaattg ttttcctctt tttcaccttt tactatagat
    39661 aggtaaagac aactaaggag gaatctctca tgacagatca accgtatgat ttaagaaaag
    39721 tcatcgaaga actaaaaaca cttccaggtc aatatcacga aacagatatt gaaatcgatc
    39781 ctgaagcaga attatctggt gtttaccgtt atatcggtgc tggcggtaca gtaaaacgtc
    39841 cgactcaaga aggaccagct atgactttca acaatatcaa aggtttcccc ggcacacgtg
    39901 tgaacatcgg aacaatggct agtcgtaaac gtgtaggtga aattttgcac catgattatc
    39961 gaacacttgg tcgtttcttg aaagacgctg tagaaaatcc aatcaaacct gtgaaagtag
    40021 acaaagcaga cgctccagca caagaagtcg ttcatctagc taccgatgca gacttcgata
    40081 ttcgcaacat tttgcctgca cctacaaata ctcctgaaga tgctggtcca tacatcacaa
    40141 tgggtgtagt cttaggatca gatccagaaa aaacaatgag cgatgtaacg atccatcgta
    40201 tggttctaga agataaagat acgatcggta tgtacatcat gcctggcgga cgccacattg
    40261 gtcacttcca aaaacaattc gaagccttga ataaaccaat gcctatcact atcaatattg
    40321 gtttagatcc agcaattgcc attggcacga ctttcgaacc acctaccact ccacttggct
    40381 acaatgaact atggtgtgca ggagctttgc gtaacgaacc tattcaaatc gttgatggta
    40441 tagcagtcga tgaagtgggt attgctcgtg ctgaattcat cattgaagca gaaatcatgc
    40501 caaatgaaac aatgcaagaa gatatcaaca cgaactcagg aaaagcaatg ccagaatttc
    40561 ctggctatac tggggatgct aatccagctg tacaagtagt gaaagtcaaa gcagttactc
    40621 accgaaaaga caatccaatc atgcaaacaa cgattggtcc cagtgaagaa cacgtatcta
    40681 tggccggtat tcctacagaa gcaagtattc ttgccttggt cgataaagct attccaggaa
    40741 aagtagtaaa tgtctacaat cctcctgcag gtggtggtaa attgatgacc atcatgcaaa
    40801 tccgtaaaga aaacgaagcg gatgaaggta ttcagcgtca agcagctatt cttgctttat
    40861 cagccttcaa agaattgaag acagtcattt tagtcgatga agatgttgat attttcgaca
    40921 tgaacgatgt agtttggact ttcaacactc gtttccaagg agataaagat atcatcgtga
    40981 tcccaggaat gaggaaccat ccattggacc catcagaatt accagaatat aatcctggct
    41041 ctattcgttt ccgtgggata agtgcaaaaa ctatctttga cggtacagtt cctttcgata
    41101 tgaaagaaca ttttgtacgt gcaaaattcc aagaagtaga agattgggaa aaatatttga
    41161 aataatgaat gatctgcaac tgttttgaca tcataaaaag gagtgcggaa aatggaacaa
    41221 ttggcacaac ggaagaaaaa aatcgtgatt ggtgtaagtg gcgcttcagg caccatttat
    41281 gccatcaacc tagtgaagaa actaaaagaa tatccgatga tagaaacaca cggaatcatc
    41341 agtccatggg caaaacagaa cttaaagctg gaatccgact tatcactaac tgaattcgaa
    41401 agcctgtttg attaccatta ttcaaacaaa gatttaggtg ctgctgtcgc cagcggatca
    41461 tttttgaccg atggaatgat catcgtaccg gcaagtatga aaacagtcgc aggtatctct
    41521 gttggtttag gcgacaacct gattgcacgt gctgctgatg taacaatgaa ggagcaacga
    41581 aagttagtca ttgttcctcg tgaaacgcca ttaaatgcca ttcacttgga aaacttgact
    41641 aaattagcac gactaggtgt gcaaatcata ccaccaattc ctgcttttta taatcagcct
    41701 caaacgatcc aagatcttgt taatcaccat acaatgaaac ttttggatca atttgaaatt
    41761 gtaaatgagc attcaaaacg ttggcagggt gattaatatg gaaaaagaaa cgatagttga
    41821 agtaaccaaa ctaggtttca cgatccaagc tgctgttaaa gtaatcggta aagacttatt
    41881 gatcacttta acaggcggag atacaccaca tatcggaaca gtcactacat tttctcgtga
    41941 tacagagatg caaactgtac gtttccctag ccacgatggt cggttccata aagacgatgt
    42001 cttagccgaa aaaatagctg tgatcatcaa aactgccgtt ccgggaagtt gtacgatcac
    42061 ttccggtatc catgttgacc atatctccaa tgcacaaatc aacgcctctt ttccgatgtc
    42121 agaagagtta ggttacaaga ttttagattg gttggaaaaa atagatttcc atatcaaaga
    42181 accagtttat tataaagatg gtgaaaaacc attatgaaag aagaggttgt gaaatcagcg
    42241 ttatgacctg agtatgagag aaaaaaatgg aaaaatagct tctcatattt tgccgttttt
    42301 gagctaatac cgagggacag ctgattgaac gccgtttatc aagaggttgt gataaagccg
    42361 cttcgtttcg agtagtaaga aaaattttgt aaacatgggt ttccacattt ttgcataaat
    42421 ttgacttact accgagaaac tggcttttga acaccgttta tcaagaggtt gtgacacgtc
    42481 ttttgtcaca acctctttta tttttgcttc actgtaactg ctacaatatc accgatttct
    42541 tttccgatgt ttttacgaat atcttttcgt agtccaagga tgtagcagac tgaaccatct
    42601 ggatttttca cgcccatatt cacgatactg ccttcatacg gcacattgtc aaaagttgct
    42661 tggactttaa ctcttccagc accgaattct tttcttatat catacggaaa aataatatat
    42721 gccccgccct tgccgacttc ggaagcatag atttttgatt caaaagaata aatttccctc
    42781 attttatttc tcctattttt tacatctagt atagcatagc aaaaaaaaga aggaactgtg
    42841 acaaaagtct tgtcgcagtt ccttttttcg aataaacggt gttctcctgc caactcctcg
    42901 gtcacaagtc acaatagtca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    42961 ctcagagtta aacggcaggc tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    43021 tacggcacta agcccaatca gatgaaaaat atggagtgct atttttatct gattgttctt
    43081 actgcttgat gcagagcagc tttttcacaa cctcctacat ggtgttcaaa atattattcg
    43141 ccttttgctt ctttcactaa ttttgcgaat gcttgttgtt ggaattcagc agctaaggct
    43201 ttcgtacgtt gttcttgctc tgctttgctc atttttggat tttcagtcat tgcttttacc
    43261 ataccgtctt gaacataagc ttttgcttca ctttccatcg tactttgctg tttcttcact
    43321 aattcagcta acttggctgc ttggtccgta cttaattcta cccatgtctc tgggattttg
    43381 aatgtattca ctcggctgtc atattcgtga ttgttatcag caatattgaa ccagaattta
    43441 tacatgtcgt ttttgtattc ccaataagta gttttcgtca ctttttcagc atcgccgtct
    43501 gtcttttcta ctttatttgt ttcgttatcc gtaccagttg cttttggttt ttcttgtttt
    43561 tcatttgttc ggtaaagata cactttttct tttccattcc ctaaagcttt atacaaaagc
    43621 atatctgctc cttgggaatc agcactggaa acaatttttt ctgtttttgt ttccgttact
    43681 ttttccattc caaagtgatt ggacaaatta gcgacgatca aacttagtga agcgacgaag
    43741 attagtccga aaatcaaaga taaaaaggtt tgccatgttt ttttagcgaa cacgttcgtt
    43801 aaagcaaaag caagcaagct gacaatcaaa actccaataa tcatgcatta tcccccgctt
    43861 tcattagaac aggttctttt ttcttcaata aaaaggcaat aataaagcca agaatcccaa
    43921 agatgatcgc tacgataaac gcggcatggt aacctgacag tgtggcattg attgcctgat
    43981 ctttataagc taatggcgta ttatccaata cagatttgct tgggatattt gcttttgtca
    44041 cattcgtcaa cacactgatc aaaatagctg taccaatcga gcttgcgatt tgacggaatg
    44101 tattatttac cgctgtccca tgactgatca agttatttgg caatgcattc ataccagaag
    44161 tcgttaccgg catcatgacc atggagatcc caaacatacg gatcgcataa aggatcacga
    44221 tatataaaac tggagtcgtt tttgtcaaga aagcaaatgg tgcagttgcc gctgtcaaaa
    44281 tgaacatccc tgtgatggct aaacgtttcg cgccgtattt atcgaaaatc gcacccgtca
    44341 caggcatcat agcccccatg atcaatgcac ccggcaacaa catcaagcca gaatggaatg
    44401 ctgattctcc acgaatattt tgaatataca atggtacgac catttctgcc cctaccatcg
    44461 ccatatttgt tacaccagct aaaacagctg caatcgtgaa tgtagatgat ttgaatacac
    44521 gaagttccaa gaatggatgt tccaaatgta attgacgcca tgagaacaag gcgatgaaaa
    44581 tcacaccaac aactaagaaa ccaattactt tcaagcttcc ccagccgtca tctcctacac
    44641 tagaaaatcc gtaaagtaaa ctaccgaatc caatcgttga taaaatagcg gataaaatat
    44701 caatgctagg atttgacaat ggaataacac ttttcattag gaagaatgct aaaaccaaga
    44761 caagaacaac gattggcagt accataccaa acaagtcacg ccaatggtag ttatcaatga
    44821 tataaccaga taatgtaggg cctaaagcag gagcaagtcc aataacgatc cctgacatcc
    44881 ccattgctga tcctcttttt tctggcggga aaatagatag catgatattt tgcaataatg
    44941 gcatcgatac tcccacaccg caagcttgga tcaaacgacc gattagtaat gtactaaaat
    45001 taggtgctgt aaagcaagta atcgttccga tcaaaaagat cgtcatggca gaaagatata
    45061 aatttcttga actgaattta ttgatcagcc atgctgttat tgggatcatg atcccgttga
    45121 ctagcaagaa accagtcgtt aaccattgca catcagacgc tgaaatatcg aaagctttca
    45181 tcaatgtagg aaaagctgtc gtcaataagg tctgattcaa aacggtacaa aatgtaccaa
    45241 ttaataagac aacaactaaa agtgtccgat tatatggttt tccgtaaata tctacgggtt
    45301 ggttgttact catatttctc cttctttctg aattttttct gaaactacta gaatacttta
    45361 gtcctaaatt cgctatttgt caacctactt gacacataag ttaatacgga tataattaag
    45421 ggaagcaaga ggacaaaagg agtgacaaaa atggttgggg cttcccttaa acgtttgttg
    45481 gaaaatgatc aattggttgt tggcatcagt gaattaagtg atatcgtagg tgtgtctcca
    45541 cgtcaattaa ggtattggga acaaaaggga ttcattcgtt cgatcgaagc tgatgcgaat
    45601 tgtcctagaa aatatcgttt acctacagtc ataaaagtag aactgatcaa aaaatattta
    45661 gatgacggat ttactttgtc aaaagctgca gaaaaggcag aaatccgcca aaagaaaatg
    45721 caccatgtcc gtaaagtttt ttcaaaagca atcaaagata tcgaattgat cgacgaacgc
    45781 tatacgattt tacaaatcgg taacttcaaa gaagagaatg ctaagatgta catcattcat
    45841 gacaacgaga cagacgaatt aacttacaag ctccttcctg tcgatgaaaa agctgactac
    45901 gaacagttgt taaaagaact ctaaacgcca ctaatctcct aagtaccaag ctttttaata
    45961 agtagatgct acataaatac ttgaaaaaag gcctgtttga acataagaat atccgaacgg
    46021 tgatctgcgt ttctttcata tagaaaggaa ttctccatca ctgttcggat atttttttat
    46081 ttttttcggt tactttcttc taatacatct gttagccaat ttgcatattc tgtttcccga
    46141 ttgatcgcat gcgctaaaat caggtagtgt ccgtaatttt catgtttttc tttttcagta
    46201 ggaaagatcg ttttttttcg ctctaataga tgaagtcgtt tttcttcatg aaggtcacgc
    46261 tgctgctgaa taatttctac taatgccgga tcattcacgt ctttgacgaa atacaatttc
    46321 agaacaaatt cgtctcgatt aaccggtagt tcattggtgg gtgtatgaat ccactctgcc
    46381 aattgttcct tcccttcttc tgttaattca taaactttct tttccaattt ttcacctgtg
    46441 atctctactt gatgctgaat gattccttgc tcttccattt ttgcaagttc aggataaatc
    46501 tgactatgtt ttgcttgcca aaattctcct atttcatttt tgaacgattg attcaattca
    46561 tatcctgtca tcttcttctt acttaataac cctaacaaaa tatatcgaag tgtatttttt
    46621 cttgccatca gttttccctc cttgacaaat ctttaaatcc attctatcat aaatgagtaa
    46681 tcgaaaacat gtagtttatt acatgtttta atttataagg agatgcttaa acatgaaaga
    46741 atttaaagaa ttggaagatt ttattggaac tcactttatc tatacgtatg acaatggttg
    46801 ggaatatgaa tggtatgcca aaaatgacca tactgttgat taccgtatcc atggcggaat
    46861 ggtcaaaggc cgttgggtaa aagaccaaga agctgatatc gttaaattga cggacggcgt
    46921 attcaaaatc acttggacag agccaacagg gacagacgtg gcattggact ttatgccaaa
    46981 tgaaaagaaa cttcacggaa tgatcttttt ccctaaatgg gttgaagaac atccagaaat
    47041 cactgttact ttccaaaacg aacatatcgc agaaatggaa gcagctcgtg aaaaatatga
    47101 aacttatcca aaattacttg ttcctgaatt tgctacgatc acttatatgg gtgatgcagg
    47161 tatagacaat gaagatgtta ttagcgaaga accatacgat ggaatgccag atgatatccg
    47221 tagcggaaaa tacttcgatg aaaattataa acgagtaaaa taaggttgtg aaagagaggt
    47281 tgtgaaatca gcgttctgac ctgagtatta gagaaagaaa cggaaaaata gttcctcatg
    47341 ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcaagagg
    47401 ttgtgaaatc agcgttatgc tttgagtaat aagaacgcta gttagaaaat agcttttcat
    47461 attttctagg ctagtgggct tattaccgag aagcagccgt ttgaacaccg tttatcaaga
    47521 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaatggaaaa atagttcctc
    47581 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaaa
    47641 gattgtaaca actgcatatt cacacagtta aattagtgac ttcaaaatga aataatgctc
    47701 ccagcaaaaa attaggctgg gagcattatt tcgttattct gttcatcaaa aatcagcgtt
    47761 ttgattccat ttcatcacaa gttcctcctc atttactgct tcatcttttt gctgtgtttg
    47821 aattgtaaaa ccttgtgaaa gatagaaatg aatagctgat tgattttttt gatagactgt
    47881 caaagtcagt gttttattgg tttcttttat ttttttcatt agttgcgtac cgattctttg
    47941 ttgccggtat tcccttttca caaagattcc tgcgataaac tgatcaatca ccccagcaaa
    48001 accaataatt tcttgatcta tttctgctaa gtaaatcact gcatctggta aagctttcct
    48061 aacagattct acttgattct cccaataagt tgatggaatg aattcatgag aatccaagtt
    48121 agcatgcagc caaatcgata gaacagattc cagttctttg gcagacaatt ttgttgttgt
    48181 gcggatcatt ttttctctcc ttacttattc attgatttta aaataaaaac catttttttg
    48241 aagctctttt acaagttttc gcccctcaaa aaagtatatc ttctcttgtt ttacttttct
    48301 aataccaaaa gaaacagcaa ctttaggatc atcagctgaa acgattcgat aagttccacc
    48361 aggcaattga tccagcttga ctttcccatg atggtcgctt tgtactgaaa acggcacacc
    48421 atttctagta agtgtttttt tcttatgttt caaaatgaat gaactaccat ttaccggttt
    48481 ccctgacgaa tcactaataa taaatgaaaa atcaagcact tttttactta atctatactg
    48541 atagattagt cgtccgataa acactaacag tatgactaca gctattatcg ttcctgcaag
    48601 aataaaccat tgccgcatgg ttctttcctg atttcctttt ccttcttctt tttttaccga
    48661 ttcgttataa ggaatccgat gtcctgtcac tagcaaacga tgcgtgttga tcatatacgg
    48721 cgtacatgta agcaaggtaa ctaagtcttc tccttctgaa atcgttagaa cagacgtgtc
    48781 atctggcagt accacttgta tatttttaac ttgataagcc aatttctccc ccaaaacagt
    48841 caaaataaac gtgtctccgt tttctaaacg atctaattcg gtaaataacc gtcgattagg
    48901 taaaccgctg tgagcagaga tcactgaatg cgtcgaattt ccaccaagcg ggaaagaggt
    48961 tccttgcaag acgcctgctc cattttcaag gatcctacca ttgagccgat cataaagtga
    49021 aatatttaat tggatcttag gtatattgat acttccgatc agccattcct ctaattggct
    49081 ggatgttgta ctttcatttc tgttcttttc attgaaagga tcagtaccag gattgaatcc
    49141 ttggtcagtt aatcgttggt ttttctcctc cattttcttt cgctgcactt caatttcagc
    49201 tgcggtccgt ttttgtactt gttcgatacg tttttgatca ataaaactat ttaaagaatc
    49261 cacataaaat ggatataaag cgatcagtaa ccctacgacg atacatatta ataaaaacaa
    49321 acgaaacaaa agtcgacgag actgtttttg cttactctgc ttcacatgtg tcctcccttg
    49381 caagtttttt tgttctttct tttattatat gggttcagga aaaaaataga aaatgaaagc
    49441 cgatgaggtt gtgaaaaagc tgtcctgcat caagtaataa gtacagccca aaaaaatagc
    49501 tactcatatt ttttgttggg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    49561 taaggaagag gttgtgaaaa ctgcgttatg ctttgagtaa taagagcgct agtcagaaaa
    49621 tagcttctca tattttctag gctagtgagc ttattaccga gaagcagctg attgaacacc
    49681 gtttaccgag aggtcatgaa aaagctactt tgtttcgagt attaagaaaa attctgtttc
    49741 aaaacaagga gatgcaacaa aacttatgtc gcatcctctc tccttagaac tttcacttat
    49801 gtttttcttg taaaattcat aaatattttc atgaattttc taatttcttg cctacctctc
    49861 gcataggata tatgatgaat ttatctaaag tttgcaaagg agttcgacaa ttgaaaaaat
    49921 atggcccatt tttagcaatt tttttcattg ctttggcatt tctcatgcct catttattca
    49981 cacataaact aattttgggt gctgatgccc cattccatta caatcggttc tatgatgccg
    50041 cgcaacaaat taaatatggg gatttccatt attttccttc cctttatgga ttccagcaaa
    50101 gtggcaggat cgtgaatgcc gtttacggcc catttttcgc ttatttacaa ggattgatcg
    50161 tgttgcttgc aggtagctgg tttcattatc agattctttc aaatttcatt ctttatctta
    50221 tttcaggatt ttctatgctg attttactga gatatgtcaa tgttcgaaat tgggtaagtg
    50281 taccagtagc cgttttattc atgagtacgt attctattca atactggtct atcaaccaag
    50341 gattcactag ctgggggact tgtttttatc ctttatgtat gatccctatt gctgatttcg
    50401 tccttcataa aaagttccct gttatcaaag tcgctgtctc agtgggactt atgacacaga
    50461 tccatttact gtcttcgatc atgttagctt tgatgtatat ccctttttat ctttattact
    50521 tttttaacca gactgaaaaa ggaaaagcac tgcttcaact gattaagtcg gtcttgcttt
    50581 attttgttct gacagcaaat atttgggcag gattagctat tgtttacagt gggaataaga
    50641 ttcttcctcc ctttgttaac agagaaatga gtcaaaaggc gatagatctg gatggcagtt
    50701 attggctcca ttaccctaag gcctttccta tattgatatt atcaggtttt ttgcttttta
    50761 ttttctttca acaaaagaac accttattcc aaaaaatcgt gttgtacaca agtcttgttt
    50821 tcttttttct ttctactagc ttgattccat ggacgacatt gatcgaaaaa aatgtgcctt
    50881 ttattagttt gatccagttt ccatttcgat ttttcgtgcc atttacgcta ttgcttctac
    50941 tttaccttgc cctttcactg aaccagtggt ctgaacaaaa gtggttccgt cttttaagta
    51001 tatttggcat tcttattttt tcagcacaga cgatccagaa cttataccag catctggaaa
    51061 agtgggaaaa tgaaacattt gtcagtcgac atacttatct attcgatacg ccggaagaag
    51121 caaggaaaac attcttcagc aaagatatgt ctaccagctt gttcgttttc caaaaaacca
    51181 cacctgacta tctgccaatc tataaggaga caaaagacaa taaatacgac cggtataacg
    51241 aagaaatact agaaaacgaa acaactttta taaaaacaca tccaggcggt cttttgacaa
    51301 tcaagtggaa aaacactggg gacaaaactg ttcatatccc tgttatcgtc tacgatcgga
    51361 ctgtgctaca gcaaaatgga aaaacattga ctgattatga agtaacagat atcgggaccc
    51421 caatcgtgaa gcagcaaaaa ggaatcaacg agctcacgct tcattatcaa acgccaatct
    51481 atttctattt cattctttct cttactttga ttggctggtt taccttgcta tgtttattta
    51541 tttatcatag atacaagctt ttacgtgctt aggcaaaagg gataaataca tccgatcaaa
    51601 aaactctcca aacggtataa tcgtttggag agttttattt taatcagttt ttttatgtta
    51661 tatgttatat tttttattcg ttcacatcta attcgatttc caccccgtag tgatcactga
    51721 tgattggact atcgatccca tcaaaaatcc gttgatattt tttaacctgc attccttcag
    51781 ggacgaaaat aaaatctata cgcaattttt ctgtattttg ttcccaacca tctattttct
    51841 tttccacagt agctgctttt cctttttcat tcgcgacaac atacgaatct tgaagaagaa
    51901 ggcaactttc tcttacagta taataacctt gagagtctac tggattattg aaatcaccta
    51961 ggaaaaaaag tggtacgcga gtttccaaaa agtagttttc caagtttttc cactcaaaat
    52021 aaaagccact ttttttatct gtccaccaag aaaagtggca gctcgagaca aaaatgtcct
    52081 gatcatctat ctttgttttc ccaatcaaga tcttgcgtgt ctgataatct tctttctttt
    52141 gaaattggga gacggtaagg acctgacttt ccaatgtttt tttcgtaagc agtgcatttc
    52201 cttcttcata tttatcataa ccgatgtggc tcatttccca actccaaaaa tactcttttc
    52261 cgtgttcctt taaaagctga actaaacggt aggcaaaatt atctttacgg ataggtactt
    52321 gattctttac cggacagaac ttcattagct tcgcaggttc tacttcttca gattccagta
    52381 attggttgat ttcttgtaaa gcaatcactt cataatcttc taacaggatc ttatctgcta
    52441 attcttgcaa ttttttctct ggttctggct caagccagct atgcgtgttg agtgtcatga
    52501 atttcattgg ttttctcctt gtcttcattt cgaaacaaaa gggttgtgac aaaatttttg
    52561 tcacaacctc tccagcaaat agtgttctcc tgctaactcc tcagtaataa gctcaatgga
    52621 ccaggagcta ttttttgttt ggctgttttt atcacttggt gcagggcagc tttttcacga
    52681 cctctccact aaacagtatt caaactgctg cttctcggta ataagcccaa ccaaacgaaa
    52741 aataggagga gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    52801 caacctctcc ttacatttta atggagccga tcactttacc agctgtcact gtacctaatt
    52861 gcttgatcac tacttcttct actttatcgt ttgtaaatac gatcatgatc gttggatctt
    52921 tttcttcgtt ttggatcaca tcaagatcca tctcagctaa tttcgttcct gttcctactt
    52981 cttggccttc tttgacagca atctcaaaag ctggctgatt catttggact gtatcgattc
    53041 ccatatggat caaaacttcg ataccatctt tcgtttccag cccaattgca tgtttgctag
    53101 ggaaaatact tgtcactttt ccagcgatcg gcgcaacaac ttctcttgat gttggaataa
    53161 cagcaaagcc gtcacccatc attttttgtg agaataccgg atcttttact tcagataaag
    53221 gaatcacttc tccagaagct gcggaaacta gttcttgttc tatacctaag tattgcttac
    53281 tacctgcttg tccatttcct gtagaaacca tttctggctc tggaatctct gctcctgatt
    53341 gcagcagatc ttcgatatct gatttcaaga catctgcttt tggtccataa actgcttgga
    53401 ctccattgtc tttgacgatc aatcccatcg cccctgctcg tttccaggct tcttcagtcc
    53461 ctactttttc tcgatcattt acacttacgc gtaaccgagt catacaagca tcgacatctt
    53521 tgatattttc ttttcctccc agcaaatgaa tgatctggac cacttgctga tctgctgttc
    53581 ctgttccgct gttagataca acggtttctt cactgtcatt gtcgtaattt ccgttacgtc
    53641 ccggtgtagc aaagttgaat tttttgatca agaaatttgc taagaaatac gtaacaacac
    53701 caaaagcgat cacacacaac acgaagttga taagatctcc acctagaccc gctttgatag
    53761 ctaacggtgt ccgagtcagc aattcgatat tcccaaatga atggacccgc aatggcaaga
    53821 tatctgccat cgcaaatgca gccccttgga tcactgaata aatgacatat aatggcacag
    53881 cagcaaacat aaacatgaac tccaatggtt ctgttacacc tgttagaaag acagccaacg
    53941 ctgctgaaaa atacattgat ttatatcttg ctttcttatc aggatcgaca ttacgataca
    54001 tcgcaaaagc cattcccatc aagatacctg aagaaccaat catttgtcct actttgaaac
    54061 gagccggcgt ccagttatgc aacacaaact catattgcga catatcccct gcacctttaa
    54121 gattcactaa atcagtggcc caagccaacc ataacggatc ttgcccaaat acttgtgtcc
    54181 ccgcctgtgc gcctgataaa atctcatacg atccgccaag ctgtgtatag ttgataggaa
    54241 tcgtcagcat atgatgcaat ccaaatggca atagtaaacg ttctaatgtt ccaaataaaa
    54301 acggtgctag aatcggtgcg ctttcttgtg attctgcaat ccataaacca aaattattga
    54361 ttccagcctg tacgtttggc cacaccacag caagaatcaa tgccacgatt gttgaccata
    54421 agataacaac aaacggaaca aagcgctttc cattgaaaaa tgacaaagca tccggcaatt
    54481 ttctataatt atagtactta ttataagcca ttgccccgac aaatccagcg atgatcccta
    54541 cgaatactcc catattcaaa gctggtgctt ccaatacact tgtaaaaaat cctttaacca
    54601 tgatcttcgt accgaacaga gtgtgggtaa atgcatcttc attcgcaagc atgtcacttg
    54661 tgacaccaaa gatagcccca gtgatccgat tgattaaaat aaatgagatc ccagctgcaa
    54721 atgctccgcc tgctctttct tttgcccaac ttcctcctat tgctaaagca aataaaatat
    54781 gaagattacc aataattgcc caaccaatat tttcaattac tccgcccgtt gtgacaagcg
    54841 gcgtccaatc aggattgatc aagggaattg atttccctat actgatcatc aacccagctg
    54901 ccggcataac tgctaccaca accattaaag cctttccaaa tttttgccaa aactcaaaac
    54961 taaatatctt tttcattccg tttagctcct ttacgcaatc ggtttcgtta aacgtattat
    55021 agcgtgcgtt ttcttttttg taaatgcttt attttttatt tttctgtcta taccattcta
    55081 taaaacctat aatacgtgtg ttcccttcta taattttctc ctaaaaattt atttttttaa
    55141 gcttttttct atgaaatatt ttcttttgtc tattgacttg tgcaatcggt tgcgtttatt
    55201 atgaacgtat aaaacagctc attaggagga gaaaaatgaa acagatcaaa cgactatttg
    55261 ctattgatcc atggaaaatc agcacgacac aattagataa aaaaaatctt cgactgcaag
    55321 aatctttaac aagtattgga aacggttata tggggatgcg cgggaacttt gaagaaactt
    55381 actcagggga ccaccatcaa ggtacttatt tggcgggtgt ctggtatccc gataagacac
    55441 gtgtcggctg gtggaaaaat ggttatccag aatactttgg aaaagtgatc aacgccttga
    55501 actttatcgc gatagatatt tcgatcaacg atcagctgat cgacttggct tctttggaac
    55561 cagaagactt ttactgggaa ttagatatga aaaatggtgt attatctcgt acattcacaa
    55621 tcactacttc tactaataaa gtacgcattt ccttcgaacg ttttctgagc attgtcaaaa
    55681 aagaatcagc ttatatctat ttaactatag aaatgcttga aggtagcgga acagtcaaac
    55741 tcgtatcaaa attggatgga aatgttcaaa acgaagacag caactatgat gaacattttt
    55801 ggaatgagat tgatcgtgga actatagaag aaatcggcta tttgacgact aaaactattc
    55861 caaacaactt tgggattgaa caatttactg ttactgcagc aatgcgccat ttcgttaatg
    55921 gaaaatcagt catccctgac tatcacgaag atactttagc attacatggt tgcttgtctg
    55981 cagaattatc tcaaggtgag acatggtcag tggaaaaaga aattatcgtt cttactagta
    56041 gagatattcc agaagcgcag caagcagaaa aagctgtctc agaattaacg aaccagacaa
    56101 acaattatat ccaagcaaaa gaagagcaaa cagcggcttg ggcagaacga tggaagcttg
    56161 ccgatgtggt catcgaagga gacgaagaag cacaacaagg tattcgattc aatttgttcc
    56221 aactattctc tacttactac ggcgaagatg aacgattgaa tatcggcccc aaaggattca
    56281 caggagaaaa atacggcgga gccacttact gggatacaga agcgtatgct gtccctcttt
    56341 atcttgcttt agccaaacct gaagtaacta agaatctgct gaaatatcgg cataatcaac
    56401 tgccgcaagc aatccacaac gctcagcaac aaggattaaa aggcgcgctt tatcctatgg
    56461 tcaccttcac tggtgtcgaa tgccataacg agtgggaaat cacattcgag gagattcacc
    56521 gaaatggcgc gattgcctat gcaatctaca attatacgaa ttacaccggc gatacggaat
    56581 atttgaaaaa tgaaggactg gaagttttag cagaaatcgc acgcttctgg gcagaccgtg
    56641 tccatttttc aaaacgagca aataaataca tgatccacgg tgtgaccggc ccaaacgaat
    56701 atgagaacaa tatcaataac aattggtata caaatacgat cgcagcttgg gtcttgcgtt
    56761 acacgcgaga aaattattta acttatcaaa aagaagctgc tatcactatc agcgaagaag
    56821 aactagccaa atggaaagat atcgcagaga acatgtactt cccagttgac gaagaattag
    56881 gaatctttgt ccagcatgac acattcttag ataaagatct catgcctgta tccgatattc
    56941 ccacaagcga tcttcctctg aatcagcatt ggtcttggga caaaatcctt agatcttgct
    57001 tcatcaagca ggcagatgta ctacagggga tttatttctt caatgatact tttacaaaag
    57061 aagaaaagca aaaaaacttt gatttctatg aaccaatgac tgtccatgag tcttcccttt
    57121 cgccaagtat ccattcgatt ttagcagcag aattaggaat ggaaagcaaa gctgtagaaa
    57181 tgtaccagcg aacagctcgc ttggatttgg ataattacaa taacgacaca gaagacggct
    57241 tgcacatcac ttccatgact ggtagctggt tgtcgattgt ccaaggattt gctcaaatga
    57301 aaactgccca tgatcagttg agattcgcac catttctgcc aaaaacttgg aaagcttatt
    57361 ctttccatat caattatcgc ggacgtttat tgttcatctc agtcacagat gaacaagtta
    57421 ctttcgagtt attagaaggt tctcctctag aaatttacgt ctatgaacag tcttattctt
    57481 tagcggatcg tctagaaata acgctaagaa aggaggaagc aaatgtttaa aggggtattg
    57541 tttgatttag acggtgtcat taccgatact gctgaattcc attatcgtgc ttggcgaaag
    57601 cttggtcagg aaatcggcat tacgatcgac cacacattca acgaacagct aaaaggcgtc
    57661 agccgagaag actcactctc tttgctttta gcttatggcg gaaaagaaca ctctttttca
    57721 aaggaagaat ttgcagaatt agcgaaaaga aaaaacgatt attatttaga gatgatccag
    57781 acgatcgaac caaaagatgt cttccccggg gtcgtgcctt tactggattc tttaaaagaa
    57841 gaaaaaatca agattgctct tgcttctgca agtaaaaacg ggccattcct acttgaaaaa
    57901 atgggtctta caccttactt tgatgctatt gccgacccgg cagaagcaac aaacggaaaa
    57961 cctgcgccag atatcttcct ccttgcagca aaagctgtcg gactaaaagc agaagaatgt
    58021 atcgggatcg aagacgcgca agccggtatc caagccatcc tctcaagtgg cgctcaacca
    58081 attggcgtgg gacgtgctga agaccttgga gaggagatcc cgcttgtccc tgatacgact
    58141 tttcttacga tcgattactt gaaagaaaag tggcatgacc atggataatc cagagatctc
    58201 cgtcgtacca gttccagaga ctgaaataaa gaaaatcaca ttgaagaacg actacttaga
    58261 agtcgttctt ctcaattacg gtgcccgcct gcaccaaatc ttcgcaccag acaaagaagg
    58321 aaaatcagaa aatgttttat tgtcttacga tcgtttcgaa gatgttctaa cagacaaaag
    58381 cttttttgga gcaactgtcg gacctgtagc cggaaggatc cgggatgctt cttggggtga
    58441 gcatagattg gagaaaaact gcggatcaca ccatatccac ggtggcacga aaggctggtc
    58501 tttccaattt tgggaagtag aagttttcaa aactccatca tcaataggtg tggtctttta
    58561 cttaaaagat gaattttcta cctatccagg accaatcact gctacgatca cttaccgttt
    58621 aacaaaaaac gaattagaga tgacaacctc tgctagtagc cttgttgaaa ccatctgcaa
    58681 tccgaccaac catgcctact tcaacctttc aggaaatgga acaagagaca tctatgaaca
    58741 tcagttaacg gtctttttag atggtgtgct tgaattagat caagaaaaac tgcctactgg
    58801 aaattggaca aaaaaagaaa aactgccaat cgactttaga aaatccccaa cactgcaaga
    58861 gatccttgca tgttatccag atggactaga tgatgtattt cttttgcatc atcctaggct
    58921 ttccaaaact tccttacaat tattcgagaa gcattctggt cgccaaatga ctatagcaac
    58981 ttctaataaa agtatggtat tattttctac gactggattc gaagctgatt tctctgtcaa
    59041 tggaaaacag atgcattcaa actacggatt agcaatcgaa ccgcaagaaa tccctgatat
    59101 cgttcatttc cccaaatttg gatcgatcaa cctccatcct ggtcaagaac ggatcagtca
    59161 gacgatttat cggttctctg cacaataaca agagaaaggt gtttttatca tggctattac
    59221 cgtaaaagat gtggcgaaaa aagccggtgt tgccacctcg accgtctctc gtgtcatcaa
    59281 cgatcatcct agtatttccg agacaacaaa gaagaaagtc cgaaaaatta tggatgaact
    59341 aggttatgtt cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg
    59401 cgtaatctta ccccctcttg attcaaaaga gcggatcggt aacccatttt atcttgagat
    59461 catggaagca atcaacgaag aagcgcggaa ttatgatatg actacagcaa tcgctactgc
    59521 taaaaatttt gaattgctgt tagagaacgt ccagcgtatg catttacaaa aacaagtaga
    59581 tggttttatt ctagtgtatt cagatagtca agatactgtc attgattatt tataccaaaa
    59641 ccacatccct ttcactttga tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt
    59701 agataatgat aaccagcttc taggtaaaca agcaacagaa tttttgatcc agaatggaca
    59761 taagaatatc ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg aacgatattt
    59821 cggttatcaa aaagcaatga tgatggctgg tcttcctgcg ttatccagtg tagtactaga
    59881 gcatccggag gattatctgt cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt
    59941 ggtggtcatt gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa tgtatggcta
    60001 tgtcattcca gagacactct ccgtcattag cttcaacaac tcgatcttct caacattaac
    60061 acacccttac cttacaagta tcgatatcga tatctccgaa ttaggacgtg tcgctatgca
    60121 aaagctgcgc gaattgatcc atgaagaatt gagcagtggc gtacgtttgg tcatccctca
    60181 tcgcttgatc aaaagagaga cagtcgttcc tttagaaaac ccatactaaa tagctgactc
    60241 actaaatacg ctgaccattt atcctatggt cagcttttta tgtaaaagaa gatactactt
    60301 ctaacacatt gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac acttttgtct
    60361 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa
    60421 attcacaaaa atttaaaaag caattttctt gagtttcttc ttatttcttg gagttaaacg
    60481 cttctgtccc agcctctttt ttccttcctt caatttttta cacgtcgacg attcaataaa
    60541 aaaccgccta atattgtaaa gacaggtgca agaatcgaga aaaaggcgaa tggcaagtaa
    60601 tgcaacgcgc taactttcat cgttcccata atgaatgtgc cgctgactcc ccacggtacg
    60661 agagaattga cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt ttttgataag
    60721 ccaacgtcat caaaagaacg cttgaatgtt tctcctggaa gaatgatgga cagatactgt
    60781 tctccaacga tcagattgat tccgatacag ctcaaggcag taaacccgac taaacgagaa
    60841 ggtcggtcca tcttttcttt taattcttga acgatggtat caacgatcag atatttgatc
    60901 agtaacccac ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa catactcatg
    60961 atcccgcctc gagaaagcag ttcatctagc tttggatcag gtgtatttgc gacataacca
    61021 ttcatcaaga tgtctgctgc ttttccgacc gtcaaacttt tctcatggat aaaagaaaga
    61081 acgagagcag ctgtcgatcc cgctagtaat gtagggatag ccggaatttt tttgattgcc
    61141 cccccaaata aaataactag tggaatcaat gtccaaggag agatccaaaa gctcgctttc
    61201 atgttttgga tcatttcatt gatactagct agatcggctg aatgactgtt atgtcctaat
    61261 atcgtataaa aaatcaacga aaggacgaat gctgggaaat ccgtataacg cataccagcg
    61321 atatgttcga atagattaac tcctccaata cccgctgcca agttggtcgt atctgataaa
    61381 ggcgagcagt tattccctaa aaaggcaccg gaaacgactg cgcctgtagt cattgcatca
    61441 ttgaatccaa gtaaatgacc gatccccaaa aaagcaattc caatcgtcga aatcgttgta
    61501 aatgaactgc cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag
    61561 aaacgcacag aaacaagttt aaatccataa accataatcg tagggatggt tccagataaa
    61621 atccatgaag caactaagac accaatcaaa agaaaaatca ggattggaat gatccctgga
    61681 gtgattcctt tgatgatccc gtcatggatc tcatcccatg tgaacttttt aaatctgcca
    61741 taaaacaaca aacacatgaa agctgcaagg atcgggatat gtggcgagag ctggaatccg
    61801 atgatcagta ctcctaggag tgccaacaac acgatcaata cagcgatact ttcttttaaa
    61861 gaaatatttt tattcatcgt cgatctccta tttcaaatga aaaggccctg ccatgaaaaa
    61921 actattcact tggcaggacc tcctactaaa tcagcgacaa aacgtcggct gaaagactta
    61981 tttttttatt tttcttctaa acttttttct acctcaatcc cccaagaatc tttcttgaag
    62041 tgattgatga tcaatggaat aacgaacatg accactgtga tagctgctac taataatgca
    62101 tcactcaatg tcgctgtacc taatgtagca aatacgacaa cgatgataga gaagatcagc
    62161 atgcagctga ctacccaagc taatccgttt ccgtttttac caattcggta aggacgttcg
    62221 aattctggat gtttcttacg cattttaagc aatgaaacag caatcaaaat gtacacgata
    62281 caataaataa cagttgtagc atttgtaagt gtcaagaata cagcacttac gtcatccatt
    62341 acaccataaa gcaaagcaaa taatgaaata acgattgatt gtgttagaac gacatttcta
    62401 gatacgccgt atttgttttc tttatggaat ccgaattttg gtggaaggaa tccttcacga
    62461 gctacttgga tgattgtttt tgatggtcca gtaacccaag ctgataattg taaaagcaca
    62521 ccaataaata ccatgaagct gaagatattt ccgatcactt ctggtaagcc taggatttga
    62581 cagtcaatca agataggctg cgtgatattt gctaattcca tttttccatc tggtacgaca
    62641 tttgctacaa gcatcgcatt gatgacattc aataaaacta atccgatcaa agcgataaat
    62701 acgccttttg tataattttt tgttgcgtct ttcaaacgag gcatgtaaac agaactcatt
    62761 tcgatcccaa caaaaataaa tgtgatacca gctaagtatt tcaatgaatc aatatgttcc
    62821 aagtcaggga aagcttttga ccatgaaaag tcacctagat agccgtttga gactagtccg
    62881 actttgaagg ctgccaaaac acctaaaacg aacatgatga ctactgggat atatacccct
    62941 agccatacac cgatgttccc gccgactttt gccatatcga atttcaagtt caagatcgtg
    63001 atgatccagt agataactaa aatacaacct aaaacgaacc agtgattatt gccaagttct
    63061 acattaccaa atgtgtttcc taacaatggt cctaaagtag aagcgaccat taccatacct
    63121 gggaacattt gcacccataa tagccatgca acgacaaatc cccatttgct gcccagtgct
    63181 gtttttaccc ataactgagg accgccttct tgaggaagca tcgtggaaag ctcaccggaa
    63241 accattgata tcggaccggc aaaaaacaaa actgcaaaaa ttgaataaaa aatcaatgtc
    63301 caaccgactg cggctaacgt aggaatactc cgaaccgttg cacacagtgc cattgtcaga
    63361 ccgataaagg tcccaagacc cattttactt acttcagatt tactcaccgt tgacactctc
    63421 cttgattttt tggataaaat aacggtgtct ttgaagtagt ttcagcaata aacgaaacaa
    63481 ttcagcgaat atttgagggg attctttgag tttattgctt gaagggtttc ttcaaagaca
    63541 acctcaaaac aaagggatcg aagagcttag gtctcccctt gctcttcaga tccaaacatg
    63601 cgtcagaaac agttagtatt caagctgttt tctaacatga ttaccttgct tattttgctt
    63661 cgcttgccaa taattggtct gcgtagattt tttccaattt ttcttgaaga gctgctttga
    63721 ttttttcagc atattcttca aagtgttctt ctttgttcat gtatggtgtc attacgctgg
    63781 cacgcaatac agttactttt ccggcacggt tccattcttc atctgagaat cctagttggt
    63841 tcacaaattg taatgggctg ttgccatagt ctgggatagc aaagtcagta tgtgaagtta
    63901 agaactcatt accgtaaatg cttcctttca cgtatgaaga ataatcatat acgtcatggt
    63961 tcaatttatt cattgcaact aagtcatcgt tgcctttttc tttgaataca tagtctacca
    64021 tattgaaatc tggataagtt aatggatgaa cttcgatttc tttgtctcct actttaaatg
    64081 acaagtcatt caagaagttg tagaaacgat gtgcaccttc gattgatgca cccattaatt
    64141 taccatatcc tgtgacattc aaaggtaaga catggtgtgc tgcccaaacg ctggctgctg
    64201 ttgcccctgc ttttgaacct tccaagatat aagccccaag taatgctggg atatctgcgc
    64261 ctttttcaaa tacatatgtc gcaaagtaag agataacgtc tctcatgcgg atatctttga
    64321 tgacgatacc accggcagaa tatggaacat agcccatttt atgtgggtca atcgttacag
    64381 attctgcttc ttcgattgct ttatatgcac tgtgtacttc ttctaagata taatctttgt
    64441 tttctgtaaa gacattgtat ttgtaatgaa catcttttaa atcttcaaat ggaatgaagt
    64501 tgttgtcttc gtctaagaaa atcgcacggc cgtatccgcc ataagcagca tctacgtgta
    64561 agtagaagta aataccgtct ttttctaata cgcgacgcaa tgcaacgatt ttatcgatac
    64621 cgtcgattgc tccttcttct gttgatccta caacaccgac tacacctagg attggtgttt
    64681 tttcagctgc tagaccacga acgatttttt caagttcgtt gatgtccatg cgatagttgt
    64741 ggtcaactgg cactggaatc acttggtcta aaccgatacc gatgatgtcg gcagctttta
    64801 gccatgaata gtgtttagtt tgtggaacca accattttcc taatttttct aaatgttttc
    64861 cgctacgtgc tgagtgagct ttgatttcgt cgattttttc tgggacgctg tctaacagat
    64921 tcatgatttc ttctgttgac aagttcatta attcccaatc gctttttcca gcaactagtt
    64981 caggtgtcac ttctttcatt gcaagcggca atgatttgat gttgcgggca taccaaagtc
    65041 cttctaagtt tgctaaagaa ccatcagcaa cgatgtggcc ccagccatct ttatagctca
    65101 ttaattttgc aaattccatt cctacttctt cttccatttg gcttgttgca ggagaagatt
    65161 cataggcaac gttattacca ttccatagca tagcaaaatt gtacgctagt aaagatggca
    65221 tcaatgtttc agagttcata tgaccccagt aacgtccggc attatgccaa ggaacggaat
    65281 gggtacgcat ccgcgctgaa atttctgata gaacatcctt tgttttattc acagtgtgtt
    65341 caaaactagc actgcttttt tcttctggcg taataatagg catgtcttga ggcatgtagt
    65401 tttgacgcca gcctaaatgt tcatccacca attcattcaa caaagcttta tagatttggc
    65461 cgttctcagc tttgtcccca atgaataggg catttaggtt tagatctttc gacaatgatt
    65521 cactcatagt gaaaccctcc taaaaaaata tttaaatttt gtcgagccct acattacacc
    65581 tgttcttcta ttctgtaaac taagttttgg cctgattttt aatcatattt taatatgtat
    65641 caattatccg aattgttcac acttttgaaa taattaaaaa aaacgaggga atttacaaat
    65701 gtaaactccc tcgcaaagat tgtattaacg acaaccagcg ttctatttta ggatgggttt
    65761 caaaattgga atgttgtaat agtatttccc tttttcaagg taaaaactta ctaaattcta
    65821 atcaattact ttggccaaaa agtaattttt cttacctttt cgaataacca caaacttacc
    65881 atcgaattca tcggatggat ttatgacaaa atttaaatcg gtaacgcggt caccgttaat
    65941 acttatagct ccgtttgaaa catcttcacg tgcttgacgt ttagatggtt caatttttgt
    66001 ggaaacaagc agttccacga tattttcagg tgtgcttgaa atttcaacgt tcggcatttt
    66061 accgaatcct tgcgcgattt cttccgcatt tagttcttta atatttccag agaacaatgc
    66121 ttgcgttatt ttttgcgctt ctttcaagtc ttcttcacta tgcacaaatc ttgtaacttc
    66181 ttctgccaaa cggcgttgtg cttcacgttt ctcaggttct gtctctactt ttttcgctaa
    66241 gtcttcgatt tcttcttgag acaagaaagt aaagaatttc aagtatttca ctacatcgcg
    66301 atcatcttga ttcaaccaga attggtagaa ctcaaatggt gaagttttct tcggatcaag
    66361 ccaaacggca ccgcctgcag tttttccgaa tttcgttccg tcagccttca gcatcaatgg
    66421 gatcgttaaa ccgaacgctt ctgcttctgg tccttctaat tttcggatta ggtctaatcc
    66481 agctgtgata tttccccatt ggtctgctcc accgatttgc aattgcacat cgtatgtttt
    66541 atgcagatgc aaaaagtcga tagattgcag gatttggtaa gtaaactctg taaatgagat
    66601 tcctacttcc aaacggctgg caacgatatc ttttgctaac atcgtgttga tgttgaagtt
    66661 tttaccgtag tctctcaaaa attcaagtaa agagattttt gacaaccagt cgtagttgtt
    66721 cacaaatgtg atatttgcat ctttaccaaa caattttctc atttgattgg aaagtgcgtc
    66781 cacgttatgt tgcacttgtt ccatcgtttg caacacacgt tctgttttgc ggccgcttgg
    66841 gtcgccgatc gttcctgttc cgccgccaat cagaatatat ggatgatggc ctgccaattg
    66901 gaatcttttc atcatcataa atggaatcaa atgtccgata tgcatcgaat caccagttgg
    66961 atcaacgccg caataaagtg aaatgctctt ttcttcaacg agctcgcgaa gtctttcttc
    67021 gtttgtttgc tgattaatgg cgtcgcgcca agttaattca tcaataatat tcattttact
    67081 gacctccgtg tagttttttt atgaaagcag gaacctgcta gcatatgtaa ttaattattt
    67141 cttcaggata ggataggaaa taaaaaaagt cccttggcat ataagccaag ggacgaaatc
    67201 tcgcggtacc acccaatttg tatgtctaga tatccaacat acctctcgaa catttaacgc
    67261 tgtcgcgtgg caatcactgc caaagactcc tgaaatgtaa ttcgctttaa agcatgtact
    67321 ggtttgcagc gaccaccagc tttctgaaac attggcttta acaactactt cgtttcgatc
    67381 ttcgtcttgt tattattaat atgtgtctat catagagcgc aactttttct ttgtcaacag
    67441 aaaaaggata ttttttttaa attcctcatt ttttcttttt tgaatccgct ctaattccga
    67501 acattttatt ttcaattatt gttaaaagaa ataacttttt ctgtattttt tctgcaattt
    67561 atcgagaagt cactttactt tgcctcttat tctttcttcg ttacaataag gaatgggaca
    67621 agtaaaaaaa tgaaaaagca agggacgagg tatatggaaa aaaagcaaaa aattatttta
    67681 ggagccactt ctgctttaac tatcgttatt ttaattctaa gtttgttttt tgtcatccga
    67741 tcatctacaa aaaaagaacc aatcattcct gaaaaaaaaa taacagaaaa agcaaaagaa
    67801 ccgacagatg ataagcaaca actagttact caagcaaaaa atctggctca aagctacgat
    67861 tatgaccaag cgattgcgtt attggaaaaa gatagtaaga acggatctac tgaaacacaa
    67921 cagcttcttc aaacactaaa aaaggagaaa gaacaactga tcgactggtc tgatcctact
    67981 cagatctctc atgtcttttt ccacagcttg atcgtggacc cgcaaaaagc cttccactct
    68041 caacaggcac aaggatacaa agattatatg gtgactgttg aagaattcaa ccgttcgatc
    68101 gaacaacttt atcaaaatga ttttgtttta gttaatttga atgatttgat ccaaaaagac
    68161 gaacaaggaa atctttcctt taacgggctt gcgctacctg aaggaaaaaa accattggtt
    68221 ctttcacaag atgatgtcag ctattatgaa tatatggata attccggttt tcctagccga
    68281 ttggtcgtat caaataacaa tgaaatcaaa aatgtctata aagagaaaga taaagaagaa
    68341 atcggtgact ttgacatggt tcctctgatc gatgcctttg tcaagaaaca tcctgatttt
    68401 tcttatcgag gcgcaaaagg taccttggcc ctgacaggtt acaacggagt tttaggttat
    68461 cgaacatcaa aatctcaata tggagacaat gaaaaaacaa aaaaagagat taaagaagct
    68521 aaaaaagtgg cggaacagtt gaagaaagac ggctggagct ttgcttccca tacatggggt
    68581 cacttgaata tgactcaagc ttcactagaa gatgtaaaaa aagacaatga attgtggcag
    68641 caagaagttg cacctatcct aggaaaaaca aatattctga tttatccatt tggtgcagac
    68701 atcagcgact ggcagcctta ttcagcagaa aatcaaaaat ttgcttactt gaagagtcaa
    68761 ggtttcgata ttttctgtaa tgtcgatgct tctacaccag cttggggaca attaggtacg
    68821 gatttctatc gaaatgctcg gatcaatatc gatggcattc gttttcaatc tgatttagac
    68881 ggaaaaaatc ctattcttga tccattcatc aatgtccatg atgtctatga caaagaagca
    68941 cgcgaatctg cttgatttta cgtcatttat gaaccaaaaa agccgaactg acattttatc
    69001 gtcagtttcg gcttttttat gctttatttc ttcttcggtg cgattccctt gatcaggact
    69061 tctgtccatt ctgctgtata tggaagcagt tttgcgtata tctccggctt agaaattcct
    69121 tctaccattg cttgaaaata gaggatcaag aactcatccg aatatttcaa atccactttt
    69181 ccttctttcc gccctcgatg gaaaagatca agcatgatac tgaaactttc ttgcgtatac
    69241 gcatccatcg cgtcagaaaa ttcttttttt tctttttttg tatagtatgt catcaaatcg
    69301 atataaaatt gtttgttcac tcgattgaga taacggattt tgttttcact catggcaatc
    69361 agtgtttctt caaatggaag attttccgca atcaacgctt tcgcttcttg ccctaaatca
    69421 ttcataaatg caaaaaatac ttcataaatc agcccttcct tgctgccaaa atatttaaaa
    69481 atcgtcgctt tacttacctg agcgttctca gctaccttat ccattgtcac tccagcggtt
    69541 cctatgtctg tgttcatcaa gtcaaaagct gcttgtaaaa tctctttctt tttctcttgt
    69601 gctctctttt caaaaccgtt catttgtaga gcctcccgta attttaaact tttataatat
    69661 atatagttc
//
LOCUS       DAFCHQ010000006        62293 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000006 DAFCHQ010000000
VERSION     DAFCHQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.006"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            476..1339
                     /locus_tag="LNC71_000436"
     CDS             476..1339
                     /locus_tag="LNC71_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6465534.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="LNC71_000437"
     CDS             complement(1468..3090)
                     /locus_tag="LNC71_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6465535.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="LNC71_000438"
     CDS             3339..3860
                     /locus_tag="LNC71_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK6465536.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LNC71_000439"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LNC71_000439"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBK6465537.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="LNC71_000440"
     CDS             5941..6759
                     /locus_tag="LNC71_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBK6465538.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="LNC71_000441"
     CDS             complement(6862..7059)
                     /locus_tag="LNC71_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465539.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="LNC71_000442"
     CDS             7228..7806
                     /locus_tag="LNC71_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK6465540.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="LNC71_000443"
     CDS             7901..8038
                     /locus_tag="LNC71_000443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465541.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="LNC71_000444"
     CDS             complement(8851..9354)
                     /locus_tag="LNC71_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6465542.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="LNC71_000445"
     CDS             9713..10738
                     /locus_tag="LNC71_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6465543.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="LNC71_000446"
     CDS             11061..11939
                     /locus_tag="LNC71_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBK6465544.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="LNC71_000447"
     CDS             12415..12531
                     /locus_tag="LNC71_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK6465545.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="LNC71_000448"
     CDS             13459..13797
                     /locus_tag="LNC71_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465546.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="LNC71_000449"
     CDS             13891..14121
                     /locus_tag="LNC71_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465547.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="LNC71_000450"
     CDS             14521..14892
                     /locus_tag="LNC71_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBK6465548.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="LNC71_000451"
     CDS             complement(15166..15858)
                     /locus_tag="LNC71_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6465549.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="LNC71_000452"
     CDS             complement(15861..16646)
                     /locus_tag="LNC71_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6465550.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="LNC71_000453"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="LNC71_000453"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBK6465551.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="LNC71_000454"
     CDS             18308..18625
                     /locus_tag="LNC71_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6465552.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="LNC71_000455"
     CDS             18638..20311
                     /locus_tag="LNC71_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBK6465553.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="LNC71_000456"
     CDS             20325..21956
                     /locus_tag="LNC71_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBK6465554.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="LNC71_000457"
     CDS             complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="LNC71_000457"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBK6465555.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24071..24994)
                     /locus_tag="LNC71_000458"
     CDS             complement(24071..24994)
                     /locus_tag="LNC71_000458"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBK6465556.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25190..25795
                     /locus_tag="LNC71_000459"
     CDS             25190..25795
                     /locus_tag="LNC71_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6465557.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26103..27119
                     /locus_tag="LNC71_000460"
     CDS             26103..27119
                     /locus_tag="LNC71_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6465558.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27260..28561
                     /locus_tag="LNC71_000461"
     CDS             27260..28561
                     /locus_tag="LNC71_000461"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBK6465559.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28607..29269
                     /gene="deoC"
                     /locus_tag="LNC71_000462"
     CDS             28607..29269
                     /gene="deoC"
                     /locus_tag="LNC71_000462"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBK6465560.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29290..29688
                     /locus_tag="LNC71_000463"
     CDS             29290..29688
                     /locus_tag="LNC71_000463"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBK6465561.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29754..30836
                     /locus_tag="LNC71_000464"
     CDS             29754..30836
                     /locus_tag="LNC71_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6465562.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31048..32610
                     /locus_tag="LNC71_000465"
     CDS             31048..32610
                     /locus_tag="LNC71_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465563.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32603..33736
                     /locus_tag="LNC71_000466"
     CDS             32603..33736
                     /locus_tag="LNC71_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465564.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33736..34692
                     /locus_tag="LNC71_000467"
     CDS             33736..34692
                     /locus_tag="LNC71_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465565.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34791..35957
                     /gene="deoB"
                     /locus_tag="LNC71_000468"
     CDS             34791..35957
                     /gene="deoB"
                     /locus_tag="LNC71_000468"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBK6465566.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35981..36799
                     /locus_tag="LNC71_000469"
     CDS             35981..36799
                     /locus_tag="LNC71_000469"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK6465567.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36816..37523
                     /gene="deoD"
                     /locus_tag="LNC71_000470"
     CDS             36816..37523
                     /gene="deoD"
                     /locus_tag="LNC71_000470"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBK6465568.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="LNC71_000471"
     CDS             complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="LNC71_000471"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK6465569.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38679..39260)
                     /locus_tag="LNC71_000472"
     CDS             complement(38679..39260)
                     /locus_tag="LNC71_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6465570.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="LNC71_000473"
     CDS             complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="LNC71_000473"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBK6465571.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40395..40808
                     /gene="rpsL"
                     /locus_tag="LNC71_000474"
     CDS             40395..40808
                     /gene="rpsL"
                     /locus_tag="LNC71_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBK6465572.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40892..41362
                     /gene="rpsG"
                     /locus_tag="LNC71_000475"
     CDS             40892..41362
                     /gene="rpsG"
                     /locus_tag="LNC71_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBK6465573.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41456..43543
                     /gene="fusA"
                     /locus_tag="LNC71_000476"
     CDS             41456..43543
                     /gene="fusA"
                     /locus_tag="LNC71_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBK6465574.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43715..44902
                     /gene="tuf"
                     /locus_tag="LNC71_000477"
     CDS             43715..44902
                     /gene="tuf"
                     /locus_tag="LNC71_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBK6465575.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45309..45617
                     /gene="rpsJ"
                     /locus_tag="LNC71_000478"
     CDS             45309..45617
                     /gene="rpsJ"
                     /locus_tag="LNC71_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBK6465576.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45648..46277
                     /gene="rplC"
                     /locus_tag="LNC71_000479"
     CDS             45648..46277
                     /gene="rplC"
                     /locus_tag="LNC71_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBK6465577.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46305..46928
                     /gene="rplD"
                     /locus_tag="LNC71_000480"
     CDS             46305..46928
                     /gene="rplD"
                     /locus_tag="LNC71_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBK6465578.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46928..47218
                     /gene="rplW"
                     /locus_tag="LNC71_000481"
     CDS             46928..47218
                     /gene="rplW"
                     /locus_tag="LNC71_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBK6465579.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47257..48090
                     /gene="rplB"
                     /locus_tag="LNC71_000482"
     CDS             47257..48090
                     /gene="rplB"
                     /locus_tag="LNC71_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBK6465580.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            48133..48411
                     /gene="rpsS"
                     /locus_tag="LNC71_000483"
     CDS             48133..48411
                     /gene="rpsS"
                     /locus_tag="LNC71_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBK6465581.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48434..48781
                     /gene="rplV"
                     /locus_tag="LNC71_000484"
     CDS             48434..48781
                     /gene="rplV"
                     /locus_tag="LNC71_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBK6465582.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48795..49451
                     /gene="rpsC"
                     /locus_tag="LNC71_000485"
     CDS             48795..49451
                     /gene="rpsC"
                     /locus_tag="LNC71_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBK6465583.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49454..49888
                     /gene="rplP"
                     /locus_tag="LNC71_000486"
     CDS             49454..49888
                     /gene="rplP"
                     /locus_tag="LNC71_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBK6465584.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49878..50066
                     /gene="rpmC"
                     /locus_tag="LNC71_000487"
     CDS             49878..50066
                     /gene="rpmC"
                     /locus_tag="LNC71_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBK6465585.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            50091..50357
                     /gene="rpsQ"
                     /locus_tag="LNC71_000488"
     CDS             50091..50357
                     /gene="rpsQ"
                     /locus_tag="LNC71_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBK6465586.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50415..50783
                     /gene="rplN"
                     /locus_tag="LNC71_000489"
     CDS             50415..50783
                     /gene="rplN"
                     /locus_tag="LNC71_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBK6465587.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50820..51128
                     /gene="rplX"
                     /locus_tag="LNC71_000490"
     CDS             50820..51128
                     /gene="rplX"
                     /locus_tag="LNC71_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBK6465588.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            51156..51695
                     /gene="rplE"
                     /locus_tag="LNC71_000491"
     CDS             51156..51695
                     /gene="rplE"
                     /locus_tag="LNC71_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBK6465589.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51714..51899
                     /locus_tag="LNC71_000492"
     CDS             51714..51899
                     /locus_tag="LNC71_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBK6465590.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51935..52333
                     /gene="rpsH"
                     /locus_tag="LNC71_000493"
     CDS             51935..52333
                     /gene="rpsH"
                     /locus_tag="LNC71_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBK6465591.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52365..52901
                     /gene="rplF"
                     /locus_tag="LNC71_000494"
     CDS             52365..52901
                     /gene="rplF"
                     /locus_tag="LNC71_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBK6465592.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53062..53418
                     /gene="rplR"
                     /locus_tag="LNC71_000495"
     CDS             53062..53418
                     /gene="rplR"
                     /locus_tag="LNC71_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBK6465593.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53439..53939
                     /gene="rpsE"
                     /locus_tag="LNC71_000496"
     CDS             53439..53939
                     /gene="rpsE"
                     /locus_tag="LNC71_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBK6465594.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53954..54133
                     /gene="rpmD"
                     /locus_tag="LNC71_000497"
     CDS             53954..54133
                     /gene="rpmD"
                     /locus_tag="LNC71_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBK6465595.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            54177..54617
                     /gene="rplO"
                     /locus_tag="LNC71_000498"
     CDS             54177..54617
                     /gene="rplO"
                     /locus_tag="LNC71_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBK6465596.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54617..55912
                     /gene="secY"
                     /locus_tag="LNC71_000499"
     CDS             54617..55912
                     /gene="secY"
                     /locus_tag="LNC71_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBK6465597.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55974..56621
                     /locus_tag="LNC71_000500"
     CDS             55974..56621
                     /locus_tag="LNC71_000500"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBK6465598.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56812..57030
                     /gene="infA"
                     /locus_tag="LNC71_000501"
     CDS             56812..57030
                     /gene="infA"
                     /locus_tag="LNC71_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBK6465599.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            57063..57179
                     /gene="rpmJ"
                     /locus_tag="LNC71_000502"
     CDS             57063..57179
                     /gene="rpmJ"
                     /locus_tag="LNC71_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBK6465600.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            57197..57562
                     /gene="rpsM"
                     /locus_tag="LNC71_000503"
     CDS             57197..57562
                     /gene="rpsM"
                     /locus_tag="LNC71_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBK6465601.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57590..57979
                     /gene="rpsK"
                     /locus_tag="LNC71_000504"
     CDS             57590..57979
                     /gene="rpsK"
                     /locus_tag="LNC71_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBK6465602.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58059..58997
                     /locus_tag="LNC71_000505"
     CDS             58059..58997
                     /locus_tag="LNC71_000505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBK6465603.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59025..59405
                     /gene="rplQ"
                     /locus_tag="LNC71_000506"
     CDS             59025..59405
                     /gene="rplQ"
                     /locus_tag="LNC71_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBK6465604.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59677..60516
                     /locus_tag="LNC71_000507"
     CDS             59677..60516
                     /locus_tag="LNC71_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6465605.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60492..61361
                     /locus_tag="LNC71_000508"
     CDS             60492..61361
                     /locus_tag="LNC71_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6465606.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61351..62148
                     /locus_tag="LNC71_000509"
     CDS             61351..62148
                     /locus_tag="LNC71_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK6465607.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        19716 a        11158 c        13248 g        18171 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgggatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg
    22501 gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg
    22561 gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt
    22621 gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt
    22681 gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc
    22741 atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc
    22801 aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt
    22861 ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg
    22921 catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt
    22981 accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc
    23041 accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt
    23101 acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt
    23161 gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc
    23221 gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt
    23281 ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt
    23341 tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt
    23401 ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta
    23461 ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg
    23521 gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt
    23581 agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa
    23641 gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg
    23701 ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac
    23761 aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact
    23821 gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt
    23881 aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa
    23941 ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc
    24001 aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta
    24061 ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat
    24121 ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat
    24181 tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa
    24241 aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat
    24301 ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa
    24361 agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga
    24421 tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat
    24481 gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga
    24541 tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt
    24601 ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt
    24661 tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta
    24721 cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac
    24781 ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag
    24841 gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat
    24901 ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc
    24961 ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa
    25021 tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct
    25081 ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata
    25141 cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca
    25201 ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa
    25261 aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta
    25321 tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat
    25381 cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg
    25441 gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa
    25501 gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga
    25561 agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca
    25621 tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat
    25681 ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc
    25741 agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc
    25801 tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag
    25861 ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata
    25921 gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa
    25981 ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc
    26041 tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata
    26101 ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct
    26161 cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt
    26221 cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac
    26281 attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga
    26341 tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc
    26401 gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg
    26461 gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt
    26521 atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc
    26581 ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc
    26641 atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca
    26701 acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac
    26761 atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag
    26821 atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg
    26881 atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc
    26941 tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt gatattggat
    27001 tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca
    27061 aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac
    27121 tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt
    27181 agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga
    27241 agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa
    27301 cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc
    27361 agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga
    27421 aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct
    27481 tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat
    27541 tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct
    27601 aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct
    27661 ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc
    27721 aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt
    27781 tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga
    27841 tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc
    27901 aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc
    27961 ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt
    28021 cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc
    28081 cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac
    28141 gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca
    28201 aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta
    28261 cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt
    28321 aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca
    28381 cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact
    28441 attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa
    28501 tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata
    28561 aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg
    28621 aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat
    28681 cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct
    28741 agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct
    28801 aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc
    28861 agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt
    28921 ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat
    28981 tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc
    29041 tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga
    29101 ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg
    29161 tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac
    29221 aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag
    29281 gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat
    29341 aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc
    29401 aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag
    29461 cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta
    29521 gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa
    29581 ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg
    29641 accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt
    29701 cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa
    29761 aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat
    29821 gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg
    29881 cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg
    29941 aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat
    30001 atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg
    30061 gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa
    30121 caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg
    30181 ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt
    30241 atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc
    30301 gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa
    30361 tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg
    30421 ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta
    30481 acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta
    30541 tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa
    30601 acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa
    30661 aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg
    30721 gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa
    30781 aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc
    30841 atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat
    30901 tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa
    30961 tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga
    31021 tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag
    31081 aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag
    31141 gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac
    31201 gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta
    31261 aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt
    31321 atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc
    31381 gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat
    31441 ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg
    31501 gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca
    31561 gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa
    31621 gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac
    31681 cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc
    31741 tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa
    31801 gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt
    31861 ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc
    31921 gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag
    31981 gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa
    32041 atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta
    32101 gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa
    32161 aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat
    32221 gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa
    32281 aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca
    32341 actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg
    32401 gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg
    32461 gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca
    32521 tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag
    32581 ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt
    32641 gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg
    32701 ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg
    32761 gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt
    32821 agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat
    32881 cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt
    32941 atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg
    33001 agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata
    33061 cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc
    33121 ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg
    33181 cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct
    33241 gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga atccttttgc
    33301 ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg
    33361 cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt
    33421 tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg
    33481 atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact
    33541 tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat
    33601 tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg
    33661 gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga
    33721 agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg
    33781 gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg
    33841 atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc
    33901 aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt
    33961 ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct
    34021 gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg
    34081 atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt
    34141 tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc
    34201 ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg
    34261 aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt
    34321 atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg
    34381 ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga
    34441 caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg
    34501 ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca
    34561 gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt
    34621 gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc
    34681 aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc
    34741 gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac
    34801 gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt
    34861 tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc
    34921 cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga
    34981 aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga
    35041 caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg
    35101 gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta
    35161 acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag
    35221 gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa
    35281 tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg
    35341 atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca
    35401 cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt
    35461 ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg
    35521 gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc
    35581 tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg
    35641 cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact
    35701 tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg
    35761 atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat
    35821 tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata
    35881 tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct
    35941 tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat
    36001 gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt
    36061 gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc
    36121 ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt
    36181 ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga
    36241 aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca
    36301 ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt
    36361 gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga
    36421 agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc
    36481 aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg
    36541 ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc
    36601 cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa
    36661 agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca
    36721 tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga
    36781 aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag
    36841 aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg
    36901 cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta
    36961 ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag
    37021 ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag
    37081 gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg
    37141 cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca
    37201 gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc
    37261 atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc
    37321 aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag
    37381 gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg
    37441 gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat
    37501 tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa
    37561 gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa
    37621 tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca
    37681 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
    37741 gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat cgtgtcacag
    37801 tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa gtcatcattt
    37861 aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc ttcgtcagag
    37921 ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc tactaaaaca
    37981 gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa agcgcgttct
    38041 aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat tgcgtaacga
    38101 cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag agtatcgtaa
    38161 gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc
    38221 aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc
    38281 caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg agttaaaaca
    38341 gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc gccttctttt
    38401 gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa ttggttcaat
    38461 gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt tagctctcct
    38521 tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca attttttgga
    38581 agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga gaatataaaa
    38641 aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa ttgctcgatt
    38701 tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc gcattttgaa
    38761 gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt tttgatcgtt
    38821 tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc atctaatcca
    38881 actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc cattaattgg
    38941 aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat caagtgcagc
    39001 ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt gcctaatcga
    39061 tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa
    39121 tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat ttcttgacaa
    39181 ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt
    39241 gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa ctggatataa
    39301 cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt
    39361 ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc gtcaagaatc
    39421 tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa aagcccatgt
    39481 tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg ttcgatgact
    39541 tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc tccgttttcg
    39601 tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag
    39661 ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac taatttagaa
    39721 gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc gaaaagcaaa
    39781 gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc accgtctatt
    39841 gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa tttttccgct
    39901 tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc ttcacgcata
    39961 cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag acctacgatc
    40021 atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa attcatcttt
    40081 gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc gtgatttacg
    40141 gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa ttgcatctct
    40201 tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta caggtttctg
    40261 tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga cattgcattt
    40321 attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa
    40381 ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt aaatcaaagg
    40441 tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag aaaactcaaa
    40501 ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca atgacaccta
    40561 aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag
    40621 ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt gtattattac
    40681 gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc ggtgctttag
    40741 atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact aaaaaaccta
    40801 aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga agtaattact
    40861 atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg
    40921 tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga tcaaccgtgt
    40981 aatggttgac ggaaaacgcg ggattgcagc taacattatc tataatgcct ttgacttgat
    41041 caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga aaaacgttca
    41101 acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag taccagttga
    41161 agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact acgctcgctt
    41221 gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa
    41281 cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg ccaaccgtgc
    41341 gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata agtcgacctc
    41401 agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa ggagaatgac
    41461 cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca tcgatgctgg
    41521 taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata aaattggtga
    41581 aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac gtggtattac
    41641 gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca tcatcgacac
    41701 accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc
    41761 tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag tttggcgtca
    41821 agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc
    41881 tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg ctcacccaat
    41941 ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg taacaatgaa
    42001 ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa ttcctgaaga
    42061 ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag ctgaaactga
    42121 tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg agttaaaaga
    42181 aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg gttcagcatt
    42241 taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc catcaccgtt
    42301 agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag aacgtcctgc
    42361 agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc cattcgtagg
    42421 tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat acgtacaaaa
    42481 cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg ccaactctcg
    42541 ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt tgaaagacac
    42601 aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat ccatggaatt
    42661 cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc aagataaaat
    42721 gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg aaacaaacgt
    42781 ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata tcatcgttga
    42841 ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag tatcttaccg
    42901 tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa
    42961 aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg ctggtttcga
    43021 atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag ctgttgaaac
    43081 tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag tagatatcaa
    43141 agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg ccttccgtgt
    43201 tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa tccttgaacc
    43261 aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta tgggtcacgt
    43321 aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt
    43381 tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc gttctgctac
    43441 tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac caaaatcagt
    43501 acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttcttg aaaccggata
    43561 gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt gtaaaataat
    43621 tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc agacaaataa
    43681 aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat ttgaccgttc
    43741 taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa ctacattaac
    43801 agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg cttacgatca
    43861 aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag ctcacgttga
    43921 gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg cggattacgt
    43981 taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag tttctgctgc
    44041 tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc
    44101 ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat tactagaatt
    44161 agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg acgatgttcc
    44221 tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg aagaaaaaat
    44281 tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg acaacgacaa
    44341 accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta ctgttgctac
    44401 aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc
    44461 tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat tgttagacta
    44521 cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg aagacatcca
    44581 acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat tctctgcaga
    44641 agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca ctaactaccg
    44701 tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac cagaaggaac
    44761 tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc acccaatcgc
    44821 tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt
    44881 cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca tttattcgga
    44941 cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta tttattgcag
    45001 gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa agagtcagta
    45061 ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt
    45121 gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc acagacgtaa
    45181 tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac gctttgccac
    45241 gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata ggcgaaggag
    45301 ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa caccgtattt
    45361 tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg
    45421 gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg actcataaat
    45481 acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc gacattgtga
    45541 acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct ggtgtaaaca
    45601 tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg accaaaggaa
    45661 tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa ttaattccag
    45721 taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg gaaacggatg
    45781 gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca aacaaacctg
    45841 cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa
    45901 atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt ttccaagcag
    45961 gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg gttatcaaac
    46021 gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt cgtcctgggt
    46081 caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg
    46141 gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg
    46201 ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact atcaaatcag
    46261 ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg aatgtagcat
    46321 tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa atcttcggaa
    46381 ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg tcattaagac
    46441 aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc
    46501 gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa tggcgtggag
    46561 gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct aaaaaagttc
    46621 gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa
    46681 tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt cttgctaact
    46741 tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt gctgctttat
    46801 cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt gtgctagacg
    46861 ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt gaggaggtgc
    46921 ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa aatcaatgct
    46981 tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca aaactttagt
    47041 aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt
    47101 acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc
    47161 gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg ctgaataagc
    47221 actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct
    47281 acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac aacatcaaca
    47341 cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa caacggccgc
    47401 atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat cgacttcaaa
    47461 cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc aaaccgttca
    47521 gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt agcacctaaa
    47581 ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa agtcggaaat
    47641 gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga aatgaaacct
    47701 ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa
    47761 ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt agctgcatgc
    47821 cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat cggtaaagct
    47881 ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat gaaccctaat
    47941 gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc accagtttct
    48001 ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa atcagacaaa
    48061 cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc acattttgag
    48121 aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga gcatttaatg
    48181 aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc
    48241 cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga tggacgtaaa
    48301 catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga atttgcacct
    48361 acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta atttgagggg
    48421 aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa aaacagttcg
    48481 cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga
    48541 tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg aaaaagtatt
    48601 gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa acttggtagt
    48661 aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg
    48721 atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat cagaaaaata
    48781 aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta ggcatcatcc
    48841 gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta cacgaagatt
    48901 taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca acagttgaaa
    48961 tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca ggtatggtta
    49021 ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa ttaactggta
    49081 aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca aaattagtag
    49141 gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag
    49201 ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta tctggtcgtc
    49261 taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt cctcttcata
    49321 ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac ggaaaactag
    49381 gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac actgagaaag
    49441 gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa
    49501 aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc
    49561 tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg caatgactcg
    49621 ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat cttatactgc
    49681 caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat gggttgcacc
    49741 agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga
    49801 agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa aacgtgagga
    49861 aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact gccgaaatgc
    49921 ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa ttagcaacag
    49981 gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa
    50041 ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt atgactgaag
    50101 gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa
    50161 ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc atgaactatt
    50221 ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac atcgtgagaa
    50281 ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa gtagtcgaag
    50341 aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca aaatctgaaa
    50401 ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg
    50461 cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact gctaacatcg
    50521 gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc aaaaaaggtg
    50581 acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat
    50641 atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc cctcgtggaa
    50701 cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt
    50761 cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc gaaacagtta
    50821 tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa aacaaagaag
    50881 gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg
    50941 ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt gagatggaag
    51001 cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct ggccgagttg
    51061 gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag
    51121 ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc
    51181 ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt atgcaaacac
    51241 ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca aacgctaaaa
    51301 acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca atgattacaa
    51361 aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag
    51421 ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct gtttcactac
    51481 cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata
    51541 ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta gtagataaag
    51601 tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt
    51661 tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac tacgtggcta
    51721 aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc
    51781 gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt tgccgtattt
    51841 gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa
    51901 aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc caattgcaga
    51961 ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag aagtacctgc
    52021 atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt
    52081 tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa
    52141 cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt
    52201 caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca tttcgacttc
    52261 tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc
    52321 ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa
    52381 ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac aacgttacag
    52441 ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa atgaatatcg
    52501 aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa acaattcatg
    52561 gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag
    52621 cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa cttgtattga
    52681 acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact gttgaagtac
    52741 cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc gaattagctg
    52801 caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt cgttatgttg
    52861 gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat
    52921 agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa aatacatttt
    52981 tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata gtagtacaat
    53041 aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac acgtcaaaag
    53101 agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg cttgaacgtt
    53161 tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg tgtaacgcta
    53221 gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga agcagcacaa
    53281 gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt
    53341 gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa
    53401 aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc gatccaaaac
    53461 atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg
    53521 gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga cacgtaggat
    53581 ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt gaagatgcga
    53641 agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa gtaatcggtg
    53701 cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg gttgctgctg
    53761 gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact tctaaatctt
    53821 taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg aaacaattga
    53881 aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta atcggataag
    53941 gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta tcggacgtcc
    54001 tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca gttcagtggt
    54061 taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc tagtggacgt
    54121 tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc aaacctatga
    54181 aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg
    54241 gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt
    54301 caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc cgtcgtttac
    54361 caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat cttgatgtat
    54421 tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg
    54481 tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac aagaaattaa
    54541 ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg gctggtggat
    54601 caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa aagacattag
    54661 atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg cacatattac
    54721 tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat ttttcaacat
    54781 gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa tgggggtttc
    54841 accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg tgcctcgatt
    54901 tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata
    54961 tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat tcaatcaata
    55021 cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga tcggtttgat
    55081 tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag aaaaaggtat
    55141 cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc ctgaaagtat
    55201 cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat ggaaatctgc
    55261 tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg taacattttt
    55321 ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac
    55381 aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta tctttgctag
    55441 ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat tcggttctga
    55501 aaactggtac aaagtgatga tggagatctt taactataat acagcacctg gtgctacgat
    55561 ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag taaaccctga
    55621 gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac gccctggtaa
    55681 ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg gtgcagtctt
    55741 ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc ttccacaatc
    55801 aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag aaactgcgaa
    55861 acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt
    55921 gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa acaatgaacc
    55981 tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa atcattgatg
    56041 cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg caaaacgaaa
    56101 cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt cctgatgaag
    56161 taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa ggcttcttat
    56221 tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg ctaaaagatt
    56281 tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac
    56341 gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa attttcaacc
    56401 ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat caaagagaag
    56461 atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa
    56521 tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct cgtgaaatcg
    56581 atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc ccgtagccga
    56641 cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata tgtcagtccg
    56701 acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga actgcagttt
    56761 ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa
    56821 gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa
    56881 gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat tcgtatgcat
    56941 tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata cgatcttaac
    57001 cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca ggaggtatta
    57061 tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta
    57121 aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt caaggataat
    57181 tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg ataaacgtgt
    57241 agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa agatcttagc
    57301 tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc
    57361 tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt
    57421 gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc gccgtggatt
    57481 gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc
    57541 tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca tggcagcaaa
    57601 aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg cacatatcca
    57661 ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg cattagcgtg
    57721 gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca
    57781 aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga ctgttgaagt
    57841 aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac aagctacagg
    57901 tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg cccgccctcc
    57961 aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta acgtcattga
    58021 acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc gaaaaaccaa
    58081 gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa
    58141 gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct tctttgccag
    58201 gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct accatcaaag
    58261 gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg ctgaaaatgt
    58321 atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca gtaactgctg
    58381 gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac atctgtagtg
    58441 tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt ggttatgttc
    58501 aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc gattcaatct
    58561 acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga caccgtgaag
    58621 attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg ttggaagcaa
    58681 tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac ttaactgatg
    58741 aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg
    58801 aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg aaacgtgcag
    58861 gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc aaagtccgta
    58921 acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta ggtttaggtt
    58981 tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt tatcgtaaac
    59041 taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact gatttattaa
    59101 tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca actgcagaaa
    59161 aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg
    59221 tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt
    59281 tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc ggatacacac
    59341 gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc ttagaacttg
    59401 tctaaaatca cactaataac atcactttat agatgattct tttagagtgt tatgatgttg
    59461 gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg cttggggagt
    59521 aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac agatatgttt
    59581 gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt tttttgatag
    59641 tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat tgaattagaa
    59701 aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga tgtctccttt
    59761 acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg aaaatcgaca
    59821 cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa agtgggaaat
    59881 caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat ggtttttcaa
    59941 aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt tggtcttgaa
    60001 aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt
    60061 agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca aaaacaacgt
    60121 gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga tgaagcaacc
    60181 agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa
    60241 gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc taatgccaat
    60301 cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa agaaattttt
    60361 tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga aaaactgaag
    60421 agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga agaaaggatg
    60481 gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag ccgaatacac
    60541 catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac agttatactg
    60601 cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg aatgcgcttg
    60661 ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg gatacggata
    60721 ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac
    60781 agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca
    60841 gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg ctggatgaaa
    60901 gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga gtagcaatcg
    60961 ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg gcaggacttg
    61021 atccccaggg cagaaaagaa atgatggaga tgttttggcg tcttcataag gaacagcaga
    61081 tcactattgt attagttacg catttaatgg atgatgtagc caattttgca gattatgtct
    61141 atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc tttcaaaaca
    61201 ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt gcagaagaat
    61261 taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa ttagcagacg
    61321 ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt tggtcgttat
    61381 atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt agccagtttt
    61441 tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt gttggcattt
    61501 ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat ccgaggcatt
    61561 cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt tacaagtggc
    61621 ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt gatgaacggc
    61681 ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact cacgcttact
    61741 acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta
    61801 cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca
    61861 ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt
    61921 gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc tttatttgtc
    61981 agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg ctacaaaggt
    62041 ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac gatggttatc
    62101 attgcttttg tattattaac tgtgctactg atctttttaa gaggataaac gttcagtggg
    62161 ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcaagc agtgtatgac
    62221 aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt atctgaaaag
    62281 aaagaacgaa aaa
//
LOCUS       DAFCHQ010000007        61089 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000007 DAFCHQ010000000
VERSION     DAFCHQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.007"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..1423)
                     /gene="sufB"
                     /locus_tag="LNC71_000510"
     CDS             complement(32..1423)
                     /gene="sufB"
                     /locus_tag="LNC71_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBK6465608.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(1428..1898)
                     /locus_tag="LNC71_000511"
     CDS             complement(1428..1898)
                     /locus_tag="LNC71_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBK6465609.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(1885..3120)
                     /locus_tag="LNC71_000512"
     CDS             complement(1885..3120)
                     /locus_tag="LNC71_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6465610.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="LNC71_000513"
     CDS             complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="LNC71_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBK6465611.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="LNC71_000514"
     CDS             complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="LNC71_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBK6465612.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(5386..6234)
                     /locus_tag="LNC71_000515"
     CDS             complement(5386..6234)
                     /locus_tag="LNC71_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK6465613.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(6258..6944)
                     /locus_tag="LNC71_000516"
     CDS             complement(6258..6944)
                     /locus_tag="LNC71_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465614.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(6937..7971)
                     /locus_tag="LNC71_000517"
     CDS             complement(6937..7971)
                     /locus_tag="LNC71_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6465615.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(8034..8290)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8366..8704)
                     /locus_tag="LNC71_000518"
     CDS             complement(8366..8704)
                     /locus_tag="LNC71_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBK6465616.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(8706..9062)
                     /locus_tag="LNC71_000519"
     CDS             complement(8706..9062)
                     /locus_tag="LNC71_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBK6465617.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(9068..10255)
                     /locus_tag="LNC71_000520"
     CDS             complement(9068..10255)
                     /locus_tag="LNC71_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6465618.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(10446..11291)
                     /locus_tag="LNC71_000521"
     CDS             complement(10446..11291)
                     /locus_tag="LNC71_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6465619.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11525..12490
                     /locus_tag="LNC71_000522"
     CDS             11525..12490
                     /locus_tag="LNC71_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBK6465620.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            12867..13910
                     /locus_tag="LNC71_000523"
     CDS             12867..13910
                     /locus_tag="LNC71_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBK6465621.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(13972..14436)
                     /locus_tag="LNC71_000524"
     CDS             complement(13972..14436)
                     /locus_tag="LNC71_000524"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBK6465622.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            14682..15629
                     /locus_tag="LNC71_000525"
     CDS             14682..15629
                     /locus_tag="LNC71_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6465623.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(15840..17912)
                     /locus_tag="LNC71_000526"
     CDS             complement(15840..17912)
                     /locus_tag="LNC71_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6465624.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17845..18222)
                     /locus_tag="LNC71_000527"
     CDS             complement(17845..18222)
                     /locus_tag="LNC71_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBK6465625.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(18351..18929)
                     /locus_tag="LNC71_000528"
     CDS             complement(18351..18929)
                     /locus_tag="LNC71_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6465626.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            19061..20353
                     /locus_tag="LNC71_000529"
     CDS             19061..20353
                     /locus_tag="LNC71_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6465627.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(20413..21531)
                     /locus_tag="LNC71_000530"
     CDS             complement(20413..21531)
                     /locus_tag="LNC71_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBK6465628.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="LNC71_000531"
     CDS             complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="LNC71_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBK6465629.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="LNC71_000532"
     CDS             complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="LNC71_000532"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBK6465630.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            24737..25336
                     /locus_tag="LNC71_000533"
     CDS             24737..25336
                     /locus_tag="LNC71_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBK6465631.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="LNC71_000534"
     CDS             complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="LNC71_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBK6465632.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(27031..27375)
                     /locus_tag="LNC71_000535"
     CDS             complement(27031..27375)
                     /locus_tag="LNC71_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBK6465633.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(27512..28657)
                     /locus_tag="LNC71_000536"
     CDS             complement(27512..28657)
                     /locus_tag="LNC71_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6465634.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(28837..29811)
                     /locus_tag="LNC71_000537"
     CDS             complement(28837..29811)
                     /locus_tag="LNC71_000537"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBK6465635.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(29891..30766)
                     /locus_tag="LNC71_000538"
     CDS             complement(29891..30766)
                     /locus_tag="LNC71_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBK6465636.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            30896..32803
                     /locus_tag="LNC71_000539"
     CDS             30896..32803
                     /locus_tag="LNC71_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBK6465637.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(33344..36622)
                     /locus_tag="LNC71_000540"
     CDS             complement(33344..36622)
                     /locus_tag="LNC71_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBK6465638.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(36893..37438)
                     /locus_tag="LNC71_000541"
     CDS             complement(36893..37438)
                     /locus_tag="LNC71_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6465639.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(37450..38142)
                     /locus_tag="LNC71_000542"
     CDS             complement(37450..38142)
                     /locus_tag="LNC71_000542"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBK6465640.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(38161..38445)
                     /locus_tag="LNC71_000543"
     CDS             complement(38161..38445)
                     /locus_tag="LNC71_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465641.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(38458..39021)
                     /locus_tag="LNC71_000544"
     CDS             complement(38458..39021)
                     /locus_tag="LNC71_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6465642.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            39249..39911
                     /locus_tag="LNC71_000545"
     CDS             39249..39911
                     /locus_tag="LNC71_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBK6465643.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            40035..41231
                     /locus_tag="LNC71_000546"
     CDS             40035..41231
                     /locus_tag="LNC71_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBK6465644.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(41332..42708)
                     /locus_tag="LNC71_000547"
     CDS             complement(41332..42708)
                     /locus_tag="LNC71_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6465645.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(42745..43611)
                     /locus_tag="LNC71_000548"
     CDS             complement(42745..43611)
                     /locus_tag="LNC71_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBK6465646.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(43700..44725)
                     /locus_tag="LNC71_000549"
     CDS             complement(43700..44725)
                     /locus_tag="LNC71_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6465647.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(44743..45417)
                     /locus_tag="LNC71_000550"
     CDS             complement(44743..45417)
                     /locus_tag="LNC71_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6465648.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(45601..46494)
                     /locus_tag="LNC71_000551"
     CDS             complement(45601..46494)
                     /locus_tag="LNC71_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6465649.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(46568..48265)
                     /locus_tag="LNC71_000552"
     CDS             complement(46568..48265)
                     /locus_tag="LNC71_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6465650.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(48258..49571)
                     /locus_tag="LNC71_000553"
     CDS             complement(48258..49571)
                     /locus_tag="LNC71_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK6465651.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(49571..53608)
                     /locus_tag="LNC71_000554"
     CDS             complement(49571..53608)
                     /locus_tag="LNC71_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK6465652.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(53621..54079)
                     /locus_tag="LNC71_000555"
     CDS             complement(53621..54079)
                     /locus_tag="LNC71_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6465653.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(54377..54895)
                     /locus_tag="LNC71_000556"
     CDS             complement(54377..54895)
                     /locus_tag="LNC71_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK6465654.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(54897..55513)
                     /locus_tag="LNC71_000557"
                     /pseudo
     CDS             complement(54897..55513)
                     /locus_tag="LNC71_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(55510..56373)
                     /locus_tag="LNC71_000558"
     CDS             complement(55510..56373)
                     /locus_tag="LNC71_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6465655.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(56360..57271)
                     /locus_tag="LNC71_000559"
     CDS             complement(56360..57271)
                     /locus_tag="LNC71_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6465656.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(57312..58646)
                     /locus_tag="LNC71_000560"
     CDS             complement(57312..58646)
                     /locus_tag="LNC71_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6465657.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            58854..59684
                     /locus_tag="LNC71_000561"
     CDS             58854..59684
                     /locus_tag="LNC71_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBK6465658.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(59928..60246)
                     /locus_tag="LNC71_000562"
                     /pseudo
     CDS             complement(59928..60246)
                     /locus_tag="LNC71_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(60368..60562)
                     /locus_tag="LNC71_000563"
     CDS             complement(60368..60562)
                     /locus_tag="LNC71_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465659.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        17240 a        13151 c        11000 g        19698 t
ORIGIN      
        1 tatatcaagg gttctagctc ctataacagg tttacccaac actgccttcc atctcataac
       61 tgatcaatcg attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa
      121 caaagcccat gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat
      181 agtagagttg ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc
      241 tgttgtggat ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg
      301 tgtcacattc gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc
      361 gatagtttac ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat
      421 ttggtgcatt gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg
      481 cgatggagag catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca
      541 tcgttgtttt tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt
      601 ttgctcgttt ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac
      661 ggcaatacgc atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg
      721 aataagtagg tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga
      781 tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg
      841 gaacatcgac tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg
      901 aattcagcgc tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga
      961 ataattcagg gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt
     1021 cgaattcatc tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg
     1081 ctagataagc tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt
     1141 ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg
     1201 catcgaaatc gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg
     1261 tttctagaga tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca
     1321 tctcacggat cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat
     1381 cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc
     1441 ttgtggtaat gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc
     1501 caggcaagtg tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca
     1561 gcatcgccta attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct
     1621 acagttaaat gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag
     1681 gcacttgccg tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg
     1741 tcttttacat cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc
     1801 ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc
     1861 acttgacggt ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc
     1921 catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc
     1981 gcgagcagtc gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc
     2041 tctgacggct accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc
     2101 caaattgaat gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa
     2161 tccatcaatt gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg
     2221 gatattgtcc ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc
     2281 ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta
     2341 taattcgaca aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca
     2401 ttttcgttta ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa
     2461 agcaaagaaa tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc
     2521 accatctact acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg
     2581 attgaagaca cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt
     2641 aatctttttc gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt
     2701 caagacagca cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc
     2761 catgtaagag agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc
     2821 tacccaattc aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc
     2881 atggatgaac gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc
     2941 taacgtatgg acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc
     3001 ctggagtact tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc
     3061 atcattgacg tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat
     3121 ccattcaact tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt
     3181 gctgttaaga cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc
     3241 aatcctctac tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca
     3301 tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct
     3361 ttatcagaaa gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc
     3421 aagatgtggc cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc
     3481 aaaatatgcc cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt
     3541 tgtctgcccg agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa
     3601 tctgagtcaa agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca
     3661 gaagcatctc gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat
     3721 tgatctacgg ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata
     3781 ttacctgatg attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat
     3841 tcactatgtt catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca
     3901 tcttgaatga ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg
     3961 aaaataccgc tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt
     4021 actgcttttt tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac
     4081 aagtcggtaa aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc
     4141 catgaacctt gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg
     4201 cctgtttcac tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc
     4261 acccgatcga tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag
     4321 gttctcatcc aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa
     4381 tcgagccagt ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg
     4441 attcctaatt ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca
     4501 gcattccctg tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata
     4561 tagttcaata atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt
     4621 tcatttactc ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg
     4681 tctaaaattg caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt
     4741 tctccgccag aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt
     4801 aaagccattt tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca
     4861 tcacgtttcg cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca
     4921 cttggatatt gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct
     4981 aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc
     5041 atgatagcgg cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt
     5101 tctccggttt tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca
     5161 tgtagatttt ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc
     5221 actcatttta gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc
     5281 ttttatttat tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga
     5341 aataagccat tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc
     5401 ctttactggt ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg
     5461 gtgaagcact tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc
     5521 aataatattc acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt
     5581 caaccctgcg ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg
     5641 gtacgtagaa gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt
     5701 ttcttcaata tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa
     5761 gccagcatct tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat
     5821 gattgttgca ccatctttta attcggaaac atctttgact tttttagagt acaagcccat
     5881 tggttcgatg tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt
     5941 gttcaaataa ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg
     6001 gttcggtaat acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa
     6061 taatggtttc acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa
     6121 tgtcgtgtcg tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc
     6181 taatcctaca gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta
     6241 catcccccta tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct
     6301 tgaacgataa atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta
     6361 ccacgctggt agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg
     6421 gccatcgcag taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg
     6481 ggaatacttt caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca
     6541 tctgctgctt ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca
     6601 taaaatggtg ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg
     6661 cctacgattg ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac
     6721 cggaaaatat tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt
     6781 ttgcgtccaa tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc
     6841 attgagatta atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg
     6901 atttgtttaa aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat
     6961 tcctactcct tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt
     7021 catcaagaca gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac
     7081 aacgttgaca tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt
     7141 tctttcgcta aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc
     7201 taatagttct gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac
     7261 ttcttttttc ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa
     7321 ctccatcaca gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa
     7381 gacgatggtc aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt
     7441 tgtttgcggg tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc
     7501 caatgctcgt gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc
     7561 ttgtccgcgt ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt
     7621 gctctttggt acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga
     7681 ccataacagg ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc
     7741 tttgtttttt aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag
     7801 ctcaagtcca ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat
     7861 tccataaata tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc
     7921 gtttttccca ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac
     7981 atctttctct tataattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg
     8041 acgaaagctt gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct
     8101 cttccagtac ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg
     8161 tagtaagttt gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt
     8221 tacccatttt cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca
     8281 aagctttctc gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca
     8341 acttttttca agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt
     8401 tgaaacttaa gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac
     8461 cgattttatc attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg
     8521 ctttttccgc ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg
     8581 gcagattggc aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac
     8641 agtattcttt tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg
     8701 ccattttata tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg
     8761 tcctctcgta agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc
     8821 gcttttgaag ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc
     8881 ccgcttgtgt tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc
     8941 gttgaggcct tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg
     9001 tctaaccacg ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac
     9061 aattttctta cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata
     9121 tcaacccgat accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca
     9181 atgggatccc tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga
     9241 acagcagcat catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga
     9301 aacacacccg aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac
     9361 cgataaagcc gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca
     9421 catagatatt gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa
     9481 gtccttgggc cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat
     9541 caatacgtgc aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt
     9601 cagtcgtaac caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg
     9661 cgatgatgcg ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga
     9721 caagcatcgt tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga
     9781 tcaagagctt cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat
     9841 tatgtttagt tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg
     9901 gctggaaagt aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat
     9961 tctgatcata gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag
    10021 gagtcagttt ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc
    10081 caactaaata ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat
    10141 catggctaag tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta
    10201 aaatcacgcc ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc
    10261 atcccttttc ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa
    10321 gtctttccat agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat
    10381 gcagtacaaa agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca
    10441 attatctacc agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga
    10501 aggatctctt cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa
    10561 taaaaatatc gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta
    10621 gacaaaatcg agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc
    10681 acgatcatct tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa
    10741 acaccagagt ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact
    10801 agctggtctt ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata
    10861 aaaccaaaat cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt
    10921 tcgatctcaa aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa
    10981 agatagacag caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca
    11041 ttgagcaatt tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat
    11101 aataagtcgg cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg
    11161 atattcaatt cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac
    11221 agtacttctg ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat
    11281 aacttaaaca tggcaccact tccataactt tttatgataa ttactataaa aactatttga
    11341 tttaaatata caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa
    11401 ctagtaactt agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc
    11461 attttacaat gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt
    11521 atatatgaag aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt
    11581 agcttccctt ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg
    11641 cggtatctct atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca
    11701 cacgaaacaa acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc
    11761 actcatggtt atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt
    11821 catttggctg tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca
    11881 tgttatcaaa gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt
    11941 tggatttggg gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt
    12001 cttttggatt tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt
    12061 tttcgtaaga aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg
    12121 ttctctttgc ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt
    12181 agcattgttc gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt
    12241 gttcaagctt cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc
    12301 aacagctata tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt
    12361 ggatttctta aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc
    12421 tcattacgta cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa
    12481 aatcgcttaa taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga
    12541 acggccaaac aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg
    12601 caagcttttg aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata
    12661 agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag
    12721 aagctacttc tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga
    12781 cctcttgttc ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt
    12841 atttattgac gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa
    12901 aggattcagc ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct
    12961 tcagcaggct tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat
    13021 tgggtatttt attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat
    13081 ttgctgaaag caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc
    13141 aaacgattgc tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca
    13201 ctatcatcgg tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga
    13261 tgatggctgg aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca
    13321 tccatgcgaa agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg
    13381 tgatgatgtt gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa
    13441 aaagtgcact tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct
    13501 tacttgacaa ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc
    13561 tcgttgttgt tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg
    13621 aaacgccaac tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt
    13681 ttgtgatctt ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg
    13741 cacattttat cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag
    13801 actttgcaaa attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc
    13861 ttctccaaac agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg
    13921 aaaaaacagt tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg
    13981 cagatatcct tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc
    14041 catatatcct gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg
    14101 caatggattg gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc
    14161 aacggctact tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca
    14221 gacattttgc tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct
    14281 ttttcccgca aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc
    14341 tgattcgctt tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc
    14401 taaccataaa aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac
    14461 tggaacttcc tcctttttct ctatccaaaa ctacttgcca actaataaaa attgttctta
    14521 aaagtttgat aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac
    14581 ctgttctttt acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa
    14641 tgtaaaccat atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg
    14701 tcccatgttt taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca
    14761 aaggtgccca tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag
    14821 cagcaatcaa gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa
    14881 attttgggaa agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga
    14941 ccgtgatgga tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga
    15001 tcactacttc tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg
    15061 cgatacgcag tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag
    15121 aaatggtcga tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc
    15181 tagaattaac agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa
    15241 cagaatatat ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct
    15301 ggaaattatt caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga
    15361 ttgcttcatt tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta
    15421 ttttgcgggc attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct
    15481 tcctgttcat tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga
    15541 aaatcttctt agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga
    15601 agccaaatga attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa
    15661 atgaaaaagg actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc
    15721 cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg
    15781 taaattcctt cttatttctt gaagctgacc acttctatca caacctcttt catatcttat
    15841 tacttcttca ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct
    15901 tgtgcatatt ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct
    15961 tttttatcaa gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt
    16021 ttaccttcga tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt
    16081 ggatcttttc ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca
    16141 cgtgtgtttt tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag
    16201 aaatcttgat attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt
    16261 tcgattccat tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct
    16321 ttttcccagc tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat
    16381 tgcttacctt taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg
    16441 attagtttct cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt
    16501 tgaccaagca agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg
    16561 gcgaaatcgg gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca
    16621 gtttcgcctt tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta
    16681 ccaaattcaa ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa
    16741 aaacgagaga caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt
    16801 gctctgtttt cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact
    16861 tttgtctgtt ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg
    16921 ctgactgctt gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg
    16981 tctgtacgcg gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat
    17041 gttttctttg cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca
    17101 taaggcaatg gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg
    17161 cttgtactga tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga
    17221 tttttttgaa gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt
    17281 tgtggacgga actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc
    17341 tgcacacggc ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc
    17401 aatcctacca gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat
    17461 tctttcgcag gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag
    17521 atccataacc gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca
    17581 agttcccctt ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga
    17641 gccagctgct tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt
    17701 tttttaggga tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct
    17761 tccggcattt tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct
    17821 tcataataat ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg
    17881 cttggttttt ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat
    17941 gggctgatga tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc
    18001 ttcacaccag cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt
    18061 gatatattca taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc
    18121 ttcgaaagac tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca
    18181 ccaaggctca ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc
    18241 agcttttcta gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa
    18301 aagggtcatt taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa
    18361 gtagcgataa agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct
    18421 tttgtaaagt atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc
    18481 ctgcttcgat gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg
    18541 atctgattcg tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg
    18601 atcagtaact catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag
    18661 atctgcagtg cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt
    18721 tcctgcagaa gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta
    18781 tacagcgagg gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga
    18841 acatccatct cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt
    18901 ctttttttca tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca
    18961 cgaaaaagaa agagaaaaaa gagactttca ttgataaact acatattgta gtttataata
    19021 aagagctgta gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga
    19081 aaaattattt aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac
    19141 atttatggct ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac
    19201 gttaggtaat tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac
    19261 tttcttggtg actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag
    19321 aaaattgatg ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg
    19381 cgtgactagt gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta
    19441 tatcagtaat gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt
    19501 tcttggaacc ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg
    19561 gatctctgct tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt
    19621 tttcgtcttc ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga
    19681 aaacatggtt tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg
    19741 tatgttcgtc acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag
    19801 tctgtacatt cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat
    19861 tgcttcgata cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga
    19921 caagattggg agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat
    19981 cccaatggca ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat
    20041 ctctgatgct tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca
    20101 agctgctgga cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg
    20161 gcctatgatc ggctccagtg tttccagtgc atttggttat cgcggcgtct ttctttcgac
    20221 ttctcttctt gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca
    20281 gcaagaaact gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc
    20341 tcatagacaa taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg
    20401 cagtgttttt gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc
    20461 caaggtttct tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact
    20521 tcctggtact aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc
    20581 ttcttcgatg gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac
    20641 tttcggttca ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt
    20701 gtttcgcgcc aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag
    20761 gactccgccg caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg
    20821 tccagtagct gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt
    20881 ggttgctata cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa
    20941 aggcgcatat acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat
    21001 tttttctaaa gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca
    21061 gatgattttt gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc
    21121 ttcttcttcc ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata
    21181 taattgctga taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta
    21241 tagagctaaa agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac
    21301 ggtttgataa agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata
    21361 gtccaatggt tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc
    21421 aacgagttcg tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt
    21481 cttttcccat ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc
    21541 ccccttatat cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac
    21601 aaatcagccc aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag
    21661 cgatccagta gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa
    21721 atcctgctaa aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt
    21781 gttttttgat tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac
    21841 aaatcaaaat tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta
    21901 agaaagtcaa taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg
    21961 catttttttt attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca
    22021 tcgttgtttc tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca
    22081 agccgatatt gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca
    22141 tagcaggttg tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg
    22201 aagcaaggat atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt
    22261 ctgaacgttt cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc
    22321 acatcataac catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc
    22381 cattcactag attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa
    22441 atgcgataaa tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga
    22501 ttgtattgat gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa
    22561 taaaaatact tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa
    22621 attctcccat ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca
    22681 accagtaacc ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac
    22741 cgaaaatacc accatctaga tctggacgct tttctcctaa atgattcagc gataatacca
    22801 aagctaaaat accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag
    22861 aactagccag atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca
    22921 gagcaaccag tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca
    22981 cttataaaat tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc
    23041 ttaacaaaaa gcaactttat tcatattatt ggatattatg aactttttac acaagtattt
    23101 caagtataaa atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag
    23161 aaaaggtgtt tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta
    23221 ttgtaaaact tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat
    23281 tccatcataa aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac
    23341 gacttctgca cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata
    23401 acggaatttc gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat
    23461 ttcacttgca tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata
    23521 taatgtgttt gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc
    23581 gccaccgatc cctagtcctt gacgttgacc aatcgtataa tacatcaagc cagcatgttg
    23641 tcctttgact tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct
    23701 caaaaattgt ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt
    23761 agcagtcgca aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc
    23821 tagagggaac atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg
    23881 atctttatta ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac
    23941 ttgcgcataa tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa
    24001 tgctttgaat ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg
    24061 gtattcggct aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga
    24121 gtaataagga atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc
    24181 cgtgcataca ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc
    24241 gtatccttgt tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc
    24301 tacgacaacg cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc
    24361 tgaaacatct acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt
    24421 ctgaaaattt catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca
    24481 gtacacaaac atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa
    24541 attggcatac tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt
    24601 gaaaaactat ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc
    24661 ttttcttttt agatgttatg agttgctcag ataagtgatt ttgtttactc taagaaaaaa
    24721 gggaggcaaa agatacatga attactttat cagtgatatg cactttttcc atgaacgctt
    24781 attaggaaaa aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca
    24841 gttgatcact agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga
    24901 ccttgctatg catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca
    24961 actgcaagga acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta
    25021 tttagaaaaa aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt
    25081 aggtgccatc gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct
    25141 cgggcctaac gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat
    25201 cgagaacgat gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact
    25261 accatttggc actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa
    25321 gtacgatagg tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac
    25381 tcatattttt catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt
    25441 gtaacaagac ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc
    25501 ttttaccagt tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa
    25561 aacgattccg cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa
    25621 acgatcagct aatggtcctg caaataataa tccaattgga cctgccaggt tcatcaaaga
    25681 gttgaagttc cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc
    25741 catcagcagt gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa
    25801 gtacaagaac ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat
    25861 aatgatgtag gctataataa taggtttcat tcggtctttc catgttccta taaaactgat
    25921 cagtgcaccg cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc
    25981 aacggaacca ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa
    26041 caacagcatc acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa
    26101 ttgctgcagc ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg
    26161 agaaactttt gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac
    26221 atctaataaa ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg
    26281 cgcaatgatg aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg
    26341 agaagatgga acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc
    26401 gaccgaacgc ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt
    26461 tccgactact gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag
    26521 agctttttta tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg
    26581 aatcattcct agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa
    26641 ataccagatg atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga
    26701 taaaaagaac aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat
    26761 aaacatccct cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga
    26821 ttcttttttc aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt
    26881 acttagaaac aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat
    26941 aaaaaagaca actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt
    27001 caaaactagt tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct
    27061 aatcatccca tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt
    27121 gaaaatattg actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat
    27181 atcttttgat acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa
    27241 tggtcggatc aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt
    27301 atatcttttt gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt
    27361 tgtttcaaat gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt
    27421 atcctgattt ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag
    27481 acatctgttc atataagaat gacttcacta gttatttttt gacagcacga actaattgat
    27541 caaggaaata ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt
    27601 cttttagtat cggtgtatct tctccataca ttgcttctaa tacgtgtgaa ggctcgatat
    27661 ttcctgctgt acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca
    27721 gtagcaaatc atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg
    27781 ttaaatcacc atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt
    27841 gctggaatcc tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa
    27901 ctgcttttgc cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt
    27961 cctgctcccc gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac
    28021 cgacgccttt tggcccattg attttatgag cagagatact taataaatcg attcctaatt
    28081 cttctggatg gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg
    28141 gatgatccgc taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt
    28201 tcccgtacat cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga
    28261 tcgacagatt ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta
    28321 aatatttcat ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc
    28381 cttctttttg acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc
    28441 cactgttaaa gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg
    28501 ctgtttccaa tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa
    28561 acgtgtcttg catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg
    28621 ccgcatgatc cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca
    28681 cattctagca taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt
    28741 tcatgggcat agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct
    28801 cacttctgtc acaacttctt tccctctttt ttttgtttat tcgaattctt tttcttccag
    28861 acggaataac ggactcatcg gtgtattttc atggatgcgt ttgaccgctt cacccattaa
    28921 ttctgaacat gtaacgattg ttaagttttc tggatgacga ttttcttctg tgaaaacaga
    28981 atcagtgata caaatatctt tgattggtgc attgtctaaa atacttttcg catgatcgga
    29041 caataatcca tgtgacgcac aggcatatac ttcttttgcg ccgttttctt ttaatacatt
    29101 tgctgctcgt gcaagcgttg ctcctgtgtt caagatatca tctaccatga tacatgtctt
    29161 tccttgaaca tcaccgatca cataaccacc ctcagttgaa gcatcatctg catgatctac
    29221 gatggctaat gtgctgttca aatattctga aagactacgt gcacgctgaa caccgctgtt
    29281 ttttggcgaa acaactacga tgtcatctcc cgtcagacct aattgacggt aataatgagc
    29341 aaacaacggc atcgtaaata aattatctac aggaatatca aagaatcctt gtacttgaac
    29401 cgtgtgcaga tctaaagtca gtactcgagt tgcccctgct tcttcgatca agttagcgat
    29461 caatttcgca gtgatcggtt catgaggttt cgccgtgcgg tcttgcctag catacccata
    29521 gtaaggcaat acgacattga tcgttttcgc acttgctcgc ttcattgcat cgatcatgat
    29581 cagtagttcc atatagtaat cattgacagg tgcattcgtt gattgaacga tatacacatg
    29641 atcccctcga atactttctt cgatattgat ggaaatctcg ccatcactaa attgcttcac
    29701 gacgctttta cctaattctg ttccgaacac tttcgcgatt ttttcagcta atggacggtt
    29761 gccattcaaa ctaaaaatct tcaacgtgtc atcctgatac ttattactca tgtagtcctc
    29821 cccaaaactt ctctattctt cgatagttta acatattttt ccaagatttt ctgttaattt
    29881 cagcaaattc ttaaggaagt gaaactgttc cgttactcca actggcagga atgactttat
    29941 gataatcgcc ccagttgtcg tgtactttgt aataacgttc tcctgtttca gtttcaaaat
    30001 aagcgtacgc tgtcacccaa tgattgccat atgtactgcc aagtactttc aaaataccaa
    30061 tcatcacagg tttgccttca cgtatacgtt ttgtagctcg ctgccatgaa cctagaagag
    30121 ttgctcgtgc acgctcatga ttgccattct tctttaaaaa actagaaatc cctgtactaa
    30181 cctgaaaagg aatcgtagga agtcctagtg gttggatctg gctacgcaat gccttcgtca
    30241 aaaccaacga atcagccgta tttttcttgc gaatctcatt tggaagcatc caccccttac
    30301 gatagtcttg ataataggca aggagaactg ctgccgcgta agtaccacaa agatagcctg
    30361 agggggtagc ccatttctta tacctcaaaa aacgttcttc tggcaatccc acccagcaat
    30421 ttgcgagttc ctttcctgac cttttttctt cgatcaaaac gggaatatct tcttttctgt
    30481 tgatccatct agataactcg attggctctt cattgatcag cttccatttt ttacaagaaa
    30541 gaccctcttg ataagtatat tttgctggag aatattctac cggatgtcct gattgatcga
    30601 taatcacata actttgatct tcaaaaacca gagctaaaaa aagcaattta ctttttcttg
    30661 actgcagctg ctgagtgctg gctaattttt taattttttt atctactttg aaatataaag
    30721 gtttatccac tacttgattc agttgggatc gcctcctttt tttcattata agtgaaggat
    30781 tgaaaaaaac aaatcagttt gattgttcga attttcaatt ttttatgttc tatttgttaa
    30841 ttccttcatt ttgtaagata atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa
    30901 caaagaacga ttgaaacaag atttctatga agcagtcaat gaagaatggc aaaaaacagc
    30961 aaaaatccct gcagataaac cagctacagg cggtttccaa gatttagttg acgggatcga
    31021 ccaacttttg atgggagaaa cagacgatct gcttgcccat ccagaaaaag tgtcttcaga
    31081 aaaaatgaaa catttccttg catattatca actggcaaac gattatgacc gaagaaatga
    31141 gttgcatgct caaccgcttc aacctataat cgaaaaagtt gaatcattgc agtctttcga
    31201 cgaattgaat aagcaatttc cagaatggct gttggataca ttaccattgc catttaatct
    31261 ggatgtagat gcagatatga agaatgcgca aacgaacgcc ttctttgctt atccgccttc
    31321 tttattcctt ccagataaga cgtattatgc atcagaccat cccaacggtg cacaactaat
    31381 aggcgttttc tttgatatgg ctgttcagtt attagagctt gccggcaaag aaaaaagtca
    31441 ggcagaagca atcacggaac aagcagtgca atttgataaa ttgattgctc ctcatgtaaa
    31501 aagtgcagaa gaaaacgcag attacagcaa aatgtataat ccgcaatctt ttgatacatt
    31561 tattaaatac acagataaac tggacttgaa aaaacttgtc acgggtttga ttgggacaac
    31621 tccagataaa gtgatcgtaa ctgatccagt gtacttcgat catttgaatg acatcctgac
    31681 agaagctcat tttccgttat tcaaaagctg gatgatcatt aaaacagtac gttctcttag
    31741 tggttatcta tcagaagaat tccgccaagt cagcggaatc tattctcgcg tattgtctgg
    31801 aacagacgaa gcaatgcctc agaaaaaagc agcgtattac cttgcctctg gacaattcga
    31861 tcaagtagtt ggcgattact acggaaaaaa atattttggc gaagctgcta aaaaagacgt
    31921 agaacaaatg gtgaaaaaaa tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg
    31981 gcttggggat gcaacaaaag aaaaagccgt cataaaatta aataacttag gtatccaagt
    32041 aggctacccc gataagatcc cagcattatt cgaccagttc aaaacagtac ctgcagatca
    32101 gggtggtact ttgctttcaa atactcttgt tttcagcaaa ctcacattaa aagatcgttt
    32161 cagcaaatgg aacaaaccag tggatcgttc agaatgggaa atgagtgctg acacggtcaa
    32221 tgcttattat catccatttc ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc
    32281 attttacagt ctagaacaat caagctctgc taattatggt ggaatcggag ctgttatcgc
    32341 tcatgagatt tcccatgcat ttgataataa cggcgcttta tttgacgaat atggcaactt
    32401 gaacaactgg tggacagaag aagatcttac acaatttcaa aacaaagcac aagcaatgat
    32461 cgaagaattc gatggtctgc cttttgctga cggaaaagta aacggaaaat taaccgtttc
    32521 ggaaaatatt gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc
    32581 agatctttct atcgaggaat tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa
    32641 aaaagaatac caacaacttt tactgcaaat cgatgtccat gcccctgcta aactccgagc
    32701 aaacgtgcaa ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca cggaacaaga
    32761 cgctatgtat ctagcgccag aaaaaagagt gcgaatctgg taagaggttg tgccagaagt
    32821 ggtcagcttc aagaaataag aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat
    32881 ttcggcttat ttttcgaaga agttacttct ggaataccat gtattagagg ttgtgaaaaa
    32941 gctgcttagt ttcaagtagt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
    33001 aaatttgact tactaccgag aaactggctt ttgaacaccg tttatcgaga ggttgtgaaa
    33061 tcagcgtcct gacctgagta ttagagaaaa aaacggaaaa atagttcctc atattttgcc
    33121 gtttgttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gagattgtga
    33181 aaaagctgtc ctgcatcaag taataagaac agccaatcaa aagcagcaag gagaacctca
    33241 tctataaaaa gatagaagga agtgagacat ctattggtct catttccttc tatttttact
    33301 ttcctcagtc aattgtttta catcataaaa ggagaaattc cacttatttt tttcgctgtt
    33361 tcatgacata aacaactaca ccgattaaca aaattcctcc agctaatgct agcaacgggt
    33421 tattaaattc tcccgttttt gggagtcctt gagtgccatt tttttcactt tcttttgctg
    33481 tcctatccgg atgagaaggt tttccatttg gttttccatt tggtgattca tttcctggat
    33541 tttctctttc tggtttgtca tttcccgggg gcacatcggc agtttctcgc acagtgatcg
    33601 ctttttgctg ggtaacagat tctgtatgag cagaccattt ttccccatca aatatttccg
    33661 aacgataagt gacttctagc gtatattctc cttttgaagg tgctgaaaaa atagcggtat
    33721 gattagaaaa ttgagcactt gactgtgttt caacgatttg gatcttttct gggaaataac
    33781 gcatatcttc aaagacaggc gttccttgat ttggtgtgaa agttgacaca aaaactgtaa
    33841 aaggcttcag ttcaatgata tttccttctg cattctccgc cacaccattt gcttcttgta
    33901 taataatcgg attattttgt tcatttggtt ttgtctgatt tggttcttgt acgattgcat
    33961 actctggata ttccgaagac ggtgaggttt ctccgtcaaa aatagcacct acagataatg
    34021 gaagaccatc cactctttcc ttttctgttt gacgattccc cggtttaatc tgcatcagtc
    34081 catgaacatg gatacttcca ttttcatagc gcgtaacagg ctgctgacct tctttcgaac
    34141 atgtatttag agactcgatc caatcttttg atatggtatg tccaagagta ttttctgatt
    34201 gtttctctcg ttcattccaa tagtagttca acgagtggta gatttgatct tcgttttgat
    34261 tcaatgggcg gatctgctct ctcatctcaa gattctctgt tcggaaagat aagataccag
    34321 aagcatggta gtctgtgttt cctgctgaag aggtataaag agccacatta tatgaaccgt
    34381 tattaaaact tgtactgcta ttgacgataa tatctgttcc gctattggaa tcgattcctt
    34441 tcgcaagatt ttcgaaagac aaactattga tcaattcatg agggcccgtt aaagaagaac
    34501 cgcccatttt gaatccatta ccgttacctt ctccctcaat tcctggtacc cagccgtttc
    34561 gataagcaac tgagtttttg atgattactt ttccgatcga acctgtttca tttttagcaa
    34621 aaaggtccca accgtcatct gcattatgat acgcgataca accgtcaaat acatttcctt
    34681 ctcctattgt cagtttggcc gcaaatccat cggcatcttc aaatcctgga tcggcatttt
    34741 caaaagatgt acagttcttg actaagttat gcgcgggcca tgcttcgaaa ggatcatttg
    34801 agctaccgct gatttggatt cctgtatttc cattccgata agcatgaatc tcttcgatca
    34861 aattatgatt tccagaaatc tgtaatcctt tttgcatagc tgcactattt gtaatgtcaa
    34921 accctcgtaa accccagtag tgactgaaca gtgtcatccc atttccagtt cctttaaaat
    34981 caagtacagg acgcttgttt tctggatttt tctctgcaat caactgaatc gggttttctc
    35041 ttgtcccgtt aacgcctttt ggaatcgtca atcctctttc gaaggaataa gttccaggcg
    35101 ccatgacgat tgtctgccca gcatatgcgt atctcaatgc ttgtgtcaga gataatggat
    35161 tttgtctgct tccgtttcct gcatctgacc cttctggtgt tacataaact gtcgaatatt
    35221 taggtttacg gtagtccact gtatgaagaa tctcttttgt ttcataacta gtcatctcta
    35281 cgtattcttc tggcggatag tttttatctg gcgtaaaact gaaagtaaat tcgttcgttc
    35341 cttccatcag ttttgtctga taatcaaatt cccgattcgc agtgatagcc aagttttttg
    35401 cgttcttaag cacctctcct gttgctttat ttttaattgt caataatccg tcagcatttg
    35461 ctctaaagga catagtatga tcttggtttc ctgaatgatt actagaggtc acagtgtaaa
    35521 tcggatcaat caagcgagta ggtctttctt gcccaggagg atctgtcgca ggatctgagt
    35581 aagcaacttc gattccttct accttgatcg tcatattcct tgctgcagca aatcctgcat
    35641 aaataaccga ttcatctgaa gcgtataatt tttcccagtc atacatgatg tcagaagcca
    35701 tttcttctcc tgtttcagga tcgtaataat atgcctctaa accagtattc gtttttttca
    35761 tctctacgtc cagtttagta atcgagttcg tggccggaaa cagattccca tgaccgtttc
    35821 cgaatatatt ataagaacct gtttcttttt ccaagaaccc tgctgaagtc tctaaaggtg
    35881 ttgtttccac tgagacactc ccaggagctg gcgcagcttg cggatcttca gatgatattc
    35941 ctgtgattgc ccgtgtcccc aatccaagtc gcatcgtata tttgctgccg ctcgtgttga
    36001 ccacttcttg tctatccgag tcccagacat attcgatccg actgcctagt atggcaaatg
    36061 aatttgaata aaatgcctgc ccaaacaaat gattttttgg tactgcgtct cttaccatta
    36121 aagcaaatcc ttcttgggca ttcgtatagg tccaagattc tatttcgatc gtcgcccgca
    36181 tcctaaagtt gatgtctttg gggattgctt gataataata ggtcattcca tctgatccgc
    36241 ttgactggaa ttttccgccg ttattcgtgg aagagagccg cacgccgtct tctaatggtt
    36301 caatggcata cctactagca cttgtgcttt ctccgaaaac aacccctttc cactcttcat
    36361 ttgcttcttc agattgcttt tttgtctttt cattttctga ttctttcatc ataggatcat
    36421 ttccttcttc cgtatccata tcttcactag ttcttggtat ttcctccatt gttgacatcc
    36481 cttcaatgtt cacgtgtgca ttttcttcgt tcacagactg ttgttcgtta gcagcaactt
    36541 caacgacaga aattaaaacc ggggtactga gcataaggag attcatcatt agcgcactgt
    36601 attttatacg tttttttaac aagtgtattc ctcccgtaac aaattagaaa gcgtttactt
    36661 aaagtattac ctaatttatt tttactgtca ataaaaaaca aaattataat aagtgtattt
    36721 tataaaaaga aaaaatataa cttaagttgt tgataataaa gagatttatt aacggaaaaa
    36781 gaataagttt ttttaaaata acgtataaaa aagagcataa aacaaagtcg tttgttttat
    36841 gctcttcttt tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa
    36901 tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga
    36961 aacttgccac ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg
    37021 ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc
    37081 gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta
    37141 tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca
    37201 attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa
    37261 aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa
    37321 acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca
    37381 gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct
    37441 tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc
    37501 tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat
    37561 tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc
    37621 ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg
    37681 cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc
    37741 ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat
    37801 tcctggtaat tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac
    37861 tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc
    37921 tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc
    37981 taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga
    38041 aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat
    38101 ttttacttct tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt
    38161 ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga
    38221 tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt
    38281 gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg
    38341 ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg
    38401 tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta
    38461 ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat
    38521 caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac
    38581 ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat
    38641 atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga
    38701 cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg
    38761 tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt
    38821 tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc
    38881 gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc
    38941 tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc
    39001 aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt
    39061 ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa accttagatt
    39121 ttctctaaat atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta
    39181 aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag
    39241 ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct
    39301 ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt gtagttttag
    39361 ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac
    39421 tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat
    39481 tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc
    39541 agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag
    39601 ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt
    39661 atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg
    39721 agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc
    39781 tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg
    39841 acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac
    39901 aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct
    39961 gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca
    40021 aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg
    40081 atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt
    40141 cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca
    40201 aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg
    40261 atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac
    40321 aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg
    40381 aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca
    40441 tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc
    40501 aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc
    40561 tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg
    40621 cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca
    40681 ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca
    40741 atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta
    40801 gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct
    40861 tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg
    40921 tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg
    40981 aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa
    41041 cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg
    41101 atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc
    41161 gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt
    41221 taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac
    41281 aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc
    41341 attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac
    41401 gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa
    41461 gatggagacg attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt
    41521 gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc
    41581 catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc
    41641 tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt
    41701 cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata
    41761 catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc
    41821 cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc
    41881 agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat
    41941 cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc
    42001 tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag
    42061 cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat
    42121 tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa
    42181 acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac
    42241 cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg
    42301 aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac
    42361 agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa
    42421 ttcaccaaat acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc
    42481 aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag
    42541 tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt
    42601 accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat
    42661 ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc
    42721 ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc
    42781 taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc
    42841 gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa
    42901 gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca
    42961 taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc
    43021 aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc
    43081 caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa
    43141 tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc
    43201 atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta
    43261 tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa
    43321 aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc
    43381 atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa
    43441 aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc
    43501 accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa
    43561 accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct
    43621 ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt
    43681 tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata
    43741 aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt
    43801 cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac
    43861 catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga
    43921 tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga
    43981 tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg aagataaaag
    44041 cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat
    44101 ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc
    44161 catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt
    44221 gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt
    44281 caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt
    44341 catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca
    44401 ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt
    44461 ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt
    44521 ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc
    44581 cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc
    44641 acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat
    44701 ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc
    44761 ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa
    44821 ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc
    44881 tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag
    44941 acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc
    45001 aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta
    45061 agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt
    45121 cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct
    45181 ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc
    45241 atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc
    45301 tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc
    45361 ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa
    45421 aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt
    45481 ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta
    45541 atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta
    45601 ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc
    45661 gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat aaagatggta
    45721 tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt
    45781 cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc
    45841 ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac
    45901 gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat
    45961 ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga
    46021 atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc
    46081 taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata
    46141 gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac
    46201 cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag
    46261 cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg
    46321 ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc
    46381 tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa
    46441 ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt tcattttctt
    46501 caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt
    46561 ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca
    46621 tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg
    46681 acccgcgttt catattcggt actctttctg gccctggatt ttctactcct acgatctgaa
    46741 tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg
    46801 gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc
    46861 cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg
    46921 cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa
    46981 atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt
    47041 cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg
    47101 gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt
    47161 tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta
    47221 aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt
    47281 cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc
    47341 cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac
    47401 caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga
    47461 cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt
    47521 ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc
    47581 taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat
    47641 acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca
    47701 ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat
    47761 gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt
    47821 ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa
    47881 tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa
    47941 tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca
    48001 aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt
    48061 caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac
    48121 cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga
    48181 ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc
    48241 ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca
    48301 gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc
    48361 gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt
    48421 ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca
    48481 gagactggaa ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat
    48541 tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt
    48601 gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa
    48661 ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca
    48721 tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta
    48781 gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca
    48841 gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag
    48901 tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg
    48961 gttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg
    49021 atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca
    49081 tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca
    49141 accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt
    49201 agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc
    49261 atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc
    49321 gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag
    49381 atagccaagc catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc
    49441 atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca
    49501 tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac
    49561 agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg
    49621 ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc
    49681 ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat
    49741 ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt
    49801 ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat
    49861 cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt
    49921 ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg
    49981 aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat
    50041 actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat
    50101 tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta
    50161 cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga
    50221 tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat
    50281 cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca
    50341 gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat
    50401 agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt
    50461 atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat
    50521 ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat
    50581 tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt
    50641 gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc
    50701 gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa
    50761 tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg
    50821 gctctccgat tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat
    50881 ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc
    50941 ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact
    51001 ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc atgtggattg
    51061 gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta
    51121 tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat
    51181 ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt
    51241 gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat
    51301 ctactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg
    51361 ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg
    51421 tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg
    51481 caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac
    51541 ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat
    51601 cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc
    51661 cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac
    51721 ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag
    51781 tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt
    51841 ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc
    51901 cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg
    51961 ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg
    52021 cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat
    52081 ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa
    52141 tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa
    52201 ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag
    52261 aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac
    52321 gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat
    52381 gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt
    52441 cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc
    52501 tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa
    52561 cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat
    52621 agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg
    52681 tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga
    52741 tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa
    52801 tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg
    52861 cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat
    52921 ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta
    52981 gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg
    53041 gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt
    53101 caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt
    53161 cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt
    53221 ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt
    53281 gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa
    53341 acttcgggta gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg
    53401 ggtcttgcgt agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa
    53461 tgcgctggag tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt
    53521 cttcgctacg ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt
    53581 ggttatctat tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg
    53641 ggatcttttc tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc
    53701 cgacatacca ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat
    53761 aggaggatag acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat
    53821 cgattgctaa acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc
    53881 attcaagggt ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta
    53941 agaatacctc atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat
    54001 gatagactga ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct
    54061 tcaattttaa ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa
    54121 caatcaacaa aacaatgccc atctaattat aatttaattt aattttaatt ttaaatcaaa
    54181 tgatatcctt tttagttaaa aaacaccaat tatacaacat ttattattaa aattaaatta
    54241 taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca
    54301 actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt
    54361 tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa
    54421 tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga
    54481 tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag
    54541 ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac
    54601 ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt
    54661 tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat
    54721 attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac
    54781 cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt
    54841 gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat
    54901 tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa
    54961 aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca
    55021 agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag
    55081 aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta
    55141 aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt
    55201 cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt
    55261 gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt
    55321 gaaccgagct ttttctcttc atttttttgt aatgataaat catatacaga actgttcgac
    55381 ttgcagttcc taatgtgaaa acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga
    55441 taagacgtgg aatccattca atgatttcta agaaataatc attggctttt ttaccaatcg
    55501 atcgattcat catccttttg aacctcctgt caaatccatc ccttcaatga aataacgttg
    55561 tgcaaaaaag aacagcaaaa tcgtcgggat caagacaagt gttgctcctg ccatcagata
    55621 attccattgt gtcgttgctt ggttctgaaa aacttgcaag ccaatctgta acgtgtattt
    55681 ttcttgatct tggatatata acagaggacc taagaaatca ttccaagctg cgttgaagga
    55741 attgatcgca atggtaatca aggcaggttt cgtcaatgga agcatcaaat gactccaaat
    55801 atacaaatga ttcgctccgt cgatttcagc agcttcgatc aattcattat tgatcgataa
    55861 gtaaaattga cgcatcagaa atatattgaa cgcattgccg aagaacgatg gaacgatcag
    55921 gggcaaataa gtacctaccc agccgatctt ggagaataat acatattgag ggatcatcgt
    55981 tacaaacccc gggatcatca tcgtagaaag aaccaaagca aacatcagct ttttcccagg
    56041 aaaatcaagc ttcgcaaatc cataagcaat aaaagaattt gaaaggacat taccgatcac
    56101 tactagcaca gtgatgaata atgtatttct tgcatagcgc caaaaaggaa aggcggcaat
    56161 cgtatccaga tagttttccc atacgatttt ttctgggaaa aaggtcggag gataacgaac
    56221 gatttcttcc attggtttca atgaagtaaa caccatccaa agcaatggaa tgatcaaaat
    56281 gattcctatt actgtcatga ggacataact tagtattttt ttgtttgccg attcacactg
    56341 gtctttttga aataatccat catttgccgt cacctccaga ataatagacc cacttaggtg
    56401 caagcttcaa attgacaaac gtcagaacca acacgatcag aaacaggatc attgacatag
    56461 ccatcgcata acccatatca aaagctacga acgccttgtt ccacatatgc agattataaa
    56521 aaagcaaaga atttgcaggt ccgcctgttc catttttcgt catgacataa gcttcttgaa
    56581 aaatttggaa agaaccgatc gtagaggtga tcacatcgta aaaaataata ggagtgatta
    56641 atggcaaagt aatacttttg aactttcccc aaacgcctgc tccatcgatt tctgctgatt
    56701 catataaaga tctaggaaca ttttgtaatg tagctaagta aagtagcatt gctcctccag
    56761 aactccatat tttcatcaaa atcaacgcag gtttcgtcca ttgtggatca aacagccaag
    56821 ctggtccagg gatatggaac cagcccagaa atgtgttgat caatcctgca gatggagaaa
    56881 gcagctgcat ccataagatg tagactgcta ctcctgataa gactgccggt aaatagaaag
    56941 ctgtacggaa tacactgatt cctttgattt tttgattgag tactgtggct aaaaacacac
    57001 tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata aacggtgttg tataacgatt
    57061 tccaaaataa atcatcgtga acgaacattc ttttgaaatt tgctagacca atgaaattca
    57121 tcttggatgt caaattataa tcggtgaaac taccaataac tgaaaaaagc ataggcccta
    57181 gcataaatac caagaacccg ataataaaag gcgagataaa taaataggcc catcttgctt
    57241 cttttttctt tcggatagat aaacgcttca aattttcttg cctcctctgt acctagacag
    57301 tcaaaaatgg attattgatt ttgcttgact aagttttcta ctgctttttg tgcatctgcc
    57361 aatccttctt ttggtgattt tgttcctaaa agaatctggt caatctgttc attcactaat
    57421 gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca ccgtattttt aaagttttcg
    57481 tccgccattt gatagatcat ttttccttta tccgttaggt tgtcattatt cactaaattc
    57541 tcattggctg tcttgctcgc catgatatca aaactttttt cgccgaattt ttcttgtact
    57601 tctggagaag aaagatattt gatgaattca taagaggcct caggatcttt tgctccgtat
    57661 ggtacttcca agacgaatcc gccaccccac gaccaatggc ctgaaccttc ttctttttct
    57721 gggagctgca tcacgccaaa atcaaaatca tccggtgcat tttccatcaa actagagtaa
    57781 taattgatat tttgggcacg catggctact aatccggaaa taaatggatc agatacacca
    57841 gacccaaatt cggcttctaa tttattgatc gtatttttcc cataataatc ttgccagtct
    57901 tggatccact ccaaggcttc cactttattt ttagtatcaa tcttgacttt ttcatctttg
    57961 tcaaaccaac tagtcccgtt atctgcgttt agcgcccata catcagcccc taaattccat
    58021 aatggataaa aaccgatgcg tgtaaattct tttccttctt ttttatctag tttacgtgct
    58081 gtttcttcta attcatccca agttgtcggt agatcttgtt cactgattcc agcttcttta
    58141 aatatctttt tattataaaa aatgacttgt gtatctgtat tgaatgggat cgcataagga
    58201 tcaccttcat attctactgt atcccaaagc tgcggataaa aatccttaga agtgtctttt
    58261 tctacatatt tggagagatt agttgcttgc tgtgcttctg ctctttgggc aacggaattt
    58321 atgtcctgta caataacatc gggtgcattt ccagctgtta ttgcagataa agatttagtc
    58381 cagatgtcac cccatggctg atacttataa tttacatgga tctcgttttg tgattgattg
    58441 aaatcctcaa tgatttcttc gatagcttct tttcgtgcac cggatcccca aaaggaccag
    58501 aaatcaatat tttttcttcc atcacttgtt ttttcagaag aatttgctgc acttgaactg
    58561 gcatcgcttg cattacttga tccgcatcct cctaataaaa acaatgctga acttgccagc
    58621 aaccctgtaa acaataattt tttcatattt tacctccaaa ttttaatgta accgatgccg
    58681 gttttaatat aacgatatca ttttgttctg aaaataacaa tttataaggt atgattctaa
    58741 aaaaattatt aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt
    58801 aatctttcgt agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta
    58861 gacaccagaa agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga
    58921 aatatctgca ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc
    58981 tgttccaagg ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg
    59041 caacagaaga aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg
    59101 gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta
    59161 tctctggaat gaatccacag cacgcgatcg tagctggctt aggacctcgt cctgctgaca
    59221 gcgtgggcaa tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact
    59281 ttagagctaa tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa
    59341 tgatcgatga tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc
    59401 atcaaaatct atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag
    59461 tcccaagtac tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact
    59521 tctcagaggg agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg
    59581 aaggcgaata tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac
    59641 cagtaacacc aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa
    59701 acacccgttt tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa
    59761 ccacccttca aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct
    59821 aaagacgctg gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac
    59881 ctcttaaaag gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca
    59941 ctcagcttat ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc
    60001 gcttcatttt gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca
    60061 aatttatatt ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg
    60121 attataaatt atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta
    60181 ctttgtacgg gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta
    60241 tttcaatcgc tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct
    60301 tcactgacag aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca
    60361 atctatttta tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata
    60421 aagccgtata aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc
    60481 cgataacact agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga
    60541 atagaactag tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat
    60601 tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc
    60661 cctacaaacc aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc
    60721 taaattccat ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata
    60781 cataaccttg atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg
    60841 agtactacct aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac
    60901 tcttagtaaa gtatctgaac tattaatttt taaactatca tttttatcct caactacatg
    60961 cacatctgtt gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat
    61021 tgcaatatct tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta
    61081 taaaataac
//
LOCUS       DAFCHQ010000008        56222 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000008 DAFCHQ010000000
VERSION     DAFCHQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.008"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            62..292
                     /locus_tag="LNC71_000564"
     CDS             62..292
                     /locus_tag="LNC71_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK6465660.1"
                     /translation="MGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(496..861)
                     /locus_tag="LNC71_000565"
     CDS             complement(496..861)
                     /locus_tag="LNC71_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465661.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1069..1551
                     /locus_tag="LNC71_000566"
     CDS             1069..1551
                     /locus_tag="LNC71_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465662.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1748..2638
                     /locus_tag="LNC71_000567"
     CDS             1748..2638
                     /locus_tag="LNC71_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBK6465663.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2963..5434
                     /locus_tag="LNC71_000568"
     CDS             2963..5434
                     /locus_tag="LNC71_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6465664.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5537..7273)
                     /locus_tag="LNC71_000569"
     CDS             complement(5537..7273)
                     /locus_tag="LNC71_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBK6465665.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7270..7974)
                     /locus_tag="LNC71_000570"
     CDS             complement(7270..7974)
                     /locus_tag="LNC71_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6465666.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8105..8608
                     /locus_tag="LNC71_000571"
     CDS             8105..8608
                     /locus_tag="LNC71_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBK6465667.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8568..8906
                     /locus_tag="LNC71_000572"
     CDS             8568..8906
                     /locus_tag="LNC71_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBK6465668.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8927..10345
                     /gene="ffh"
                     /locus_tag="LNC71_000573"
     CDS             8927..10345
                     /gene="ffh"
                     /locus_tag="LNC71_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBK6465669.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10457..11035
                     /locus_tag="LNC71_000574"
     CDS             10457..11035
                     /locus_tag="LNC71_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBK6465670.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11039..11560
                     /locus_tag="LNC71_000575"
     CDS             11039..11560
                     /locus_tag="LNC71_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6465671.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11639..12193)
                     /locus_tag="LNC71_000576"
     CDS             complement(11639..12193)
                     /locus_tag="LNC71_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6465672.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12446..12721
                     /gene="rpsP"
                     /locus_tag="LNC71_000577"
     CDS             12446..12721
                     /gene="rpsP"
                     /locus_tag="LNC71_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBK6465673.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12733..12984
                     /locus_tag="LNC71_000578"
     CDS             12733..12984
                     /locus_tag="LNC71_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBK6465674.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13109..13900
                     /locus_tag="LNC71_000579"
     CDS             13109..13900
                     /locus_tag="LNC71_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465675.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14070..14591
                     /gene="rimM"
                     /locus_tag="LNC71_000580"
     CDS             14070..14591
                     /gene="rimM"
                     /locus_tag="LNC71_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBK6465676.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14581..15342
                     /gene="trmD"
                     /locus_tag="LNC71_000581"
     CDS             14581..15342
                     /gene="trmD"
                     /locus_tag="LNC71_000581"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBK6465677.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15478..15825
                     /gene="rplS"
                     /locus_tag="LNC71_000582"
     CDS             15478..15825
                     /gene="rplS"
                     /locus_tag="LNC71_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBK6465678.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15957..17093)
                     /locus_tag="LNC71_000583"
     CDS             complement(15957..17093)
                     /locus_tag="LNC71_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6465679.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(17209..17775)
                     /locus_tag="LNC71_000584"
     CDS             complement(17209..17775)
                     /locus_tag="LNC71_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HBK6465680.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(17911..18564)
                     /locus_tag="LNC71_000585"
     CDS             complement(17911..18564)
                     /locus_tag="LNC71_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465681.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18614..19042)
                     /locus_tag="LNC71_000586"
     CDS             complement(18614..19042)
                     /locus_tag="LNC71_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK6465682.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(19047..19421)
                     /locus_tag="LNC71_000587"
     CDS             complement(19047..19421)
                     /locus_tag="LNC71_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6465683.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            19725..19910
                     /locus_tag="LNC71_000588"
     CDS             19725..19910
                     /locus_tag="LNC71_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6465684.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            19923..20171
                     /locus_tag="LNC71_000589"
     CDS             19923..20171
                     /locus_tag="LNC71_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465685.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            20202..20372
                     /locus_tag="LNC71_000590"
     CDS             20202..20372
                     /locus_tag="LNC71_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465686.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(20474..20932)
                     /locus_tag="LNC71_000591"
     CDS             complement(20474..20932)
                     /locus_tag="LNC71_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465687.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            21100..21417
                     /locus_tag="LNC71_000592"
     CDS             21100..21417
                     /locus_tag="LNC71_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465688.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            21350..21829
                     /locus_tag="LNC71_000593"
     CDS             21350..21829
                     /locus_tag="LNC71_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBK6465689.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            21846..22022
                     /locus_tag="LNC71_000594"
     CDS             21846..22022
                     /locus_tag="LNC71_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465690.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            22279..22710
                     /locus_tag="LNC71_000595"
     CDS             22279..22710
                     /locus_tag="LNC71_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465691.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            22716..23033
                     /locus_tag="LNC71_000596"
     CDS             22716..23033
                     /locus_tag="LNC71_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HBK6465692.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            23152..23838
                     /locus_tag="LNC71_000597"
     CDS             23152..23838
                     /locus_tag="LNC71_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6465693.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            23813..25162
                     /locus_tag="LNC71_000598"
     CDS             23813..25162
                     /locus_tag="LNC71_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6465694.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            25153..25626
                     /locus_tag="LNC71_000599"
     CDS             25153..25626
                     /locus_tag="LNC71_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6465695.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            25601..26128
                     /locus_tag="LNC71_000600"
     CDS             25601..26128
                     /locus_tag="LNC71_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HBK6465696.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            26143..28455
                     /locus_tag="LNC71_000601"
     CDS             26143..28455
                     /locus_tag="LNC71_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBK6465697.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            28702..29019
                     /locus_tag="LNC71_000602"
     CDS             28702..29019
                     /locus_tag="LNC71_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBK6465698.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            29016..29177
                     /locus_tag="LNC71_000603"
     CDS             29016..29177
                     /locus_tag="LNC71_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6465699.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            29180..29479
                     /locus_tag="LNC71_000604"
     CDS             29180..29479
                     /locus_tag="LNC71_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465700.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            29479..29793
                     /locus_tag="LNC71_000605"
     CDS             29479..29793
                     /locus_tag="LNC71_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBK6465701.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            29786..30316
                     /locus_tag="LNC71_000606"
     CDS             29786..30316
                     /locus_tag="LNC71_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBK6465702.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            30313..30582
                     /locus_tag="LNC71_000607"
     CDS             30313..30582
                     /locus_tag="LNC71_000607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465703.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            30512..30715
                     /locus_tag="LNC71_000608"
     CDS             30512..30715
                     /locus_tag="LNC71_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBK6465704.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            30712..30999
                     /locus_tag="LNC71_000609"
     CDS             30712..30999
                     /locus_tag="LNC71_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465705.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            31486..31899
                     /locus_tag="LNC71_000610"
     CDS             31486..31899
                     /locus_tag="LNC71_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBK6465706.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            32519..32683
                     /locus_tag="LNC71_000611"
     CDS             32519..32683
                     /locus_tag="LNC71_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465707.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            33095..33640
                     /locus_tag="LNC71_000612"
     CDS             33095..33640
                     /locus_tag="LNC71_000612"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6465708.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            33641..33958
                     /locus_tag="LNC71_000613"
     CDS             33641..33958
                     /locus_tag="LNC71_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6465709.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(34115..34279)
                     /locus_tag="LNC71_000614"
     CDS             complement(34115..34279)
                     /locus_tag="LNC71_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HBK6465710.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            34381..34902
                     /locus_tag="LNC71_000615"
     CDS             34381..34902
                     /locus_tag="LNC71_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBK6465711.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            34889..35362
                     /locus_tag="LNC71_000616"
     CDS             34889..35362
                     /locus_tag="LNC71_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBK6465712.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(35369..35527)
                     /locus_tag="LNC71_000617"
     CDS             complement(35369..35527)
                     /locus_tag="LNC71_000617"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBK6465713.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            35620..36381
                     /locus_tag="LNC71_000618"
     CDS             35620..36381
                     /locus_tag="LNC71_000618"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK6465714.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            36601..37920
                     /locus_tag="LNC71_000619"
     CDS             36601..37920
                     /locus_tag="LNC71_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK6465715.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            37936..39156
                     /locus_tag="LNC71_000620"
     CDS             37936..39156
                     /locus_tag="LNC71_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK6465716.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            39191..39868
                     /locus_tag="LNC71_000621"
     CDS             39191..39868
                     /locus_tag="LNC71_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBK6465717.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            39865..41058
                     /locus_tag="LNC71_000622"
     CDS             39865..41058
                     /locus_tag="LNC71_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK6465718.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            41073..41399
                     /locus_tag="LNC71_000623"
     CDS             41073..41399
                     /locus_tag="LNC71_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK6465719.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            41403..41738
                     /locus_tag="LNC71_000624"
     CDS             41403..41738
                     /locus_tag="LNC71_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK6465720.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            41735..42130
                     /locus_tag="LNC71_000625"
     CDS             41735..42130
                     /locus_tag="LNC71_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465721.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            42127..42498
                     /locus_tag="LNC71_000626"
     CDS             42127..42498
                     /locus_tag="LNC71_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465722.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            42514..43074
                     /locus_tag="LNC71_000627"
     CDS             42514..43074
                     /locus_tag="LNC71_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6465723.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            43199..43510
                     /locus_tag="LNC71_000628"
     CDS             43199..43510
                     /locus_tag="LNC71_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465724.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            43534..43725
                     /locus_tag="LNC71_000629"
     CDS             43534..43725
                     /locus_tag="LNC71_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465725.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            43725..47573
                     /locus_tag="LNC71_000630"
     CDS             43725..47573
                     /locus_tag="LNC71_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBK6465726.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            47574..48446
                     /locus_tag="LNC71_000631"
     CDS             47574..48446
                     /locus_tag="LNC71_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HBK6465727.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            48443..49492
                     /locus_tag="LNC71_000632"
     CDS             48443..49492
                     /locus_tag="LNC71_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465728.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            49496..50320
                     /locus_tag="LNC71_000633"
     CDS             49496..50320
                     /locus_tag="LNC71_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465729.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            50338..52398
                     /locus_tag="LNC71_000634"
     CDS             50338..52398
                     /locus_tag="LNC71_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBK6465730.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            52415..52564
                     /locus_tag="LNC71_000635"
     CDS             52415..52564
                     /locus_tag="LNC71_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465731.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            52561..53007
                     /locus_tag="LNC71_000636"
     CDS             52561..53007
                     /locus_tag="LNC71_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465732.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            53009..53146
                     /locus_tag="LNC71_000637"
     CDS             53009..53146
                     /locus_tag="LNC71_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBK6465733.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            53181..53423
                     /locus_tag="LNC71_000638"
     CDS             53181..53423
                     /locus_tag="LNC71_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBK6465734.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            53439..53636
                     /locus_tag="LNC71_000639"
     CDS             53439..53636
                     /locus_tag="LNC71_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK6465735.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            53647..54663
                     /locus_tag="LNC71_000640"
     CDS             53647..54663
                     /locus_tag="LNC71_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBK6465736.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            54778..55986
                     /locus_tag="LNC71_000641"
     CDS             54778..55986
                     /locus_tag="LNC71_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465737.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            55970..56140
                     /locus_tag="LNC71_000642"
     CDS             55970..56140
                     /locus_tag="LNC71_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465738.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT        19238 a         9561 c        11871 g        15552 t
ORIGIN      
        1 aatagtagcg atattattac ttctgaatat atggattttt gataggagta gaaatgcaag
       61 tataggtttt agaactaaac gaagtctgtc ttctaaaaaa aattgggtat actcacaaac
      121 tattttttat ggaggaattg ttttaattag tttactttct tcaacacttt attctttgaa
      181 tattatagat gtttccacct caaattcaat atctattatt ggaatcatta ttgctgcaat
      241 tattacacaa ctgtttttag tgtttggaga gaagaaaaga agtaaaaaat agaaaaacta
      301 gtgatagttt tggtgatagt gatattaaaa atcagaaaag atagaaaaag caggagatag
      361 ataaaatgct gatatttcag cattttttaa gagtataaaa gagaaaaaaa gtaataaatg
      421 tatacatgat ataagaaaaa catatatgta taaaaagaag aaagtagctt ctctttgtac
      481 atatatggtt tgtttttatg gatgccgagc ttttttataa gataaggcta ccgcccataa
      541 cgacccaagg atttgtggaa ctaagtaaat acctgcaaga agcaagatca gctgggtcgg
      601 ttttaaagga tgatagaagt aacgccattc agtaaaattg gcgcaaaata aataggaagt
      661 gacagtagat ataatattgg caatgaataa agatataata cgcctgttcc tttgaaaata
      721 atatccagca aaaagagata aaaatatcaa tgtgatgaac ccgcttacgg aatgataacg
      781 aaaatcaagc agtaaggcga aatgcacata aggaacaaaa gataaaagca acggaaggtt
      841 tttccagcaa gtttttatca tctttcatca ccacctaaca agtgttatta ttttgttagt
      901 tttattgtaa attgaatcct gcaataatca attacttttt cattatttct agattaaatg
      961 aataaactct catgccatta tctctaaaaa aaaagttttt ccccctaatt tttttagata
     1021 gaaaattgta tagtatgatg agagaaaaag tagtttgggg gaacgttaat gagaagaaaa
     1081 gcagtaatcg atcggcttta tttatttttt tggttttttc ctatcgtttt gctacctttt
     1141 acatgggtaa atgctgttgt acaaggtgcg gagatattag gcctgatcca aggaagcgga
     1201 tttgtggttt ctaggaattt tccagctttg actgttttct atttagtgat aagtgtaggt
     1261 ctcttattac caaaatctct gatcaatcac tacaattggc agctgcttta cttttcttgc
     1321 ttattagtat ttacatctat gttttctttg ctgcctcgct tgattatcgg ccctaatggt
     1381 ttgagcgaga gtggattgaa ggattcagct tattcctcca tgtatttctt gacgatcaaa
     1441 ccatggttct atttagtaat tgcctcattt attcttgcaa cattttttta cttctggaca
     1501 gaaatcagga taaaaagtga acgatctgta tctataaaaa ataactattg atggtggatc
     1561 atcagtaaaa aagcatatga gctgcgtaaa atgcatgttt ctcatatgct ttttttatta
     1621 ttatgtaaag aatgcgaaaa acgtttacat gatttttaca taacttaact atatttttac
     1681 agcaaccata cgagggattt acagtcgact gataaacttt agttgtaaat aagaaaggag
     1741 caataaaatg aaaaaagtat tgtttactct aggtatgatg ggaattcttt tagccggatg
     1801 cggctcagga tcaggaaata cagatggctc tgccacacaa aataattcaa gtgactcaaa
     1861 tgaacaagta aaaatcgttg cggtaggttc gacagcatta cagccgttag tcgatgcagc
     1921 acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag
     1981 cggaactggt ttgagccaag tcgaagctgg agcagttact atcggaaact ctgatgtatt
     2041 tgcagaagaa agagacggag ttgatgcttc aaaattagtg gatcataaag tagcagtggt
     2101 cgggatggcg ccaatcgtca ataaagatac agatgtcaaa gatatcacca aacaggaatt
     2161 gattgatatc tttacaggaa aaatcactaa ctggaaggaa gttggcggga aagatcaaaa
     2221 aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc gcgacattcg aaaaatgggg
     2281 actggatggt gctacccctg tgcagtccca agaacaagat tcatcaggaa cagttcggca
     2341 gctggtaagt caaacaccag gagcaatcag ctatttagct ttctcatatc tcgatgattc
     2401 cacacaagca ctaagtatcg acggtgtaga accaaaagaa gagaatgtag cagataacag
     2461 ctggggaatc tggtcatatg aacatatgta cacgaacgga aaaccttccc ctgaagtcca
     2521 aaaattctta gactacatga tgacagaaga aatccaagaa ggacctgtca aagaattggg
     2581 ctatttacca atcacaatga tggaagtaga acgtgatcac gaaggaaata tcaaataact
     2641 agcagataaa catgccacgg tagtcgttac tattaaggaa aaatcaacta tgtttcccac
     2701 atatacattt ttcctttaat tgtgaaaaat agttcctcat attttgccgt tttttgagct
     2761 aataccgagg gtcagctgat taaacaccgt ttattcgaaa aaaagagcta cgacatagaa
     2821 ctgtcgtagc tctttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg
     2881 aggaacactt tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa
     2941 cgaaatcaat cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa
     3001 agtaacacaa agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt
     3061 aacggcagat caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc
     3121 cacattcaaa acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat
     3181 ctttcttatc ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt
     3241 accagttatc atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat
     3301 cttggacgaa ctaaatgttt taaatacagc aaaagtcaat gtcatacggg acgggataga
     3361 acaaacaata gatatagaag accttgtaat agatgacatt gttattttag agactggaca
     3421 tcaaattcca gcagatgcag aggtcgtgga aggaaaactg caagttaatg aggctttatt
     3481 gacaggcgaa gcagatgaaa taaccaaaga aataggcgat ccattaatgt ccggcagttt
     3541 cattgtttcc ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc
     3601 ccagctgaca gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc
     3661 tcttaatcaa ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt
     3721 ttctcaaagt tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc
     3781 agcattgatc ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt
     3841 aagtgcgact aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac
     3901 attggcaagg gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat
     3961 gtctgttcaa aaagtgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt
     4021 ggaagcatgc attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc
     4081 tgttaaagca tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc
     4141 ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc
     4201 cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga
     4261 aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt
     4321 gacagaagca gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc
     4381 aagagccaca tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga
     4441 taatccgctc accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta
     4501 cgttgatgca tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt
     4561 ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa
     4621 tacagtagct atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg
     4681 cagtatagcg atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact
     4741 tgattctgac ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa
     4801 tattgagcga tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact
     4861 gttttcagtg atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag
     4921 tttgtttact atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat
     4981 cagagggaaa ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat
     5041 gatcgtagtc ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt
     5101 ttctacagca tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg
     5161 ttcaccgctg aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt
     5221 ttcggtcatc tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt
     5281 gctgttgagc attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt
     5341 agaatatttg tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa
     5401 aaaatttcta tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa
     5461 aaagaacaac cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac
     5521 gagcagttgc tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa
     5581 gatgactgtc tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta
     5641 accctaatcc ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct
     5701 caaagatccg ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg
     5761 atagatcatc tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga
     5821 cagcattttc aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg
     5881 gttcgttctt tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat
     5941 tttgaacgat ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct
     6001 cgccatcttt tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt
     6061 tttgcatgat ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta
     6121 aagtctcggt aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc
     6181 caacgaaatc cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc
     6241 ctagaatctg tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct
     6301 gccgaccttc tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat
     6361 gaatcagctg aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat
     6421 agcttaccac gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag
     6481 catcatcaat caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt
     6541 ctgtggtagt acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct
     6601 gatacaattc ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt
     6661 gcaccatttt ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca
     6721 gataatagat cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac
     6781 ggcgaaaact ctcagttctc ggtaaaaaac cgcttgtcgg ttctgcgata cgtaaaatgg
     6841 cttctaactg gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag
     6901 tagtactttt gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg
     6961 gacgggagtt tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag
     7021 tagcatccat taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt
     7081 tgttttgcgc tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt
     7141 ttgtcaggta gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt
     7201 gccagccacc aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt
     7261 ttttccattt catcgttttg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa
     7321 gtactttgga tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc
     7381 tacgattcgg ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg
     7441 gtcgatcaca cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt
     7501 tagttcaatc agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg
     7561 tccaatcgat agatattcag gctcagcttc atcttttgat tgcttacgag gttcaatccg
     7621 tctgaatatt gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata
     7681 gtcgtcagca cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag
     7741 catcaaaatc ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc
     7801 catcgttgga agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc
     7861 taatccttcg cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa
     7921 cgtcaagagc gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat
     7981 ctcctttaaa taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat
     8041 tttacaaaag aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca
     8101 gtcaatgatt ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa
     8161 aaaagaagta ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc
     8221 agaagtgatc ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg
     8281 attcgatgtg tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga
     8341 agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct
     8401 agtcatcatg aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt
     8461 ttccggaata aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat
     8521 gactgtgctc tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa
     8581 agaccaatcg aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa
     8641 tgaactacat ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat
     8701 acgaagtaag ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg
     8761 attatgaaaa aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa
     8821 atatgactgc atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac
     8881 agcaaattca agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag
     8941 cttaactgaa cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga
     9001 agctgacgta aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa
     9061 cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt
     9121 gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat
     9181 cacacttggt tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat
     9241 gatggctggt cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt
     9301 aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc
     9361 gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac
     9421 tgaagtcagc ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa
     9481 cgactatgta ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga
     9541 gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat
     9601 gactggtcaa gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg
     9661 ggtcgtcatc actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc
     9721 agtgacaggc gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt
     9781 attccaccca gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat
     9841 tgaaaaagcg cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg
     9901 ggaaaattca ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg
     9961 cccactggaa gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa
    10021 cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc
    10081 agcagaaaga gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg
    10141 ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa
    10201 aatgatgcag caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg
    10261 ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa
    10321 gaagaaaaag aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg
    10381 tctactgccg gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa
    10441 cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata
    10501 tcatgatact gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat
    10561 gcttgatatt aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt
    10621 cgatgaagca tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat
    10681 cagtgaacta atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt
    10741 ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct
    10801 ttggtcgttt tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc
    10861 aacaacaagt cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt
    10921 tatcggaagc acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct
    10981 tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat
    11041 gcgaaaaatc atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga
    11101 tgatcttcaa tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta
    11161 tgaaacaatc gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt
    11221 ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt
    11281 acaattgcca gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca
    11341 aatgacagaa ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt
    11401 ggaacatcaa atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg
    11461 gattccttta ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg
    11521 attcggtgaa tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag
    11581 gcaactatat gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt
    11641 actctgttaa tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta
    11701 atgttccttc aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg
    11761 cttccggttg ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt
    11821 ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat
    11881 cccaaacact gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg
    11941 gcacagaacg gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct
    12001 cacgaaaacg agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa
    12061 atggcagatc atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa
    12121 aagcttctgc tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa
    12181 ttttttttgt catacgattt ttcctccttc aaactatcta taataatctt acaaaagata
    12241 agtaactgga gcaaataaaa acacttactg acaaaagcta catagaagat agattactta
    12301 tttttaacaa aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa
    12361 gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat
    12421 aaaacaatgg aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc
    12481 taaaaagagt ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg
    12541 tttcatcgaa actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa
    12601 agaagactta gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa
    12661 catcctttca aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata
    12721 aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc
    12781 acttatcctg atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat
    12841 ctaacagtgg ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa
    12901 gcaatccgca caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat
    12961 atcatcgatg ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt
    13021 ttttgttttt tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat
    13081 ccttatagga ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc
    13141 tctattgtta agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag
    13201 cagtatatct gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac
    13261 tgaacaaaca atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga
    13321 acaacagcca aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat
    13381 cgcagcgaca catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct
    13441 cgggttgaat gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata
    13501 tgggtttgct tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa
    13561 tggtcagcaa gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc
    13621 aagttttctt gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga
    13681 aggccaagat cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct
    13741 accagcacct gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc
    13801 cgtagaagta agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat
    13861 gtctgcgttg aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag
    13921 cccatcattt aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat
    13981 aattttggct taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa
    14041 aacagacaaa aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg
    14101 taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag
    14161 aacgttacaa aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat
    14221 taacagtcaa aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc
    14281 cttctatcaa tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact
    14341 ctgtagatct agctgaaaat gaattctatt accatgagat catcggtctg catgtattag
    14401 aagatggaac tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt
    14461 gggtcgttca acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca
    14521 aagaagtcga tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg
    14581 atgaagattg atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc
    14641 atcatcggta aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat
    14701 tattcagata acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg
    14761 ctgctcaaag tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat
    14821 gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca
    14881 gaagaatttt cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat
    14941 gaacgtatcc gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg
    15001 ggagaattag gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta
    15061 gggaatcaga cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa
    15121 tatactcgcc cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat
    15181 cataaattga ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg
    15241 cctgatcttt tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc
    15301 aagaaagaag aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct
    15361 ttacagtagg ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta
    15421 cgatattccg ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg
    15481 aatccattaa tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc
    15541 cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag
    15601 ttatttgaag gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta
    15661 cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt
    15721 gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt
    15781 gcattacacg gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatc gttatcagac
    15841 agagaaagaa cccttggtat tcaagggttc tttttttatt atctgttttt ttatattaac
    15901 gatttctatt tggtacattt ttgattaata aaaaggggca aaaaaggggc aacaagttac
    15961 agtttttcta tatcttcttt caatttttct tttactcctt ttgtcacatg caaataaatt
    16021 ttttcagtga tatcactatt ttcgtgacca acgcgatcct gaatggcata cagaggagtt
    16081 cctagttccg ctaattttga aatatgggta tgtctaaaaa tatgtgaact aagtttttta
    16141 tcaatcttca tgtcagcttt atgacttctc aaataggtgt taattgctgt tagttggaaa
    16201 ggagttcctt tcgttgtttg aaatagaaat tgaccatttg gatttaattc tagaagctca
    16261 ttgtaaatag ctatcgcttt ttttggtaaa tcaatttctc gcattccggc agctgttttt
    16321 gtagaatcag attttttcat atcagctatt gaacgttctc tatacatcat cgtcccattt
    16381 ataactactg atgcattatt attggtaatg tgtacatcat ctttacttaa tgcgattgct
    16441 tcaccagacc tcatcccagt caaatatagc cactggaaaa ggagagaata tcttttattg
    16501 tgtgaagttg tgaaatctac tagtctatta tattcatcat cttctaaaaa tttatcttta
    16561 atttttatcg tttttgactc tcttttataa tcaataacta cttcatcgat aggatttttt
    16621 tctacataac cttttttcat agcatatgaa aaaagaagat tcaatttgga tttaattaca
    16681 ctgacgtact tatttgacaa gtcatcttta tatatcatgt cttcaaaaat attattcaaa
    16741 tctattgtat taataccaga aacgatgtaa gtctcaggta tcttcttctt tatagtgttt
    16801 aatatattat tcgtaggata atatgtagat tccttaactt gtcttttgta tataactaac
    16861 cattcttcaa caagctcatg aaatgttaga tcaggcttat gaagtgtttt ttcgtttaat
    16921 tttatatcaa tttttttatt taactctagc aatgccactt tctgagtttc tcgagattta
    16981 tttttataag taatgctcac tttttttctt tttctagttt ttggatcaat atatcgctca
    17041 ttatatttat atactttttt tccgttttta tcagttttag tttcgatcca cattgttttc
    17101 atctcctatt tgttgctata ataggaatag ataagtaagc cgattatagc aggtttattt
    17161 ttcatcacgt ccacaaactt tggcgaggga ggggcgtgtt tttgttagtt attgtggtag
    17221 gttatctaaa gcgtattgtg cttgctcatt tgtaaaacct tcaaatataa gttgatcgta
    17281 caattgcgaa tctgacattg atgcccaatc atcgtaactt tccgctttag ccaaagcttg
    17341 ttcgttgtaa tcgacatcaa cattagccag cgcataatcg attgcatcac ttggatattc
    17401 ttcaaactct agttgttcac gcaaaccttg ttcagacata ccagcccaac ctagatagct
    17461 ttcagctttg ctcaatgcat ttctgtattc acgaggaaca ctttcttctt gagtaacttc
    17521 ttctatagta gtttcagtag tttcagtttc ttcttcaacg actgtagatt ctgctaccga
    17581 ttctgcagaa gacacttctg tcacgctttc tttagttgtt tcagcgacgg tatttttagc
    17641 atcatcactg cctccttgcg aacctatagc aataaacacc acaacaaccg ctaacaacca
    17701 aaaccatacc cgtttataaa acggtttctt aactttatac attttcccat cttgacccat
    17761 aacttttttt gccatttaaa tatccctcgc ttcttgttat aatatatttg tgatctcaga
    17821 aatgaggtat gagtccgtgt tgcagcacgg gcttttttct ttataacttt tttagagatt
    17881 ataggcaaaa tagtagggca taaaaatgta ttattgaatt ccgtatttag aaaatcctaa
    17941 ttgaacttca ccggaagtct tttgctgtgt tgtacgcaat gcttcttctt cagacattcc
    18001 attctgtact ttccatgcaa caggcgacat cccgtatttg ttaacaaaat cagtaagtga
    18061 taaagtgtca gcgtcttgct gagcgcttgt ttgttggtct tctggattct gttgagatgc
    18121 tgcttgttgt tctttctgat cttgactgat aatattgcca gcatcatctg tagtcaatcc
    18181 attttcataa agggccacgc cgaaagcttc ccactctttg ttggaccaat ttgcacgatc
    18241 agctggagtt gactgtaaag tgcgttgttt catctgttca tatgtttctt cttgaggtgc
    18301 ggtttggatt gtactctgac tggagcttat aactgttggg ctaggttccg ccgtagcttg
    18361 gttggagctt gtaactgttg aactagaatc tgttttagat gtagatttgc tagtagatga
    18421 actggtttca gttgtttctt ttgttttact tatttttgtt tcttggttag aagtggtatc
    18481 tgttgattca gcttttttat tatttgaaca agctgaaagt agcagagcag tacttaacaa
    18541 caacataacg ctaacttttt tcattttata attcctcttt ctcgttgtaa tatgtgtgct
    18601 aacacgggct tttttatata agaaaacgat aagcgctttc tggaagcccg taaagattct
    18661 ttaattcctc gattttttta ggatattgat cattatcttc tttataaaga gaaacaatga
    18721 gattagcagc aaagcaatta gcttcgcttt cagatttgct tctagatgtt cttgttgata
    18781 cataatagct ggataagcca cgatgaaaaa tagcgtgacc taattcgtga gcgcaaatgt
    18841 agaatctttc ctcagagtct cgcagttcat catttaaaaa gattatcgca cgatctctaa
    18901 tttcttgaaa ctgccccttg ggattctcga taaaaggaac gtattgaatt ttaatgccca
    18961 tcttttcaca aatataaaaa ggattagcgg actggtattt ccgcttcaac tcctcgacta
    19021 aattaatcgt atccatctcc ataagctcac atctttttgc ctttttcttt gtcttctttc
    19081 acaatatccc agaaagtcgc tatcaggata tcttttacgc gctgtatttg ttcgggtgtc
    19141 aatgtttccc caccataaga catattaaca tttgagtcta gtagtttatc aagttcaacc
    19201 acttcctctt ttgtagccca cttgggaaca ttattatttc ccaataaata atcagttgtg
    19261 acaccaaaat aatcagcaac tttctttaag ttctcagatt ttggcgaggc tttatcccat
    19321 cttcttattt gtccattaga gatgcccacc tgtctttcta cttctgctat agtcacatgc
    19381 ttttcgtctg ctaattcttt aatcttagta actaaactca ttattatcaa cctttcaaag
    19441 ctgaaaagaa aataaatagc ttaaaagtta tatttttggt tgacaattag cttttaagct
    19501 agtatattta attcgtaagc taaattgtta gctaaataag agcaacaaaa aactctacta
    19561 atttaaaaca ttctctcggt cgccaaactt agaaatgtta ttttagaggc tttttataag
    19621 tcttatttaa ctatgtattc ataatagctt aaaagctaat aggtgtcaac gatttagcta
    19681 attttttagc ttacaaatta tttgtttaga aaggagctat ttttatgtct gagaatttag
    19741 acttaaaaat tcgagcggag atgagaaaaa gaagaatgac tttcaaagaa ctagctgcgc
    19801 ttatcggtat ttcaggagct tatttatcag atattctaaa cggcaatcgt gatggaaaga
    19861 aagcacaaca gcatatcgaa acagtgaaaa aaatattgga catccgatag ggggggtagg
    19921 cgatgacaaa actaaaaaaa caagattttg taaaaaaata taattattct ccatctactt
    19981 atcaacgtcg aatgtcggaa ctaaaaaata cagcaatttt ctcagcggcg tatgaacggg
    20041 tcacaggaca agaagtttgg atcaatacag aattatacga taaatttttg tctttcaaat
    20101 cctataacag gttacgcaca agaaaggtaa cacctaaaga atttatcgag aagcatttag
    20161 ttgatttata aaaaacagaa cattttgaga ggtgaaggtt aatgggtaaa ttcaacagag
    20221 cattagtatt cagcgcaccg ctaatcgtct atgctttagg gctttgggga agtagacaag
    20281 cgttgatagg aacgattgtg tacatggttt ggatttttat agggctggat gaagctgagt
    20341 acaaaacaaa aaagccagtc gggagggact gactaatgag taaggacgat attgtatttc
    20401 tgttaattat gttttgtggg attggatcaa taataattaa tttgtatttg attattaaag
    20461 aaaaattttt caattaaaat gagtctttta gctttaactt aaattttttt tgataattag
    20521 ctgttcttat gaccattaca agtttttcac caggtaatat atctttcata tctaaaactt
    20581 ctgaaaaagc gactgagtag ccaggagaga ttgcttgtgc caacggtata ggagataaaa
    20641 ggctactttt atgaatgctt cccatatcaa aaccattggc gtgataatgt gaacaattaa
    20701 ttttcttacc gttgtaaaga tacgtacatt cgataattga gtttggcatt tttgatgggt
    20761 ttgcaattac gccagtaaga atcattcgat tacttctatc atgcttaact aaaaaatagg
    20821 agagcgtaat ttttctttta tctttaaata agttatagag ggataaaaaa aatcctgaaa
    20881 tagaaatgat taaagtaata gcgacttggt aatcgctcaa aatttttatc aagtaattca
    20941 ccaccttttt actcaaatta tatcaaaaag gagagaagaa ataatgcaag aattagtaat
    21001 tttgaaaaat aaaaaaagac acgaacgccg gcaagcatga tcgtgtcagg gaaataaact
    21061 atctttctat attttaccac agaaaggaat gaaccgtaaa tgctaattgc aacggataca
    21121 ctagaccgca tctttttaaa agacgaatac aaactgcgca atatagatgc gtcaggaatt
    21181 ttagtatttg atctttatga caatggaaaa attggtatct atcaagcaag tgatattgaa
    21241 gaaacaaacc ttgctttcga acaaattgat gattctgtgg aattggattt agatgaggca
    21301 atcctagctt ttgaacaaat tgcaaaatta ttaaaggagg cacaaaagaa tggcaactct
    21361 ttaccaactc agcgagtcat atatcaaagt cctggaactg gcagaacaat tggatgaaga
    21421 aattcttcgc gatactctcg attcgattaa tgaagcgatc gagtataagg cagaaaactt
    21481 agcaaaaata gttaaagaag tagaagggaa agctgagtta atcgatagtg aaattaaacg
    21541 tctgcaggaa cgtaagacat cgcttttgaa caatgctaag agtatcaagc actatttaca
    21601 agaggaaatg gaaaagactg gtaaaacgaa gattaaaggt gaattattca acattggaat
    21661 tcaaaataat ccagtatcgg tcaatgtaat caatgagaac ttaattccaa aaggattctt
    21721 tacccctgtg cctcccaaat tggataaaaa gcaattgaag gaggaactga agcacggaga
    21781 tattcctggt gctgaactcg tccaaacaaa aggtttgagg ataagatgat gaaggaggag
    21841 aaataatgga acacgaaaac gtaaaagacg ctttgaaagc tgctatcgaa atcgcggaag
    21901 ctaaaggaat aaaggttgac ggcaaaccag caactgtcca tgacattcaa aatttgacga
    21961 aagaacatct ttattttatc gccgacttgc ttggtttgtc ggaattgtat ttagacaaat
    22021 aaccccacac ggaaggagtg gctagggtgg ttgtattaca agtaatagtc tgcgtaacat
    22081 tcatttggtc gtgcataccg gtttgtatat atatcatgaa gaagcttcgc cctgagtctt
    22141 acaaattata cgcaatttac agcataacag tagctcttgc atttacattg tttgcaattt
    22201 ctaggcttcc gttttaatcg tgggaatgaa tttttaatga actaaattat ctataaagca
    22261 aaaaaaggag aaacaacatt gaaaaacaaa ctagcaaaaa caacagcaat catcggacta
    22321 gcactaggta gcggcgttat cggctacgca gcaagcaatg cgtttcaaga tttggacacg
    22381 atcaaagcaa atttcaacac agtcctacaa tacggtcaaa cgaaatcgca acgtgtgtca
    22441 gaacttgaat cacagctatc caacaacact cgcacacagg agcagctgaa agccgaaatt
    22501 gagcaaatca aatcggacaa gcagaaggaa attgaagcca agcaacgaga agtcgaacaa
    22561 aagcaacagg agatcgctac aaagcaacag gaagccgata gcttgcgcca acaactatcc
    22621 acagtgcaaa acgacaagga gcagttagaa cagcgcgtga gcgagttacg gcagtatacg
    22681 gatcaaaagg taggggagtt ggggaaatga aagagttgac tatttatgac ccaaatattt
    22741 catgggctaa acacacaatt aaagtttctt ttatgatttg gggttacaaa ggatatgtta
    22801 cttacaaagt gggcggtaac actaagggtt tatcattaat agctattgat tcagatgatc
    22861 tatatgatgc taatttcgag gataacccag ttaatttccg tgaccttgat gaagattggt
    22921 tctctatgga acttactaac gataaaggcg acagtacact tgtagaagat gaattcgaca
    22981 gactaggtga ttatattgtc ggtgttgaaa ttattgcgca cgaaccagag taggagagtg
    23041 aatacaaaat gattaaaaga atcactatat cagttgaaga agatagtgga tcaacaatgc
    23101 agcataccga caatcgaaaa acttttttaa ccaatagaaa ggagaatttt gatgcagatt
    23161 aagaaggcga cggatatcga acaaaccaag ggaacatatt tgatctacgg tgctccagga
    23221 aaaggtaaaa cttcaacggc taaatatttt ccagggaaaa cactgatcct tgatattgat
    23281 cggacttcaa aagtccttag aggtgagaaa aatgtcgata tcgtatatat cgacaacgaa
    23341 gatacctgga acgactgggg caacacgttg gctgatttaa ctaccaatta tgtaggtgtt
    23401 tatgacaacg tggttgtcga taatgtcagc gaattggaac ggtgtattct gtccagttta
    23461 ggcgcagaag gaaaaaacaa cggcgttcct tcgcaaggcg attatcaata tatgcaattt
    23521 agaatggtga acagcctacg ctatatgaaa aatttagaca gcaaccttat ctggaccgca
    23581 tgggaagaaa tcgatttgtg gacggattcg gatggatctt cttacaacat cgctttgccg
    23641 cagatcaatc gaaagattcg aaacaatatt ttgggattat gtgacgttgt tggtcgattg
    23701 atggtaaagg aagacgatga acgcggattt attttacaag ccacagattc aacctatgcc
    23761 aaaaatcaat tggataatag atctggctgt aagcaaagtg agctgatttt agatggaatt
    23821 gtacgactac cagaatgatt tagtcgatcg tgcaagacaa gcctatgttg atggttacaa
    23881 ggcaccatgt atcgtatcac cgtgcggttc tggaaaatca gtcatggttg cagagatcgc
    23941 taaacgaacc acacgaaaag gcaacagagt gttgttcttg gtccatcgtc gtgaattgat
    24001 tgatcagatc aaagatacgt ttcaaaagat gggcgtagat aatcagcttg taaatttcgg
    24061 catggttcaa acagtcgtac gacatcttaa aaccatcaag aaaccacaac tgatcatcac
    24121 tgacgaaaac catcacgggt tagcagcgtc ctacagaaaa atctatgagt attattcgga
    24181 cgttccgcga ttaggtttta ccgcgacacc catccggtta aacggtagtg ggttggggga
    24241 tgtgaacgac ttgctaattg aaggagtatc ggctaaatgg ttgattgaga atcatagatt
    24301 agctccttat gaatattatg cacctaaatt gatcgataca gccgaattaa agaaagcttc
    24361 gactggagat tttacaaaga aatcaatgga taaggcagtg aagaatacta tttatggcga
    24421 cgtgttgaaa cattataaaa cgttagcaga aggtgaacag gcaatagcat actgtcacag
    24481 tatcgatgca agtaaacaca ctgctgaaat attcaacgat gctggttata aagcaacaca
    24541 tatcgatgca aaaactccaa aagatgaacg tgcagatatt atcgaagcgt tcagaaatca
    24601 tgaaattaaa atcctatgca atgttgattt gatcggagaa gggtttgatg ttcctgattg
    24661 ttcgactgta attatgttga gacctactca atcgttatca ttgtacattc agcaatcgat
    24721 gagggggatg cgttaccgac cagataaaac gtcaatcatc attgaccatg tgggtaatgt
    24781 gaaccgtttt ggattgcctg atatggatcg tgaatggagt ttagatacaa aaaagaaaac
    24841 aaaaactgat agtgatttat ctatcgttca atgcgcgttt tgttttggtg cttacgaacg
    24901 gccaaaaggc gataatattt gtccgtattg cggagagttg cagccgattg aagaacgaaa
    24961 gtcagagtta gaaatcgatg aatcggctga attaatgaaa gtcggagaaa cgaaaattac
    25021 tttgaatttt gagaacaata aatattacaa catgacggaa gatgaagcag aaagtataga
    25081 ggacttatat gccatagcaa gagctaaagg atttaaacct ggttgggctt atatggctgc
    25141 taagaggaag gggtggttgt aggaatgggc ggtatttttg gtaaaaaagc agaagatgtt
    25201 caaggttcaa taataaatgg cgtaacaata ataggtgata ctggaaaaag agatagttca
    25261 ggcaatcaaa ttgtaatcgc tagaaataaa aataacgaag tttttgaatc ttctctatct
    25321 aatataaaaa gaggtgttgt taacgggaca ggcgaagaga gtggctacgt taatttaaaa
    25381 aaagctaatg aaaaatacca tgttgaagga acttacgtta aggctttgtc tgtcggtgga
    25441 acaaaaggcg ttaattggga taaacgaaga aataaatgga aagcacgatt atatttcaaa
    25501 ggagaatatg tcttacaaaa ggatttcaac accaaagaag aagcaatcca agcacgactt
    25561 gcagcagaag aaaaatattt caaacctatc ttagaaaaat atgaaaataa aaaggagaat
    25621 gattaacatg acaggattta acttagactt ttcaaaagcg caacaaggga acgaaatcaa
    25681 agacggtacg tacgaagtgg tcgtaaacaa agctgtagaa aatgcaacta aatcaggcgc
    25741 agaatttatc gatatcgatt tgatcgttcg taacgatgtg gatcaaccgt tccaaaataa
    25801 acacattttc gcaaaaatct ggaaagccaa agctaccggc aaatataacg aaggtatgat
    25861 catggcaatt gcacaagcgc tacagttgga agacggaaaa tcatataacg ggtttgatga
    25921 attgctggcg gactttgttt tgaaaaccgc ctcagtacgc gtgaaaactg aagagtcaaa
    25981 tggatataaa aacgtcaatg tgaagtcttg ggataaaacg aacacacggg gcgtaatgaa
    26041 tcaccaattc aaaaatggtg atgaaccatc cttcggaccg gaacgttcaa gagcgacaac
    26101 ggttagaaat gacaatcttc cattttaatt gaggtgtaat caatgtatga aaaaattcca
    26161 aaagagctta aaaatctcaa acaatggtgt gtttataagc tagtttggga tgaaaagcgg
    26221 cagaagcaca cgaagatccc ttataacgcc aacaacgggc ataaagccaa atccaatgac
    26281 gagagtacct ggtctgattt tcagaccgct ctcgctgcta ttgatcacta cggaatgagt
    26341 ggactggggt tctttttcaa accaccttat ttcggcattg atatcgataa tgctgaaggt
    26401 gaagttgagc gatttaaatc aggtgatata gaagaaaata ttatctatga atttatcgaa
    26461 agcatgagat cctatgctga gtattctcaa tcaggtactg gcatccacat tattgctcgc
    26521 ggagaattgc ccggcggaag acgcaggaaa ggtgacgtag aaatgtacca gaacggccga
    26581 ttcttcgtta tgactggaaa tgccgcatca aaatatttag aaataactga gccgaatcca
    26641 aaggatatca aacgcctata tgatcgctac gttggagatg aaaaaatcat ccaattcaaa
    26701 gaagaaaatc cgctgatgaa taccgttgat ttaccggttg aagaaattat caaacgtgct
    26761 gaatcgtctt cccaaggggc aagattcaaa gtctttatga acggcggttg ggaagctttt
    26821 tattcctcgc aatccgaagc ggatatggcg ttcgccaacg acttggcttt ttggaccggt
    26881 agagactttg aaaaaatgga tgagattttc agagcgtctg caatgatccg tagcaaatat
    26941 gaccaaaaaa ggggatcggc aacttacgga gaatctctac taaataaggc aattgctgat
    27001 acaaattccg tctacaaccc caaacggaaa tctgatttta agatttttat caaagaccag
    27061 gaacagccaa aagaggaaaa atactattcg tacgacgaca ccggcaacgc ggatcggttt
    27121 actgatatct acggaacgtt agtcaaatat tcgtacattg ataaatcttg gtattactac
    27181 gatgggaaag tttggctaca ggacaatacc ggcgaagttc ggaaaatgat cgatacgaca
    27241 gtagacatca tgggaaaaga accgttaacg atccctgaag gcgcagacga tgaaaccaaa
    27301 gaagctttga tgaaagcgaa agaaaaacac gtcaagcgtt ctcgcagcaa tgccggaaaa
    27361 aacgctatga tggacgaatt gaaacatcga ctatccgtat tgccggaaga gttcgacaaa
    27421 gataaaacgc tgttcaacac acaaagcggc tatctgtctt tgcatgacgg tcttctacac
    27481 gagcatgaga tagacaaaat gtttacacgt gtttcaaatg tcgagtacac agaatcggtc
    27541 gattgtccta tgtgggaaga atttattaat cagatttttg acaatgatca agaattaatc
    27601 agatatatcc agaaatgtgt cggatattcc ctgaccggat ctaccagaga gcaatgtatg
    27661 ttcatcctct acggtcatgg gtcaaatgga aagtcggtat ttttggaaat tatttctgaa
    27721 ttgatgggaa actatgcgat gactatgcag gcacaaacga tcatggtcaa gcaaagccaa
    27781 tcatcagcaa acagtgatat tgcacgattg aaaggtgcgc ggttggtcac ttcgtcagaa
    27841 ccgaatgaag gcgtccgact ggacgaaggt ctggtcaaac aattgactgg tggtgataaa
    27901 gtcacggctc ggcaccttta tggaaaagaa ttcgagttcg aacctgaatt caagttgtgg
    27961 ctggcaacga accacaagcc catcatccgt gggaccgatg acggcatctg gcgacggtta
    28021 aatttaattc cattcactgt ccaaatcccg gatcacaaaa aggataaaaa tctgaaattt
    28081 aaattgcaga cggagttgca agggatcctt aaatgggcaa tcgatggttg cctgttgtgg
    28141 cagagagagg gcttggagaa accaaagtcg gttgttgcag cgagtcagga ttatcgaaac
    28201 gaaatggatc aaataggtac atttatcgaa acgtgttgcg aaacaggtcc aggactaaaa
    28261 atatcaggcg gagaattata caaagtttat cgtgaatggg catcagataa tggcgagcat
    28321 actttcacta acacgaagtt tggcagagag atttcaaaga aatatagcaa agaaaaagct
    28381 ggtggattta tggtttataa aggaatcaca ttaaaaccaa gaaaatacga taacgttaga
    28441 gaacttttta aataaatctg gagggttctg gagggttgag aacgaaaccc tccaaccctt
    28501 agaaacgttg atatatcagt atttatatta tatttttctt tctttctgga gggttttaaa
    28561 aaaataataa agtattaaag taaaaaataa aaagtaaatt atataaaaaa gaataggttt
    28621 ttgggtctga ccctccaaac tctccaccaa aatcttccta aatgctttga taccaacgat
    28681 taaacagtct ggagggttac attgaaatca gaacatcaaa tacaagacga gatcagaatt
    28741 gccttatcaa aacatggttg cacgattttt cgatccaatg cgggacaagt gacaacgatc
    28801 gaagggcggc aattttacgg gatgccaaaa gggttcccag atctatgtgg acatcgtaat
    28861 tctgatggga aagcaattta tatagaagta aaaaatgaac gcggtaagtt gcgtgaagat
    28921 caaaaaaggt ttgctgaatt tttaaacggt caacctgtac tatacggtgt tgcacgatca
    28981 gtggaagaag cattgaaaat tgtggaggaa tcatcatgac aacagaagaa gtgattcaaa
    29041 tgcgtattcg aagccttcag cgtgagattg acgatctgga acggacaaag gcagtgatgg
    29101 tcaatgaaac ggcgaggaag gcaatcgatt tgcacataga gaatttaaga agggaaatcc
    29161 atcgattgga ggaatgagcg tggataagaa agcagcaatg aaacgaatca tcgaactgac
    29221 acattctgag aattggcaag aagacaaaga aatagttgca gaagtccaaa agctcggtaa
    29281 atcaatgtgg agtgaaaaaa ccaaacggag aacaccgaga aaaattgcaa tctggcatgg
    29341 tgaccgaatt ctagtaacag gtactgctga acagttatct gaaattactg gattaagcaa
    29401 aaacattatc tgggatagag ctaaacgcga gaacgttgat tcgaaaggtc gtcaatttaa
    29461 acattgggag aagaaataat ggaggaacta atcacaaaag tagagcagtg ggcaaaagat
    29521 aagggattgg atcaagctga ttccagcaag caaatgttaa aaacgatcga agagattggg
    29581 gaagttgccg cttctctagc tagaaaagat gaatatggtt taagagacgg aattggagac
    29641 gtagtagtaa ccttgattat tttagctatg caaaatgata tggatttgta cgagtgtctg
    29701 aaccaagcat acaacgaaat caaaggacgc acaggaaaaa tggtagatgg tgtattcgtg
    29761 aagtcgagtg atttggagga cagcgatgaa taaaaaagta ttaattgata aacaagcatt
    29821 gattgatgaa ttaatgaaaa tacctggtgt gggatctaat agtgacgctt tagaaacgat
    29881 taaacgtttc ccatcgtatg aaccgcagaa gccagttgtg cctaagtttg tggcggaact
    29941 actcgactat tatcgtcagt caacagatgt tgatttatta gctttattga taactttcca
    30001 tgattggtat tatcgcaaaa ctaaagacgg tgagcatgaa gaagcaattg attggctagt
    30061 tgatcatcct gaaatttata tgcgtgcatg gctgtttggc tacgagctcg agaaagagcc
    30121 gttgtattgc gtaataatca atggtagata tcttgtcaaa gtattcagca atacaaacgt
    30181 tgtcatatta gttccagcag atgaattttc acagtacgtt actcaaaaat ttcaattaac
    30241 tgaaaaacaa atcaaagcaa ttgatgaaag atactggccg tttgctgtgc cagtggatga
    30301 ggtggtagaa ggatgagcga cccatacatt actaaattag aaaagttgtc cgaagatttt
    30361 gagaagaata aaaataaact aactaaagat aaacttatag aactggttag gttggttatc
    30421 agaggagcta ttacaagaga aaaggcgcag caaaacatag aaatttttgc tcgtagtgtc
    30481 cgagatggat tagagaagaa agttaaacaa aatgaacaac agacaccgca gaataacaaa
    30541 actaagaaaa cgggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc
    30601 agcagaagaa acatataaag tggcaagtca gtgtgttgct gatgcgagcg gcgctattcg
    30661 taagtttgga atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta
    30721 aaagacggat tttactccag cagtcatggt atcggcggtt taatgctaga tatgccgaca
    30781 aagaacccta aaacacgtga gaaaccaaaa ttcaaagtcg gtgacatggt tcgctgtgaa
    30841 gcagaaggat tcatctatcc attttgtgga tatgtagaac atctctataa tcactcagca
    30901 atcattcgta ttgaaaacac gatggaatgt gacaagtggt tagcgaaaag caaagagaat
    30961 ttagcagtag cgagattggt ggatatggag gttatataaa cagcgaaaaa atgtacttct
    31021 aagagaaaaa tttatttttt ataaaaatag gaaagtatca ttatgaaaaa gaccacagag
    31081 ataaataaac ctaccacata aaaaatgaat atttcttgcg attcaatata ggtatattta
    31141 acaatttgta caataaaaaa tactataggt aaaaaagata acatgaggag cttatttgtt
    31201 ctagagtgtt tgacaaagta acaaaatgcg acgataccaa tacaaacggg aatccaaaat
    31261 aacagcatgc tccacatagt cactcaacca tctcctatac gctttattat caatagataa
    31321 tatcattttt cgtaataaag ataaaaaaat aagtaaggct tctcattaaa atataaaaaa
    31381 gacagccgac cactgactgc ctatataaga gtattgaaat aaaaagctgc tgatataata
    31441 aattccacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
    31501 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
    31561 ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat tacggatatg
    31621 ccgaaggcgc cgaagcacgg caataaggca gaagacgcaa tcattcagat gatggatata
    31681 gaagcggaga gagacgcgat tctagcagcc ttgatggctc ttagtctgat tagtcgtcag
    31741 atactctact atagcttttg tgtgccagat agcttctcaa actacagaat tagccgtgaa
    31801 gtgggttatt cagaaagaag tatacaacgg atgaagtcgg aagctctaat agagtttgca
    31861 gaagcatata aacacggaag aataattgct tataaataat ttggcggctt tttggcggaa
    31921 tgatggcggt ttttagctat ttaccagtga tattatggta gtgtcgaaag attagtgata
    31981 ggtctaagac aaaataataa taaaaggaac atcgttttat tattgtttca caattaagct
    32041 tcgatagaca gcaacggaaa tattaagaat aaggatgtga attttaactc cttctaaatt
    32101 gttcttatta tctatcatcc gttgctgtct attgtttttt taattattca cacgataact
    32161 aaaggtgagc gaagagaaat gattccatta ataatttcaa tttttgcgct ttgtcttaat
    32221 gtctatatga ttggatttaa aaatgggcaa aataaaagat agtagtaact aagaaagaaa
    32281 tttttatatt gtcactgtgg cggaaagggt agacgctaag catgtgtgct aggtcaatgc
    32341 ttcggcaact atgcaatgtt cgattcattg ccagtgactt tggcaaccga ggcatcggcg
    32401 gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg tgcatcgtgt aggttgctat
    32461 tacatattag atcactcgtt gagtggtctt tttattttac ataaaggagg taacaacgat
    32521 gtatagacca caatacttag aacagaagta tgaagtaatc actgtgcaaa acggtaacgg
    32581 tgagatagta cgaaagtata gaagaccaat aaagagcgat acatataaac gaaaggaaaa
    32641 caatgaagtt atttcttttc gcagaaggag aaaagccaaa tgagaaacta ctggtatgta
    32701 tcgctaacta atgaatatcc tcgaaccatt gatgattgtt cagtgcgtgt tgtgcgttct
    32761 gtacaaatca aagggaagta ctctattgtc gaaatgctaa gagaagctac accaaacgaa
    32821 gtggataaat gcaagctgat atattgcggt catggctatt ggaaagacga gtatatccaa
    32881 caaaacattg agaggtggat agatagatga gttacctcga acatttgaaa cgttgctaca
    32941 tgcattctaa gaataaactt cctgacagct acactgtaga tgatgttgct atacatgtat
    33001 tgaagactga gagccatagc agtcctgatg gtagcagtaa ggagcagacg ctcgcgtggt
    33061 tcaagttctt taagtggatt aaggaggaag agtaatgcac atgcacatag gagaaaagat
    33121 atataaattg agacggctta ataacgaaac aatgaacgac ttagttaaac aaactggagt
    33181 gtcaaaatca tggttatctg atattgagtc tggaaaaaag caacgcgttg attttaataa
    33241 gatatgtgca atagccaaac attataaagt tagcttagaa tactttcggg atgattattt
    33301 ggatagtgat gaacacgagg atattattcc tttgacaggc gaaacaagag ttaaatcagt
    33361 gaaatcgctt gaacaaagcc taaagtatct gaataacaaa caagaaccta accttctaga
    33421 gatcaaacta aaagatactg actcagtacc agaggtttgg tacaagggtg agaggttgga
    33481 tgaattgcct aaaggattag tagatgtctc gtatcattgg aagactgatg attttactaa
    33541 tgatgataga ggagcgaacg acatcacgat tcaatacttt tctagcttta atgataagta
    33601 tccagataag aaaacaatcg gacacaagag agatatgtaa atgaaagaag ttagacctag
    33661 agacgaaata gacaaactat acaagaccaa acgatggcga gacctaaggc aagtagtaat
    33721 agctagggac ttcggcatgt gccaagagtg caagcgtcga gggcggaaca caaggggcac
    33781 gatcatccat cacatagtcg aggcgaggga agacctgtca ctgttctggt ccgtagataa
    33841 ccttgaatgt atctgtgtag cttgtcacaa cagagagcat ccagagagat caggcgggaa
    33901 gaagaaacca aaacctaaat cacatatcgt taaaatgtat tcaactcctg aaagataagt
    33961 ttgcagcgaa atgaaggtag cccccctact ctaaaagatt aaagagtaag gcttgagaag
    34021 aacggtgctg tccttccttc gtaaaaagac cgcttttcaa gttttttgga gaaaaaggaa
    34081 aaagccgacc aatttaagcc ggctttggac gaagctattt cttagtccat ttgtttcctt
    34141 tttgagaagt agggggtaat cggtcgcctg gatcaatttt tacttcttgt ccgccttgga
    34201 catttccacc acgaggaccc acttctttgt aggttccttt tggtttgttg tcttcgccgg
    34261 gtttatagag ttctcccata ggaatccctc cttaacaaat ttcggcacag cactgccgat
    34321 aacctaatta taaggattgt gataacgatt tcaatctatc ttttgaaaga aggtgatatt
    34381 atgccgcaac cagcgaagag tgcaaaatta caattattaa acggaaaccc aaataagaag
    34441 aataccgaag aactccgcaa gcgagcggcc gcagaagaca aattaaaaat ggctactgac
    34501 aaaatcaaac cgccgttatg gctagattcg ctaggaaagg atacctttga gtttatcgcc
    34561 gatgaattgc tgtctgtgga tttaatcagt aatccggacg tccatacaat ggctctatac
    34621 tccaattggt attcgcaata cgtttcttta gaaaaacagc ttcgaaaact acaacgagag
    34681 tacaagttga actatgcgct tgcgaaaaag gaggcagagg cgagaggtga gccgtttaat
    34741 gaacctaatg aattaattgg taatccgctc tctcggcaga tggatacagc gtctcggaat
    34801 ctccgttctt ttggcgctga tttaggacta tcaccagcag ccagagctaa gttagctatt
    34861 aagatggctg atgatggtgg tgatgacgat gacgacttct aatattttgg atatgtctta
    34921 cacagaacgt gtggactatt ggcaaagcta tcttgaggag caagcttctt ggggtggctt
    34981 tttaaaatgt ccatatccgg aattgttaac tacttggtat gcggaacgat taatcgatgg
    35041 aagcatacca gccagcaaag aaaatattca agctgctaaa cggcatatgc gtgatttgca
    35101 gcgccaagga acagatgatt ttccttggat ctttgacgaa gaaaaaggtc accggcctat
    35161 tagatatatc gaaaaaaaat gtaaaccaac tgaaggcgac tttggttcgt ttgttttgca
    35221 accttggcag catttcataa ttggatccat gtacggatgg gtacatcgtg atacaggaga
    35281 gcgtcgcttc cgcgaggctc ttatttttgt tggacgtaaa aacggtaagt tttgccgttt
    35341 aaaaatcggg caaaatcggt gaaaaccttt atttttggct ttcttttagt aataattcga
    35401 tagctttatc aagcaattta gacataggaa ccatagtttc ttccgacatt ttttttaatc
    35461 cttcgtataa atctttatca attgcgttag aaattctaat tctgtttttt agtccgtatt
    35521 tgtccatgtg tgacactcct ttttatttta ctataacatc cgattccacc gcttgcaact
    35581 accatcaatt gatggtagta tataaaagag gtgagtagat tgaaaaaata cattatttac
    35641 aaacacttaa ataaaataaa tggcaaaatt tatatcggtg tcacaaatga tattggcaga
    35701 aggtggagga gcggtgggat tgaatataaa ccaccaaaga acgaaaccca acaccacaga
    35761 agtttttgga atgcaataca aaaatacggg tgggaaaatt ttgatcattt gattattgaa
    35821 gaagacttaa cgatgaaaga agcttttgag aaagaaaagt tttatattga actttatgac
    35881 tcaactaata agaaaaaagg ttataacatc gcaaaaggcg gaaatggcgg aataatatat
    35941 aaagttcacc ccaaaggaat gctagggaaa aaacaatcta aagaattttc ttcaaatcac
    36001 agtaaatggg caaagaacca taagaataat tgtatgacaa acggtgatgt tgtatggggt
    36061 gttactcacg aacacccaaa aggcatgttg ggaaagcacc acagcaaaga atcaattatg
    36121 aaaaagaaag catatagtgg tgaaaatgct gtaacctcaa aaccaattgt cgcaattgaa
    36181 cgggatggaa ctaaaagaga atttcacagc gctaagcttt gcatggctta ctattcgatt
    36241 agtacatgtg ttttttaccg acttttaaaa gatggcgccc cttatgtgat aaatcctaaa
    36301 gcgaactaca gaaataaaga aaaaatatta gctatcgaag gaattatgtt caaacataaa
    36361 gaagataccg aggtaagtta gtagattgcg aaaggttact aactaccgta acgcgtagga
    36421 gttgaataaa tataatgctc ccaagagtgt ccgacagcag ataatttgct gaaaatgtac
    36481 gctaaactgg gccagaactg actgaccgat gaaaatgagg gtgacctcca gagtgcgaga
    36541 taaaaagctc gcagataata acaatcgaaa acatcactaa tttcaggcct ttccacatac
    36601 atggtcgctt atgatgatga acaaggcgcc aacgtttacg tattggcaaa tgctcgtgat
    36661 caagcaagct tgttgtttga taaggccgca gaaatggtca aacaatcgcc ggcgctcttt
    36721 aagaaatttg gtaagcctaa acgatcaagt attaattatg ctcccgcctt ttctaaaatg
    36781 gaaccacgcg cctcagatag ccggaaattg gatgggctaa acactcactt tggtattttt
    36841 gacgagatcc acgagtttac gaattacaat ctgatcaacg ttatcaagaa atcaagagga
    36901 accagaaaac agcctctgat agtttatatc acaactgctg gatatgtatt agatggtccg
    36961 ttgatgtctt attttgagca aggtgtggac tgtttggaac atttggaaga tgacatcgat
    37021 gaacggactt tctattatct ggcaaaactt gacagtgcgg aagaggctga tgacccaaga
    37081 ttatggatca aagccaatcc gaatatttgt ctaatgaatt ttgttggcat gctagatgac
    37141 tatgttaagg ataaaaaaga tccaaaagaa tatgctgact ggattaccaa gcaatttaac
    37201 ttgttttccg atatcgatga gctgtcattt gtcgatatgc ctaccattaa acgaaacaat
    37261 aaaaccatcg atattgaaac gctcaaaggt aagaagtgtg tcggtggttt tgacttgtcc
    37321 gaaacggaag actttaccgc agccgtttta gaatttccgc ttgaaacagg cgaggtattc
    37381 attttgcaac acacatggat cccacaagct agatttgatc gagataacaa tcaagagcgt
    37441 atcaaagcgt gggagaaggt gggagatcta acgattattc ctggtgatta cgtcaattat
    37501 gaatacgtct taaattggtt tgtagaaaat tcgaaaatct atgacattgt aaaaatcaat
    37561 tatgacaagg ccaaggcgct acgattaaat aaagaactag aaaatgcagg atttgaaacc
    37621 aacgagattc ggcaagggtt tctatcatta ggtgggccaa tgcaaaactt caaggaaatg
    37681 ctattggacg gtaaggtgat tttcaacaat tccaagcttt accgatggta tctatccaac
    37741 gtcaagctgg tgatggatcg caactcaaac tggatgccgt ctaagcagtc caagagtaga
    37801 aaaatagatg gttttgcagc aagtttgaac agccacgccg aagtgttgaa tatgttggtt
    37861 aatcctgtcg gaaccgggaa agtaacctat tactcgattt ccgatttaat gaatatgtaa
    37921 gaaaggtgtg gaggaatgag tattttagat cgtttgcgtt cttttggccg agcgaagccg
    37981 aaagcgagca aacaagagta ttttttgaat gacccgggat tgataccgta tttagtcgga
    38041 aaagatgaaa tatcagaagg gattttttcc gtaattagcc gtgtatcgaa cgtttttgcg
    38101 tctctccctc tcaaaatgat agatgtggag tttggccaac cggacgactg tcctgcatac
    38161 aacttgttga gcgaaggccc tcgatatttt acaaagtttg attttttccg ggacgtggaa
    38221 gttttgagaa actaccaagg gaatgcgtat gtgcagattt tccgaaatat caatggagaa
    38281 gtagcagata tggcgttagt aaaacctggt gcttgccatc cagtgattga tatggatagc
    38341 ggggagcttt actaccaagt aactgcgact gacaaaggca gttacaagca agttatctat
    38401 gtacattaca tggaaatgct tcactttaaa caaccgaggt ttggcggctt ggaaggtaca
    38461 gaccccacaa aagtattaac gaataccctc ggatatgatc gagaagtccg aaaaatctct
    38521 ttaagtcagc ttaaaggaag taatgaaggg ctaaaagtta agtttgctag caatatggat
    38581 gaagaagcta aaaaagctac agttaaaaac attgctgatt tttatcgaca aaacggtgga
    38641 ctacttgtgg aagaaaacgg tgtagaaatc gaacgtttac aacgagagct ggtagacagc
    38701 aagcttttag atactgataa agtatctcgc tccagaatcg ctatggtcta caatgtgccg
    38761 gagcatttca tcgggaataa ccagtcgagt tactcctctc aggaacagct caatatggaa
    38821 tttttgacgt acaatctagt accgaccgtt aatcaatatg aagcggaact aaataagaaa
    38881 acactatcga gaaccgaaaa agcaaaaggt tatcgatata agtttaatgt cgcaagtttg
    38941 ctaagagctg acactcaggc aaggggacaa ttctatcaaa tcatgcgacg aggcggagca
    39001 tactcagcga atgatgtccg aagatttgag gacttgcaac cgataaataa aaccggtatg
    39061 gatgattacc atatttccgg agacctatat ccaatcgata tggatccaac attaagaaaa
    39121 acaacctcgt ctaaaagcgt agccgaaaac ggttaggttt ttttagtttg caccgaaggg
    39181 aggtggaaaa atgaaaaaag tgacgttaag cggcgatgtc gtggataacg ataccgcgtg
    39241 gctttatgac tggtttggga tcaattgtat ctcaccaggg aaaatttctg ccgctcttac
    39301 agaagcagcg ggggatgaag tagaacttga tatctcatcg aacggtgggg atgtcctagc
    39361 ggcaagcgaa atatataccg ctatccgtgc ctatccggga aaggtatccg gaaatgttgt
    39421 gagtattgca gcaagcgctg caagtgtaat cgcttgcgct tgcgaaccgc ttagaatctc
    39481 acctacggca cacatcatga ttcataacgc atgggtgacc actagtggca acgctgagga
    39541 attaaaagcc aatgcagaaa tgttaagcag tgtggatgag tctattgtta atgcttacga
    39601 gatcaaaaca ggactcgatc ggaaaaaact tgctgattta atggcgaaag atacttggtt
    39661 aaatgctcaa acagcagtag cagaagggtt tgcagatgaa attatgtttg cagaagcacc
    39721 agtaacggta ctcaatgcct ctcaaccggt tattccaaaa aacgcagtaa ctaagttgaa
    39781 aaatttaata ctcaaagcgg aagcaccgca agaagaaaca ctcttacaga aaaaactaaa
    39841 agccttaaat ggagggaaaa acgaatgaat ttagaacaat taaaaaatgc gtgggtcgag
    39901 gcgggaagta aagtctctga cttaaatgca caactcaatg cagcattggt tgacgatgaa
    39961 aaaacagaag aagatgtagt aagtttgcaa gcacaagtaa aagcagcacg ggctaaacgg
    40021 gacggattga aagagcaagt ggcaaatatg gaagccgaac aagtcttaaa cgtcaaaaaa
    40081 gaaccattag ataaaaaaga tgaaaacttg aaaaacaagt ttatcaaaga ctttaaagcg
    40141 atggtcaatg gtgatcctgc tattatggct actttgacat ctaatacgga tgaatctggt
    40201 aatgctatcg gattgactat tcctgtagat gtgcaaacga ctattcatac tttggttcgt
    40261 cggtttgact ctttgcaaga atacgtaaac gttgaaaaag tgaccactac cagcggttct
    40321 cgcgtttatg aaaaatggtc tgatattaca ccgttgactg ctttggatac tgaagacggt
    40381 gaaatcccag caaatgatga tcctgcactt tacttgatca aatacttgat caaacgctat
    40441 gcaggtattt ccacagtaac taacagcttg ctaaaagata ctgccgaaaa cattttggca
    40501 tggttgtcta aatggatcgc gaaaaaagta gttgttactc gcaatacaaa aatcttggca
    40561 gctattgatg gaatcaaagc ggcacaaaag aaagatgtta cagatgttga tggaattaaa
    40621 gatatcgtaa acgtccaact tgatccagct atcgaagcta catctatgtt tattacaaac
    40681 caagatggct tcaatgtttt agataaagtg aaacgtgctg atggatctta cttgttacaa
    40741 aaagacgtag cttctgcaac tggatatacc ttcttaggta aaccaatcaa gaaaattgct
    40801 tctcgtttct tgccaaacaa aggtacgcaa gctactccta aatatccact gtacattggt
    40861 gatctgaaag aagccgttac attgtatgat cgcgaaaaca tgagcttgct gacaacgaat
    40921 attggtggtg gagcttttga aacagacacc actaaagtac gcgtcatcga tcgcttcgat
    40981 gtgcaactag ttgatgatga agcggttgtt ttggctactt ttacaactat tgcgaacgag
    41041 acaccggcga aagtttaagg agctgattcc ttatgattct tgatcctaaa atggatttag
    41101 gcgaaatcaa aaacgcacta aagattgata ccgatgatga cgatgtggaa gtaagccgtg
    41161 cggcacaagc tgcaattgca tacattagag gggctatcgg gaatgataag ccctcttttt
    41221 ataagcaaga aaatgaaacg gttgatctga ttaatttagc tatcctgcaa ttagcagatc
    41281 attattacaa cgctcgatca gcaaccgtga gtgggaactt gcgagagtac gatttaggtt
    41341 ttacaagcct aatcttgcaa ctcaaagcaa gttatttgct ttttgtggag gaggagtagc
    41401 gtatgcccct tatccaaaca ggaaatttaa atcaacgcat caagtttgtc cgagatacga
    41461 cggttaagga tgaggacggg caagttgtcc cgacttctac aaccattctt acttgctggg
    41521 caagtgtgca gacacaacgc ctgaacgata ttaagacgtc gattggtacg gctttggaag
    41581 gaacactgac gttcattatc cgctaccaac aaaaatcaga gctgaccaat gatatgaaag
    41641 tgcgttggaa tggaaaaacg tttgaaatca ttacgattac gaaaggcgag tttgcgaagg
    41701 acttcacgac aatcattgca aaagaggttt caaaatgagt gtagaagtcg atgcaaccga
    41761 agtgtacaaa gcgcttaggg aagtaaaagc aaacgttcaa cgagtggaaa gcccagcact
    41821 tagaaaagct ggggagtacg ctcaagaaga gttacgacaa aacacacctt actgggatgg
    41881 aacgaagtca aacggtaaac gtggttcgta tatgcaagaa catgctaaga accatgtggt
    41941 tacaagctcg ataaaaaacg gattggtaga agtcggctat gacaaagatg tttcttggcg
    42001 gatgcacttt atcgagttcg gaacaatcaa acaacgtcca aaaggtttcg tacaaaaaac
    42061 acaaaagcaa atcgaaaaac aagtaacaca aatcattgct gacgaagtaa aaaggaggct
    42121 aggactttga aaacggcagt atcacaagtc tattcaattc tgaatagcaa tgaaaaaaca
    42181 aagaacattg atttttacac caatagtgtt ccggaatcag ctcaaacagt acctagcctt
    42241 ccagttggca gaattacaga gatatccggc aactatgaag attttgcaag caacaatcct
    42301 ttgaccattc aatttaacgt acaggtagat gtatgggtgt caaccatgaa agaggttgat
    42361 gccttttatt ttgcccttga tgaggttatg agggggaatg gttggcaatg cgcatacacg
    42421 gaacaaacag atgacgagga cttggaaggt gcaaagcgga ttatcaaacg atatgtagca
    42481 aatatttcac taaattaaaa ggagagaaaa tagatggcaa cagtaggatt cgagagcgtc
    42541 atttttggcg taaaaacagg tgcaggcggc actctaaaag aattagtagc agataagtcg
    42601 aaaggcggag cgatcgaagc taaaattact ggattaggcg caacttctaa cacaacatac
    42661 gcttcaaacg taccgttctt cattgcaagt aaaggggttt cgtcgccaaa agttacgctt
    42721 gacgtggcag acttaatgga taacggcatt tacagcgaaa tcattggtgc taaaaccgtg
    42781 gatggtgcaa atgtaattgg ttcagaaact gaagcgcctt acgtgtcggt agtcatggtt
    42841 acagcgaaca aagaaggaaa acgcttgttc atgggattga caaaaggaaa attcagtcat
    42901 ccagatatcg acatgaaaac agctgaagac aaaggggtag aattgcaaac cgattccatc
    42961 gaaggggaat tcatttctga tgaacgtggc tatgtatact taacagccgt agaatcagaa
    43021 ggaatgacct tacaaaaatt caaggacttg gtaaataaca aagaggggga gtagttaacc
    43081 ctgcatctac accaacgaca gatacaggga caccaaaaga accagaacca aaaattgata
    43141 cacaaggtta gccatttttg gctagcctta ttttttgtaa aaacaaggag gaaaacaaat
    43201 gattgaattg caattgaaac ttgacggaaa gaaaaaaaca ttcaaacaac aagatatttc
    43261 cgcacgtgca atgcgtgagt gtatcaaatt ttacgagaaa gcggaaaaag cagacctaac
    43321 tgatttagaa gcgattgatt caatgattgc aattacagca gatattttcc aagatccagc
    43381 agttacattt gatgctattt tagacggttt gactgcgagc gagttagtac cggcattaga
    43441 aagtgttttt gaacaaataa atgaactggg aaacaatgaa aaaaagcaga cggcgagcaa
    43501 aaagaaataa gtttttctga agctaggaaa gcaatggatc aaatctacaa agatttaatc
    43561 gaagcaggtt ggacgatgag agatgtggac gaagccgact atcattattt gttacacctt
    43621 tttggagaag tggagagtgg cgaagaatat gtagatggtg ctgatttcat caaacaattt
    43681 ttatcggctg aagacttagt aaaacttgag gaaggaggta aataatggca ggaaaaggac
    43741 aaccggcagg aaatatcaag ctagggatta gtttagatag cactagtttt ggtaacacgc
    43801 tggacgaaat caatgcgaaa gtcaaacaag ctgagtcgaa tatgcgtgcc aatctaaagg
    43861 cttatgattc agcaggacgt tcatacgaag cacttagtca aaagacgaaa gacttgtcta
    43921 cggttatgga agggcaaaac gccaaagtaa gagaattaac aaagcgccgt gatgaagcga
    43981 ttagcaagta tggcgaggaa tcgaaacaag ttgctaacct taacacacag ataaacaatg
    44041 ctaccgcaaa atataatgct tacagtcgcc agttgaacga cacaaaaaaa gaattggtgt
    44101 attccaaaac agccgtcaat gatttatcta atgaaatcaa agaaaatgaa cgacaaatga
    44161 acgccgaagt aaaagcgttg aaagccgctg gtgatgaatc tggtgctttt gaagcaaaac
    44221 aaaaagggct agccaaacaa acggaattat ccgagaaagc tatcgaagaa cagcgcaaag
    44281 ttgtgaaact gatggctgat gagtttggcg attcagcaaa tgaaaccgaa gatgcaaaaa
    44341 gggcattaga aaagttagaa cgacaaagcc aaatatctag caggcaatta gaagcactca
    44401 aaagctccag cgatcaatca ggaaaagaaa tagaagattt tggcgacaag tccacaaggt
    44461 cagctaggaa actggacgga ctaaaagaca aattaagctc gctaaaaagc gcattttcgt
    44521 ttggtgcagt tgctggatta gcgcataacg ctattagcag tgtagtaagt ggcgtgcaag
    44581 gtttggttgg cgaagcagta aacgcatcgg attcattgat gaagttttcc aaaaccatgg
    44641 agtttgctaa ctttgggaag tcacagatag aaagctcgaa aaaagaaatg aaagactacg
    44701 ccgataagac ggtttatggt ttagaagaaa ttctgaacac aaccgcacaa ttggcatcta
    44761 atgggattcc taactataca gaactaacca aggcggcagg taacttgaat gccgttgcag
    44821 gcggttctag tgatacattc aaatccgttg ccatgatgct aacgcagacg gcaggagctg
    44881 ggaaactaac aactgaaaac tggaatcaat tagcagatgc gataccgggt gcttcaggat
    44941 tgttacaaga cgctatgttg aaaaacggag cttatacagg aaacttccgt gatgcaatgg
    45001 cgcaaggtca aatcacttcc gacgagttca accaagcaat tgtacagtta ggtatgaatg
    45061 acggagcagt taaggcagcc acttctacag acacattgag cggttcttgg gagcagatga
    45121 aatccactgt aataaatggg ctacaaagta ttatagaaaa aataggcgtt gaaaatatca
    45181 ctggtttcat caacagagta acaaaaggga ttgaaaattc tattcctaaa attactcaat
    45241 ttataggttg gttgagagat attggaacgt ggatcgttga aaatagagag ccactaacat
    45301 ggattgtcgg aatcataggc ggaattacat tagcagtaaa agcattgaac gtagcaagta
    45361 tgttgctggc aattactggc ggaacattgg cagccccttt tgtggcgatt ggtgtagcat
    45421 taggcgcact agcaggtgct ttggtgatag cttatacaaa atctgaaaca tttagaaata
    45481 tagtcaatgc ggcttttaca gctgtgaaaa acgtagttat gagcgttatc aataatttgg
    45541 tggaatacta caaaatgttg tggagcgtgt tgcagtggct ttgggaaaaa ataaaagaat
    45601 gggcttcatg gattggcaat aaattcattg aaatgaagaa cagcgttgtg aatacggttc
    45661 agaacctatg gaatggcgtg aaaaacttct tcagcaatgg cgttggagat acgtggaata
    45721 aggtagtcgg ttgggtaaaa aacattttca acaaagcaac tgaattgaag aacaacgttt
    45781 ctgatgtaat cggtaacctg tggaatggta tcaaagacac attccgtaga ggtatcgata
    45841 cggtattcaa ttggttttca gaactaccaa cgaagatgaa gaatgccatt attggcggta
    45901 aaaacgccat tgttgatgcg ttcaaaagta ttttcaacgc agcacttaaa gcgataggta
    45961 aaccagttaa cgcaatcatc catggagctt catgggtact agaaaaattg ggcgcagaac
    46021 ctctacaaga atgggatgta ccacaatacg ctacaggtac accagcaggt gggcacccaa
    46081 tcaatggtcc aatgatggtt aatgatggac gtggagcaga aacagttatc acaccagatg
    46141 gtagagcctt cattcctaaa ggacgcaatg ttgttctaaa cgcaccaaaa ggaacgcatg
    46201 tcttgacagc agaagagacc gcgcaacttc aaggttctaa agctcctaag tatcgttaca
    46261 aaaaaggtac taacttcttt ggtaacatgt gggatagtgt gaaaaaggtt gctggtaatg
    46321 taggtcacac aattaaaaac gtagtaggtg acgtgtggga ctttatttca gacccgggag
    46381 cgttagctag aaaagtactt ggggggttag atgtattagg tggattgaca aagtatccat
    46441 tagaagtagg taaaggcatc ctatctaaag caacaagtgc actgactgaa aaaatcactg
    46501 ggttgttctc atctggtaac ttagatacct ccgtaggaac aaatggcgtc tataaatatt
    46561 tggctgatgt tgctaagtct gtgatgaaga aattcccagg atttgtggca actagtgggt
    46621 atagaccagg tgacccttat tcacatggta aacgtaatgc cattgatatt gcattaccag
    46681 gcgtcacagg aggctcacct agatacacag aagcagcaaa ctacgctttt gacaaattcg
    46741 cttccaaaat tggttacgta atcactaatg ggaaagttcg tgaccgttca ggacaatcag
    46801 gtcaaccagc aactggtgca tgggagccat ggcccgatgg agatcactat gatcatgtgc
    46861 atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc aggagatagc gtgggaggca
    46921 gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt gaaaatgacc ggtcaataca
    46981 gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac agagtcaaat ggtaatccta
    47041 atgcagttaa caattgggat attaacgcca aaaatggaac accatcaaaa gggttgctcc
    47101 aagtgattga cccaacattc agacagtatg caatgccagg attcaacagc aatatttttg
    47161 acccactatc taacatctta gcttcaatca gatacgcact atcaagatat ggctcactaa
    47221 caaatgccta tcgtggagtt ggttacgcaa acggtggaat tgtaaaccaa catcaaattg
    47281 cggaaatcgc agaaggaaac aagccagaaa ttattattcc gttagataag gctaaacgat
    47341 caagagcgat gcaattattg tctatcgcaa tggacaaact aggagtttct cctaaaactt
    47401 atggaaatgc gacaactgtt tccagtgatt acgaaatcct agaagccatt gaaaaacagg
    47461 cggcaacaac gaatcagctg ttatcgttat tgctggcatt tttcaaagga aatagccgaa
    47521 ctgatagaga tttagcttta gatattcaga agatcttggt taggaggatg tagatgcgaa
    47581 ctgtattact gagaaataga ataaacgaag aaattgattt gtccacagaa gactattttg
    47641 ctactgggtt gagtaatatg gggtttgaag taaaaaaaga acatgtggga cagtggggga
    47701 atttcagaga aagcagtgag agcgttgaaa tatctgaatt tcagtcatct gtaatcattt
    47761 ctgtgcatgg gtttcgagaa aaagaactgt acaattcact tgtgcagttc ctatcggaag
    47821 gtccgtttga actggaattt gcttttgacg gcgaaacaat ggtaagaaga tgcagtctaa
    47881 aatctttaag taagactgaa atcgatccga aaacatcgtt actaacagac actttagaac
    47941 tatatttcac atcaaactgg tattccgtaa aacgcgaaaa attaattcag cgaccgaatg
    48001 tagtgaaaac acgtggtaag gtttttccgt ataaaagatc ttatatctac actcaaaact
    48061 tgtgggagaa aaaaggtgta ttcaaattca ataataattc tgtataccta acgaatagca
    48121 aggaacggat gtccccgcta aaaattcgtg tgattgggaa gtgttcaaat ccgtattggg
    48181 aagtaatcca aaactcacaa atcatcgcaa cggatggata cttcatagat atgactgaaa
    48241 cacagactct agaagtatca agcctttttg aagatacaac ggcgatttta aaagatattg
    48301 caggggtaga atcttctgtc taccaacaac aggattatac aaaaactaat tttgttcagg
    48361 ccccaactgg agaattcagc attgtgtttc atgttggggg ggcagacgta gagattgagt
    48421 tatacgagga gcgtgatctg ttttgatttt agcagtcacg ctttttcatc gtgatttaat
    48481 gctttataac gaacatttat tctctaacag ttttgaattt ggtgtggacg agatcaacga
    48541 agaggctagt agctttacga tggataagta cgttcctgta aaaacaggcg attttctact
    48601 agcaaaatat attcctagtg gaaaatttgc atattttggt gtaattacgt cgcaggaaga
    48661 cgaaaaaatc agttgtaaaa gcttacttag tttagctgat agtgagatac cgactgcacg
    48721 tgtgtcagga gataactacg aagagcatat ccggcggttg attgaatact atttgctgaa
    48781 tgatccaacg aaacaactaa aagatatttt agacgtcaaa gcagaaagtg cgacctctca
    48841 ttcgtatcaa gctaccgata cgaataaaca caagttaagt gcgtatattc tcaatggttt
    48901 caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa aacagaaaaa tttatacagg
    48961 catacgtgct gtaaatgaat caatttacat taaagacaat tcttctgaat ttagcgattg
    49021 ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac aagctattaa tcgttgataa
    49081 agcaatgaaa gatatagaga aaccgataat actgtcaaca tggtatttgg acgaagaaaa
    49141 caatttgaca caagatggat cgaaagaaaa tatcacgaaa ccaactgtca atttggttaa
    49201 tatctacgat caaaccgcag aagataaagc atcctacgaa gatgtggcaa aatcagagtt
    49261 gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt gtaagaaatg caaaaaattt
    49321 gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt tcttataaag gaaagatata
    49381 caagtcggtt ttgacagctt ggcgaatatc aagtgataag gagtttgtgg aattgacttt
    49441 cggaaacata agaagtcgtt ttatggatta ttttgaagat aatgggggat aaaaaatggt
    49501 tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta agcgcagaaa atgatgctag
    49561 actttatcac gttttatata atcggaaaaa tcaggttatt gatggttacg atcagtctat
    49621 gaatttatct tcaagcggat taacagtaaa agttgctgca ggagcagcga ttattcaagg
    49681 tcgtatggtc gttgttcgac aagaagaaag tataacagtt ccagcaaact caagtggtta
    49741 tatagcatta acagtggatt tgacacaaga agttatacct ggatctattc ttccagaatc
    49801 ggaagaatat gaatggacta ataatcaagt caagctagaa tttataacaa aagttataaa
    49861 aggtaattta aacaatggtg ataaggtcta taatttacca ctatgctcag ttaattctac
    49921 tggatcaact gtttcaatct caaagatatc ggatagttac gagctgactc tctctaaagg
    49981 agaaattttg tggaggggga ctgcgttaat gcatgatact caaactgtcc aaccttcaaa
    50041 aaaaatttgg cagacagttt caggtttttt gttagtatgg ctcccatacg aaaacgggca
    50101 agcaattgag gatagatatg taactacgcc tttttataag gagcgtgtaa cttttactaa
    50161 tgttttagga gaaattgttt caggatttga tgactatcac aaaaagtggt ttagtaaacg
    50221 aataaactat aattcgaata ctaatatatt tacaggtgca gcaagcaatg caagtggaga
    50281 taacgcaaat atggtgctta ggtatatagt ttctttttag ttaggaggta tggaagagtg
    50341 gcaaatttag aaattaaatt atctgcaaat aagagacagc cttatctacg tcaccgtgtt
    50401 gttggtagag ttggtgatgg ggggctcaca acaatcaatg tacaacttct tgaggaagat
    50461 gaaattacac cttttgtaat taatctaaac ggtactttga aatttgtggg cgaagtttca
    50521 aacggtaact ataccgaggg agaaccagaa ataatcgatt cgactaatgg gttaattagt
    50581 tacacgttca ctaagtcaaa tttcagcacg agtcatcaat tcaaacaagc atattttgaa
    50641 tatgtagatc ctaacggcaa aaaagtaact tttcagaact tcatcataga cgtgttagaa
    50701 cgagtggata ttaattcgga gcaagcgaaa tactatattt cttcattgga aaaattacag
    50761 agtgaaatgc aaaccacttt caatcagttc attagtgata aacaggtcca atacgatcaa
    50821 atctactcga aatataacga attagtaaga ttgataaatg aatcagataa gcaagtaaat
    50881 gatcgtattg atcaaaccaa tcagcaaatc ggcgatctcg gcaagctgaa aaagatgtac
    50941 agtaacagca tcgactttgg gggctatgat tattcgggga atccgaattt gttaagtaag
    51001 ctatcatacg acttaattga aaatcaaaat acttcagctg gaacactttc taagggtgaa
    51061 aactcgttta aatataataa gatatcagct gaagtggaag gtggagtaga gttatattat
    51121 aaacgaagag gtatagctaa ctggttaccc tctaataaaa cgcttgtaat gactgttaag
    51181 cttagagctg gagcggacta tagtccagtt gacggaaaac ttatactgat tagatatagg
    51241 tatgttgaca gtggaactgg caagattgtt ttagacttac ctattaacag taattcgata
    51301 actcaggaat ggaaagagtt tagtattact ggaactactc caacatttag cccacaagca
    51361 taccatcctt ggatacaatt tagggctcaa gatggggtac ttggggaaat agaaatgagc
    51421 tatgacatca aaatcgaaga aggctctaca gctacaccat atcagcctaa cttacttgat
    51481 gactcttact ggctaggtaa aacaccgttg ggtgagaatt tgataacgaa tgccaagttc
    51541 ccaattataa cacgaagcaa tcctatttct ggttttgata tttcagaaga attaataata
    51601 ggtgaaactt atacggtatc tttaaaaggg actaagccag ctactaaaga attccactta
    51661 tactacggag aagcaaatta ccagcagttg cagtatcagg caactttact tcctgtggaa
    51721 ggattaacag atgtgtggag tgccactttt acagccagaa atactagtga aacgacaaat
    51781 ttacttagag tagcgttatg gcaaaaacct aattctgcaa catatgatac tgttcaaatt
    51841 gactggctca agattgaaaa aggcgacaca cgcacaccaa atatcagtca gtttaaatac
    51901 tttggtgaag gcttgaaaga cagtaacaat ccgaacgact acagctggga tgtcacacca
    51961 gagtatactg aaaaaagctt gaataatacg gttagtctga ctgaaccgca atccatcgaa
    52021 ggattgaaaa atttcgaaga tggtattcaa tcaaaaggaa aatctgtatt gacatcagac
    52081 gacaacaaat atgaagtcgt aaccttaaca gttacaaacg ggaataccgg atcagcaaag
    52141 ctttatcgtg aaggaaaaac cgtcactatt tatttttttg cgttgaatgg gaaaagcagt
    52201 ggtggaaatg attcaacgat actaacaatt ccagaaggct atcggccacc aattagtttt
    52261 gagcaactgg ttggctcgat agaccgttct actttgaaca gtgctcagtt atctattggt
    52321 gcagatggag ccattaaatg gcgaagaaac tcaagttatg gatcggatta tacctttgca
    52381 attacttaca cgatttagaa aggcgtgaat cgatatgaag gcagcatata gaccaattga
    52441 accttacgga ttcgagcaaa tcattgtgaa tgatgaagaa catttaccgg aagaatgcac
    52501 agaagtcgaa ccaccgattc caaattggaa accgaaattc aattactggg agggaaataa
    52561 atgaaaaaca tttggaaata tggccgtact ggcggagagt atgcaggaaa agtattggac
    52621 gacatgcttg tatccgttcc ttacacagat cagccaccgc tcgaaggggt tcgttcagat
    52681 ggcgaaccgc taacgattgc tgatcagatg tttgatccta aattgaacca atggattatt
    52741 ttagcgaacg cgttagatca caacgattta aacaatctca aagcgatgta cgaggctctt
    52801 gaacatgaaa acggcaacct aaaacagcta aatgccaaac tcatgctaag cgatgtagcg
    52861 attaaacagg aaaatactgc attgaaagaa aaagctgaca gtttagcaca aatcaattca
    52921 aaaatgatgc ttgcttcgtt acaaaatagc aaagacattt cagaaattaa agagcaacta
    52981 aatccagctt caaagggagg tgagtagtat gtttagtttt agcgatgtga aaatgatgta
    53041 tgattggggc tgttttactg acgatcaagt tcgtctattc gttccactat gtatcacaga
    53101 tgaagaagca gatagaatta ttagcaaaga agagagcgca tcttaagtga tgcgtttttt
    53161 tgttggaaag ttggtggaac atgaaagaag aagcgctcca agacgttgtg gagagattag
    53221 taagaattga aacaaaatta gacaactacg aatcacttag agaaaaagct gatagtgcaa
    53281 aagatttggc agacaaagcc tactcagtag cgctaaacaa cgcagaagat atcaaagaaa
    53341 tgaaagccaa taataaatgg tcgtggggtt acatgatcgg tttaggcatt acgatcattg
    53401 gctatttctt gactaaattg taaaggagtt aagaagaaat gattttaccc gataagtatt
    53461 atcaagtcat taaatggacg gttttaacag tattaccagc tgcatctgtt ttagtagcca
    53521 cgttaggaaa agcgtatgga tggaatggaa cagatatgac agtactcact atcaatgcag
    53581 tagcaacatt tttaggcgtt atcactggtg tgtctgcata taacttaaaa aaataggagg
    53641 aaaaaaatga agaagaaaat cattttatca ttgagcctaa taatggctct ttttttattg
    53701 ccaattaacg ggttcgccta tacgatcaac aatgaattta atttaggtgt aaatgaaggt
    53761 agctcacaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg
    53821 acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat
    53881 attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc
    53941 gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat
    54001 aaagtaacgt ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga ttccgctaat
    54061 aaatacggta ttccacttac attagacaca ccgtataatc aaccaggaat caaatcacat
    54121 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
    54181 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
    54241 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
    54301 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
    54361 aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg
    54421 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
    54481 gtaaatcaag cttataatac ttctggaaac gttggctatc atgtatcttt taacatgcgc
    54541 aatttcccta ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc agaaggaaac
    54601 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
    54661 taaaaatagc tcctcgttga ggagcagtac atagaattga aaattaacgt taaatcaaaa
    54721 aaatatttac aagatgttta tttaagtaga taataaaaga aaaaagcagg tgaaattgtg
    54781 acagcagaaa ttggtattat gaataaaagt ggaatcgtgt tagcttcaga ttcagcatct
    54841 acgattggag atagcaaagt atataatact gcaaaaaaat tatttacttt ggattccatg
    54901 cattctgtag gtatcatgat atatggtaat gctgaattta atggtattcc ttgggaaata
    54961 atcattactc aatataagaa aagcattggt agttctgttt ttaatacttt ggaagaatac
    55021 gcggataatt ttatagaatt tgtcaaaaca gcatctttta ttagaagtga acagaccgag
    55081 caagaacaaa tgattggggt ttttcaaaaa ataatttcag gtttatttga gagtatagaa
    55141 gtagatatta actttttaat aagccaaggt acacaaatag acaaagatgt cctagtgaag
    55201 ttattacaaa caaaaatgaa taccaatttg tctcaacagt cccagacatt cattttagat
    55261 attgaaaagg cgttatttct tagtaactat ggagaaatat taaaagatat tcttaatagt
    55321 atttctacaa tggaaggtgt ttcagaagct atatcagagg aaattcaaag ttacgtttat
    55381 gaaattataa ttagagacga tgtttattct tcccctacag gaatagttat agcaggatat
    55441 ggaagaatgg atattttccc taaactatat tcttataata tgtttggatt tgttatgaat
    55501 attctgaaat actcagaata tgaaagtgct caaataggaa atgacaatgg ttcattaaga
    55561 tctactatat taccatttgc tcagtctgat gtggtaaata ctgtagttca aggggtagat
    55621 ccacaaatta ctaattatct gtcgagtcaa gtcgatagtt ttgacgataa cggaaaaaat
    55681 acctatatta atattataaa gaatatttca gagtttcaac aaaatcaatt catatttccg
    55741 ttactaaaca tgattgcttt actcccagtt gaagaaacag caattatcgc agagacttta
    55801 ttaaatttaa ccagttttaa acgaaaatat actacttctg tagaaacagt aggtggccca
    55861 attgatgttt tagctattac acctaatgat ggccctattt ggattaaaag aaaacattat
    55921 tttgatattg ataataatat tggatacaga ttgcgaaagg agagagcgaa tgattacaat
    55981 aattaaacct caggaaaaat cccctgtttt gaaatttaat gagagcaatc tagcccataa
    56041 taaaatgagt ataactgata cagtatttga tgtaatggtt gagcaacaaa aaaaaattaa
    56101 taataacaat agacaaagaa aagaaattaa ccaatattaa tgttttttct gttgcgtttt
    56161 ttgcagtcat aatttaaaac ctcgctcaat cttgggcgag gtcttttttt atttttctga
    56221 aa
//
LOCUS       DAFCHQ010000009        54737 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000009 DAFCHQ010000000
VERSION     DAFCHQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.009"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            49..1008
                     /locus_tag="LNC71_000643"
     CDS             49..1008
                     /locus_tag="LNC71_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6465739.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1178..1858
                     /locus_tag="LNC71_000644"
     CDS             1178..1858
                     /locus_tag="LNC71_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6465740.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            1948..2982
                     /locus_tag="LNC71_000645"
     CDS             1948..2982
                     /locus_tag="LNC71_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK6465741.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            2996..3331
                     /locus_tag="LNC71_000646"
     CDS             2996..3331
                     /locus_tag="LNC71_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465742.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3315..5795
                     /locus_tag="LNC71_000647"
     CDS             3315..5795
                     /locus_tag="LNC71_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465743.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            5851..7410
                     /locus_tag="LNC71_000648"
     CDS             5851..7410
                     /locus_tag="LNC71_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6465744.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7407..8771
                     /locus_tag="LNC71_000649"
     CDS             7407..8771
                     /locus_tag="LNC71_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6465745.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            8942..10012
                     /locus_tag="LNC71_000650"
     CDS             8942..10012
                     /locus_tag="LNC71_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465746.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10027..10695
                     /locus_tag="LNC71_000651"
     CDS             10027..10695
                     /locus_tag="LNC71_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465747.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            10715..11293
                     /locus_tag="LNC71_000652"
     CDS             10715..11293
                     /locus_tag="LNC71_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6465748.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            11694..12605
                     /locus_tag="LNC71_000653"
     CDS             11694..12605
                     /locus_tag="LNC71_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6465749.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(12983..13900)
                     /locus_tag="LNC71_000654"
     CDS             complement(12983..13900)
                     /locus_tag="LNC71_000654"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBK6465750.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14102..14539
                     /gene="zapA"
                     /locus_tag="LNC71_000655"
     CDS             14102..14539
                     /gene="zapA"
                     /locus_tag="LNC71_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBK6465751.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            14597..15145
                     /locus_tag="LNC71_000656"
     CDS             14597..15145
                     /locus_tag="LNC71_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBK6465752.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15260..17620
                     /locus_tag="LNC71_000657"
     CDS             15260..17620
                     /locus_tag="LNC71_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBK6465753.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            17707..18021
                     /gene="trxA"
                     /locus_tag="LNC71_000658"
     CDS             17707..18021
                     /gene="trxA"
                     /locus_tag="LNC71_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBK6465754.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18203..19981
                     /gene="uvrC"
                     /locus_tag="LNC71_000659"
     CDS             18203..19981
                     /gene="uvrC"
                     /locus_tag="LNC71_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBK6465755.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20126..21025
                     /locus_tag="LNC71_000660"
     CDS             20126..21025
                     /locus_tag="LNC71_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465756.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21018..22280
                     /locus_tag="LNC71_000661"
     CDS             21018..22280
                     /locus_tag="LNC71_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465757.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22441..24339
                     /locus_tag="LNC71_000662"
     CDS             22441..24339
                     /locus_tag="LNC71_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBK6465758.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(24669..25388)
                     /locus_tag="LNC71_000663"
     CDS             complement(24669..25388)
                     /locus_tag="LNC71_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBK6465759.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            25725..26057
                     /locus_tag="LNC71_000664"
     CDS             25725..26057
                     /locus_tag="LNC71_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465760.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26044..28047
                     /locus_tag="LNC71_000665"
     CDS             26044..28047
                     /locus_tag="LNC71_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBK6465761.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28066..28536
                     /locus_tag="LNC71_000666"
     CDS             28066..28536
                     /locus_tag="LNC71_000666"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBK6465762.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28557..29141
                     /locus_tag="LNC71_000667"
     CDS             28557..29141
                     /locus_tag="LNC71_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBK6465763.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29156..30142
                     /locus_tag="LNC71_000668"
     CDS             29156..30142
                     /locus_tag="LNC71_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBK6465764.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            30132..30443
                     /locus_tag="LNC71_000669"
     CDS             30132..30443
                     /locus_tag="LNC71_000669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBK6465765.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30465..32246
                     /locus_tag="LNC71_000670"
     CDS             30465..32246
                     /locus_tag="LNC71_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBK6465766.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32239..33615
                     /locus_tag="LNC71_000671"
     CDS             32239..33615
                     /locus_tag="LNC71_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBK6465767.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            33622..34257
                     /locus_tag="LNC71_000672"
     CDS             33622..34257
                     /locus_tag="LNC71_000672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBK6465768.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34287..35642
                     /locus_tag="LNC71_000673"
     CDS             34287..35642
                     /locus_tag="LNC71_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBK6465769.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(35834..37228)
                     /locus_tag="LNC71_000674"
     CDS             complement(35834..37228)
                     /locus_tag="LNC71_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK6465770.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37320..38291
                     /locus_tag="LNC71_000675"
     CDS             37320..38291
                     /locus_tag="LNC71_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBK6465771.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38288..39070
                     /locus_tag="LNC71_000676"
     CDS             38288..39070
                     /locus_tag="LNC71_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBK6465772.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39239..39610)
                     /locus_tag="LNC71_000677"
     CDS             complement(39239..39610)
                     /locus_tag="LNC71_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6465773.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            39744..40187
                     /locus_tag="LNC71_000678"
     CDS             39744..40187
                     /locus_tag="LNC71_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBK6465774.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40230..41039)
                     /gene="yidA"
                     /locus_tag="LNC71_000679"
     CDS             complement(40230..41039)
                     /gene="yidA"
                     /locus_tag="LNC71_000679"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBK6465775.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41058..42425)
                     /locus_tag="LNC71_000680"
     CDS             complement(41058..42425)
                     /locus_tag="LNC71_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6465776.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42409..43239)
                     /locus_tag="LNC71_000681"
     CDS             complement(42409..43239)
                     /locus_tag="LNC71_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBK6465777.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43402..43839
                     /locus_tag="LNC71_000682"
     CDS             43402..43839
                     /locus_tag="LNC71_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBK6465778.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            43915..44535
                     /locus_tag="LNC71_000683"
     CDS             43915..44535
                     /locus_tag="LNC71_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBK6465779.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            44724..45230
                     /locus_tag="LNC71_000684"
     CDS             44724..45230
                     /locus_tag="LNC71_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBK6465780.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45247..46146
                     /locus_tag="LNC71_000685"
     CDS             45247..46146
                     /locus_tag="LNC71_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBK6465781.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46233..47117
                     /locus_tag="LNC71_000686"
     CDS             46233..47117
                     /locus_tag="LNC71_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBK6465782.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47309..47380)
                     /locus_tag="LNC71_000687"
     tRNA            complement(47309..47380)
                     /locus_tag="LNC71_000687"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47345..47347),aa:Arg,seq:ccg)
     gene            47440..48774
                     /gene="rpoN"
                     /locus_tag="LNC71_000688"
     CDS             47440..48774
                     /gene="rpoN"
                     /locus_tag="LNC71_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBK6465783.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            48923..50014
                     /locus_tag="LNC71_000689"
     CDS             48923..50014
                     /locus_tag="LNC71_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBK6465784.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            50055..51056
                     /gene="gap"
                     /locus_tag="LNC71_000690"
     CDS             50055..51056
                     /gene="gap"
                     /locus_tag="LNC71_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK6465785.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51166..52356
                     /locus_tag="LNC71_000691"
     CDS             51166..52356
                     /locus_tag="LNC71_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBK6465786.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52460..53215
                     /locus_tag="LNC71_000692"
     CDS             52460..53215
                     /locus_tag="LNC71_000692"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBK6465787.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53331..54629
                     /gene="eno"
                     /locus_tag="LNC71_000693"
     CDS             53331..54629
                     /gene="eno"
                     /locus_tag="LNC71_000693"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBK6465788.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        18194 a         9540 c        11542 g        15461 t
ORIGIN      
        1 ctacactcaa aatagttccg acgaaagaat tataaggagt gagtacaaat gacctataac
       61 catcttacac caactgaact cgtaatgata gaagcttatt tcaatcaatc acaacctgtt
      121 tcaaaagtag ccaatctgtt acaacgttct cgacaaacta tttataaggt ctatcgtttt
      181 cttaaatctg gtggaagcgc aatgagctac tataaacaat ataagaaaaa caaacgtaac
      241 tgcggaagac atccgattgt tttacctgat gagcaacagg aatacattca aaaaaaggtt
      301 gttcaagggt gggctcctga tgtccttatt ggtcgagcag agtttcctat tgcttgttcg
      361 gtacgcacac tgtatcgaat gtttaaagat aaaacctttg acactcacga tttaccaatg
      421 aagggaaaaa gaaaaccaaa cggtcataaa gaaaaaagag gaaaacaagg gtttcgccgg
      481 tcaatccgag acagaaagac agactatacc cagtttgatc atgaatttgg tcacctggaa
      541 ggtgacacta tcgtgggtgg aaaacataaa agcgcggtca tcacattagt tgaacggcta
      601 tctaaagtca tcattacctt gaaacccgaa ggcagacagg ctaaagacat tgaaaatagg
      661 ctgaatcaat ggtttcgttg tgtcccaaaa aacttattta aatccatcac atttgattgt
      721 ggaaaagaat tctcaaattg gaaaaagatt agtaatacca acgatatttc aatttatttt
      781 gcggatccag gaacaccttc tcaacgagga ttgaatgaac attcaaatgg cttattacgt
      841 aaagatggtt tgtataaagc tatggatttt aatggtgtat ctgaaacctt tatccaatcg
      901 gtggcatcga aacgaaatca tattcctaga aaatcattac actatcggac accaatggaa
      961 gtatttttga gttacgtcac aaaagatgat ttgtctaact taatttgaca aacgaaactt
     1021 aataaaaaag gaagaatatt catgtaaacg attttattgt ttttttctct tttatacaaa
     1081 aagggtcata atttcaagta agcggatgaa ttcatgagga ttttttgcat actgtctcta
     1141 ctatacttag gtcactaaga aaaaggagtg atttattatg aaaaagaaca tgattgggac
     1201 attattactt tcaacacttc ttctaggcgg cttggcaaca ccagcttttg ctgagggtca
     1261 agcgacttca aaaggagata ttacctttac tgaaccaaca aataccgtgg agcctcttaa
     1321 ccctactgat ccttcaaaac cagtagagcc agcagatccg gaaaatccag caactggcca
     1381 gacaggtcct ttaacactgg atgtagttcc cgaattgcca tttggtacac acgagattga
     1441 aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac gatacacctt atctccaagt
     1501 atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa gggtggaacg tgacagtttc
     1561 ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc gctgaattag attttggaac
     1621 atccactgtt aaaagtacgt cagataacga atccactgga ccaactagtc aaactgtaac
     1681 tgggttgagt aaagcaagtg cagcgacacc aatatttaca gcagcaaaag atcaaggtct
     1741 gggaacatgg ctttctgttt atgatccagc aaatatcaca ctgaaagtgc cgaaagcagc
     1801 agctggaaca ttcacggctg atttgacttg gaacctcgtg gctggcccag tagcatagag
     1861 tatgaaataa attcaacaaa ctgcggtgaa aatactatag gtgaaggatt ttttaccgca
     1921 gttgttgttg aaaggatgtg gtggacaatg aatcaaagta aacgactgtt tgtttcgttt
     1981 ttttctattt taagtctatt tttcattttt ccgtcaatca gccaagcaaa ggattcagca
     2041 ggagactttg gaatcaaacc ggtatttcca gagaaccaaa tagataaagc aatcggttat
     2101 tttgatttat tagtggcacc ggaacaaaat cagacactag aagtaattat cagcaattct
     2161 tcagatgaag aacgaacatt tgaggtctct gtcaatccgg cggtaacgag tgatggtgga
     2221 acgatcgatt atagtcaaaa aaacccaacg ttagatgaga cattgccgtt tgatgtccgt
     2281 gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag cacatgcaga aacaactgta
     2341 cctatagaag tgaaaatccc agcaaaaagc tttaagggac gtgtattagc gggtattcat
     2401 gtttcaccta aagaagaagc tgaaacagaa aatgctacag aaggagcaca aatcaaaaat
     2461 cgtattgctt ataatctcgc agtcgtttta caagaaagtc aagagacgat agaaccggat
     2521 ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa agccaactgt tcagcttcgt
     2581 ttccaaaatc ctcagcctag gattatcagc aatttgatat ttacgagcaa gatcttttat
     2641 gaaaatcagc tttatattga aaatacgtct aatgcatttt tagttgcacc aaattcgaat
     2701 tttcacttga atcttgattt agctggtgat aaagcaaaag ccggcgatta ccgcgcagaa
     2761 atcatagcaa aatcaggcga cagtaatgaa tggcgattta cacaaaactt tacgatcaaa
     2821 aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg cagtacaaga acaatctttc
     2881 ccatggcttt atgcaggatt ggttgctgca gttctagtgt tatggttgat tttgctgttt
     2941 ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat gatgaggtga tttcgttgaa
     3001 aacatcgatc ttattgggag ggattattgc aagtttgctg ttcttcagct ggcaagagcc
     3061 agtccaagca gataccaaaa gttcaggtac gattacattt tatgatctaa gtccaattcc
     3121 gcatccaagt ggttcagtca atggcgcatc aaaacagcag acacagctac cgatttataa
     3181 agaatctaat caacatggcg tattcccgga gacgaatgaa caaaagatcc gttttcttta
     3241 tttgatcatt ggtatagaat tatggcttct tgcgtggttg ttgttatgga gaagaaagga
     3301 aaaggaggat gaacatgttg ctacaaaata aattttcatt ttttcgctcc ttctttttta
     3361 cttcgctttt tgttttaagt atatttatct tttcaccttc ggcagaagct gttggagcaa
     3421 cgcctcctgc catttcttat gatccataca cgtatgaaaa tatcgtacct ccaaatggtg
     3481 gacgtagtgc agtcaatggc gggaagcttg tccaactaac tggggctaac caattgccta
     3541 aattgactgc tacttatttg aacgatggtt caaatgatcc ttggaaaggt ggcacggtcc
     3601 gttttcattg ggaaagccat gtgaaactaa caaatgtact tgatttggtg aatatcactg
     3661 cacaaggatt tgcatttgtg gcgatcatca atttgccgga acatgtatca agcagtgatg
     3721 tattaaaagc aattgactgg aacaacgcct acttgtatat tgccggtgcc cccatcaagg
     3781 ttactagcgg aagtttatta tcagcaggaa gccatacctt gcgttttggg atggggtcgt
     3841 gggatacaaa tactctattg acagatatta ttgctattat tacgcgaggc gaattcaacg
     3901 tggacgatat cccgcttcaa tttgatgtta cagctaatgt cgcgaagatg acagaaaacg
     3961 gagataaata tgatacgagt ccaaacaaaa ttttgaccaa gggtaaatta caaccttcta
     4021 tgtctaaaaa ggccgttttt tctgttgatt tttatgattc gaataatatt atccaagatc
     4081 gtatgttcaa ttcgatttta gctccagctg caggtagatt ttatcctaaa ttaactggcg
     4141 gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc agagataaat acgtggaata
     4201 gttacatctc accaagtgat tcaacaggaa aatataatac atttgctgat accacagaat
     4261 acgtggatgg aaataaccag aaccttccag gctctaccaa tagtcggaca ttgacgatgg
     4321 ggctgactga aggacgtttt gatcagttgc catttagtcg ctttgatcgc gtagtcaact
     4381 attttacagg aaaaaacgtt acacaaggag caatactgac tcatactcca gcaagcacaa
     4441 gtggattgaa tcagacagcc aatatcgtgt acagtgggaa agatgcgcaa ggcggattgc
     4501 tttctcctgt tgctttaaag gtattggagc agtacaagct agacggaacg gttttaccaa
     4561 caaacttaac agccgatagt cagtggggaa agctcctttc ttctgcatcg taccaagtca
     4621 cggccaattg gtcagcaact gaactaaaaa agggttcgat ccattacagg ctttattcga
     4681 aaggaaccca aaaattagcg acaggatgga cagatcaact tttccaaacg atccaatcaa
     4741 ataacggggg tgtaaataca gctacttcta cactacctgc attggcttca gggcaatatt
     4801 attttgatta tcgcttggtg gacgatgtac ttactaacat gtatccgtct tttgcgtaca
     4861 aatggcagtc tgaacaagct ggaatcacca ctcttcctca aataacagtg gctaattttc
     4921 catctgtgag tgcagatagt cagctaaaaa accttagcag acaacctgag acaggagatc
     4981 ccctgtctgc tctatcagga gatacgattc aagaaaatgt gacattcacg ttaacaaaat
     5041 ctggggatgc gataaccgac aaaaaaatca cgatctcact tcctgaaaat actacttata
     5101 tcaatggttc cttaaaatta aatggtacaa ctatcgcaga taccggcatt caacaaggag
     5161 tttctgtccc agcagatcta ttgagcaaaa taggagatac cattcatctt acttacaact
     5221 atcaattaaa tactgtagat acttctttac aatccgtcag catactgaca aaggcagctg
     5281 tactttccag caatattaca ttagcagatg gtgctaagct tcctaaccca gttgtccaaa
     5341 catctgctaa aacaattctt gtccccaaac aagaattaac attagttaat gtaccagatg
     5401 attttacatt tggtaacgat ctccccaaac cgttgaagac aagttattat gaatcaaaag
     5461 gcgatttttc ttttgatgtg cgggatactc ggcttccaag cacgtctcct tggcaactga
     5521 caggtacatt gacttcttta tttaagaatg atcaaggtca agaattaagc ggaacaaaat
     5581 tatacttcaa tcattctgga tcaaagcaat tgattcagca aggtcaaaac acgttgattt
     5641 atgagagtga tggaacagct aaaggagagg ttctcgttga ctttcccgat accgatggtt
     5701 tactcttaga ggtaaatagc agtacgaatg ctcaacctgg cgcaacgtat caaggcatgg
     5761 tcacatggga actgacagct gggccaacca gctaagtgat tagaatttgt atggatctgt
     5821 gataactgtc acagatcctc tttttcaagg atgagaagga aacttctgct gttaaaatca
     5881 gaaatgcttg aaggagtgga cttggaactt ttcagtttgc ttgaaaaaaa tgaacaacgg
     5941 caaatcaaac ttttccaact tttattagag gaaaaaggag aggtttccgt taataacgta
     6001 cttacccgct tatcagttga tcgggctacc ttgaaagaag atctgtttta tttacaagct
     6061 aacttggaaa atatcaaaca attggaaatt gttatccaag aagggaatat tttattaatc
     6121 agacatggaa atatagcagc agtcgatgtt tattatcatt gttacttgcg ctattctaca
     6181 aaatatcaga tacttcttta tctgttggaa aatggctcat ttgatcggca gaaacttgtc
     6241 atggaactta atatcagttc ggctactttg tctcgcagag tgaaggaatt aaatgattct
     6301 ttaaatgagt ttcatctgaa aataaaaagc gggcggctga ccggtccaga aagtcaaatt
     6361 agttattttt atttccaatt attttggttt ggacgtccct atcttgaaaa caaaaaagaa
     6421 tacagtgatt tatcaggaaa cactttttta gatctgctgc aaaaagagtt tagttttgct
     6481 attacagaag aaggacgatt gaaatttgaa atatggcttt ccattatgaa gaagcgaatg
     6541 cgctgtacga aaaaatccaa accagattat agcaatcttc ctgttggatt gtttcaaacg
     6601 acacaatttt tattggacct ccatacattt ttgacccgtt acttctttca atatgccttt
     6661 ccttggaatc aaaatgaaac gttggttttt tatcttttca tgatcagtaa ctttacatta
     6721 gatacacata actcgcatgt ccaggagttt ttgataagcg aaaaatacaa agacgacgta
     6781 gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag aatatttctc tttttatgtt
     6841 tttccagaag agatttcgca aaagattgcg ttgacagtga tgcaaaatca cgctcatttt
     6901 gcctatttta gaggatggat gaatattttt ggccaagaag gattaaaaca gcggttaact
     6961 gaagtagaag ataaacaatt cctggtttta tgtgaaagat tggtcagaca aacatttgct
     7021 attgttgatt ttactgaagt agacgaggaa aatgcccgct ttgaattatt tgctcgctat
     7081 gcatctattt tacggacgct gatcaagcag ttagaattac gtctgcaaat tgcttatgat
     7141 cttccttatg aaaggctaat ggcggatatc gtgaaagaaa tcatccagga acaattgctg
     7201 aatacgatca aatatgattt agtcgaatac gagaaagaaa agcagtatga tatcattttg
     7261 actagccaat tgaaagagta cccgtcacag aagtccgcta aagtttttgt attcactagc
     7321 aacgaatata attatgattt cccctatttg aaccaatttt taaaagaatg ttatttagaa
     7381 aaattgaatt taaggatgaa taaaacatga aaatcgtgat cattggtgct tcccatgaag
     7441 gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa agcagaagtt ttactgatcg
     7501 atgaagatca taaagatgga ctaggctatg tttcaaatgg aatcaacctt tatttaaaag
     7561 gaaagatacg tagtttagct gaagttgccc ataacatgcg gactcttcaa gaaagtgggg
     7621 caaaactgat caccgaatgg agagtaactg aacttgatcc agacaaacat cagctcatcc
     7681 tcgcatccaa agaaggaaaa aacgagacca taacctatga taaattaatc gtagcgacag
     7741 gttcctctcc tgtcaccttg tataaacaaa ttgaagctga aaatgtctac acctataaaa
     7801 atctagtgca atctaagcaa gtacttgccg ctttaaaaga agcaaaagaa gtagtgatct
     7861 tcggtgctgg ttacattgga ttggaattag cagatgcttt gagaaataaa ggatacatta
     7921 ttcatctagt tgattatatg ccgaatgttt tatcaagata tttcgataag gatatgatca
     7981 acagttttca aaatcagctg caaactaagc aaataaattt ttatccaaat gaattcttga
     8041 tcgattggaa aaaatcagaa gagaaagtag tatccgtaca acttctgtct caaacaataa
     8101 aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa tacgacattg ttaaaagaaa
     8161 aagtggcttt gtatgaggat gagactgtga tagtcaatga atatctgcag acaagtgatc
     8221 cggacatcta tgcgattgga gatatcgtcc cagtcagttt tgacaaaaat aaacggcatc
     8281 tctttttacc tttagttact cgagctgtac atatggcaag agcagtagcg ctaactttga
     8341 gtggtcagcc aaccgcttac gacctcagac aaaagataac agcagcagtc attatagact
     8401 actttttagg aactgtaggg ctgacagaag atgaagcgcc attcttggaa caaaataccg
     8461 gttcgtgttc aggagaattt gatctatttc cacaatacga cgaagaaaac aaaacagtaa
     8521 atgcaaagct gatctaccat ccggatacat tagaaataat tggcggacag ctgataagcc
     8581 aagaattctt attatcagat ttgaacctgc ttgcggatat cgtgaaacac aaaactacga
     8641 ttccacagct agcagttgag gattttggat ttctgtctga atacacctct cgttttcact
     8701 acctcaatga gttagctttt aaagtactag ctaagagagc aaatagcaat agagtagata
     8761 aaagactgtg agaaatatca cggtctttta ttgtgtgggg atataataaa tattaatcaa
     8821 tcgagaataa aaaattcata taatgaaata gcggaagaat tttttgagca taatattcgc
     8881 caaaaattta tcaagtgata aaattgttgt ataaaatgat tatttcagga ggaggagtta
     8941 aatgttttta gcattaaatg aaattatgca ttcaaagtta cgttatgcgt tagttgcagg
     9001 ggtgatgttt ttgatcgcct acctggtatt ttttttgact gggttagctt atggcttagc
     9061 tcaagataat cgaacagcag tcgacaagtg ggaggcagat tcgattgtgc tttcgaaaga
     9121 tgcaaattca aatctaggga tgtccatgat tactaagaag atagcagaag aagtcgaagg
     9181 tgggaaagtg gcgtatcttg cccaaacacc tggagtcgtg actagtaaag acagtacaga
     9241 agaaggaaaa atcaatgtta gcttcttcgg tatcgataaa aatcaattca tcatgcccaa
     9301 tcttgttgaa ggaaaagctt ttgataacga tgatgaggca gtaggcgata ttagtttgaa
     9361 agaagaatat ggtctagcca taggagatac agttaagtta tctggtagtg acaagacgtt
     9421 taaactaact ggctttacgg atcatgccaa attcaatgtc tcaccagttc tctatacaac
     9481 catcaatgct taccaagaaa tccgcttcga aaaagaagat acaagtgaaa atgcacggat
     9541 taacgcaata gttgtcagag gtaaaatcag taatttacct gaagatttag aacagatcaa
     9601 aatttctaaa ttcatcaatg aattacctgg atataatgcg caagtattga cttttggatt
     9661 tatgatcgga tttttgatcg ttattgcggc tattgtgatc gggattttca tttatgtact
     9721 gacgatgcaa aagattaata tctttggtgt aatgaaagca caagggatca caggaggatt
     9781 tattgcgcgt tctgtagtgg cacaaacatt cattttatca tttgttggta ttcttcttgg
     9841 tctattagga acagtgggaa catcccttgt tctacctgat gcggttccat tccaaagcaa
     9901 ttggctgttt tttggcgtca ttagcttgtt gatgttagta gtagctgtgt taggcgcact
     9961 attttctgtt cggacaatcg taaaaatcga tccgttgaag gcaattggat aagaaggagg
    10021 aagaacatga aagcaataga atttaaagaa gtagaaaaaa gtttccaaga tggcgaccaa
    10081 gtagttaaag cattgaaaga aacaaccatg agtatcgata aaggggaatt tgttgcaatc
    10141 atcggtcctt ctggttcagg taaaagtact tttttgactt tggcaggcgg attgcagacg
    10201 cctagcagtg gctctgtatt tatcaatgat caagaattca gtaatcaaaa agaaagcgaa
    10261 cgcgttaaac tacgttttaa agaaattggc tttattttgc aagcttccaa tctggttcct
    10321 tttctgacag taaaaaagca gttattatta gtagataaga ttaaacatga acatcgtcaa
    10381 aaagaagcaa acgaattatt tgagcagctt ggcgtggata agttgatcaa caagtatcca
    10441 gaagaactat ctggtggaga acggcaaagg gtcgcaatcg ctcgtgcatt gtacaatgat
    10501 ccgtccatta ttctagcaga tgaaccaact gctagtttgg attcagaaaa ggcacgagaa
    10561 gtagtcgata tcttggcaaa agaatcaaaa gacaaaaata aagcaacgat catggttact
    10621 cacgatacac ggctgattga caaatgcgat agaatattta tgatggaaga tggtgtattg
    10681 aaagaaaaag aataaccaaa aagcaggtgg aaaaatggtc catcgataca acaaagatac
    10741 acagcaaaaa attttgaaag aagcgactga cttgtttatg tccaagggat atctcggcac
    10801 ttcaacaaga gaaatcgcac aaaaagttgg gatcacccag cccaatctct atcattattt
    10861 cggtgataaa gaaaaactat atacggcagt tttagaaaac cacttaaaag atgttggaaa
    10921 agctttgaga gaaatcgttc aaacaagcga aacgggattt caagtaacct tgacaaaaat
    10981 ggcgcagttt ttgattgaga cacatctggt cgatttgttt ctgatgcttc acgatcttga
    11041 aagcaatctt tcaaaggaaa caagagatca tcttttcttt ctatggaaga aaaattatcg
    11101 tgaacctttt gaagaaatct tttcagcaaa ccaatctgtg ctaagaaatg ggatttcaaa
    11161 agaaatcgca gcaaggcatt ttttcttagt gttagcacct tacatcactc ggtcgaaaag
    11221 acctgtggaa gaaccgctaa atgtggcgca attgatcgat ttatatttga atggtgtatt
    11281 aggagaagcg taaagctgtt atatagggag aaaatagtta ggtgaagaag caagatatga
    11341 cgaagatcgt tatatcttgc ttctttatat aggtagtttt tcataaatac aaatttgtta
    11401 ttctctcatg attatcaggg tatattttta tttattatgt cggtgttcca ggaagtgttt
    11461 taatttaata acgaagaaat taattgtttg tagaatgaag gaaactattt attgggtaga
    11521 tgaatcacca cgaatagtca aaaggagatt tcaggatatg ccaaaaatga aaaaaatcat
    11581 tttagcagta atgttatttt ctacggttat atcttttaca gccccttttt tgcttactta
    11641 agctgatttt aagttttggc ccttagtctg gatctacgag gtgagagtca agaatgaaaa
    11701 aaataattgg agcttttgta tttgcaatca ttatcattat tatctgtttt caaatagatg
    11761 tttttggtca aaaaaagata gttggacaaa cggtcagcag tacgaacagt acggttacaa
    11821 agacaaagga atcatttgat gatacagaac aagagacatt aatcaaaatc ggaaaaaatg
    11881 accgtacact atatacagat gactcgctaa ctaccccact tgcaactata aacggcggag
    11941 agctggcgga gtttctttct gaaacaaaaa atgcttatca gatcaagaca aatgatggct
    12001 atactggata tttagccctc gaagatggaa ccaaagtcac aaagaatata caaacaaaac
    12061 cggaagagct atctgatgca gtgattgtat tagatcctgg gcatggcgga aatgatactg
    12121 gggcattaag caatgatgaa caaacagaag aaaaagatat aacattaagt acagccataa
    12181 aagtaaaaaa ggcgctagag gatgctggag caacggttta tttgacccat actacagatg
    12241 aattagtaca gcttggagat atatgcgact acagtgaaga aaaaaaggca gatgtcttta
    12301 tcagtttaca cgcagactct acggaatatg cgaatgaagc aactggaatc acgacatatt
    12361 attactatgg acaagaggaa acattagcac agacaattgc tgactccttc actgatctgc
    12421 ctctggattc aagaggaatc tccactggga actatcaagt tcttcgagaa aatcttcaac
    12481 cttctatttt aatcgagatg ggttatatga ataatgatag tgatttaaag gaattagtaa
    12541 cagacagtta tcagcagcag attgctgcaa gtttgacaca agcattgacc acttattttc
    12601 agtaataaat aagcccagcc aaacaaaaat agctcctcat atttttcgtt tggccgggct
    12661 tattatcgca gatgcaagct tttaaacacc gtttaaggaa gaggtcgtga gcctgccgtt
    12721 caactctgag tcacaaggag caatttcaaa aaacagcttc tcatgtttga tgactattgt
    12781 gacttgtgac cgaggagttg gcatgagaac accgtttaag aaaaaggttg tgaaatcaac
    12841 gttttgttct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttg
    12901 ttgagctaat accgaggatc agatgattga acaccgttta ttcggaacaa gggactgtga
    12961 catgactttt gtcacagttc cattattttt ttagtagctt caaagctttt tctgtattgg
    13021 caaaatgaag tttggcatat ttttcaagca taggcatgcc gcctttctcc aatagttttg
    13081 ccgctacttg gtcagatttc gttccagctc ctgacggaag ggtgattcca gcttcagctg
    13141 atgctttgtc taactcttcc aaaaagctgg cacgactgat gatagaagca gcagcaacag
    13201 ctaaatgata ttgttctcct ttagtaacga agtacagttt ttcttgtaat tgattttttt
    13261 ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa ctgatcgatc aaaatcgctt
    13321 ctggtttttc tggctcgatt ttttttagca gcaaatggat cgcttgattg tgcaaagcga
    13381 ctttcatccg aacggcattg tattttggtt ggatctcatt gtatttttct ggagataaga
    13441 caagcagttt ataggggatg gtttttttta agatgtcagc aagctggatg atttggctat
    13501 cttttaactc ttttgaatct ctaacaccaa gtttacgtaa ttctgcaagc tgatcacgtt
    13561 tcacataagc agcacatact gttagcggac cgaaatagct gccatttccg acctcatcac
    13621 tacctaaaac ggataattga ctgatatttt ttggcaaaga agaggctgtt tttgctggct
    13681 tggcttgttt atcctgtgcc tccccccatt tatcagcttc tattgctgca ttcgtacctt
    13741 gaaacatcac ttttccggat gtataagcgg taatggtcgt cccgtctttt tttgcagcaa
    13801 agctagtata aggaatctgt ttatttaaca aataagagcg atagtatgct ttcatttgac
    13861 taagttggtc gtttgttgct ttgataatga cgttgctcat aaaattctcc tcgtttttat
    13921 acataaaact ataccgcaac tcacgacttt tcgttttcta agagattgcg agacatagca
    13981 tttttttaaa gtttatcata taatagttaa gtatgtgaaa aatatttgtg gagaacggtg
    14041 aaagaaccaa cttcatggta cactaaataa aactttcatc aaataggagg aaatgattcg
    14101 tatggcgcac gaaaaaacac gctacaaagc ggtgattgcc aaccaaacat atactattat
    14161 cggacgggaa accaagcatc atatggatat cgtaacgaag ctgatcaatg aacagttagc
    14221 agaattgaaa caattatcac cacaaatgga taatgaacaa gcagcaatct tgatggctgt
    14281 caatgcgctt tcggatcaat tgaagaaaca agaaagaata ttggagctgg aagaagaaac
    14341 agcagaatta aagaaaaaaa tgatcaaatt tacagagctt gaaaatcgtg tgaaacgcat
    14401 tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa aatggtcaag cagatcatac
    14461 cattaaaaat catgtggaag cacaacagat tttaaacgag aaacgaaaag gacaaattaa
    14521 acaaaaatct tctaattaag atccttttat caaaataaga aaaatttaaa aaagttagaa
    14581 gagaaaggaa cgtagcatgc tgagtttgct gattttattt ttattgctaa ttgcgttttt
    14641 ttctggagca cgtcgcgggt ttgctttgca aatcatattc gcgattggat atgtattgtc
    14701 atttattgct gcccagcatt tttacaaacc attagcaagt catttggaat tatacattcc
    14761 atacccagct gtcaccccaa catcaaagtt agcatttttt gatcaggtat ttgcctttca
    14821 cctagatgaa gctttttatg caggaactgc ttttctgatt attttgctaa ttggctggct
    14881 gcttacccga tttgtggggg tttttgtcca tggattgacg tatgtgccga tcttgagaca
    14941 ggtagactgg attgcaggag gcatcctgtc actaatcatg gcttacgtta ctatattttt
    15001 gattttgcag ttacttgcct ttgttccatt agatgtggtc caaaatcaat tcggcgagca
    15061 tagtttcgct cgttttatcg ttgagagaac gccatttctt accaacaaga tgtatgagct
    15121 ttgggtaacg aatattttga attaaggaaa atagtgctgg aaaatatttc gttttttgta
    15181 cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg gcacagctct tttttcatag
    15241 tgaaagataa ggtgataaaa tgaataaccg aatccttgaa acattggaat ttgaaaaagt
    15301 caaacagatg gtcagacaat ttgtagtgac tgcacaagga aaagaagaac tggcagaatt
    15361 ggtaccagtg agtgagaaac aaacgatcac aaactggctt caagaaactg aggatggatt
    15421 gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa attgaaaata tccgtcccca
    15481 tatgaaacgg attgagatcg gagcagactt gaatggagtg gagcttgcac aagtttccag
    15541 agtattgtct acgacaagcg aattgaatcg tttcattgat gatctttctg acagtgaaat
    15601 cgaatttgca cgtttgtata tgtgggcaga tcagttagtc acgatccctg ttttgagccg
    15661 ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact gatgacgctt ctcctgagtt
    15721 aaagagcata agacagaaca ttcggcgaag tgaacaagct gttcgtgaac agttagatgg
    15781 gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg atcatcacca tgcgaaatga
    15841 ccgctacgtt atcccagtca aacaagaata ccgcggtgta tttggcggtg tagtccacga
    15901 tcaaagtgct tcaggtcaga ctttatttat tgaaccaaaa caagtggtgg atttgaataa
    15961 tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt caacgtattt taagtgaact
    16021 ttctgctgaa cttgttcccc atcgtcaaga aatcatccac aatacttatg tgattggtaa
    16081 aatggatctg atgaatgcaa aagcacgatt tggtaaagaa gtaaaagcca tcgtgccggg
    16141 aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga catccactga tcgatcagga
    16201 aaaagtagtt tctaatgaca tcacaatcgg aaaagactat caagccatcg tcattacagg
    16261 acctaacaca ggtgggaaaa cgatcacgtt gaagacttta ggtctgttac aactgatggg
    16321 gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa atcggtattt ttgaggaagt
    16381 atttgcggat ataggagacg aacagtcgat tgagcaaagt ctatcgacct tttcttctca
    16441 tatgacgaac actgtagata ttttatcaaa agtcaatgaa aaaagtctgg tattatttga
    16501 cgagctcggt gcaggaacag atccgcaaga aggtgctgct ctagcaatcg caatcttaga
    16561 tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg cattatcctg aattgaaagt
    16621 ttatggatat aatcgggcaa atacgattaa tgccagcatg gagtttgatg tggatacatt
    16681 aagtccaacg tatcgtttgc tgattggcgt tcctggacga agcaatgcgt ttgaaatctc
    16741 tagccgatta ggtcttgata cagaagttat tgatgaagcg aagcagttga tgaatgatga
    16801 gagccaagat ttaaacgaaa tgatcacaga tttagagaat cgccggaaaa tggcagagac
    16861 cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa gaacttcacg atgatttgaa
    16921 agaagcttac agctatttct ttgaagaacg tgaaaaagaa atggaaaaag caaagaaaaa
    16981 agcaaatgaa gtcgtgtcag aggcggaaga aaaagcagaa aaaatcattg ctgatattcg
    17041 taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag catcagttga tcgatgcaaa
    17101 gacccaatta gcaaatctac atcaagaaga aactttgaag aaaaataaag tattgaagaa
    17161 ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg ttggttacga cttacggcca
    17221 aagagggacg cttttacgta aaaacgggaa tcagtggcaa gtagaaatcg gtatattaaa
    17281 aatgaatgtg tccgaagacg agctgacgcc tgttgctcct caaaaagagc cgacacaacg
    17341 agttatccat gctgtacgat ctgaatctag tagtcacgtg ccgaatcaat tggaccttcg
    17401 aggaaaacga tacgaagaag cgctaagtga ggtagatcaa tatttggatt ctgccattct
    17461 agctggttat cctcaagtga ctattgtcca cggaaaagga acaggcgcac ttcgcaaggg
    17521 aattactgat tatcttaaaa accatcggag cgtcaaaagc tttgagtttg caccggcaaa
    17581 ccaaggcgga aatggtgcga caattgttaa atttaagtaa agcttactaa aggtaagcag
    17641 atggttcgct atccaagtat agaaatgcta tactagtcat agattttaaa aatggaggtc
    17701 tttgatatgt cacaagtaat tacagataag acatttaatg aagaaacaga taaaggactt
    17761 gtattgattg atttttgggc aacttggtgt ggtccatgtc gtatgcaagc accgattttg
    17821 gatcagttgg aacaagaata tgatgaagaa gaattcagaa ttgctaaaat ggatgtggat
    17881 gaaaatccag aaactcctca acaattcggt atcatgagca ttccaacatt aatgttgaaa
    17941 aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt caaaagaaca actacgtcaa
    18001 atgatcgatc aatatttata aaaacttaca gcaaaaataa gctgagggtg ggactgaatc
    18061 cagttccgtc ctcggttttt ttgcggttga gttaaggtta ttcaaattaa tattttttga
    18121 gtataattac gttgtcaggc tggattgttt ttttgacaga catgctataa taactttaat
    18181 tgaatatgaa ggggagtacc agatgaacga acgaatcaaa aacaaattag ctttacttcc
    18241 tgatcagccc ggctgttact tgatgaaaga taaaaatgga acgatcattt atgtcggcaa
    18301 agccaaaata ttaaaaaatc gtgtccgctc atattttact ggtagccata atacgaaaac
    18361 ggaacggctt gttagtgaaa tcgaagactt tgaatacatc gttacagaat cgaatatcga
    18421 agcattgctc ttagagatca atctgataaa gaaaaatgat cctaaataca acattatgtt
    18481 aaaggatgat aaaacctatc ctttccttaa gataacgaat gaaaaatatc cccgtttggt
    18541 gattactcgt aaggtactaa aagacaaagc actgtatttt ggaccatatc ccgatgtcgg
    18601 tgcggcgaat gagacaaaga agatcctcga tcgattattt cctctgagaa aatgcaaacc
    18661 tagtcagacg aaggagcctt gtctttacta tcatttggga caatgcctgt gtccttatta
    18721 tttcgacgta gatccgaaag tctatacgaa aatcgttgat gaagtgaaaa actttttaaa
    18781 cggaggacac gaaaaaatcg agtcagagat ccatcagaaa atggaaaaag ctgctgaaaa
    18841 catggagttt gaaaaagctg cagaataccg agaccagatc cgtgccattg agacaatcat
    18901 gacttgccaa aaaatgacaa atactgattt actggatcga gatgtatttg gttatgcgat
    18961 tgataaagga tggatgtgtg tccaagtgtt tttcgtaaga caaggaaagc taattgaacg
    19021 agatgtttca atgtttcctt tctataacga agcagaagaa gatttcttga cttacctcgg
    19081 tcagttttat caggaaaatg agcatttcat tcctaaagaa gtcttgattc ctgatacgat
    19141 cgacaagccc agtgtagaag ctttgttagc tacgaaagtg ctgcaaccaa aacgaggaga
    19201 aaagaaaaaa ttagttgaat tggcgagtaa gaatgcctca gttgctttga atgaacggtt
    19261 tgatctgatt gccagaaaac aggaacggac gatcggagca gttgaacgtt taggaaacgc
    19321 gatgaatatc ccgaccccaa tacgtatcga gtcttttgat aactctaata tcatggggac
    19381 gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa ccggcaagaa aagaatatcg
    19441 gaaatataaa ataaaaacag tcaacggacc ggatgattat gcatcaatga gagaagtcat
    19501 ctatcgcaga tattcgcgag tgattcgcga agggttgcct ttacctgatt tgatcttgat
    19561 cgatggagga aaaggacaag tagatgtggc aaaagatgtc ttagaaaatc aattaggaat
    19621 cgatattcct attgctggca tggtaaaaaa cgataaacat aaaacaaatg agttgctatt
    19681 cggtacagat ttggctgtag taccgcttga gcgaaattca ccagagtttt tcttactcca
    19741 gcgaatccaa gatgaagtcc atcgattcgc aatcactttc catcggcagc tacgcagtaa
    19801 aaacagtttt gcttcccgat tagatgaaat tgaagggtta ggaccaaaac gaaaaaaaat
    19861 gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca gccacgacag aagaattaca
    19921 agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt gataaattac atcaggaatg
    19981 aggaaagggc actttacaaa gaaagtaaag tgtcctatca ttttatgtac aacaaaaaga
    20041 gcgtttacgt tttgttggta aataataaat cgcttacgtc tggtgtctaa ggatatatgt
    20101 gatttcttga agaaggagtg aagaaatgtt agaagtcaag gatcttgtta aaacttttgg
    20161 gaactacaca gctgtcgatc atgtctcttt ccaaatcccc gatggaaaaa tcatggggct
    20221 aattggccaa aacggggctg ggaagacaac gacttttcgt ttgatcttgg attttttgac
    20281 acaagaccaa ggagaagtgt tatggaatgg ccatcatctt ggggagaaag attatgatgt
    20341 catcggttat cttccggagg aacggggact ttatccgaaa gtaacgattc aagaccaact
    20401 gctttatttt gcagaactta gagggaaatc aagaaaagag attgaaccaa aaatcgactt
    20461 ttggatggaa aaatttcagg taaaagggaa aaagacggat aaagtaaaat ctttgtctaa
    20521 aggaaatcag caaaaagtcc agctgatcgc cacgttaatc catgaaccta aattgatcat
    20581 tttggatgag ccattcagcg ggttagatcc ggtaaatgct gaattgctaa aagatggaat
    20641 attggaattg aaaaaacatg gttcatgtgt cattttttct agccacaata tggataatgt
    20701 agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga aaaatggttt tgaatggaaa
    20761 agtacatgag attcgtgaat catttggccg tacgaagtta ttcttggaat ctggtttaag
    20821 ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg atccttcatg aagacaatac
    20881 gttagagatt accttagaag atcctgaagc tgggaaagaa atctttactc gtgcgactca
    20941 gttcggctac attcctatgt ttaaccagca accgccaact ttggaagaga ttttcaaatt
    21001 gaaagcaggt gaaatcgatg cgtaagtttt ggattattac caaagatgtc tatttaaaaa
    21061 atgttaagtc gatttctttc ttgatcatga tattagtccc ttttatcatg ctgggtatca
    21121 tttatgtagc aggaaacttt gctcaacaaa atagtgatgt caataaaatc ggcgtattgg
    21181 ctgataatga gcaaatcagt cagcaaatga gccaagtcaa aacagatgat tttgagttca
    21241 aagcagttga ttctgaagaa aaagcacaaa aagagctttc ggaagaaaaa atcgatgcgt
    21301 atttagtagt ctcaactgag aatgataaga taaatgggat gctttatagc gaaaattcat
    21361 taggacaaag tacacaacta ctgatacaac aacaactgac tggcttgcaa tcgatgcttc
    21421 gagcgagtca attaggcatt tcaccagaag aagtagctag cttgagccaa caagcaagtt
    21481 tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat gacagtcgga gaagataact
    21541 cgacagtcca atacgtcgta agttatgtag caacgattgt cttgttcatt attattttga
    21601 cgtatgcgca gatcattgca caagagatcg cttctgaaaa aggcacgcgg atcatggaag
    21661 tgatcctgtc cagtacgact gcacaaaagc atttttacgg aaaattaaca ggtgttttac
    21721 ttgttgcatt aacgcaaatg gcattatacg gtattatttt tggtatagga tacagtcaat
    21781 tcaaagatat ggaaatggtc aaaaatatat tagacggtat ctcattggat aacttgattg
    21841 gaccattctt gtggtattct cttctattca tgatctttgg cattttgatt ttttctgttt
    21901 tagcagcttt atgtggctct ttggtcaaca aagcagaaga tacagcaaaa gcaattttgc
    21961 cagtgactta tctatctcta ggcggataca tgcttggatt gatactagga gcttcagatc
    22021 caaacaatat cgttattcgg atcacttcgt atattccatt cttatcttca tatattatgc
    22081 cgattcgctt agcaaatgag acagtcggta tgggtggagc attgatttcc ttgttggtac
    22141 ttgtagccgt aacattctta ttgatgatct tgtctgcaaa catgtacaaa tcgaatgtac
    22201 tcgtttatag cgaaggcgga ttatggagtt cattgaaaca atcgatttca attatgagga
    22261 atgaacgaaa aaaagcataa aacaatccta aaatacagaa ggaagctgga catctattgt
    22321 tcccagcttc tttttgtata gacctatttt atttatttta ctatattgga tatatattca
    22381 ttgaaaatag tgtataacaa ctttataata ttgagtatac caatttttgg aggcgtttta
    22441 atggaccgtt atttagattt actttcagaa aaattcccct caacggagga tgtcatcacg
    22501 gaaatcatca atctcgaagc aattttgcag cttccaaaag gaacagagtt atttttgagt
    22561 gatatacatg gcgaatttcc tgcttttgat catattctgc gtatcggttc aggaaatctt
    22621 aaagaaaaga tcaaagacct tttttctgaa caaatgacgg aaacggatat cagccaattc
    22681 actattttta cggcttatcc agaatatgca ttaacgactg attggtacaa agaacaagat
    22741 aaatcgcagc tgatccatca tttgatcgat cttttacggt ttacgacagt caaatatacc
    22801 cggtcaaaag ttcgaaaagg attgccgaaa gaatatagct atatcattga ggaactcttg
    22861 tatatcgatg accgtatcaa tggaaaaaaa gactatgtaa aaaaaatcat tgaacaactt
    22921 gttttgatga aggaagaaga aaaattttta acgaaacttg cacagacgat ccaaaaatct
    22981 gtcatcgatc atttgcatat cgttggagat atttttgacc gaggaactca agcagccaaa
    23041 gtgatggatc gaatcatgga tttttcttct gtagatatcc aatggggaaa tcatgatatt
    23101 ttgtggattg gtgcgtattg cggctctgag gcatgtttac tgacactttt acggattgca
    23161 gcacgctata attatttatt tgaattagag aaagaatatg ggttgaattt acgtccattg
    23221 ttttcttttg ctcatcaaac ttatcagaag aatccagtat ttgcgcctaa aaacagtaaa
    23281 aatctatccg aaagagagca agaagaatta gaaaaaatcc atcaggcact ttctatcctt
    23341 caattcaaat cagaacatca gttattggat cgtcgacctg aatttaaaat ggacgatcgt
    23401 aaattgctgg aaaaaatcga ctatgaagca tcaacgattg atttagaagg tcagctctat
    23461 gagttgaaag gaacttgttt tcaaacgatc cagccagatg atccaaaaaa attcttggaa
    23521 gaggaacaaa aagtgattga tagtttgatg tattcattcc agcactcttt gagaatgcaa
    23581 aaacatatga cgtttttgat cgaaaaaggg ggaatgtatc tcacgaacaa tcagcatgtg
    23641 ctgtttcatg gctgtatacc tgtagacgag aagggacagc tcttagcatt tgaattagac
    23701 caatcttatc gcggaaaaga actgctgggc ttttttgaaa aacagataac tgaaagcgca
    23761 aaagatctga ctgcaaaaga agatttagca accgatttga tttggtatgc gtggagtggg
    23821 ccattttcgc ctttattcgg caagagtaaa atggcgactt ttgaacgtta ttttctcaca
    23881 gagcctcata cgcacgtcga gaaaagcaat ccttattatc acttgcgaga tgaagagtgg
    23941 ttcagtgaaa aaattttagc agaattcgac acaaaagaag aagggtcagc aatcattaat
    24001 gggcacacgc cagtaaaagt aaaaaaaggg gagtcaccga tcaaagcaaa tggaaaagta
    24061 tttgtcattg acggaggttt atccaaagct tatcaaaaaa cgacaggaat tgccggttat
    24121 tcactattat gcaattctta tggtttccaa atcgtgacac attatccgtt tttgcctatt
    24181 gaacaacaat ttgcaaaaaa atccgaccaa acaaatgtga aaaaagtaat cgaacagcaa
    24241 ctaccacgca aactcaatcg tgatacaaca aaaggtatgg aactgcagca gcagattacc
    24301 cagctcaaaa ggctgttgga ctatcgaaag gacgattgag aagaattcca gtgaacattg
    24361 ttccatagac tggagactga gacaaaagtc gtgtcgcagt ctctttttcc ggataaacgg
    24421 tgttcttctg ccaactcctc ggtcacaagt cacactattc aacaaacatg aaaagctgtt
    24481 ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct cttggtataa
    24541 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtgaaaagc
    24601 cattttcaca aaatttttct taatactcga aactaaacag ctttttcaca accccctcag
    24661 aaagtctatt aaaattcatt cacaaagaaa tcgaataagc gctgttcaga tggatgtaca
    24721 cggtgggtaa gttccggatg ccattgaaca ccgaggattt tatgcatatc atctttgctt
    24781 tgaaccgctt caacgagtcc atcgttagaa aaagcgatgg cttttaataa aggtgaaaga
    24841 tctttgactg cctgatgatg gtaagaattc acaaaatatg gatcggggag aaggttgctt
    24901 aacaaactgt ctgaaacaat cttgacttcg tgagtcgtaa attgcggggc agtgggctgt
    24961 tgttcatgtt ttactgacca ctttggatat aatgaaagat cttgataaag tgtaccttcc
    25021 aaaacgacat tcaatagttg gagtccccgg cagactgcaa aaattggttt tctttgtttc
    25081 aatgcttcca agatcagtgc ctgttcaaat tggtctcgtt gaatatttgt ttccgttagc
    25141 tttggatgag gatcttccat gtaaagttgt ggactgacat cttgaccacc tgcaagaata
    25201 agtttgtcga tttgtccaat cagttgagga gctgaatctg gagacatgac tggcagaata
    25261 agaggcaatc cttgagcatc taaaactgca tccacgaatc cttgcggtgt atagctcact
    25321 tgatttcctt ggaaagtatc tgttgctcga atcagttgat ttccggcaat gccgatgatt
    25381 ggtttcattt gtattccccc taaagtagac ttattgtaac aaggatttaa aaaaaattaa
    25441 actatgtaag acttgcccct atacttatct gttcttgctg tgttactatc tgtaaagaga
    25501 aatttcgtgt gaaattcatg acgatagttg aaaatattgc ttaatttgtt ataataaaaa
    25561 gtaagcagaa ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc aaggggtgag
    25621 aaggacattt tattgaaggg gcctgcctat actaagttat ctttagaggt acatactttc
    25681 aatgaaaaaa gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag caagtgttag
    25741 aagctttgga acaaatccga aaagcagaag aagaaaatga aaagcgtgct caaacactgc
    25801 ggcagcagct gaagatctat gcaacagaaa aagagcagga attggaaacg atccaaacaa
    25861 actatcgaca acaactaaaa cagttggtgg aagaaaaaga acaatttgaa atacaagaat
    25921 tagaaaaaga aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca actttgaaag
    25981 atcaatatca aaagcatcag agtcaagcga tcggtgcgat cattgaaagg gtgaaagaaa
    26041 cttatggccg tcactaagat ggaaaaagtg accctcattt cagataaaag aaatcaggag
    26101 aggattttac aagcagtaca aggaatccaa aacgttgaaa tccgggatct gtatcaaaat
    26161 agaacaaaca accagtgggt ggagaagtat tttccagata ccacgattat tgataaagaa
    26221 gcaaaattaa gcgctttagg taatcggttg aaagacatcc aagaagctat ccaattcata
    26281 caacaccacg gcgatagccg tcaaaagaaa ctccatttga aacgtcaaga actttcctta
    26341 caagatttgg aagccaatta ttccgaagaa aatttcataa ataaattgaa agaagtcctt
    26401 gatttaaaag aaaagtggga acaactaact gaagaacgtg atcgtttaga agaagaggaa
    26461 gaatggcttc ttcattggca acagctggat attgctccaa gtatgtatgc aagtaagacg
    26521 accatttttg agatggggac tgtgagtgca gcaaattttg aaagtttcaa ggaagaactg
    26581 agtaagcttg atgacgttta tatggaagaa atagattata cgccaaaaca agtttatctt
    26641 tcgtatgttg ctttaaaaaa atcagttgct gttatagaag aaattgctgg gaaatacggg
    26701 ttcaataaag aaacgtatcc ttatgaacaa aatcctaaag aaaaactgca agagaccaag
    26761 cagcaattat ccattgttca cgagcagcaa aagaagataa cgacagcgtt aggtcagtgc
    26821 agtggctata tccaagaatt tgaatgggta gaagaagtca cacttgccgt tgcagaacga
    26881 gaaaaaataa aagaacggtt tatccatgcg acttatctga tcgttttgca aggttgggta
    26941 gatgcagaag aaaaacagaa tttgctgcat gtgttaaacg aaaccgtatc agaagaagat
    27001 atatacgtca gtttcgaaac accaaactca gaagagattc agcaggaagt ccctactaaa
    27061 ttgaaaaacc atccgcttgt cgaacctttt gaattgctga ctgaaatgta cagtctgcca
    27121 aaatatgaag aagtagatcc aacaccttgg atgacaccat tttatcttgt cttcttcggg
    27181 atgatggtcg cagatgttgg ttatggtttg ctgatgctca ttgggacaat actagctcaa
    27241 aaactacttg ttctaccacg cgggatgaaa cgtttcgtaa aattctttga gattctctcg
    27301 ataccatcga tcatctgggg attgatctac agttcatttt tcggacagtc attaccaaaa
    27361 gaactgtttg gcatccgctt gcctttccca atcctatcta cgacggatga cgtcaatacg
    27421 attttgattt tatccgtgat tttcggtctg atacagattt tggttggatt atttgtggca
    27481 gcaaaggaaa atatcaggag aaaagattac ttgagtgctg tcagtgatgg atttgcctgg
    27541 caagggatcc taattgggat cgttattgcg ttggttggtg ccatgttgat aaaaaataca
    27601 gcatttgttt accttggcgc tgggctatcg atcattgctg ccttatgtat cttgatcgtt
    27661 ccgattatcc aaacgccgtc taaagtcaaa ggtgctgcca aaggtgcgta taatctttac
    27721 ggactgaccg gctatatcgg tgatttagtc agttatacac gtttgatggc attgggtatt
    27781 tcaggcggaa gtattggagc ggcattcaat atgctagttg ctttcatgcc gccagctgct
    27841 cgattcagtg tagggatcct tttgatcatc gctttacacg cactgaatat gtttttgaca
    27901 ctgctaagtg cctatgtcca tggcgcacga ttgcagtatg tcgaattttt tgggaagttt
    27961 tatacaggag gcggacgtgc ctttgatccg ctgaaaacgg cggaaaaata cgtcaacatc
    28021 aatcacaaga aaaaaaacaa taaataattg gaggattttt atataatgat ggattactta
    28081 attagtcaaa atggtggaat ggtatttgcg gtattagcga tggcaacagc gacgattttt
    28141 tcagggatcg gttcagctaa aggtgttgga atgacaggtg aggcagcagc agcattgaca
    28201 acaagtcaac ccgaaaaatt cgggcaggct ctgattctgc aattgcttcc aggcacgcaa
    28261 ggtttgtatg gattcgttat tgcctttttg atttttatca acttaggaaa tgacatgtca
    28321 gttgtacaag gactgaactt tctaggtgct tctctgccga ttgcttttac aggcttgttt
    28381 tcaggtattg cccaaggaaa agtcgcagcg gcaggtattc aaattttagc gaaaaaacct
    28441 gaacatgcta caaaaggaat catttttgct gcgatggttg aaacatacgc gattttaggt
    28501 ttcgttattt ccttcctatt agtgttgaat gcctaagcat aaaggaggaa aagcagatga
    28561 acgccattga aaaaatcatc tctcagatga acgaagcagc agagcaggaa agagcggcgt
    28621 tagaacaaga agagcgaatg aagatcgatc aaaactttga acaaaaacgt acgcaagttg
    28681 aaacagaaca tcaaaaacaa aaagaaaaac aaatcgaatt actcgaaaaa aaataccgtc
    28741 agctaaggaa tcgccagcaa gtagaagtac gacaagaaaa tttgaatgcg aagcaggaat
    28801 ttttaagacg attgtttgca gatgcggtaa ctgaaatgga aaattgggat gaatctgaac
    28861 aaatccagtt tatcaaaaat gcattgtatt cattaccttt gacaggaaaa gtagcattca
    28921 ttgctggaga aaaatcagca gcctatcttt ctcagacgtt gttggatgaa tggaacaatg
    28981 aacttccctt tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg ggttttctga
    29041 taaacgatca aggagttcag tataattttc ttttcagcag ccttgtacaa gatatccaag
    29101 gaacgatgag ttttgagatt gccaatcaat tattcgaata aggagggatc agtgtatgga
    29161 ataccacgaa ctaaatccct tgattcgcgg aagagagctt gaacttttgt ccaatgagac
    29221 atttgaacgg atgattcaaa caaattctgt cgaagcactt ggtgaaatat tgaaatccac
    29281 gatttacagt ccatatattt atgacggata tgaatatgac ttcgaacaga atcttttaaa
    29341 ggagcagagc cgtctatttc gctggttaaa ggaatcagca ccggagcctg aaatcgtttg
    29401 gatctatacg atgcgttata ctttccataa tctgaaggtt ttgacaaaag ctgagatgac
    29461 aaatcaaaat ctggaccatt tgtatatcga cgatggattt tattcattag aaaccttgaa
    29521 ggatgccatt catacgcaag tttcaaatga acttcctgaa agcattatgg acagtatacg
    29581 agaagtacgt gattattttg aagagtctac cattcttcag ggaattgacg tgatttatga
    29641 tcgacagttt ttgacagagc agcgacggct tgccgataag ctaggatatc aagaattgct
    29701 gacagaagtc attgctttta ttgacttgac caatatcaca acgatggctc gaggaatatt
    29761 gcagaaaaga acatatgggt tcatgacgac ggtactttcc agctcgggaa gtatcgaaaa
    29821 agaagtattt ttgaactttg tagataaaga tatggaagat tacacgaatt ttctattaga
    29881 gacagattat gctgattttc tgcgccctgt catccgtgag ggaaatattg atctcgtaaa
    29941 gcttgaacag ttgaaagatg attatctaag ttcattgtat caagaagcac aaacccaggc
    30001 tttcggacca ctgccactac tagccttttt gaacgcaaaa gaggtagaaa gcaaaaacct
    30061 tcgtttactg gttatcggca aaagaagcca tttcgaatca gaaacaatca gaaagcgggt
    30121 gagacaagtc tatgacgcat aagatcggtg tagttggcga caaagattct gttttgccat
    30181 tcaaattgtt tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt cgtcaggcaa
    30241 ttgaagaaat ggcaaagaat gagtatggcg tgatttatgt cacagagcaa tgtgcagcat
    30301 tgagcccaga aacgatcgaa cgttacaagg aacaaatgat acctgctatc gtattgattc
    30361 ccaatcatca aggatcatta gggatagggc tggaagaaat tcaaaaaaac gtagaaaaag
    30421 cagtaggtca aaatatctta tagtatgaaa aggagataga aattttgcaa attggaaaaa
    30481 tcataaaagt ctccggacct cttgtaatgg ctgagaacat gtctgatgcc agtatccaag
    30541 acatgtgtct agtgggagat ctaggagtta tcggagaaat tatcgagatg cgtggcgatg
    30601 tggcgtctat tcaagtatat gaagagactt ctggaattgg tccaggcgaa ccggttcgct
    30661 ctactggtga agctttatct gttgaattag gaccagggat catttcacaa atgtttgacg
    30721 gaatccaacg gccattagat acctttatgg aaatcacaca aagtaatttc ttagggcgcg
    30781 gagttcaatt gcccgcattg gaccatgaga aaaaatggtg gttcgaacca acagtagaag
    30841 ccggagaaac ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca aaagttataa
    30901 aacataaaat catggtgcca aatgggataa aaggaacgat caaaacaatc gaaaaaggtt
    30961 cattcactat tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac aaaaatttga
    31021 cgatgcttca aaaatggcca gtaagacgta gtcggccaat caaagaaaaa ttaaacccgg
    31081 atgcacccat gatcactgga cagcgagtga ttgatacatt cttcccagtg acaaaaggag
    31141 gagcagcagc tgtacctggt ccttttggtg cagggaaaac agtcgttcag catcaaattg
    31201 caaaatgggc cgatgtggac atggttgtct atgtgggatg tggcgagcgt ggaaatgaga
    31261 tgacggatgt tttaaatgaa ttccctgaat tgattgaccc taatacagga gaatcgttga
    31321 tggaacgtac ggtattgatt gctaatacat caaacatgcc agtagcagct cgagaagctt
    31381 ctatctatac gggaatcaca attgcggaat atttccgtga catgggttat gacgttgcta
    31441 tcatggcaga ctccacttca cgatgggcag aagcacttcg tgagatgagc ggacgtttgg
    31501 aagaaatgcc tggtgatgaa ggttatccag cttatcttgg ttcacggctt gcggaatact
    31561 atgaacgatc tggaagagtc attgcattag gatcagaagg tcgcgaagga agtataaccg
    31621 caatcagtgc tgtatccccg tctggagggg atatttcgga accagtcaca caaaatacat
    31681 tgcgggtcgt aaaagtattc tggggtcttg attccagttt agcccaaaaa agacattttc
    31741 cttcgatcga ttggatacaa agctattctc tttattcaac agaagtaggg aagtatatgg
    31801 atcatatcct tcaagctgac tgggcatcca tggtcaccga gggcatgcgt attttgcagg
    31861 aagaacagca gctgaatgaa atcgtgcgtt tggtagggat tgattccctt tcagataatg
    31921 accgtctgac attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag caaaatgctt
    31981 ttgatgatgt cgatacattc acttcaagag agaaacaatt caaaatgttg aagatgatct
    32041 tgacctttgg tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta agtgagatca
    32101 tggacggtac cgtatcgatt cgtgaaagga tcggacgaag caaatatatc ccggaaacag
    32161 aattagaaaa actagatcaa attattggtg aaatcaagca aacgatgcaa acgatcgttt
    32221 cagaaggagg catgagcaat gattaaagaa tatcgtacga tcggtgaagt cgtcggtcca
    32281 ttgatggcgg ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga agtccgtatg
    32341 caaaatggcg agattcgtcg tggacaagta ttggaagtcc aagaagataa agcaatggtc
    32401 caaatattcg aaggaacaag tggtatcaat ttacgtgact cttctgtacg atttttaggc
    32461 catcctttag aactaggtgt atcggaagac atgatcggtc gagtattcga tggtttggga
    32521 cgaccgaaag ataacggacc agagatcctg cctgaaaagc atttggatat taacggagaa
    32581 gtaatcaatc cagtagctcg ggattatcca gatgagttta tccaaacagg tatctctgca
    32641 atcgatcatt tgaatacatt agttcgtggg caaaaattac cagtattttc tggctctggt
    32701 cttcctcata aagaattagc tgctcagatc gcgcgacaag caacggtact cgattcatca
    32761 gatgatttcg ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc tgaatttttc
    32821 atggaagatt ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat gaatttagca
    32881 aacgaccctg caatcgaacg tatcgctact cctcgtatgg ctttgactgc tgcagaatat
    32941 cttgcttatg aaaagggaat gcatgttttg gttatcatga cagacatgac caactatgca
    33001 gaggcattgc gagagatttc tgcagcacgg cgagaagttc caggaagaag aggatatcct
    33061 gggtatcttt acacgaactt agctacttta ttcgaacgag caggaagaat ccgcggacta
    33121 aaaggatcgg ttactcaaat tccgattcta acaatgccag aagacgataa aactcatcca
    33181 atccctgact tgactggtta tattacagaa ggacagatca tcttgactag agagctttac
    33241 aagagtggga ttcagccacc aattgatgta ctcccgtctc tttcgcggtt aaaagataag
    33301 ggaactggac ccggaaagac aagagaagac catgcagcta cgatgaatca gctttttgca
    33361 gcctatgcac aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga atctgcttta
    33421 tctgatgtag acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga atatgtcaat
    33481 caaggattct acacgaaccg ttcgattact gaaacacttg atctagggtg ggaactgcta
    33541 tcgatgttgc caagaaccga attaaaacga atcaaagacg acatgctgga caaatatctg
    33601 ccagaaggga agtgagttct gatggcacga ttgaatgtga atccgacccg catggaattg
    33661 actcgattga aaaaacagtt gacaactgcc acaagagggc ataaattatt aaaagataag
    33721 caagatgaac tgatgcgtca attcatcctg ttgattcgaa agaacaacca gctgcgtcaa
    33781 gaaatggaag agtctatgac agaagcaatg tctgattttg tattggcaaa tgcttcgatc
    33841 acggaacctt tcatcgaaga attatttgtt ttgccggcgg aaaacgttga tttgtctatc
    33901 gtagacaaaa atatcatgag tgtaaaagta cctgtgatga attttgaata tgatgaacag
    33961 ctgacagaca cacctttgga atacgggtat ttgaattcca gtgccgaact ggatcggtca
    34021 atcgatcggt ttactaccct gcttccaaaa cttttggaac tgacagaaat cgaaaaaact
    34081 tgtcaactga tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc attagaatat
    34141 atgacgatcc ctcaattaga agaaacgatc tattatattc gtatgaagct ggaagaaaac
    34201 gagcgcgcag aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac tgactaagtg
    34261 gtatcttttt ttaaaagagg aaaacgatga ttaaacaaag caaacaaata aagaaacgtt
    34321 tgccggctgt tcagcttctc gcattaggat tttttctcct gattttagtt ggcggcggcc
    34381 tacttaccct accatttttt agtaatagtg ggcatgggac aaattttatc gatgcgcttt
    34441 tcactgcaac ttctgctgtc tgtgtaacgg gattgacaac attgaataca gcagagcatt
    34501 ggaacagtgc tggtcaattt ttgatcatgc ttttgattga gatcggcggt cttggtttta
    34561 tgatggttcc gattctatgc tttgcattga taaagaagaa agtcagtctc agcacacgta
    34621 ttatattaaa agaagcgctg aatctagaag aagtatcagg tgtgatgaag ctcatgcttt
    34681 acatcctaaa attggccttt gtgatccaat tgattggtac ggttgcttta tcttttgtat
    34741 ttgtgcctga atttggctgg ttgaaaggta tctggtacag tttgttccat gcaatctcta
    34801 gtttttgtaa tgcagggttt gatttgttag gagacagttt agccaatcac cagcaaaatg
    34861 tttatttgat catggtggta tctgctttga tcatttctgg tgggttaggc ttcatcgtat
    34921 ggcgtgattt actggaatat catcagaaaa agaagatgac gattcattca aaaatcgcat
    34981 taaccgttac aggtaccctt ttattaggcg gattcgttgt gttctttttc acagagcgca
    35041 atggggcaac gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc ttttttatga
    35101 gtgtgacacc tagaacggct ggatattatt cagttgatta cctccatatg agccatgcag
    35161 gtctgatttt aacgatgttt ttgatgtaca tcggcggaac atctgggtcg acggccggcg
    35221 gattgaaaac aactacgtta ggtgtgttgc tgatccagat gcgttcgatg ttgaaaggac
    35281 gtacgagagc ggaagcattt ggccgtacca tccgacaagg cgcagtgttg cgggcactta
    35341 ctttattttt tgttacgttg acgttgtgtg tagtcgccat catgatttta tcggtgactg
    35401 aaacgatacc taaaacttct ggcatcgaat atattgcttt tgaggtcttc tctgcgtttg
    35461 gtacagtagg tcttacgatg ggattgacgc cagacttgac tgcaataggg aaattgatca
    35521 ttatagcgct tatgtacatt ggacgagttg gtgtattgac tgtggtcttc tctctgatga
    35581 caaaagcaca aagaacacaa tcaaaataca aatatccaga agaaagtgtc atgattggat
    35641 aatttcatct tctgacagaa acctagtgat ttagtaaaat aagaaaataa aataattttt
    35701 cttgcttctt ggatacttta agtgcaaaat gttcgtttat tttttgaggc actacccgtt
    35761 gttgaacctc gggtagtgcc tcttttttat tgaaaggaat caataagatt tgattccttt
    35821 atttatcaat aggttataat tcatattttt ttattttttc ttttacgata cttagtccag
    35881 aagctaaaat ttcaaggtta ttagaggata gggaaattcg aacgaatgca tctggttgcg
    35941 cacgtgtagt aaagcgtctg gaatgataaa gtcggatacc gttagcaaga aagtcattct
    36001 caattatctt agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa gggacacgat
    36061 tcgttggttt ttctactttg aaaagagaat caaaaagctg attggctctt tttgtaagac
    36121 ttaatttagt ttctaagatc ctatttgctt catttgaatg gaggatttga gtgatgactt
    36181 ccacatctaa tccagaagtt ttcacattta tattaaaaat tgcttgttct atcttgcttt
    36241 ggacatgtac tggaactaca agataagcaa ctcgcaatcc agaacaaata aattttgtca
    36301 ttccagctaa gtagattgtt tggttaggta ataaattttg gaaaggagga atgatcgtat
    36361 gttgatagta agttgttaaa aatgaatgga tatcatcttc aataacccat agaccttctt
    36421 tttcaatcac ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac cctataggat
    36481 tattacaaga aggcataaga aagacaccat gaatcttctt ttttctacat tcctgaatca
    36541 atatatcagg tcgcattcct tgctcgtcaa aatcaattgg aacgatctct aaatgatata
    36601 actgagccaa ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt ctatcacctg
    36661 gagagaaaag tgcagaaagt gtgatagcca atccattttg aacaccggaa acaatagata
    36721 gagattctaa ggacggaatg actccttgag tttttaacca atttgctgca gtagtcaatt
    36781 gaaaatttgt gcctttggga tcttggtaat taagcaaacc aacaagattt gggttattac
    36841 tagttgattg aataaacggg acaacgaatt tattacattc ttcaaaagaa ctaaccagac
    36901 caaaatcaat gatatgttcg gttacttcat tggtagagat ggtgatggac tcaaagacat
    36961 tgggagaaat aaatgtgcca cttccaatat tcgtatacag caaacctttc tcaattccat
    37021 atttataagc cttgccaacg gtagtaaaat ttaaatctaa ataatcagct agctctcttt
    37081 gggagggtaa ctgagtgttt ttctgtaatt tattctttaa aatatcttgt tctaattgtt
    37141 gaatcaacga tcgataataa ggacgcttca atttttgttt gtctggtcgc caagttaacg
    37201 ggtaattgtc ataagaatta ataggcattt tctccactcc ttaaaattgt ataccataca
    37261 attttaccat tgattgtatt gattttagaa gttaatcttt tgtgaggagg gaattgtcta
    37321 tgaaaattat taattttgag gaagtagtag cttcttttag ttttgaagaa gcgattcaag
    37381 taatgaaaaa atgttttttt gattatgaaa gtggtaaaat cagccaaaat cctcgattgg
    37441 tggaaatttt acctgacgga aaaggcaaaa atatgtttgc gatgatgcct gcttatttgg
    37501 gggagaacag atattttggc acaaaagtca ttaccgcttt tccggataat catcagtatc
    37561 atttaccttc tcatttagga gagattttct tgtttgatgc tcaaaacgga ctacccaaag
    37621 cattgataaa cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct gcgctggcaa
    37681 cagattactt agcaaatccg catgcagaaa cacttgcttt aattggagct ggtcaacaag
    37741 cattgtcgca tttgactgcg atttctacca ttcgtcccat cactacagtg tttgtttacg
    37801 atattttaga agaaagacga aatactttta tcgctaaagc taaaaaacag tatcctaaaa
    37861 taacgtttat aaatgcaata tcagtagaag atgcagtacg tccagcggaa atcatttgta
    37921 cgttgacctc tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa gacgcacata
    37981 tcaatgctgt gggcacattt actcctgata caagagagat aagtacatcg ttgatggaaa
    38041 cttctcaaat ctatgtagat gattactcag ctgccttaac agaaagcggg gacctgctta
    38101 tagccatggc agaaggagat ttagaggaat cttgtattgt tggtacatta ggcgaattag
    38161 tttctgagaa aaaaacattt tataaaggag aatcgaggcc gacgattttc gacgcagttg
    38221 gtctagctat tgaagatctt tgttgtgcag aatatattta taacaaatta gagaaagggg
    38281 ctttaaaatg aacttagatt attacgtagt tgatgcattt gcagatgagg tgttcaaagg
    38341 aaatccagca gcggtttatg tgctagaaga atggctgcca gaaggtacga tgcaaaaaat
    38401 tgccattgaa aacaatttat ctgaaacagc atttacagtg aagaaaaacc aagaatttga
    38461 gcttagatgg tttacgccag atcgagaaat cgatctttgt ggtcacgcga cattagcaac
    38521 agcatttgtt ttatttaact attacaaaat acctgatgaa acgattaaat tttctactca
    38581 aagcggtaat ctatttgtga caagaattaa agattattat tacatggatt ttcctagtat
    38641 tatgccgaaa aaagtgccga tattgtctga atatgaagaa gcgattgggg ctaagattaa
    38701 agaagcctac ttagcacgtg atttattttt cgtattagaa gatgaaaaaa cggtggctaa
    38761 attgcagcca gattttacag ctatcaaaaa ttttgaatta ggaataggag tcatcgtgac
    38821 agcagaaagt gtgcaaaaag actttgtttc tagaaccttc tttccaaaat taacaatcaa
    38881 tgaagatcca gtatgtggat cagcacattc taatcttatt ccttattggg cagaaagaat
    38941 gaataaaaca aaattgtcag cttatcaaat gtcatcaagg ggcggggatc tagaatgtga
    39001 attaaaagaa gatcgtgtga taataggagg aaaagcagtt ttgttttcta aaggtacctc
    39061 gtttatttga tttctttcaa atgaattaaa aataaattgg tggagagtgg caaccttata
    39121 gctacaatgg ttgttaaaaa aagacgcaaa ctttttcaat ttaatgaaaa gtttgcgtct
    39181 cattttaatt attggtgttt gttttttatt caatcaagag taaaatgccc catactaact
    39241 atcctcggat gacttcgatt ttataaccat cagggtcaat gacaaagtag taagaaggcg
    39301 cagtgccagg taatcctttt aagtcagtaa tctcaagacc agcagcttta tgttgctcgt
    39361 gtaatttttc tagatcatca gctgctatag cgatatgacc atagccgtct cctagatcat
    39421 acgcaccatg atcatagttg taagtcaact ctaattcata gtcatcccca ggaagtgtca
    39481 aataaacaag tgtaaatttg ttttctggaa aatcacggcg gcggctttct tcaaaaccaa
    39541 atgccttttg ataaaattcg attgatgctt caagatcttt cactcgaaca catgtgtgag
    39601 ccattttcat agattcatct ctccttttcc ttttactcaa tcataacaaa aagaagtaag
    39661 gaaatagttg atttgtgctt gttcatgaga aatttctcat gtaaaatgga tagaaaataa
    39721 aactgttaag gtggaaacta ttcatggata aaccaatttt tggtgaaaaa aagcaatttg
    39781 ctgattatca aacccgttat gcagcatata ttgtcataga aagagatcag cagattgcag
    39841 taatcgaggc tccaaatggt gctttttttc ttcctggcgg ggaaattgaa ggagaagaga
    39901 cgaaagaaga agcgattgtc cgtgagctga tagaagaaat gggaattgtg gcagaaattt
    39961 cttcttatct gggagaagct gatgagtatt tttactcgaa ttatcgtgca acttattatt
    40021 ataatccagg atatttcttt gttgctgatt cttggaagcg tataggagaa ccaactgaaa
    40081 agaccaataa aatttggtgg gtgagccctc aagaagcgct tgtgaaattg aaacgtggta
    40141 gccaccgatg ggcagtcgag agatggatcg aaaaaaataa aaactgaaag aaagcgagta
    40201 gatcagcttt ctctcagttt ttttatttat tacagtacat attttttgat tgcttcagct
    40261 acaccgtgct cgtcgtttga tgaagtgatg acatttgctg cttctttgac aagagaaacg
    40321 gcatttccca ttgctactcc tagaccagca tattcaatca tggataagtc gttctcgtta
    40381 tctccgatag ccatgatttc atcttgatca ataccaaggt gctgtgcgag attcgcaact
    40441 gccgcacctt tacttgcttc tttatttagt acttcgaagt aatagggcgc acttttgacc
    40501 gttgtgtatt tatcacggaa agtttgcggt aaacgcgcaa ttgctgcatc taaaatttct
    40561 ggttcgtcga tcatcatcat tttgacgatg ttcatgtctg gtgtcatttc ttcaggtgtt
    40621 cgatatttta gcggcatctt gactaaactt gcttcgtgga tcgtataagc gctgatatct
    40681 cgattaggcg tgtagatcgc tttgtcatcg atcgaatgga gatgactgcc gacacggcgt
    40741 gccattactt cgatctctag gaaatcttca tgtgtcagtc cgtgacgggc gatgatttta
    40801 cctgatttcg tttgttgtac gagtgccccg ttataagtaa taacgtaatc attatcttga
    40861 aaaaggttca actcgtttaa ctgttcctgg acacctggta agggacggcc agtgcataaa
    40921 acgatcgaaa caccttttgc aacagcagcg gcaatcgctt cttttacttc ttttgttact
    40981 tctcttttct catttaataa agtaccatcg atgtcgatag cgactagttt gatagacata
    41041 atttcctcca atatatttta atttatttca atcaatgcgc cattacggat atggctggca
    41101 aattcttgat aagtttcgtc aaataaatca taatgatcac gcaaggacgg atcaaccatt
    41161 tcttttggaa agaaaaatcg ctcatctcct tgttcttgtc ccgctaatgc agcaacaagc
    41221 tgactaacct gtgataattc tatcaaagtt ccgtcatttc gcaaaatctc gatttgtgtg
    41281 cgatgtctgc cttgttctgg gcgataaaaa tcataaggta gatcgtagct agaattcaca
    41341 gcagtgtaat aaacaggatt atatccgacc ttttctacta acaaagtgag ttcttggact
    41401 aattttgaat cccggttgcc agaaaaagta gcagatttca gtggtttacg attcaaaaaa
    41461 cgtttagcta aatcatttaa aataggatca gcagattcag accattgagt aaagtaagtg
    41521 cccaagaccc catcatcaag tttgaggtat tcttctaaag taaagcttcc gtcaagaaat
    41581 ggcagaagta attgagaatg gagttcaaaa ctgctggcat cttcttgata caactcgtgt
    41641 gcgcgatgaa gcagatgctc caaaattact tccataccac gggaaacagg atggaagtac
    41701 acttgcacat acatttgata tcggctaacg atataatctt ccactgcatg catgccactc
    41761 atggaaaaag cgatcccgcc ttcataagga cgaatcacac gtaatattcg ggtaagatca
    41821 aatgttccat attctgttcc agtgaaataa gcatcccgaa gcaaatagtc cattcgatct
    41881 gcatcgatct gactagaaat catttggaca acttgtgggt tggggtaagt tttttggatc
    41941 acactagcta ccttttcagg aaaaccttct tctactcggt taagaatctg atacacttct
    42001 gtttctggag aagtgatgat ctctacagtg atggcttcat gattcgtatg gaagatatgt
    42061 tcaaatgtat gagagtaagg accatgaccg acatcatgca acagtgccgc acataaagtc
    42121 accagccgtt cctgatcatc ccagcctccg ttgcctattt tttcttttga aaaatttcga
    42181 gaaaagatat cgcagattcg tcttgaaatc tcataaacgc ctaatgaatg agtaaaacgg
    42241 ctatgttctg ctccgtgaaa agtgaaagaa gaagtgccta attgtttaat gcgtcgtaga
    42301 cgttggactt ctttcgaatt gatcaaatct aagattactt gatgctggac atggatatag
    42361 ttatggactg gatcacgaaa aactttttcc ataggcagca tttgatattt ataaggggtt
    42421 gtcataatgt agctccttta tgttattacg ttgttccatt ctctggattt ggttgttcca
    42481 ttgctccagc tggatttctt tttttagcca gtcggtagaa tcctgtaaat cacttgcaga
    42541 atacagcttt tcaaaagctt ttgcaaatgc ttctttgact tcttttacaa aaaggggttg
    42601 gtcaagcagt tcttcaagtg ttgccattgt agcaggatcc actgcaggat agccatttgt
    42661 accaaagtct tcttttaaag cagattgata aaatcgcttc atgacttgcc cacgtttttc
    42721 ctgattaccg ttgacactta agtacatcat aacagaaatc ccatttttga tccgacgctg
    42781 agcgataccg gcaaattttt tgccatggat gcttaaatca taagttccag gacaataaga
    42841 atgggtgatt tcgaatgctt gtatcggtgc ctttggaaaa gcgtatttag tcaataggag
    42901 catttgctcg taagcgtcat ctatggttat tttttttgtg tcactttggg ggaaaatcaa
    42961 agaaatattc aaaattcctt catctgatac gatccctaat ccacctgaat ttctgatcac
    43021 tggagcgtaa cctgcatctt ccagtgtttg gatcccgtca cttagaaaag gagttcgaac
    43081 atccttcata cctaagatga ctgtgcagtc atattgccag aaatgtaaaa agatttgctg
    43141 gtgttcttta gaatattccg ttaatatatc tgttaatgca aaaggggaaa agtcgttttt
    43201 ttgataaatg tgctgatcta gtcgaatcgc agggttcatt caaccggctc ctttgtataa
    43261 attcatattt tctacaatat tatactctaa aatcaaagag ttgttttgga aaaaaggttt
    43321 tctgtaaaaa tggacaagtt ggttgacaaa acaaaagaaa ctaggaacaa tgtagctatg
    43381 agaagtaaag gagaatgaat aatggaatta tcacaaggaa caccgatttc aatcaaattg
    43441 cgtacaaaag taaaacagca aggggaagtg caagattttt attttgattt gaaagggcaa
    43501 atggtgacaa ttggcgatac gctgtacata cgttataaag agattcagga ggagacgggc
    43561 gatgagattc ctgtaacgat caaattagtt ccagatggtc atgtacagct gatccgtgca
    43621 ggagaaatgc ggatgcgttt gaaatttggc tataaagaac ggttagaaac atcctatcga
    43681 acaccatacg gtatgatcca gattgctact tttacgaaag aactgcatgt tagtttgaaa
    43741 gaccgtccca cagcagggaa ggttcgaatc gattatgatc tgttcatggg accggaaaaa
    43801 atcggcgaat attatttgac cttggatttt acagcgtaaa tataaaagaa aagtttgatt
    43861 ttttgtgtct gattttgtat gataaggagt agactgtgaa aggacgtgtc ctgattggaa
    43921 attagtgtat ttgaaggtgc aaacaaaagc gaattatcaa tgatcgaagt agcacacgcg
    43981 atcttagaac aacgtggcga tgtcatggat ttttctgatt tagctaacca gatccaaaac
    44041 tatcttggga aatcagacag tgaaattcgt gattcactcg cacaatttta tactgacttg
    44101 aatattgacg gcagctttat ctcattaggg gataatcgtt ggggcttgcg ttcatggtat
    44161 gctatcgatt cgatcgatga agaagtaaac catggaatgg acgaagagga tgaagatact
    44221 ccacgtcgcc gtaaacgcaa gaaagtcaac gcctttatca atgatgatga agacgcgatc
    44281 gattacaatg acgatgaccc agaagataca gatctgacag aagaagatga cgatgatctg
    44341 tttgatgacg atgatgatga agatgaagaa atcgctgctt ataattctga cttgcaagaa
    44401 atcggagcag atgacccgac agatgatgaa gaagacttgc caggaaatat cgaagaagat
    44461 ttaagtatca tagacgaaga cgatgatgat gaagattatg aagaggaaga attttctgac
    44521 tctgaaaaag aataaacatt attttcttac gagaggttgt gacagagacg atcagcctat
    44581 cagaataagg aggtgtgaca cgatttttgt cacaccttct tttttgtata taaaaatccc
    44641 aagtaaatac ataaaaaata aaaaaattga aacttttagc gcaccttgta cgtcatatta
    44701 agtgaggcag aaaggagtgg agaatgattt ttactggata taagaaaaaa cggcagatcg
    44761 aaaaataccg tcacctgctg aatggactca tcgaaactga ttatcaaaaa atgttccgta
    44821 tcacgatgaa ttacgtacat gataaagaag aggccttaga tgtgatgcaa gatagttttc
    44881 ataaagccct gaactatttt gaaaaagaaa aagagatcga gcattttagt gcatggtttt
    44941 atcggatatt gatccgcacg gcattagata gttggcgcag acaaaaaagg acgcctgtgg
    45001 acttatttga aattcaagat atcccccaac agttaccttt agaaacttcc gttgcagagc
    45061 tccatatgat tttagataaa atagacagtc cggaaaaaga gattctgatc ctctactttt
    45121 ttgaaggatt tcgtttgaaa gaaattgcga ctatcttgga tatgaatgaa aacacagtta
    45181 aaacgaaaat gtaccgctca ttgaatgctt tacgtcaaat cctagaatga aggagagaag
    45241 agtgccatgt attcccaaaa agaaaattta aaaaaactaa agaaaactta taaagaacaa
    45301 gcaattccca aagggcttaa agaggagatc cgccagcgtt ttattatcga agagcaatca
    45361 tttataaaag caaagcggag aaagagacgt ctccaagggt tcggagtatc tttggctatc
    45421 gtaatgatta caacaagtag ccttcttttt aatagtcaag ttcgaagctt tgcagaagat
    45481 ttacccatct taggatcagt cattgaattg atcttaggcg aacgctttac tgatcgatct
    45541 gaaaaaatcg atattcaggt accgaagatc aatacacaaa atgagaaaga aaataaaaca
    45601 attcatggat taaatcaaaa atactttcgc gaaggacaag cagattttga aaaagctgaa
    45661 aaagaatatg gaaattttga aacggatcat taccaagttt tgggagatta tcagaaaatc
    45721 gtagacgata accggtttct cgtgattgaa aggcagatca cccaaacagc agcagatagc
    45781 catgttgaaa aaagatacga cacaatcgac aagaaaaaca gtgtccagct ctcacttcca
    45841 cttttattca aagatgacac ctatctatct gttttgacaa aagaagtcaa acggcagatg
    45901 gccgaacaag tcaaggaaga tccatctaag tactattgga ctgagcaaga tattcaagaa
    45961 gggacgatag aaaaaccaac acttgttacg cctacacgaa gtttttattt gaacaaagag
    46021 catcaattag ttttgacttt ttctcaatac gaaattgctc ctggttatat gggtacacca
    46081 gaatttgtca ttcctaagtc cgtgacaaaa acgattttgg catcggaaga ttatttggat
    46141 cattagacga gaagtagttg cgactagcag tttcaatttt caagtaagta caagtaaata
    46201 ataagaagag aaaaagagaa agaagagata tagtgaaaag aaattattta gttatcagta
    46261 ttattgcttt atccgtgctt ttgtttttcg aaattacttc ggtggcctcc ttgatcaccg
    46321 aagtaaaagg acaaacacac tcttcggagc attcgtcaca atctttcaaa caatcagaac
    46381 aatcaaccat taaaagccaa tcaactgaaa cagctaacgt caaagaagcc attcaagaat
    46441 cagcggaaaa ggtacaaagt cagtcatcac aatcaacaca agacagtaca aaaacgagcc
    46501 gagatccggc tgtcaaaagc gtcgcaatca gctttgatga tggaccagga gcgaccacaa
    46561 caccgcagct tctgcgaatc ttaaaagaaa aaaacgtgca tgccactttt ttcgtcttag
    46621 gagaaaatac ggcgcagcat cctgaaatcg tgaagcagac agctgaagca gggcacgaga
    46681 ttggcaatca cacatacgat catcagaatc tggccactct ttctgcccag tcgatgacgg
    46741 aggaagtgac gaaagcagac actgagatca aaaaagtcac tggcaaaacc cccacttttg
    46801 ttcgtccgcc ctatggttct gtcacgaatg taggagcaac gatcatccag cgaccgatca
    46861 tcgaatggtc tgttgattca gaagattgga agacaagaaa tcctgatttg attttgcaga
    46921 agatccaagc aacagtatac gatggggcaa ttattttatt ccatgatatc tatcctgaaa
    46981 cgattcgtgc cgttcctcaa gtgatcgatt atctgaaaga gcaagggtat cgcatcacga
    47041 cagttgggga tttattaggg catccaacgg ctgtcgagaa ctattatggc agaaacgatc
    47101 atcggccagt tcagtaagtg agacaagtat ctttaataaa taacacattt taataagtag
    47161 ttgaacacat aactacctta cttgcaaagg cttaacaata tctatacagg tacaacaaaa
    47221 aactgtccac caattttctt tggcggacag ttttcactta aaaataaaga agcaactatt
    47281 cgtaaattat gctaaaatag cagtagaacg cgtcctgagg gaatcgaacc cccgtctcaa
    47341 gaaccggaat cttacgtgat atccactaca ctaaggacgc aagcactggg ataattgtaa
    47401 ctaaacagat aaaaaattac aaggggattg gacaaaaaaa tgaaatttga gcaaaatttt
    47461 tcacaaaaac agcagcagac acagaaacta gccatgacgc aacagttgca acagtccatc
    47521 caagttttac aatataacag tgaggagctg ttggcattcg ttgagaatca agcaatggaa
    47581 aatccattgg tggaagttgt tgagccggaa tggcagccag attacataaa agcttcttca
    47641 tcttcttatg aaggagaaga gacaaattac ctaaatcaga ttcctgatac aaaagggtct
    47701 ttatttgatt cattgatcga acaagtccat ttgaattacc gagatacttt tttgaggaaa
    47761 atcgttcttt atcttgtaga atacatcgat ttgaacggat ttttgacgat tagtttagaa
    47821 gaagccggaa aagagattgg agctacgcct atccaaatgc tcgatgcgtt gactctgatt
    47881 cagcaattag aaccagctgg agtaggtgca cgtagtttgc aagaatgtct catgcttcaa
    47941 acagaacgag atgattatgc gccggaatta gcttatatcg tgttggaaga atgttttgag
    48001 gaactggtgg agcgaaaatg gaaagaaatc gcacaacgtt tttctgttga tctacatgct
    48061 gttcagcaga tattcgacta tttacaaacg ttgagtccaa gccctggccg gattttcgac
    48121 cgtagcagcg aattgtacat tattcctgat gtccgggtat tagtggacga cgataaaaat
    48181 gtacaagtta tctcaaaccg aaaaaatcag ccaaatatac gctttcaaga gagttacttc
    48241 aaacaaatgt cacaacaagc agataaagaa actgaaaatt atttaaaaga acggaaacag
    48301 gaattcgaat ggttgaagaa aacgatccta caacgtgggg atacaatttt gcgggttgct
    48361 caagtcattg tttcccggca aaaagctttc tttatcgaca aggaacgtcc aatcaagcct
    48421 cttacgttaa aggaagttgc aactgagata gatgtgcatg aatccacggt cagtcgtgca
    48481 gtcaacggga aatatttgga aacagacttc ggtgtatttg aattaaaaaa atttttcact
    48541 acacgaattc ctacgaatag tacagaacag acagaagacc tttcagcaga tacagcgaag
    48601 aaaaagctgc aggaattagt cgatcaagaa gacaaaaaca agccgttgtc tgaccagaaa
    48661 ttggttgaat tgctgaaaaa agatgagata gcgatctcaa gacgaacagt agcaaaatat
    48721 cgtgatttat taggaatccc gagttcatca aaaagaaaac gttatgataa ctaatcagtc
    48781 aaaagactag aaaaagtatt tcactttttc tagtcttttt agttgaaaaa cgagagcaaa
    48841 actgttagac ttacagtcgt gaggttgaga tttcccttta ttgatgtgaa attcgaatag
    48901 ttaatttctt tttttatcgt atatgggtcg ttttaagtct acattggacg taaagtgtcc
    48961 aactaaggag atcgctatgc tggatgaaat aaaaatgatt gaatctgttg caccagatat
    49021 tatcgaagtg ttgcaagaac gctataaaat tttacgaaat atctattgga tgcagccgat
    49081 tggacgtcga agtttatcgg aaagtatggg gatcacagaa cgagtattgc gaacagaaac
    49141 agatcttttg aaaaatctga atttgatcga cacctcaaaa agcggtatga cgctgacaac
    49201 taagggcgaa gaagtttatc aaagccttga aaacttcatg gatcagcttt taggtacgca
    49261 tcaaaccgaa caacagttgg cagaatattt tggtaaccaa cgctgtatcg ttgtttcagg
    49321 aaacagcgaa gagcagacca aagtggctga tgcattcggc gaagcgctga gcgaagccct
    49381 agaccgacta cttccagaag gcgagaatat catcgctgtg atgggaggaa ctactatggc
    49441 tgtggtagcc gaacaattgt ctaatctgga aaataagaaa cggcataacc tgtttgtccc
    49501 tgcaagaggc gggattggtg aagcgataac tgtacaggca aattctgtca gtgcaagaat
    49561 ggctgctaaa gcaaacggta accaccgtgc actttatgtt ccggaacaat taagtctggc
    49621 aacttacaat tctttactca atgagccatc tatccaagaa gtcttaaact tgattagtga
    49681 agcgaattgt gttattcata gtattgggcg tgcattacac atggcagcac gtcgtaagat
    49741 gactgaaaaa gaattagtca tgctgaaaca agcaaatgct gttgcggaat cttttggcta
    49801 ctttttcaat gaaaaaggtg aagtcgttta taaggtccct agaatcggca tagaattgca
    49861 agatttggaa aaagtcccga tcatcatggc aatcgcgggc ggaaaatcaa aagctaaagc
    49921 aattcgagct tatatgaaaa atgcaccaaa acaaacgtgg ctcatcactg atgaagctgc
    49981 tgcaaatgag attttaaaag gggtaaccct ttaaaataaa tatttttttg attttcataa
    50041 ggaggaaatc ttaaatgaca gttaaagtag gtattaatgg ttttggacgt attggacgtc
    50101 tagcattccg tcgtatccaa gatgtagatg gaatcgaagt agtagcaatc aacgacttga
    50161 cagatgctaa aatgttggca cacttgttaa aatacgacac aactcaagga cgtttcaacg
    50221 gaacagttga agttcatgaa ggttcattca acgtaaacgg aaaagaagtt aaagtattag
    50281 ctaaccgcaa cccagaagaa ttaccatggg gcgaactagg cgtagatatc gttctagaat
    50341 gtactggttt cttcacttct aaatcagcag ctgaaaaaca tttaacagct ggtgctaaac
    50401 gcgttgttat ctctgctcct ggcggaaacg atgttccaac aatcgtttat aacacaaacc
    50461 acgaaacatt aactggtaaa gaaacagtta tctcaggtgc ttcatgtact actaactgtt
    50521 tagctcctat ggctaaaaca ttgaacgaca aatttggtgt tgttgaagga ttaatgacaa
    50581 ctatccacgc ttacacaggt gaccaaatga ctctagacgg acctcatcct aaaggtgact
    50641 tccgccgtgc acgcgctgct gctgcaaaca tcgttcctaa ctcaacaggt gctgctaaag
    50701 ctatcggttt agtaattcct gaattgaacg gtaaattaga cggagctgct caacgtgttc
    50761 ctgtaccaac aggttcatta acagaattag taacagttct tgaaaaagaa gtaactgttg
    50821 acgaaatcaa tgcagcaatg aaagaagctt caaacgaatc ttacggatac aacacagacg
    50881 aaatcgtttc ttcagatatc gttggtatga cttacggttc attatttgat gctacacaaa
    50941 ctaaagtaat gacagttggc gacaaacaat tagttaaaac tgttgcttgg tacgacaacg
    51001 aaatgtcata cactgcacaa ttagtacgta ctttagaata cttcgctaac ttataagata
    51061 gacaaacgta caagtgtaaa ttatagacat gtacatttaa aagcggggaa gcaatcgcgc
    51121 ttcttccgct ttttttgata ataagtttat tcaggaggaa ataaaatggc taagaaaaca
    51181 gtgaaagata tcgatttaaa agacaaaaaa gttcttgtcc gtgttgactt caacgttcct
    51241 ttgaaagacg gcgtgatcac tgacgacact cgtatcaaag cagcattgcc aacaatcaac
    51301 tacgttctag aacaaggcgg aaaagcaatc cttttctctc accttggacg tgtaaaaaca
    51361 gaagaagata aagaaggaaa atctttagct ccagttgcaa aacgtctagg cgaattatta
    51421 ggtaaagaag taacattcgt acctgaaact cgcggagaac aattagaaga agcaatccgc
    51481 aacatgaatg acggcgacgt tgtcgtattc gaaaatacac gatttgaaga tattgacggt
    51541 aaaaaagaaa gcaaaaatga tacagaacta ggtaaatact gggcttcatt aggcgatgta
    51601 tttgttaacg atgctttcgg tacagctcac cgtgcacatg cttctaacgt aggtatcgct
    51661 tcaactggta tcccaacagt tgctggattc ttgatggaaa aagaaatcaa gttcatcggt
    51721 gaagcagtag aagaaccaaa acgaccaatg attgcaatcc ttggtggcgc aaaagtttct
    51781 gataaaatcg gcgtaatcga aaacttgatt cctaaagcag ataaaatctt gatcggtggc
    51841 ggaatgactt acacattcta cgaagcaaaa ggaatcaaga tcggtaactc tctagtagaa
    51901 gctgataaag tggaactagc aaaagaatta atcgaaaaag caggcgacaa acttgtcttg
    51961 ccaatcgaca atgtctgtgc accagaattt tcaaatgatg ttgaaacaca agtaatcgaa
    52021 ggagacattc ctgatggttt gatggcttta gacatcggcc cagcatcagt aaaattattt
    52081 gctgatacat tacaaggtgc aaaaacagtt gtttggaacg gacctatggg tgtatttgaa
    52141 atgagtaact ttgctaaagg tacaatcggt gtttgtgaag caatcgctaa cttagaaggc
    52201 gcaacaacaa tcatcggtgg cggagactca gctgcagcag cagaacaatt aggatttgct
    52261 gacaaattca cacacatctc aacaggtggt ggcgcaagct tggaattgct tgaaggtaaa
    52321 gaattaccag gactagcagc aatcaacgat aaataaacga ccaagtttgc tcaattctga
    52381 cgagtaatcg atcaaaaggt gaagggagtt ttcatgactc cctagccatt tttcaataaa
    52441 ttaaggagtg tgttcctcaa tgcgtaaacc tattatcgca ggtaactgga aaatgaacaa
    52501 aactgcatca gaagcaaaag aatttgctga agcagtaaaa aacaaaatcc cttcaaatga
    52561 caaagttgat tcagtcatcg gatctcctgc attattctta caagaattaa tgacatcagc
    52621 tgaaggtaca gaactaaaaa tctcagcaca aaactgctac tgggaaaact caggtgcgtt
    52681 cactggtgaa acttcaccag cagctcttgc tgatctaggt gttcattatg tgatcatcgg
    52741 acattcagaa cgtcgtgaat atttccatga aacagacgaa gacatcaata aaaaagcaaa
    52801 agctatcttt gcaaatggta tgacaccaat cctatgctgc ggcgaatctt tagaaaccta
    52861 tgaagcaggc aaaactgctg aatggatcga aggacaaatt acagctggtt tagctgatct
    52921 ttcagctgaa caagtgtcaa acatggttat tgcttatgaa ccaatctggg caatcggtac
    52981 tggtaaatca gctgacgcac aaatcgctga cgaaatttgt ggagttgtac gtcaaacagt
    53041 tgaaaaactt tatggaaaag aagtatcaga agcagttcgt atccaatacg gcggttctgt
    53101 aaaaccagaa aatattgctg aatacatggc aaaagaaaat gttgacggtg ctttagtcgg
    53161 tggcgcaagc ttgcaagcag attcattttt gtcattgtta gacgctgtaa aataattgtt
    53221 gaaaatttgc gaaaaatgca agaaatgttt gcactaatga gcaaaattaa ctaaaatcgt
    53281 atatgaggga aatcccttag gaaaaaacaa actcaaagga gagacaaaac atgtcaatta
    53341 ttactgatgt ttacgctcgc gagatcttag actcacgtgg taacccaaca atcgaagtag
    53401 aagtatacac agaaagcgga gcttttggcc gcggaatggt tccttcaggt gcttcaactg
    53461 gtgaatacga agcagttgaa ttacgtgacg gagacaaagc tcgttatggc ggtaaaggtg
    53521 taactaaagc tgtcgacaac gtaaacaaca tcattgctga agcaatcatt ggctacgatg
    53581 ttcgtgacca aatggctatc gacaaagcta tgatcgcttt agacggaact cctaacaaag
    53641 gtaaattagg tgcgaacgct attttaggtg tttctatcgc tgttgcacgt gcagctgctg
    53701 actacctaga agtaccttta taccactacc taggcggatt caacacaaaa gtattgccaa
    53761 caccaatgat gaacatcatc aacggcggat cacatgctga taactcaatc gacttccaag
    53821 aattcatgat catgcctgtt ggcgctccaa cattcaaaga agctctacgt atgggtgctg
    53881 aagtattcca cgcattagct tcaatcttga aagctcgcgg attagcaact tctgttggtg
    53941 acgaaggtgg atttgctcca aaccttggtt caaacgaaga aggttttgaa gtaatcatcg
    54001 aagcaatcga aaaagctggc tatgtacctg gtaaagacgt tgttcttgct atggatgctg
    54061 cttcttcaga attctacgac aaagaaaaag gtgtttacgt actagctgac tcaggcgaag
    54121 gcgaaaaaac aactgacgaa atgatcaaat tctacgaaga attagtttct aaatatccaa
    54181 tcatctcaat cgaagatgga ttagacgaaa acgactggga cggattcaag aaattaactg
    54241 acgtattagg cgacaaagtt caattagttg gtgacgactt gttcgtaaca aacactcaaa
    54301 aattatctga aggtatcgaa aaaggaattg ctaactcaat ccttatcaaa gtgaaccaaa
    54361 tcggtacatt aacagaaact ttcgaagcta tcgaaatggc taaagaagct ggctacacag
    54421 cagttgtatc tcaccgttct ggtgaaacag aagattcaac aatctctgat atcgctgttg
    54481 caacaaatgc tggccaaatc aaaactggtt ctctatcacg tactgaccgt attgctaaat
    54541 acaaccaatt actacgtatc gaagaccaac ttggtgaagt tgcagaatac aaaggtttga
    54601 aatctttcta caacttaaaa aacaaataat tcttttctaa tgaagataca tgatcgacta
    54661 ttgaaagata gtcgagtatg atcacttagt taagtaatcg aactctctac ttgctgaaat
    54721 atcagcaggt agagagt
//
LOCUS       DAFCHQ010000010        51201 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000010 DAFCHQ010000000
VERSION     DAFCHQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.010"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            320..1165
                     /locus_tag="LNC71_000694"
     CDS             320..1165
                     /locus_tag="LNC71_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6465789.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1446..2666
                     /locus_tag="LNC71_000695"
     CDS             1446..2666
                     /locus_tag="LNC71_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBK6465790.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2663..5383
                     /locus_tag="LNC71_000696"
     CDS             2663..5383
                     /locus_tag="LNC71_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6465791.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5367..6311
                     /locus_tag="LNC71_000697"
     CDS             5367..6311
                     /locus_tag="LNC71_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6465792.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6380..7390)
                     /locus_tag="LNC71_000698"
     CDS             complement(6380..7390)
                     /locus_tag="LNC71_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6465793.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7570..7914)
                     /locus_tag="LNC71_000699"
     CDS             complement(7570..7914)
                     /locus_tag="LNC71_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK6465794.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7907..8341)
                     /locus_tag="LNC71_000700"
     CDS             complement(7907..8341)
                     /locus_tag="LNC71_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBK6465795.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8479..9216
                     /locus_tag="LNC71_000701"
     CDS             8479..9216
                     /locus_tag="LNC71_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465796.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9209..10420
                     /locus_tag="LNC71_000702"
     CDS             9209..10420
                     /locus_tag="LNC71_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465797.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10624..11406
                     /locus_tag="LNC71_000703"
     CDS             10624..11406
                     /locus_tag="LNC71_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBK6465798.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11476..12138
                     /gene="trmB"
                     /locus_tag="LNC71_000704"
     CDS             11476..12138
                     /gene="trmB"
                     /locus_tag="LNC71_000704"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBK6465799.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12236..13990)
                     /gene="ade"
                     /locus_tag="LNC71_000705"
     CDS             complement(12236..13990)
                     /gene="ade"
                     /locus_tag="LNC71_000705"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBK6465800.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14122..15051
                     /gene="tadA"
                     /locus_tag="LNC71_000706"
     CDS             14122..15051
                     /gene="tadA"
                     /locus_tag="LNC71_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBK6465801.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15008..16057
                     /locus_tag="LNC71_000707"
     CDS             15008..16057
                     /locus_tag="LNC71_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBK6465802.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16054..16332
                     /locus_tag="LNC71_000708"
     CDS             16054..16332
                     /locus_tag="LNC71_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBK6465803.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16307..16777
                     /locus_tag="LNC71_000709"
     CDS             16307..16777
                     /locus_tag="LNC71_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBK6465804.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16794..17096
                     /locus_tag="LNC71_000710"
     CDS             16794..17096
                     /locus_tag="LNC71_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465805.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17113..17484
                     /locus_tag="LNC71_000711"
     CDS             17113..17484
                     /locus_tag="LNC71_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBK6465806.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17456..17815
                     /locus_tag="LNC71_000712"
     CDS             17456..17815
                     /locus_tag="LNC71_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465807.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17987..18994
                     /locus_tag="LNC71_000713"
     CDS             17987..18994
                     /locus_tag="LNC71_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6465808.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19019..20203
                     /locus_tag="LNC71_000714"
     CDS             19019..20203
                     /locus_tag="LNC71_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBK6465809.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20640..22223)
                     /locus_tag="LNC71_000715"
     CDS             complement(20640..22223)
                     /locus_tag="LNC71_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6465810.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22801..22944)
                     /locus_tag="LNC71_000716"
     CDS             complement(22801..22944)
                     /locus_tag="LNC71_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBK6465811.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23167..23646)
                     /locus_tag="LNC71_000717"
     CDS             complement(23167..23646)
                     /locus_tag="LNC71_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6465812.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23719..24003)
                     /locus_tag="LNC71_000718"
     CDS             complement(23719..24003)
                     /locus_tag="LNC71_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465813.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24081..24275)
                     /gene="ssrS"
                     /locus_tag="LNC71_000719"
     ncRNA           complement(24081..24275)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LNC71_000719"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24347..25633)
                     /locus_tag="LNC71_000720"
     CDS             complement(24347..25633)
                     /locus_tag="LNC71_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBK6465814.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25771..26442
                     /locus_tag="LNC71_000721"
     CDS             25771..26442
                     /locus_tag="LNC71_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBK6465815.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26408..26896
                     /locus_tag="LNC71_000722"
     CDS             26408..26896
                     /locus_tag="LNC71_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBK6465816.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26909..27856
                     /gene="prmA"
                     /locus_tag="LNC71_000723"
     CDS             26909..27856
                     /gene="prmA"
                     /locus_tag="LNC71_000723"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBK6465817.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27857..28621
                     /locus_tag="LNC71_000724"
     CDS             27857..28621
                     /locus_tag="LNC71_000724"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBK6465818.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28854..31067
                     /locus_tag="LNC71_000725"
     CDS             28854..31067
                     /locus_tag="LNC71_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBK6465819.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31078..31524
                     /locus_tag="LNC71_000726"
     CDS             31078..31524
                     /locus_tag="LNC71_000726"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBK6465820.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31695..32954
                     /locus_tag="LNC71_000727"
     CDS             31695..32954
                     /locus_tag="LNC71_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBK6465821.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33043..33990)
                     /locus_tag="LNC71_000728"
     CDS             complement(33043..33990)
                     /locus_tag="LNC71_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6465822.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34384..35676
                     /locus_tag="LNC71_000729"
     CDS             34384..35676
                     /locus_tag="LNC71_000729"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBK6465823.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35691..37460
                     /gene="aspS"
                     /locus_tag="LNC71_000730"
     CDS             35691..37460
                     /gene="aspS"
                     /locus_tag="LNC71_000730"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBK6465824.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37569..38444
                     /locus_tag="LNC71_000731"
     CDS             37569..38444
                     /locus_tag="LNC71_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6465825.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38710..40593
                     /locus_tag="LNC71_000732"
     CDS             38710..40593
                     /locus_tag="LNC71_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK6465826.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40668..40952)
                     /locus_tag="LNC71_000733"
     CDS             complement(40668..40952)
                     /locus_tag="LNC71_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK6465827.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40942..41676)
                     /locus_tag="LNC71_000734"
     CDS             complement(40942..41676)
                     /locus_tag="LNC71_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBK6465828.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41805..42428
                     /locus_tag="LNC71_000735"
     CDS             41805..42428
                     /locus_tag="LNC71_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBK6465829.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42620..43741
                     /locus_tag="LNC71_000736"
     CDS             42620..43741
                     /locus_tag="LNC71_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBK6465830.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43748..46876
                     /locus_tag="LNC71_000737"
     CDS             43748..46876
                     /locus_tag="LNC71_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBK6465831.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46895..50089
                     /locus_tag="LNC71_000738"
     CDS             46895..50089
                     /locus_tag="LNC71_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBK6465832.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50235..50942)
                     /locus_tag="LNC71_000739"
     CDS             complement(50235..50942)
                     /locus_tag="LNC71_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6465833.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16752 a         8945 c        10636 g        14868 t
ORIGIN      
        1 gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt gaactaatag
       61 agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat agttggttat
      121 tataaaatag taatatagct gatttggatt caattcatat ttatttataa atcaaaaata
      181 cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta cttacttaaa
      241 ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc agtgaattta
      301 taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt tagacggtac
      361 tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga aacatttacc
      421 ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta ataatattct
      481 caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg gatttgatgg
      541 agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa ttgattctgt
      601 cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat ctcagaatta
      661 taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta tcaaagagct
      721 aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata atattgagga
      781 aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca
      841 attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc
      901 ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg
      961 tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa
     1021 tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga
     1081 aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc
     1141 atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa
     1201 tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc
     1261 gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat
     1321 aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt
     1381 tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat
     1441 ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac
     1501 tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat
     1561 caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata
     1621 attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac
     1681 ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa
     1741 aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag
     1801 agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc
     1861 gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt
     1921 accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt
     1981 tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg
     2041 tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata
     2101 ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc
     2161 aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga
     2221 aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg
     2281 atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt
     2341 tggaacggat acaatcagga gagctattag actatttgtt agaggaattc tcagatagga
     2401 taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc
     2461 cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag
     2521 atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg
     2581 aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt
     2641 ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa
     2701 atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga
     2761 agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa
     2821 aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg
     2881 gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg
     2941 aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat
     3001 cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat
     3061 ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac
     3121 gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt
     3181 caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca
     3241 agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga
     3301 acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca
     3361 attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct
     3421 caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt
     3481 ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa
     3541 gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc aggagcagga
     3601 actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag
     3661 attacaaatt gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca
     3721 ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct
     3781 ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc
     3841 taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc
     3901 attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat
     3961 ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct
     4021 tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa
     4081 aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga
     4141 tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa
     4201 acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga
     4261 tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg
     4321 cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga
     4381 ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa
     4441 aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta
     4501 tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg
     4561 gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc
     4621 tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc
     4681 ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact
     4741 gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa
     4801 tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact
     4861 ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc
     4921 acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt
     4981 accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca
     5041 gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat
     5101 ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct
     5161 ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta
     5221 tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca
     5281 aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat
     5341 cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc
     5401 agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa
     5461 aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg
     5521 cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa
     5581 gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag
     5641 atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg
     5701 aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc
     5761 gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc
     5821 atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat
     5881 caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc
     5941 atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag
     6001 gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt
     6061 tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt ttagcacacc
     6121 atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac
     6181 atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag
     6241 aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa
     6301 aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct
     6361 tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt gaagattctt
     6421 ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt tcacttgagt
     6481 ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag gcatcatctt
     6541 tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg aatgcattgt
     6601 cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta tccatgtcat
     6661 tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca taagaagtag
     6721 gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa gctgcttctt
     6781 ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg ccatcttctt
     6841 ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct ccatcatccg
     6901 ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt tgtgcttcta
     6961 cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata tctacgtgag
     7021 ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa gtatctttcg
     7081 tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc gcttgtttgt
     7141 cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta ttgaatactt
     7201 tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt gcttcatcat
     7261 agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta ttagtatcgc
     7321 ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa ataattgatt
     7381 tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata ttaactatac
     7441 catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt caaaatttca
     7501 tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct gttgagaaaa
     7561 taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt tttctacttg
     7621 ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga tatctttttt
     7681 gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt cgatcaatgc
     7741 gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg cttcttccaa
     7801 ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata acagacctcc
     7861 gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa tcatcgcagg
     7921 acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca taactatctt
     7981 gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg actaaatgga
     8041 tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag atattaagac
     8101 ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca cgcgaaaaaa
     8161 cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt ttaggcacaa
     8221 ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc ttgtcgtctt
     8281 catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata cagttttcca
     8341 tataaaacat cctttctata aagaataaat acttcagctt aactttacca taagttaaag
     8401 ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata aataaaaata
     8461 aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg gtacggacat
     8521 ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt agggttgatt
     8581 ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct gctggaagct
     8641 tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga agaatatcgc
     8701 aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac attgagagaa
     8761 catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat gaaaagagca
     8821 gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc agctaatttt
     8881 tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga gccttcttta
     8941 tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc attgcttgaa
     9001 ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca tattttagct
     9061 actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat acgagcagac
     9121 ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt ggacgaaatc
     9181 tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac gtttagcaag
     9241 acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt ttgttttagt
     9301 ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga aaaccctgca
     9361 gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct gtttattcgt
     9421 gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc ctaaagaaaa
     9481 agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc cgttgatctt
     9541 tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg agcaatcctt
     9601 ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc atctgcaaat
     9661 caagagagca gaattgtttc gactagatca acaagttatt cgttattgga aattaggctg
     9721 gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat gggcaggaat
     9781 agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc aacaagaaaa
     9841 aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc agatctatca
     9901 atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa gaagacgcta
     9961 tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc tttacttgtt
    10021 tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat tagtaattat
    10081 aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta tcggtttctt
    10141 atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc gctatatggt
    10201 tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc agtatctact
    10261 cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag tcgtattaga
    10321 tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc tctttgttaa
    10381 actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct gtgtagggta
    10441 aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct attttttttg
    10501 agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt gcttgacgtg
    10561 tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag gacgggagaa
    10621 tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa aggcgacaca
    10681 ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg aaacacgact
    10741 ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg gacaaaacga
    10801 acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt gctgactcct
    10861 aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca tcattcaaat
    10921 tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga gaagatgctg
    10981 caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct gcaaattgtc
    11041 catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc agtagtacac
    11101 ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct agtcgattgg
    11161 gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg aaattatgtg
    11221 ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac tgacgaaatg
    11281 cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac cagatattgc
    11341 aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg aattttcagt
    11401 ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat tagaggaaag
    11461 gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat gttagcggct
    11521 cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa tgaattgttt
    11581 gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt catcactgga
    11641 atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt cagtgttgtt
    11701 tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact tctacatgtg
    11761 gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat ctatctgaat
    11821 ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata taagactttt
    11881 cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa aacagataat
    11941 caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat cctcaaacag
    12001 gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga gtatgaagaa
    12061 aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca agataagacc
    12121 aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat atccataaat
    12181 aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat ttgatttaaa
    12241 atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga gaaaatgaaa
    12301 ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag aaagaaagtg
    12361 tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt attaattcat
    12421 tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct ataggaagag
    12481 aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct ttttgaatca
    12541 aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact agattatgcg
    12601 agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct ttcactatgc
    12661 ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt aattggtctt
    12721 tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc aaatctttag
    12781 tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa agaagcagct
    12841 ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac gtatatgaat
    12901 ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact aatgttccct
    12961 tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag aaatcagctt
    13021 gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct gcattcaatg
    13081 ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga cggacgatat
    13141 gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg acaaagaagc
    13201 atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct tttgcaacgg
    13261 tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct tcttttgcag
    13321 ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac tcttccatgc
    13381 cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat tggatctttt
    13441 ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca gctaagccta
    13501 aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt gcaccattgg
    13561 tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc atgagtgttt
    13621 gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt cctatttcat
    13681 ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa aattcgcttg
    13741 gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc actagatatt
    13801 gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg atcattcctt
    13861 cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg atcttagcat
    13921 gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat ttccttaatg
    13981 ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata aattcggttt
    14041 ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa aagacgtatg
    14101 ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat gataagtgaa
    14161 agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt attttacatc
    14221 tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc agatggacag
    14281 caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa aagaaaagct
    14341 cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg gttgtcttct
    14401 gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg tacttttgaa
    14461 acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa aaccgtcaga
    14521 cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct aatgtatcgg
    14581 ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga gatagaagaa
    14641 ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga tacacttttg
    14701 agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag agatgaatca
    14761 actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc aaccgtacat
    14821 gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa aaaagaagag
    14881 ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc caatcaccat
    14941 ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa atcatgggag
    15001 gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta aaggattaac
    15061 aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat ctggttttag
    15121 tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg ttattttggc
    15181 aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt cgcaaatagg
    15241 atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga atctgccgac
    15301 aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca atgatttgaa
    15361 aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct tgtttagtat
    15421 gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag agcattgggg
    15481 tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag cggttactct
    15541 attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc agagtgaatg
    15601 gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg cttatttgtc
    15661 actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca tagtctgctt
    15721 aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga ttcaaggatt
    15781 ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat cagaagaatt
    15841 cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac tgatttttta
    15901 cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc attttattca
    15961 gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg ctgttttatt
    16021 acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat atcttattat
    16081 agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt attagtgttg
    16141 ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag tcgtgaagcg
    16201 atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa tggaagagtt
    16261 cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc agaaatctat
    16321 cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat cactcttact
    16381 gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg catggaagaa
    16441 tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt ctacgcaaaa
    16501 aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag agaatcaaat
    16561 catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata ttcctgatga
    16621 gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga atgaaagttc
    16681 actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt atcaattcca
    16741 gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt ttgttggagt
    16801 cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt ggccagctgt
    16861 ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga atgatttatg
    16921 aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa gaaatgatcc
    16981 catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt gtcaatatgg
    17041 agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct gattgaatgt
    17101 ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt gattcagcaa
    17161 accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct aatattttta
    17221 gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa tcagcaactg
    17281 cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat gatacgtaaa
    17341 agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct tcaattattg
    17401 gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga tggatatgcc
    17461 atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat tttctgtctt
    17521 gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct accgtttgtc
    17581 aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga agcagatggc
    17641 tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact acacaactgg
    17701 atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag tctcaccgtt
    17761 tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag aatgatggaa
    17821 tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa aatgtttgcc
    17881 gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc gatagaatta
    17941 aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt taccagaaaa
    18001 aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga atgcattggg
    18061 aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg attatcaagt
    18121 acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag ctctttatga
    18181 agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc aattactttt
    18241 attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc ctgatagtat
    18301 cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag caccggttaa
    18361 gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt taaacctatc
    18421 gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat tgttggctgt
    18481 cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc aagacgggtt
    18541 gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa tcggttatta
    18601 tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc atagttatgc
    18661 acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt ttggtttgtt
    18721 tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa aatggtttaa
    18781 agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc gtaataaaca
    18841 atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac aagtaaaaga
    18901 agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg aatttttcaa
    18961 cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg agagtcatat
    19021 gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat tatatcaaat
    19081 gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac aagattctat
    19141 ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata tcgaaaacca
    19201 cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt taagctctta
    19261 tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt atggggattc
    19321 agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat ttgctccttt
    19381 gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac ctgacatttt
    19441 gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa attatttata
    19501 tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct tccatggcac
    19561 aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga tcgaagaact
    19621 aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg atggaggtaa
    19681 atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg gtacacgttc
    19741 aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga ttgatattga
    19801 tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag gattgtctag
    19861 tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag cttatgacat
    19921 tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga atggtgttga
    19981 tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta gtgaaatcat
    20041 caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat tgcgtggtga
    20101 agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc caacagatga
    20161 ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag cattcgttag
    20221 gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt gcggagaaga
    20281 ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt cttagggaat
    20341 tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg attttcagga
    20401 aaatcccaat attttatttt cattcatctt tataagggac attacatatg taaaaagaaa
    20461 aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta tgtttagcta
    20521 gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc cacctgtact
    20581 ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga aaaaacgtcc
    20641 tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat aacgagtgct
    20701 tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag cgttccttga
    20761 tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg attggttggt
    20821 tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc caaacggatt
    20881 ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata aaagccactt
    20941 ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc gtaagccagc
    21001 aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat ttgcaagcta
    21061 ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa tttgagtaag
    21121 gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg atagactgca
    21181 aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt ttgaatgatg
    21241 aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt tttcggggga
    21301 ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt ggcttttttg
    21361 aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat catgactttc
    21421 aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc caagcgagct
    21481 tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc accttcttcc
    21541 agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga aacgaccaga
    21601 accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt aagatctaaa
    21661 tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat tttggctaaa
    21721 gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg acggtgtgga
    21781 atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc gcctagttct
    21841 tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt tgtatctggt
    21901 tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat ctcagcggca
    21961 gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg ttcgaccgtc
    22021 ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc tctgccggtc
    22081 aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc aattttttca
    22141 ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat tgttttttta
    22201 atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa aggccatggt
    22261 tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct tgagatggcc
    22321 attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca gcaactgtaa
    22381 aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta cttgttattt
    22441 tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc agtgtacctg
    22501 aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga tgtgtaattc
    22561 tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca tcattgtatt
    22621 ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac tttgtctaca
    22681 tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct ctttggaaat
    22741 ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg tagcctttca
    22801 tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc cgaacccgaa
    22861 ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat attttttcgt
    22921 atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat gcttaggtcg
    22981 ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta ttttacttga
    23041 actcattgac tgttttccct ttattttaaa cattataaag accgatgagt gcttgccgcg
    23101 tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg gcaggtcgtc
    23161 tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat cagttcggac
    23221 aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac caataaataa
    23281 acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt gattatcttc
    23341 tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag aggttacatt
    23401 attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct ttgccatttc
    23461 agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag aacgtgtgtc
    23521 aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga ccgccttttt
    23581 aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt tgtattgttg
    23641 taacataagc attcactcct caagtcaatc gtattgtact cttttattgt agaacacttt
    23701 agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa atcggatact
    23761 gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa agcgttgatt
    23821 gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc cgtaaaattg
    23881 taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat cctgtttatt
    23941 aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta aaatcctaat
    24001 catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca agagcaaatt
    24061 gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct gtagtattga
    24121 tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca aatgataagg
    24181 catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg ttcggtcaac
    24241 acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc atatgaattt
    24301 aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag ttttttggag
    24361 gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt tcatatttcc
    24421 ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa tattgctgat
    24481 tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg ttgagtgatt
    24541 tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct ttgccctctc
    24601 gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt ggcgacaagc
    24661 aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc aatttttcag
    24721 cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa cctatatctt
    24781 cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc tcaactaaac
    24841 gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt tgaaaagccg
    24901 aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt ctttggacac
    24961 attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa ggagtcgata
    25021 gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt gcacgcgaaa
    25081 ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg cgttctttat
    25141 cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt ttgacttcaa
    25201 agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc tcagtagtcg
    25261 ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc tgttttgttt
    25321 tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt tttgcttctt
    25381 ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg cgaaatgcat
    25441 aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca ggaggtccat
    25501 atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt tttccttccc
    25561 cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg cgataagcca
    25621 atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt atatcatatt
    25681 tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct tatccaacta
    25741 atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat agatgaattt
    25801 ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta tttggagcat
    25861 gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata cctaggtcca
    25921 gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg ggcaatgtat
    25981 gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt gaacatcatt
    26041 actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc cgccgcaatg
    26101 attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag caacggtccg
    26161 ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc tatagcagat
    26221 agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa tgctttgcct
    26281 agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt tgttgtgtcg
    26341 ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg gggttggagg
    26401 aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg aaaaacaagt
    26461 gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg agatcgttgt
    26521 tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag ggatcgtttc
    26581 gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc agaaatctgt
    26641 ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag ggttggcaaa
    26701 atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag ggcaaagcga
    26761 tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc actgggataa
    26821 tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata cttatttacc
    26881 gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag tagaaactga
    26941 aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga gtggtgtcgc
    27001 gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg agattttaga
    27061 taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt ttccggaaac
    27121 gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat tacctgagtt
    27181 tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa gcaactgggc
    27241 aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga cggttgcgcc
    27301 aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac tagatcctgg
    27361 tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag cactagaaac
    27421 agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg tgctaagtat
    27481 tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg aagtagccgt
    27541 tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc atgtatcagc
    27601 taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga acattttagc
    27661 agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa caggcacgct
    27721 gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca tgttagaaca
    27781 agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta ttttgaaaaa
    27841 accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat caaaagacta
    27901 ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga tggaacccga
    27961 ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa tcacggagat
    28021 tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa aggaactgcc
    28081 aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt ggatcgttca
    28141 gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag cctctgtcgt
    28201 taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga ttgctcaaga
    28261 ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg aaacgaaaca
    28321 agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg aggaatcagc
    28381 aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac cagatagtcg
    28441 aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg attattttgt
    28501 agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag aaacagcacc
    28561 tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt ctttcagcta
    28621 acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt caacaatttg
    28681 tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa agcttttaaa
    28741 aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa ttatcaaaca
    28801 gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt cctgtgacaa
    28861 aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac atgggtcccg
    28921 aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat gacggacagt
    28981 ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt atattagcag
    29041 atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt gttgaagata
    29101 cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct atgttagtgg
    29161 acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag ctagcagaaa
    29221 accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg gtcaaattag
    29281 ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag caaagaagga
    29341 tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg atcagccgga
    29401 tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag tattaccgta
    29461 tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt ggtactgttg
    29521 aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat ggacggccaa
    29581 aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc aacgaaattt
    29641 atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat gctgtattag
    29701 gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat attgccatgc
    29761 caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca ggaaatccgg
    29821 tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg gttgctgcac
    29881 actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg acgaaacaat
    29941 taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct tctgaattta
    30001 tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca ccaaaaggag
    30061 acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt atccatacag
    30121 atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag cttgattaca
    30181 aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt ggcccaagtc
    30241 gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa cgtttcttca
    30301 aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc aaatgtatta
    30361 cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa gaagcactcg
    30421 atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat ggagaagtta
    30481 gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa aaaatcgagc
    30541 agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct gacaaaatga
    30601 aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta attcgtatca
    30661 gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact aaaggcagag
    30721 ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca aatgtagcag
    30781 aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag tatgatgcag
    30841 atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt caaacagtca
    30901 atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat aaaatggcga
    30961 cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc gtggataaaa
    31021 tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag gacagatatg
    31081 agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca agaaattgga
    31141 aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac gcaaaaagat
    31201 gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga acaaggaaaa
    31261 atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca attcacactg
    31321 tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg accagaaaca
    31381 gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat cacagtcgct
    31441 accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc tgtaacgatc
    31501 attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa ctatgaaaca
    31561 cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct gcaaaaagcg
    31621 cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt attttttata
    31681 ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca gatttaaaaa
    31741 aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg gacgctcccg
    31801 tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca tcagcagcaa
    31861 aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa ttagtattga
    31921 agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat caccatcgga
    31981 aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat cgtatcttag
    32041 ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta tttgatttaa
    32101 gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta tcagcgattt
    32161 ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc ggaacgttta
    32221 cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa aaatgggcgc
    32281 aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat gtggcattat
    32341 atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa gtggcagctg
    32401 ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg gcattgatgg
    32461 gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac ttttcgacgt
    32521 tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca atgctacctg
    32581 cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc agttgggtta
    32641 ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca gattccttta
    32701 tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa tatgagatga
    32761 ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa tggatcactc
    32821 ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc tctgtacctg
    32881 aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc cgagcgctac
    32941 tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa cagtgctgat
    33001 cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc cacgaaatgc
    33061 ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt ggacattgtc
    33121 agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg gattcttcat
    33181 gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa tggggcgaag
    33241 tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg gatttctttt
    33301 tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc ctgtcagcaa
    33361 accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa gataagggaa
    33421 taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta catcgacata
    33481 tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac cgactgctcg
    33541 gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt ttggtgtgct
    33601 ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac gttttagtgc
    33661 atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc ctttatttgg
    33721 tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt tttccttcag
    33781 tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg tatcgatcaa
    33841 attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat cttccaaacc
    33901 gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt ctgcgtagac
    33961 ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt ctagtctaaa
    34021 acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc agatatctct
    34081 tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat gaaagagcga
    34141 gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc taaagtttca
    34201 ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta cgagcgttat
    34261 tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa tttcattcac
    34321 ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa gggagagaaa
    34381 actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga atcagaaaaa
    34441 tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta taatgaaatg
    34501 cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga tacaacggat
    34561 atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt gacgcttcga
    34621 ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt cggcccagag
    34681 tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga acgccctcaa
    34741 aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc agaaaaccct
    34801 gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt ggggatcacg
    34861 caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc ctatcgtcaa
    34921 gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc taaacgccgc
    34981 cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca aaaatttgtg
    35041 gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca ttttgatacc
    35101 gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa tatggttcgc
    35161 ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc aaaaatgggg
    35221 gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga actaggtggt
    35281 ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt gactatggag
    35341 gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt cgcactcgga
    35401 gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt tggtttttca
    35461 gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc agataaagcg
    35521 aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac gattaatgtc
    35581 aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata tgaacatttt
    35641 gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa atggcacaaa
    35701 gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt acattaaaag
    35761 gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta cgtgatcgtg
    35821 aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg gaaatcgcgg
    35881 ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga cgcggagaaa
    35941 atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt gaaatcgtga
    36001 ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca atcagtgacg
    36061 aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact aaaaacatca
    36121 aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat gactttctcg
    36181 atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac tacctagttc
    36241 cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa ttgtttaaac
    36301 aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt ttccgcgatg
    36361 aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa acaacgttct
    36421 taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta atgaaagaag
    36481 ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa gcaatggctc
    36541 gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat ctaagcgata
    36601 cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc ggtgtcgtga
    36661 aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg gatcagttag
    36721 gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta gaagaagacg
    36781 gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc attaaagcaa
    36841 cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa atcgtcgctg
    36901 ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt gatgaatcga
    36961 aattcaattt cttatgggta actgattggc cacaatttga attttcagaa gaagaaggtc
    37021 gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc ccgttattgg
    37081 ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac ggttatgaat
    37141 taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta ctagaaactc
    37201 taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca gctttagatt
    37261 acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca atgttattag
    37321 ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa gcagcagatg
    37381 tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg aatattgatg
    37441 taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa tacgcttctt
    37501 ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat ataaagcggg
    37561 gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg accaagcttt
    37621 cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt tatcagccag
    37681 gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact ttgtcaaaat
    37741 cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat atacctttgt
    37801 ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt atcacagtca
    37861 ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc gatccaatca
    37921 tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg ttgagaaacg
    37981 gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa acaggaaagt
    38041 cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc ggaattctat
    38101 ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga aaagtaactg
    38161 atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa catccagaaa
    38221 aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc aatgaagcag
    38281 aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca cgatacgaac
    38341 tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta agtattgcag
    38401 aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag tttgacaaaa
    38461 attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt ctaccatagt
    38521 agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg aaaaaaagca
    38581 tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag gacacgattc
    38641 tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta tgaaaagttg
    38701 gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac tgggtttgat
    38761 gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc aactagtatg
    38821 aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt gatcacggat
    38881 ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt ttatgtcaga
    38941 agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc ggcttatatc
    39001 actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag cagcttactg
    39061 tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg atttgctaat
    39121 gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta tttggtttgg
    39181 ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat cttttatact
    39241 gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac accaggaggc
    39301 gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg gttgggaagt
    39361 gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa acaagcgaaa
    39421 aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg cttcttcttt
    39481 ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag tctgttatgc
    39541 gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg gctgacaaat
    39601 ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca gtttccagtt
    39661 atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt gcatacttta
    39721 gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt ggagcgtccg
    39781 ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt cagcaaaccg
    39841 atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc aattgcactc
    39901 gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca actgcaggca
    39961 aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga aaacaccact
    40021 agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca agggaataag
    40081 gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc tgcaacggat
    40141 ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca attatatgcc
    40201 agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac tgttctgatt
    40261 ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat ggatgcaatc
    40321 ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg gcaaaatgac
    40381 gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct gattgattgg
    40441 tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca tccaaatcct
    40501 gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg aagttcaagt
    40561 acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc aggcaaaaac
    40621 atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca tatagattca
    40681 ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg ggtgagtttt
    40741 ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc tgcatggatc
    40801 atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc attgttatgt
    40861 tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg atctttacag
    40921 aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct ctgcagtgta
    40981 ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt ctaatttgcc
    41041 ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg ggtaaacaaa
    41101 ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt ctaagaaccg
    41161 atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac ttgaaatatc
    41221 gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt gtttattagg
    41281 gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga ctaagtcaca
    41341 agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga cagtcatttg
    41401 ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac gaggttgaag
    41461 ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa ctgcgccatt
    41521 tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt gagcaagcag
    41581 cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga tatcttctgc
    41641 gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa atgaaaaaaa
    41701 tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt caaacctcta
    41761 ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt tatcgattta
    41821 tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc tatgagaata
    41881 aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt acttggtggg
    41941 acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg gcaaaagaag
    42001 aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct tttccagtta
    42061 aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg aaatcgacaa
    42121 atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt aaaggcggaa
    42181 tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat caaggtccgt
    42241 tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc gaaccaatcg
    42301 acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa cgacgaacac
    42361 aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat gaagtcaaaa
    42421 aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc ctgttcagtt
    42481 cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt tgcgttccaa
    42541 tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata attagaagga
    42601 cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata tcggaaaaaa
    42661 actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta tactagatat
    42721 cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg ataggagtgt
    42781 cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga atttaaagga
    42841 aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac tttcagcggg
    42901 tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag aagcatttga
    42961 accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac caattgcagc
    43021 gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag ccaaggtgat
    43081 agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta gccatttttt
    43141 tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg ggttggattc
    43201 gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt tgcataatca
    43261 agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat tctctttatc
    43321 tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc tggagttgaa
    43381 ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt tcaaagaatt
    43441 gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata tctatctcac
    43501 tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc ctcgtatttt
    43561 ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga
    43621 aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg aagagcagct
    43681 aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg
    43741 atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta tcaatgagac
    43801 cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga agacaggagc
    43861 tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa cttccggaag
    43921 acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt
    43981 gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac cagaacaagt
    44041 tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa cgttgaccat
    44101 ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag cagatcagtt
    44161 gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt tactacccca
    44221 aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg ttttacgtca
    44281 tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa aagcaaaaca
    44341 acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa aaaggtttgt
    44401 tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc aatggacaaa
    44461 ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa cggaacttca
    44521 ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga aaaaaagtta
    44581 tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa aagacgaaat
    44641 agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag tcgaaccaat
    44701 ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa gtagtgagca
    44761 aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa atgaagcaaa
    44821 ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa acaaacagat
    44881 gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac aattaacaat
    44941 ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg ctatctatca
    45001 ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc aagaagatcg
    45061 tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag aaaaacaaaa
    45121 agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag caactgaaga
    45181 aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac agctgaaaag
    45241 ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag gggagccatg
    45301 tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac agccaaataa
    45361 ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg tagaaagaac
    45421 tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac aattatcaga
    45481 tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag aacacttgca
    45541 acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa caactcttgt
    45601 agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa gaatagagct
    45661 ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct acaaacagca
    45721 gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa gtttagaaca
    45781 acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac ttgaaaaaca
    45841 gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc aattgttgca
    45901 agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag aaaaaataga
    45961 tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc agtcgtattt
    46021 ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata aagaaaacca
    46081 acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc tgcgtcaatt
    46141 agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc aacagttaga
    46201 agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac aagaacaaaa
    46261 aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa gccagacgaa
    46321 attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag aaaatcctaa
    46381 gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt tacaagtagc
    46441 aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag cggataccat
    46501 tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc aggcaggtat
    46561 gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac tttctttagc
    46621 attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg atgcattgtt
    46681 tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa tggaagcttt
    46741 agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa gagaattgaa
    46801 agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa gcaaaatacg
    46861 ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc cagaaaaagt
    46921 cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg tccctgacca
    46981 agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg ttgatttgga
    47041 tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac ataaatattg
    47101 taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc gtatcatgaa
    47161 aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa
    47221 aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt tgattattga
    47281 ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta ttttggcact
    47341 ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta
    47401 tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac agtaccagtt
    47461 tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg
    47521 aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag gaaaactaga
    47581 taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc agatgaatca
    47641 gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac aattttccga
    47701 tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt tgctgcaagc
    47761 attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc aagggacatt
    47821 ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat tgattcgccg
    47881 attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat
    47941 tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag aagaggaagt
    48001 agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc
    48061 tcgagtcgat ttcgtctatg gtgaagtcat ctattcgact gacccgaagc atgaggtctc
    48121 gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag tacttgatct
    48181 attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat tgccaagcgg
    48241 ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt ttggtgaagt
    48301 cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc aaccgaaaat
    48361 cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag ggatagagga
    48421 gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt atacattaga
    48481 aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc aagtgttgca
    48541 acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta aaaatcaagg
    48601 attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat cgttccaaac
    48661 gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca aaggattgaa
    48721 tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta
    48781 tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac agacaattgc
    48841 ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg tagcgcctgc
    48901 gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt taagtatcca
    48961 agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca cagatatccg
    49021 agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc aaaaaattga
    49081 ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc
    49141 attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa tcgagaataa
    49201 tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc cgactaaaac
    49261 aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt ttattttacg
    49321 gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga atctatattc
    49381 tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga
    49441 aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac ttgctggtct
    49501 gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt accaaggcgg
    49561 ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga acaaacgaag
    49621 agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag agttagccga
    49681 gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc ggttaagtat
    49741 ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa aggctggggg
    49801 aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt ggtggaatcc
    49861 tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga
    49921 agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac tccagcaaga
    49981 aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga cgaaactgac
    50041 tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag agactcgaaa
    50101 aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat ttgatttaaa
    50161 aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca tttttttatt
    50221 tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg atcaaaagaa
    50281 gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg gttgaaccaa
    50341 agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt atcgccaacg
    50401 tataaggtct cttctggaag catatgaaat tctttctcag ccagctgaaa aatctctttt
    50461 tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg aatccaattg
    50521 ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt tgtgataatt
    50581 cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag
    50641 cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc ttcttccgta
    50701 atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg catatattct
    50761 agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg gcggaaccgg
    50821 atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt gaccgcgttt
    50881 cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac tgatttaatc
    50941 aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga ctattgtaac
    51001 agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat ttctatttcc
    51061 ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga aacacgtcaa
    51121 taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt ttaatgcaat
    51181 ctctgtggaa ttttcgaaag a
//
LOCUS       DAFCHQ010000011        46080 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000011 DAFCHQ010000000
VERSION     DAFCHQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.011"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..933
                     /locus_tag="LNC71_000740"
     CDS             52..933
                     /locus_tag="LNC71_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465834.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            946..2556
                     /locus_tag="LNC71_000741"
     CDS             946..2556
                     /locus_tag="LNC71_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBK6465835.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2633..3292)
                     /locus_tag="LNC71_000742"
     CDS             complement(2633..3292)
                     /locus_tag="LNC71_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6465836.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3575..4240)
                     /locus_tag="LNC71_000743"
     CDS             complement(3575..4240)
                     /locus_tag="LNC71_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBK6465837.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4387..4992)
                     /gene="dhaL"
                     /locus_tag="LNC71_000744"
     CDS             complement(4387..4992)
                     /gene="dhaL"
                     /locus_tag="LNC71_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBK6465838.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5020..6030)
                     /gene="dhaK"
                     /locus_tag="LNC71_000745"
     CDS             complement(5020..6030)
                     /gene="dhaK"
                     /locus_tag="LNC71_000745"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBK6465839.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6005..6451)
                     /gene="dhaM"
                     /locus_tag="LNC71_000746"
     CDS             complement(6005..6451)
                     /gene="dhaM"
                     /locus_tag="LNC71_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBK6465840.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6554..6874
                     /locus_tag="LNC71_000747"
     CDS             6554..6874
                     /locus_tag="LNC71_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBK6465841.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6986..7855)
                     /locus_tag="LNC71_000748"
     CDS             complement(6986..7855)
                     /locus_tag="LNC71_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6465842.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7951..9255)
                     /locus_tag="LNC71_000749"
     CDS             complement(7951..9255)
                     /locus_tag="LNC71_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6465843.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9252..9761)
                     /locus_tag="LNC71_000750"
     CDS             complement(9252..9761)
                     /locus_tag="LNC71_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465844.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9936..12071)
                     /locus_tag="LNC71_000751"
     CDS             complement(9936..12071)
                     /locus_tag="LNC71_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6465845.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12154..13809)
                     /locus_tag="LNC71_000752"
     CDS             complement(12154..13809)
                     /locus_tag="LNC71_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6465846.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13811..14125)
                     /locus_tag="LNC71_000753"
     CDS             complement(13811..14125)
                     /locus_tag="LNC71_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6465847.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14295..14555
                     /locus_tag="LNC71_000754"
     CDS             14295..14555
                     /locus_tag="LNC71_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBK6465848.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14569..14883
                     /locus_tag="LNC71_000755"
     CDS             14569..14883
                     /locus_tag="LNC71_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBK6465849.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15613..16167)
                     /locus_tag="LNC71_000756"
     CDS             complement(15613..16167)
                     /locus_tag="LNC71_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBK6465850.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16293..18222)
                     /locus_tag="LNC71_000757"
                     /pseudo
     CDS             complement(16293..18222)
                     /locus_tag="LNC71_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18528..19244)
                     /locus_tag="LNC71_000758"
     CDS             complement(18528..19244)
                     /locus_tag="LNC71_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK6465851.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19245..21068)
                     /gene="glpO"
                     /locus_tag="LNC71_000759"
     CDS             complement(19245..21068)
                     /gene="glpO"
                     /locus_tag="LNC71_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBK6465852.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21082..22578)
                     /gene="glpK"
                     /locus_tag="LNC71_000760"
     CDS             complement(21082..22578)
                     /gene="glpK"
                     /locus_tag="LNC71_000760"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBK6465853.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22714..24174)
                     /locus_tag="LNC71_000761"
     CDS             complement(22714..24174)
                     /locus_tag="LNC71_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6465854.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24164..25459)
                     /locus_tag="LNC71_000762"
     CDS             complement(24164..25459)
                     /locus_tag="LNC71_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6465855.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25791..26468)
                     /locus_tag="LNC71_000763"
     CDS             complement(25791..26468)
                     /locus_tag="LNC71_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBK6465856.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26498..27589)
                     /locus_tag="LNC71_000764"
     CDS             complement(26498..27589)
                     /locus_tag="LNC71_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBK6465857.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27690..28124)
                     /locus_tag="LNC71_000765"
     CDS             complement(27690..28124)
                     /locus_tag="LNC71_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6465858.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28708..30165)
                     /gene="ascB"
                     /locus_tag="LNC71_000766"
     CDS             complement(28708..30165)
                     /gene="ascB"
                     /locus_tag="LNC71_000766"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6465859.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30162..32057)
                     /locus_tag="LNC71_000767"
     CDS             complement(30162..32057)
                     /locus_tag="LNC71_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6465860.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32115..33890)
                     /locus_tag="LNC71_000768"
     CDS             complement(32115..33890)
                     /locus_tag="LNC71_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK6465861.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33940..35436)
                     /locus_tag="LNC71_000769"
     CDS             complement(33940..35436)
                     /locus_tag="LNC71_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6465862.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35630..37252)
                     /locus_tag="LNC71_000770"
     CDS             complement(35630..37252)
                     /locus_tag="LNC71_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK6465863.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37580..38893)
                     /locus_tag="LNC71_000771"
     CDS             complement(37580..38893)
                     /locus_tag="LNC71_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK6465864.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39138..39362)
                     /locus_tag="LNC71_000772"
     CDS             complement(39138..39362)
                     /locus_tag="LNC71_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465865.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39382..39804)
                     /locus_tag="LNC71_000773"
     CDS             complement(39382..39804)
                     /locus_tag="LNC71_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBK6465866.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39946..40656)
                     /locus_tag="LNC71_000774"
     CDS             complement(39946..40656)
                     /locus_tag="LNC71_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465867.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40674..41171)
                     /locus_tag="LNC71_000775"
     CDS             complement(40674..41171)
                     /locus_tag="LNC71_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6465868.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41208..41660)
                     /locus_tag="LNC71_000776"
     CDS             complement(41208..41660)
                     /locus_tag="LNC71_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6465869.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41795..42400)
                     /locus_tag="LNC71_000777"
     CDS             complement(41795..42400)
                     /locus_tag="LNC71_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6465870.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42850..43470)
                     /locus_tag="LNC71_000778"
     CDS             complement(42850..43470)
                     /locus_tag="LNC71_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK6465871.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43472..44341)
                     /locus_tag="LNC71_000779"
     CDS             complement(43472..44341)
                     /locus_tag="LNC71_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBK6465872.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44364..44771)
                     /locus_tag="LNC71_000780"
     CDS             complement(44364..44771)
                     /locus_tag="LNC71_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBK6465873.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44790..46079)
                     /locus_tag="LNC71_000781"
     CDS             complement(44790..46079)
                     /locus_tag="LNC71_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6465874.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12930 a         9839 c         7890 g        15421 t
ORIGIN      
        1 actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt tatggcagaa
       61 atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc
      121 tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa
      181 tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac ggcaacgatt
      241 tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc ttatgttcca
      301 ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct cttcatgaag
      361 cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat
      421 cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct
      481 gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg tttagatcct
      541 ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg caaatccatc
      601 ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt cgtgatcata
      661 cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg
      721 accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa cggtgtccat
      781 gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg
      841 attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc accgactctt
      901 gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac tgataaattt
      961 tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa gatcctgata
     1021 tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga agaattgact
     1081 tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat gattgcaatg
     1141 gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat gtatgcaaat
     1201 gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca cgtggtgagc
     1261 caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt tcgagtcgga
     1321 cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt gcttttagca
     1381 atatcgatca gctgtacgat gatttttttc caagcggatt cgatcactgc agaaggtgct
     1441 tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt tgcactgttt
     1501 gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc gtttttaggt
     1561 ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc gatgtggaat
     1621 cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat tcctttgctt
     1681 tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga ggaacatcga
     1741 gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt
     1801 ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag ttggcttatc
     1861 acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca acagttttta
     1921 gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat tgaaaaaaac
     1981 tttacaagca cgatcatggt agtcttgatt tgtctagctg ctattttacc aattgctagc
     2041 atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt cgcaacaaaa
     2101 atttctagga accaacttta ttggaccaat atgttactag ctattctatt tgctatattg
     2161 gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat gaaggatgcg
     2221 aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc tgccgtttta
     2281 gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg taaacttgct
     2341 tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt ggatttacca
     2401 gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa cgattctttt
     2461 gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt ccttggttct
     2521 attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta caatttcatg
     2581 ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt atttacaagt
     2641 cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt acataaccat
     2701 tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg ataaatgcag
     2761 caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt
     2821 caagcagata gacagcgact cgttcttcta ctttttccat catcaaaaaa gttgtctgct
     2881 gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta agggcgggat
     2941 aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc gttttttcta
     3001 gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct ccctcatatc
     3061 cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat tgatacactt
     3121 tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca agaatctgtt
     3181 ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta
     3241 ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc ataatctctc
     3301 ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc aactgataat
     3361 tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag agtggatttc
     3421 tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat tcctatcaat
     3481 tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa gccgagagta
     3541 aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc gcacctagct
     3601 tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca acgtgttcga
     3661 tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc atcgcttttt
     3721 tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag tggtcgtcaa
     3781 gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt ttgctccact
     3841 ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt tcttcgcttt
     3901 tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa actgctagac
     3961 aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat
     4021 tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata tcaggcaaaa
     4081 gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta tattctttct
     4141 tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat gcgtagattg
     4201 gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct ctttccgtta
     4261 tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga aaaagctgtt
     4321 ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac cgcagtttta
     4381 ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg cactagaagc
     4441 agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc gtcctttttt
     4501 agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg ttaaattccc
     4561 tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat cgtcagcttc
     4621 tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc ctaatttttc
     4681 ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga atgcagatcc
     4741 gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga atactttgaa
     4801 tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag ctgaaacccc
     4861 tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat cactgagata
     4921 ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac
     4981 ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa tcgtctgtac
     5041 attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag
     5101 accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta cattgacgcc
     5161 tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca tcaaaggcgt
     5221 cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt aatcatctaa
     5281 aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt ctcgacgata
     5341 gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta gttcgaatcc
     5401 tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg tcttagttgc
     5461 ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc
     5521 atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc cagcagtata
     5581 cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt caatatcgtc
     5641 catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat aattcttaac
     5701 gatcagcaaa actccttttc cactatcggc ttccttaata gccgtctgaa tctgatctgg
     5761 agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt cccctacaaa
     5821 cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa cttgttccgc
     5881 tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt ctggatagct
     5941 ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca tgattttttt
     6001 caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt tgctgagtat
     6061 catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa ggcgcatcca
     6121 ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt tccgagttca
     6181 ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt gattcattga
     6241 ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg ccgagagaaa
     6301 aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata ccatctgtta
     6361 ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc ctttctttta
     6421 catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat ataataaatt
     6481 tctaattttg ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga
     6541 gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc
     6601 ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga
     6661 ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg
     6721 tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc
     6781 gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt
     6841 cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaataacta
     6901 aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta acttcgaggt
     6961 actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg taagctgctg
     7021 cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt tcatttgttt
     7081 ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact gtttctgtcg
     7141 tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt attcgttgga
     7201 aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc
     7261 tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt tgacttagct
     7321 ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt gcttgttgga
     7381 tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg gcaagctttt
     7441 tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa tcttgatcga
     7501 ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct tcgagttctt
     7561 ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta gaactattta
     7621 ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt acattggaag
     7681 tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatggtt gttgaaggtt
     7741 ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag aaaatactgg
     7801 aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt cccatcctta
     7861 ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca taaacacaac
     7921 aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg ggcttttcca
     7981 tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt
     8041 tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa
     8101 ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc ctgcgaccat
     8161 ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt cgtagtttcc
     8221 ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac aaaagaatat
     8281 tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa tatgtttaaa
     8341 aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac cttttgccca
     8401 acgttttctg tgtcgatata gcatcttcaa cgtcacaggt acttccatga aaaagataat
     8461 ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa tactgatgtc
     8521 ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt ttcgatacat
     8581 cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg ttcgtttaat
     8641 gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt tggtacgatt
     8701 ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc gaatgaaata
     8761 attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat tgcttaaaat
     8821 caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc ctttattttt
     8881 ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta atatttctgg
     8941 tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat aattcaattt
     9001 cgtcatcaaa tactcgattg tatattcgat cacaatttct tcattatggg ctggcaccat
     9061 gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt gcttatgttt
     9121 gtataaaaac acataacacc agacaccaat aaaccataca aatccgccta aaatcggata
     9181 agaaacgaga aaaaaataca taatagacaa acaagtatct aagaataaat tacctgtatg
     9241 ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc aatgaatgtc
     9301 gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct ctcttctttt
     9361 gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt caatttttca
     9421 tcttcatatt caaaagattt atttactttt ttgtatagat ttcgattgtt ccacctagtc
     9481 aatacaaatg aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc gattaagaaa
     9541 tacagcattt tcttttctga taaaaaagcg tacaaggaaa aatccttggc tctttctggt
     9601 ctcgttaatg ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca tcctaaaaga
     9661 gccactaacg tttgccaaat ctttaaccag taattacctt ttgaaaaata cttatctttc
     9721 accaaaattg atgcaacttc ttctcttgtt acttttttca tatcctcacc tcacgttttt
     9781 ttgttacgac gtaggagttt accatacatt tcatttcttt tcacataaag tgttcctaaa
     9841 tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata ttttgttctt
     9901 tttatcagtt gttcttttta ttttttatct cttaattatg ttgctctttc caatacgaca
     9961 atgcttttca ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct
    10021 ccatcgtttt caaatggata taagattaca tgatcaaatt ccagtccttt tgctagactc
    10081 aaaggaatga tttggcagtt ttctaatcca tatgaatttt ttttaatcga ttcttgtagc
    10141 gtttgggttt cctcttctgt ttgggtgata actgccgctt tttctccttt tagagaaacc
    10201 aaaatctcag ctagtatttt caacaattct tcagaggatc gaaaagagcg gtattcgggt
    10261 tcttttcctt gcggtcggat cgatacgatg tcgatcttct cttgatccac agcataggtt
    10321 ttgaaaagct ctgtgattgc tccgctagaa cggtaactat ttctaagatc ataccgatgg
    10381 atcggcagat ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt ggaagaatta
    10441 aagattgcct gattctcgtc acctactaaa gtaaagtgag tttttggaaa taattcgatc
    10501 aatagaccta attgtgccag ggtataatct tgtacttcat caatcaatag atagcgaaat
    10561 gctggtacat ttaccttttc aacaagtaaa tgacgaatag ctaatataat gactgcttca
    10621 tcaacagtac gtgtgggttg ccaagcataa gctgagccat atcgtttttc ataaattctt
    10681 tcaaacaact gatgctcatc cacccatgcc atttcttcga tttgtctggt tatttttcga
    10741 tatttttttt gtaaaagttt ccttgcataa gctacaatac tttgttccga atcatcagaa
    10801 atcaatttac cgaataattc caactgccta tcttctgtca acgagagtac ctgattctgc
    10861 acagcaggac tttttgcttg tttcaaaaga tgattcttcc attcgctcat cagtgccttt
    10921 ttcgttgctt gaagcctatc gatcaattta ggatgagcag gaactttctc atatattttt
    10981 tcgatcgttt ttttagaaag gactgttttt ccatttttag taagtccacg gataaaattc
    11041 ggatgatcta aaaacaaagg atctgattgt tttagattat cgataaactt tttgctacgc
    11101 aaacgttctg tctgctccga tacattttct cccgtgatac gtttgaaata agcctcttca
    11161 ccttctattt cctctgctga aagctgtgaa accagctgta tgatggttag attcagtgga
    11221 tttctttcac ctaatgatgg taggacattc gaaatatagt cgataaaccg atgattaggt
    11281 gatagaatca gcaagtcatc ggatgtcatc gtatctcgat ataaatagag cagatacgca
    11341 atacgctgca tgatagcaga ggttttacca cttcctgcta cgccatttac caagatattt
    11401 tgacttttca catcccgaat gattgtgttt tgttccgatt ggatcgtagc agtgatagcc
    11461 ttcatttcat tggtctcatc ttggcctaag acttctaata atacgtcatc ttgaatagct
    11521 acagacgtat caaaaaactt gaccaaacga tctctttcca aaagaaattg tcgtctttct
    11581 ttaataaccg tatcgatttt attttgacga accgtataag aagatggacc aatcatgtta
    11641 ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa caagattttc atctgcatca
    11701 tctgtgaaat ttgctgtacc gatataaaag gattcttttg gctcattatc cagaaagtct
    11761 acaacgactt ttccaaagta gggacttttc aataaacgtt cgatctttgc tagctgattt
    11821 tctgcgtttt tctgtttgat attcatctga tcgatttctc tatttttcat ttcaatcatc
    11881 gaaaaagtat ccaggttatc taagtaactg tcaaaattca atcgaagctc gccagccatt
    11941 tctttcaacg actggtttcc ttctattttt gccgtatcca tcgattcccg cagttcgttt
    12001 tttttatcaa ttagcttttg atacacgttc ttcagatatg cttcttctaa ctgtttttgt
    12061 gtgttgacca tacacattct cctctttttt taaagtggtg gaaccgatta gtttacctct
    12121 tttaagagtc gaatgcaagc tttttaacga gtatcatttg ttaggatata tcagttcttc
    12181 aggtcggaca gtttgatata aagaaaatgc accatcaagg ttttttacgt ggtagccaga
    12241 ctgcttcaag attctttccg ctagatagct acgtaatccg ctatgacagc tgacgatata
    12301 ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt tcattcaaag ggatatgttt
    12361 ggcttccttg aatttgccct tttccaattc ttctgctgtt cgcacatcta ataaaatttt
    12421 tccttttgct agttctgtcg gcaattcatg ccattgtacg gtttcactaa gcccttctgc
    12481 aatgttcatt gctgcatatc ctagcatgtt tactggatct ttggctgagc caaatggcgg
    12541 tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt
    12601 tgctaaaata tcgatccgtt tatccacacc ttttgcccca acaccttgtg caccataaat
    12661 ctcgccagtc tctggatgga agatcaactt cagtaaaata tctgttgctt ctggataata
    12721 gcttgcatga tcttttcctg atacatgaat gacttcaaaa gacagttcgt tttctttggc
    12781 tgttctttca cttaaaccgg tggatgcggc agtcattcca aacgttcgta taattgctgt
    12841 tccaataccg ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc
    12901 ttgacgattg gctggtgaag caagagaaat aagtgcatct tttccagtga tttgctgttt
    12961 tacaacgata gcatccccca ccgcataaat atcaggaata ttcgtttgat aacgttcgtc
    13021 tactaggata cccccgcgta tgcctaattc gattccagcg gtcactgcta gctggttatc
    13081 aggacgaaca ccaatggata aaataactaa atcagcaggg atggtacgcc catcatccag
    13141 tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa tcacttctac
    13201 tccttgatga gacaattctt tttctataaa agctgccatt tcttcatcca gtgttggaag
    13261 aatatgtggt gcttgttcga tcaccatgac ttctaaacct cttcttttta ggttttctgc
    13321 catttccaat ccgatgaatc ctgcaccaac gatcacggca cgctttggct gtttttctaa
    13381 tgcatgcatc actttatcga tatctggtac atttctaata gaaaaaacat tgtcggcttc
    13441 tgttatccct ggaatcgatg gaacaactgg ttttgcacca ggagataaaa ccaatgcatc
    13501 gtactgttca atgtgttttt gaccctcatg tacgacttct accgttttgt tttcgggaaa
    13561 gatggctgtc acttcatgat ttggacgcac atctaaacaa aaacgagcgg ataatgattc
    13621 tggcgtttgg acaagaagat tttcccgttc actgatttct cccgaaagat aataaggcaa
    13681 gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa acaacgattt ctgcatcttc
    13741 cattaatcgg cgaagccgtg tagcagcaga cattccacct gctacacctc cgacaataac
    13801 gattttcatc ttatttccct cctactgttc gatcaaacca ttgattcatg ccaccacgta
    13861 cattcactac atcataacca gatttcttta attctttagc tgcttgttta ctcctcatcc
    13921 ctgattgaca aatcacatag acggtctttt ctttgtctcc atcatatctg tttatacttt
    13981 gtaatggaac atttttagct ccttttatat gcccgccccg gtattctgca ggtgttctaa
    14041 catcaagtaa cgctggattt tccaaaagct tttcctttaa ttctgggact gttatcgatt
    14101 ttattgattg aaatagcaaa ttcattttct gcacctcatt tacattttta taaatacctc
    14161 atggggtata ttaaacagga gctttattaa tatgtcaacc agaatgactc ttcatttttt
    14221 tcactacttt ttatatttgc ataaaatacc gataaggggt attatctaat tataaaaata
    14281 gaggagaatg tgttatggag attgataata aaaatatcct gaaccgtttg aaacgtacag
    14341 agggacaaat tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt atggatgtca
    14401 tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg atcgttgcag
    14461 ataatctgaa aaattgcttt gagaaacctg atagtaatcc agaagaacaa gcggctaaac
    14521 tagaacaagc gatcaagatg attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt
    14581 ttccatattg atgaattaga aaaatggtca gaaacaggga aaaacgtaaa aaacctgata
    14641 aaagcttctc cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat taccggttat
    14701 ctagactctg ccaatgcaga attcttagat ttgcaaggag tcgtttttca cgcttgcgcc
    14761 aatgctatgc gtgctaatca tatttcagaa agttctttac ctgaacaagt aatcgttgta
    14821 ccagcaggtg tccttgattt agttgaactg caatcccaag gttatgctta tatcaagcct
    14881 tagtcagtag aggttgcgac agaagtgatc agcttcaaga aatttcggct tatttttcga
    14941 agaagctact tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt
    15001 aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttgg gcttattacc
    15061 gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc ggtttgacct
    15121 aagtattaga gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat
    15181 accgaaggtc agctgattga acaccgttta aggaagagat tgtgaaaaag tcgcttagtt
    15241 tcgagtatta agaaaaattt tgtgaaaatg gatttctaca tttttgcata aatttggctt
    15301 aataccgaga aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt
    15361 gacctgagta ttagagaaac aaacggaaaa atagctgctc atattttgcc gttttttgag
    15421 ctaataccga aggtcagctg attgaacacc gtttaccaag aggttgtgag cctaccattt
    15481 aactccgaat cacaagaaat aatttcaaaa acaacttctc ctgtttttgg ttcggttggc
    15541 ttattatcga gaagtagcta ctgggagtct atttatcaaa aaaaggaatg cgacataggt
    15601 cacacccctt ttttattttc tttgaaacat ctttcggtac tcgacagcat acaagacaaa
    15661 agattgtaaa agaccgtaag tgatgacggc aaaaatgact ctcacccaca gcgttggtaa
    15721 ttctgggaaa agcagcaaaa ggatcatgcc aacatagatg acagcagtcg ctactttgcc
    15781 ataccacttc gctccactta atttgatatt tttagatagc ccgattaatc cagctacaaa
    15841 cataaagcct tcttttatca agaatagacc aaacaacagc caaagccacg gtcgatacca
    15901 tgccatagac aaaatcagaa aaagttgtgt caatttatct gcaaacggat caagcacctt
    15961 ccctaagtct gttacctgat tatatctccg agcaatgacc ccgtctagat aatcagacaa
    16021 acctgagaca acaatgaccg ctcctgctat ataaaatgac tgtctcagca caaaataaag
    16081 ataaatcggc agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg tttgtatttc
    16141 tttacgccag tctgtgctta atttcatttt atcgccctac ctcctcagca ctcttttctt
    16201 tttattttag cttacacggg gaataaaacc aatgatttca ctgtttttat ttttctttac
    16261 agacactgat attatgagct aatttgagat aatcacttgc tgttttcatc gtttactctt
    16321 ctttcttcct gtcgtttgcg catttctttt actagtactt tatgagcttt ttttgatcct
    16381 cgctctaggt cacgctgccg tttacgtgtt ttataatcga taaacagact gtctaaatcg
    16441 taacctgtcg ggtacagatc ttctgctgaa cgcaccagac gaacacgctg acgaggcacg
    16501 tggattattt ttttgtctaa atatacatca atcgtatctt cacctatatc ttcataaact
    16561 aacgcttctt tttggtattc attgacaaat atccgatcac cttttgcaaa gagctgacgt
    16621 tcttttttat tctccttcct atttgtaact gctgtcttag gaagaaattc ttttttctgt
    16681 gtccgatagg ctttttccgc caagtaacgc tctgcttgat ggatcaattt catcgacata
    16741 tccattttat gagcaatcca caaagcttgg ctttctcctg tttcttccaa cccgcaactg
    16801 atatttagga cgaagtgctt cccgatcgaa agccatagca gccggcacaa aatcttcatg
    16861 atctccggcg aatttcttga tttctccata atgggtcgtg gcaatgatca atgccccttg
    16921 ctcatacata gattccatga ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc
    16981 actgccaatc tcatctaaaa gtaccaacgt attccttttt atctttttca aaatagcagc
    17041 gatgttttgc atatgaccag aaaatgtact caacgcattt tctaaatttt ggtggtcacc
    17101 gacatcgacg aaaatctcat caaagacagc aagctctgtc ccttcttttg ccggaacttg
    17161 cataccgaac atcgccatca aagtcaataa accgactgtc ttcaacacga ctgttttgcc
    17221 tccggcattt gctccggtga taatcaagcc tcgatagtct ttaccgattt caacatccaa
    17281 tggtaccgca tgctcaggta aaaatggatg tctgccttgc ttgataatga tatgttccga
    17341 tttattgatt ctaggtgtgc ttccattgat ttctcggcta aattttgccc gagcaaagat
    17401 gatatctaac accgtgatcg tttcgatcaa taaatcgatc acttcttcgt tttctgcgat
    17461 agcaccagtc aatgccgcca ggacttgata ttcttcactg atttcttcag cttttaacaa
    17521 ctgatagtgt tcattcaatt tagaaatcac agttggttca ataaaaaccg tcgttctttt
    17581 atttgattca tcgataatcg ttccatctat tttattttta tagctggctt tgataggaat
    17641 cgtatagtat tcgccttttt gaacaatcat tgcttcttgg atcatttctt tgtttttcgg
    17701 atgacgcaag aatttcagta attttgactg gatttctttt tcgattagtt gtaattgttt
    17761 gcgtacttta cgcaaattac gtgacgcgtc gtcactgact ttattatttt ttattttctg
    17821 gtcaatcaac tcttctacat ttatcaaatc aggaagatgt ttgctataag caaacaacaa
    17881 gggtgtctgg tattgattct tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa
    17941 atccgcatat tcgatcaaat cggttggctg cagaatcaac ccttttttga cttgttctgt
    18001 cagtgcatcg atcctcggca accccataaa tggaacatgc tgactgcttt ctaagatcaa
    18061 gcgtgcttct cttgtttctt cttgacgatc caagacagtt tgtaggtttg tcgagaccgt
    18121 catatcgctg attcgttttt tgctatagtt tccaattgct cttgcttgga tttccttttt
    18181 gatccgatca aattgtaatt gactaatcat ttcgttgtcc atattctctc cttttcttga
    18241 agagatacct gtcccaaaat aaataagggc ctagagataa ataaaaggat agaacacagc
    18301 ttaaaaaagt catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac
    18361 cgtttcataa gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata
    18421 ccagctacac tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact
    18481 atagcaagac acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat
    18541 gaatagacac ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa
    18601 tatgcccaat ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga
    18661 ccaaagtccc gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg
    18721 gctaagatca atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca
    18781 aaagctagca acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct
    18841 ggataattcc gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc
    18901 gttgcattcc aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct
    18961 aaaacttgcg caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc
    19021 atagctagag agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca
    19081 gccattgtta cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct
    19141 ttacttttgt ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt
    19201 gtcccaataa actcccccat gatttgagtc atatcactgt tcatttattt ctcccccttt
    19261 agttcccgca gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc
    19321 tcctcttctg tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca
    19381 tcaagctgat ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg
    19441 gccaaaacca tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa
    19501 ccaggataag gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa
    19561 aaatcagcga tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct
    19621 aattctgtca ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat
    19681 tttttagagt cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc
    19741 aatcgcaaag ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc
    19801 agcaaaccat ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt
    19861 tcatcacggc tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg
    19921 ttttccttat attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat
    19981 ccattatctc caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct
    20041 gcccagcttg cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc
    20101 acatccaata gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg
    20161 taatccgtat ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc
    20221 cgcccatctt gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca
    20281 tctacaacta aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa
    20341 ttcaagtttc gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg
    20401 atttcgatca cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca
    20461 tcatataaaa agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc
    20521 tttttgatat tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc
    20581 gcgcctttta gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc
    20641 agcgtataat tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt
    20701 gctactttga ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa
    20761 agcattggat ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca
    20821 actgtgtcag ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca
    20881 agtttggtcg agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt
    20941 ttgattcctg atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg
    21001 atcaacagat ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt
    21061 gaaaacatat ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca
    21121 accgttgtaa agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc
    21181 ttcataaaat tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa
    21241 aaatgctgcc cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa
    21301 gatatctgtt tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa
    21361 tacaggaatc ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata
    21421 agcaatcgac tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc
    21481 aaacatagcc cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg
    21541 aacaacataa acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc
    21601 tttttgcaac atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat
    21661 acttccttct aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa
    21721 attatttttg gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc
    21781 ataagtattc ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg
    21841 gtctcctgcc atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata
    21901 accataaact tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa
    21961 atctaaaatc tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc
    22021 attcgagtaa tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc
    22081 gatcgttcca aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc
    22141 aagaatccag cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc
    22201 gtggaaaaat tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct
    22261 ggattgccaa acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt
    22321 tgtttcacgc tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc
    22381 gatgaacgct ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc
    22441 cacccaactt tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct
    22501 tcctttcttg tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat
    22561 atacgttgat tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact
    22621 cttatcatac tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt
    22681 ttgacagttt tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc
    22741 tattcttctt ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc
    22801 acactgtttt ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc
    22861 ataacttcca atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa
    22921 agcaatacga taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat
    22981 ataaaaatcg atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact
    23041 atcttcagct cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa
    23101 ctgtgttaag gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata
    23161 gacttcgatc tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc
    23221 tactgccttt tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc
    23281 ccgaatgtaa gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg
    23341 ataaaatatt tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt
    23401 ttcgtataca ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat
    23461 tgcttgataa agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt
    23521 acttttttca ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc
    23581 tgctgatgcc gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt
    23641 ttctggtgta ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc
    23701 ataataaaaa tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa
    23761 tccaaattcc tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact
    23821 gatattaaac gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac
    23881 gagcatctta aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac
    23941 atcttcgtcc aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc
    24001 catcattcgg ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag
    24061 ttggacatgc tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg
    24121 caaaatcagg atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag
    24181 tattcgccac ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct
    24241 catgttcttc taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa
    24301 gaaggtggaa catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat
    24361 caaatagaaa tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt
    24421 atcctgtttc aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc
    24481 ctaagaaata gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct
    24541 gtgagccact aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag
    24601 aaacatacat tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat
    24661 aaacgtcttt ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca
    24721 atgttaattg ttcttcccac atctgattgt cgggacgaga atgattagcc agcaaaacaa
    24781 gatcatagtt ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag
    24841 acacaacttg ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg
    24901 gttctgtcat ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat
    24961 ggatttgttt ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac
    25021 caataattgc aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta
    25081 tctttttaac tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg
    25141 aaccagtcgc tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt
    25201 ttttttcttt caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag
    25261 tcagttcttc tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata
    25321 tcaaattgaa cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt
    25381 cacttgttgg catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc
    25441 cgacaattac aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc
    25501 tcaatcagaa aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag
    25561 cagaatcaac cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg
    25621 gacctaattg aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag
    25681 aaaaaaactc gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac
    25741 gagttttagc gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt
    25801 aatgcttcaa caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga
    25861 agccgcccat caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct
    25921 ttttctttca aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc
    25981 tcttcttcat tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca
    26041 tctgtaactg gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt
    26101 cgatagattt gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt
    26161 ccgtggccac cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt
    26221 aaggtatttc ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt
    26281 tcatccatat tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca
    26341 aaatctgcat cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac
    26401 cacaaacctg taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat
    26461 actttcattt tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga
    26521 aattatataa tgggcttggc aaataaagct ctttatatgc tttttcaaaa ggtttcgtta
    26581 caaattcatc ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt
    26641 gctgtccaat tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc
    26701 ggatcccacc gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa
    26761 gaatcgattt atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa
    26821 catgcagata atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa
    26881 aacggatacc tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt
    26941 ttttagcata ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat
    27001 aacgattttc caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa
    27061 attgttggat caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa
    27121 tcgctctacg tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca
    27181 ttgctcgtgg tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct
    27241 gttctccaga taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg
    27301 cttttgcgcc ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat
    27361 catgagcgat gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac
    27421 cagtaataac agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg
    27481 tgatcactcc attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct
    27541 tcaattgaac tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc
    27601 ccataattcg ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt
    27661 gtacatgcaa ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca
    27721 ttcattgtct gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga
    27781 tgaagttctt cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga
    27841 taaactaatg acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt
    27901 tcaacaaaac gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt
    27961 ggtattccat ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct
    28021 gtccttctaa acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta
    28081 aagaaaagac attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg
    28141 gcgtacatat catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct
    28201 cctctgtcta gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg
    28261 acaaaactcc ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag
    28321 tatgaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac
    28381 cgaaaaactg gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc
    28441 aagtaataag aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat
    28501 gccgatggtc agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc
    28561 cgagtcacaa ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg
    28621 tgatcgagga gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact
    28681 tttgtcacag tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg
    28741 tctgtaccaa tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc
    28801 ttgatcaaca tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat
    28861 atcaatgcag ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt
    28921 catctgctca atatgctgct tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc
    28981 ttcttctact ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata
    29041 cctatcatat aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca
    29101 ttcacttgat ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc
    29161 ttcccaattt tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa
    29221 atcaacaggg taggctgcta agatttcttc atcacctact tctattttta agttgacacc
    29281 tttttcttca aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact
    29341 aaagaacaaa gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga
    29401 atacggaaca agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc
    29461 atgacaagct tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt
    29521 cgcttgttcc aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg
    29581 ttttacacag ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt
    29641 gaatactgtt tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc
    29701 atattctgta accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat
    29761 cccatatttt agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg
    29821 tgtttcttca tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt
    29881 aaaacccatt tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc
    29941 gtgtctttta ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg
    30001 cgtcatcata tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata
    30061 cacatcagac acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc
    30121 cgctgtcgct ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa
    30181 cttgctagtt ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta
    30241 ctactatttg taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc
    30301 aaatctactt ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt
    30361 tgaaagtatt tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct
    30421 ccttcttcac tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg
    30481 tttacagaac tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat
    30541 ccactagcaa atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga
    30601 gtacttaaca ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga
    30661 aaagttagca tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat
    30721 ccttcaccaa tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg
    30781 gctcctgcgg caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat
    30841 ggtcgtttat atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct
    30901 gtgataaatg cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc
    30961 ccaagtactg ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca
    31021 cgaccgtaga aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa
    31081 atcactaaca cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta
    31141 taaacagcta aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca
    31201 actaataaaa taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga
    31261 atactttcgt gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct
    31321 aaaattgctg gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat
    31381 cgcaaggtta tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca
    31441 ataaccatag cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat
    31501 ataggcaaga aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat
    31561 tcagtagtta ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt
    31621 cctgccaaga cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt
    31681 aaaatgcctt ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt
    31741 tgtttggata atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt
    31801 cctccagctt cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc
    31861 ttcttattat cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca
    31921 ttttcttcgc ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt
    31981 acttccccct tgttaattaa tgccgtagca atccttctta caagataagt atagttctta
    32041 gaaaacggtt tctccattca attagtaccc cttctaatag cgaacgaaac tgtacaatag
    32101 tgtgtatggt ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg
    32161 tttctcttat agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata
    32221 agtggtatct caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag
    32281 cacaacaaag tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt
    32341 tgctgaatgg aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga
    32401 tcaagcagtt ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga
    32461 taaatctccc aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac
    32521 tcaaaaataa gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat
    32581 tgatttttga aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt
    32641 tgttgaatct gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt
    32701 atctcccctt gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg
    32761 ctttcagcta aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa
    32821 atttctaaag gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg
    32881 gcatccatcc tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac
    32941 aataacgaaa caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc
    33001 ttctctattt ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga
    33061 ttttcaattg cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt
    33121 tttgtgaggg tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt
    33181 tcttctcttc gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct
    33241 gaaacaatat aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg
    33301 atattggaaa aaattgtact gatttgttga ggaaaccttg cgtgttggat caccgtttct
    33361 gttccaacaa gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct
    33421 tttgtcgacg aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt
    33481 tttatttgta atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata
    33541 ctgacatacc ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg
    33601 ctggaataga ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca
    33661 attgattcta aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag
    33721 atagcaagca tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca
    33781 gctaattctt cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt
    33841 tgtcgtttat ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa
    33901 cctctcaaat gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata
    33961 ttcaaggttt ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt
    34021 caatctccta aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg
    34081 cttcctcatt tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc
    34141 aatgatttgg caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata
    34201 ctcacaacgt tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat
    34261 gccattttca actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata
    34321 acgaaaccct agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa
    34381 gggattatcg aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata
    34441 gacagcgcta cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg
    34501 ttcgtctcca tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata
    34561 agctggataa tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg
    34621 cagtgaacca aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa
    34681 gctacacata atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg
    34741 ggcgttcgct agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc
    34801 aatgggttca agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat
    34861 acgaaaagca ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg
    34921 ttcaaagacg gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc
    34981 cccatactct cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc
    35041 gatatcatat cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt
    35101 tggcttctgt tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc
    35161 ttttaaacct aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat
    35221 cgcttcatga gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc
    35281 ttcattccac tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt
    35341 aggtaatttg ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc
    35401 ccataaaaaa ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca
    35461 atttcttact tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag
    35521 aagagaagaa aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca
    35581 taagacgatc cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt
    35641 ccaacacaaa tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag
    35701 gataatttga cacaataacc cttaaagctt cacgtggaat agtgaacgtc tgcttttcac
    35761 tggacatatt acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga
    35821 catgctcagg cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc
    35881 tacgcaaatc gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt
    35941 cttctgcatt gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa
    36001 acccagcatg ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg
    36061 ccttgattga ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca
    36121 tattgattgt ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc
    36181 ctaactcttc tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa
    36241 acatcttggc actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg
    36301 gaagatcgtg attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc
    36361 atttggataa tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt
    36421 tgccttcttg ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc
    36481 ttttaggatt ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag
    36541 tcatcagatt tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt
    36601 taggtccatt acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca
    36661 tacgaaagcc cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc
    36721 gaacctgttt atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat
    36781 attgccccgt tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat
    36841 taggctcttt tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt
    36901 tatttttacg ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat
    36961 ccatgatgat acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca
    37021 tcgtaccgaa ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat
    37081 tcggagactc gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat
    37141 ccaacctgga aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac
    37201 ttcttggata gatttgataa acaactgaat ggtgccacca tttttgttcc atgattttcc
    37261 tcctataaat ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa
    37321 aattgtaacg ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa
    37381 aacagctgag gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat
    37441 gctgatttca caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca
    37501 aaaaacggca aaatataaga aactattttt tcgttttttt ctctaatact caggccagaa
    37561 cgctgatttc acaacctctt tatttcacat tcaatatcca catgaatccc acaaatacaa
    37621 agaacagacc atacatgatt ggagatactt cttttcctct tttagctgct aacatggtaa
    37681 ttggatagaa gataaaacct aatgcaatcc catctgaaat actataagtc aaaggcatgc
    37741 cgatcaagat caagaatgaa gggatcgcaa tctctaagtt tccccattcg atttgtcgca
    37801 aagattgtgc catcaatact cctaccataa tcaatgctgg tgcagttact tgtgaagtaa
    37861 caactgatag taatggagaa aagaacaagc ctaacaagaa gaaaatccct gtagtaatag
    37921 ctgtgatacc tgaacgtcct ccaacggcga taccagctga tgattctaca aatgcaccaa
    37981 caggagaagt accaaataag gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt
    38041 tccctacacg aggcatcttg ttgtctttca tgaaacctgc ttggtttgct aatccaacta
    38101 aagttcctgc tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg acccacattt
    38161 gaagagaatt gatgtcccct acatgtttta atgcaactaa aaatgttggt ttcaaacttg
    38221 gtgctgcggc aacaatttca gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg
    38281 ttgtagctgc catcccgatg aagatgccac ctggtacacg ccgaacaagc aagatagcag
    38341 ttacgactaa tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat cctaatccta
    38401 ctaatgtaga ttcattcgaa acgatgatcc caccttcgct cagaccaagg aaagcgataa
    38461 atagaccgat accgccagaa atcgcatatt tcaaatctgc tgggatcgca tcaatgatca
    38521 tttcccgcag cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg
    38581 ctaatgctgt ttcccatgga atccccattc ctaaacaaac agaataagca aaaaaagcat
    38641 tgatcccaag agcaggtgcc gttccaattg gataacgggc taataccccc attaaaacac
    38701 agccgatcgc actggctaat gcagtcgcag taaacactgc tccttcatcc attccagaag
    38761 cacctaatac agttggattt acaaataaga tataagccat tgagataaat gttgtaaatc
    38821 ctgccagtac ctcacgtttt accgtggtgt tcaatttttc tatttcgaag taggaaagta
    38881 ttttttcctt catcttccat ccccctaaat ccgaacatta taaataaagt ccgttaaaat
    38941 ttcgatttat taataaaaca acgtgtttta tcatatacta aaacaaaaca taagtaaaca
    39001 aaaaattcgg attttaagca aaatccgaac gttccatctg attaaatccc tttatctcaa
    39061 agttcatgaa cattcacttt ggattacaag aatttcttta ttgtgttaat aaagtttctt
    39121 cattctagtc tcaaaaatca atagtgtgat ttgattttac catttcgttt ctgttcttct
    39181 ttgatgaatt cttttgtatc gacaggattc agattattta gtccgccaaa acctgtatcg
    39241 accatttttc cccagccttt tgtcttcttt tttcctgatt tgagattctt tgctcgaagc
    39301 ttctcactat gttttgctcg ccgatacttc aaataacctt ctaatcttcc cattagatcc
    39361 actgtttttc ctccttttta ttcaatcaga cgactcagct tttcctataa caaaataatt
    39421 gccttcaggg tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga tttctcctgg
    39481 ttcttccatg ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg
    39541 tggtcctaat acctctggtg aaaacttctg cacaaatact tttgggaata cgcctaattc
    39601 taattctttt gagatgctga gtacaatact cttaaattct tctggcaatt cgattgtttc
    39661 agaaatttct gcaccaattt tttctacaaa gaatcggcaa atcatctcta cgtcattgac
    39721 ataaagcatc acacgtgttt tttgtatcat catttgaccc ttctttctat ttgattttct
    39781 ttcctgtcta taaactgatt ccattatatc acgaaagcgc aaacaccttg tgtttgatac
    39841 tattcaagaa aaataaagca atagtttcta ggattgcttt cctccacaaa ctttttttac
    39901 aaaaaaaaaa cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc
    39961 tgattcatct ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt
    40021 ttccttcaac gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc
    40081 aatgaccagc accgctgctt gtgtttcttc cgtcagcatc gttctttggg cttcacgcca
    40141 gttgagacag cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc
    40201 cggagcatcg gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa
    40261 atgcagattt cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa
    40321 ggaaatacta ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt
    40381 tgcacgtttc agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt
    40441 aaatgcctga cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta
    40501 atttcttgct tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt
    40561 tgttacgtgg ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac
    40621 ttcccagaaa ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat
    40681 aattgcttga tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg
    40741 ctgtatttga taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa
    40801 acaccgagcc aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt
    40861 aaaaatgtac cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct
    40921 aaaaaatgat cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc
    40981 gcaggctctt gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct
    41041 gtaagttttt ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta
    41101 tcttttccaa atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct
    41161 gaaggcgtca tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt
    41221 ttattgcctt ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa
    41281 agatattcca attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag
    41341 tagttgtttt ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt
    41401 ttcttgattt ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat
    41461 ttcttgattg ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct
    41521 tggatgattc caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa
    41581 tcgacttctt tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg
    41641 tctcgaagga tttcagccat ttcatcccac ctttattttg tttgtaagac tagttcacct
    41701 ctttttgttg caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag
    41761 taaagtatat tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt
    41821 tttctaaaat aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt
    41881 ctatactaaa aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac
    41941 cgtggatcgc acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt
    42001 gtttattcgt atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt
    42061 catagacttc tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag
    42121 gaagtgacca ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga
    42181 tataggcacg aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt
    42241 ttatttcttc gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga
    42301 tctcctcaaa acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa
    42361 gtgcgagtag tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag
    42421 caaatgtatg actatgtaat caagtataga agactggttt aattacggca aataaatata
    42481 atgaaatctg caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt
    42541 ctctggggct gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca
    42601 aacgaaaaat atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc
    42661 tttttcacaa cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca
    42721 atattcatca aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa
    42781 cgacaggctc acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc
    42841 atttctttgt tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta
    42901 atccacttga gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc
    42961 atattttcgg tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt
    43021 cgttcgatct tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg
    43081 atctgattca cgatcacgtt tgtaccgacg ttgccataac acttcaaagt ctgctgttag
    43141 gcgaatcgtg atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg
    43201 cttatcacta aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc
    43261 aagtgctttg taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa
    43321 attgttaaac ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata
    43381 catatctgga aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact
    43441 accagccaga agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt
    43501 ccaagttcat aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg
    43561 gaatcacatt atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta
    43621 ctttcgtcca gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga
    43681 tcaattcagg ataatcttct tctttcacac ctgttcctcc atggataact agggggacat
    43741 caactactgc atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt
    43801 gtccatgatt cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt
    43861 tcactgcgtc ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt
    43921 ctgtaccgcc gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt
    43981 ctactactgc ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca
    44041 tgacagaagt atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt
    44101 ctaagtgcaa gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta
    44161 acacatgcat gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac
    44221 ccatttcttt tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag
    44281 ctccaactgt aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca
    44341 tcttttattg ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt
    44401 tgtcgcatct catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga
    44461 gcagcatctg cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc
    44521 gtctgtttat tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat
    44581 tcagaaaatt taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta
    44641 gtggaaatct cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc
    44701 gatagggctt tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa
    44761 aatccaaaca tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc
    44821 caggcatata agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgttgaat
    44881 cccatcgaat taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga
    44941 gcaataccgc caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct
    45001 gtccctccgg caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg
    45061 ggcaaagtaa acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca
    45121 gcatttgggc tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact
    45181 aatttcatag ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca
    45241 gctagcaata catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata
    45301 tagttttgat ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg
    45361 atatatagcg ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa
    45421 tgtgtcggca acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc
    45481 ttagcaacac cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca
    45541 tttgatccag tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa
    45601 caagctccga taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag
    45661 ccaaagactg tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac
    45721 ttccattttg tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt
    45781 gcaatatctc tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa
    45841 gggatgatcc cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt
    45901 ccaacgatga tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg
    45961 ccagagatga cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat
    46021 gcaatggtga tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg
//
LOCUS       DAFCHQ010000012        44533 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000012 DAFCHQ010000000
VERSION     DAFCHQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.012"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="LNC71_000782"
     CDS             complement(<1..132)
                     /locus_tag="LNC71_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK6465875.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..781
                     /locus_tag="LNC71_000783"
     CDS             542..781
                     /locus_tag="LNC71_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465876.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            848..1057
                     /locus_tag="LNC71_000784"
     CDS             848..1057
                     /locus_tag="LNC71_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465877.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            1117..1788
                     /locus_tag="LNC71_000785"
     CDS             1117..1788
                     /locus_tag="LNC71_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK6465878.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1841..3817
                     /locus_tag="LNC71_000786"
     CDS             1841..3817
                     /locus_tag="LNC71_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6465879.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3830..4585
                     /locus_tag="LNC71_000787"
     CDS             3830..4585
                     /locus_tag="LNC71_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6465880.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4582..5406
                     /locus_tag="LNC71_000788"
     CDS             4582..5406
                     /locus_tag="LNC71_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465881.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5707..7806
                     /locus_tag="LNC71_000789"
     CDS             5707..7806
                     /locus_tag="LNC71_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6465882.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7844..9901)
                     /locus_tag="LNC71_000790"
     CDS             complement(7844..9901)
                     /locus_tag="LNC71_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBK6465883.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(10015..10227)
                     /locus_tag="LNC71_000791"
     CDS             complement(10015..10227)
                     /locus_tag="LNC71_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465884.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            10414..10881
                     /locus_tag="LNC71_000792"
     CDS             10414..10881
                     /locus_tag="LNC71_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465885.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10878..11120
                     /locus_tag="LNC71_000793"
     CDS             10878..11120
                     /locus_tag="LNC71_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBK6465886.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            11138..11440
                     /locus_tag="LNC71_000794"
     CDS             11138..11440
                     /locus_tag="LNC71_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465887.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11512..13566
                     /locus_tag="LNC71_000795"
     CDS             11512..13566
                     /locus_tag="LNC71_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465888.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13566..16178
                     /locus_tag="LNC71_000796"
     CDS             13566..16178
                     /locus_tag="LNC71_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBK6465889.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            16175..18706
                     /locus_tag="LNC71_000797"
     CDS             16175..18706
                     /locus_tag="LNC71_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465890.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18758..19090
                     /locus_tag="LNC71_000798"
     CDS             18758..19090
                     /locus_tag="LNC71_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465891.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19091..19684
                     /locus_tag="LNC71_000799"
     CDS             19091..19684
                     /locus_tag="LNC71_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465892.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19847..21541
                     /locus_tag="LNC71_000800"
     CDS             19847..21541
                     /locus_tag="LNC71_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBK6465893.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            22229..24106
                     /gene="ltrA"
                     /locus_tag="LNC71_000801"
     CDS             22229..24106
                     /gene="ltrA"
                     /locus_tag="LNC71_000801"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK6465894.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            24237..24614
                     /locus_tag="LNC71_000802"
                     /pseudo
     CDS             24237..24614
                     /locus_tag="LNC71_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24614..25819
                     /locus_tag="LNC71_000803"
     CDS             24614..25819
                     /locus_tag="LNC71_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK6465895.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25835..26479
                     /locus_tag="LNC71_000804"
     CDS             25835..26479
                     /locus_tag="LNC71_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465896.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            26490..27296
                     /locus_tag="LNC71_000805"
     CDS             26490..27296
                     /locus_tag="LNC71_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465897.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            27318..27548
                     /locus_tag="LNC71_000806"
     CDS             27318..27548
                     /locus_tag="LNC71_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465898.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            28094..28369
                     /locus_tag="LNC71_000807"
     CDS             28094..28369
                     /locus_tag="LNC71_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465899.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            28411..28833
                     /locus_tag="LNC71_000808"
     CDS             28411..28833
                     /locus_tag="LNC71_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465900.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28852..30444
                     /locus_tag="LNC71_000809"
     CDS             28852..30444
                     /locus_tag="LNC71_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465901.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30456..30791
                     /locus_tag="LNC71_000810"
     CDS             30456..30791
                     /locus_tag="LNC71_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465902.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30938..31132
                     /locus_tag="LNC71_000811"
     CDS             30938..31132
                     /locus_tag="LNC71_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465903.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            31164..33536
                     /locus_tag="LNC71_000812"
     CDS             31164..33536
                     /locus_tag="LNC71_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465904.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33597..35057
                     /locus_tag="LNC71_000813"
     CDS             33597..35057
                     /locus_tag="LNC71_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBK6465905.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            35068..37272
                     /gene="topB"
                     /locus_tag="LNC71_000814"
     CDS             35068..37272
                     /gene="topB"
                     /locus_tag="LNC71_000814"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6465906.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            37288..37437
                     /locus_tag="LNC71_000815"
     CDS             37288..37437
                     /locus_tag="LNC71_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465907.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            37511..38104
                     /locus_tag="LNC71_000816"
     CDS             37511..38104
                     /locus_tag="LNC71_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBK6465908.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            38120..39019
                     /locus_tag="LNC71_000817"
     CDS             38120..39019
                     /locus_tag="LNC71_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBK6465909.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            39022..39507
                     /gene="ssb"
                     /locus_tag="LNC71_000818"
     CDS             39022..39507
                     /gene="ssb"
                     /locus_tag="LNC71_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6465910.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39873..40133
                     /locus_tag="LNC71_000819"
     CDS             39873..40133
                     /locus_tag="LNC71_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465911.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40574..40780
                     /locus_tag="LNC71_000820"
     CDS             40574..40780
                     /locus_tag="LNC71_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBK6465912.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40780..41031
                     /locus_tag="LNC71_000821"
     CDS             40780..41031
                     /locus_tag="LNC71_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465913.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            41046..41483
                     /locus_tag="LNC71_000822"
     CDS             41046..41483
                     /locus_tag="LNC71_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465914.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            41476..42183
                     /locus_tag="LNC71_000823"
     CDS             41476..42183
                     /locus_tag="LNC71_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465915.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42564..42743
                     /locus_tag="LNC71_000824"
     CDS             42564..42743
                     /locus_tag="LNC71_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465916.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            43084..43347
                     /locus_tag="LNC71_000825"
     CDS             43084..43347
                     /locus_tag="LNC71_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBK6465917.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            43341..43691
                     /locus_tag="LNC71_000826"
     CDS             43341..43691
                     /locus_tag="LNC71_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6465918.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43715..44329)
                     /locus_tag="LNC71_000827"
     CDS             complement(43715..44329)
                     /locus_tag="LNC71_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6465919.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT        16346 a         7069 c         9057 g        12061 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaatagta
      541 gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag gaaggtatgt
      601 acagctgtta gctcgtggtt attcacctaa agaagtattc aaagccttat ctaaaggaac
      661 agatatggaa aaagaaagat tgcgaaaaga attcgattat tggagaaccc ataatggaat
      721 caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa cccaagactg
      781 aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa ggaaggtagg
      841 aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaag aaatggaaga
      901 aaagttacta aaaacgatga agcaaaaaca ccttaaaaga ttatctgtcg cgcaatatat
      961 caatgatatg cagttaacag gaaaagagaa agcttgtttg ttaggttcaa tgaaaaactt
     1021 tgaacagttg agacgaactt atagaattca tttttaaaac tttttctatg gaaatgagaa
     1081 aaagtttttt tgttttgtct gaaaggaggc agatggatga aaaaatggtt atttggtttt
     1141 ttaggagtca ctttaatcgt cttttgcagt gtgtttggct atgttagcta ccaaaaacat
     1201 gaaggagagg tgtttaagca aaatatagaa aaaaagatgc ccgtagatca aatcaatgca
     1261 catgcaaaga gttataagga ggatgcaacg aacgtcaata atgatatgtc tttggggcaa
     1321 atgttgtcca tacaaaaaga agcaatcgaa atgggcgtca ataaacaagt atttgcacaa
     1381 attcagattc ctgcacttgg tttagcattg ccaattttta aaggggcaaa tcaatacact
     1441 ttgagtcttg gagcggctac ttatttttat gaagatgccg aaatgggcaa aggaaactat
     1501 gtcctagcag gtcataacat ggaaatgcca ggtgttttat tttctgatat tcaaaagttg
     1561 tcattagggg aggtgatgga cttagttagt aacgatggcg tctatcgtta caaggtaacg
     1621 cgtaaattta tcgtacctgg gtactttaaa ttaatcgatg gcgttcctga agagaactct
     1681 ttcttgtctt tgccaaagaa aggagagaaa cccttattaa cgttgtttac ttgtgtttat
     1741 acgtcacaag gaaaagaaag atacgtggta cagggagagc tacaataaca aggttttaat
     1801 caacgcgagt cgttgaaata tgaaatgagg agagtaaaaa atggcaataa atcggtatgt
     1861 aaaaaaagct ggaatgcttg ttggaatgtt aggaatcatt gcaggctgct ttcaggggta
     1921 tcaagtacat gcagaagatg tcacgcaaaa aacaccacca gagaaggtca atatcacggt
     1981 tcataaatta atgtacgatc aaggaacgca attgaatgtt gatattgacg ggatcaaaaa
     2041 cgatggttat acgcacgatc aatatcctac aggtgtaacg aaatacaaca aggcagatta
     2101 tggtgatgtt gagtttacgt taggaaatat tacggatcaa gtattaccga gagaagattc
     2161 tgatctaacc aacgcgaaag tggacgagat tgtacaagat gtagaagaca agggatctaa
     2221 cagtgagtac gtcaagaatg caaccaacaa aattacttca gctgtagatg aaaatggtga
     2281 gatcactttt actgatcaac cagcctatgt caattccaaa gggaatgtat atgtggttta
     2341 tgaaagtaaa tccgctgctg gattagtcac acaaaaagca aaaccaatgg ttgtgattgc
     2401 gccaatgaca gataaaacag gatcaggatt tttaaaaaat attcatttgt atcctaagaa
     2461 cattgtttct aagttgagtt ttgaattaac gaaatttggt gacgatggcg cagaccaaag
     2521 caaacaaacc cttttaaaag gggcaaaatt tgagttatac aagggcgaac ctggaaaagg
     2581 aacaaaattg ggtgatcttg tatcggatga tcaaggaaag ctgactgcga ctgatttgac
     2641 gttaggaaaa tactattttg tggaagttcc ttcggaagtt gtagtgggat cagatcaaga
     2701 accaacagca gatcaatatt tattaggcgc agacgctcgc aacgatgcgc acaataaatt
     2761 aacctttgaa attacgaatg atggcgtgac aagtgactta aaagcttcat acgtaaacta
     2821 taaagcacca gtgatcgata aaacagtaac aaatggaaca ggacaagaac attctttcca
     2881 aattggtgac gcagtaaatt atcaaggaac gattcatatt ccaacagaca ttgcaggtgg
     2941 cgcagacggt atcacagtaa acggtgtgaa aagcgaaact tctccttaca gtgtatttaa
     3001 atggggagat acagcaggta aaggactttc ttatgttgct gaaaaagcag atattaaagt
     3061 aacaaataat gatggttcag tcgtattaaa agaaaatact gactataaaa tcgaaaattc
     3121 tgatagtggt tttgtgatcg attttatcgt aaataatgga caagtaagtg atacagttgc
     3181 gaatttacat ggtcaagatc taaaaatgac ttataacatg tttgtgaatg atagtgctga
     3241 aatcgcaaat ccattaacaa atagcgtgga tttcgtttat aacaataatc catttaatca
     3301 ggaagaacac cacgaaaaaa cgaaagctga cgttgtgact tatggcgcaa aattcttaaa
     3361 agtcgatagc ggcctatttg gaacaggtat caaagcaaca ccgcttgaag gcgcagaatt
     3421 tgcagctaag aatgcagaag gtaagtacta cggtggactt actgatacag ataaagatgg
     3481 tgtcaaagaa gcagtttggg tagacgatgt agcaaatgca gcaatcctaa aatcagataa
     3541 agagggtcat tttgaaatca ctggtttaac tgaaggagat tactcactgg aagaaacaaa
     3601 agctcctgaa aactatcaaa aattaacaaa agagatctct ttcaaagtag acaaagattc
     3661 atacaaagaa gaaaaccgaa tcacgatcaa aaataatcaa aaagcttccg taccaatgac
     3721 tggtagcaac ggtttccaat cttacgtatt gatctcttgc ttgttgctag gagcaggggg
     3781 actttctgca gttgtttact ttaagaagaa agcctagaag gatcagctaa tgattattcg
     3841 taagccccca aaaagaagag tcatgcacaa actgatgata tttttttggt tgctgtcggc
     3901 agtagcagtc tatttaattc cagttgtgga aacaaatgta gcgaaatttc aacaacagca
     3961 aagaatttta gccattgaaa atcttgaaaa aacagattct tctttggatg ttgcgaaaat
     4021 agagttaggc gatcctgttg gtgttcttac aattccaagt atttctttaa aactaccaat
     4081 ttatgacgga acttccgaca aaattttaga gaatggtgta gggattacgg aaggaactgg
     4141 cgatattaca ggaggaaacg gtaaaaatcc tttaatcgcg gggcacagtg gactttataa
     4201 agataatctt ttcgatgatt tgccttcagt aaaaaaaggg caaaaattct ttattaaggt
     4261 aaatgaagaa caacatgcct atcaaattta tcgaattgaa gaagttaaag cagaagaatt
     4321 acaaaaaaat tatttagatt atcttatgcc tgaaaaagac caagatagga tcacgttaat
     4381 gacatgcaca ccaaaaggtg tcaactcaca ccgcttttta gtgtatggtg aacgagtttc
     4441 cttcagtaaa gaagatttaa aactacagaa aaaagcaaaa aataggaaat caatttcatt
     4501 ggattggcga ttagcactgc tattcgcttt tttttgcgtt cctattattt ttatttataa
     4561 aaaaataagg aggagatcgg aatgaataag agaaaatggc tgtttgtttg tgtagcagct
     4621 ctcttttcta ctgtagctta ttcgcaactg tctttcgctg acagcagtga tagtagtacc
     4681 actcaaacaa tagataattc aattactgca ccaagcgaaa cggacagttc ggagaatact
     4741 acagaagaaa cggcagattc aaatataagc tttactgaat caacgaatcc aacgtcagaa
     4801 agttcttcag agacttcaga aaagccgcaa gacgtagaag atacgcaagt gatcaagcaa
     4861 gccattcata ttcaaaaaat tatttcttcg aatcaaatca acaatcaagg cagtcaagta
     4921 gcccagcaag aaggtatcaa tggcgcgaag tttattgtct acgatatcac gaatatctta
     4981 gaagaaatgt cacaagatga taatgagaaa aatcaatctg tagaaataat tgagtcaaaa
     5041 ttgaaagatc gagcaaaaaa actttcatac gaccagttga aaaaagtggc tgaaggagaa
     5101 accaaaacga ttgatgatca ggaaggcgtg attgaatttt ccattgaagt acctgcaaac
     5161 aaaaaacaag catactatat cgttaatgaa tcaagccctg aaaacatttc aaacagtgaa
     5221 gatattgttt tattgacacc tgtttcaaat gaaaaaggtg aatttttgac ggatgtttgg
     5281 ttatacccta aatcagagaa atcagagccg aaagaagaaa ttaaaaaagt tgtgtcaact
     5341 ggtgtaaaga agaacttttt tgaaaattgt tgggaatttt ttgctcaatt attttttgat
     5401 aattaggatg aaatgatgaa aataataaga atattagttt ttaatctatc ctttttagcc
     5461 gcaacaatat tgtgttgcgg tttaattttt tctcaattat caatttatcc aagatatcaa
     5521 cgattgttca ttcattttct aaacattcaa tgtgcttcca tgccattaga ggtgattaaa
     5581 aagctcttga tcaatccaat ttttatggtt agtttggttc ttcttacagg attggtaacg
     5641 tgggtattag gaaaaaaaga atgaaaagct tacgatcgtt taaaaaaata ttagatggag
     5701 ggcattatgg aaaataaaat tcattgcact acgggaagag taattacttt agtgttgttt
     5761 ttgttccttc ttctttttgc gaggggaact gcagtccagg ccttagaaaa agttgatccg
     5821 agtcaaatca aagaaaataa taatctggtg ctttatcatg atagtgattc attgagaaat
     5881 atcgaagtaa ctgggatcaa tggatttaag atactagatg agtattcaac taaaaaacaa
     5941 aaagtacttg cttggccaaa agtcaaagat ttgaaaagta cgtcaaaaat cgttgcccat
     6001 tatcaaaaag taggtactta tctaggaaaa gaaattgatt gtgacgtgac tttttctaat
     6061 tttgtagcgc acgctggggt taaccaagac ggattagcta aaacaattaa tggcaaaaag
     6121 tattataatt atttgctctt tgataaaaat atttatcgag gatttttcat gcagtcaatc
     6181 aatgtcatga atgcaaaact agaatttaag tatgcaggaa caaaaacaaa agtagctttg
     6241 agtgaaccac agccagaaat tcaatcaaaa atgagatcga tgagagcagc tggtattgat
     6301 ttaaacgata catttctatc catttacagt ttaaatggac agtatcagca acgtgtgtct
     6361 tggtataatg acaataatga atctgtcggt gaacgtaata ttgatatgag agaatttgtt
     6421 gggtatgaaa aaatgggtag cttaccttat tttgtcacaa cagatacagc atgtgcagaa
     6481 tacaaagctc ctgcgttatc aacaaacaat ttaacttcaa aagtagtggg aggacgtgca
     6541 gaaaaggctt atagctcgaa tgatcatttc accgatgttg taggagctga tacttatcac
     6601 agaagtggtg taacgtatac gcttcaaggc gcttccccaa cattcatgat tggcgcaaat
     6661 acgaatagta tgatgtttag ctttgatact gcattgctat ggacaccaca accatcgaag
     6721 cctacaaaag aagtgtttaa caaagctaat actgaagagg cagcacacaa tattgacaaa
     6781 aaagtgattc cacaaggatc agatgtttac tatcatattc accaaaagtt tgatgcatta
     6841 acagtcaaca caatgaacaa atacaaatca tttaaaatca ctgatacctt tgacagcaaa
     6901 aattttgata tggtatcgga tgggaaaaac tatgatggcg cattgtggta tgttgaagcg
     6961 aatggtactt ggaaaaaagc tgatacagga ggaaagttaa ctgtatcagg taatacagtc
     7021 acttatactg cttctgacag ctttttagct aacgctgcaa attacggtag ggattatatt
     7081 ttgcagctcc atatgagagc aaaaaatgat gcaaaaggaa ttgcaccaaa tgtggctact
     7141 gtaacgttta acaatgatcc gaacaaagga acacaaaaaa caaatacagt agaaaactat
     7201 ttctctctta aaccaaataa aaaagtggaa caagatggga aagatgttga tggtcgaaat
     7261 gatggtaaaa aaggaacgcc tacagctcct ttaaatgcag gatctgaagt acaatatcta
     7321 gtcacacaaa aatggcatac aaaaggtgta gacattgcct cagatcatta caagcaattt
     7381 agtattcaag atccagtgga agatcgtttg acgtataaag aaggttctgc gcgagtgata
     7441 gatatgagta caggaaaaga tattacttca gaaggtacac taacctatga tagcaattca
     7501 agaacgctga agtgggaagc ttccgctgat ttcttaagta aaaatctttt agatggacga
     7561 gaaattcagc tgatatttac agctaaaact cctttgaatg aagaattgga tatcgacaac
     7621 caagcaaaag tttcagtaga aagtattgag aaagcaacta atgtagtaac gattggggta
     7681 aaacctaact tacctcagat cattgtgcca aaaactggat cgaatcattt agtgctaact
     7741 gttattggaa gtatattatt tcttacttta gcatcattgg gctatgtagt tttaaaactc
     7801 aaataaatat aaaaacaagg ctgagaatca gccttgtttt cttttatttg aagattggat
     7861 tcgcactaat aaatacatta tctacgccaa ttctaccagc agagtcaact acaaaattat
     7921 gacctaatcc gagaatactt tggttattat aaagtaaatt tgcattatgg gcagggctat
     7981 ttttccatcc taccatggca tcgatagcac catcgtctga taaaaggtca tcaatataca
     8041 gcccagtttg acttatgttt tctcccttag caccgtagca gcctaagctt tccaataaat
     8101 cttggaaagt accaccacca gggcgatagt gactgaagct gacttgtaat tctttagctc
     8161 gaatatctgc agcctgttga acaatatcat tgaattgcat tggggcaaca ccattttgtt
     8221 ggcgataatc atttaaccaa gcaagttctt tttgacgcat aaagttacgt aattcgtcta
     8281 atgaaatctt tttatatttg aatgtgattg ttttattttg atcgacgtta ttaatggatc
     8341 gttggccgtt tttatccacg gtaacggtag gatcgtcatt tctgcacaag aaataatttt
     8401 gatcagtcat gtttttccaa ggagtagcaa agctatcccc ttcgtatact gtttttgatt
     8461 cactagagag aacagcgttg gtatcagcat ttacatgatt aacagtcaca ttaaatttac
     8521 gagtgtagtt gaaaatataa ttttgacttt cagtcacatt gtttaaggtt actgaatttg
     8581 ttggttctac ccgatcgtct aaaacatatc cttgaataga aggagcagtt aaagtgacac
     8641 tgtcaccgtg agtgacattg actatctgtt ctttcaaaat attaactcgt ccttcgtttt
     8701 tgtagttgtt attgcacaac aaattgattt gatgcacttg attataattg aatgtgattg
     8761 ttgtattacc tgtgattgtt ttgctttgag tatcattacc aatcactttg tagctaaaat
     8821 tatctttagc gccttcgcta tatttaaatg cttttgcctt agcagtgtaa gtagaaccag
     8881 aagttacttt tgcttgatct gtagcaatca cttgacctgt ttgattgtca atatggttaa
     8941 tagtaaccgt atattctttt ggtgtttcag gtttgtgttg caacccttta aatgcagcat
     9001 caagactatt tttagcattg tttacttgtg tttgagaaac cttagaatcg tctagtactt
     9061 ttttagcatt ggataaagca gtctgaaaat tattccaaga ttcttctgta aaatctcctt
     9121 tagcagttga ttttactcgg ttgtataaag cttgtaattc agctttattc acagtaggaa
     9181 ctggtttttt ctttaatcct ttgtaagcag aatctaattt atttttggca ctatccactt
     9241 gcttttgagt cgcttttgag tcatttaaca ctttcttagc attgtttaaa gcggtttgga
     9301 atgcgttcca ggtattgtcg gtatagtcac cttttttcgt tcccttaacg ctcgtataaa
     9361 gagcttctag agccgattta ttcactttgg gtttagaggt gtcagcgcca taccaaccga
     9421 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
     9481 tgtagtgatg tgagccagat tttacaccag gaaggaaaag gcggtagatc ggtttattat
     9541 ttttttcggc agaacgccag gcaggccctt cataagtcca gccgtaattt ttagtcaacc
     9601 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aagaccttta ttgtacaaac
     9661 gatagacaag atggccaccg ctagtcggtg cttcccaggc ggggccttca taatttcccc
     9721 attttatctt tactagatgg tcacgttctt ttttgctaga ggtgtaaaaa tgttcattat
     9781 tatttggatt gtaaaggcga tacatgacgt tagttgaagc agcgtcaaca gatccagcaa
     9841 gactaagtga ggcagcaaat gttccaatag ctaaaacagt ttttggatta aataatttca
     9901 ttttttcaac tcctaaattt aattaaaaat gtttacatga ttattatata tcggctaacc
     9961 gatatagtca ataaaaaata agaaaagcct tatataattt tggcttttct tattttattt
    10021 gttagttgaa gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt
    10081 tatgaatctc atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa
    10141 gggtacgcca gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca
    10201 ttcgattttc tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata
    10261 gctctttttt gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt
    10321 ttatgagtat tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat
    10381 atactaaaaa taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc
    10441 tctatgtttg caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa
    10501 gctaaagaag ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt
    10561 cttattcata atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg
    10621 catccagatc ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga
    10681 tacaaaatag tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt
    10741 attgttgttc cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag
    10801 gatgagaagc gaacgaaaga gaaattaatt catgatggat cagagttaaa taaatacaaa
    10861 ttacaaaaga ctagtgagtg attgctagtc tttttattta tcctgaagga ggaatcacag
    10921 tggcaaaagt taaaacacca ttcgaaaaag ccgcgtctaa aatcttagaa cgccgtggtg
    10981 tttctgaaat acagtggaaa aaagaagttc ttgaaaaagc acaatttgac ctattacgag
    11041 gaaaagataa ggacttagaa gaatatatct atcaacgtga acgtgaaaaa cttgtattcg
    11101 atgaaattat taaacattaa aaggagcgaa taatcttatg tttgaatcaa ttacaatgaa
    11161 ttttttaact caaccgaaag tagtagctgc agttgatatt tttagagcat tacaaactgc
    11221 cgcaactagt ttgaaaaata atctgatgat ttttgcggct gtagctgccg ttgcctccat
    11281 tgctcttgga ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca
    11341 cgtaggttat gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc
    11401 aatgttctca atgtttggac aaaccccaca aggactataa aaaaaacaag taggaggagt
    11461 aaaaagtacg tggctacttt ttactccttt ttttatgaaa ggagaatatt catgtttttt
    11521 acgatttcag gaaatgcaaa attggataaa gagttaataa gaaaagcaac gaagaaagtg
    11581 ggaaagcgta cttccttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa
    11641 cagctacatg tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact
    11701 ctcacgttac atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc
    11761 ccactggatt atcaaatttc agaaggtttt tatcctacat tgatagattt aatccgcctt
    11821 gatgtgttta aagatacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa
    11881 ctgctacaag ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagtgagt
    11941 gaagtcgaag taaatgaaga agtaccatgg gaacgaccaa tctttccaaa gaaggagtca
    12001 aaagaaaaag agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata
    12061 tttgaggaag agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg
    12121 gaggatgaag agacggacga agtagaagtt gatccagttg aagctaatga agagtcaaca
    12181 tggactgaag gggattttca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa
    12241 aacgaaacag agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca
    12301 gagcatgaat ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat
    12361 caattacccc aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt
    12421 aaaggcacac ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca
    12481 caagaagatt tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat
    12541 gagtatgcga cggaacagtc gtctaattgg gagaaagtaa aagaggaact aaataaagaa
    12601 gaacagcgag tgattgaaga agaagtacaa aaagtcaaag agcggtacga aaagaaacgc
    12661 gacaatgctt attccttgtg tgtagaaaag atcaacaatt ttactaagac gaatcaagaa
    12721 gcgttatcga aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagag
    12781 ttctaccgcg gtttacgtac agataagcta aagattagca aagaattaaa tgaacaactg
    12841 aataagctat atcaaacaac gactgaagtt cttatggaaa aacacgaaca gatccagcaa
    12901 gaattacaag tacaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg
    12961 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
    13021 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
    13081 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
    13141 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
    13201 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
    13261 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    13321 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
    13381 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    13441 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa
    13501 ggatcaagcg attcaactgt acactcaaac acttccgaaa gttcagaatc aaatgaggtg
    13561 aaataatgcg tattagagaa acaacgagac gaaaagacaa acgattcaat ttagaaaaga
    13621 ataggtatct ttctcatttc tatcgctata ctccttcagg agaagagcta ccagaaaaac
    13681 atgacaaacg tatttctcaa aagatttttt atagtggctt agcttatctt cctttaaact
    13741 atgttgttac aagaatacca gaagcatttc atcgatttga gattattcca ggaagtttac
    13801 aaggagtgaa tatttttcag ttacaagaaa tgccgcagtt attccatttt gattcaactg
    13861 gtttttggaa tcctttgacc ccaacaacaa gtttaggcac acgtttagtt gtttctacga
    13921 tttgtgttgt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtccgctag
    13981 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    14041 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    14101 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    14161 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    14221 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    14281 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    14341 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    14401 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    14461 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    14521 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    14581 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    14641 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    14701 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    14761 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    14821 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    14881 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    14941 taggctataa aggatttagt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    15001 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    15061 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    15121 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggaaaaaagt aacttattag
    15181 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    15241 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    15301 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    15361 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    15421 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    15481 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    15541 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    15601 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    15661 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggagaa acgattgtac
    15721 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    15781 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    15841 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    15901 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    15961 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
    16021 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    16081 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    16141 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    16201 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    16261 gaaagtctta caagattatg ggaattatct gcagtatcaa gatgtattta gttggatttg
    16321 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    16381 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    16441 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    16501 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    16561 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
    16621 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggatcacc
    16681 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acgttactag caactgaccc
    16741 gaataaagta gaatccctac caacagataa aagaaatgct ttaacagcga aaaattttaa
    16801 aacagcgaat atcaatggga ttattactcc tgaacaagct aaaggtatgg caggtaatga
    16861 taaaagtggg ttatcagata tacaaaagga tcggttaaag gcattacaat accgattgga
    16921 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    16981 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattattt tagctggtga
    17041 atcttctttg gcagtagcct atttatttat cctatttacg atcgttactt gcattattga
    17101 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggtca
    17161 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    17221 tttagaatta cgaatctata ccttaatgct ttcaggtatc ggtgatttgc atgcggcagg
    17281 aaagatcaat ggattcttgt atgtcgttgc tttgattgta ctaaccattg cgctattcaa
    17341 aggctctcaa tcagttacga aaattttcgg cgttgataca agcctgaaaa acggcagtaa
    17401 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    17461 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    17521 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    17581 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    17641 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    17701 tgaagatctt acagaaaaag ccaaagacac cttagaagat aaattagacg gcacgagagc
    17761 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    17821 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc
    17881 agtgaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    17941 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    18001 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    18061 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    18121 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    18181 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    18241 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    18301 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    18361 agatagtcgt ttgaatcaat tcaaggataa tgatcaaagt tttgttcatc aatcacctgc
    18421 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    18481 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    18541 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    18601 atttactgca ccaacaaaaa taaatgtgac aagagaaaca aaaaacagga acaatgcatt
    18661 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    18721 aggccttttt tctatgtatg aaaaggagaa atcatttatg gataaatata gtaacatgaa
    18781 aaaccctcca agatttagtg gagaatggaa gtttttcgct tttttctttt tagatctatt
    18841 aacgcttggt ggctgctttt tttgggcgaa aatggtcaat aatcttgtcc acttaccact
    18901 cttttttgca ctcttgaatt atggcttatg gattgcgata gcgttagtga ttgtttggcg
    18961 accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta ttttttttgc
    19021 ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
    19081 ggatgaataa atgcgagtca aaggacaaaa ttcagttgaa aggaaatcca ttttgcagtt
    19141 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
    19201 gggagtctta attactagag aagatcattt tcaacgttat tatcgtgtag tttctacaga
    19261 cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
    19321 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
    19381 acaaatcgtg caaaagcggc aattattaga taaaaatcgt atggaacagt ccttaggaag
    19441 aaatctacaa cgattaaaaa aatatgaaaa gaaattagtc gaagaaatcc aggaattgaa
    19501 acgagcagaa caagaacgtc ctgacttaag cttctttttc gtcattgaag gaaagacaat
    19561 caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt tagggttaaa
    19621 accactctac aaaaaagaat tgatcaagat tctgtatcga atgaacaata tgaatgatga
    19681 gtaggaatgg agaagagaga atgaaatatc cgttgatatg gttagaaaaa gatgttccga
    19741 aggaagagac cactagaaaa tttcctcaca ctttaagcct gggtaagttg ggaaaagggc
    19801 aatcttttaa ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt tagaagcaat
    19861 taaaacagct ttttcaccac aaaatgatca aaaagtactt gatccattaa agcaactagg
    19921 aaatcgggaa atagaaattg taaaatccaa agggtatgat ttagattttt taaaacaagt
    19981 gatgccacaa ggtgggttaa attttgatga agaatatgtg acgtatggta atggttattc
    20041 acgatgtgtg acagtttatc actacacaaa aaatcctacg cttttttggt tggccaatct
    20101 catgaacaat cctcatacag tggccacact cgatgttagc acagatgaaa agcaaaaagt
    20161 gattgatcaa atcaatcgtg ccttagatga attaaatgat cgatcagata atgaacgaaa
    20221 agcgactgac cgaaataaaa gcgcggaaga atacctggaa ctagcggaat atgctagaaa
    20281 attgacttct aatggggaaa tttctaagca agtcaaagtg agagtacatg tttacggaaa
    20341 cacgattcaa gaagtagatg aacgagcgca ggaactgatc gaaaatctca aagggatgga
    20401 ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat
    20461 gttcgatgaa caagctgaat ggttcggaaa tatcaaaggg caatcactac catcgttaaa
    20521 cattggcgga ggtttcccgt tccaccacca aacgctaaaa gatccaggag ggacacaata
    20581 tggggaaacc atgacaggcg gcccatttgt ctggaatgcg acgtataaaa atagtgtacg
    20641 gttgtctact aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa
    20701 aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa
    20761 agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag acggatctga
    20821 cggaatgatc aatcctttag agatatttgc cacacggacg tatgatgaag catccagttt
    20881 atatgacgat ggatcagtca aagatttgaa gatcaatgaa gcagcgtcct atcaaacaca
    20941 cttagacaaa gtggtctatc aggtacagtt agtgagtcct cagctaaaag gaacgatgaa
    21001 agcagaattt aagacctact taaatcgttt ttatgaagag tatggaacag taccacgcgg
    21061 ctttacttcc tcaaattcgc gttcaaatac tgatacgcaa gtcacaggga aagatccaga
    21121 agcttatccg acgttcaaag aatttttaga ctttttagga caactggaat taccaggtgc
    21181 ttcccaagag aaaaagaatc gaaaagaaga aatggaatcc atcgtagaat ccttatgtga
    21241 gacgtatggc atgatttttg atggtcatag tacgattcga caccttgacc aacaacagtt
    21301 ggtctttttt gacattgact ctattacagg cttagatcga ggggtacaac aatgtcaaat
    21361 gtacatggct atgacgttga tttggaatca ggcattgatt caaggaagaa gacaacgacg
    21421 tttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg cgatgattga
    21481 tgagtgccat aatggtgcga cctgttctgc agtgaaaagc tgtgggttgc aagtagtcta
    21541 aaaactattg taataactct tataacgaga agtcaaatga gtgggtaacg ccacaaaggg
    21601 cttccactga tactccgaca tgcaacaagt agtttatgca gaccaacgtt gtgtgaagct
    21661 cggtgaagtc ggctgaagga taccgtaagg taaaagaaat agtgaatacc acgaaagttc
    21721 tctagaggta aagggtgtat ccgataagtc gggtgtccaa aaaaacttaa tatggtgagt
    21781 atgtacaaac gtactgacga actagcgaat gtactgtccg aaaggcatag ctcctagaaa
    21841 tgggagagac agtgagcaaa aaatcactca ttgtgctaac ttgtggttga gtaaaagtcg
    21901 ttgttatgaa agaccatgct gcgttacagg cgtaggagaa gttagggggc aaaagctagc
    21961 atctaagtta aggcatgtgg atagatataa gggaacaggg gaagccatca acatggagag
    22021 tgcatactcg atgaagtggt agtaagaaat atgtgaatga caaataactt actttagtga
    22081 tggtgaaagt agagtcacga ctgatgaagg ttctgtaatg gaactggagg aacaggctcg
    22141 attcgtctgt aaaagtcatg aaacccgttc aaccatggga ttctcgtatg actaaaaagg
    22201 tcacgagcca attggagcgt gataaaccat gacaaaagaa acaagtccaa aagataggaa
    22261 gttgcggcat gcagaatatt atggcatgac ggaaatcttc gataagatat actccaaaag
    22321 tttgaaggga gataatttca agaatctaat gcagattatc atttctgata acaacattgt
    22381 cctagcatat cgtaatatca agagaaatca cggtagtgtg acaagaggag aagatggagt
    22441 aacgattaaa gatattgaaa atctcaaccg taatgaattc gtagaaattg ttaggaagcg
    22501 attcgcacat tataaccctc ggaaagtaag gcgtgtagaa attcctaaat ataatggtaa
    22561 attaagaccg ttgggtattc catcaatatg ggatagaatc gcgcagcaat gcatcttaca
    22621 agtattagaa ccaatatgtg aagcaaaatt caataatcac agctatggtt tccgcccaaa
    22681 tagatcagca gaacacgcaa tagcagattg tatggcccga atgaatcaaa gtcacatgga
    22741 atatgtggta gatgtagata tcaaaggttt cttcgacgaa gtcaatcata ctaaactcat
    22801 gagacaaatc tggtctatgg gtatacgaga caaacagctt ctcttaatca ttaggaaaat
    22861 gttaagagca cccgtagtac ttccaaatgg gaagttgata tacccaacaa aaggaacacc
    22921 acaaggcgga atacttagcc cattacttgc gaacatcaat ctcaacgaat ttgactggtg
    22981 gatagcaaat cagtgggagg aaaaagatgc caaagagcta aaacatcatt ttaggaaaaa
    23041 tggagcttgg gataaaggta atatctatca caagctcaga aagagtacaa ctctaaaaga
    23101 aatataccta gtgagatacg cagatgattt taaaattttc tgtagaaatt ataatgaagc
    23161 gaaaaaagtt ttctatgcaa ctaaaatgtg gttacaggag cgtctaaagt taccaatatc
    23221 tgaagaaaag tcaaaaatca caaatctttc aaaagaagaa agtgaatttt taggctttac
    23281 gatgaaatta gtgaccaaag gtaacaaaaa ggttgttaat acgcatatag gtacaaaagc
    23341 acaaagcatg attaaagtga aactaaaaaa acaaatcaaa cgaattcaaa aaagtcctaa
    23401 tagcaaagat acctttatct atattggagt ctataacagt atggttattg ggattcataa
    23461 ttacttcaaa attgcgactc attgtagtgt agcaatgcga aaaattgcaa gagaactcag
    23521 accaagtttt gaaatacgac tacgagaaca aggaatctca aaagaaggac aatacggaaa
    23581 gtacgaagga tacaagcctt acctacggtc aggacaaata cgatatataa gagggtttcc
    23641 aatattacca gtagggtata ttcaacacag acatccgatg tgtaaaaaaa gaaatattaa
    23701 taaatacacc gaagaaggta gggaagccat tcataagaag caacaaaccg tagaagaatg
    23761 gaagatatcg tggttgtgta gcaatccagt gcttagtaag agagcaacga ttgaatttgc
    23821 ggacaatcga atttcaaaat tcatcgctca aaagggaaga tgtgcagtaa caggtgagga
    23881 gcttatctta tctgaaatgc attgtcatca tattatccct tatcatgaaa gcaaaagtga
    23941 tagctatgaa aacctagtga ttgttactga ggaggttcat cgggtaatac acgctacaca
    24001 aagtgaaacg attgaggaac ttctaaaata tctaaaatta aatccaaaac aaaaagaaaa
    24061 actcaatgag ttgagattaa aagttggtaa cgaagaaata tcttaataaa tatttgtagg
    24121 acgggaaaac agaatgtctt ttatagacga gggaacgccg tatgcggtga aagtcgcacg
    24181 tacggtgtgg actgggggaa aagacggaga taatatcaaa ctcttaccta tcagtatcga
    24241 ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga gaaatgcgta
    24301 aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg ccagaaaata
    24361 tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct acgaagatct
    24421 ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt aaatcaatga
    24481 cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg atcaattttg
    24541 gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta aaattgtttg
    24601 atggaggaaa ataatggaaa accaaaatga aagtttaatc aagcagtatg ttaagcgtcg
    24661 tgcaaagcgg cgcttatttt tgtggttgtt tggtacttct gcaggtttga ttactatttt
    24721 gatcactgtg ttcgtgaccc tatttctaat acttgcggcg ggctcgattg ataattcgga
    24781 ttcagattca agcagtggtg gcgaagcttt tacaggagaa tattcggaag gcttaccgat
    24841 ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgccgtagg
    24901 tgcagcagtg aaatataaat tattaccaag tgtgatcctt tcgcaatacg gttacgaatc
    24961 agcgtttggt acatcggcgt ccgcaagaaa tgatctcaac tattttggga ttacttggtt
    25021 tgatggttgt ctgtttccta aaggaactgc acgaggaatt ggtggcattg aaggtggctg
    25081 gtacatgaag tttcccaact caaaagctgc attttcctat tacggcttta tggtagccac
    25141 acaatccaat tttaatgctt gtgtaggaaa taaaagccct ggcgcaagct tattgatctt
    25201 aggacgaggc ggttatgccg ctgcaggaat tacggaagat agtccgtatt atacaggttg
    25261 tatgtcaatt atcacttcta ataaattaac cgaatatgat gaattcgcca tcaaacattg
    25321 gggtgaaggt ggcaataaca acggcacgat tactggtgaa tggacgaatc cattccctgg
    25381 cagcagttta gataaaagtt ccttcagcgg tggtcaatta tttggaacga atccaggcgg
    25441 tgaattccgt ccgaacggct tccatgatgg attagatttt ggttcagttg atcatccagg
    25501 aagtgagatt catgcggttc atggcggaaa agtcgtgtac gttggcaatc ctggtatttc
    25561 aggtttgggt gcgtgtgtga tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga
    25621 atttgcaaat agtacaggaa attctcgagt gaaagtaggc gatcaagtga aagttggtca
    25681 agtaattggg atacgagata cagcacactt gcatttagga tttactcgaa tggattggcg
    25741 tcaagcacaa ggacatgcat tcattgatga tggtacatgg atcgatccgt taccattttt
    25801 gaatagcagt aaaaaataga tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt
    25861 cttaacagct gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagctgaa
    25921 agaggaacaa gcagcaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa
    25981 gctcaaaaaa aattcctctg aggcacaggc gaagatcgac aagttaaatc aaaccatcaa
    26041 tgagaaaaac caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga
    26101 agtaactgaa gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc
    26161 attaagcaga aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga
    26221 caaattaagt cctacagacg atgatcacca acatgatgat tacggaagtt actcctttac
    26281 attaggtgac gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt
    26341 cgtatttgtt gattatacaa tcggcaatcc gcaattcaaa gatgtcaaac cacaaaaaat
    26401 aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcatagactt
    26461 tagttatttc actcgttagg aggaagaaaa tgagtaagaa aaaaggagaa agacaacgtt
    26521 tcaaaaaacg aaaaagttat cttagatggc tgggaatctt tatgattctc ggctttttga
    26581 ttcttgctag tccgatttat acgaatcaga aggtagatat accagatgag gtaagcacga
    26641 acgccttttt aaacacgaaa gagctgacaa tggtttcaaa agaaaaagga gcgaaccagc
    26701 aattagtttt tgaatttgcg atcgatgatg aagatcaaag cttgttaaaa gaactggcta
    26761 atcttaatta tcgcgtagaa gtaaagacag ctaaagggga ttatcaagca atacgagcca
    26821 aagtgattaa ggtgtctgat gattattttg cagtgaagct aacgcatgta ccagaagaat
    26881 atatcgccat gcgtttaacc attattcctg aaaaaattga tccaaaagtg gatatgcaag
    26941 aaccacaaga tctgattttt tatattcacg aagataaggt caaagatcac gtgaaagacg
    27001 aagattatga acaacatgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag
    27061 cagctcaaaa acaaatggaa tctttccaag caaccattga tttgaatgaa gaattagtga
    27121 agaagctaaa agatcaattg ccttatcaag tcgcagatga tcaggaaaat actgaaaaca
    27181 agattaatag ctaccagcaa gaaattgaaa catcgaaaac gcaactgaaa gatcaagaag
    27241 agatcattca aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaag atttagaagg
    27301 acaggtggag tgaaaaaatg aagcgaacaa acttggctga caaagatact ttcgctgagc
    27361 agaaagtatt gattaaagcc tatctcaaag ttgtaaaagc taaagaaata tgtcgagaag
    27421 tgaatttatc taacacaaca atatctgagt tgaagaatgg tcgaaagaat ttagatacct
    27481 taaaattcag atcatttgaa gctctatatg tagctgctaa aaaatatttt acagctgata
    27541 acaaataaaa aacaggagta ttttatgtga aaaaagtata taaaaaatgc ttatgaatca
    27601 gctatatcac cttaaccatt tgttaatgag atgggagaaa acattaacca cgattctaac
    27661 gcaaacatca agcacataaa acatttaaca ctcctccttt ctggctgaag atttcaacca
    27721 aacaggaaga cagcgtatca attatagcat acactttttc ttaaattcat ttttcatatg
    27781 acaaaatatt tccttctaat cgacaaagtt agaaggattt tttattgtct aaatacacaa
    27841 ataaccttaa tttttaaaga gcataaggaa acgtgatatc actttctcac gaaaggtgtt
    27901 aggtaggctg tgcctaccac gtttctaaga aagataaatg atatcaaaat tttggatttg
    27961 tgtgtagatt tgtgggtagg atgaaagcga atgtgggcag taaaataagc gtttgtgtgt
    28021 agcaaaatca aaaaatgtgt agggatatgt gtagctacac atatccctac acataataaa
    28081 tttggaggaa aatatggact tattaattag aaatattgaa gaaaagtata ttaataaaat
    28141 tgacaaacgt tgtgaagaac ttacggtcaa gactggcaaa aaatggagtc gaaatcaata
    28201 cctaaaagtt ttgatcgaaa atgattttga tcacgctctt ctgaattata aaaaagacca
    28261 atttgatcgg ttgttagaaa aatttgtgga tattcaaact tataatacga aaactttaga
    28321 agaatatatt gcaacaaata atcaattaat tggtttgtta attgaataat aaaaatagta
    28381 ttgtgaattt ataaaaggag gaaatcaatt ttggcaaaag caacaagaaa acatgtatat
    28441 ctttacgata tgcatatcga agtacttgat gaaataattt ctactcatga agatatatct
    28501 aattatagtg aagcagtcag acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa
    28561 ataaatccaa ttcaaattaa attgaatgca atgggaaaag aaatttctat tttaaccgaa
    28621 atggtttcag agtttgcgga tcttcatttg aaagatacag gaatactaat tggtacaaat
    28681 tcaactgttt atcaagaagc agaagaacgt gtggaaggaa aaattaaatc caatcaaacc
    28741 aaaaaatatt cttctaagtt aaagaaacca gtgaatgaat ctgttcatac cagagtagat
    28801 ccatttgatc caacgaagat tctaaaaaga taaaagtaga aggagaacgt gatgaataaa
    28861 tcgcctgcag ttattcttac ttgtcaattt accttgccca ataaaaaatc tttttcaaca
    28921 tacattgatt atatgacaag agaaaaagca ctggaagaaa tggaacgacg tacgcccgaa
    28981 gaagaacagg aactgcaacg aatcaagaat gctttagagg atttttatat tcctgaagga
    29041 gaaacttttt cggaaagtaa gacggataaa gaattcagtg aaacgtcgaa tgaagctcaa
    29101 tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt taccaaatat
    29161 ctttcttata tgacccgtca ctatgcgtta gaacaaaaga aagaactgac gcaaagggaa
    29221 caaaaagaat accaacggtt gaaccaagca atgaaaaaat atcaaacaga gccacataaa
    29281 aaagcttcag gaaatcttcc tggagttttt tctatggctt cagacgaagt gaaaggaagt
    29341 gatctgaagg agatcaaaca aatcttccaa agaggacaac aaaaaggctc aatcatttat
    29401 caagatgtta tctctcacga caattcgtat cttgaaaaaa tgggcttgta cgatcccaaa
    29461 acagatacct tagatgaaga aggtttgaaa gcagctggaa gaaaaatgat ggagacatta
    29521 ttcgagaaag aacaattgaa tgaaacaggt tactggatgg caacgattca ccgtaacact
    29581 aagcatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa accgtatact
    29641 gagattgaaa atggtgtgag ttatatcgta ccgaagggga agagaagaca agcaacatta
    29701 gacgcaatga aaaccaatta tgtacatgag ttagaacgtt tctcttatga aaaggatcga
    29761 atgatcggta tggagaaacg aaatttattg acacgaaaaa gtgatttgcg taacacctta
    29821 acaaaagtag taaaagaacc agcacgttac gatcgacacg ccatgaactt attaaatgaa
    29881 gtatataaaa ccctacctga aaaacgttcg gaatggaatt atggagatga aaatcgaacc
    29941 aagctttcac catttacaag gggaaaactt gatgagttaa ccaaatatgt tttaacaaaa
    30001 gaacctggat atcaagaata tatacagatt tcaaaagcat tgaaagaaga agccaaaagc
    30061 ctttatgggg aaagcaaaag agaaacaaag aatgcagaaa aaaacgccct ctttgattta
    30121 aaaaagcgca caggaaatgc gattttaaca gaactgaaga gacaggatag tcaaataaaa
    30181 ccattaatag aaagtgtcaa tcagatgcaa tctatttaca atgaacgatc ctttcgaaca
    30241 ctcaaacagt tgccaacaaa tgaatacaag tatagttatc aagaattcgt aaagcgtagg
    30301 aaacaattcc aacaaagagt tgtcacgcag cgaagcttgc gtcaattaaa tcgaacaatt
    30361 aatgcacatg aagaaaaata ccacgcaagt aaagcatatg aagagcaaca gcgaagaatc
    30421 caaatggaac aagaaagaca ataggaggaa gagaaatgaa acaatattta gatcaatgga
    30481 aagtcataga aggatcacta aaagaagaac gaatcgaaca acttcctgat tgtttagaaa
    30541 aagagcattt gtttcagata agcgaaatgt tgagaaacga acagtttgat ccaaatcaat
    30601 ttttagttgt tgagtaccca gctactggtg tgtattgttg taatcatgtg aagggtgaaa
    30661 aatattttat tattcaagaa tacgaaggta agttagcgcc ttattatact acttgggaaa
    30721 taaatgaaga gggaatcaat aatttcccat gtgaaagcat tgaagagtct atttctctca
    30781 cggaatgtta aataaataga gacgtaaaag agtcctgaac gtgattgtcc aggactcttt
    30841 ttcaatgaat tgttttccgc caattcactg agtggtgcga ttcaatttta ttatatcaaa
    30901 agaaagtgag attccaaagt gaagggagga agaagaaatg actcagacaa cgattggtgt
    30961 tgtggttgaa aaaagcaggg ataatctcgt ttttgtgaca gaaattcaaa caggtagagc
    31021 ttttgtagtc acagacaaag ctgcaaaagc gtatcaaaat ggcaatattt taacgttaaa
    31081 tctgactacg aagatgttcg ttgatgcagt agaaaattat ccatttgttt agacaaacga
    31141 tattttacaa ggaggaaacg gacatggcag aagaaaaaga accactagat aatagtcggt
    31201 tgggaaagtc caaaagaaaa ttagtacggt tgcaaaacga attaaataat caatttcaaa
    31261 agctatttga tcaccaaaaa ttgacaaatg ggcaaccaat gaatgacaaa cgaaatggtc
    31321 gttcatggtt taaccagcaa gaaaaaatag aaaataaaat acaaagtttg agagaagaga
    31381 tcaagcaaca agaaaaacag gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg
    31441 gctataacaa gtacggcgga ctggatatga cgatcgataa tattccaaga atcaaagaag
    31501 aaatcgaacg ctttgaaaaa ggtgaatcga ctttttcagc cgccacgatc cgaaaatatc
    31561 aacggaagtt ggaaacatta gaacagctga aagaaaaatc agaaaaagga aaagaaaata
    31621 tcctccctga agtacaagcg atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc
    31681 caaccattta ttttttgaaa ggctatcgaa aagttgcttt agaactgaat gaaaacgggg
    31741 gatttgagga atcaaaaaaa taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg
    31801 aattacttga agaggctgca ccaactgatt ctctgaagga gaaagaaaaa gatcatttct
    31861 atcgagtaga attcaacgaa aatgatccag aaaaaggatt gaaaagttat ggtggggaga
    31921 tcgtaacatt agaattgatc gatcaactta aaacgttgga taaccaatac gctgcagaag
    31981 caggttatta taaattctat tttgaagaag tgaagaacgg agaagtagta gatcagatcc
    32041 gaatggatct aggggatggc gtcaacagta atcaccccat ctatttagag ttagcaaaat
    32101 tgtgcgaaca agtacctgag aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga
    32161 aagaacaaat gcagtcgaaa gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg
    32221 cagaagagat caaacaagaa gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa
    32281 agcataccca ttcttctgaa gacgtcaaag aacttttgaa ctttatgtct cgttttccag
    32341 aacgatcctt tagaaatcaa ttattgattg aggaacaatt tccaggtgca acggcttgtt
    32401 taggtcgtgc cgcgttgaaa aaagaaggaa tctatatcaa aaaaggggaa caagggaaca
    32461 agatctttgt cagaaaaacg gtcaaaggat tttatgagaa aggacgtggc tttgttcgag
    32521 aaacagaagc tacaccagaa gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca
    32581 aaaaacctta ctacacgatt gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg
    32641 aagactatcc gaaaatcttt ccgaaccgtg tctttgattt tcaattggat aaaaaaggac
    32701 agtcagaact tcaagctggg attcaagcgg tagccaacaa tattggaata caaattcgag
    32761 atatgacaga aagtgaagtc tatcaaagag aattaggaca agcacgtggg gcgtatgttc
    32821 gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac tcgaaatact cctacgcagc
    32881 atttagctac aagtattcat gaattagccc atgcaagaat gcacaaattt tcagaattag
    32941 ataccgcaac taaagaattg caggcagaaa tgacttctta tattgtgtgc aaacattttg
    33001 gaatggacac ttcggagaaa gctattcctt atattgcagc atggacgaag aatggtcaga
    33061 cgttggatga taaggaagcg gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg
    33121 caaatgaatt cattcaaacc atttctaatg aaatcaatca atacagagaa agagaaccag
    33181 agatccaacc agcagaagtg aagcaggagc ataaaaatac tgtagaaacg gcagagaata
    33241 agaatattgt gcccgttggt tcactttgga tcgacaagaa agacgggaaa gtaatggaag
    33301 aagatacggg tcgaacgtta catttgaagg atgcggcgtt tattgaagat ccgcagcata
    33361 atcaagtgat tttaaaagga aaaagttatg gaaaagagat tcaaacggcg ttaccttcag
    33421 aagaatttgc gctaggaaat ccattccgat tttcacttga aacagtgtca gaagtggcca
    33481 aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca aaaagaacgc gtataaaaca
    33541 agtaaaaaag actatctgaa aaacggatag tcttttttct tttgggaggt ttgaaaatga
    33601 ccgaaaaaaa aatgagtctt attgatcgat gtaaacagat cgatatagtg gattttgctc
    33661 gaaacaatgg aatggctgta gtcaataaag gacgagacta tcgcttagaa gaccatgatt
    33721 catttgtttt tgatcgaaga aaacaacgtt tttattggaa cagtcaaaat attagtggcg
    33781 acattattga attagctaaa ctttttttca tagacaaaga gatccaagac tctaagcaac
    33841 aatttaaagc agccactgat tttattctaa agaatgaaga caaaacagaa cgagtggaaa
    33901 atcttcattt tgaaacagaa aagtacaaag atcaccccgt tgattatcaa ccactcacag
    33961 aaaaaggaag gaattatctg aaggaagaac gaaaactacc agagtggctg attgattatg
    34021 cagaaaaaga agggttgatt gccgaactaa agccaaaata tgaacggcaa aatttcttag
    34081 ttcgagacga tcgtttagat catgcggtcg cctttttatg gaaagatcca cagacaaaag
    34141 agacagtagg ggcgagttat caaggaacgt tcattgatta tgaacgtttt ggtgagagag
    34201 gcacatataa gcatatagat aaaaattcga cagcgaatca tggctttaac ctaaagatcg
    34261 gtgaccctaa gcagttaaaa ttttttgaga gtagtattga tttacttagc tatgctgcac
    34321 tgaatcgtga tcagttaaac gatacgtggt tagtctctat ggaagggtta aaacaccatg
    34381 tgatcagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa gcctttcctc
    34441 aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac gaaaaagaac
    34501 aattaatggg cgcagtcgat ccatttacga atcagaaagt acgttgtgaa cgaggaatcg
    34561 ctaatgattg gcaagtgcca aaagaataca aaatcattta tgaagaagtc gcaaaagaag
    34621 aaaaggtcac accagaagct attatggcca tacacaagac agaaaacaat ctgcaattaa
    34681 cgaaccaact ggtttccgct cacaaagtaa atgcgttttt tggccagcag ctttctgtta
    34741 acgattcaat agaagccatt aatttgaaag atatttgtag agaagtcgca aaagaattaa
    34801 aagtttgtga acgcgtggat ggtacgtacg attttgatcg tttttatcaa gaaaaagggg
    34861 atattaatgc gcagattctt ttctcttata aagcagaaca atactacaaa ggctataaaa
    34921 atcatgaaca tgaatttata cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg
    34981 agatccagca acaagaaatc agaaagcaaa aacgtgccat gttgtttcaa caaggcagac
    35041 aacaagaaag ggagtgaaat cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca
    35101 caagcgaaag cttatgccga tagcttttct aaagctaccc gaaaagatgg gtattttgag
    35161 atccaagatc gattgtttac aggagaaact gtgatcacgt atggttttgg tcatttagtc
    35221 gagttagatt cgcccgatat gtacgatgaa aaatggaaac aatgggcatt agaacatttg
    35281 cctattttcc ccaatcaata ccactatcat gttcctaaag ataagaaaaa gcagtttagt
    35341 gtcgtcaaac gacagctgca gtcagctgat acgattgtga tcgcgactga tagcgatcgt
    35401 gaaggggagc tgattgcctg gtcaatcatt caacaagcag gcgccaatca aggaaaaacc
    35461 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccaacag
    35521 ttacgagacg cagaagagac ctatccaaaa tttgaagaag tacaagcacg tcagatcgcc
    35581 gattggttaa ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc
    35641 ataccaggaa gtttttcttt aggtagagtc caaaccccaa cactctatat gatttatcaa
    35701 ttgcaggaaa agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta
    35761 atcgctcaaa atggcgcatt tgatgcgaag cttgatccaa atgaaaccca agcgacacaa
    35821 gaagcatttg aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca gccaggtacg
    35881 atccttcaag tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt ttctttatcg
    35941 agccttcagt ctaaaatgaa tcaactcatg aaagctagtg ccaaagatac tttggaagcc
    36001 atgcaagggt tgtatgaagg aaaatatcta agctatccgc gaacggatac cccttatatt
    36061 acggaagggg aatatgcgta tctgttggat cacttagacg aatacaagca ttttttaaaa
    36121 gccgaagcga ttccgactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa
    36181 gtccaggaac attacgcgat cattcctaca aaaacggtta tgacagcagc ggcttttgaa
    36241 caactaagtc cactgcagca ggctatttat gaacaagtgt taaaaacaac ggtggccatg
    36301 tttgcggaaa agtataccta tgaagaaaca acgatcttga cgcaagttca gcagcttcag
    36361 ttaaaggcaa tcgaaaaggt gccgctagat ctaggttgga agaagttatt tggaaaagag
    36421 agcgacggaa aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg
    36481 gtgacggtgg atctacaagt tcttgaaaaa gaaaccaagc caccacagcc ttataccgaa
    36541 ggtacgttga tcacggcgat gaaaacagct gggaaaaccg ttgatagtga agaagcgcaa
    36601 tccatattga aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa
    36661 accttgaaac aaaaggaata tatcaaggta gagaaaaata agctcgtggt gacgaataaa
    36721 gggattttgc tttgccaagc cgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc
    36781 gccaaatggg aaagctactt gttaaagatc ggtgaacgaa aaggaacaca aaccactttt
    36841 ctagccaata tccaaaaatt tgtctctcac ttattggaag tcgtaccagg gcaaatccaa
    36901 tccactgatt ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc
    36961 gcgcgtcatt taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag
    37021 gtggcagcat gtacgtctga agcctgtgat ttcaaactat ggacaacgat tgccaaaaag
    37081 aaacttacag cgacacagct gaaagagatc attcaaaata gccgaacaag ccaaccagtg
    37141 aaagggttaa aaggtcagaa gggttcgttt gaagcaacga ttgttttaaa agaagacttc
    37201 actacaggct ttgaattttc tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc
    37261 actacaaaat aatggagggt taatcttatg cgtaatacaa cacacaaaca acaagaaatc
    37321 gaaaatcaaa aagccaaaat tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga
    37381 ctgcagcaat tgaagaacca gcagcggatt caaaaagaaa tcgaattaga tcgataaatt
    37441 cctttcttgt taatataaaa ctaacatgtt atagttgtgt tagttttata ttaactttag
    37501 gggaagagta atgaaagaca aaatccaatg tatttttgat aaacataatg gcaacctatc
    37561 cttgaaggat gctagaagtg aaggattatc gcctatgacg ttagatcgat tagccgaaaa
    37621 ggataaaata gaaaaggtag caccaggttt ctatacctta aaggatgcgt tcgtggatga
    37681 attgtatctc gctcagagta tctattccag agggattttt tctcatgaga ctgcgctcga
    37741 tctttttcaa gtaggaacat atatccctaa aaagatcaat atgatgtttc ctaaaggata
    37801 caatgtatca aaggagaaat tagatcagta tgccattcaa ccgcattaca ctaataaaga
    37861 aaattttgac ctgggagtca cagaaacaga aagcttttac ggcaataaaa ttttagtcta
    37921 tgatttagag cgaacgttat gtgacatgtg gaatccaaga tacaaagcaa gcgtagaggt
    37981 aaaacaagaa gcattaaaag aatatatgac aagtaccagt agaaatacaa ataagctaag
    38041 aagatacatg gatattttaa aatcaccaaa agaaatgact ttgtatatgt tgccacttta
    38101 ttgagaggag taaagatgaa tgacaccgac acaatttaaa gagaaaacaa gaaatttcgc
    38161 agcagagaaa catttaaacc cacaaattgt ccaaagatat tatatgatgg aaaaattatt
    38221 agaatgtatt gttgaatcgc cttatcatga tgattttgta ttaaaagggg gatttctgat
    38281 tggatcaaaa tacgggttag aaaaccgttc aaccgttgat attgatacga cttatcgaaa
    38341 ttcgaaacta acaaaagaaa agttagaaac tgttttttct gaattaacag ctaatcctac
    38401 aaaagatgga attcgttttg aattaaaggg attaacagag acgagggaag ccgattatta
    38461 tccaggtttt caagcaaagt ttattgctta tcttgaaaat gcacgtgtac cttttaaatt
    38521 agatattact tcaggtgatt ctattattcc agcagcattg atgtatgaac acaaattaat
    38581 gtttgaagat aaaacgattc atattccagc ttatcctacg gaacaaattc tagctgaaaa
    38641 attacatgcc acgtttagtt ttggaaaaga taattcgcgc atgaaagatt tttatgacct
    38701 gtatatgatt ccgaaattag aacgtatcga tgagaaaaat ttgtataaga gtgttcaagg
    38761 aacattcaag gaaagaaata atcaatcggt gtttaagcat ttgtatgatc aggaaatgcc
    38821 tcaattggtg aaagatgagg gcttgagaga aaagtggaaa agataccaac aagaaaacta
    38881 ttttgccaaa gacatttctt tcgaatctac actaacttcc gttgattccc tgatgcaatc
    38941 aattgtagag caagagaagg cagaacgaaa acaaaatttt aagagatcac aacaaatgaa
    39001 tcaagaaatg gagagataaa gatgatcaat aatgtaactt tagtagggcg attaactaaa
    39061 gatccagatt tacgttatac cgcaagtggc acagcagtgg cgactttttc cctagccgtt
    39121 aatcgtaatt tcacgaatca aaacggcaat cgagaagctg attttatcaa ttgcgtgatt
    39181 tggcgcaaac cagcagaaac gatggcgaca ttagctaaaa aaggcagctt gatcggcgtt
    39241 gtcggacgaa ttcaaacacg gacttatgac aaccagcaag gacaacgtgt gtatgtgaca
    39301 gaagttgtgg ccgataactt ccaactatta gaaagtaaag cagcgacaga aagccgtatg
    39361 cacgctgatc aatcgtccag cagtccaagc cctaccacct ttgaacaacg ggatacagcg
    39421 acgcctaaca acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat
    39481 attagcgatg atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt
    39541 ttatgttata atttagacaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg
    39601 gcgggctagt ggctttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc
    39661 tttcttggca aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat
    39721 gattgcaaaa acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat
    39781 taggccgggg tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg
    39841 atttttgcta taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata
    39901 aaatccgagt aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg
    39961 aatcgattaa ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg
    40021 atggaaaata caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg
    40081 ctagcatgta tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaataaaag
    40141 aagagttgta gtcctaaatg aggattgcag ctctttttgt tttacataga aacaggaaaa
    40201 gaccttttta ggaagacaca agtgccttct caattgaaaa acctttctct cactccaacc
    40261 gccaccgccg ttcgagaaga aaggaagtga acgctgcacg ttcactaaga tatggtgtct
    40321 tgcaaagcaa gacaaaatag acaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
    40381 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
    40441 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgatttattc
    40501 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
    40561 ggcagaatca aacatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
    40621 cccaaactat catgcaccac atactgttga atgcgattgt ggagaaagag cgaaatgtat
    40681 caaaagctat cgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg
    40741 cgattacatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca acacaaatta
    40801 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
    40861 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
    40921 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
    40981 aacatggaaa cttatgaaat cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
    41041 aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac
    41101 aaaaaatgga ggatatgacg ttcctatacc atgaaacacg tgtacccaaa aaacattaca
    41161 aggaaaaatt gtcagtcgca gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca
    41221 ttgcgacgta ctatcgaaca ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc
    41281 aagccttact atgcttggaa gtaggtgtca aaccaagcac tatcaaacct tccgaatatc
    41341 aagccttaga actgacttat gcaaaattcg cacagattaa gaaagctaaa gcaatggcta
    41401 gcgactggct agatactttt gaaagtatcg aaaaaaatgg tgcattttat acgattgaat
    41461 cggaggagaa agataatgaa taaggtggtt tatcagctaa caacagaaga tgtttcgcaa
    41521 tttttaacaa gcaaagaaca cttgaatgaa gtaatcagac ggattcaaaa agactttagt
    41581 tgtatggaag aaatgaagta ctttattcaa agagtggaag aacagttgta tatggaaagc
    41641 gaaacaagtc aaacgaaggt cacagtcagc cataatcatt tagacgtgta taaacgtcgt
    41701 cctgaaacgg taaaaaaagg aacagagctt ctacgactag aaacagaaaa atacatcatg
    41761 aatgtgtacc tagaaaggag tgagctggtc gaaaactttg tcagctacac gatttgtagg
    41821 aaacactatt actcagaatc agaatattat tcaattttcg agagcatgga attaatggaa
    41881 gaagaaatgg aagagctaaa cagcaaaaat gcgacggtag acgattatta ccttgtattt
    41941 actaggtttt tgacgtgtgg gacggaatct atcgtgttaa tgggaatgga ttatgaactc
    42001 gctgtacccg aagcattttt gagggaatat gtagaagaag tagataaccc aggcgacgac
    42061 tggtacacga ctttaactga ttttttagac acctacactt acgacgatac agaagaaatc
    42121 caaaatgaac ttgaccgtaa acgaatgcca tatcaacgat ttgaaaggaa aaaaggtaaa
    42181 taaccattta taccgaaaag tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa
    42241 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    42301 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    42361 ttcctctatt gttcgtactg tttgttggcg agatacttat gcgtcatgct gcagctgtac
    42421 atttaaactt cgtagtattc ttaggaatac tggtattttg tttgatcttt tggcgatttg
    42481 tattagccct tgtgagtcta ctattcaaga tgattgcagc catatttttc atagggttat
    42541 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    42601 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    42661 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    42721 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    42781 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat
    42841 aactatgaaa tctttaaagt atccagaaga gattattttt agatcaattt ttttcttacc
    42901 tgattctaaa gattttgttt tcttaaacat attatgattt cctttcgact attttcatat
    42961 aaatgttaac atagtttatg gattcatgga atgttaatct aatttaatag aaaaggataa
    43021 ttaagtatat acgttttttc ttatgtatgt tatactatgt atatacagaa agaggtgtct
    43081 actatggaaa tgttacctat aaaaaaatgg ggaaatagta atggccttag attgccaaag
    43141 tatattatgg aatatcttgg aattcataca gaagacaaag tgaagataat acaagaagaa
    43201 gtaaacggtc agaagcggct aattatcgaa gcggctaata tagaaaatga attaacaatt
    43261 gaacagttat ttgaaaatta cactgatgaa agaaatcatg tgacgattca aaatttaggt
    43321 gaagcagtag gtaatgaaaa atggtgagaa aacctagaca aggcgatatt ctattattga
    43381 atacagcacc tcgatctgga cacgaacaga caggtaaaag accttatatt gttttaagtc
    43441 atgatattat tgcggattat agtaacgttg ttacagttgc tccaatttct agtacaacta
    43501 gaaactatcc gctatatgta aatataaact cagaatatga aatgaagaca acaggaaaag
    43561 tattattaga tcaactaacg acaattgatt atgaagccag agagtgtatt tttttggaaa
    43621 aagcgcatga tagtttagtt gaggagttat tgatgaaagt tagaacagtt tttcaaaaag
    43681 taagcaaata aaatagaaac agcagcatga tcaactattc gtgattgacc atgctgtctt
    43741 tttttattct atatacggtt tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg
    43801 caacaccttt ttttagatcc tcaacgatgt ttttataaac aagacgacgt tggggatctt
    43861 ttgcattggc actgtatagc ttaggacgtc ctttataaac accgttcgct tttgcaatct
    43921 taatgccttg tgcctgacgt cgtttagatt cggtacgttc ttgttcagcg atcatggcca
    43981 aaatttgaat gatcaaatct ttaatgaatt tatccaggag aggattgcca attgcttcgg
    44041 ccatgatcgg tagacttgtg atgatcagct gcacattttt cttcttcaag taattgactg
    44101 aagcgatgat ctcgtcgtaa tttcttccaa gtcgatcaat agcttccaca ataaaaatat
    44161 cttgatctcg gacgaaatcc agtgcttctt gaaaaacaag tcgatgagta atcgttgcgc
    44221 cagattgttt ctcaatgaaa attctctctg caccgaattt tttcatttct tcaatctgcc
    44281 gttgttcatt ttgatcgatg gatgagacac gtatgtatgc aactctcaaa atttttacct
    44341 actttctgca actatttaaa aaacacccta atcatacacg agaaaaccag atttttcaat
    44401 gtcgcggtcg tgtctagtta gggtatacct tatatagaca aaaactaatg atggaggaaa
    44461 tagtatgtgt cacttttctt tttaaatggc aatatttttt gtaaataaat gtcaatcaag
    44521 aaaatttaaa cga
//
LOCUS       DAFCHQ010000013        40885 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000013 DAFCHQ010000000
VERSION     DAFCHQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.013"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1398)
                     /locus_tag="LNC71_000828"
     CDS             complement(<1..1398)
                     /locus_tag="LNC71_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBK6465920.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIF"
     gene            complement(1409..2077)
                     /locus_tag="LNC71_000829"
     CDS             complement(1409..2077)
                     /locus_tag="LNC71_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBK6465921.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2207..2791)
                     /locus_tag="LNC71_000830"
     CDS             complement(2207..2791)
                     /locus_tag="LNC71_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6465922.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3122..3355)
                     /locus_tag="LNC71_000831"
     CDS             complement(3122..3355)
                     /locus_tag="LNC71_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBK6465923.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3370..4320)
                     /locus_tag="LNC71_000832"
     CDS             complement(3370..4320)
                     /locus_tag="LNC71_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK6465924.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4317..5159)
                     /locus_tag="LNC71_000833"
     CDS             complement(4317..5159)
                     /locus_tag="LNC71_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK6465925.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5161..5880)
                     /locus_tag="LNC71_000834"
     CDS             complement(5161..5880)
                     /locus_tag="LNC71_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK6465926.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6572..6718
                     /locus_tag="LNC71_000835"
     CDS             6572..6718
                     /locus_tag="LNC71_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6465927.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNT"
     gene            6790..8016
                     /locus_tag="LNC71_000836"
     CDS             6790..8016
                     /locus_tag="LNC71_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6465928.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8104..8496)
                     /gene="rpsI"
                     /locus_tag="LNC71_000837"
     CDS             complement(8104..8496)
                     /gene="rpsI"
                     /locus_tag="LNC71_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK6465929.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8512..8955)
                     /gene="rplM"
                     /locus_tag="LNC71_000838"
     CDS             complement(8512..8955)
                     /gene="rplM"
                     /locus_tag="LNC71_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBK6465930.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9474..9941)
                     /locus_tag="LNC71_000839"
     CDS             complement(9474..9941)
                     /locus_tag="LNC71_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBK6465931.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10061..11599)
                     /locus_tag="LNC71_000840"
     CDS             complement(10061..11599)
                     /locus_tag="LNC71_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBK6465932.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11679..12365
                     /locus_tag="LNC71_000841"
     CDS             11679..12365
                     /locus_tag="LNC71_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBK6465933.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12566..13093)
                     /gene="msrA"
                     /locus_tag="LNC71_000842"
     CDS             complement(12566..13093)
                     /gene="msrA"
                     /locus_tag="LNC71_000842"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6465934.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13177..13854)
                     /gene="pgmB"
                     /locus_tag="LNC71_000843"
     CDS             complement(13177..13854)
                     /gene="pgmB"
                     /locus_tag="LNC71_000843"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBK6465935.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13851..14981)
                     /locus_tag="LNC71_000844"
     CDS             complement(13851..14981)
                     /locus_tag="LNC71_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6465936.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14981..17164)
                     /locus_tag="LNC71_000845"
     CDS             complement(14981..17164)
                     /locus_tag="LNC71_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBK6465937.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17161..18186)
                     /locus_tag="LNC71_000846"
     CDS             complement(17161..18186)
                     /locus_tag="LNC71_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6465938.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18231..19058)
                     /locus_tag="LNC71_000847"
     CDS             complement(18231..19058)
                     /locus_tag="LNC71_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6465939.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19055..20122)
                     /locus_tag="LNC71_000848"
     CDS             complement(19055..20122)
                     /locus_tag="LNC71_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBK6465940.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20148..20984)
                     /locus_tag="LNC71_000849"
     CDS             complement(20148..20984)
                     /locus_tag="LNC71_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6465941.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20981..21850)
                     /locus_tag="LNC71_000850"
     CDS             complement(20981..21850)
                     /locus_tag="LNC71_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6465942.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21920..23203)
                     /locus_tag="LNC71_000851"
     CDS             complement(21920..23203)
                     /locus_tag="LNC71_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK6465943.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23217..24902)
                     /locus_tag="LNC71_000852"
     CDS             complement(23217..24902)
                     /locus_tag="LNC71_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBK6465944.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25177..26202
                     /locus_tag="LNC71_000853"
     CDS             25177..26202
                     /locus_tag="LNC71_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6465945.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26797..30471)
                     /gene="rpoC"
                     /locus_tag="LNC71_000854"
     CDS             complement(26797..30471)
                     /gene="rpoC"
                     /locus_tag="LNC71_000854"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBK6465946.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(30529..34146)
                     /gene="rpoB"
                     /locus_tag="LNC71_000855"
     CDS             complement(30529..34146)
                     /gene="rpoB"
                     /locus_tag="LNC71_000855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK6465947.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34874..35842
                     /locus_tag="LNC71_000856"
     CDS             34874..35842
                     /locus_tag="LNC71_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6465948.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35860..36765
                     /locus_tag="LNC71_000857"
     CDS             35860..36765
                     /locus_tag="LNC71_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465949.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            36762..37568
                     /locus_tag="LNC71_000858"
     CDS             36762..37568
                     /locus_tag="LNC71_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6465950.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37704..38693
                     /locus_tag="LNC71_000859"
     CDS             37704..38693
                     /locus_tag="LNC71_000859"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBK6465951.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(39010..39534)
                     /locus_tag="LNC71_000860"
     CDS             complement(39010..39534)
                     /locus_tag="LNC71_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBK6465952.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39821..>40885
                     /gene="scm"
                     /locus_tag="LNC71_000861"
     CDS             39821..>40885
                     /gene="scm"
                     /locus_tag="LNC71_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK6465953.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        12026 a         8442 c         7252 g        13165 t
ORIGIN      
        1 aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt tttttattcc
       61 accatttttt aaataaataa ttctattgca tgttttataa agaaattccg gatcatgagt
      121 agctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa tcctttccat
      181 attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag tttttgacaa
      241 tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa acggtaattg
      301 atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat ctttactaac
      361 tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt ggtaatccat
      421 ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta tctttccttt
      481 ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca caccgttttt
      541 ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt ttagaatatt
      601 ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga tatagggaac
      661 ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa
      721 caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa
      781 ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa
      841 atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc
      901 atccaatata attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg
      961 ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc
     1021 agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga
     1081 aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac
     1141 gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa
     1201 taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt
     1261 taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac tgcctttttt
     1321 tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac
     1381 attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa
     1441 cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt
     1501 tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag
     1561 ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag
     1621 acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag
     1681 tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt
     1741 tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac catgagatca
     1801 ttacgaccgg caacattcga aatacaatat ataaggatgt cgcaaaaaat atagtaaaaa
     1861 actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga
     1921 tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga
     1981 aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat
     2041 ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga
     2101 tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta
     2161 tcaaaaagca taactgagaa caattctcaa tgcaagcttt ttttaattaa ctcagtaatt
     2221 caatccaccc aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt
     2281 ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt
     2341 ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat
     2401 ctagacaaca gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca atatcttgat
     2461 atttggttcc agctgattgg aataaaatca aatcgaatat acgtttattc tcaaaaatat
     2521 aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct atatctatat
     2581 tttctaagtt taagaggtat ttggtaaact cttcattata catattaata agcgcattaa
     2641 tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag
     2701 cacccgtagt tacgcctgcg gtcaggcaca gttgccttat cttagtttgc tgaaatcctt
     2761 ctttttcaaa atggcttagt ccactttcta ttaacttttg gtgagttata atataatctc
     2821 tggtttctat gggtacttcc tcctaataag atgaattaac tttattatct tacacaggac
     2881 ttgaagtttc aatcgttaac gtttattact gaactcattc cttagagcta aactaatgag
     2941 aaagagcaga ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat
     3001 cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg
     3061 ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa aataaagtct
     3121 tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa
     3181 atcctatatc taaatagtcc tcatatctaa gtttagccag catctcactt acagatgctg
     3241 gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata
     3301 attcctgaat tgctagtaaa taaattagta tagtagtcgt tttaatttgt accataccta
     3361 ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc
     3421 atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata
     3481 gataggaata ttggtatctt ttgatactgt tttcattggt ctatcatcca cactgctttc
     3541 tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc
     3601 tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc
     3661 tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat
     3721 tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc
     3781 tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat
     3841 atttttagcg taaataatac cgttttctaa atttaaccaa gcatgaggat cttccttccc
     3901 tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc
     3961 tttgttttcc tctttgttcg catttttaac taattttgta aaccaagcat ttcctccagt
     4021 ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc
     4081 aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat
     4141 tttatctttt gctatatttt cggtaatatc tgctaaaatc gaattagtaa ctactacttt
     4201 taatttttcg ttcgaagcca ctttatcttt gtctttacta tttattgaac atgccccgaa
     4261 tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat
     4321 aacgatctct ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca
     4381 aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca
     4441 ataaacaatc caagcaatga tgaacttgct ccgactgttg cagaaataaa tatcatagtt
     4501 ttcaagctat ttgcatataa atatgctgta gctgctggtg taattaacat tgcaacaatt
     4561 aatatagttc ccacactttg catagctgtt actgctacta aagttaatag aaccatcaat
     4621 agataatgat aaaaactgac attcatccct attgcttttg ccattaatgg atcaaaagaa
     4681 gtgattaata attctttaaa aaatagaact actataacca acactaaaac cgaaacgcca
     4741 attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa aatatgaaat
     4801 aaatccgttg agctatttgc aactcctata aggataactc ctagtgctaa aaaagaactg
     4861 aaagtaatac caattgctgt atctcctttt attacactat tgctttttat aaaagtaata
     4921 atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata
     4981 taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt
     5041 cctcttaaaa taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta
     5101 attagagcat tctgtaaaaa gtgaaagtca tacagaccat taataaaatt ttgaagcata
     5161 ttattccccc tctccaataa atatagtatc tccataggct ttttttaaat tttcttcatt
     5221 aaatacagat tctacacttc cgtgagcaat tagatttcga ttcaaaagaa taatattatc
     5281 aaaatacttc tttaccttgc ttaaatcatg atgcacaatt agcactgttt ttccctgatg
     5341 tttcaatttt tttaacgtat ccataattat tcgttcacta actaagtcta tccctacaaa
     5401 tggttcatct aaaaatatga aatccgcatt ttgagcaaga catcttgcta aaagtacacg
     5461 ttgaaattgt cctccagata attctcctat ttgatgatta gaatattttc ccatatcgac
     5521 cttttccaaa gcctttgaaa ctctttgcca ttctgcattt ttaattcttt gaaaaacttt
     5581 cattccggca tatgttccca tagatacaca ttcttttact tttattggaa aagtaaaatc
     5641 aatatcagct ttttgctcta catatgcaat cttcaccagt tttttattga tatcttcatt
     5701 attaatggag acctctccct gatgagggat aatatttaac attgctttaa gcaatgtgga
     5761 ttttccagca ccatttggac caataatacc tgttaaagca ccttcttgta tttttaaaga
     5821 aatctcgttc aatgctaaaa aatcgttata acaaacatta agctttttca cttctaacat
     5881 aactttctcc ttctaaatga taatgattat cacgatcata tggtatacaa aaattatatt
     5941 ttagtcaacc ctaaatttta tagtttagat ttttttctgt tgctgaagat tagaatgaca
     6001 aaactatttg aaactacgcg tatagtaagt agctttcaaa atatataaaa aaggagcgtt
     6061 ctcaaatgag aacatgctcc taaaactact ttattggcgt atggaaaaat tatacttgaa
     6121 gtggatagcg ggttgaataa gttgataagc atcaaaacat ttataaaaat tattcaagac
     6181 tatgtctgtt ccatatacaa atatgaaaat cagtaaactc catttaactt ttttcgtagt
     6241 gtgtcttttt atacatgttt atcgttatta atagtgaata ggaaaaaaac tatttttgat
     6301 cattgacaat taaatacaat ttttgaataa cgatccctgc tattcgagct agaaactcaa
     6361 gtacgccatg acctgattag cgacaaatac ttagagattt ctttttgttc tgggaaaaca
     6421 aaatgccatg acaatagcag caaataatga tacttccgta aggctcgccg taaactgggg
     6481 agaggtgaaa ttcctatgtg cttacccaaa gcaattcaaa aatttcataa ctactatttt
     6541 aggagatgat tttatagaat ccagagtcta cctgaataaa aaacaaacct gtgcatttct
     6601 aggaatcgat aataatactc tagataagtg gattcgtata tataatttcc cagtaataag
     6661 agttggtggt gtagttcgct ttgacgtaga agatattaaa gactttatga atacttgaaa
     6721 ttttcttctc gccgcatata ctaaaattag tgaaatatac tgccttactt tttttaagga
     6781 gtgaggattt tgcctacaaa gctttcaaat gggaaatata aaactaatct gagatatcca
     6841 aaaaggttta aagaaataac aggaattgca tcagaaaagt atcaaaaaac atttccaaat
     6901 aggcaattgg cgattaaagc cgaaaatgat atgaagaaga aaatagaaaa agttttgaga
     6961 gaggagaatg caaactcatt agaattaaaa ggaaaaataa cgtttaaaaa attttacgaa
     7021 tctaaatggc ttcctagata tgaattaggt caaacaatta gatcaaacag gcctcctagt
     7081 gatataacca ttagtaatac aaaagatata tttcggttgc atattttacc tatgtttggt
     7141 gaatacgcaa tgaattatct aaatatcaat actgagataa tatcagatga attaaccaaa
     7201 aaatcgaaag aatatgcaaa tataaaaatc ataaaaggat atgttcgttc catgttcgat
     7261 attgcagaaa tactaaatta tatcgaattt aatcgaacta ctaaaattat tcaaagtatc
     7321 actgcaccta aaaaaaatgc tttagaagaa aaaagaatac aagaaggtaa gcaagcacta
     7381 tcatcaaaag aacttactaa ttggatagaa gcagtaaatg atgattttaa taatcattta
     7441 cttacttttc atgattacac actttttatg attaccctgt atcttggtga tagaaaaagt
     7501 gagacttacg ctttgcaatg gaaatatatt gattttgaaa aacaaacggt ccgtcttaaa
     7561 catactttag ataaatatca aagaaaaaag tttacaaaag gtcgaaaaga taccgtcatt
     7621 caagtaccag aagttgtaat gacattgcta agtgaatgga aatcggtaca agctgatcaa
     7681 ttgttgaaat taaaaattaa gcaaacgtta gatcagtatc tctttactta tactaaacca
     7741 tctggagaag taaattgtcc tgttcatgcc gattacctaa actatcgaat caattcaatt
     7801 aaaagaaggc atcctgactt agttcattta tctccacaca aactccgtca tacttatgca
     7861 actatagcta gacaaggtgg agcagatatg aatcaaattt caaatgcttt aacacactca
     7921 gatatttcta caacaaagat atatgtgaac acaccagata tagttgataa ggctgttttt
     7981 gaagcttttc agagaggatt gaataagtgt gattgatatg tgttcttaaa tgtgttctta
     8041 aaaacaaaag aagcactttc caaataattg aaaagtgcca gaaacgttga tatataaagg
     8101 atattaacgt tttgagaatt gtgaagcttt acgtgctttc ttaagacctg gttttttacg
     8161 ttcgaccata cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta aacggaagtc
     8221 aggatcaact tctagcaatg cacgagcgat accatgacgg atagctcctg attgtccagc
     8281 gtatcctccg ccgtttacgt taacaaatac gtcatatgcg ccttttgttt cagtaacagc
     8341 gaaaggttga ttgattactt cacgcaagtc agcatgtgga atgtattctt caacatcttt
     8401 tttatttaca gtaattttgc cagttcctgg tactagacgt acgcgagcaa ctgcgttttt
     8461 acgacggcct gtgccgatat attgaacttg tgccaatgaa attccctcct attagattaa
     8521 gtttgtgatg tctaaaactt ctggttgttg tgctgcatgt ggatgttctg ctccaccgta
     8581 tacgttcaat tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca taccttttac
     8641 agaagtttcg atcaaacgac gagaattttt agcacgcaat tcgccagcag tgattgattt
     8701 caatcctcct gggtagttgc tgtgacggta ataaattttg tcagtcgctt ttttacctgt
     8761 taatttcact ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt
     8821 gaaagttggt ttatttttac cacgtagtac agatgcaaca actgttgaaa gacgtcccaa
     8881 tggaacatca gttgcgtcta ctacatacca tttacgttct acttcgcctg gtttggccat
     8941 atatgttgta cgcacgttat tttcctccaa tttaagttct gatgtttgac atacacttcg
     9001 agtttccggg gctctttgtg gggcaaacaa taccatttaa catattaccc gctaatctag
     9061 tcaatgtcaa cgtttttctg cttgattgaa aaatttatat gaaactttac ttcttttctt
     9121 tcttttatat aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa
     9181 agtcttgtca caacccctta ttttgaataa acggtgttct cctgccaact cctcggtcac
     9241 aagtcacaat atccataaaa cataagaagc tgttttttaa aattgtttct tgtgactcgg
     9301 agctgaacgg caggcttaca acctcttggt aaacggtgtt caatcagctg accctcggta
     9361 ttagctcaaa aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca
     9421 ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg
     9481 tcgatctctg gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct
     9541 gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg
     9601 attccttttt ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt
     9661 tctggaatag tacgatccca gcggccaatt tcatcaggga tttttgtgtt atctgcaaat
     9721 tcacgcaatg tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct
     9781 aactgagcgt tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct
     9841 ggtcctctca tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca
     9901 actaattgat ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat
     9961 tttcttacac ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct
    10021 gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta
    10081 attcttctcg cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa
    10141 caaataatgg atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct
    10201 ctttcaattc tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttctcc
    10261 agcctaaaat cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt
    10321 aacccagtac atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag
    10381 cacggatctc tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga
    10441 gacagatccc ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac
    10501 cgataaaact gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg
    10561 gatgaaaaat cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat
    10621 catatggatt ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc
    10681 ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct
    10741 gatccagaac ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa
    10801 aacgaagtgc tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta
    10861 accctagatt agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca
    10921 aacctagttc tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga
    10981 cttgcggcaa ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga
    11041 attcatggat atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc
    11101 caaacaatct ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata
    11161 attcaggttg gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg
    11221 gcgtcccagt ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt
    11281 cgtccgctgc tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat
    11341 ccaacaatat caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg
    11401 aaggcaactg tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct
    11461 gatcttgttc tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg
    11521 ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta
    11581 atccgattga aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt
    11641 aagcttgcgt atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg
    11701 gttgctgtat cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta
    11761 ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga
    11821 ttccgattct ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc
    11881 ccttctacta tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt
    11941 tttcttccgc aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt
    12001 tgatggattt attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt
    12061 tatggatttt ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta
    12121 tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt
    12181 tgatgctttc ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg
    12241 ctagtctgtt tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg
    12301 aaaagctgcc tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt
    12361 attgaaaaaa ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac
    12421 caaatccttg tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc
    12481 aatcagctga ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg
    12541 ttccataagt agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac
    12601 ctgcacgtcc agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat
    12661 cctgatggta atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg
    12721 gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt
    12781 cttcttcatc attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc
    12841 ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa
    12901 taacttctgg atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta
    12961 cttgttcata agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga
    13021 tccctggctg ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg
    13081 ctttttttgt catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg
    13141 ataacgtttt gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga
    13201 taaatgccaa aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc
    13261 agaaggaaga tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc
    13321 ttgacaacca gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg
    13381 ctgtgctcct ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc
    13441 aataaggaca tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt
    13501 gcttgctgat cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat
    13561 tgcaccaggc aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt
    13621 tttttgctca gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa
    13681 tgacagcaat cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt
    13741 ctcagtgaat ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg
    13801 agctgtgtct acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta
    13861 cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga
    13921 ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa
    13981 acggccgaaa tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata
    14041 gctatcacag gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt
    14101 agactttgtc catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt
    14161 ttatcgaact ccagtaaatc caatacgtag tcgatgccag agactatttt ccctgaagca
    14221 aatgctaaca gtcctgcacc cattccacca ccagcactac tgcccgatgc ctcgattact
    14281 tgaggggcaa atctcatata aaaatcatgc atggcttggt cagccactgc taattcattt
    14341 tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga
    14401 ttagtaacat cagtaatgac agtgattttg gctttaatca gctcaatagg cacgttcgtt
    14461 ttatcaactg tagcaatttt acctagattt tctcccagtg cttctacttt ttgattatct
    14521 tgatcgtaaa aagcataacc tagaccttct gccatgccga tgccgccatc gttggtacta
    14581 cttccgccaa cgccgatgat gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt
    14641 tctcctactc ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg gacatctttc
    14701 aatccgacga tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt
    14761 tctttgtttt tgccaaatgc attagtaacg actgctgttt gccgcttcaa ccttaaactg
    14821 tctgctaatg atgaaaccgt tccttctcct ccatcgccta taggtagcag atcatattca
    14881 gcctgaacaa aaacttcttg aaaacctgat tgaatggctt gtgctgcctc taaggctgtc
    14941 agactttctt tgaaagaatc tggcgcaatc agaatacgca ttattttccc ctccgttcca
    15001 agatcagttc tttttccagc tggtattggt ctccattgac aaaaataggt aatgtttctt
    15061 tcgtctcttt ttttattact atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt
    15121 tccaaataaa aggaaaagaa attttttccc atgctgaagg aagtcttgga gaaatagaaa
    15181 gagttccatt cgttactgta atacctgaaa aaccaaatat aactgctagc catagtgctc
    15241 ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg atctggtcct aaatcaatca
    15301 ggcacgcttc ttggaataaa tgatacgccc attccatttc ttggatacgg caagcctcta
    15361 tggcgtgaat cgctttactc aacgatgagt cgtggattgt tttgttttca tagtaatgca
    15421 aattcttaga aacgattggt tgagaaaaat actcaggtaa taagtataac agcatgacaa
    15481 gatctgcttg cttgctgact tggagttcat ttacttcgct acgggcataa tcaagtaaaa
    15541 tagactggct gccaggacgt tctttgtaag tggtcaaatc aataactggc ttactgagat
    15601 acgtgtcatc ttgcggaatc agctgttctt tgttaggcac tggcaaatat aacttatcta
    15661 aaaaattttg acattctttc aaaaaaagcg gatcgccagc ttgatattta acagctaagg
    15721 ccacattata atgcgccaga tagttcgtat atgcattatt gtcgatatat tctgtgtatt
    15781 catctggacc aatgactctg tgtatctcta aatgatcatc gacttttaca gctctgctca
    15841 gccaaaaagc tgctgtttca tgaatgagcg tttttccctt tgtttctaaa aacgattgat
    15901 cctctgttgc ttggacatac tcttgaacgg cataagcaag gtctgcactg atatgatgtt
    15961 ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt gatattcaat gcagcaaatt
    16021 tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt
    16081 gataatctct tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat
    16141 tctttgctac ttcaggaaac tgatataaaa agaaaggcag gataaaaatt tccgtatccc
    16201 agaaaacatg ccctttataa ccttcgccag ttagtccctt cgcccctaca ctaacacttg
    16261 gatcattgcg gggagtcata atctgtaaat ggtaagcagc gaagtccatc gctaactgat
    16321 cgaaagaaga atcgccttgg atcgttatcc gatgctgatg ccaaaaatct gtccaataag
    16381 acatcgactg ctctaaaatt tctgtataac taacgctttt ctgggcttgg gcaatacttt
    16441 tcttaaacgg gtctgtttgc tgaagagagg tagagagcac gatgatcttc tccagcgtaa
    16501 aggacgtatt ttttctgatc ttttctgaaa aggaagcggt gatccggcga ttttttgctt
    16561 ggaatacacc tgctttattg aatttagctg caatcccaat atcaatttta ctttctgttg
    16621 tctggtagga tgcactgatc aaatcgtcac caaatactcg cagttctttt tcaaccaact
    16681 gctgtgtacc aaaattggtt tgctgtccat cgatacccgt tttcacatct acttggcagt
    16741 cttcggtcaa cggcttgatg accactttca gggctgcctg atggagatta ttttgatcag
    16801 caacacgaca agtaatgact tccacttttt ttccttgagc agtttcaatg agcgcatagc
    16861 gtttaagctc tcccatttct aaatctagtg cccgctcgta tgcaagaact ttcatcgtct
    16921 caagagaaaa atgttcaccg tcgattttta tttcccatct gatgacatct gggagattca
    16981 ctaactcggc tgattctgct cctaaaggtc ggttatatac accagctgca aacattcccc
    17041 ttacttgatt ccgatagtct tcctcctggc ttcctctgat accaaaaaat ccatttccaa
    17101 tggtcatctg actcgcatac ttattgatat actctaaatc aaactgtttt tcttctactt
    17161 tcataaggta ataacttctc ctgtttctgc cgaacgatat aaattttcca ccagattctg
    17221 gatgacatat ccttgccagc catctgcaat catctgagtc tggtcaccga gcgcccgatt
    17281 cacaaaatga gccatactta gttcatgtgg gtcattaact ggcgtggtct tttgtaagga
    17341 aaccagcttt ccatctacat cttcgaatag ttcgcctgga tacagattag cacctgcctg
    17401 atcgccacaa aaagccacat tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt
    17461 cagttggatg atcttgccgt tttctaattc cagtgtgcca aaaattgcat cttctactgt
    17521 gaatttgctt ggatcccatt tcccgaattt ccctgaattt tttttcgtac caattttttg
    17581 gaagctgtac gcccgaacgc tttttacttt aggaaatcca gtaatataca tcgctgtatc
    17641 aagcatgtga atgccataat cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa
    17701 cgttccccag cctggaacac cagaccgacg aagcgcattc gcttctacgt aataaatatc
    17761 cccgagaaca tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa
    17821 tccataagcc aaaatctgcc cgtttttatc ggctgtctct gccatctttc ttgcttgatc
    17881 agctgtcata gctggtggtt tctcgcacat cactgaagca ttagcttcga gggcagccaa
    17941 tacatgttcg aagtgaaagc gattgggtgt acagacactc acgatatcgg gagaaatctc
    18001 attcagcatt tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc
    18061 ttctgctcgc tggctgactg gatcaaccac agcggccaat tctaagacat ctgatctttt
    18121 ttgataataa gcagcatgta ctttttctgc tacttgtcca gcgcctatga ttgctacttt
    18181 cttcatttat ttctccctcc tttgcttatt tacttattgt tattctttct ctattctttc
    18241 tctaaatcat ttaaatgacg cgctgtttcc agacagtttt tcaagtattg caatgattcc
    18301 tgataaattt tcacttcatt ttctcctcgc aaacgacatt catataccat ataaccttca
    18361 tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt
    18421 tggtagcgat gattatctgc taaatggata tgacccacat actctttttg cgtagtcaat
    18481 gcttttgaaa tatcatcttc ttcaatattc atatgataaa aatcagcgat gatctgcaca
    18541 tgtttcaatt gattagagtc aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg
    18601 tcttggtatc ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc ggctacttta
    18661 tctaaataac gaagtgattc gctgactgct ttatagtctc cttctttgct tcggggtgaa
    18721 accatcggcg gtaatcgata agtgaacatt ccccaagctg ctgggataac gattcctttg
    18781 cctccaacct cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc attaagacgc
    18841 cgttcttcga taaagtcacc gatccaccca tcgtagccgt tacaagctgt cactactggg
    18901 atccctgttt ctttgattgc ctgtttgact gtgtcgatat tttctattaa caacttaccg
    18961 tcgatttcgt attcttcaaa tccacagtct ttgataaatc taaatttttc taaaatgtct
    19021 cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg ttctcctcct cggacttgaa
    19081 cgcccatttt gatactttct tctgggtgct ggtctacata acgcatgtaa gcttctgcgc
    19141 tttctgcaaa tggcacaact ggatcaatga tttcttcaca atccaagtaa ccgttcatca
    19201 ataattccca acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa tctgggtttg
    19261 gttcactaga tgctcgagca aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa
    19321 gattaaaccc agctggaaaa ggtttagcaa aggcaacata agaaatgatc ccgccatagc
    19381 cgatccctct taaagaagct tgtaatgcgg cagcactgcc actcgtttca ataactgaat
    19441 ctactccacg tttatctgtc aattccttta gacgaaaacc gacatctgct ttcataggat
    19501 ctagcgtgta atcggcaccg tatttttctg ctaattctct acgctgcgct atgggatcga
    19561 tcccaacgat ggtagtagca cctgcttttt tagctaattg aatcgcaatt tgcccgatag
    19621 cgcctaaacc gaagattgct acagcatcac ctgctcgaac atgtgcttcc cggataccac
    19681 ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt ccattgatcc aaccgatcaa
    19741 gtttacgtag tttataatta tcaacaccgt ttgtaatcag cgtttcacga atgggtccat
    19801 aagtgcagac taactctcct ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg
    19861 taccgacaat catatttcct aattgaaatt cgccaaatac cacaccgcgt tggcttcctt
    19921 cttctcttgg caagaatagg cgccattctt ctgaaaattc ttcatcgata aatggactta
    19981 atcctctaaa gtcgacaatt tctgttcctg gttttggagt agcgaaaatt actcggatac
    20041 gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt gaatccggct attcttggtt
    20101 ctaatgcaac taattttttc atcttttctt tcctctttct tttcttttta tccttttacg
    20161 ccgccttcga ctaaatcacc tttaataaat cgttcagata acgcatacat aatgatgact
    20221 ggtaaagcgg tcaccaatga agcggccatc attcgtcccc aaatgtagtc tggtgtactg
    20281 aataatgtgt tcaaaccgat tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa
    20341 gcaaataaat aatcattcca cgaaaccata aagcaataaa cgaagacaga aatgattcca
    20401 gaaatagcca atggtataat gatccgaata atgatctgga tccggctaag accatcgatc
    20461 attgctgctt cttcaatatc agtcggaatc gtgtcaaaat aactttttag catgaatatc
    20521 gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt atcatacaaa
    20581 cctagaccgg aaatgatttt aaataatgga acaacgatca aaataccgga aaacatataa
    20641 acggtgtaaa agctagcatt gattttcgag cgagctttga agcgtagttt tgacaaagcg
    20701 tatgccccgc tgatcccaat cactactgca gttacggagg ctatcaagga gatcactaaa
    20761 ctatttttga aatacatgat gaatggaaaa atctcagcat tgaaaatatc ctgataatgt
    20821 tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc
    20881 ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa
    20941 atcgtcgcgt aaaataagat tttcctgctc tttttatttt tcatttcaag tccaccttct
    21001 ttcgtgagag caagacgacc gctgcaataa tgatgaacaa tagaatcgat atagctgctg
    21061 cttttccaag atcattgaac gcaaaggctg tctgatacaa ataaacgcct aagatattga
    21121 ctttattcgt cagtagatag acatctgtaa acatatagaa catccagata gatcgtaaaa
    21181 caatcacagt tgctagtact ggtttgattg ccggcagtgt cacgatcttg aacttctgcc
    21241 aagcatttgc accgtccatt tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa
    21301 tagccaaaaa tgaaataaac gcatacggat agtatctcca gatagcaaac aaaacgacca
    21361 taaagaagct agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc
    21421 tatctactaa cagatcattg acgattccat aactattatt gaacatatat ttccaagcaa
    21481 aaatcaaaga aatagatggc gtaacataac ttaataaaat cagtgaacgc accgttttcc
    21541 taaatctgaa cttgcggtta agcaaaatag ctacggctaa tcctaaagct gtactgcctg
    21601 ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga acgataaaat tcactatcac
    21661 ttaaaatttc aagataattg cttaatccga taaaaacggc gtctgagtta ggattcagcg
    21721 gcatatcgaa aaaactgatc tgaatattgg aaagcattgg aatcagcact aaaacagcta
    21781 ataaaagcat gcttggtgct aaaagtccca tggctaattt taagtcagaa tgtttccttg
    21841 gacgattcat tgaaacctcc ccctgataat ctgccaatag ctatctactt ttttcgaaca
    21901 ccattggcag aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga
    21961 catcttcgtt tccgacagtt acttggttca ctgcttttcc gatcacacct gagcttgtga
    22021 tatctcccat tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt acttcatcaa
    22081 aagcactagc aatctcagat ggcagctcgc caaaagactt gatgacttca ttttctttgt
    22141 aaccatccag ttcgactact tgtttattca ctggttgagc accaccagga gacatcaaga
    22201 cccacttttc catgtttttc ggttgagata agaaagctgt gaatgcttca gcagcctctt
    22261 tttgtttctc gtctagacca gaagaaatcg tcaatccaga tactgtgcca tagacagctt
    22321 gatctttttg tgttgggata gcaaaaccga tattttctga gttccccgct tcataaacag
    22381 aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt tccattcatg aaagcatctt
    22441 tgatttctgt cacatcatta gagcctggca tcgtgtattg ggataagttt tgataaaacg
    22501 acaatgcttc tctcatcttt tccgtatcga tcgttacctc gccttcatca tccaaaacat
    22561 ttgctccatt tgacaaagca aattgagaga acgcttgctc actcattgtt ccttcagcag
    22621 taggcatagc aattccatat tgtttattct ctttatctgt gaaatgttga gcaattttta
    22681 aaatatcatc ccaattttct ggttctgaaa aaccagcttc tgataatttt tctttgtcat
    22741 accaaatacc ttgaacccag ccgctaattg gtgcagcaat atagcttttt ccatcttctg
    22801 aacgaacaag gtttttggct ccgtcataat aattatcttc tccagcttcc tcgataacgt
    22861 tttggactgc cgtctgatca atcagttcat ctttatccat cactttagca aaatcctgac
    22921 tgacttccat tacttctggt aatttaccgg aacgagctaa cgtaacgatt tttgtattga
    22981 acgctgattc ttctaccggc acttgcttga ttttgatgtc tggattttct ttttcaaaat
    23041 cagcaaccag ttcattgatc acacttaatc gttcctgttc gacagaagag tgcatgaatt
    23101 gaatcgttgt ctgttgtgat tcgttagagc tttgactttc accacaagca gtaagaagaa
    23161 atccagtagc tagcagacag gtagtaacaa taattttttt catttttttc cacctcttat
    23221 tttattattt tcagccacat atattcatac ggctggaata tcaactttcg ttcttgactg
    23281 caatggataa tttgtccaga aaacaggtct aggtaatctc cttttggtaa aagataacct
    23341 gctgattgat tggaaaaatt atggataact aagactttct cttccttcgc ttcgcgaata
    23401 aaactgataa aagaatctgc tgagtccagt aagtaaatca aactattcgg atggaaggct
    23461 ttttcttttt ttcgaataga tagcatctgg gtcaattcac catatactgc tgttcttaat
    23521 ggatctgtct gcaactcttt ctcaatatct gctaacaaaa atttttgccg attgattttt
    23581 cgattctctc ctgttttttc tacgcctttg taatcatttc gacttcccag cacactttga
    23641 atataaattg ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt
    23701 ttgcttctcc attccgtcgt atctgttgga gaacttaacg catctagata cgtagcgttt
    23761 atctcatatg gactggtcgt tccatctggg tttttcttgt agtttactcg ggtaccctct
    23821 ttcttcagct gttccactaa ttcaaggatc gttttttccg gcagaatacc tcgtaccgga
    23881 ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg
    23941 aaatcgatgg tttttaacca ttctttcaac ttacttgtat tacccgaacg aatcgcttga
    24001 agggtcaaag gtggtagcga aaactgatag accatctgtg cttcattctc gccattccca
    24061 aaataactga tattatcttt gtgtggcaca tttgtctcag tgatcaatac cgttcctgga
    24121 gctgcttcat cagcgattgc acggaataat tggatgattt tatgcgtttc tggcaggtgt
    24181 atacaagaag ttcctagttc tttccataaa aatcccacag cgtccaagcg aataaatgaa
    24241 gcgccttttt ctaagtaaaa caaaagaata tcgatcattt tcaataaaac ttctggattt
    24301 ttaaaattca agtctacttg atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg
    24361 tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg gtctagtcac gctgcttgta
    24421 tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa gtttaaatat
    24481 tcctgaaacc aagtactttc ggcagacata tgattgcaga caaagtcgaa cattaaccgt
    24541 gtttcgcttc tcatttcttc tatatcttcc catgtgccca aacggtcgtt gattgcctga
    24601 taatttacaa cggagaaacc atcatcagat gtatatggga aaaatggcaa gaaatgtaca
    24661 actctaaatg tatctgccaa atacttttga taaaatttct taaatgtacg caaagttggt
    24721 tgctgctctt caaaaaattg atccccataa gtaatcaaga aaatatcttc ttcgtcccaa
    24781 tgcagtttgc ctgcctggcc tttcttctgc tgataaacac tgactttttg ttcaatcata
    24841 gaggcggttt tctcagacaa atctgagtcg cgataaatcg cttgtaaaag ttttgtatac
    24901 atcggattcc cctttctttt ttgggagcgt tcccaacgat ttgatcatat tatggaaaac
    24961 gcttactgtc aatagcattt ttttaaataa ttttgatata cacaaaaata caaataagaa
    25021 agcttatgta tcaataatta atagcttctt aaaaaaatat ggtctctcat gatcatgcaa
    25081 gctattccta aattttcaaa atcatagaca aaaaagaatg tctattgtac tatggaatta
    25141 ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga aacctactat ttatgacatt
    25201 gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag tattaaataa ccagccaaat
    25261 atctctgata aagcaaaagc aagtgtactt caagcaatca aagaattgaa ttatgaaccg
    25321 agcaaaattg caagaggact gtcaggaaat ggttttgacg cgattctagc attatctcat
    25381 cgtacttctc atacgaccta tggcaatcct tttttttcag atgttattca atccatttct
    25441 gccgtttctg aacaaaataa cttcgacttg attctacaaa cggcaaaaac accagaagaa
    25501 gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat tattatttta
    25561 agctctacga tggatgaaac acttcttttt gaattggatc aatacaatat tcctattgtg
    25621 gtaatcggaa aagtcggaaa cgactacacg aacatctact cagtggacac ggataatttc
    25681 caggatagtt acgatttggt ggaaacactg atcaagaatg gacaccgtca tatcggctgt
    25741 ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc
    25801 ttatttgatc accacctaga tatccgtaat gaatggatta tagacggcgg ctttcgctta
    25861 gaagatagca tggcagcaat gaatgattta atgaagctac gagaaagacc aactgcgatt
    25921 ttttctaccg atgcattaaa atcattgagt atttacaaag tggcagaaca atctggaaag
    25981 cagatacctg atgatctttc gatcgcagga ttcagtgatc ccttattgtc ctccctattt
    26041 tcgccacagt tgactcggat agacattccg acaaatcagt taggaatcaa agccacagaa
    26101 cttttattcc aactaatcca ccaccaatac cctgaagaaa aaagaatcat tctttcaacc
    26161 gagattactc tcacagattc gatccaacaa caagaaatgt agaacagaat tataaaagaa
    26221 cagaaacact gaggataagt cagttactct gttctttttt caatcactca tccctcgtta
    26281 ccccctcccg gtattttagc gaatttttct tattttccat ctgttttaga tagctgtgga
    26341 taactataat gtttgtgtta aactattttt tccacaggga agaaaaaact atttttaacc
    26401 acgttgtgta taagttttta tagttcgttt ttttaaacac atttaccctt atttcaatcc
    26461 atgttatttg gggatactta tctctaaaac ataaatagaa taacagacat gtctactggt
    26521 tttgttttat tcgaggaatt attcaataat agctagcaaa atattaaaaa taaccgctct
    26581 atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga
    26641 cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga
    26701 tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg
    26761 tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc
    26821 gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac
    26881 accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat
    26941 cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc
    27001 agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt
    27061 ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc
    27121 tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg
    27181 taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat
    27241 tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc
    27301 acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc
    27361 gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat
    27421 acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc
    27481 tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat
    27541 cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg
    27601 agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc
    27661 aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga
    27721 gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt
    27781 gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc
    27841 ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc
    27901 tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc
    27961 gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac
    28021 atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc
    28081 tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca agacggatag
    28141 tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat
    28201 caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc
    28261 agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct
    28321 gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag
    28381 accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc
    28441 ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga
    28501 atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa
    28561 tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa
    28621 ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg
    28681 tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat
    28741 gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc
    28801 agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac
    28861 atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc
    28921 ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgggaagg
    28981 agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat
    29041 acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc
    29101 gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac
    29161 taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa
    29221 tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc
    29281 gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat
    29341 cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt
    29401 caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa
    29461 gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg
    29521 gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc
    29581 ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa
    29641 acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt
    29701 tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat
    29761 aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc
    29821 caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa
    29881 ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc
    29941 catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt
    30001 caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa
    30061 ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg
    30121 aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg
    30181 ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt
    30241 ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat
    30301 ccgctcacag aacaaccctt cgcgttcagg ttttaaggta cgatagttaa tcgtctcagg
    30361 tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg
    30421 catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca
    30481 aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt
    30541 atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc
    30601 agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac
    30661 agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat
    30721 atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga
    30781 ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac
    30841 acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc
    30901 gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt
    30961 acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt
    31021 gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc
    31081 aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc
    31141 ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac
    31201 gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt
    31261 catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag
    31321 gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc
    31381 ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat
    31441 gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac
    31501 gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc
    31561 gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt
    31621 gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat
    31681 ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc
    31741 aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta
    31801 tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc
    31861 ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga
    31921 tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg
    31981 ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa
    32041 tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc
    32101 atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc
    32161 aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat
    32221 caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac
    32281 tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc
    32341 actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct
    32401 gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc
    32461 agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt
    32521 atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt
    32581 ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc
    32641 gcggtcacga gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc
    32701 gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt
    32761 gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc
    32821 ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg
    32881 gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc
    32941 gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc
    33001 tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg
    33061 ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac
    33121 accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag
    33181 tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc
    33241 tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt
    33301 tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg
    33361 agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc
    33421 gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt
    33481 ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga
    33541 accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg
    33601 tacataagag atgtcttttg catcagtttc catttctagc caagctccac ggttagggat
    33661 aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc
    33721 tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc
    33781 ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt
    33841 ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc
    33901 acgagcttct gctactgtat actttggttc tttcaattca taatctacaa attccaaaga
    33961 tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc
    34021 atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac
    34081 ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc
    34141 agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca
    34201 ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa
    34261 aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt
    34321 ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt
    34381 tttgcaactt catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt
    34441 ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat
    34501 tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa
    34561 cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta
    34621 gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta
    34681 tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt
    34741 ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt
    34801 ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa
    34861 ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc
    34921 tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat
    34981 tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga
    35041 aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc
    35101 ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga
    35161 tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat
    35221 cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga
    35281 aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg
    35341 cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga
    35401 agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa
    35461 agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc
    35521 taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt
    35581 tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt
    35641 caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggta aacaaacagg
    35701 gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc
    35761 gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga
    35821 cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt
    35881 ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc
    35941 actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca
    36001 gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc
    36061 tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt
    36121 cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg
    36181 atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca
    36241 ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt
    36301 gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc
    36361 gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt
    36421 tatatgattt ctaatggatg cattgctctt tctggctcct tgcttgcgca aaacaacggc
    36481 tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc
    36541 gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt
    36601 gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat
    36661 tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa
    36721 aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat
    36781 tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag
    36841 gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg
    36901 gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt
    36961 tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc
    37021 gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg
    37081 ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc
    37141 gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct
    37201 cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat
    37261 tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt
    37321 ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta
    37381 tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc
    37441 aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat
    37501 tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat
    37561 cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct
    37621 ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc
    37681 tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca
    37741 aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc
    37801 aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct
    37861 atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa
    37921 aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga
    37981 cttctgaatc tacgatgaaa gatgtcaaac tggcagcagc tcaaggagat tctgtttcga
    38041 agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg
    38101 ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg
    38161 aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa
    38221 ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct
    38281 gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca
    38341 ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa
    38401 aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca
    38461 aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata
    38521 aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg
    38581 tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga
    38641 agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag
    38701 atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag
    38761 actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa
    38821 tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat
    38881 cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc
    38941 atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta
    39001 gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg
    39061 catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca
    39121 gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc
    39181 aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg
    39241 ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa
    39301 ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg
    39361 gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc
    39421 tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc
    39481 aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt
    39541 tcctcctttt tatttatcct acttactttt tcctaattct aggataccta cttatatgtt
    39601 actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga
    39661 aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt
    39721 aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga
    39781 gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt
    39841 actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga
    39901 aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt
    39961 gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca
    40021 tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct
    40081 tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa
    40141 ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt
    40201 ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac
    40261 tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt
    40321 tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac
    40381 tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac
    40441 cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat
    40501 cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa
    40561 cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga
    40621 tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt
    40681 tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac
    40741 gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat
    40801 atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag atacatctac
    40861 cacaagtgat agctcagatt catca
//
LOCUS       DAFCHQ010000014        39172 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000014 DAFCHQ010000000
VERSION     DAFCHQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.014"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(22..966)
                     /locus_tag="LNC71_000862"
     CDS             complement(22..966)
                     /locus_tag="LNC71_000862"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBK6465954.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1262..2206)
                     /locus_tag="LNC71_000863"
     CDS             complement(1262..2206)
                     /locus_tag="LNC71_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6465955.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2426..3640
                     /locus_tag="LNC71_000864"
     CDS             2426..3640
                     /locus_tag="LNC71_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6465956.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3662..5677
                     /locus_tag="LNC71_000865"
     CDS             3662..5677
                     /locus_tag="LNC71_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBK6465957.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5760..7133)
                     /locus_tag="LNC71_000866"
     CDS             complement(5760..7133)
                     /locus_tag="LNC71_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK6465958.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7159..7443)
                     /locus_tag="LNC71_000867"
     CDS             complement(7159..7443)
                     /locus_tag="LNC71_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6465959.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7445..9460)
                     /locus_tag="LNC71_000868"
     CDS             complement(7445..9460)
                     /locus_tag="LNC71_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6465960.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9639..9944)
                     /locus_tag="LNC71_000869"
     CDS             complement(9639..9944)
                     /locus_tag="LNC71_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBK6465961.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10033..11063)
                     /locus_tag="LNC71_000870"
                     /pseudo
     CDS             complement(10033..11063)
                     /locus_tag="LNC71_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11270..12145
                     /locus_tag="LNC71_000871"
     CDS             11270..12145
                     /locus_tag="LNC71_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6465962.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12297..13139)
                     /locus_tag="LNC71_000872"
     CDS             complement(12297..13139)
                     /locus_tag="LNC71_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6465963.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13231..15108)
                     /locus_tag="LNC71_000873"
     CDS             complement(13231..15108)
                     /locus_tag="LNC71_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6465964.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15105..16526)
                     /locus_tag="LNC71_000874"
     CDS             complement(15105..16526)
                     /locus_tag="LNC71_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6465965.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16529..19918)
                     /locus_tag="LNC71_000875"
     CDS             complement(16529..19918)
                     /locus_tag="LNC71_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBK6465966.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20175..21566
                     /locus_tag="LNC71_000876"
     CDS             20175..21566
                     /locus_tag="LNC71_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6465967.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21695..22969)
                     /locus_tag="LNC71_000877"
     CDS             complement(21695..22969)
                     /locus_tag="LNC71_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6465968.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(23148..23795)
                     /locus_tag="LNC71_000878"
     CDS             complement(23148..23795)
                     /locus_tag="LNC71_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBK6465969.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(23803..24489)
                     /locus_tag="LNC71_000879"
     CDS             complement(23803..24489)
                     /locus_tag="LNC71_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6465970.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(24495..25568)
                     /locus_tag="LNC71_000880"
     CDS             complement(24495..25568)
                     /locus_tag="LNC71_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6465971.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            25790..26464
                     /locus_tag="LNC71_000881"
     CDS             25790..26464
                     /locus_tag="LNC71_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6465972.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            26469..27809
                     /locus_tag="LNC71_000882"
     CDS             26469..27809
                     /locus_tag="LNC71_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6465973.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(27958..28944)
                     /locus_tag="LNC71_000883"
     CDS             complement(27958..28944)
                     /locus_tag="LNC71_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBK6465974.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            29668..30699
                     /locus_tag="LNC71_000884"
     CDS             29668..30699
                     /locus_tag="LNC71_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6465975.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            30808..32340
                     /locus_tag="LNC71_000885"
     CDS             30808..32340
                     /locus_tag="LNC71_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBK6465976.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            32479..33291
                     /locus_tag="LNC71_000886"
     CDS             32479..33291
                     /locus_tag="LNC71_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBK6465977.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            33281..33766
                     /locus_tag="LNC71_000887"
     CDS             33281..33766
                     /locus_tag="LNC71_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6465978.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            33789..34334
                     /locus_tag="LNC71_000888"
     CDS             33789..34334
                     /locus_tag="LNC71_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBK6465979.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(34407..36050)
                     /locus_tag="LNC71_000889"
     CDS             complement(34407..36050)
                     /locus_tag="LNC71_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBK6465980.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(36179..38407)
                     /locus_tag="LNC71_000890"
     CDS             complement(36179..38407)
                     /locus_tag="LNC71_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6465981.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(38440..38919)
                     /locus_tag="LNC71_000891"
     CDS             complement(38440..38919)
                     /locus_tag="LNC71_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465982.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11397 a         7434 c         7181 g        13160 t
ORIGIN      
        1 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
       61 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
      121 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
      181 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
      241 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
      301 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
      361 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
      421 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
      481 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
      541 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
      601 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
      661 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
      721 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
      781 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
      841 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
      901 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
      961 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
     1021 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
     1081 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
     1141 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
     1201 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
     1261 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
     1321 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
     1381 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
     1441 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
     1501 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
     1561 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
     1621 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
     1681 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
     1741 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
     1801 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
     1861 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
     1921 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
     1981 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
     2041 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
     2101 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
     2161 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa atgtctaatt
     2221 cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac
     2281 gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg
     2341 acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat
     2401 aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct
     2461 gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat
     2521 gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc
     2581 ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa
     2641 acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc
     2701 acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat
     2761 caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat
     2821 gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg
     2881 acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat
     2941 tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg
     3001 cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt
     3061 tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac
     3121 gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc
     3181 aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct
     3241 tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct
     3301 gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta
     3361 cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat
     3421 cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc
     3481 ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt
     3541 gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag
     3601 agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa
     3661 aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg
     3721 ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg
     3781 gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc
     3841 ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca
     3901 tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg
     3961 acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg
     4021 ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga
     4081 tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga
     4141 aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga
     4201 actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt
     4261 agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa
     4321 agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca
     4381 acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc
     4441 ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt
     4501 gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc
     4561 attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc
     4621 attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt
     4681 gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat
     4741 cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg
     4801 gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga
     4861 aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg
     4921 cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca
     4981 agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg
     5041 cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt
     5101 cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt
     5161 attgaatgac actatcacac agattactac tccatatttc aaattctatg aacaagatgc
     5221 tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg
     5281 gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa
     5341 tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc
     5401 aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta
     5461 tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc
     5521 tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga
     5581 tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc
     5641 acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata
     5701 tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct
     5761 taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat
     5821 acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct
     5881 agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg
     5941 attttaagat ctcctaatac tggaaggata aataatggca gccaactaat caataagcta
     6001 ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca
     6061 gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag
     6121 cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat
     6181 acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt
     6241 acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc
     6301 ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt
     6361 acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca
     6421 agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag
     6481 ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt
     6541 ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg
     6601 tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt
     6661 ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact
     6721 aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat
     6781 ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa
     6841 gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact
     6901 actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg
     6961 attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg
     7021 atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg
     7081 aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt
     7141 tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt cttttacttc
     7201 ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt
     7261 atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac
     7321 atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt
     7381 gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt
     7441 catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta
     7501 ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa
     7561 gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat
     7621 tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc
     7681 catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat
     7741 actgctccac gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata
     7801 atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt
     7861 ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta
     7921 aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt
     7981 ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc
     8041 ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca
     8101 tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag
     8161 aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct
     8221 ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc
     8281 taatgccact tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg
     8341 cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc
     8401 tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag
     8461 catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac
     8521 gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa
     8581 attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa
     8641 aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa
     8701 atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa
     8761 tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt
     8821 ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg
     8881 cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat
     8941 aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca
     9001 tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc
     9061 taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta
     9121 tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa
     9181 aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt
     9241 gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg
     9301 ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt
     9361 gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt
     9421 ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact
     9481 tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga
     9541 ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga
     9601 ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt
     9661 tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt
     9721 tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa
     9781 gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct
     9841 tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt aggggccatt
     9901 gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt tcctcctagt
     9961 agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac aaacccgatt
    10021 cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc cttcttccaa
    10081 aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc cattaactaa
    10141 tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa ttactttttc
    10201 ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt cgggcaaatc
    10261 gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt cttgtcgatc
    10321 ttttgtttgt ttcacgaaca tccctttttt ccgtaaacga cgaaacttgg acgttctttg
    10381 atgcggaaat aactggattt tatcgaaatt ttcatatttc catagaaaaa atccagatcg
    10441 aagtaatcat tcattcgtcc tttccctaaa agttgacgta catcatagct gatatcatct
    10501 ggttcgccga aataagaaag aacttgatct actgtatgac aagcatgtcc atataaatag
    10561 ctgttcgcag tagaaaattc atgcgtattt tctggtactt ccggtctgaa ataatcataa
    10621 tgcatttcta cttctaaaat atcccctaat tttccgcttt cgattacttt ttggactgtc
    10681 aagaagtcag aatcgaaccg tctgttttgg taacattgga taaatagacc tttttctttt
    10741 gctaatgcaa ataactcttc tgcttccttt gacgtttctg caaaaggttt ctctactagt
    10801 acattcttac catgttcgag tgccattttt ccatattcat aatgaaattg actaggtgta
    10861 gtgatgacaa ctaattggat ctcaggatca tcccaaagat cagacagctg atcagtatat
    10921 tcgatgtctt cgaatgtttt ccacactggt tcagatgggg aaaaaatttt tttgatattg
    10981 attttgttcg taagttttct tgaaaaaggc aaatgatagc gatttgtact ctttccattg
    11041 ccaagataag cgattgtaag catgagtggt gcctcctttt attgatgttt tcagtatatc
    11101 ctagaaaagc gcagaaagta tttataatag ctattctggt aaaaaagttc agaaaaacac
    11161 ttgttatcat tcggtttggt gaaattaatg ttcaaagtaa ggataaaaat caaaaagata
    11221 gtagcggaac caagtgttta tcagtaagat agaatttgga ggtcagatca tgcttattga
    11281 agaaaaacta acaaaacaag agttgtttac cacgacagag aaacggattg tcgattacat
    11341 ccgtaggaat atcgaagctg cagtctacat gacgattgaa gaattagcaa aagctacgta
    11401 tacttcccat tcagctatca ttcgtttatg caaaaaaatg ggattcagcg gatttaaaga
    11461 attccgtttc gagctggggc gggaagttca tcaattgatt gctcagttca atcaaacgat
    11521 tgatcctaat tttccttttt caagtgaaga tagcactcag caaattgcaa agaaaatggc
    11581 ggatttatcg atccagtcaa taaaaaaagc acaattgcag attgaacatg aagatttaga
    11641 tcaaatcggt aaagtattga acaaggcaca gcgaatcttt ctttttgcaa aaggtgattc
    11701 tcaaatcacg gcaaggaaat tccagaataa aatggtcaag ttgaataagt ttttgatcat
    11761 ggctgaagaa tacagtgatt cttcttggaa tgcagccaat ttgactagcg aggattgcgc
    11821 catttttatt agctacagcg gtcgcatcca tcattatgaa cgaataatga cctatttgaa
    11881 gcatgtagag gcccctacct tgctgattac cggaaatcag cattcagaaa tggccaaaca
    11941 ggctgatatg tgtcttgtta tatcgcagga ggaatatgat tttgctaaag tagcgacttt
    12001 ctcctctcaa attgccttcg attacgtatt aaatacgtta ttctcagtga tatattctca
    12061 aaattttgaa gagaatatcc gcaacttgaa aaacaaacaa gaattgatgc aaaaaggcct
    12121 tcttaaagaa tatcggaaaa cttaagaaaa gatcctcatt tagtaataag aaagatagtg
    12181 tttgataggc tctctttgat atcatcataa aaacaggctg aagttttctt tttcaaacct
    12241 cagcctgttt gtttacggaa cgattcgaat agatatatta tagaaatgag gaatagctaa
    12301 cttctgtttc ttattttgaa cgattgacga aaaagatata taagaaggag gaaaatcagt
    12361 atacatgcag ctaacaatcc atacaggaaa aatcgtttgt tctcggatac tttctgtact
    12421 tctttttgaa cttcttttgc atgataagga acccggtgcc ctctgacaag taagcgatga
    12481 ctgttgacca tataaggtgt acaagttagg agtgtcacga aatcctgccc tttttcgatt
    12541 ctcagatctt ttgtatcagt cggttctacc gtcttgatct gatcgacttg ataagcgagt
    12601 gtccgtccat tgatttcgat atagaaagaa tcacctaaag tcagtttcgg taagtctgtg
    12661 aacaactttg cttgaggcag tcctcgatgg ctggaaataa cggcatgtgt attttctccc
    12721 cccgtaggat aagacgttcc ttccaataat gaggcacctt tttctagcag cacagtattc
    12781 gtttcatcaa aaattggcaa atggacattg atctcaggga tcgtcaaaat accgatcgta
    12841 tgtttctcaa aataggttct atcctttttc gcaggttctt ttttcttagt aaagggatct
    12901 gcacctggat ttccaccttc ttttgctaat tgctggtttt tctttgtcat gttttcttgg
    12961 attttagcca tgacttcttc attttctttg accgcttgtt gctgataatg agagatgatc
    13021 tgctgatcca gataattatt cagcgcgtcg ctaacaaatg gataagctag tatccctatt
    13081 cctgagagaa gcaagaaaag catcagcaga tctagaagtt tccttttctt tggtttcatt
    13141 tgtgttctcc ttgtgatgac tgtttgataa taaaagaaaa aggagcattt gcgtaatccg
    13201 ctgctccttt ttccaattct tgcaaaaaga ctaaatctga ctgtgcttgc gtctagcaaa
    13261 gtacagtcca gcaatcagta gaaggactac tcctgcaccg atatacacat agattccctt
    13321 accgcctgtt gaaggaagtg tacctttgtg tttatttggt attttttcag gagaaatcaa
    13381 ctgtcctgct gttacataag attgttcatc gatagtaaat gctacacggt ttgttaatgg
    13441 aacatatttt tctggcgctt tcgtttcttc cagatagtac gtgccatatt ttagacctgt
    13501 cacatcgatt aaaccgttac ttgtggttgt aaaaaccgtt gctgattctt tcgccgatac
    13561 ccagctgacg gcttttgtgg atgggtcgat cgataaatat ttcgctgtgt cactatcttg
    13621 atcacgaacg acgaattctg ctccagcaag tgtttggtct gatgtaacgt caccatctac
    13681 tttaacaaat cgtttgcccc cagtaacgac atcgactgac ggtggtgttt gatcatttgt
    13741 atgcccgtta tcgacatttg cttggttaga aaatcctttg gttggatctg ctttttcgtt
    13801 caaatgcata tagtaaacga atttcaatgt accgccagga gttaatgaag gaatataatt
    13861 cggatcaact gaaaccgtga atccgtctgt ttgctcagtg acagaataat ttactgggtc
    13921 gatttcttta tttccatcat ataaggcata agcatacgtt cctgaagaat cattatcaaa
    13981 tgttaaagca gcgtcatgag tatcgataag tttgaatgtt gtgtacttgt tttgcgttcc
    14041 ttctttatca gcgataccta atgggatatt gacagaaatc tcatattgaa ttttttcacc
    14101 gattgcgaca tcttttccat tcaattgagg tgttgtttta tcaactttag acgtatcatt
    14161 tttgatggtc ttttctactg gtgtttggct atttgccagg atctcaaaag gcgtgattgt
    14221 ttgcttttca atcatttccg cattatcagg agcttttact tcttctaaat tatattttcc
    14281 aacttctaaa tgattaacga tcaactggcc tttttcaata gatgcctcgg caaagtcatt
    14341 gttgccgatg tcataagaat gaccagtaat gaaatgtttg gctttggttt gatcagttgt
    14401 ccaagtgtac aatccatctg tgacactttg gatgtatttt ttgacgcttg gtgttccttc
    14461 ttctttagaa ataataaatt ctgctccatt tagtgcttcg ttttcggcag taccgatttt
    14521 tgtaactttc aacgttccat cattaccgac tgtattttta gggtagagat ggatagtatc
    14581 tagttcttct gtcccgtatt tataagagcc atctgcttgt ttgatcatct catatacagg
    14641 gaaagctaaa accatgtttg cggcagctga cactccgtct tttggttctt ctttgatcgt
    14701 gtagactgca tctttcccat tttgtttttt aggtaaagat aaagtgacat ttccatcagc
    14761 atctgtcgtt cctgaagcaa ctggtgtacc aggagtcaaa gactggactg cttgttttgc
    14821 tgcatccacg gacgctcctt tatctcgttg cgcataaaat tcttgagtga cgttataaac
    14881 tgaaaatgaa atatcggcta atccttggta ttgatcgaat tcgctcattt ctttcccgct
    14941 gttttggatt aaaggatcgg gtaaatcagt cattttcttt ttatgaagga tgacttgagc
    15001 tgctgtctcc tctgcaaaca ctttttttgc gccgaagctg tttgtgagta atggtaaaat
    15061 aaggaaaagg actcctaaca taacgtttat ttttttatga tttttcattt tttcactcct
    15121 gttctttttt tgataaacaa ccagattgca aagcttgcga ataccatacc aacaacgcct
    15181 aaaagtgtat ttttcgtttc gtttgttttt ggaagaaatg gacggctatt ttctttttta
    15241 ttgactactt tcaatatact gtccgcctct ttatcctcag aaagaaaagt tgaaatggta
    15301 aacggaattt ctgtttcact taaaatataa ccagaaggtg cttcgatctc ttttaatcga
    15361 taacttcctg ttttcaaccc ggaaattgaa aaagcgccat ttttatcgga aatcaggtga
    15421 agcgcatctg attcgttctt tgtccacttg tatccgttgg ctgtttgctg gaggtaattt
    15481 cctgctttgt ttttaataag gaatactgcg ccagctaatc gtttatctgg tttatttgtc
    15541 tcgattttga caaattgctt actccctgta tatacagaga ctttctttgt actgacacca
    15601 aaaccaaaat ccagtgttcc gttgttgttg atttccttgt tcgcctgtgc tgtactgctt
    15661 agctgcatct gataagaaat ggtcagtttt tttccagcat atttttccaa cgaagggatc
    15721 gaaaataaaa catcaaatcc gtgaggtttc ttgtgtatga caaagccttc tgtcagcttt
    15781 tctccattcg atgaaatcgt taaactatta ggtaaaaacg ttaatgcttc atccgcacta
    15841 tctgacaatt cgaacttttg atagtcaagg atatttacag gaattgtcgt atgcaaggaa
    15901 taagtgattt tttcaccaat cgtaaaatca tttctaggct cttctaatac cttctcaaaa
    15961 ggtggatcag ggtagtcgtt ttcttcattt ttaggataca aatggatagt tgaaagtttt
    16021 tgattgtttt gtccatgtac aggaagaaca actaccatat tctctgcctt ttctttgacg
    16081 acttctggta ctttggattc aatgaaaaga tacgctttat ctcttttttc ttgatcttta
    16141 gcggccagag aaaaagaagc gataccatct tcgttgttaa gagttgtagt tgttgcttct
    16201 gcggtaaaca taccggaaga cgcaccgttt tttgcgatct ctgcttgtgc ttcttctacc
    16261 gttttgccct tttcccgtag atggtaaaaa gaatctgtga catcataaac ttggaatgtg
    16321 acaccattta atcctcgata cgtttgtaat aaagcttttt cttgtccgtc atttggatga
    16381 tttttcggca gttgcccgtt tgggaaaagc aatttgtgta aagtaatctg aaccatttct
    16441 gtttctgttt ctgttgctac ctgagtaaaa gctggtttaa atagtaacaa gaagagacac
    16501 atactaagcc aaccaagttt tttcatattt agctgccccc tctttctttt ttcaaaaaaa
    16561 gataccaaat agaaaacgca caaccaatca tccctactag atagattcct aaacggccaa
    16621 ttcctcccgt ttcaggtaat ggtacttttg cctgattcgt gatgtctaaa gaaatctggt
    16681 tgtttttggc tcctggtgac agaacagatt catgatcttg tccatccact tgaattgacc
    16741 catcttcgtg tataactata gtaactggct cttttagacc ttggtatcct tctggtgtaa
    16801 aagtttcggt cagcgtataa gttccaggtt ttaaattttc gaatagaaag gtatctgttt
    16861 cttgtccatc ttttggcgat tctaagtcca tttctggtcc ctgcagtctg aattttgctc
    16921 cttttaatgt ctgtccgttg tcttcttttt tgtgtaccgt tagatcaaac gcttttagtc
    16981 gattttgatg agtgagcgtc catacattat ttgtcatctc ggtatttttt cccgtgtaaa
    17041 tgatttttcc atatttatcg atttggaatt cataatttcc cggcgaagta tcatatccgg
    17101 caggaccagc tgtttcaacg actcgatatt taccaggtgt gagtgaatca aaactggcaa
    17161 gacctttttc attcgttgtt tggctgtcaa ttggctgata agtaccattt gcttcttttt
    17221 tctgtaggga aaatgctgct cctttaagat ttatctcttt gtccgtccct tgcatcgcat
    17281 acttacgtac tgctacagga acttcaacaa aaggattttc aatagtcaac tggatcgtat
    17341 catcggaagt agtgactgtt tttccatcaa tggttaccgt accatcagaa gcgatcttga
    17401 tttcccaagt agtctttttg cttaatccat gcccttctgg agcttttgtt tctgtcagcg
    17461 tataggtcat ttctttttgc aatttgacat tttctggaag agaataggtt ccgtcgccat
    17521 gatctgttag taaagtatca atagaatctc ctgttaattt gaaaacagcg ccaataagat
    17581 ccttttcacc tgtagaggaa tttttcgtta ttttcaagtt taacggtgtg aattgtttag
    17641 tatttttcca tgtcattgca tctatttgtt gagaatcgta tcctggtaca ggtaattctt
    17701 tgattgtttg gtaactgaat gcttgacctt gattattgta ttgaggtagt gataatatct
    17761 cttgataact ttctccgcta tttgcagata atttgtcaat gtctgcacgt tcccacgtat
    17821 ttgttgtatc tttagctggt ttaatgattc gaatatatcc gtttttccaa gctgcagcat
    17881 ttgtcgtatg ttcccgttga atctcaaaag taacttgatc tggacgatca gctagattgt
    17941 tgtcaaattc ttcccataac tttttgatgt gaagactgac tccgggagct ttagcagatg
    18001 gtataccgaa ttcagctaat tcattggtat caatgtttgg ctggaaagtt gtcctgccgt
    18061 tcatttgata ccagaaattt ggatggaagt cctcattttc tgtttggatt ctcacttggt
    18121 aatggatttg gatttcttgg tcttttccta aatagatctg attgctcttg atggtatttc
    18181 cttctatgga aatagatgga gtgaccgttg taggacttgt ccccacactc ttgactgaaa
    18241 gtgtaccagg ctgataaatg aatggttctg cgattggatc atttatttgt ccattgctta
    18301 cagttgctga gatctgtaca gcttttttgg ctaggtattc agagatatct gttgcatgat
    18361 cagctgattc atagtaaaga tcgccttttt catctgatga aaccatttgt cgcatacgag
    18421 actctacttc tgcttttgag agaccagctg ccggatcgct ttgaagttgg atgccaagac
    18481 catgtatttc gattcctcgt tctttgagtg ccatcgcttc tccgatggtt gcgataaacg
    18541 tactgtcgat tcttctggat agattgtttt ggtcaggtgc atcatagatt cttgagatta
    18601 gagaagtatt tcccggccga tcttgcgtat tagaaaactg agttccgtaa taattgctgc
    18661 ttgattgtgc gtgtacccgc tgtactttat aggaaaatgt tggtacacca tccgtcagca
    18721 aaacgatcac ttttttatgt ccatttggaa cggatagcat gcttcctgca tctcttagtg
    18781 ctttttgtgt aaaagtacca ccatttgtcc gtgaaggtgt aatggatttt acttgatttt
    18841 tcactgaatc aaatgaaccc atctgtactg caccgttact gtagctatat ccttcgcttg
    18901 agtagccgac atatcccata ttgattttgt ctgtgatacc gctatctgct aaagtatcga
    18961 caaaacgatc gacaccaatc tttacttcac cgatccgatt attgtcgttc atacttcctg
    19021 accagtctac gaccaatacg agatcaagag gcgtgatatc tttttgtaca tttccacgag
    19081 cattcaaata aacatcaaac aatccgggtg tacttgtttc ctttgcatac tttcgtatcg
    19141 catagtctgc ttgattaccg gttcctccat attcgatata cgaatgggtc cgatcattag
    19201 gatctccatt ccaagaattt ataccatccc attgatttgt tccgttttta ttgccttgat
    19261 gatcaagaac atttgtattt cctgtaggtt gccaactggc tttaggataa gtgccctgat
    19321 catctgttgt atattctgta ggaatcgttt gattagatgg cacactcaca gctggcaagg
    19381 ataatgtagg caatgtaaat gggctgacag aattttcgtt ttcttttgat gattcttctt
    19441 ctatttcgtc tggctttggt actgacacat ctgtatcttt cttattttct gtctcatcaa
    19501 taatgtcata ttctttatta tcgacgatca gcttgtttgc ctggctgtgg taaagtgctg
    19561 gatcgattgg aaaactcaga tcgatagttt gtttagtgtt tgatgagatt tccaacgtaa
    19621 ttttcccgct atttacctga taagcatagc catctccttc ttttctttcc acatcactta
    19681 agtaattttc gactagacca atttcttttt ctatcttttc agcactggtg ttattcgcat
    19741 ttagcttgat gttcagtgct ttttttccag actgatccga aatgacttca gctgaaattg
    19801 tttctaaaac tgttggatca tctgtagtgt ctgctattgc agataatggc acaaagtttg
    19861 ataaactcaa tattatcagc atgaaaataa ctttcagttt cttccctgtg gtcgtcattt
    19921 ttcccctcct ttttcttttt ttctgttttg acgtgaatag tgaaaattat taaaataaac
    19981 aggcgttaca taattttatt tattttaata tgataattat tatacgaagg gcgctaaagg
    20041 gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc ttcgattaat
    20101 gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg ttgttggaat
    20161 taaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac aagtggatga
    20221 ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta aggtatatcg
    20281 aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa aaattggggt
    20341 atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa atgtcgcaaa
    20401 gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa acgattgagt
    20461 attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg aaacaatgca
    20521 atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt cagttaacta
    20581 gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta agttactacg
    20641 gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt atggtcaaga
    20701 ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct ttctttatta
    20761 tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa tggaaagctg
    20821 gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc ttgaaaaatc
    20881 tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt gtcaatacga
    20941 ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata cgaaaaaata
    21001 tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa tatgttgttc
    21061 aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga
    21121 cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg
    21181 gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt
    21241 tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg
    21301 ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat
    21361 atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag
    21421 atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt
    21481 tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag
    21541 aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg
    21601 ataaaaatgg ctcttcgtca aattgtgttg gcgaagacaa atttgaataa aacttagtcc
    21661 gatatgcggc gcgttctgtg cgccgcatat ttttttagtt ttcaaagatc agaccttgtt
    21721 gtaaatagat ccgtgcccga aagttataaa agttgcgata accaaacgca attcgtttaa
    21781 tcagcttgat cttgttattc agcccctcag tgacgccgtt tgagtggggc accttaaagg
    21841 catttgaaat acccgattgg tagcgatgga agatcgtgaa tttcttttta aacgactgag
    21901 gtaacgtaga tgaaattgtt ttaagggttt cttgaaagcc ttccgtatct cgtttaacga
    21961 aataatagcg taataattga caaaaatgat acgcagttcg caattcttcg ttgtagctta
    22021 acagctcatc aacgatttct gtttgactaa ttggtcgttt aaacagtcga tgataatgcc
    22081 gtttgagtgg ctccaatgtc gtggaatctt tgagtaagag tttccaatat cgcttgagcc
    22141 gacggtactt cttctgattt tcagagtgtg aggttcgaaa acgattcatg atctggacac
    22201 gtaattgatt gaagctgcga ttgatgtgtt ggacaatatg aaagcggtca gttacgatct
    22261 ccgcatttgg aaagactgtt ttaagcaact gacaatagct ggcattcatg tccatcacta
    22321 ggtacttgac ctttaaacgg gcctttctag gaaagcgcag gaagtattga gttagtttgt
    22381 aaagtctgcg atcttcaagg atcgtaaata gctgattggt tgtcccatca acacaaataa
    22441 aactcatgga accagaaaca gacttcattg atttgaactc atccatacac aacaccgtcg
    22501 gaaggaagtt catacgtggc tggtaatgct tcgttgcttc acgcaagatc cgtgtcaccg
    22561 tcccatccga gacgaagtac ttcttagcga ttagtttacg tgattgattt tcgtacagtt
    22621 ccatgagaat cgcctgcttc agttcctttg ttagttgatg atgttcatca actaagtccg
    22681 ttttcgcaga aaaggtccgt ccacaatcat ggcagagaaa acgggtacgt tttaattcta
    22741 aaatagtcaa cttttctttg actttcaaca tctgagtgcg tgtcgtatag gaaccgttgc
    22801 ggataatacg aatcgatcca cagtgttcac aatgggaagg tgagtcatcc aatctccctt
    22861 taataaactg cgcttgaacg cctttatatt ctttttcaat cagccattct tcatcaaaaa
    22921 ataaatgttt atctgttaat ccgagtaatt ttctagtatg attatccatt gagagactcc
    22981 tttggtaggg tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt
    23041 gaaacgaaga aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa cacaaaatat
    23101 tatagaacca taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct
    23161 tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta
    23221 tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta
    23281 actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt
    23341 tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc
    23401 tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac
    23461 tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag
    23521 atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa
    23581 tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag
    23641 actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc
    23701 tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta
    23761 tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc
    23821 ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg
    23881 gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa
    23941 tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat
    24001 caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga
    24061 aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc
    24121 gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt
    24181 taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg
    24241 taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc
    24301 tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc
    24361 agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa
    24421 agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat
    24481 ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt
    24541 atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat
    24601 tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca
    24661 aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat
    24721 ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc
    24781 tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag
    24841 tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat
    24901 gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa
    24961 tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca
    25021 tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa
    25081 gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta
    25141 tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt
    25201 ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa
    25261 actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct
    25321 ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact
    25381 tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta
    25441 aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag
    25501 aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct
    25561 agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa
    25621 tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg
    25681 tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat
    25741 ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact
    25801 attagtagta gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat
    25861 cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt
    25921 ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc
    25981 aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt
    26041 ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta
    26101 tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga
    26161 tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta
    26221 taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca
    26281 aaataaggga atcattttga caaaagagca gatatttgac cggatctggg gatttgattc
    26341 tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag
    26401 tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt
    26461 gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg
    26521 tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc
    26581 aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag
    26641 tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg
    26701 aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca
    26761 ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc
    26821 gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata
    26881 ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag
    26941 cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca
    27001 agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc
    27061 tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt
    27121 ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga
    27181 ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat
    27241 ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt
    27301 tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata
    27361 cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa
    27421 aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc
    27481 atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga
    27541 tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc
    27601 cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat
    27661 ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg
    27721 aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag
    27781 tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa
    27841 atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga
    27901 aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta
    27961 cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc
    28021 tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac
    28081 ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt
    28141 aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt
    28201 gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac
    28261 cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt
    28321 ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt
    28381 cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt
    28441 catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc
    28501 tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg
    28561 tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa
    28621 ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt
    28681 ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc
    28741 ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa
    28801 tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc
    28861 ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat
    28921 gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta
    28981 tattgttact tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg
    29041 gaatttgatt aaaacaattt aattgaaatt tgaaaacatt tatgttaatg aaagtcatat
    29101 tctaataaaa tgtcatttgt ttctatatga atttttatga aaaatttcat ataatttcca
    29161 ctcgttgtga tggtttaatt tatccaatcc atcaataagc aaacaactta tataaaaaag
    29221 gattaattct caatataatt gctcctacca tagaataaat cttcatgtaa agtaatgaag
    29281 taccaactat ttctaatact tctttaagaa ttatcccaac aatatcgtta gacaaaccat
    29341 tattgaataa atggagagcc aatcgattct gaaaaaatgt tttctaattt ttcggaaata
    29401 aaagaaagag cctcctgcca ttagaacgaa ccaaatccat ttcattgctc atgaagtaac
    29461 cacatagcta aaaagaggat aaatcaatta aaagatcaaa agaatgactg cactaataaa
    29521 ataactaaaa atgtatcttc ttacttcata tcttttagct agtacttagt taaaaatttg
    29581 cttcctaaga tcaagacaaa ctgtctatct tcatttgatg aaatgcggta aaatatagaa
    29641 aatgaacact attggaggga atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa
    29701 aagttaaagc aacgtacaca cttgctccct tccgtctttc aacagttttt agaagaaaat
    29761 cgttcttccc tttcattagg cagtcgctat gaatacgcaa aagattttca tctgtttact
    29821 gattatttga gaaaagtatt ttctgaagaa gtggatgatc agctgattat ccatttagaa
    29881 aaacaagatt atcagaattt cttagctaaa atctatcgac atactcgttc gttccaaact
    29941 acagcgaacc aagaaacgga gcaattgttt gaaaatagct attacgggat ttcaaggaag
    30001 caatattcat taaatcattt tttacgattt ttgtatcaaa aaggactgat tgaagaagag
    30061 atttctcttt tgggtacaag tttaccggaa acaaccaaag cagctccgcg atatatcgat
    30121 gcagaacttt tcaaagattt tgagcatttg tttatcccga ttgaaggctt tcaaacaagc
    30181 agagaagcta gctttgaaga aaagaataga ccgaggaacc ttgcaattac tgccattctt
    30241 ctgtattgcg gattaaagat ccatgaagta gtagaactta aacgtaaaga tgtatctttg
    30301 accaagcaga ttcttaatct gaatagaaaa gaaaacagac tgaataaagt accaatacca
    30361 aaagaagcga tgccatttct ggagaactat tttaagttaa cagcacaaaa tacggacgaa
    30421 aattcttttg tttttcgttc ctcacatgat cagcaaattt ctcctcggac tattcgccaa
    30481 attttgagca aaattacagt atataaaaac gtatcgccag aactatgcag aaaaacatat
    30541 cgttattatg cagaactata tttagatgat gctgacgcgg taaattattt atgtggtaat
    30601 cgatacctct catcccattc ttttcaagat atctgggaaa agatggttga tttttcttat
    30661 cacgaattag cggcattcgt tcgggtttct tccatataaa acaaatgtat gatctttttt
    30721 tcacaataaa ctatgttatt atataaagga ttatgttacg attatcttga atttgtatat
    30781 tacttaacaa aagataatgg agtgtgaatg atggagacca taacgtattc ccctttgaac
    30841 ttatttacga attttcaaga agctgcagct aacttttccg agaccccgat tatctttgat
    30901 gagccacttt cagctttccc agaattagga cttgaaacaa cttacaaaaa atgctgcgaa
    30961 agtgtgttga aacgggctta tcaattggct catcttggag tgaaatccgg agacaaagtt
    31021 attatttata agagttccgc ttttgacaca taccttctag ctgtttctgt gtcttacctt
    31081 ggtgcagtgc ctgtcatgac ttcttatcat ctaccaacaa caacaatgga agtatttatc
    31141 gatcgtttag aagacccttt cattttattt gatgatgaga caaaaaagcg agtgagagaa
    31201 atttcaaatg gaacaaaatc aaaacagatt cctattcttt atttaatgga acaacctgct
    31261 ttgtctgttt ctcaatcatt tttagacaaa aatgaaattt cttatatgac ccatacttct
    31321 ggtacaactg gtatccctaa acttatttgt cattctgctc attctatggg atggcggacg
    31381 aaatggcaaa aaacagtttt cacaaaaatt tcggaaaaga aattaatcgc ttttcacatc
    31441 tctccagtgc attccagatt caatattggt atctcttctt tgatgtccat gggatttcct
    31501 atgatgcctt tagcaaacgc acaaagttca aaagttgttc atatgttgga agctcaccgg
    31561 ccaattgcac tagagaccca tccgaataac tttgttcagt ggcgttttac tgctaaggag
    31621 catccagaag ctttttcggg tatccgatac tatcactcca cttttgatgc tataaacaac
    31681 cagacaatga agacattttt gcgaacgtcg atggcacagg atgcggtatt tcttcaagta
    31741 tatggccaaa gtgaatgcgg accaatgatt cttaaggctc atacactaga gagtttgaaa
    31801 acaagtgatg caagagatat gggcgttgga ctggaagaca tgacaagcgc tcggattaca
    31861 gattcagttg ggaatgtcct acctgaaaac acagatggtc atatacaatt actgtctaaa
    31921 ggacgggctt tgacttatta caaggaagat gcacggttcc aggaaaatgt ttatggcgat
    31981 tggtgggata gcggagatta cggttttatg gataagcatg gccacctatt tttgaaagat
    32041 cggcaagtgg atttgatcga aacgatcaat agtacattag ctattgaaga ctttttatta
    32101 gattcgttag attttttggc agaagtagtg attgtaaggg ataaaaatca ttctccacaa
    32161 ccgattattg ctttagctcc tgataaagaa atggattgga atcgatggtg ggagcaagtc
    32221 catgaacttc ctcgcttgaa cgtccctatc atccgtgatt ttgatgcaat cccgcgaaca
    32281 gctacaatga aagtgcaacg gcttcaaatc gagaaagaat taaaatcaca aaagtcctaa
    32341 ctatttaagg aaagaaagat ccattttggt ctttcttttc tttttgtttt tttaaattgg
    32401 atgagataaa aatatgccaa ttggctgttt ttcttttttg aaaatttcgt tatactctta
    32461 ttcatggagg aaaatgttat gaaaagaatc ttgacaattg gcggttctga tccatttgct
    32521 ggtggaggaa tccaaacaga tttaaaaact tttgagaatt ttggtctatt cggactaagc
    32581 gccttgactt ctataggggc actagatatt aatgagcgat ttatgctgga atctatatct
    32641 ttggaattgc ttaaacgaca gttagtttcg attgataaaa tgacttcatt agatggtatc
    32701 aaaattgggt tattgaattc aagtgaagca atcatggttg ttcgagactt cataaaaaca
    32761 aaacaaaata tcccaatcat tttagaccct gttttggcat tcaaagaaac tgaagctgtg
    32821 atcaatcaat tgtatatttc gcagctcatc gaagaacttt ttccacttgt tacgatgatt
    32881 acgcctaatt tggcagaagc tgctttactt tctggtcaag ctcttccctc ttcgttaaca
    32941 gaaatgatcc atctcgctaa agaactcact gcttcaggaa caaaaaacat tgtgatcaaa
    33001 ggcggcacaa ggatggctgg agatgaagct gtggatcttc tctattcaat tgatcattat
    33061 gaggtatttt ctcataaaaa aatcgacact acaacagtaa acggggccgg ttgtgccttt
    33121 tcttctgcaa taacagcgaa tcttgtgtta ggggcagatt ttaccaaagc agtagcagaa
    33181 agtaaaaaat ttgttgccca ttgtatttct aatggcgtgc tgatgaacga tcataccgga
    33241 agtgtctggt ctggcggtat tcaaaaagga ggaatgagaa atgaaaacta agcaattaac
    33301 gatctatgct atgctgattg cgttgacagt tgccctttca ttaacaattt tgatccctgt
    33361 accggctacg aatggatttg tgacattatg cgaggccggg atctataccg ctgcttttct
    33421 ttatggtcca acgggtggat tagttgttgg tgcttccagt ggtctattga tcgatttgat
    33481 ttcaggatat ccgcagtggg cagttttttc attagtgatc cacgggttac aaggatatgt
    33541 tgctggttct ttcagcaaat cttctactcg tatgtgggga ttagggcttt tattaggaag
    33601 cttagttatg atcatcggct atggtatcgc cggttggatt ttatatgatt ggccggctgg
    33661 agtggcttct ataccaggaa acatcttcca aaatctgatt ggaatgggac ttgcctttcc
    33721 tataactgca agtcttcaac gtattcaagc acggaagcaa tattaattga aaaagaaggt
    33781 tttagaaaat gaagttgtca gaaaaagaat taaaagacca attgactgaa atagttaatg
    33841 acatattggc tgaggcacac ttgaaaaaag gtgatctttt tgttttagga tgcagcacaa
    33901 gcgaagtcgt tggaggacat attggaaaaa attccagtgc ggaagttggt caatggatca
    33961 tccgtacgtt aaaagagttg ttggatccta aagaaattgc gctagctgtc caaggttgtg
    34021 aacatttgaa ccgagcatta gtagtagaac gaacagtggc agaagcaaaa aatttcgaaa
    34081 ttgtctctgt tgttcctgca ctacatgctg gaggtgcttg ttctgttgct gcttttgacc
    34141 agttcaatga tcctgtcgaa gtggaacacg tagttggaca agccggtatc gatatcggtg
    34201 acacatcaat cggtatgcat atcaagcatg tccaagtacc tgtcagacct cgtttgaaaa
    34261 cccttggaca agctcacgtg acagcccttc gctctcgtcc taaatatatc ggtggtccta
    34321 gagcgaatta ttgaaatgag taattgacag agcaatctga aaaacttcct cgcaaagtcc
    34381 aactaatgaa ctttaacgag gaagttttac ttgtttgttt tatataaccg agcttcataa
    34441 ggtgctagta tgcctttagc ttcaaattct ccgctagagg aatttgacaa gagtaatgtc
    34501 caggactgat ctgcttcttt tggaagttca tagtcagcaa gtgattctgt taagtttacc
    34561 ataataagat attgttcatc ttctaaatat ctttcataga cataaagttg tggatgttca
    34621 tgaagatatt ctttataaga accgtatatc agacctttat tggtttgtct aatgcggatc
    34681 atgttcttat agaattgcag gaccgactct ggttcatatt cttgttgttc tacattgatc
    34741 gttttggtat ttggattagt gatcagccaa ggttcatgtg tagaaaaacc agcatattgt
    34801 tccgaagtcc attgcatggg tgtcctgctg ttgtctctcg ttgtttcacg aatgatatcc
    34861 aatgcttctt ctcttgtctt accttccgca agcagccttt tataaagtct tttcgaatct
    34921 actgcatcaa cttgatcgat cgattcaaag ggcatattcg tcattccgat ttcctgtcct
    34981 tgataaataa atggcgtacc ttgcaaaaag aaatacatca tagcgattgc ttttgcagac
    35041 atagcccaaa aatcgggacg agtatctcca aacactgaaa cggcacgagg aacatcatgg
    35101 ttttccatat ataaagcgtt ccatccgata ccatccaatg aagtttgcca agcgcttaat
    35161 gcatgtttca atttaaggac atctaattgc ccttcttttt cttgctgcca gatatggata
    35221 tgatcaaatt cgaatatcat atcgaaataa ccgtctttcc ctacccattg tggtccttct
    35281 tctgcagtga caccacttgc ttctccgaca gtcatgatat cgtactcttc aaaaacatgt
    35341 ttcaaatcgg tcaaatgttc ttcgattcct gagacatttt gaaagggtga gaaagggttc
    35401 tctgtggctt ttactgaata ttcatctttt tttacatgag aaatcgcatc taaacgaaat
    35461 ccatctatcc ctttatccaa ccaccatcgg atcatattga atagttcttc tttcaatttc
    35521 tcgctttccc aatttaagtc tggttgttct tttgcaaaaa catgcagata atactgtcca
    35581 gtttcatgag aatactccca agcagaaccg ccgaaaatcg atgtccaatt attcggttct
    35641 ttgccatcta ccccgtcgac ccaaatgtaa taatcgcgat acgggctttc tttagatttt
    35701 ttcgattctt caaaccattg atgctgatcc gaactatggt tgatgacaag atccataatc
    35761 actttgatcc ctaattcatg tgcttctttt agcaataaat caaaatcttc catcgttccg
    35821 aatctctctt ctatatcttg atagtcactt acgtcataac cattatcgac aaatggagat
    35881 tgatagatcg gattgatcca gataaagccg ataccaagat ctttcaaata attaagtttt
    35941 tcacggatcc cattgatgtc tccttgacca tcgccattgc tatctttgaa actagcagga
    36001 tagatttgat agcctactgc ttctttccac catttttgtt cttgtatcat atcgtttcac
    36061 tcctttaacg gtccctcttg attataaccg attctatttt agaagtatcc ttgttaccgg
    36121 taactagaaa cgaaaagctc aacgaatctg ctaagtgcgt tcgttgagct ttattttatc
    36181 atagatagcg ggcaatcagt tgaatcattt gcttgggaaa ttcattcaag tcagtaatat
    36241 ctacaaaacg actttcaccg tagatcgact tgatttcttc tttgtcttgg ccaatagctg
    36301 cggagacaaa taggattcct tgacgttcat attctttcaa aacagactgg atatcttctt
    36361 ttgctttttt gcctgtataa tccggcaacg cttttggctg accgtcacta atattaagca
    36421 acagtttggt tgttgcggat tgtctgttta atttttctgc tgctaaacga agtgcagcac
    36481 catcacgatt gttttgcctt ggtttcatag tgactaattt ttcatttaac cattgaaatc
    36541 cgtcttcaaa ttctttataa gaaaacaaag aagtcttttc tcttgacgaa cggtctgctg
    36601 tatctccgta aatcaacaat gggatatcga ctttcttaga aaattcggca atggcaatcg
    36661 ctgctttttt tgccgcttcg atccgatcat cccgtatcat tgacccagat tcatcgatcc
    36721 gtacagctac cgctaaagaa ggttgctcat gtggcgggtt ctttttgtca aaatttctca
    36781 aatcaccata tgctactttg cttgcattga accgctgacc ttcgtatttt cctttttggt
    36841 acgtctctgt ttgttcatta tccagtaact ctaaaatttg ccggctaagt gattctacta
    36901 ttggcattac ttgcatgtgt agctcacgtg cttccggcgt ttttccttgg aaagctggtc
    36961 ggtgaacgat gagaccttct ttttcatgaa tcgtttcctt catgatttgt cgcgcttctt
    37021 tattcagttg cttcttgatt tccttatcat gtgcttccat gtcagaagca gacatttctt
    37081 gagaatcatc tcctgtttgt tgttcttttt gattttttcc ttcgatatcg ctactgcgtt
    37141 ctttttgcat ttgaggagga atctcgtttt tttctattgt ttcttcttgt ttctcttctc
    37201 cctcatttgg ttcttcagca gacaacgatt tcttttcatc taagcgcaga atttcttcag
    37261 cattttcagt gatgtttcgt tcatttgctt cttcggcgct aatggttctt tcttttgtat
    37321 gctcagattg aggatgaccc catttttctt ttctcaatat gtcctgaagt tccagtagca
    37381 atccactgtc agacaaagct tgttcaagaa ttgccaattc atctgaatca gtggataatt
    37441 tatacaaaac ttttggataa atcgactgga gtaaatcttg tccttgctta taggtatttg
    37501 cccaataaaa ataagaacgc attcctgcta cacctttgat ggcgttagct tttgcagttt
    37561 catccaacaa acggataatc tctgccatct ttaagagaag ttcttgtttt tggatcatag
    37621 ttttcgccat cgcacgttct gccatgactt ctaaggcagg aagatccatt ttttccgcat
    37681 gttgcatccg atctcttaaa gattcattta atgggcgatt tccttggtaa tttcgatttg
    37741 tagtgatcac aatgacgcaa tctggatggc gacgaatgat tcctgtcggt aaattcaaca
    37801 tcccatttgg ttcgagcgct gagttaagtg ccacaagaac agaggcatct cggatgacag
    37861 tcggctcttg aatctctaat aaatagcctt tttcaatcgc tcgaacgatt tcagaagggt
    37921 aatagctgta atggattgca tctggataat cagcttcgat cctttgaact agctctgcta
    37981 atttttcttt agcagaaaaa ggatcaatgc cgtaatggtt ctggatcagt gtcagtacag
    38041 cttcaagtgt atctgtttgg taaatagctt ctagtaattc ttggtcttgt tccttattag
    38101 aatctactac aggtaataag gctccgaaaa catctgattt gtccatatct gcaaaacagg
    38161 tcacttttgt atagggaagt tgcagatcag cagatagtgc tttggctaat tgtgtcttcc
    38221 ccgacccggc atctccttcc aaaagtacgt tactgatttt catttcagga tcttgccaat
    38281 tccctttgat ttcggaggcg atccgccatt cttctgcact tgtccgatgg cttttcggtt
    38341 tcttccagat catttgcttt tctagccgtg aaaaggttcg gttttctgtt agtgagaaat
    38401 tctccatgct tctcttcccc ttttgatcat ctctgtctat taatatccta gctcatctaa
    38461 aagacgtttt aaaatacgga atcgttcccc aatcagttct ccgtcttttt gtagtgtatc
    38521 gtaatacaaa atgatgtctt catcgatctt tggatttttg gtatctactt ctaccgtcat
    38581 tcggttccct tgtaatccaa tgatgtctac gactggattt tctggatcat aaaatttttc
    38641 ataacgataa gcatcttgga agtataaact tccttgcgca gctaagatcg ctaaatctaa
    38701 aatacgtgtg attggtaatt cttccgactg tcgtgaccat ttttctccag tatgtcgcca
    38761 taccttgcct gagatatcga cttttccacg atcattccac tgagcaaggc ccagtgaaag
    38821 cccttttgca tccatattta atgcatttct tccatcaact ttatcatagt cttctacaac
    38881 gattactggt ttatgtttta aatttgttgg tatttccatt attttaactc ctcatccttt
    38941 tatttttagt aaataagtaa attagtagtt tactcattta ctaaactata tgcgacatta
    39001 tagaggattg aagtgaggat tgcaaagaaa aagagcttca ttgatagaaa taaaaacacc
    39061 atctcgttct tttacacgct cattagtaac atgtagaaag tccaagatga tgttggaata
    39121 ttttggctct atactttttg atgttggtga acttcaccaa catctttttt gt
//
LOCUS       DAFCHQ010000015        37138 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000015 DAFCHQ010000000
VERSION     DAFCHQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.015"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..386)
                     /locus_tag="LNC71_000892"
     CDS             complement(<1..386)
                     /locus_tag="LNC71_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6465983.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKIL"
     gene            complement(571..969)
                     /locus_tag="LNC71_000893"
     CDS             complement(571..969)
                     /locus_tag="LNC71_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBK6465984.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(966..1739)
                     /locus_tag="LNC71_000894"
     CDS             complement(966..1739)
                     /locus_tag="LNC71_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK6465985.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1736..2419)
                     /locus_tag="LNC71_000895"
     CDS             complement(1736..2419)
                     /locus_tag="LNC71_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBK6465986.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2586..3473)
                     /locus_tag="LNC71_000896"
     CDS             complement(2586..3473)
                     /locus_tag="LNC71_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6465987.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3470..4339)
                     /locus_tag="LNC71_000897"
     CDS             complement(3470..4339)
                     /locus_tag="LNC71_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6465988.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4476..5762)
                     /locus_tag="LNC71_000898"
     CDS             complement(4476..5762)
                     /locus_tag="LNC71_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6465989.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6035..6613
                     /locus_tag="LNC71_000899"
     CDS             6035..6613
                     /locus_tag="LNC71_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6465990.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6613..7542
                     /locus_tag="LNC71_000900"
     CDS             6613..7542
                     /locus_tag="LNC71_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6465991.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7539..9275
                     /locus_tag="LNC71_000901"
     CDS             7539..9275
                     /locus_tag="LNC71_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK6465992.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9338..10174)
                     /gene="pheA"
                     /locus_tag="LNC71_000902"
     CDS             complement(9338..10174)
                     /gene="pheA"
                     /locus_tag="LNC71_000902"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBK6465993.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10171..10680)
                     /locus_tag="LNC71_000903"
     CDS             complement(10171..10680)
                     /locus_tag="LNC71_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBK6465994.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10673..11968)
                     /gene="aroA"
                     /locus_tag="LNC71_000904"
     CDS             complement(10673..11968)
                     /gene="aroA"
                     /locus_tag="LNC71_000904"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK6465995.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11981..13072)
                     /locus_tag="LNC71_000905"
     CDS             complement(11981..13072)
                     /locus_tag="LNC71_000905"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBK6465996.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13089..14255)
                     /gene="aroC"
                     /locus_tag="LNC71_000906"
     CDS             complement(13089..14255)
                     /gene="aroC"
                     /locus_tag="LNC71_000906"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBK6465997.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14257..15321)
                     /gene="aroB"
                     /locus_tag="LNC71_000907"
     CDS             complement(14257..15321)
                     /gene="aroB"
                     /locus_tag="LNC71_000907"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBK6465998.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15354..16376)
                     /gene="aroF"
                     /locus_tag="LNC71_000908"
     CDS             complement(15354..16376)
                     /gene="aroF"
                     /locus_tag="LNC71_000908"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBK6465999.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17035..18009)
                     /locus_tag="LNC71_000909"
     CDS             complement(17035..18009)
                     /locus_tag="LNC71_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBK6466000.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17990..19873)
                     /locus_tag="LNC71_000910"
     CDS             complement(17990..19873)
                     /locus_tag="LNC71_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBK6466001.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19891..22116)
                     /locus_tag="LNC71_000911"
     CDS             complement(19891..22116)
                     /locus_tag="LNC71_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK6466002.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22231..23265
                     /locus_tag="LNC71_000912"
     CDS             22231..23265
                     /locus_tag="LNC71_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6466003.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23332..24345)
                     /locus_tag="LNC71_000913"
     CDS             complement(23332..24345)
                     /locus_tag="LNC71_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6466004.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24423..24611)
                     /locus_tag="LNC71_000914"
     CDS             complement(24423..24611)
                     /locus_tag="LNC71_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBK6466005.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25051..25179)
                     /locus_tag="LNC71_000915"
     CDS             complement(25051..25179)
                     /locus_tag="LNC71_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBK6466006.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25182..26555)
                     /gene="rlmD"
                     /locus_tag="LNC71_000916"
     CDS             complement(25182..26555)
                     /gene="rlmD"
                     /locus_tag="LNC71_000916"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK6466007.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26666..27721)
                     /locus_tag="LNC71_000917"
     CDS             complement(26666..27721)
                     /locus_tag="LNC71_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK6466008.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27731..29161)
                     /gene="gatB"
                     /locus_tag="LNC71_000918"
     CDS             complement(27731..29161)
                     /gene="gatB"
                     /locus_tag="LNC71_000918"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBK6466009.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29158..30630)
                     /gene="gatA"
                     /locus_tag="LNC71_000919"
     CDS             complement(29158..30630)
                     /gene="gatA"
                     /locus_tag="LNC71_000919"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBK6466010.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30630..30935)
                     /gene="gatC"
                     /locus_tag="LNC71_000920"
     CDS             complement(30630..30935)
                     /gene="gatC"
                     /locus_tag="LNC71_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBK6466011.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31041..33077)
                     /gene="ligA"
                     /locus_tag="LNC71_000921"
     CDS             complement(31041..33077)
                     /gene="ligA"
                     /locus_tag="LNC71_000921"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBK6466012.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33094..35328)
                     /gene="pcrA"
                     /locus_tag="LNC71_000922"
     CDS             complement(33094..35328)
                     /gene="pcrA"
                     /locus_tag="LNC71_000922"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBK6466013.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35483..37042
                     /locus_tag="LNC71_000923"
     CDS             35483..37042
                     /locus_tag="LNC71_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBK6466014.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10264 a         8064 c         6697 g        12113 t
ORIGIN      
        1 agaatctttt gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc
       61 atttgttttt gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt
      121 aaaccttctg gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata
      181 taatctttta ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct
      241 tctgcttgat agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc
      301 agcaatgttt tattttcctg caattgaaca tatagaaatg tagatcgctc aatcagagta
      361 tcttttaaaa caactaaaaa attcattttt ctcccccttt tttcatcatt tttaaaatag
      421 catattttga aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca
      481 taagtattca cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg
      541 tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt
      601 ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc
      661 aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat
      721 gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac
      781 aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga
      841 acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac
      901 atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag
      961 atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca
     1021 tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc
     1081 attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt
     1141 gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta
     1201 tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg
     1261 tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat
     1321 cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc
     1381 ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct
     1441 actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca
     1501 atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa
     1561 aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg
     1621 cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc
     1681 aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt
     1741 ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca
     1801 ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa
     1861 gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa
     1921 tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag
     1981 ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata
     2041 ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata
     2101 tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc
     2161 cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc
     2221 ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc
     2281 cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg
     2341 caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt
     2401 ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa
     2461 agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca
     2521 cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg
     2581 tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat
     2641 acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt
     2701 gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc
     2761 aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc
     2821 gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt
     2881 agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga
     2941 aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg
     3001 attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc
     3061 gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt
     3121 tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc
     3181 accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac
     3241 aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc
     3301 acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat
     3361 aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct
     3421 tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt
     3481 ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg
     3541 aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt
     3601 gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa
     3661 aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat
     3721 ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt
     3781 cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag
     3841 tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc
     3901 agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt
     3961 gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa
     4021 agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa
     4081 taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa
     4141 aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa
     4201 atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa
     4261 taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga
     4321 ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa
     4381 gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga
     4441 tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact
     4501 tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga
     4561 gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt
     4621 tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct
     4681 tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc
     4741 gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct
     4801 gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct
     4861 tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat
     4921 tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact
     4981 gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct
     5041 tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc
     5101 gtgactgtct ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt
     5161 actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca
     5221 tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa
     5281 cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga
     5341 acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct
     5401 tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt
     5461 atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa
     5521 gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt
     5581 tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc
     5641 cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct
     5701 gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc
     5761 atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca
     5821 aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa
     5881 aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa
     5941 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt
     6001 aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga
     6061 tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt
     6121 tcaattaata gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc
     6181 aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg
     6241 gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt
     6301 tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt
     6361 gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc
     6421 caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat
     6481 tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg
     6541 ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc
     6601 acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg
     6661 gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa
     6721 aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc
     6781 caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat
     6841 cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag
     6901 cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa
     6961 gaacttgaga atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa
     7021 catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg
     7081 aatgaagaac tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat
     7141 ttcgaagatt tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca
     7201 aatgcaaaaa aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat
     7261 tatgagaaag aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat
     7321 ctctcagcca tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc
     7381 cgtatcagta aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt
     7441 gagaaagtcg gctataccga actgggttat ttctccagag tttttaaaga agagacagga
     7501 atgacacctt ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc
     7561 atgaactctt tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg
     7621 tcatttcttt gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat
     7681 cgaatgatgt ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg
     7741 agaatacatt agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc
     7801 cagcccaaca tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc
     7861 tttcaaaaat ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa
     7921 cttttgtcgg aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga
     7981 ttgctcagag agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat
     8041 tgactcttcg cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat
     8101 tgaatcacgt ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt
     8161 ttcaatctgt agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga
     8221 tcatcgcatc aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa
     8281 cagtaggtga aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc
     8341 aggattgtac attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga
     8401 tttttccgat tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta
     8461 tgtatttgga tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag
     8521 aggaagatgg cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag
     8581 ggaatacgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag
     8641 acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt
     8701 ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag
     8761 cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag
     8821 aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta
     8881 tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat
     8941 ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca
     9001 ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg
     9061 aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt
     9121 tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat
     9181 atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc
     9241 ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt
     9301 attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg
     9361 tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga
     9421 gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt
     9481 ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc
     9541 caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga
     9601 cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa
     9661 aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat
     9721 gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat
     9781 acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt
     9841 ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg
     9901 ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga
     9961 cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct
    10021 ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga
    10081 tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga
    10141 atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt
    10201 atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc
    10261 ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc
    10321 taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc
    10381 ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac
    10441 aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga
    10501 ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg
    10561 gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa
    10621 ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat
    10681 gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg
    10741 aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca
    10801 gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg
    10861 gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt
    10921 cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga
    10981 tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca
    11041 gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta
    11101 aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct
    11161 ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt
    11221 ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg
    11281 gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt
    11341 ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt
    11401 ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt
    11461 tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg
    11521 tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc
    11581 gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag
    11641 tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt
    11701 ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct
    11761 tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac
    11821 tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc
    11881 caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta
    11941 atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat
    12001 aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac
    12061 tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag
    12121 aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca
    12181 catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt
    12241 ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga
    12301 tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct
    12361 gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga
    12421 tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt
    12481 ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta
    12541 tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt
    12601 cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat
    12661 tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat
    12721 gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac
    12781 tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat
    12841 aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg
    12901 tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt
    12961 ttgactgact atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg
    13021 ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg
    13081 cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca
    13141 acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca
    13201 ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct
    13261 ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg
    13321 ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg
    13381 cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc
    13441 acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag
    13501 gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg
    13561 tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta
    13621 tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca
    13681 acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca
    13741 ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt
    13801 tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca
    13861 gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa
    13921 tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact
    13981 tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg
    14041 acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt
    14101 tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg
    14161 atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct
    14221 ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt
    14281 gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat
    14341 attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg
    14401 atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc
    14461 tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc
    14521 aatagctact ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg
    14581 gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc
    14641 cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag
    14701 atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc
    14761 tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac
    14821 ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt
    14881 gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt
    14941 cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc
    15001 acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc
    15061 taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac
    15121 gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata
    15181 tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg
    15241 aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt
    15301 atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg
    15361 agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag
    15421 taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg
    15481 atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca
    15541 agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt
    15601 ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga
    15661 atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca
    15721 ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt
    15781 ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata
    15841 tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc
    15901 attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg
    15961 aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg
    16021 gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca
    16081 gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg
    16141 ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct
    16201 actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca
    16261 atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga
    16321 tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt
    16381 tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt
    16441 ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct
    16501 ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta
    16561 cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag
    16621 atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa
    16681 acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta
    16741 atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta
    16801 agaattgtac ctagtataga cataaaaaaa tcgactgtca atcattttta tgaaactttt
    16861 tttaattttc tgatagttaa ctttgttttt cagaaaaata acaattggtt ttattgaaga
    16921 aaatagtcat gaacaaaagt atctgagaac ttttttactc tcttgttcat gacatataga
    16981 ataaagcagc tttgtcttgt aaactccgtt tttcaaataa taatctaaag attgctactg
    17041 aataataaag ccgatttcat ggtcctcttc gctgctgatt cggtaaggtg actcaacatc
    17101 cgctccccag ctattgatac ccccgactcc tcttactttt gcaaaaatcg taagaaccgt
    17161 tcgtctttct ggcggtagtt cttctatatg tgttgcctgt tccagttctt ctgctttgaa
    17221 tggtaaagcg gaaaaagcaa atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc
    17281 ttgatcttta tccgtattat ccaacgtttg atttcttgtt agttctaccc atttcgtttc
    17341 catatgcatg ccgcattctt ggggaacaag gtatggggaa acggggagtc ctgccacttt
    17401 ataaatacct tcgacaccac cagccatccg atccggatat gtttcccctg ataggccttc
    17461 atactcaaaa aattttgctt tcgttggcat aatgaacctt aaacccaatg cgggtaattc
    17521 cggtaatcct ttttgtccat aataatgcat attgacaagc attttaccct ctccagcgat
    17581 ttggtaggaa attgtcactt gggtttttgg tactgtgttg atttccagtg taaaaatgat
    17641 ttcaaccgtt tcagcgtatt cattacttga atattgatta tttactggag caatcggcaa
    17701 atcgatcttt ttattgtcca ccagtacttc tatttctttg acttttggat acaatccggc
    17761 tcctaaccac ataccggatt taaatccaaa tccgctacct ctgtcattat cggtcgttgc
    17821 tcgccaaaaa actggaagtg tttcccggta tagccattct tttccgtttt tattgagcga
    17881 ttccagtccg cctcttgtat aagaaaaaat ataatgcgta ttccccgatt tgacacctaa
    17941 tgtacagtcg ccgaagacga ttgcaacttt ttccgtatcc atatgacttt tatttactgt
    18001 atttttcata ataatatctt acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa
    18061 cgatcccatt ccctgagaat tcatagtcac ttggtttatc gtcaaagtca ccgccatagc
    18121 gcatcacttc tctgcctgtg attggatcag tcacttggat cgcctgatct ttaaaatccc
    18181 atatatagcc tccttggtac atttcataac gatcaagcaa cgtcatgtaa gaatccattc
    18241 cacctaacga attccccatg tcatgcatat attcacataa gatgaacggt ttttccgctt
    18301 tattttcaag gtagtcaatg atctcttctg gtggcgcata catgcagctt tttacatctg
    18361 aaatcgaatc ttcgaatgct ggatttggaa aattcccttc ataatggaca agtcgatcgg
    18421 ggtctacttt tttgaagtaa tcgttcattt gtcgaatgtt ttcttccgca taagattcat
    18481 ttccaagaga ccaaaaaaga atacttggat ggttttttaa tgtttcaaaa ttcgtttttg
    18541 ctcgatcaag aacagccgcc tgccactgag gtactgcacc tggtacgttg taagaaggct
    18601 caaatgcgcc cattttttgc catgagccat gggattcgag gttcgtttcc gccatcacat
    18661 ggattcctgc ttggtcgcat aaataatacc aagtgatctg gttggggtaa tgagatgtcc
    18721 gtacgccgtt gatctttgca ttttttatga tagccaaatc tttttccata tcttctatag
    18781 tgatggcgcg tccttttttc ggatgccact catgtcgatt taccccgttg ataatcatcc
    18841 gttttccatt gaagtagatg attttatctt tgatttcgat tttacgaaaa ccgaaggaat
    18901 agtcaatata ctccgttact cttttttctt cgtctatcgt ctccaattca agcttatata
    18961 aattgggctg aaaatgcccc cacagtgcca cattttcaaa tccatcgatg cgaatcgtca
    19021 tttgctcttt taacggatat tgctgttgat agatttcctt cccatcagga tctagtatcg
    19081 ctacattcag ctgactgttt ttggctactt gctcaaaaga caaatctact tcaagtttgc
    19141 cattttgaca tttgtcatct aagcgaggtt tgatccaaag atcttctaaa tggttttgtc
    19201 ctcgagcaac taagaagaca tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt
    19261 ccaagaatgc agctgtactt cttttgtaaa cctccacagc tagaatattt tcttcttcca
    19321 aataaggtgt taaatcaaat tcagacggag tgaacgaatc ttcggcatac ccgataaaat
    19381 gaccattcaa ccaaataaac attgcttgtt ctacaccagc aaaatgaatc gagatttgtt
    19441 ttccctttag accttcttct aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt
    19501 tagcagctga aaagatccct tccgcctctt tctcagggct tgtcgtgaaa gggggacggc
    19561 gaaatacatg tccctcccac ggatacatcg tattgatgta atggatcttg tcataatttg
    19621 ctaattcaat atgactagga acagtgattt catcaaaaga acaggcatca aaatcttttt
    19681 tataaaatgt ttttggtcgt accgaaggat ttggcgaata acaaaaacgc catgatccat
    19741 ttaaagactg tttaagggag gatgtggttt ctacttcttc ccttgttgca taacaaacat
    19801 gatcgctatg tgcatttgat tggttcactc taaaaacttg tggatcatct aaccatttta
    19861 tatttgcttt catacttttt tccttctctc tcataacttt ttgaaatgaa gcagttctgt
    19921 ttggaaatca ccttgaaaat cggggaagta aagtccaact gtcatcaatt cggcccccga
    19981 aaatatccgc ttcgaggtct gttcctgata aagtgcctca tcatctaacc ccatcagata
    20041 tacttcatgg aacgctggtt gagctgaagc tagtatcctc cctaaaaaca caatagcttc
    20101 atcttgttta ggagacacaa acatccatgc ggcaatattt tcttcaaaag gactcgccaa
    20161 acgataataa tggccatact ggatcaccgg acgaatcgtt tgataggata taatttggtc
    20221 ttttacttgc ttttcttctt caggagacag ctttgtcaaa tccagttcat aaccaaaaac
    20281 agcgctcata gctgtttctc ctcttgtttt caacgaagta gatctgtcgg tctgatgatt
    20341 tggtacagct gatacatgag ctgtgatgga cgaaatcgga taagcaaggc ttgttccata
    20401 ttggattttc atacgctcca ctgcatccgt gttatcactt gtccatgact gcggcatata
    20461 ataaataaac ccggcatcaa accgtccacc tccaccagaa cagccttccc ataagatttc
    20521 cggataatca gttgtcaaat gttcaagtag ttcataaagc ccgagcatat aacgatgcgc
    20581 tacttctcct tgtctctgtg ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt
    20641 catatcccat ttgatgtagt caagaggaat cgtatctaaa atctttctca tttgtttctc
    20701 aatcgtctgc cgaacatcta accttgtaaa atccagtaca tgttgtgatc tggaagcaga
    20761 aggcattcta cctggctcct gcataagaaa atcaggatgt gttcgataca gttcagaatc
    20821 aaccgagatc atttcaggtt caaaccataa accaaatttc aatcctttgc tatgagcata
    20881 atctgctatt tcacgtaatc catttgtcaa cttgccttca tactcgaacc agtctcctaa
    20941 tgaagaattg tccgcatctc tttttccaaa ccagccatca tctaatacga acatttcgat
    21001 gcctagctcc tttgcttcat caatgatcgc cttgattttt tcactattga agtcaaagta
    21061 agtggcttcc cagttattga ccagaatcgg ccgttcagca aattgatgac gacctcttgc
    21121 gattctttca cgtagcaact catggtggat ctgactcatt ttgttcaatc cctgacttga
    21181 gtaagaaagg atcatttcag gtgtttgaaa agactggcct gcattcaatt cccacgaaaa
    21241 acgatggctg ttgatcccac cgacgactct caattgattg atctgatctt tttcaagttc
    21301 gaaagaatgg ttgccggaat aaacaaattg aagtccgatc gcttctccag tattttctgt
    21361 cgtatgatga tgaactaaag caataaagtt attcatatgg tgactgctcg ttcctcgacg
    21421 gctttcaaat acttttgttc cagataaaac agattgccgt gaaatctgtc tttcattgac
    21481 atgtgctcct ggcaaagcaa tgacttcatc aaatcgccta gtatgggcaa agtctagctg
    21541 gaaactagcc gctttttcta gtttgattgt ttctccggtt tcatttctta tctttacaga
    21601 tcgtgtgatc actgctctat cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt
    21661 ttctctatcc ttaagtgaaa tcaccaacgt ttgtgcctct gacttgtcca tcacataagt
    21721 ttgaggcaag ccttcgatct ctggcttccc atctgtaata taatggctgt catatctcag
    21781 atccagtaag tctgatccat tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat
    21841 gctgccatga gaagagtatt cttgcggtag tgtatctttc gacagagacc ggtcaggatt
    21901 taagggaaca tttcctgaaa atccacgatc tcttcgggga taatttttat tgtctttgta
    21961 ctgcttgact ggtttgccaa aataaatatg cgccaataca ttgctttctt ctatctttat
    22021 cagataagaa agaaatgtat ttcgtaaatg gaagactcgg ctcttttcat caaataagat
    22081 gaaattttta tctgtttttc tatgcgtaat atccatcatt tcactcccaa caaaacggaa
    22141 accgtttact tttttttata attcctgtat actgtttact aaacaatttt gtcaataaaa
    22201 attttttatt tttttgagga gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag
    22261 gcaaatgtct ctcctgctac agtttcccgt gtgttgaata acgatcagac gatccatgtg
    22321 gctcagacta ctcggcaacg gatagtagag gcagctgctg cgttggatta tgacatcagt
    22381 actcgtaaat atatcaaaaa gagaatgcct tcgattggtg tgatctctac gatcggcaaa
    22441 gcgagcgaag aacaggatat ctattacaaa catttgcgag tagggatgga agaagaagct
    22501 cgacgtctgc atttagggat gaatcgaatc tataatctat ctgataatcc taaacaatgg
    22561 gaagatctag atcagttagg tgcggtgatc gttgttggga ctgttacgaa agaatcgatt
    22621 accgccttgt taaaacaaaa catgcaaata gtcgtcgtgg ataaccctga catccagcaa
    22681 gaggtcgata tggtgtatgc agatcttgaa cggatgacta aaaccgtact ggaattgttc
    22741 ttagaaaatg gtcataaaag aatagcctat atcgggggat ataaagtcga tatggatgaa
    22801 ttcggtggga agcagatttc tataaatgaa aagaggatgc gtgcctatca gcattttatg
    22861 atcgatcatc acttagaaaa atatatgtct tatcatctag gtggctggac agaaaaacaa
    22921 ggtgaggtac ttgcgactaa tctattagaa aatgaatccc ctcttcctac tgccattctt
    22981 gtcgggagcg atccaatggc aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa
    23041 attggagaag agattgttgt agcaagcttt gataatatcg agacaatcgc aaacctctcc
    23101 cctggcttga ccagtgttca aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg
    23161 gcattggagc gaattgacgg tatacgggac gaaccaatca ttttgactta tcctgcacga
    23221 ttgattgtta gggaaagttt tatcccaaga acaaaaaggt attgaaagag aggttgtgaa
    23281 aagccgcttc gtttcttcat actcaaaacg aaacggcttt ttcacaacct cttacttata
    23341 cttattcaca aaatccaatg tctgctggta acaattttgc gattctttta gcgtgtaatc
    23401 aaattggtat tcatgggtaa tctctttttt tgtatctgta taaaacaagg attgcacggg
    23461 tacctttaat tctgtcaacc gattttctaa tgcttgacct tgctcctgga atgagtatgc
    23521 atttccgtca gttatatacg ttggcggaaa ttccttagaa acatggtcag ccacactagc
    23581 ttctttcaat tgctcactcg ttttccaatc ttttgttcca atcaacgacc acgcaacggt
    23641 tttgacgaaa aacttcataa atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc
    23701 aacaggtccg caataagaaa tggttccttt cagactatct tttggaagta gctgtttgat
    23761 tttcatttgt ttagcatacg catcattcgt ttgtatagct gcatattgca atgcgatttg
    23821 tgaacctgca ctatctccac caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt
    23881 ttcttcagct aataaatctt tgatcagttc attcacttga agaacttgat tagggtattc
    23941 tgaatcaggc gctaattcat aattcatcgc aaccactgcg atgttggcgt ctgaaactaa
    24001 ttttgtcgca aattctttga cacccgattt atctccgccg acgaatcctc caccatgtac
    24061 ccaaaataga acaggaacag gccccttgct gttttttgga tagtagatgt cataagtatt
    24121 ctctttatac ttggactcat acgtcaaatc tgtttttttc gccacgtttt tttctgcttg
    24181 atcgaacgtt tttttatcag tgatttgaac agtatttgaa aacatatggt tgatcaagaa
    24241 tgctccaggt ctgggggaaa gctggaaggc aagaaagatt gcaaacgcga tcccaagaat
    24301 gattccgaag aaccatccga taaatttaag aattttcttt ttcataaggc tccctcagta
    24361 gttagtatta agcattctga ttataccata gtgttatttc ctatcaagct ctcctagatg
    24421 atttaatgat tttccccaaa cttcttctgc aatgcttttg acaaaagaaa ttttttccat
    24481 tgttcttcaa acaaaacatt ttcttcttct gttgaagcaa atccacattg aggacttaag
    24541 cacaaacgat ccaaaggcag atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg
    24601 tgctcttcca tttcgccatt tttcgttaca gtcaaaatct aagacaacta atttatcttc
    24661 agagactttc tctaaaagaa caaaactccc agcacgttca ctatcgtatt ctaaataata
    24721 ggcacggaca ttttcttgag taaaataatg ggtctgctac gtcgtcatcc cccctgatgc
    24781 cgccaaaatc gaataataat ttccgcgtca aacatgtata tttagtgttg aatcatttga
    24841 gaatatgaaa ataaaaatct ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat
    24901 ctgagacaga atgacgttca ttctgaccca gatttgattt tgcacaactc aacagtcctt
    24961 attggcaaat cgccaaaata atgtagacta actaaaaaga ctaagaaaca ataacaaaag
    25021 ttaacacttc ttagtctttc tttagcacgt ctatttatta aagattttag ggccttttct
    25081 catgttttta cgatcattct taggtgtttc catttgtttg gatcggcctc caccttgttt
    25141 ctttttgatc aattctttca ttttttcttt ttggtccatt tttattctcc tttaacgaat
    25201 gaagcgacta attcgatatg atgggtttgc gggaaaagat ctacaggctg aactttcttc
    25261 acggcataac ctgcttctgc aaataactta gcatctcttg caaaagttgc cggattacat
    25321 gagatataga cgattcgttc tggtgacatt tcgattgctg attcaataaa atgatgatct
    25381 aatccttttc gtggagggtc aactacgatc acagatggtt tgatcccttt ttcttgccat
    25441 tttttcataa ctttttctgc ttttccgact tcataatgaa cattttcgat tttattattt
    25501 aaagcattga actgtgcatc ttcaattgct tctggaataa cttccatccc atacacctta
    25561 tccactctgt ctgctaaaga caagccaatc gtacctattc ctgaataagc gtcgatcaca
    25621 atgtcttttt tatctaactc ggcaaactcg atggctgttt ggtacagata ctctgcttgt
    25681 tgagtattta cttgataaaa tgactttgct gagattcgat aggtttttcc taatagctga
    25741 tcttcgatat agtctctgcc atataaaact ttttctttgt ctcctaaaat cacatttgtt
    25801 ttttcttcat tgatattttg gatgaccgaa accacttctg gcaattcttc atgaatagct
    25861 tcagcaattt ccttggattt aaagaatttt tctttacgag taactaaaac gaccatcatt
    25921 tcatgggaat aatgaccacg acggataatg atgtgacgaa gctgtccttc atttttttct
    25981 tcattatatc ctcgtacttg gaaccgttgc aagatatctc tgactacttt gattgcttga
    26041 tcgatgacag gatcttggat ataaaaatct tcgataggga ctagatcatg gctattcttt
    26101 ttgtaaaaac cggtggctaa ctgtccatcg atacggcgaa cagggatctg tgctttatta
    26161 cggtagccga atggttctgc cattccgacc gttggaagta caggaacttc tggcatctta
    26221 gcaatcttcg ccattacatg ctctacttgt ttttgtttga agacaagctg ttgttcatac
    26281 ttcaaatggg ctaaaggtgc gatacctatg cgaagtaatg cattgttttc tacaggctga
    26341 cgatcaggtg attgtgtaat aaatgtttcg acttttccat atccaaactt attgcctgct
    26401 tttactacta gtatttctac ttcttctcca gggagtgcat tttcgataaa taatggataa
    26461 ccatcgattt ttgctacccc cattccttca taactcaagt cgacgatttc gactgtatat
    26521 cgttcgtttt tctttactat ttgctgttct gtcatatttt gctcctttaa gcctattttt
    26581 caatcctact gaactctact ataaatcagc taattacaca actaaaaccg gcactgacct
    26641 tactacgttc agcaccggct tttgattaat tcttttgatt aggtgtttcg tctgctattt
    26701 ttccgtcacc gtcgatatct tcatccgtta aacgttcgac ttcttttacg aattctttgc
    26761 tgacaacctc taattcttct tcatctgtac ctgtgattgc ttcatcagga atctgatcgg
    26821 tattggcaaa gaactcaatg tgttgatgaa gattttggaa atgcatcggt gcttccccgc
    26881 cgtattcacc atctaagttg atcattactg gtcgattgat atcatcttct gtttcaacga
    26941 acaaactgct tgttttggta tagattattc ttggatcatc gacatgtcga ccgccattca
    27001 gcatgagtgc tgcaatatgc aagatctcaa agatatttgc tgtcttcaca atgattaaag
    27061 aaaacttccc atcatccaat ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt
    27121 tcgtcagccc taggaaaaac atcgaagcat ttccgatata ttccccttca tcatacttca
    27181 agcgcatttt gattggcttt actcgaggga gcatctctgc tcctttcgca agataagcaa
    27241 gataaccaaa aatactcttt agttctgatg gaacttcata agtcaattct gtcaaatagc
    27301 cacctgcagc gatattgatg aagtaactgt tatcagaaag gccgatgtcc atttttacgg
    27361 tttggtgttt ttttatcact tctgctgctg ctttaatatt atctctagga atttttaatg
    27421 ctcgagcata gtcgttcgtt gtaccagctg ggatgatggc cattttagga cggtgttcaa
    27481 gcggagcgat tccattaacc acttcgttga ttgtgccatc gccaccagct gccactacta
    27541 gatcaaaacc tagttctgcg actctacatg cttcattctt ggctgaatct ggttctggtg
    27601 tagttgcata ggcgctggct tcaaatcctg cttcttctaa tgcctgtagg atatccgcca
    27661 aatttttttt caataattct tttcccgagg tggggttata aattacgcgt gcttttttca
    27721 ttttcagcct ctatcgtttc gctaattcat ctttaagtag cttattcacg acaccaggat
    27781 ttgcttgtcc tttagttgct ttcatgattt gaccgactag gaagccaacc gcacgatctt
    27841 ttccattttt gaaatcatcg acagattgtt ggttgttatc caaaatttca ttaatgattg
    27901 gcagtagttt gttcggatca gataattgca cccaaccatt tttttcaact acttcttggg
    27961 cttctccgcc atttgtgatc aattcacgga agactttttt ggcgattttt gagctgatcg
    28021 ttccatcggc gatcaaacga atcattcctg ctaagttctc aggtgtcaat tttgtctcag
    28081 ctaattccag tttctcactg ttcaagtaag cagaaacttc acccatcaac cagttagatg
    28141 cttgcttggc atctgcacca ttttccaaaa cagcttcaaa aaaatcagac atttcttttg
    28201 taagtgtcaa taccattgcg tcatactctg gaagtccaaa ctcacggata tagcgttcgc
    28261 gacgagaagc tggcatctca ggcaaactag cttttacttt ttgaatccat tcgtcatcga
    28321 tcacgaattt cgggatatca ggctctggga agtaacgata atcactagac ccttctttga
    28381 cacgcatcaa aactgtttta ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt
    28441 ctcctcctga aagaagaact tttgcttgac gtttttcttc aaaagctaaa ccttttttca
    28501 cgaaagataa agagttcaag tttttcaatt ctgctttcgt accgaactct tcttgtccat
    28561 aagggcgaag agaaatattg gcatcacagc gcatagagcc ttcttccatt ttcacatcgc
    28621 tgacttctgt aaattggata attgatcgca atgcttctag ataagcgtat gcttcttctg
    28681 gtgaacgcat atcagcttca gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat
    28741 caacgtaaga ataaccgcca ataccatgca tgttttttcc agcatcttct tctaaatgga
    28801 cacgttcgat ccggattttt ttctttttac cttctacttc gatttcgatc catccgtcat
    28861 gaccgattgg ttgatcaaat tgggaaattt gatatgcttt tggattatct gggtaaaagt
    28921 aatttttccg gtcaaaatga gtatctttag agatttcaca gtttaatgcc aaagctgctt
    28981 tcatcccgaa ttccagcgca gctttattca ttactggcag cacgcctgga taaccccagt
    29041 cgatcacgtt tgtattgctg tttggttctg caccaaaatg agctggcgct ggagagaaaa
    29101 tttttgagtt ggtttttaac tcgacgtgga cttctagtcc aatgactgtt tcaaaattca
    29161 ttcgtctttc cctcctaaaa tagctggttg ttgtttatgg aattctgttg cttgttcaaa
    29221 cgcatatgcc gcttgataca tcgttttttc atcaaaataa ttaccgatga tctgcaatcc
    29281 aactggtaat ccttctgata aacctgccgg tacagacata cctggaagtc cagccaagtt
    29341 gacagggatc gttaagatgt cactcatgta cattgtgatt ggatcgttaa tgttctcacc
    29401 gattccgaat gcaactgttg gtgaagatgg cccgatgatc aagtcataat ctgcaaagac
    29461 tttttcaaaa tctcgtttga tcaatgtacg gacttgtcct gcttttttga agtaagcgtc
    29521 ataataaccc gcgcttaagg agaatgtacc taacatgata cggcgtttta cttcttcacc
    29581 gaatccttct gaacgtgagt tgacatatac atcatctaat gatttcacat tttcagagcg
    29641 ataaccgtag cggataccat cgaatcgttg caaattggaa cttgcttctg atgaggcaat
    29701 gatataataa acagccacac cgtattttga atgaggcaag ctgacttctt ccacaacagc
    29761 acctaatgaa cggaatgttt ctgctgcttt caatacagct tcttttacgc caggggccac
    29821 accttcgcct aagtattctt taggtaaagc aatcttcatt cctttgatgt cgcctgtcag
    29881 tccctctgca aaatcaggaa ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc
    29941 gctgatagct gtcaatgcta atgcattatc tttcaccgta cgggtcattg gtccgatttg
    30001 gtcaagagat gaagcaaagg caatcaaacc aaaacgtgag acacggccat aagtaggttt
    30061 catccctacg atgccgttaa aagctgctgg ttggcggata cttcccccag tatcactacc
    30121 taaagaaact ggaatttgtc cagctgcaac tgctgctgca gaaccaccag aagagccacc
    30181 aggtactttt gttgggtccc aagcattttt tgttttcttg aaataagaag tttctgtact
    30241 tccacccata gcaaattcat ccatgtttag cttaccaaca gggatcatat ctgcttgata
    30301 gattttatcc atgaccgttg catcataaat tggttcaaaa ttgtaaagga tcttagatgc
    30361 agcggttgtc aagatatctt ttgttacgat attatccttg ataccaattg gaataccagc
    30421 aaggacgttg ctttcatcaa tccctttttc atctaaagct tttgcctgtt ccaatgcttt
    30481 ttcttcgttc aatgtaatga atgcctcgac ctcaggctct gtttctttga ttcgttcaaa
    30541 tgttgttttt gtcaaatctt gtacagtgat ttctttcgat acaagcatat gatgcagttc
    30601 ttctagagaa cgattgtgta attctgtcat tatgcgccag cctccccatt atcgatgatt
    30661 gcgggtactt tgataaagcc gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg
    30721 ttccatccag cttgcgcccg atcttgtcgc atcacgttta ctgtttcaac cacattcgac
    30781 gtaaatggaa caccttccgt atctacttct tccagcattt caaccatatc gatgatttta
    30841 cctaattggt cggtaaaact tgcaagttct tcatctgcga acgcaagttt tgctaatttg
    30901 gcaacgtgtt tcacttgttc ttcactaatc gccatactac cccttctttc taaacgagtt
    30961 aaaaactttg ctctctattt taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt
    31021 tgtctgaaaa aactcatttt tcattcttct actgagcgac ttttagccaa tgcatctgcc
    31081 atttgatctt ctgtccaaac ttcgattcct aattcttgtg ctttggtcag cttgctgcca
    31141 gcatcttctc ctgctaccac gatatccgtt tttttcgata cgctaccagt cactttcccg
    31201 ccaagatttt cgatcgtctc ttttgcttct tcacgagtaa atcgtgttag tttaccagta
    31261 aggactactg ttttttcttt aaaaggagac tcgacttctt gcaattgggc atttcgaagc
    31321 cctttgtatt cgaaattgac gcctgcttgt tttaattcat tcatcaattc atggacttct
    31381 tcgttactga aataagtgac tacactgtcg gcaatgatat caccgatcgt atcaagcgca
    31441 ataatcgact catagtcact tttactcaat gtttctagat caccgaaatg ttctgctaaa
    31501 atttttgctg ctttcgctcc gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc
    31561 gaattatctt tgctgttgtc gattgctgtc aaaatattat ttgctgattt ttcttttatt
    31621 ttgtccaacg tcaataactc ttcttctgtc aatttgtaaa gatcagcaac atctgctact
    31681 agttttttat catacatctg ttctagtacg cgcggcccca acccatcgat attcatcgca
    31741 tttcttgaaa caaagtggtt caaaccttct ttcatttgag cagggcattt aggattgatg
    31801 caccgtaagg ctacttcttc atccaaatgg acaagttcac ttccacaaac tggacaatgt
    31861 gtaggcaatt ggtattcttc gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact
    31921 tctgggatga tgtctcccgc tttataaatc agaactgtat ctttcaagcg gatatccttc
    31981 aacttaatat aatcaccgtt atgcaggctg gcacggctta ctgtcgttcc tgctactcgg
    32041 actggtgtca tgatagctgt tggcgttact acccctgttc gaccgactgt ccattcgatg
    32101 ttttcgatta gcgtttcaac ttcttctggc ggaaacttgt aagctgctgc ccatctcggt
    32161 gctttgatag taaaaccaag ttgatcttgt agcgagaatt cattcacttt gatgacgatt
    32221 ccatcgattt catagggcaa atccacacgt ttgtcatgat attcttcaat gtatgaccac
    32281 acttcatcaa tcgaatgaca tagacgtttt tcccgattcg tgtggaatcc aattttttct
    32341 agctcttcta gcgcatcata ctgggttttt gcttgcatag gaccgaaatc agctactgtg
    32401 tataaaaaag tgtccagatt tcttttcgct gtgattttag aatctaactg acgcaaagaa
    32461 ccagctgcgg cgttccgagg attcgcaaag atatcttttc cctctgcttc tctgtcttgg
    32521 ttcaattgga caaatgaccg ttttggcatg aaacattcgc ctcttacttc gatgttcatt
    32581 ggttctttta atttgattgg gaccgaccgg acagttttta ggttttcagt gatgttttct
    32641 cctacagttc catccccgcg agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa
    32701 gaaacggaaa gtccatctat ttttagttca cagcaatagt caactacacg tcccactgct
    32761 ttttgtacac gttgatcaaa agctatcagc tcttctttgc tgaaaacgtc gttcaaactg
    32821 taaagcggaa tatcatgagt gactttttca aaaccttcta atacttttcc gcctactcgt
    32881 tgagtgggag aatcagacgt gatcaaatca ggatattgtt cctcgattgc tactaattct
    32941 gcgtattctt tatcataaac atagtcttcc actgttggtt tatcttctac gtagtattcg
    33001 cgagaccatt ggttcagtct tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc
    33061 gtcatttcat gttccatcat ttaccctcca ctactaagct ttttcaattg gcgcaaaggc
    33121 agccaaaaga cgttttactc cttgacttgg gaaagccaca tctagttcta gatccttcga
    33181 cgtcccgctt actcggacga ctgttcctag accccatttt ttgtgtttga ctttatctcc
    33241 aggtttccag ctgttatttt cccctccagt agcactttta gagggaacaa ccgctgtctc
    33301 tggctgttta tagctatatg ctgttttttt agcgaataca ggcgtttttt taggcgtagg
    33361 acgcatgcct tcgatttcca gtaactcctg atcgatttct tctacaaaac gagaaggtcg
    33421 gttatattgc gtccgtccat ataatgttct ggagaaagca tttgttaaat acaacgcttc
    33481 ttctgctcgc gtgatcccca catatgcgag acggcgttct tcctccagtt cgctttcttc
    33541 catcaatgct cgagataaag gaaaaacccc ctcttctaga ccaatcagga atacaactgg
    33601 aaattctaat cctttggctg catgcaaagt catcaatgtc acttgggaag catcttcttc
    33661 taaattgtca atgtcggaaa caagtgccag atcatttaag aagaccgtta gtttttcttc
    33721 tggtgcatca gcgtcttctt cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac
    33781 cgttaaaaat tcctccaagt tttcgatgcg tgcttgtgct tctaatgtat tttggatttt
    33841 taagtcttct agatatccgc tacgatccaa aacctctttc gtcaattctg tcactgtcaa
    33901 gtatgggatc atctgagtga cttcttggat catctctcca aatgcaccta attgctgtcc
    33961 tgcttttcca ccgatatttg ccagatccac attttgcgct gcttcaagca gtggccattc
    34021 atgaagagaa gcaaaactgc gtagtttttc gatagatcct ggtccgatgc ctcgtttggg
    34081 cgaattaacg atacgctcaa aactgattga atcttgagga tttaccagca cattcaaata
    34141 cgctaaaata tctttgattt ctttacgatc gtagaacttg tgccctccaa ccattttata
    34201 aggaatattt gctttcaaca gagtttcttc cattacacga gattgtgcgt tcgtccggta
    34261 caagatggca aagtcgccgt aattccgatg attgcttcga atctcttctt gcatccggtc
    34321 tacgataaaa cgggtttcgt ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc
    34381 ttctttgttt tctgtccaca agttttttgg ttttcgattg ctattgtttt caatgacttg
    34441 gtttgctgcg ctaaggatgt ttttcgtgga gcgatagttt tgttctagca aaattactgc
    34501 agcatctgga taatcttttt caaaatccaa gatgttctgc atatctgcac cgcgccagcc
    34561 gtaaatactt tgatctgcat ctcccacgac gcacaggttc ctaaatctac ctgccaataa
    34621 attgactagc gtatactgtg cgtgatttgt atcttggtac tcatctacat gaatataatg
    34681 gaatttgttt tgataatacg tgagtgaatc aggatgttct tcaaataatc gaatcgtatt
    34741 catgatcagg tcgtcaaagt ccatacattg attatattga agctcttttt ggtaagcagc
    34801 ataacatttt gccgcaatct cttcgtaata actgccttgc atttttgcaa aatcctgagg
    34861 ggtctgcagg ctgttttttg cttgagagat tgttcctaat atacttcgag gatcatattt
    34921 tttcggatca atgttcagtt cttttagtat ccgtttcatc aaggtcagct gttcagaaga
    34981 atcgatgatt gtaaagttcc ggttataccc gataaaatca acatctcttc ttaaaatacg
    35041 cacacacatc gagtgaaaag tcgataccca gacatcttcg cctccagatg cgagaatcgc
    35101 attgacacgt tctttcattt cttttgcagc cttattggta aaagtaatcg ctaggatgtt
    35161 ccaaggattg acttcttttt cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt
    35221 ctttccactt cctgcacctg ccattagcag taatggtccg tcagtgtgaa gaacagcttc
    35281 tttctgacgg gggttcatcc cgtttaataa ttcagctttg gatgacatta acaacatcct
    35341 ttcttttatc aatcttgtta attatagcat tttcttggct ttcattaaac tagctagttc
    35401 aaaatcattt gtttcttttt atggattttt ccttgtcaat tcgttgattt cgcgctaaat
    35461 taaaagaaag cgaggcgttt gaatggaaaa aaaaggaaaa atcaagaaat tagataagac
    35521 tcagatatat tttattctaa aaggaatatt tgttggactg ctgacaggaa tcgtcgttag
    35581 cctgtttcga ttatctatcg aaaagctaag cgatttagtt cgaagtatct atgaaatgag
    35641 tcgggaacag ccgatttact taattggcct agctatttgc tgcatccttg cagcattttt
    35701 cgtgggttat ctggtcaaaa atgaaccgga tatcaaggga agcgggattc cacaagtagt
    35761 gggacagctt cgcggggaat tatcaatgaa ctggttctct gttttatgga aaaagttcat
    35821 tggcggtgta ctttcagttg gtgcgggcct ttttctaggt agagaaggac cttctataca
    35881 gcttggcgca tccattggac aaggagctgg acaattgttt cggagtccat cttcagaaga
    35941 aaagatttta atttctagcg gtgcaagtag cggacttgca gcagcattca atgctcctat
    36001 cgcaggatta ctctttgttt tagaagaaat ccatcactcc ttttctccac ttgtttggct
    36061 gacatcattt gcttcagcaa tcactgctaa cttcgtctct ctttactttt tcggcttgag
    36121 acctgtttta tatctaggtg accttccttc tttgccgttg aaatattatg gttccttagt
    36181 gatcttagga gtacttctag gcatcttagg ttttatttat cagaatgtct tgctggcttt
    36241 gccaaaattt tacaaccgat tgcctttacc agcttatttt cacgggttcg ttcctttctt
    36301 gctgattttg ccaattggtt atttatggcc tagtactcta ggcggaggca atcaaatcgt
    36361 tttagctttt ggacatgctt ccttgccaat cattatgatt atcggtttgt ttgttcttcg
    36421 attcgttttt tccatgattt cttacggtgg gggtcttcca ggggggattt tccttccgat
    36481 attgacattg ggggctttaa tcggtagctt ttatggaaac attatggttg atttcgggat
    36541 ggatccaatc tacatcaagg attttgtcgt tattgccatg gctggttatt tcacagcgat
    36601 tggaaaagca ccgctgacag caattatttt agtgacagaa atggttggct ctcttaatca
    36661 tctgatgcct ttaggattgg ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg
    36721 taatccaatt tacgaatcat tattggaacg tctacttgga aatactcaga ccgcctcact
    36781 aaaaggtgca aggacaactt ttgattttcc agtaacggca gagagttctt tagacggatt
    36841 aatggtacga gacttcaatt ggcctaaaaa tatgctgcta atctctatac gcagaggaaa
    36901 tcaggaaatc ttgactcacg gtgatacgat catgaatgta ggagatattt tggttatctt
    36961 gactgatgaa gcaaacttgc caagaatcaa gcaggaaatc gaggctaaat cttctatcac
    37021 aaatttgaag caatcatttt aacgaacatg gaaaaactat tttacaacaa ctattcatct
    37081 acactacttg attctatatg attctataaa aaaagaagag gctgggaaaa cccagcct
//
LOCUS       DAFCHQ010000016        36900 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000016 DAFCHQ010000000
VERSION     DAFCHQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.016"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..168)
                     /locus_tag="LNC71_000924"
                     /pseudo
     CDS             complement(1..168)
                     /locus_tag="LNC71_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(422..2057)
                     /locus_tag="LNC71_000925"
                     /pseudo
     CDS             complement(422..2057)
                     /locus_tag="LNC71_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2380..2859)
                     /gene="rlmH"
                     /locus_tag="LNC71_000926"
     CDS             complement(2380..2859)
                     /gene="rlmH"
                     /locus_tag="LNC71_000926"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBK6466015.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3287..4576
                     /locus_tag="LNC71_000927"
     CDS             3287..4576
                     /locus_tag="LNC71_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBK6466016.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4615..5220)
                     /locus_tag="LNC71_000928"
     CDS             complement(4615..5220)
                     /locus_tag="LNC71_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBK6466017.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5425..5889)
                     /locus_tag="LNC71_000929"
     CDS             complement(5425..5889)
                     /locus_tag="LNC71_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6466018.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5911..6231)
                     /locus_tag="LNC71_000930"
     CDS             complement(5911..6231)
                     /locus_tag="LNC71_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK6466019.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6375..7454)
                     /gene="pepA"
                     /locus_tag="LNC71_000931"
     CDS             complement(6375..7454)
                     /gene="pepA"
                     /locus_tag="LNC71_000931"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBK6466020.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7654..8004
                     /locus_tag="LNC71_000932"
     CDS             7654..8004
                     /locus_tag="LNC71_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK6466021.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8227..9174
                     /locus_tag="LNC71_000933"
     CDS             8227..9174
                     /locus_tag="LNC71_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6466022.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9231..10649)
                     /gene="pepV"
                     /locus_tag="LNC71_000934"
     CDS             complement(9231..10649)
                     /gene="pepV"
                     /locus_tag="LNC71_000934"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBK6466023.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10798..11643
                     /locus_tag="LNC71_000935"
     CDS             10798..11643
                     /locus_tag="LNC71_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBK6466024.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11647..12087
                     /locus_tag="LNC71_000936"
     CDS             11647..12087
                     /locus_tag="LNC71_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBK6466025.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12137..12331
                     /locus_tag="LNC71_000937"
     CDS             12137..12331
                     /locus_tag="LNC71_000937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466026.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12467..13153
                     /locus_tag="LNC71_000938"
     CDS             12467..13153
                     /locus_tag="LNC71_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6466027.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13561..14250)
                     /locus_tag="LNC71_000939"
     CDS             complement(13561..14250)
                     /locus_tag="LNC71_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466028.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14225..16354)
                     /locus_tag="LNC71_000940"
     CDS             complement(14225..16354)
                     /locus_tag="LNC71_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBK6466029.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16402..17739)
                     /locus_tag="LNC71_000941"
     CDS             complement(16402..17739)
                     /locus_tag="LNC71_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBK6466030.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17991..18350)
                     /gene="rplT"
                     /locus_tag="LNC71_000942"
     CDS             complement(17991..18350)
                     /gene="rplT"
                     /locus_tag="LNC71_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBK6466031.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18403..18603)
                     /gene="rpmI"
                     /locus_tag="LNC71_000943"
     CDS             complement(18403..18603)
                     /gene="rpmI"
                     /locus_tag="LNC71_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBK6466032.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18675..19199)
                     /gene="infC"
                     /locus_tag="LNC71_000944"
     CDS             complement(18675..19199)
                     /gene="infC"
                     /locus_tag="LNC71_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBK6466033.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19243..19368)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19519..20151
                     /locus_tag="LNC71_000945"
     CDS             19519..20151
                     /locus_tag="LNC71_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK6466034.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20153..21058)
                     /locus_tag="LNC71_000946"
     CDS             complement(20153..21058)
                     /locus_tag="LNC71_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6466035.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21143..21802)
                     /locus_tag="LNC71_000947"
     CDS             complement(21143..21802)
                     /locus_tag="LNC71_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6466036.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22118..23062
                     /gene="mvk"
                     /locus_tag="LNC71_000948"
     CDS             22118..23062
                     /gene="mvk"
                     /locus_tag="LNC71_000948"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBK6466037.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23055..24032
                     /gene="mvaD"
                     /locus_tag="LNC71_000949"
     CDS             23055..24032
                     /gene="mvaD"
                     /locus_tag="LNC71_000949"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBK6466038.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24045..25130
                     /locus_tag="LNC71_000950"
     CDS             24045..25130
                     /locus_tag="LNC71_000950"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBK6466039.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25127..26191
                     /locus_tag="LNC71_000951"
     CDS             25127..26191
                     /locus_tag="LNC71_000951"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBK6466040.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26440..27243)
                     /locus_tag="LNC71_000952"
     CDS             complement(26440..27243)
                     /locus_tag="LNC71_000952"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBK6466041.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27263..28105)
                     /gene="kduI"
                     /locus_tag="LNC71_000953"
     CDS             complement(27263..28105)
                     /gene="kduI"
                     /locus_tag="LNC71_000953"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBK6466042.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28121..29128)
                     /locus_tag="LNC71_000954"
     CDS             complement(28121..29128)
                     /locus_tag="LNC71_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBK6466043.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29135..29779)
                     /locus_tag="LNC71_000955"
     CDS             complement(29135..29779)
                     /locus_tag="LNC71_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBK6466044.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29879..30637)
                     /locus_tag="LNC71_000956"
     CDS             complement(29879..30637)
                     /locus_tag="LNC71_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBK6466045.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30764..31915)
                     /gene="fucO"
                     /locus_tag="LNC71_000957"
     CDS             complement(30764..31915)
                     /gene="fucO"
                     /locus_tag="LNC71_000957"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBK6466046.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31997..32308)
                     /gene="rhaM"
                     /locus_tag="LNC71_000958"
     CDS             complement(31997..32308)
                     /gene="rhaM"
                     /locus_tag="LNC71_000958"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBK6466047.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32310..33155)
                     /gene="rhaD"
                     /locus_tag="LNC71_000959"
     CDS             complement(32310..33155)
                     /gene="rhaD"
                     /locus_tag="LNC71_000959"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBK6466048.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33169..34425)
                     /gene="rhaA"
                     /locus_tag="LNC71_000960"
     CDS             complement(33169..34425)
                     /gene="rhaA"
                     /locus_tag="LNC71_000960"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBK6466049.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34422..35882)
                     /gene="rhaB"
                     /locus_tag="LNC71_000961"
     CDS             complement(34422..35882)
                     /gene="rhaB"
                     /locus_tag="LNC71_000961"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBK6466050.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            36035..>36900
                     /locus_tag="LNC71_000962"
     CDS             36035..>36900
                     /locus_tag="LNC71_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBK6466051.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKF"
BASE COUNT        10732 a         7761 c         6752 g        11655 t
ORIGIN      
        1 catattagga attggatctt cacctggcat atattgcaga ttagaagcat gtaatttttt
       61 ctcagaaaca aaccactcat tggaaaagac atgaatcaaa tcatcactta cttgtgtaaa
      121 atagcgacgt ttaatgacaa agatatcttc attgctatct agtgtacctt catttctttg
      181 tgcttaaatt caaagataat tttctcaata aagatttttg attccagtaa gattccttta
      241 ctccttttac aacatagcgt tgtcctttgt tttgaaacat cttcttcccc tctttgccat
      301 taaaaatagc ccaattctct ataaagaaga ttaagcttca ttattataat ataaagttta
      361 aatttggttt attacttact ttttcttatt ctctagtaag ctaatattga aaaaaatgta
      421 atcagatcat tatttttata tgttgtaact tcctgataag aaaactaatt aaaagaaaga
      481 ggtaactaca taaactaatt aaaaaagcta acttcatagt tggaggataa atggaagaaa
      541 cttcaatgat tgtttctcct tgtgttcctt caatcgacac tagtcctatt atcaatctct
      601 cttttttgtc catttacttt tgcgactgtt ccccaataat caaatacagg aagcgttatt
      661 ttttgtcctt tagattcatg tactgtatag actattttcg aatccatcac ttgataaggc
      721 atagtatgcc attccttgcc aattcttact tcatgattct gcaaatgttc tttgtattgt
      781 aatgcttccg tgggtgcata gtcataaagt cctccgggaa ggagtgtcgt gtaacgaata
      841 agattttctt gattcattac caagtgatct ctctgagtga caactttatg ttcatagttt
      901 gcttttgttg cagcagatac agccaacagt aataaaacaa ttgccgttat attaactact
      961 tttctttgct tagctgatgt gctcttcatg aaaacgaacg ataaaatcaa cgagccaaac
     1021 agtacttgaa agcctaagat tcgccaagga aattgaatca tttgaatggg tgtattttgt
     1081 aataaagacc atggaaaaaa tttggtggat aagagccaag agactaccgc actaaggtaa
     1141 atcacttttt ctttgcgatt aaatccttta aaaaagaaga gtggacagat caaagaaata
     1201 ataccaatga ttcctaccgt aaaggttgtc aggtcattat tcatcgcacc aatcaaacta
     1261 tccgagacat ttaatgcttg gtgttgtaag gtcgttttat ctggctcatt aatcgattga
     1321 accatgattt gtcggaacat cggataccaa aagtatgctg ttaaaagaat cgtcattcct
     1381 actgcctttg acaactccac aatcaaccgg atgctttctt tttttcttgt ttcacttact
     1441 acaaagccgc ctaagactag taagccaacg gctattacac tcataaaagt ggaaagaaca
     1501 tgtgtataaa tgagtaagct catactgact gctaatggaa ttgcacatgg ctttttctca
     1561 taaatcagtg cgtaagcata atacataact atcggtataa ccgtaagtgc aatcgcttca
     1621 gcaatagcag aacgatgata aatatcaatc gtacggtacc cataaaatgt atataaacag
     1681 ctaaatataa aagccgcatc gattcttttt aaaaatttta gtccataatg aaacatcagc
     1741 atgatagtac aaacattcaa tagccaaaca tataagatat aactaacgat taaattatta
     1801 cttatccaat aaaaaatgac tgccggaaaa aaggtcaagt agggataaaa ataattcact
     1861 ccgctcccat aatgattaaa cgtcgtaaaa ttgattggac cagaaaaaat tgaagataac
     1921 cctttgactc tagcaaaatg gaaggcaata tcccccgatg tagaaatagt atgaacattt
     1981 ctaaaataat tgattgaata aagtccagca ataattgcta ggagaatgga caatattatt
     2041 tttttcttta cttgcattac ttaattatat actcctttca tggattgctt tatctagtat
     2101 aacttaaatt ttaaataaat cgttatttat ttttactctc acttaaaaaa gcactaaaat
     2161 tataagagag ggaggtaatg gtctgaacac caagaattgt tccaacatcg aattgcttaa
     2221 ctaatgagtt caaagcttaa ttatgtggct cttatattta ccgagcgtgg attgcgatct
     2281 ctttagcgaa acaaacatct ttcataaaaa aaacagccat atccgctttc ttggatatga
     2341 ctatttacta ctactttact atctttcatt cttcttgtct cacttatgat aaggcgcccc
     2401 cgcattgatc cggaaggcac ggtagatttg ctcgacaagg atcagacgca tcaattgatg
     2461 tggatacgtc atttttccaa aagagatctg agcattgctt cttttcatga cggcttcgct
     2521 gagtcctaaa gagccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg
     2581 gtcgatttgt ttagcaaaag attcacttgt aggatttttc ccttcaattg ctaaggcata
     2641 gacgtattct tgttctttga ttttcgctaa gatccgttcc ccttcttttt cttttacttg
     2701 tctcatttgc gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc
     2761 gatttttgca taggcattca gccgtttgac atactcattg ataccttgga tcaggtattt
     2821 ttcttttaat tttcctactg agataatttt gatattcatg ttttctctcc tttggtcttc
     2881 atgctcactt tgtttattat aatcaatttt ttcgtctata tgtttatttt ggctattttt
     2941 gttaaaaaga tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag
     3001 tgttatttta ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat
     3061 ccactttata cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt
     3121 agttttacac agtatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt
     3181 tttcctaaag tttttcataa aaagttcaca gtctcaaagt agactaaagt caacaaataa
     3241 gactatacta acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa
     3301 tgtgacacca aagatgaaaa aaagtaaaaa taatagcctc tggcgtaagt taggtctggg
     3361 gcttgttgga gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg
     3421 cactggcaac aatagttcta ctgctacaag cggcaatcag aattctgccg gtgaaacagt
     3481 tgtggaaaat gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca
     3541 agacgctgtc gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg
     3601 actattcggg cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag
     3661 tgaaggaagc ggcgtgatct acaaaaaaga cgggaatact gcttatgtag tcacgaataa
     3721 tcacgtagta gatggccagc aaggtcttga agtcttgatg aaagacggta cgaaggtaaa
     3781 agctgaatta gttggaacag atgcttattc agatttagcg gtgttgaaga tcaattcaga
     3841 caaagtcgaa acagttgcct cttttggtga ctcaagtgct ctgaaggttg gtgagcctgc
     3901 gattgcgatt ggttccccac taggatctga atatgcaaat tctgtcactt caggaatcat
     3961 ttcttccttg aaccgacaag tgacaagtac gaatgaatcg aacgaaacag tcaatatcaa
     4021 tgcgatccaa acagatgcag caatcaaccc tggaaattct ggtggtcctt tagttaatat
     4081 cgaaggacaa gttatcggta tcaactcaag taagatcgca agtacttctg aatctacttc
     4141 taatgtaagc gtagaaggaa tgggatttgc tattccaagt aacgatgtag tgaatatcat
     4201 caatcaactg gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga
     4261 cttatctgcc gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac
     4321 taacggtgtc atcgtgacaa atgtccaaac agctacaccg gctgaaaaag ctggattgaa
     4381 acaatacgac gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca
     4441 atctgctttg tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc
     4501 gaaagaaaaa acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca
     4561 aaatagtgaa aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt
     4621 tggaaatgct tctccgacta cggcatcctc aggcaatact aagattcctt tttttgctgg
     4681 agcatttggc aaatgcaatt cacgagcgga acagatcatt ccatagctat ctacccctcg
     4741 caaagttcct ggccagatca tcattccatc tggcatcata gctcctggtt ttgcaacgac
     4801 aactttttgt ccagcttcga tattgggtgc accgcaaacg atttgcaata ctgtaccatt
     4861 gtctacttct gtctcagtaa tcgataaatg gtcagaatca ggatgtggga cgcatgtttt
     4921 tacgtacccg acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc
     4981 ttctttgatc aatcgattca atttcgctaa ttcctcatct gttaagctta cttggccgtt
     5041 cccttcgatt gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg
     5101 ttcatctttg atccgagcaa cattccattt gcgttcaaca gtgttttctg ttcctttatc
     5161 gtcggctata ataaccatca aggtgtctcc gacgtgtgct ttgttataag caaaaatcat
     5221 gttctttcct ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca
     5281 tgaaattttt ttcatgcttt atttccgaag aagcctcttc tggaacacca tttattcgta
     5341 acaagggact gtgacaagac ttttgtcaca gtccctttac tgttttagtt aattgattaa
     5401 aaaggtcgta caagttccac ttgcttaatt acttggctgt tcttcttcgt cagaaacgat
     5461 caagacatca caaggtgaat gacgtatcac gtagctggct acacttcctg tcataaagcg
     5521 ttcgactgca tttagacctg actgcccaac catgattaga tcgatctcat atttttttgg
     5581 caatttgact gccatggatt cacgaacaga accatatgca ataattcctt ctatatttgt
     5641 gtaatcaaca gatttcccat attctttaca ttcttcgatc atttccttgg ctgtttcagt
     5701 ttgctgatcg actagatttt gatcaatagg tgcatagccc ataaagttat aaaattgctg
     5761 atcgattacg tttgctacat atactttccc attattacgg cgagccactt ctaccgcttt
     5821 tttgaaagca gcaagtgctt gttcactacc gtcgatacct actaaaatat tcttatattc
     5881 ttgattcatc ttgacccctc ctaacagatg tcagcctttt gttacacttt ctaggaacga
     5941 ttcgatttcc tgttttgttt tacgatcttt attcactaga cgacctaatt cttttccgtc
     6001 ttggatcacg acaaaacttg ggataccaaa aatgttccat tcagcggcta catctatgta
     6061 cttgtcacgg tctacttcaa taaattgcca atcagaaaag tcattttcaa tttcaggcat
     6121 ttgcggtttg ataaaacggc agtctccgca ccagccggca gtaaagaaaa atacactctt
     6181 tccagtttct acgtagcctg ctaattcttc taaactattt gggataatca ttctttgtcc
     6241 tctactttct gtttatgtat ttttcaatta aattatacac ctttctttcg aactaacaaa
     6301 cattgatcct ctttttataa aaaaaatctg gcaaaaagca atcttttgcc agattttata
     6361 gtttcactat cgcattattt tccgtatacg atcgtattta ctgttgattt atccaaacct
     6421 ttcagtgttt gaatcaacat ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt
     6481 tgatgcgtat ggatataacg tcccactacg ccaatcactg tacttgggat tccttcgttt
     6541 tgtgtatgag cagcacctgc atctgtaccg ccttttgaaa caaagtattg ataagggata
     6601 ttgtttgttt cagcgatatc cagcaagtac tcgcgcaaac gaggaagcat gatcaaacct
     6661 ggatcttgga tacgcatcaa tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc
     6721 actgtatcat ctgctgcaga gcagtctact gcaaagaaca gatctggctt gaatttattg
     6781 acagaagctt tagaaccacg caagcctact tcttcctgga cattcgcgcc tgcaatcaat
     6841 gtatgaccta atgtttcatt ttgcaacgct tctaaagctt ctaataccat tgtacagcca
     6901 tagcggttgt cccatgattt actgataata ttctttccgt tggctgtttt gatcgtttca
     6961 gcgtaaggaa cgattgtgtc tcctggaagg acaccgtaac tcattgcctc ttctcttgat
     7021 tcaaacccag catcgaacaa aatatcggtt acttctactg atttttgtcc gcttgttcct
     7081 cgcaataaat gtgggggaac ggaagaagaa atacatggat aattcttacc agtagaggtt
     7141 ttaagtgtaa aacgctgagc agaaacaacg taaggattcc agccgcctag aggtacgact
     7201 ttaaaaagcc cgttttcttg gatttgtgtc agcatgaatc cgacttcatc catatgtgct
     7261 gcgaccatta cacggggtgc atccgcttct tcatgatggc gcagaccaaa aatgccgccc
     7321 aaaccatcta actgtaactc atctaccagc ggttccatat gactttccat ataagcgcga
     7381 acatcttgtt caaaaccgct tgttccttgt aattctgtta actctttgat tcgttgaaat
     7441 gttttttctt ccatatgcta ttcgtctaaa tttcagacta aactcctctc agacttatat
     7501 tgatctgcgg ataaaatatg attttttctg cagaccgatc gtatttatta tagcttattt
     7561 tttctggtag cggttattta tttcaaagaa aatatgagag tgtggtaaaa taagcacaag
     7621 gagtaacaag tgagaagaag gaggtatgtc cacatgtatg aagaaagcga actaagctat
     7681 tttaaaggcg ggttagcagt tggaatcggt ctaggattat tgagcggggt agcttcaacg
     7741 atttggtatc atcgcaaaaa agtattgtca gcagaccaag tacttgaaaa tatcaagtca
     7801 tcttttctaa aagaaggtgc aatcgaaggt tcttggatct cttttgaaaa ggaacctatt
     7861 aggaaattcg ccgttcggtc gagtgcttat cggggaggtc tttctcgaat agaagatggc
     7921 gaaactgttt attatgaatt tttagcagat gctcatacag gaaccgtatt agaaatttca
     7981 agaaaaaaag gtgcaacaga ttaatacgtc aaaaaagtga gagaacgaat acgcacccac
     8041 tattcaaaag agaggtatca tgtccttctt ttgtagtggg tggtttttta gatagagctt
     8101 tccaaacata tattccagta ttttcttttt aaataaatga tatttagcgg gaaaaactag
     8161 catttccttt gattatgagg tatttttaat ttagcttttg ttttctatag ttaaggagag
     8221 tgaaaaatgg aacaacaaga aaaaattgct gttctgattc cttgttacaa tgaggaagca
     8281 acaatttcaa cagtcattgc agactttaaa cgagaattac cagaagcaga tatttatgtc
     8341 tatgataata attcgaccga tcgaacatac gaattggccg ttcaaggtgg agcaatcgta
     8401 aaaaaagaac ctcgtcaagg aaaaggaaat gtgatccgcc aaatgttctt tgatatcgat
     8461 gccgactatt atttaatggt agatggcgat gatacttatc ctgcagaagc tgtacatggt
     8521 ttgctcgaga aattacgttc tggtgaagca gatatggtca ttggtgatcg cctatcaaat
     8581 ggaacctact ttgatgagaa taagagacct ttccacgatt ttggaaataa ccttgttaga
     8641 aatacaatta ctagaatgta taaaacaaaa attcttgatg tcatgaccgg ctatcgagga
     8701 ttcaatcgta tctttgtcaa aagttttcct attatgagtt ctggttttca aatcgaaacg
     8761 gaattgacga tccatgcttt agataaaaaa ttcaagttcg ttgaaatccc aatcgattat
     8821 cgtgatcgtc ctgaaggcag cgaatcaaaa ttgaatacat tctctgacgg attcaaagtg
     8881 atcatgatga ttattaaaat gtttaaagat tataagcctt tgcttttctt tgggatttgg
     8941 accatcttct tctttttatt tggattggtc acggggattc ctgtgattcg cgaatatatg
     9001 ctgacaagct ttattacacg catcccttct gcgattttaa gtactggatt gatgacttta
     9061 gcacttcttt cattagtaac agggttgatt ttggatacag tcgtcacgaa cgctaagaaa
     9121 gaatatgaat tgaatctata tcatgtttat catgaatacc acagtagaaa gtaaaaaaag
     9181 gatcaagact gcaaaaataa tttgcaattc ttgatcctct ttttaattaa ttatttcgcc
     9241 aaacgataga tggcatctgc atagattgct gttgctctga acaagtcatc aagactcata
     9301 aattcgtttg cttgatgcat tgtatcagta tatcctggga acatcgctcc ataagcaacg
     9361 ccacgtttca acaaacgtcc gtaagttccg ccaccgatga tttgttcgta gcctttttct
     9421 ccagtgtggt cttcatagac ttgcaataat gtagccacca atggatcatc cattggtacg
     9481 taatgtggtt ccattacgcg agcacctcga gtaactgttg cgccttcttg ccccactgtt
     9541 tgttcgatgt cactttgcaa tccgtctact gttacacctt ttggaaaacg gaaattcata
     9601 ttgatgaagt tcgcttcctc tgtaccattt tcttcaaaag caaatagtcc agcattcatt
     9661 gtcaatttgc ccattttttc atcttcataa gcgactccta gtttttctcc ataaaaatct
     9721 tcatggacat aagcagctgc tacatggata aattgtttag cagatcctag gaattcatag
     9781 ttatctaaga acaatgccaa gaaagaacct gcattgatac cagattgtgg gcttgcacca
     9841 tgtgcgcctt taccaactaa ttcgattttt acatacgtat tgtccgcttc aatcgttccg
     9901 cttactggat ttgcttcgat gaattggtaa aatgcttctt ccatcgcgat agctgcatct
     9961 gcacttggta cttgaagtgc tgcagttgct gttcctggca ccatattttc gcgaagtccg
    10021 gaaacaaaag atttcaagac ataatctcct gcattgtcgc ctttgaagcg taaagctaaa
    10081 gaaacatttc ctttttcacc attgatgatt gggaattcag catctggtga gaaaccaaaa
    10141 tctggttctt cttcatgttc gaaataataa gccatatcgc cccagccgct ttcttcatcg
    10201 ctgccgacta cgaagcgaac tttttttgat agtttcaagt caagttcttt aatgatcttc
    10261 atcgcatagt aagctgccat acttggacct ttgtcatcgc ttgatccgcg tgcaaagatc
    10321 tttccatcct tgattactgg ttcataagga tctgtgtccc agccatctcc tgcaggaacg
    10381 acatccatgt gtccaaatat ccctagcgtt tcgtctcctt cgccaagatc aatatgccct
    10441 gcatagttat cgacattttt tactttaaat ccatcacgtt cgccataagc taacatatgt
    10501 ttcaatgcgt cgcgaggtcc aggtccaaat ggtgcatctg gtgttacttt tgagtcatcg
    10561 cgctcgctgt tgacacgtaa aagatttttc aagtcttcaa gcaagtcttc tttacgtgct
    10621 tctacttctt tttgccaatc aattgtcata aagttaatcc tccacttatt taagtcttcg
    10681 tgttcatgat accacaaatt acagaaaaaa atagttcaaa aaatctgaca attccttttc
    10741 ttatctgttt ttctctaaaa aaaatatacg ctataatgag taaaaggagg tttttagatg
    10801 ctgataaacg aacaactagt gaaaagagtc atcacacctc gtccaaaaat ttcacataaa
    10861 ggaacatttg gacgtatcgt tttgattggc ggcaatgaac aatatggcgg agcaatcatt
    10921 atgagtgctt tagctgccgt acacagcgga gcgggtctca cgactgtagt aactgatgaa
    10981 aaaaatcgga gtgctcttca cagtcatctg ccagaagcaa tgattgtagt ttggacggat
    11041 actgaccagt ttctttccgt aataaagcaa gcagatgtac tcttgattgg tcccggattg
    11101 ggtacttctt ctaagagttt ggaacgtttc aaacaagtac tagaaaacca atcaaaagaa
    11161 caatggctgg tgattgatgg ctctgcttta agtttacttg ctgctaatca tcttttgcta
    11221 ccctatcccg aacaaactgt acttacacct catcaaatgg agtggcaacg gttgagttct
    11281 ctaccgatac aagaacaacg ggataaagaa aacaaaaaga agcaacagga aatcggtgct
    11341 gtgctcgttt tgaaaagtca tgagacaaaa atttatggtc accagcactt ctacaccaat
    11401 cctttaggtt ctgctgctat ggcaacgggc ggcatgggtg acactttagc tgggatgatc
    11461 acaggatttt tagcccagtt tcctcaaaat gaccaaacga ttgcagcagc tatttatctt
    11521 catagtttca ttggagacca gctggcaaaa gaacaatata ttgttttgcc gacacaaatc
    11581 agcgaagcac ttccgaaatg gatgaattat ttttgtaaaa aatcaatgga aaacgaggca
    11641 tgagtcatgg agaaatttac aggttatgtg cgagaacctt tttattatga aacagatcaa
    11701 atggggatta ttcatcacag caattatatc cgctggtttg aagaggctcg ggtagcctta
    11761 ttggtacatc tagggtttcc ctataaaaaa atcgaagaat ctgggatcat catccccgtt
    11821 ctggaagtaa attgcagcta taaagaaatg atccattacg gagaaaaagt gagcatccat
    11881 ccaactatcc aaaagtatac ggggacacgg ttggatttta gctacgaaat tgtgggaact
    11941 gaagacaaaa aattaaaaac tactggcagt agtaaacact gttttttact ttctgattcg
    12001 caacgattag tgaagctgag caaagtcaat ccggagttag atcagttatt ccaagcgtac
    12061 gcttctttgg atgatgtaaa ttcttaataa agaccgtcta agcatttttc ttgtattgtg
    12121 tcgagtagtt cgtataatga aaagaaaaaa gcaggtgatc cccatgtatg tcgttaaagt
    12181 aatgcacggt tacatcgata aaactggctg tcgcactcgg gaaaaaaact tagacaatct
    12241 gctcattttc aaagacaaaa aagaatccga agcttttgct aaaagaatcg gcggtcgtgt
    12301 caaaccgatc caagaggtac gaccagattg attcaatatt tttcttttcg aggctctaat
    12361 aggcatgcct atcagggctt tttttgcaga ctgtgttttt atgcccacac gatttgtaag
    12421 cgatttatgt tataatagat tatatctgca tagaaaggaa ggttaaatgg ttatcaataa
    12481 tttgcaacag caagcttatg aatctatacg taaacaaatc atttattccg agttagcacc
    12541 tggaagtaaa atctccgata aagtgttaga agaacaacta gctatcgggc gtacaccgat
    12601 tcaagaagca ctgatccaac tgcgcaatca agagcttgta aaaaccattc cacagtccgg
    12661 cacttatgtt tcaaaaatcg atatccgatc tgcttcatta gcccgttata cgcgggaaaa
    12721 gttggaaaaa ccgatcctgc aagaatgcag tgcgaaaatg accaagcaag atcaagctaa
    12781 actagaaaaa atactagaac aaaccgatca ggcagtaatc gctcaaaata aaaaagcttt
    12841 ttttgatttg gatactgatt ttcaccgaac ttgttatgaa attgctggaa aacgagaaat
    12901 ttgggattgg ctagaaagtt atagtacgca cttgaaccgt ttcagatggt tacgtttgac
    12961 gatttcagaa ttagactggg gtcgagtatt agatgaacat cagacaatgc ttcaatcaat
    13021 gattgatcat aactttgatg aagtcggatt tctttgcacg atgcatttac atatgattat
    13081 cgaggagcaa gaatacgtga ttcataatta ccccgattat tttgaagata ttcaggacag
    13141 acccatcaaa taagaggttt tggaatcagc gttttgacct gagtattaga gaaagaaacg
    13201 gcaaaatagt tcctcatatt ttgccgtttt ttttgagcta ataccgaggg tcagctgatt
    13261 gcacaccgtt tattgagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
    13321 aagtaaaaat agcttcttat gtttttaact tggctggact tattaccgca gatgcaagct
    13381 tttgaacacc gttttccaag aggtcgtgag cctgccgttc atctccgagt cacaaggaac
    13441 aatttcaaaa aacagcttct catgtttgtt gaatattttg acttgtgacc gaggagttga
    13501 caggagaaca tcgtttattc ggaaaaaaag gactgtgaca cgacttttgt cacagtctct
    13561 ttatttgtaa tctaaatcat actccgtaca tcttttttct atttttttta tttcttctaa
    13621 gatcccttgg ctacgaattt cattatatgg ccaaagtaag actgcctcta atacataagg
    13681 cgaaaaatcg ctttgattca tctcttttga aaaactcatc gctgctatct cgctcaatgc
    13741 acgaatgttt gattgatgtg tataaaaagg caaccgccac agcacaattt tttcgctttg
    13801 cgtataaatt cccttaacag aagcttcgat gtggtgaaat agttcatgtc caataatcaa
    13861 agcttgcgct gtttctttag agatcgaagc tagtcctgaa cattttaatt tttctaacgg
    13921 ctccgtcatc aatcgaatcc cttcatcttc tgcataagtt gcaaaagtga ttcgttcgtt
    13981 tatttgctct gattcacagg aaacgattgg aacagccatt tgttcacaaa tttctcctac
    14041 atcggcaaaa gggtactttt ttttgatgct acatgccgtt tcgataccac aatcgattga
    14101 ttgttgtata agaattttct gcttggacaa aggaatcttt ttccaaaata aatctcgccg
    14161 aaactggtag aatccccaaa tttcttcggg gatctgttgt aattcgttta gtaactcttc
    14221 taacttatgt ttgtttgata tttccggcaa cgacgatcac ttcttcctcc ggttccatag
    14281 tagatacttc cagatccatc aaaagctgta gttgttccag atccgtcgct gtaaaatcag
    14341 agactcttgc tcctaagcag agataaaatt ctggtcgggc aatcaattca gtttgataaa
    14401 ctgccatatc ttcccaaagt tgttcgacag ctgtcatata attggctgca gttgttttca
    14461 tacatgaagc atgtccacgt tggaccttga taaatccttt ttgatcgatg atccgaagac
    14521 ttccagcatt ttttgaacga ttctgttcgc cgcatacata aaagaaaggt gtattttcca
    14581 ataagcaaac ttcattttta tttaatcgca tatcctctgc agcaagctct agtgcttctt
    14641 ctgttgtgat ctccttggtt agatcggttg ctttcacttc cgtagaacca gtagcgatag
    14701 ccgttacttt tgaagtctgt ggatcgattt ctacgtggac ttcgatcgat tctggtgtag
    14761 cgcctgattc aatcgcttta ttcatcgctt catttttcaa cgatcgtata tcttctttac
    14821 ttggcgaagg gatgatacgt tctacaacat ctcggaccat tgcaagtgct acaccaatag
    14881 aagaaatcac ttcggcgttt tcaggaatcg aatatttcac acccatttta ttactaaagt
    14941 aagtaatcaa tgaggcagcg ccgcccccta cacctacaag agaaatctga tctttctcca
    15001 agtgatactt gtctgccaat tctaaaatca ccggttcaat cttagcatag gatttttcca
    15061 tgatctgttc tgcgatgtct tcaaccgtcg tatgacaata atcagctaat gcttggatgg
    15121 cttttcgtgc agatgggaca tttccataag aaaaatgttc ttcttgcact aatccaagga
    15181 cattggctgc acatgtattt gtaatagtca cttcttctcc gtcttccatc actaccttga
    15241 cgtaatcagc agggtctcct tctttaggag agaaaaattc aaccttagga cctttgattt
    15301 tttctgtctc cgtaaatact gcataatcta gaccggcaat atgtgcagaa cgtggaccga
    15361 catcgatgat tcctgactga ttagcccgta ccatcgaacc gcctgccaca cctagaaccc
    15421 gaacatctaa tgaagagata tatgttgggt ggccacctac aattgaataa tcgattgctg
    15481 gacgtccatt tttaatgacc ccgatattgg ttgttgttcc gcctacttca aaataaacac
    15541 cattagacgc tctcaagtac atcaaagagc ccatgactga tgctgctgga ccggaaagca
    15601 tggttaaaac aggtcgtttt ttcatttcat tgatttccat tactccgccg tctccgcgca
    15661 taatcatcaa agaaacattg acccctgctt ctcgtacgga atcttctgta gaggttgccg
    15721 tatctagcat ttttggtaaa atagatgcat tgattgctgc tgtccgagtt cttcttgtca
    15781 atccatataa ttttgtgatg tcagaagcca tcgttgtagg gatcgatttt accgatgctg
    15841 cttcataaac aactcgttct ggttccccat tatctactcc aaatgccata gaagaaacca
    15901 agacctccgc tctttctctt tctaaagaag aaatcgtttc tgaaacacgg tctacattta
    15961 agtgtttgac aggtaagaat gcattgacaa tctctatttt tttcttattc cctaaatcga
    16021 tatcgttcaa tctagtttgt cgttttgcta aaaaaccttc taatccacct tttgccatcc
    16081 cgattacgcc gacttttgct acgtctcctt caatcagtgc attagtcgct tgtgtggtag
    16141 aatgtgcaac aaacacaaca tcttctggac tgatattatt ttccagcaaa caattttgaa
    16201 aagactgaac gacacctgcg gctactccac ggacatcatc gtgggttgtt ttcactgaag
    16261 attttccgat gatttcgtgc gtcgcattat cgattgcgac tgctttcgta tgtgtaccgc
    16321 caacatcaat tcccattcgc acaaaacgtt tcattttctt ttcctccatt catctaatcg
    16381 tttttatcga cgttttgttt actaggcgtt cccgtacaat aagaacgtga tcaggcataa
    16441 gatcgctccg atgatccaac cagttgggat ggatttcttc atatattctc gagaagttac
    16501 ttttgtataa cctaatcccc aagcaaccca agattgtgta atatctaaat gctgaggagc
    16561 aatcgttgtg attgcaaata acgggaacaa aaaggctggc gacatcgttg gattaacagc
    16621 taatacgaca gctaaaatag ctgagcctga accgactaga ttcattggac cgcggaaaaa
    16681 tcctagtggt gtcaatatag caaatacgat tgccaagact aatggtgatt gcggtatcca
    16741 atctccaaaa atagctgaaa aataaggaga agcatacgca gctgaattat tgaacatcgc
    16801 taaggtcatc aagaaaccaa ccattggcgc aacatctact gcgccatctg taaataattt
    16861 cgcaaactga cgacagattt ctttataacc tccgcgcaat tttccagtaa acagcaatgc
    16921 ccagatacct gctagaataa aacctaagat gattggaaga tctaaaagaa cgacccctaa
    16981 aactggtaat aatacggcta accatgagat cattggtgcg tcgccacctt cagctggtac
    17041 gtttgctgcc attgcatagc gtttcttttt gttcattgat atattagcga ccgttagtac
    17101 cacaactaag cttacaatca tcccaatcat accaatttgg aaatagtcat tataagtata
    17161 gctttcagcc gctggattga aaccagcata aatcgtttga tattgtttga aattgacgat
    17221 attagcaaag attccagcca taatcgagcc cataaatgag aacagtgcaa ccggtactga
    17281 aatacctaat gacatcaaga ttggcaacac aataactgca atagaaatca ctggtccgat
    17341 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
    17401 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
    17461 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
    17521 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
    17581 cgccgggttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
    17641 gatggcaaag ccgaccatca agttgtagcc ttttacacag taccagacca tgaaaaagaa
    17701 tgagataact aacaaaatac caataataat atcactcatg ttattcctcc gtccattttg
    17761 tttttctgat aagcaaacta atatatcagt caaactcatt ctaacttatg tagttagcgc
    17821 tttcattaaa gggtgaccat aaatgttcag ttttcaatta aaaaacctta attatgctat
    17881 tttagactta tacgttattg tcctgtttca tttgatggag gattgtaaat aaaaaaagag
    17941 gaaagaacat aacttttcgt tatcttcttt cctctttata attgtttagc ttatttagcc
    18001 aaagcttctt ttgcttggtc agccaatgca gtaaatgctg ctgcatcgtt aacagccaag
    18061 tcagctaaca ttttacggtt gatgtcgatt tcagcaactt tcaaaccgtg cattaatttt
    18121 gagtagctca agccattcat acgagctgct gcgttgatac gagcaatcca caatttacgg
    18181 aagtcgcgtt tcttttgacg acgatcgcga tatgcgtagt tgtatgaatt cattacttgt
    18241 tcttttgctg ttttaaataa agtgtgtttt gaaccgtagt aaccttttgc taattttagc
    18301 actcttttac gacgtttacg agttactgtt ccacctttaa cacgtgccat gtataattcc
    18361 tcctaataaa aataaatctt cagattctgt tatttgtact tcttatttca tttttgctag
    18421 ttgttggcga atacgtttga agtcgccact agaaaccatg cttgctttac gcaattgacg
    18481 gcgttgtttt tttgttttgc cgtggaaacg gtgacttgta aacgcgcgga agcgtttcaa
    18541 tccgccttta ccagtacgtt tgaaacgttt tgctgatccg cggtgtgttt tttgttttgg
    18601 catgactata tttcctcctc aaaatctttc gttatccgac tatcggctgt ccaacagcca
    18661 gttagtcatt caaattactt actgtcgttt ttcggtgcca gcgtcaagaa catgctgcgt
    18721 ccgtccattt tcgctttttg ttccactgtt gcaatatccg cagtttcttc agctaagcga
    18781 ttcaagactt tttgaccaat ttctttatgg gtaatggcac ggcctttgaa acggatagaa
    18841 gctttcactt tatcgccctt ttccaagaac ttacgtgcat tacgaagttt tgtattgaag
    18901 tcattcaagt cgatcgtagg acttaagcga acttctttta cattgatcac tttttgtttt
    18961 ttacgcgctt cgcggtcttt cttttgttgt tcgaaacggt atttaccgta gtccatgatt
    19021 cgcgcaactg gtggtttcgc accaggggca actaacacaa gatccaagtt tgcttgttct
    19081 gcaatattca atgcttctgc ttttgtctta actcctaatt gctcaccatc tgaaccgatc
    19141 aatcgtaact ctcgtgcacg aatgccgtcg ttaaccatca tatcctttgc tatggtcatt
    19201 cacctccaaa tattatgaga gaaagaaaag cttaccgaat aaacggcaat aaaaaaacga
    19261 gttctcttac agaacccgcg cgtacttcac ctaatagaac cagtgaaaac tatctagccc
    19321 agtgacatta tcaactaagg cgagaagcgg gtgcttctgc ttttatttct caaccatcat
    19381 agtatagcaa aaactaatct ttttgtcaat gtttttcata cagtttgtct tccttttctg
    19441 aaaatgatta gattagcaga ctttcttgca gtgattcccg cttctttgct atactaaaga
    19501 aaaacgaggt gaacccacat gaaaaaaatc gaacctattc caccttctct tcctcttctt
    19561 acaaagggag agttttattt agaagatgag gagatcttct ctggtcttct gatcaacggg
    19621 ggtgaccaaa gctatctcga tgccaaaaat atggtattaa gagagagtca tatccaaaaa
    19681 cttacgatgc aaaaaacagt tttacgtcat tttgaatgca gtaatgtcat ttttgaaaaa
    19741 tgtgattttt ccaatctgga gtggattggt ggcagtttcc accaagtcgt gtttcatcaa
    19801 tgtaaattaa caggtacgaa ttttgccgaa agttatcttc gagactgtac tttcactgat
    19861 tgtattgctt cgatggcttc ctttagtagt acaaacttga aagctgtctc cttcgatcat
    19921 tgtcaattag aagacagcga attttatgag gtcacttgga aaaacctttt tttgtctgac
    19981 aatcagttga ccggttcgaa ctggtttcga acttctctga agggattaga ctttacgact
    20041 aatttcttca cagcaatcgc tttatctcaa gattctttgg cgggactaat cgtcaaccaa
    20101 gagcaagcac ttgtgatcgc ccaagccctt ggtttgcaga tcaaagacta gatcaacgaa
    20161 tccgccgcca cagtgcaata agcatccaaa tagataagac caaactgatg atcaaggcga
    20221 ttccccatcc gaaaacggaa tgggtaaagg gtaactggag attcattccg aagaatcctg
    20281 tcacaatagt tggaacggtc aagatcagcg accagaccgt caaaaatttc atcgtgtcat
    20341 tcaagttgtt attcagcaaa ttattataag tgccagagag ctggtctaaa atctgcgaag
    20401 ctaagttcgt catttcaact gcttgtcgtg cttcgatcag cgcatcgtcc aactcttctt
    20461 tttccttttc tgacaacttg cgataaatag ccaatgcctt gatttgttct aaaagtacta
    20521 cattttgttt tgttcctgta actaagtaaa ccaaaccgat ctctaaatct gaaagttgca
    20581 gcagattctt gttcgttgtt ttttctctta atttcagatt aagccgctga cgttcactat
    20641 tcacttcctc gattaaagga aaaaaggaat cagaaatcaa aaacaatgta cgaaacaata
    20701 aactgtatac actcatgtct ggcattcgtt ccaaaagcga tcgaattagt cgaatcacgt
    20761 actcggtatt cggcgtggta aatgtaaaca aaccgtcttc ttttaaaata aaagcgatag
    20821 gactagtttc ataatgatta tcgacttttt gctggtgcgg aacatttgat acaagcagta
    20881 acgcttcatc aaaagaatcg tactctacgc gcgcccgttc atttttatct agagaataag
    20941 tcagcatctc gtcactaatg ccatattctt cttttaagtt tttaacagct agcacatcgt
    21001 tttgctgtac attgatccaa aaattacggt cttgattaaa atagtgtttt tcccgcatac
    21061 tctatcctcc tgacatttgt ttccagtata aagaaaaaag tcgctcaaat aaaggtttac
    21121 cctttatttg agcaactgta aatcaaatgg ctactttggt ttggcgaatc tctgctaatt
    21181 tttcttcgat cattttcaag acttcttctt ttgcttgagg atcttccaca aaattatagc
    21241 ggtctccatc gatttggatt tttggacttt ctttgtattc ttcataccat tgatcgtaac
    21301 gtctgttcaa ttctttgtaa taatcgtata gacttggatc aaaggacaat tgttcatacg
    21361 aacgaccacg tttttcgata cgctccaaca tcgtatcaaa agatacacga atatgaacta
    21421 ataaatccgg atgtttttta tgggcagcat agggaagttc ttccatcatg ttttccagca
    21481 gttcatcata cacacgaact tctgtttctg ttgctcttcc tagatctgca tttagatgga
    21541 aaagcagtga atcttcataa atcgaacggt ccaatacatt attgtcatct ttcattgctt
    21601 gtttgatgct tgcaaaacgt ttattcaaaa aataaatttg cagcaaaaaa gcatactgtt
    21661 cagggtttgc ataaaataag ggcaagactt cattatcatc aatcgattca taaaatgcct
    21721 gactatcaaa gtgttcagcc atcatttctg ttaaacttga ttttccggca ccaattgtac
    21781 ctgctaaaac gatcacgctc atttacctcc agctcacttc atcactaaat attgtgttta
    21841 actccaacct ttttaaatat acccccaata tctagtagtg tcaattgtat ttttttctag
    21901 tcaaagaaga aaaaaagtca aaaaattctt aagaaaaaag tgttttttct ttagaaaacg
    21961 tattcttagt tttacttctg agatggtttt agtaaaataa aggaggaatg ttttttgaga
    22021 cccttagtgg acaaaaaaat tcgtgaagca gcgcaactcg ttttgggcgc tttatttttt
    22081 gcttaaaaat cacttttacg tgagaaggag ttagattatg gcaaactatg gccaaggaga
    22141 atcaagcgga aagatcatat tgatgggcga gcacgcggtt gtttatggag aaccagcgat
    22201 tgcctttcct ttctatgcaa caaaagtcac cgcattcctt gaagagctgg atgcaatgga
    22261 cgatcaactg gtttcttcct actattcagg aaatttagcc gaagctcctc atgcattaaa
    22321 aaatatcaaa aaattattca ttcacttaaa aaaacagcat gacatccaaa aaaacttgca
    22381 actgaccatt gaaagcacga ttcctgctga acgtggaatg ggatcaagcg ctgcagtcgc
    22441 cacagcagtc actcgtgctt tttatgatta cttagcattt cctttgtctc gtgaaatact
    22501 attagaaaat gtccagcttt cggaaaaaat cgcccatggt aatcctagtg gaatcgatgc
    22561 agccgctact agcagcttgc agccgattta ttttacaaaa gggcatcctt tcgactactt
    22621 ttctttgaac atcgatgctt ttttgattgt cgctgataca ggaatcaaag gacaaacaag
    22681 agaagccgtc aaagatgtcg ctcacctctt tgaaacccag cctcatgaaa ctggacaaat
    22741 gatccaaaaa ttaggatact tgacgaagca agcaaaacaa gcaatcattg aaaattcacc
    22801 agaaacgttg gcacagacaa tggatgaatc acaatcactt ctggaaaagc tgacaatcag
    22861 caatgatttt cttaatctat tgatccaaac agcaaaagat accggggcat tgggtgccaa
    22921 attaactggc ggtggacgcg gtggctgtat gattgcatta gcacaaacaa aaacaaaagc
    22981 ccaagaaatc agtcaagcac ttgaagatgc gggtgctgct gaaacttgga tacaaggatt
    23041 aggagtacat acctatgttt aaaggcaaag cacgcgcata taccaacatt gctctaatca
    23101 aatattgggg taagaaaaat gaagaactca tccttccaat gaacaacagc ctttcgttaa
    23161 cattggatgc attttataca gagacagaag tcatcttttc tgatagctat atggtagatg
    23221 aattttatct agatggcacc ttgcaagacg aaaaagcaac aaaaaaagtc agtcagtttc
    23281 ttgacctttt tcgtaaagaa gccggcctct cactaaaagc ttcagtaatc agtcagaatt
    23341 tcgttcctac cgcagctgga ttagcctcct ctgccagcgg gctagctgct ttagcaggcg
    23401 cttgcaatac tgctcttaag cttggattag atgatctctc tctttcaaga tttgctcgac
    23461 gcgggtctgg ttcagcttgc cgaagtattt tcggtggttt cgtcgaatgg gaaaaaggcc
    23521 atgacgactt aagttcttac gctaagccag tcccttccga ttctttcgaa gacgatttag
    23581 caatggtttt cgttttgatc aacgaccaga aaaaagaagt gtccagcaga aatgggatgc
    23641 gtcggacagt cgaaacatcc agtttttatc aaggctggtt agattccgtt gaaggggatc
    23701 tatatcaatt gaaacaagca atcaaaacaa aagatttcca acttctcgga gaaacgatgg
    23761 aaagaaacgg actaaaaatg catggaacga ctttagctgc ccagcctcca ttcacttact
    23821 ggtctcctga cagcttaaaa gcgatggatg cggtgcgaca attgagaaag caaggcattc
    23881 catgctactt tacgatggat gctggcccta atgtcaaagt tttagtggaa aacagccatt
    23941 tatctgaggt acaagaaaca tttaccaaat tatttagcaa agaacaagta attactgcac
    24001 atgctggtcc aggaattgcg attattgaat aaagggagat ttttatgatt gaagtatctg
    24061 caccaggcaa actctatatt gccggagaat atgcagttgt tgaaacaggc catccagcag
    24121 ttatcgctgc agtcgatcaa ttcgtgacag tcactgtaga atctgcacga aaagtcggaa
    24181 gtatccaatc tgcacaatat agtgggatgc ctgtacgttg gacaagacgc aacggagaat
    24241 tagttttaga tattcgggaa aatccttttc attatatcct tgctgctatt cgcttgactg
    24301 aaaagtatgc acaagaaaaa aacattcttt tatcctttta tgatctgaaa gtaacaagtg
    24361 agttagacag ttcaaatggc cggaaatatg gattgggttc tagcggtgcc gtgaccgttg
    24421 caacggtcaa agccttgaat gttttttacg cgttgaattt atctcagttg gagattttca
    24481 agattgcagc actagccaat ttagcagttc aagataatgg ttcctgcggc gacatcgctg
    24541 ctagctgtta tggtggctgg atcgctttct caaccttcga ccatccttgg ctccaagaac
    24601 aagaaactca gcattctatc agtgagttac ttgccctgga ttggccaggt ctatccattg
    24661 agccattgat tgctcctgaa gatttacgtt tattgattgg ttggacgggt agccctgcct
    24721 ctacttctga tttggtcgat caagttcacc gttcgagaga agataaaatg gtggcttatc
    24781 agcttttctt aaaaaatagt acagaatgtg tcaacgaaat gatcaaaggg tttaaagaaa
    24841 ataatgtaac gttgatccaa cagatgattc gaaaaaaccg acaattactg catgatttat
    24901 ctgcaatcac tggggtcgtc atcgaaacgc ctgctttgaa caaattgtgt aatttagctg
    24961 aacagtatga aggagccgca aaatcttctg gtgcaggtgg gggcgattgc ggaatcgtaa
    25021 ttgttgacca gaaatctggc attcttcctt taatgagtgc atgggaaaaa gcagaaatca
    25081 ctccactgcc gttacatgtc tatagcgatc aaagaaagga aaaccgatga atcgaaaaga
    25141 cgaacatgtt tcactagcta aagcctttca tgataaacaa aaaaacgaat ttgacttcgt
    25201 acggatcatc cataaccctt tgccgcaaat agcagtttct gatgtcgatc taagtacaca
    25261 ggcagttggg tttacactaa gcagcccatt ttatatcaat gcgatgactg gcggaagcga
    25321 aaaaacaaaa aaaatcaacc aagatcttgc aatcgtcgca agagaagcag atttgatgat
    25381 tgccactggt tcagtcagcg ctgccttgaa agtcccttct ttagccgata cttatacgat
    25441 tatgcgacaa gaatatcctc acgggaaaat cattgccaac atcggtgcag ggacttctgt
    25501 cgaaagagcc aaagaagcta tccgattgtt tcacgcagat gccttacaga ttcatttgaa
    25561 tgcacctcaa gagttggtta tgcctgaggg agaccgtgat tttaccaatt ggaaagtact
    25621 tattcaagaa actcaaacag ccattgacgt ccccctcatc gtaaaagaag tcggttttgg
    25681 catgacaaga gaaacgctca atgatctagc tgctttagga gttcatacag ttgatatcag
    25741 cggtcgaagc ggaacaagct tcacccagat cgaaaacgcc cgtcgctcca agcgggaact
    25801 gagttattta gctgactggg ggcaatcgac agtctcttca ttgcttgaag caaatgaagc
    25861 agacacttct atggaaatcc tagcctccgg aggtatccgc aatgcctatg acatatttaa
    25921 agccctttgt ctcggcgcaa atgccgtggg tacttcagga accgtactga cgcatctgat
    25981 gaatcacggc gtagaagaaa ctattatact gatgaagcaa tggcaagaag agcttcgtct
    26041 gctttatact atggtcggcg caacgaatac cgcggcccta caccaacaat ctctgatctt
    26101 ttccggacca gtgaaggatt ggtgcgaagc aagagggatc gacttagtga agtatggaag
    26161 acgcacagaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    26221 gtacaaacgg cggaagtaaa agcaactcct tcggaaataa gctgaaattc acaaaaattt
    26281 gaaaagcaat tttcgtgaat tccttcttat ttcttggagt taaatgcttc tgtcccagcc
    26341 tcttttttcg aataaatgat gtttaatcag ctgtccttcg gtattcgttc aatccgctga
    26401 tttcacaacc tctccactaa acagtgtact aaagctcttt taaggttgtt ttccgatata
    26461 ggcaagtatg cctccgtcca catacaagac atggccgtta acgaaatcag atgcgctaga
    26521 agccaagaag acagatggtc cagccaaatc gattggctcg ccccagcgtg ctgctggcgt
    26581 acgtccaacg ataaattgat cgaatggatg acgttcaccg ttttcttgtg tttcgcgtaa
    26641 tggagcagtt tgcggtgtag caatataacc tggtccaatt ccattacatt ggatattgta
    26701 ttgaccgtat tcagaagcga tgtttttcgt cagcattttt aatccgcctt ttgccgcagc
    26761 ataagcactg accgtttcgc gtcctaattc actcatcatg gagcagatat tgatgatttt
    26821 accgccgcct ttttcgatca tatccggaat aactgctttt gaaacgatga atggggcatt
    26881 taaatccacg tcgattactt ggcggaagtc tgccgcatcc atttcgatca tcggtgtccg
    26941 tttgatgatc cctgcattgt tgactagaat atcaactgaa cctacatctt ctttgatctg
    27001 ttgaaccatc gcttgaaccg cttcttcatc ggtcacatcg cagacataac cttttgcatc
    27061 gatgcctgct tctttataat gcgctatacc ttcatcaact gattcttgat tcaagttatt
    27121 aaatacgatt tttgctcctg ctgtagctaa agagcgtgca atctcaaatc caataccata
    27181 gacagcaccg gtgactaatg ctacttttcc atctaaacga aacatatcca tactaaattc
    27241 catcattttc tctcccttat gcttatttta attgatccat ttgcaccata tccatgtctg
    27301 tataggtgat attttctccg cacattgccc agatgaatga gtaattgctt gtacctacac
    27361 ctgaatggat cgaccagctt ggcgaaataa tggcttgttc attgtctaca actaaatgtt
    27421 ttgtttcatc tggtttgccc atcatgtgga acacacgtgt atcttcattg gcataatcaa
    27481 agtagacata agcttccata cgacgttcat gtgtatgaca aggcatcgta ttccaagcgc
    27541 ttcctggttc taaaatcgta taacccattt gtaattgaca gctttcgcat acatttggat
    27601 ggatgtattg gtagatttta cgttcattca gtgtcaggcc ttcaccagtc tccattggtt
    27661 ttacttggtc gatactgatt tttacatttg gatatttatg atgagctgga acagaactaa
    27721 tatagaattt agctgggttt tcagcatctt gagaagaaaa tacaacatgt cgtgtttctt
    27781 ttccgatata gtatccgtct tgttttttca tttcttcttt ttgcccatcg atctcgatga
    27841 atcctggtcc accgatattg atcacaccta gttcacgacg ctctaagaag taatcaacac
    27901 ctagttcttt gtctaatgtg atttctaatt ctttttcagt aggtgtcact ccaccaaaga
    27961 tcattcgatc gttgtgcgta taagtcaaac tgattttccc tggttcaaat accttttcta
    28021 ctaaaaattc ttttctcaat tgttccgttg aataatgacg gatatcttct ggactgtgtg
    28081 tataacgtgt ttccatttct tgcattttct tctactcctt ctatcgaatg atttttccag
    28141 agccgcatgc taaaaattgt gcgacttctt cttgactaaa ttggttgcag tccccatgga
    28201 tcgtatgctt caaggcagaa gctgccgtag caaaatcaat gatttcttgc ggagacatat
    28261 tggataaaat cccatggagc acgccacctg caaaagcatc tccgccacct actcgatcca
    28321 cgattggggt gattctatgg acttgggatt ccacatattc atggttcatc cacaatgtcc
    28381 ccttcaattc attttcactt gctgaatgaa cggtacgctt ggtcgaataa aacacctgaa
    28441 tattcgggaa cttttcctgc attttttgat aataataaat tagaggttgt tcttctgctt
    28501 ctggcgcttt ctcgatacct aacaaataaa gagcatccat ttttccagct gagcagatat
    28561 ctactaaagg taaaagctga tggattactt cgctggcttc ttgctgtgtc cataattttc
    28621 cacgatagtt gatatcgaag ctgatcaggc agccagcttt ttttgcagct tctatcacct
    28681 gtttcgtcat tttttgccat tttttagaca aagctggggt gattcctgaa acatgaaata
    28741 tatctattcc ttggaataac tcctctagcg accattccaa atgatccatt tcagcaaaac
    28801 ttgatccggc acgatcatag atcactgagg cggcgcgttc gccactacca gtttccatat
    28861 aataagtacc tagtcttgat ccaccagata atacctgttt cgtatctact ccataacatt
    28921 gcaagtgctt tttgactgct tttcccaatg cgttatcagg tacttttgta gcaaaaatcg
    28981 cttcatgtcc gtaattagct aaagaaattg ctacgtttgc ttcccctccg ccataatggg
    29041 cagcgaatcg ttcacttgaa tcgatacggg ttccctgctc tgtggacaat cgcaacatga
    29101 tttctccgag tgtcactact tttcccataa gcagctacct cttgatttct ttcatttttg
    29161 ctgcatactg ttgcgcgact tccgttactt tgtcaaaatc tcctgtagct gcgggtgcta
    29221 aaaggttccc acctactccg actgttacga caccagcatc gaaccactct ttcatattgt
    29281 ccagactcac accgccagtt ggcatgatat tgacttgagg catcggcgcc ttgaaggcag
    29341 aaatgatact tggtccataa gcgcttccag gaaatagttt gacgatatct accccgcttt
    29401 tcagcgctgt tttgatctcg gtaatagtca tacatcctgg taagtaagga atttgataca
    29461 gattgcacat ctctgccgtt tcttgatcaa aggaagggct aacaatatac tctgcgccag
    29521 ctaagatagc caagcgagct gtgacagcgt ctaatactgt gcctgcgcca atcactattt
    29581 ctggttggtc tttgtagaat gacaaaagtt ctttgattac ttgatctgct tgagggactg
    29641 taaaagtcaa ctcaatacca gttagaccac ctttgatgat tgcgtggcaa gcattcaaag
    29701 cttcttcttt gctttttccg cgtaccaccg caatcactcc ggcgtttttc aatcgagata
    29761 aaatatcaat ttttttcatc ttctccacct atttcctatt tagaaatttt attttataac
    29821 ttggaaatct atacccatat tatacggcag aaaagaaaaa ttgcaaacgc ttttttcatt
    29881 atttttgaat tctttgctcg atttcgcttt ttgtttgctt gagggcactg attatttgtt
    29941 cttttgtctc atctgttaaa cgatatttcg gcatacttac actaaatgca ccaatgattt
    30001 gttcgttttt cacgattgag gctcctatac aaaaaatatc acgttcgatt tcttcatcgt
    30061 caaatgcgat gtgggtttct tttaccgttt tgatttcctt tttcaaccgt aatgcattgg
    30121 tgatcgtata ttctgtgtaa gggatcaacg gatgcttttc aagataatca gtgtactgct
    30181 cttcactgaa ttctgctaat acggctttgc ccatcgcaga attgtataat ggtcgggtga
    30241 ttccgatttt tgaagacatg cggatcgttt gatgttttgg atctagtttg ttcacgtaca
    30301 agatctcatt gttgtccaat acccctaagt ggatagtctc atcgatcttt tgctgcagct
    30361 gttctaaaaa cggtaaagtc aactctacca aatcgatttg ttcaatgttt ttattcgcgt
    30421 agcgaataag ctttgcacct aaacgatagt ttttctctga atttttattt acgtagccga
    30481 tcatcaacaa agtatctagt attttcaaga ctgtagaaga ggtcattccc acaccctttg
    30541 cgatcgtctg caaagagaca tcttggtttt ccgctaaata gtctagtatt ttagaggctt
    30601 tgatcaatac agttccataa ggctttttat tttccatata atattctcct atttctttat
    30661 aagaaaaatt atagcggaaa taccatcaat ttaaaagaga tatccagata taaactacca
    30721 tttctcttta caggcaattt aatgttgaag atccgcttcg tttttacatc aaactttgat
    30781 ataaagcaat gatttcttct actgtcgttt ctcttggatt tcctggtgtg catacatctc
    30841 gcaaagcatc ttctgcaatt gctggaatat ctgcttcttt tacacctaat tcagaaatgg
    30901 tttctgggat tccaaccgct ttgctcaacc gatcgatctc accacaagcc gcttctctta
    30961 catcttctaa agacatttca gctgctcctt taatccctag aacaagagcg atctcacggt
    31021 acttttctcc agtatattct ttgttatatt tcatgacagt tggcaaaagt gctgcacacg
    31081 ctacgccatg aggaatgttg taccatgcac ttaacgggtg agccatcccg tgtaccagtc
    31141 ctaaaccgac atttgagaag cccattcccg caatatactg tcctaatgcc attttttctc
    31201 gcccattttg atcgccatct acagaagctt ccaaagaatg gccgatgatt tcgattgctt
    31261 ttaaatgcag catatcagtc atttcccaag cgccttttgt gataaaccct tcaatagcat
    31321 gcgtcatagc atccatacct gttgatgcag ccaatttttt cggcattccc atcatcatat
    31381 cgctgtcgat gaacgcgacg attgggatat catgaggatc aacacaaaca aattttcggt
    31441 gatttacttc atctgtgatg acgtagttga tcgttacttc tgctgctgtt ccagaagtcg
    31501 ttggtatagc taatataggt aaacatggat ttttcgtatc agcaacacct tctaaactaa
    31561 tgacgtctga aaactctgga ttcgtcaaaa taatcccaat ggcttttgcc gtatcgattg
    31621 gcgaacctcc accgattgcg atcaaacagt ctgcctctgc ctctttaaca aaagccactc
    31681 catcttttac attttggatc gttggattag gtacgatccc gtcgtataaa gcataagcga
    31741 ttgctgcttt atctagaagt tctgttactt ttgttgctat accgtgctcg atcaatcctt
    31801 tgtcagtaat gacagctgct ttgccgaatc ctcgctgctg aaattcaggg acgatcgact
    31861 caatcgctcc ttttccaaaa tacgatgttt catttaaaat cattcgatta ctcattttct
    31921 attcctccta atttgtatta tttttcacaa taaaaacgaa agaaccccaa gcaaccggtt
    31981 gcccagttgc ttgttgctac agttcaaaga ctggcatcaa ttcccaactg actggagagg
    32041 catctggatt tgtctccatg atatcttcca tgtacttcca ccatttttga ttgatagatg
    32101 tatttgccat tttttcccat aacttttcat cttcgatcac taagtaacca aataacaaac
    32161 ttgtactcgg atcaagatag atcgaatagt cgatgacccc gtgttcacga agtgctgtac
    32221 gcatttctgg ccaaagttca tcatgtcttt ttttgtattc ttcttgtttt tctgggtaaa
    32281 ctttcatgca aaaagctttt ttcaccatgt cagacacctg ctttcgtatg aacttcctcc
    32341 aaataacctg cttttggagt gacaccaaac gcatctgcta aatgccacag gttttcgtcc
    32401 gtgattgttt gcaagattgg tccttgcgct ttgacgtacg tatagacttc tgctgccttt
    32461 tctgctgtct cgatcaaacc aaagacttca tccatgtctt ttcctgaccc gtagattcca
    32521 tgttgtggcc ataaaaccaa tcgagtttct ttcatttttt cagctgttgc ttccccaatc
    32581 tcatttgttc ccggaaccat ccacggaatg atggaaattc cttctggaaa aacaactaag
    32641 cattccgtac acatttgcca tagcgtacga ctaaaggaac gttcgtctaa ttcatgcgta
    32701 aaactcatcg ctaatagatg actagcatgg caatgcataa cgatccgatt atctgggtct
    32761 acttctaaac gggcgatatg actcatgaaa tgacttggca actcgcttgt aggaaggcat
    32821 tcgttttcca gcccccacaa aagttccagt gttttgccat cttcagcaac tcgaacgatt
    32881 ccaagattct cggcaggttc gctcgctaca tttttaaaat atcttcctga accagttaca
    32941 ataaaatagc atcctgctaa acggctggca tcaaaaatca tcgagatgcg gcggatcact
    33001 ttttcaggat ccaagaacgg tgtgatctct tcttctttta gtaaataaga aatattcccg
    33061 ccatttcttt catcccatcc caagcgataa agattcgacg tcgtttcgat catttctttc
    33121 acataaggcg cttgtaaaat atctattttt ttcaatatag ccacactcct attttcttaa
    33181 ttccaacacg tctttttgat attcctggac ttcttccagc caatccaaac caactggaac
    33241 ttgatttttg taacagaaat aattccatac atcggcatac gggaaatctt tcaattcttc
    33301 cgtataagcc attcttgaag taaagtcaaa agccagctcc atttctttca atcgctcaac
    33361 tggttccagc atagctttca gcagtgcttt ttgtgtattt ctcgtaccga tgacccaagc
    33421 agcgatacgg ttgatcgttg catcaaagaa atccagaccg atatttgttt tttccaacaa
    33481 ttcgttacgg actaattctt tagcgatttc ctgcaattca tcatccatca aaaccacatg
    33541 gtctgaatcc caacgcactg gacgggaaac atgcaacatg attcctttgc tgaataaagc
    33601 taatgaagat aatttattgg aaatcacttc tgttggatga aaatgtccag catctagaca
    33661 aacaagtttg tcacgagtca acccgtaacc catataaaat tcatgggaac cgactgtata
    33721 agcttcagcg cctaaaccaa atagtttgct ttctactgca tcttgtacat accgttcatc
    33781 taactcttct gtaaaaattt catcgagtga ttccattaaa cgttcccgtg gggtaaaacg
    33841 atcaatcgga ttatctttca tcccatcagg tacccaaaag ttattgatac ttgtttgccc
    33901 agtttctttc ccaaagtact ccgcgatttt gcggcttctt ttcccgtgtt cgatccagta
    33961 ttcacgaact tctttatctg gatgagccaa tgtaaagccg tctttcatca ttggatgcga
    34021 aaagaaagtc ggattgaagt caagaccgat ttcttctttt tttgcccact ctacccattt
    34081 ttcaaaatgt ttcggctcaa tttcatttag atcgacagat tcttctgtat ccaaatagat
    34141 agcatgaagg ttcaatttgt gtttgcccgg tatcaacgaa taagccttct ccagatcttg
    34201 tctaagctct tcaggtgttc gtgcggctcc tggaaaattc cctgtcgcca tgattccacc
    34261 ggtcaattcg ccatccggtg tcaagaaacc tttgacatca tctccttgcc agcaatgcat
    34321 agaaattttg atgtcctgta tcttttctag tacagcatct gtatctacac cgatagttgc
    34381 atatttttct tttgcttgtt cgtaatttct ttcaatcgtg ttcatacgat tactcctttg
    34441 attttattca cgtaccattc catcgtgttt tgcttatttt catctgggta aaaagtttca
    34501 catggaaacg actgtttgat tacatttctt gcttgttcta tattcgaaaa ctgacctgta
    34561 ctgagcattt gcatggcaac attcccgatt gctgtggctt cactaggtcc tgcttctaca
    34621 atgattccgg cataatctgc tgtcaattga ttcagtagac gattattcga tccgccccct
    34681 acgatgtgca gcttttccaa tttcgttcca gttaagtgtt ctactttgat cagctcttgt
    34741 gcgtaacaaa gagcaagatt atcatacaca caacgagcga tagctcccga tgagacaggg
    34801 actggctgct ctgtttcaaa acagtattgc tgaatctctt ctatcatatt tgggggattc
    34861 aaaaatcgag ggtcattgat ttccatcact tgctgcagcg gtttttcttt tgcagcaagc
    34921 tctgccaatt ccgcataact gtactcgtac tcttgcattc gggcaacttc ctggatcaac
    34981 cacatcccca tgatgttttt caaaaaacgc accgtgttat gggcacccca ttcattcgta
    35041 taattttcag aaaaagcatt gcgagaaaca ttggcgatct ttgtttcaat ccctaaaagc
    35101 gaccatgtcc cactgctgat ataaccccaa cgttctcctt gacctggcgt tcctaataca
    35161 gctgacgccg tatcatgact tgctactgtg ataaaggtca catctggcaa atcatatttt
    35221 gaaaattgtt ctttttgcag atctcctagt atcgttcccg gctctgtcaa tggtggaaat
    35281 aattccttcg agacaccgat tttttcaaga agttctattt cccattcttt tgtccctgca
    35341 ttcagcagct gcatagtaga tgcattagtt ttctccgtca ccatgttacc agtaaagaca
    35401 taccctaaat aatcaggtat cagtaacaat ttatgtgctt ttgccaaatt ttctttttct
    35461 tcagcaaaca attggaacaa ggtattgaaa ggctggatct gaatccctgt ctgctcatac
    35521 aacttatcta gagaatagat ttcagaaaac tgttctactg ctccattggt acgcttgtcc
    35581 cgataagcaa tcggatcatt tatccgatcc ccttgttcat caatcagaca gtaatcgact
    35641 gcccatgtat cgatccccgc aaaaaggcga tcaatccctg tctgctttaa tttttcaaat
    35701 ccaatcaaaa tctctttgat aagataatcg atcttccagc gatcgtgacc gtctttgttc
    35761 cgaaagccgt ttttgaaccg gtgtacttct tccaagatca aagaaccttt tgcatccctc
    35821 gtacttttca ttagtcgtcc gcttgatgca ccgatatcga tggcaacaaa tgttggattc
    35881 atttctccac ctcttttact ttattgttga ttagcacttt attaataaag cgttatcatt
    35941 tattgacaat gctatcataa tacagaatct tatattttgc ttcacactat tttggtatat
    36001 aattgcatta tcttgacaag ttgggagtgt ttacatggat tactgggaaa aagccaacca
    36061 ctcatggtcg gaagactcaa aaagagtgat cttgacgcct acacaaagaa gcaggaattt
    36121 atttttttat atccaagaaa tcggttactt caaagcacca atcccttact atactgaacg
    36181 agcacattta ccttcttact tgataaagtt tactttgtct ggcaaaggtt cgcttacata
    36241 taatgataat cagcaacagc taaaagctgg agacttattt tttattgact gccaagaata
    36301 tcagcgttat caaacgatca gcgaagaacc atgggaaatg gattggatcc atttgaatgg
    36361 tcagaacgtc caatcatttt atcaggagtt cacaaaagac ggccataaca ccttccattc
    36421 aacaggagat ccatcagaaa acaagatcca ccatttgatc acagaattga tccacatgca
    36481 acaaggcacg aatgcccgta ccgatttcca gtcctctgta gctatccacg agttgctgaa
    36541 tgaactgatt ttgcaaaagt atcagctaga tttcgaagaa gaggatattc ccgcctacgt
    36601 ttacaaactg aagcaatact tagacgaaca tttcaaagaa acgattacac ttgatcaatt
    36661 agaacaacaa ttcttattga ataaatatca gctgagcaaa gatttttcca aatttatcgg
    36721 ttctccacca atcgattatt tgatcagcaa aaaactttct tacgctaaag accttcttcg
    36781 gtatacagaa tttacggttc gaatgatttc cgaagaaata gggattgaga attttgctta
    36841 ctttagccgc ctgttcaaag caaaaacagg actatcgccg agagactttc gtaaattcag
//
LOCUS       DAFCHQ010000017        35882 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000017 DAFCHQ010000000
VERSION     DAFCHQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.017"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1152
                     /locus_tag="LNC71_000963"
     CDS             <1..1152
                     /locus_tag="LNC71_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBK6466052.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="LNC71_000964"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="LNC71_000964"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBK6466053.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="LNC71_000965"
     CDS             2728..3003
                     /locus_tag="LNC71_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBK6466054.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="LNC71_000966"
     CDS             3009..3776
                     /locus_tag="LNC71_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBK6466055.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="LNC71_000967"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="LNC71_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBK6466056.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="LNC71_000968"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="LNC71_000968"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBK6466057.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="LNC71_000969"
     CDS             7920..8222
                     /locus_tag="LNC71_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBK6466058.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="LNC71_000970"
     CDS             8225..9388
                     /locus_tag="LNC71_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6466059.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="LNC71_000971"
     CDS             9414..12842
                     /locus_tag="LNC71_000971"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBK6466060.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="LNC71_000972"
     CDS             12863..13987
                     /locus_tag="LNC71_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466061.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="LNC71_000973"
     CDS             13977..14414
                     /locus_tag="LNC71_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6466062.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="LNC71_000974"
     CDS             14398..14736
                     /locus_tag="LNC71_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBK6466063.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="LNC71_000975"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="LNC71_000975"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBK6466064.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="LNC71_000976"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="LNC71_000976"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBK6466065.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="LNC71_000977"
     CDS             15893..16936
                     /locus_tag="LNC71_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBK6466066.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="LNC71_000978"
     CDS             17095..17823
                     /locus_tag="LNC71_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBK6466067.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="LNC71_000979"
     CDS             17939..18184
                     /locus_tag="LNC71_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6466068.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="LNC71_000980"
     CDS             18181..18399
                     /locus_tag="LNC71_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBK6466069.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="LNC71_000981"
     CDS             18535..19074
                     /locus_tag="LNC71_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBK6466070.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="LNC71_000982"
     CDS             19067..21340
                     /locus_tag="LNC71_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBK6466071.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="LNC71_000983"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="LNC71_000983"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBK6466072.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="LNC71_000984"
     CDS             complement(22513..22767)
                     /locus_tag="LNC71_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBK6466073.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="LNC71_000985"
     CDS             complement(23167..23436)
                     /locus_tag="LNC71_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466074.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="LNC71_000986"
     CDS             complement(23906..24361)
                     /locus_tag="LNC71_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBK6466075.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="LNC71_000987"
     CDS             complement(24499..26427)
                     /locus_tag="LNC71_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK6466076.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="LNC71_000988"
     CDS             complement(26624..27457)
                     /locus_tag="LNC71_000988"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBK6466077.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="LNC71_000989"
     CDS             complement(27649..28353)
                     /locus_tag="LNC71_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6466078.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="LNC71_000990"
     CDS             complement(28376..29002)
                     /locus_tag="LNC71_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6466079.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="LNC71_000991"
     CDS             29328..31703
                     /locus_tag="LNC71_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBK6466080.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="LNC71_000992"
     CDS             complement(31727..32218)
                     /locus_tag="LNC71_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBK6466081.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="LNC71_000993"
     CDS             32405..33325
                     /locus_tag="LNC71_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6466082.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="LNC71_000994"
     CDS             33479..33823
                     /locus_tag="LNC71_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBK6466083.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="LNC71_000995"
     CDS             complement(34013..34513)
                     /locus_tag="LNC71_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466084.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="LNC71_000996"
     tRNA            35041..35124
                     /locus_tag="LNC71_000996"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="LNC71_000997"
     CDS             complement(35448..>35882)
                     /locus_tag="LNC71_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBK6466085.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7404 g        10348 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DAFCHQ010000018        35033 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000018 DAFCHQ010000000
VERSION     DAFCHQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.018"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..196)
                     /locus_tag="LNC71_000998"
     rRNA            complement(<1..196)
                     /locus_tag="LNC71_000998"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            937..1626
                     /locus_tag="LNC71_000999"
     CDS             937..1626
                     /locus_tag="LNC71_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6466086.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1613..2821
                     /locus_tag="LNC71_001000"
     CDS             1613..2821
                     /locus_tag="LNC71_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6466087.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            3115..4386
                     /gene="serS"
                     /locus_tag="LNC71_001001"
     CDS             3115..4386
                     /gene="serS"
                     /locus_tag="LNC71_001001"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBK6466088.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4935..6419)
                     /gene="guaB"
                     /locus_tag="LNC71_001002"
     CDS             complement(4935..6419)
                     /gene="guaB"
                     /locus_tag="LNC71_001002"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBK6466089.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6924..7619)
                     /locus_tag="LNC71_001003"
     CDS             complement(6924..7619)
                     /locus_tag="LNC71_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBK6466090.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7709..8809)
                     /gene="ychF"
                     /locus_tag="LNC71_001004"
     CDS             complement(7709..8809)
                     /gene="ychF"
                     /locus_tag="LNC71_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBK6466091.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8818..9009)
                     /locus_tag="LNC71_001005"
     CDS             complement(8818..9009)
                     /locus_tag="LNC71_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBK6466092.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(9030..9920)
                     /locus_tag="LNC71_001006"
     CDS             complement(9030..9920)
                     /locus_tag="LNC71_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBK6466093.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9907..10674)
                     /locus_tag="LNC71_001007"
     CDS             complement(9907..10674)
                     /locus_tag="LNC71_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6466094.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10686..11399)
                     /gene="rsmG"
                     /locus_tag="LNC71_001008"
     CDS             complement(10686..11399)
                     /gene="rsmG"
                     /locus_tag="LNC71_001008"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBK6466095.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11631..13532)
                     /gene="mnmG"
                     /locus_tag="LNC71_001009"
     CDS             complement(11631..13532)
                     /gene="mnmG"
                     /locus_tag="LNC71_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBK6466096.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13549..14946)
                     /gene="mnmE"
                     /locus_tag="LNC71_001010"
     CDS             complement(13549..14946)
                     /gene="mnmE"
                     /locus_tag="LNC71_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBK6466097.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15245..15628
                     /locus_tag="LNC71_001011"
     CDS             15245..15628
                     /locus_tag="LNC71_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466098.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15796..16554)
                     /locus_tag="LNC71_001012"
     CDS             complement(15796..16554)
                     /locus_tag="LNC71_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBK6466099.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16572..17390)
                     /locus_tag="LNC71_001013"
     CDS             complement(16572..17390)
                     /locus_tag="LNC71_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK6466100.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17387..17743)
                     /gene="rnpA"
                     /locus_tag="LNC71_001014"
     CDS             complement(17387..17743)
                     /gene="rnpA"
                     /locus_tag="LNC71_001014"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBK6466101.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17861..17995)
                     /gene="rpmH"
                     /locus_tag="LNC71_001015"
     CDS             complement(17861..17995)
                     /gene="rpmH"
                     /locus_tag="LNC71_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBK6466102.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18518..19852
                     /gene="dnaA"
                     /locus_tag="LNC71_001016"
     CDS             18518..19852
                     /gene="dnaA"
                     /locus_tag="LNC71_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBK6466103.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            20050..21180
                     /gene="dnaN"
                     /locus_tag="LNC71_001017"
     CDS             20050..21180
                     /gene="dnaN"
                     /locus_tag="LNC71_001017"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBK6466104.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21409..21651
                     /gene="yaaA"
                     /locus_tag="LNC71_001018"
     CDS             21409..21651
                     /gene="yaaA"
                     /locus_tag="LNC71_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBK6466105.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21638..22762
                     /gene="recF"
                     /locus_tag="LNC71_001019"
     CDS             21638..22762
                     /gene="recF"
                     /locus_tag="LNC71_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBK6466106.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22759..24705
                     /gene="gyrB"
                     /locus_tag="LNC71_001020"
     CDS             22759..24705
                     /gene="gyrB"
                     /locus_tag="LNC71_001020"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBK6466107.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24725..27196
                     /gene="gyrA"
                     /locus_tag="LNC71_001021"
     CDS             24725..27196
                     /gene="gyrA"
                     /locus_tag="LNC71_001021"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBK6466108.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27432..27731
                     /locus_tag="LNC71_001022"
     CDS             27432..27731
                     /locus_tag="LNC71_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBK6466109.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27781..28293
                     /gene="ssb"
                     /locus_tag="LNC71_001023"
     CDS             27781..28293
                     /gene="ssb"
                     /locus_tag="LNC71_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBK6466110.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28319..28555
                     /locus_tag="LNC71_001024"
     CDS             28319..28555
                     /locus_tag="LNC71_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBK6466111.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28698..30671
                     /locus_tag="LNC71_001025"
     CDS             28698..30671
                     /locus_tag="LNC71_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBK6466112.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30677..31129
                     /gene="rplI"
                     /locus_tag="LNC71_001026"
     CDS             30677..31129
                     /gene="rplI"
                     /locus_tag="LNC71_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBK6466113.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31403..32770
                     /gene="dnaB"
                     /locus_tag="LNC71_001027"
     CDS             31403..32770
                     /gene="dnaB"
                     /locus_tag="LNC71_001027"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBK6466114.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33550..34842
                     /locus_tag="LNC71_001028"
     CDS             33550..34842
                     /locus_tag="LNC71_001028"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBK6466115.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10648 a         6823 c         6929 g        10633 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttgtt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcaggag tgtttcccga
      361 ttcgtttttg gctgccgtag caacttttat atcatatcag agagacaagt aattgtcaac
      421 tcttttttaa aagttttttc gatttcgttt ttcgaaatct tccagcaact catttatcat
      481 aacttatcgt ttgttatttg tcaagaactt ttttgaagtt tttttcgata gaatcaagct
      541 tcgcaagtgg ttctttttta acgactcagt tatcttatca tgtcatttcc aacatgtcaa
      601 caacttattt tttattattt aataagttat tggtgttctt ttgaacaaat aataatatat
      661 caacatcaca tgtgaaagtc aataagaaaa tgcttattta tcaacatttc tagcttacat
      721 gatcattggt tagttctggt cctttttcgt tcgtctttat aactccgaga gacgtaaaat
      781 ccttttgttt tcagtttaat gttcatgttc ttgtcttatt cacaccaact agatgtctca
      841 ccttctaaaa ttttccttaa caaagtgctg cttttatcta tttctgcttt tttttgctag
      901 aataatagta acaatatgac tctggaggat ttatttatga aaattttgat tgctgacgat
      961 gataaagaaa tcgttgaatt attaagtatc tatgttcaca atgaggggta tgaagcagta
     1021 aaggcttatg atgggaaaga agcattatct aaaatcagaa caatccctga tattgaatta
     1081 cttattttag atatcatgat gcctgagatg gatgggatgc aagtagtgaa agagctacga
     1141 aaagaatccc aaataccaat cattatgctg acagcaaaaa ctactgatat ggataagatc
     1201 aaaggactgg tcgctggtgc agatgattat gtgaccaagc catttaatcc gttagaagta
     1261 atggctagag taaagtcttt attacggcgt acaaaaatgc agattgcccc agatcagcct
     1321 gatgtattag aagtaaatgc attaatcatc aataaagact ctcatgaagt taaaacaatc
     1381 gatgggaaag aaatccagtt aacagctttg gaattcggta tcctctactt gcttgctagt
     1441 catcctaatc gagtatttag cgcggatgaa atttttgagc gtgtttggaa acaagaaagt
     1501 attgtctctg ctaaaactgt catggttcac gttagtcatt taagagataa gattgaagaa
     1561 gcaactggtg gtgaaaaagt cattcaaacc gtttggggag tgggctataa aattgacgca
     1621 agatagtaaa cgagcaactg aaaaacgccg tcggatcaat ttgacttcaa aagaaatcag
     1681 cgaattgtta gcggaaggaa tcatcacgat tatccttttg cttctattaa atgtttctat
     1741 tctggttgtt atcagttcgg tgatcaacag ttcaccttct ttgacgaatg cgatttggga
     1801 ttcaaagaat atttttgcag aacgattgaa cactgatttg ttctggaacg gccggaattt
     1861 tattattccc tttttctttc tattagatat tggtgttttg tattggcgct tgattcgaag
     1921 atatcgccaa atgcaattgc ggcatatcat cagcgaactt cactacatcg caaacggaaa
     1981 ttatgatcat cgtatccctt ttgaactgag tggtgactta agccgagtgg taacaagtat
     2041 caatggattg gtggatagta ccgtagcagc gattgaagac gaacgtaaaa tcgaaaaatc
     2101 aaaagatgaa ttgatcacga atgtcagtca tgatatccgt accccactca cgtccatcat
     2161 tggataccta ggactgatag aagatggcca gtatcactca gaagaagatc ttttaaagta
     2221 tacgcatact gcttatatca aagcaaagca gatgaaatct ttagtggacg acttatttga
     2281 atacactaag gtacgccagc ctgctgtccc ggtgaatttt tctgcctttg acatgatcca
     2341 gttgatcgaa cagttagcag ctgattttga gttggaagct tctaaaaaaa atatccaaat
     2401 acttgttcaa tcaaaagtag actcccttat catggatggc gataccgaaa aacttgtacg
     2461 tgtcttcaat aatcttttga cgaatgcgtt gaaatacgga aaaggagcaa cgaagatcgt
     2521 aatcgaagta gaacgtatcg gttcagaagt tgttgcaaca gtcaaaaata acggagcaat
     2581 gatcccacaa caggcaatcg ataatctatt cgaccgtttt tatcgtgtag aagaatctcg
     2641 ttcacaagca actggaggaa ctggacttgg tttagcaatc gctcaaagca ttgtggcttt
     2701 gcacggcgga tacatttatg caaaatcaga taagcaatgg acttctttca tcatgcacct
     2761 tccaatcaag aaaaatgaaa aattacctat tgatacacag gaagttattg acgaaagctg
     2821 aaatattcgc tattcttatt acaattatta gaataaatga aaaacacaga acccagtaca
     2881 ctttattctt tttcagagag ctgatggcag gtgtaaatca gcaaaggata atttggaagt
     2941 tccagttcgt ttacgtgccc tttcgaaata agaaaggtcg gttcaacagc cgttatgtgt
     3001 tcaacaagtg cagctgacgc tgaacttggg tggtaccgcg aatttcagac tgattcgtcc
     3061 caagaataat gggatggatc agtctttttt tattttcaaa aaggaggaaa tcatatgtta
     3121 gatgtaaaaa tgatgcgaca aaatattgaa gaagtacaag aaaaattgat gactcggggc
     3181 gtaaaaaaag aaacattagc tgaattcatg gaactagatg aaaatcgtcg taagctacta
     3241 gtcaaaagtg aagaattaaa aaaatatcga aatgatgttt ctgctgagat cgcccaattg
     3301 aagcgtgcaa aagaagatgc ttctgctaaa atcgctgaaa tgaaagaagt cggtggcaac
     3361 atcaaagcat tggatactga aattgctgaa gtggatgaaa aattgaaaca aattgcaact
     3421 actttgccaa accttcccca tgattctgtg ccagttggaa cagatgaaga ggacaatgta
     3481 gaaatccgtc gttggagcga accacgtaca tttgcttttg aaccaaaacc acactgggaa
     3541 attgcagaaa atcttgatat tttagatttt gaacgtggcg caaaagtagc cggtagccgt
     3601 tttgtctatt acaaaggatt aggtgctcga ttagaacgtg ctgtctacaa cttcatgctt
     3661 gatcagcata tttacgaaca cggctatact gaaatgatca ctccttatat cgtcaacagt
     3721 aaagcgatgt ttggtacggg acaatttcca aaattcaaag aagatgtttt ccaattggag
     3781 aatactgatc taacattaat cccaacagct gaagtaccat taactaacta ttataatgat
     3841 gaaattttag atggtgcaga tttacctatt tatttcactg cactaagccc tgcttttcgt
     3901 tctgaagccg gaagtgctgg tcgggataca cgaggactga ttcgtcttca ccaattcaat
     3961 aaagtagaaa tggtcaaatt ttcggatgta gagcattctt acgaagaatt ggaaaaaatg
     4021 acagctgatg cagaagatat cttgaaaaaa ttgcatttgc cttatcgtgt gatggcctta
     4081 tcaactggag acatgggctt ttctgcagct aaaacttatg acttagaagt atggatacct
     4141 gcacaagata cttatcgtga aatcagttct tgttctaact gtgaagattt ccaagcacgc
     4201 cgcgcaatga tccgttatcg cgataatgat ggaaaaatcc actatactca tactctgaat
     4261 ggttcaggac ttgcagttgg ccgtacagta gctgccattt tggaaaacta ccaaaatgaa
     4321 gatggttctg tcacagttcc agaagcgctc gttccttata tggggaatct gaaagttatt
     4381 aaataaagag gctgtgaaaa agccgcttag tttcgagtat taagaaaaat tttgtgaaaa
     4441 tggctttcca catttttgca taaattggac ttaataccga gaaactggct tttgaacacc
     4501 atttatcgag aggttgtgaa atcagcgaat taacctgagt attagagaaa aaaatggaaa
     4561 aatagtccct catattttac cgtttgttga actaataccg agggtcagct gattgaacac
     4621 cgtttagtgg agaggtcgta agcctacccc ccagctccta gttacaagga caaattcaaa
     4681 aaacagctct tcatatttta tgaatgttgt acttgtgatt cggaggtggc aagggaacac
     4741 tgtttattca aaaagaggct gggacagaag cgtttaactc caagaaataa gaagaaattc
     4801 acgaaaattg cttttcaaat ttttgtgaat ttcttcttat ttccgaagga gttgcttcta
     4861 cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac
     4921 gctctttaat gtttttaaga ttcaactgaa tagtttggtg cttcttttgt gatttgtaca
     4981 tcatgagggt gtgattcttt taatccactg ccgctcattt ggataaattg cgcttcatca
     5041 cgtaattgct gtagattggc tgccccaaca taacccattc ctgaacgcaa accgccgatc
     5101 atttggaaaa taatatctgc aacacttcct ttataagcaa cccgaccttc gattccttct
     5161 ggtactaatt tatttgcttc attgacacta ccttggaagt aacggtcttt tgaacctttt
     5221 tccatcgcgc ctaatgagcc catacctcgg tatgttttga atcgacgtcc ttggaagatt
     5281 tcgaattcac ctggtgattc atctgtccct gccaacatac ttcctagcat cactgcatgt
     5341 ccgccggcag ctaatgcttt gacgatgtca ccagaatatt tgatccctcc gtctgcaata
     5401 atcgctttgc catattgacg agcaactgat gctgcatcat aaatagctgt gagctgaggt
     5461 acacctacac cggctaccac acgtgtcgta caaatcgatc ctggtccgat accaactttt
     5521 acgacatcta cacctgcatc atataaagct tttgtcgctt cagccgtcgc aacgtttcct
     5581 gcaatcaatg ttgcatctgc aaaagttgaa cggatttctt gaatttttcg gatcacacct
     5641 gcactatgtc catgagctgt atcgatgatg attgcatctg cccctgcttc taataaagca
     5701 ttcgcacgct caaatgtatc gcttgttaca ccaacggctg ctgctacaag caatcgacca
     5761 tgttcatctt ttgctgcatt tggaaattcg atcactttct cgatgtcttt gatcgtgatc
     5821 aatccgctta aacgaccttc gttatctacg attggtaatt tttcgatttt atgtttttgc
     5881 aaaatctttt ctgcatcttt tagagaggta cctactgggg cagtcactaa gttgtctttt
     5941 gtcatcacat cagcaatcgg catgtgataa tctgttacaa aacgcatgtc gcggtttgta
     6001 attatcccta ccaattttct gttttcaagt gtttcaacga ttggcacacc actgatacga
     6061 tatttgctca tcaattcttc tgcgtctgct acaagatgct gtggcgttaa gaagaacgga
     6121 tcaatgatta cgccactttc agaacgtttg acttttctta cttcatccgc ttgctggctg
     6181 atcgtcatgt ttttatggat cacacctagt ccgccttgtc ttgccatagc aatggccatc
     6241 ttgctgtcgg taacagtatc catacttgca ctgatgattg gaatattcaa cttaatattt
     6301 ttagctaatt gtacgctcat atccacttcg tttggtaaca cgtgactttc tgctggtatc
     6361 aataagacat catcaaaagt aaatcctttt ttcgtgaact ttgtttccca gttggacatt
     6421 tttagtacca ctcctctaat ttagtttatc tattaaaata attgattttt tcatttaagt
     6481 caatgatcta cttggaatca agttagcttt ctcatagaat accaatataa agcgtttaca
     6541 tctgtgtgta ttaaaagaga ttgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc
     6601 aatcaaaaac agcttctcat gtttttgaat tggttgggtt tattaccgca gatgcaaact
     6661 tttgaacacc gtttagtgga gaggctgtga aaaagctgct tagtttcgag tagtaagaaa
     6721 aattttgtga aaatggcttt ccacattttt gcataaattg gacttaatac cgagaaactg
     6781 gcttttgaac accgtttatc gtaaaaaggg actgtgacaa gacttttgtc acagtccctt
     6841 actattattt aactatggat aaacaaaatg aggttcttta ctacccctag atatctagca
     6901 aaatagctga tctttttgct agtttatctt cttgggacgg ataaacttcc aataatcccg
     6961 tattttttct tcaagtagta acggattgct aaagcagttg ccccgataat gataactact
     7021 actggatcta atactggatt gataactggt ggcaatagtg ttgtcgcaga aaacagtacg
     7081 acccaaagaa gtgtcgttcc acctaggata agcaatgtct tacccattcc tggtttcttg
     7141 cttcgatctg ctccgggata ttcatattga tagatgtatt tgtacatcat ataaaagacc
     7201 cagccaccga ttgcagaacc tagaacgagt gtagaaaagc cataagctgg cgttgttcca
     7261 cgtgaaaaca atccggtaat cgcaatcatg atacttaata gaccaagcaa cagcaacgta
     7321 ttatctaacc acatcaataa aggggtggat gaatttttta cttctgctgg cttattgatg
     7381 attgcttccg ttctttcaga gactgtccca aacaattgac gggctgttct tccgcctttt
     7441 tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt ttactttttc ttcttcagac
     7501 aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt tcttttggtt
     7561 aattttgttt ctaaactatt gttttctttt acgattgctc gtaattgttc tggttccatt
     7621 atctaatagt cctccttagc cgttcctttt ctcaacggtc gtcacaaaag acattctacc
     7681 atattctaca gctagtttga tagccctttt acacattaaa gcggaataac attacgtcgc
     7741 cgtcttgtac gacataatct tttccttcta atcgaacacg tccagcttct tttgctgcat
     7801 gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg ataaatccgc
     7861 gttcgaaatc tgaatggatg attcctgctg cttgtggtgc tttgattcct tttttgaatg
     7921 tccaagcacg tacttcttgt tccccagcag taaagtaagt agctaagcct aacaaatcat
     7981 aagcagcacg gatcaactga tccaaacctg attcttcgat tcctaaagct tctaaaaatt
     8041 ccgctttgtc ttcttcatct aattctgcga tttcttcttc tgcacgagca ctgaccacga
     8101 tcacttctgc attttcttct gcagcaaagt tgcgcacttc ttgtacataa tgattcgcgt
     8161 ctgcatctgc tacgtcatct tctgagacat tggctacata aagaattggt tttgtcgtca
     8221 ataaaaatag tgatttaacg attttttgtt cttcttcagt aaattcgatc gttcgggcag
     8281 attttccttc ttccagtaca ggtttgattt tatccagtac agctagttct gccactgcat
     8341 ctttatcttt ggttttagca acttttgcta cacgagtata gcgtttgttg atagattcta
     8401 agtctgctaa cactagttcc aagttgatcg tatcaatatc ttctagtgga tcaacacgtc
     8461 cttctacgtg agtaatatta tcatcatcaa aacagcgaac aacgtgacaa atagcgtcta
     8521 cttggcggat atggcttaag aattgatttc ccaaaccttc tcctttactt gcgcctttta
     8581 cgattccagc aatatctgta aattcaaaag tcgtcggaac agtttttttc ggatgtacaa
     8641 gttctgttaa gcgttgcaaa cgccagtcag gtacttctac catccctacg ttggggtcga
     8701 tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt tgtgatcgca ttaaacaaag
     8761 ttgattttcc tacgttgggc aacccaacaa ttccagcagt caatgccata aatttattca
     8821 ctctttctct gcattttttt caattacttt ttttaatttc ttttcaaaat ctcgacgggt
     8881 catcatgacg atatgtccgc aattcataca tctgattttg atatctgcgc ccattcgaat
     8941 gacttcccag cgattcgtct ggcaagcgtg aggttttttc atttcaacga tatcgtgcaa
     9001 gtcgtacata aaaatagcct cctttttgtt tattcttcat caaattgaat attcaagata
     9061 tcaagaattc gagtcaaatc tttttgagaa agatattcaa tctcgatctt acctttccca
     9121 tttttttctt ggatagctac acttgtacca aatttatcca tcaaacgatc ttcgctttct
     9181 cggatataat atggtttttc ctttgctagc cttgggattt ttttcttttc ttttccatca
     9241 ttttgattta actcagaaac aagctgttcc agttggcgga cagttaaatt ttcagttact
     9301 gcacgattgg ctaatttcaa gatcaatgtt ttatttttta aacctaacag cgtacgggct
     9361 tgtcccatag ataaacgctg atcttgtacc atttccttga caagatccgg taaagaaagc
     9421 aagcgcaaat aattggcgat ataaggtctg ctctttccta aacgactagc tacttctgct
     9481 tgtgttagtt tcaggttttt catcagcatc tcatatgctt ctgcttcttc taacggattc
     9541 agatcttctc gctgcaagtt ttccagcact gctacttgca tcatcgcttc ttcgtcaaag
     9601 tctctaacga tagcagggat cgtttctttt tcagctagtt tggacgctcg aaaacgtcgc
     9661 tcccctgcaa taatctcata tccttttact gcggaacggc gtacgatgat gggttgaaag
     9721 acacctgatt gttcaataga attagctagt tcctgcaaag atacttcttc aaatgtttta
     9781 cggggctgat acggatttgg tcgcagctca ttcaatggta gctggacaac ggtatcatct
     9841 tttacatcca ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat tcctcggcct
     9901 aatcctttac ttttcttcac gacttaccac ttcctttgcc aatgcttggt agacttcagc
     9961 tcctcgagaa cgtggatcgt agtcgatgat cggttttcca tgactaggtg cttctgataa
    10021 acgaatattt cttggaatga tcgtttcgta aactttttcg cggaagtatt tacgtacttc
    10081 ttctacgact tcatttccca gatttgttcg tgcatcatac attgtcagta agactccctc
    10141 gatctctaat tctggattaa agtgtttttg gactaaacga accgtattca gtaattggct
    10201 taatccttcc aatgcgtaat actcacactg aacaggtatc aaaatcgaat cacttgctgt
    10261 aaatgaattg attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa tataatcata
    10321 ctgactgctt acttctgcta aagatccttt taatcttgat tctcttgcca tcattgacgt
    10381 caattcgatc tctgctcctg ctaattggag agtcgcaggc acaatactca aattttcgtg
    10441 ttcagtgatc aaagttgcct catcaattgg caattcattg actagaacat catagatgtc
    10501 tcttgtcaca tcaggttttc tgatcccgac accactggta gcatttcctt gtgcatccat
    10561 atccactagc aatacttttt tgccaaggct agcaagacaa gcacctagat tcaccgtcgt
    10621 cgttgttttg ccgacgccac ctttttggtt cgcaacagat attatttgtg ccatctcttc
    10681 tcctcctatt tgattggctg cttattaggc agtccgggct ttcttgggta tttctttggg
    10741 gttggtttct ttttttgaat aacgatgata tgccgttgat cagagacatt cggcaaggta
    10801 accgcctctt ctttgaggaa tttaccacct aataaagcaa tcgcttgttt tgcttctgtc
    10861 aattcctctt cactttttgc tgctttcaat gcataaaaat agccttcttg tttagccaag
    10921 ggcaaacaca attccgctaa cacattcaaa cgagcaaccg cacgagcagt gacaaggtca
    10981 aatgatgcac gaaagtgagg attttgccca aatgtttctg cccggtcatg atataaggaa
    11041 gcatcgattc ctaataatcg ggttaactca gataagaagg ttattcgttt attcagagaa
    11101 tcaacgatcg ttatctgcag ttctggaaag gcaattttta aaggtatgct aggaaaccct
    11161 gcccccgaac caacatcgca taaagataaa ggttgcgtaa agtctacttc aaatgctaat
    11221 gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt catccgtaat agctgtaagg
    11281 ttcatctttt cgttccattc gactaatgtc cgataataaa tagcgaattg attcatttga
    11341 tgatcggtaa gctctatccc tttttctgcc aaggattggc gaaattcttc tggtatcata
    11401 aatattcctt tcattgtgaa acagcaattg tttcacagat tctctcccta ctttaacgca
    11461 aaatctctag acatgcaatc cttccatatg gattcttaaa aatttgaaaa gtaagaaaag
    11521 ccgatagact ttttctttct agtatgactc ataaaatata cacagaagag agaactagct
    11581 ttgagcaatt tctgcaaaaa ctagttctct cttctattta ttattacgta ttacgctggt
    11641 tcactgactt tcgcaatctt cccttgttcg atatagacca ttaggatact taggtctgca
    11701 gggtttacgc cactgattcg gctagcttgg gcaattgttt ctggttggat tttctgcaat
    11761 ttttgttttg cttctgttgc taatccgttg attgctgcgt agtcgatgtt ttctggaatc
    11821 tttttcgctt ccatgcgttt taatttttct actttttcca tggctttttt gatgtagcct
    11881 tcatatttga tttggatttc tacttgttcg atttcttttc gtgtcaattc ttcattttct
    11941 ggaataaacc gtagaagttc tgcataagta atctcaggac gtcttaaaaa gtcgcttgct
    12001 agtacaccat cttttaatgg tgccgcatta atcgttgcta aaaagtcttg gacttcttct
    12061 gttggtttga ttcgtacatt catcaaacgt ttgatttctt gttcaactgc ttcttttttc
    12121 ttcaaataat tcttgtattg atcttctttt actaagccta cacggtgacc catttctgtt
    12181 aatcgcaaat ctgcattatc atgacgtaaa agcagacggt attcagcacg tgaagtcagc
    12241 aaacgataag gttcgttcgt tccttttgtt accaaatcat cgatcatgac accgatgtaa
    12301 ccgtcacttc gtttgatcac taaaggttct tttccctgaa ctttcaaagc tgcattgata
    12361 ccggcaatca gcccttgtcc agccgcttct tcataaccgc ttgtaccatt tgtttgtcca
    12421 gctgtataaa gattttcaac gactttagtt tctaatgtcg gacggagctg gtgtggctct
    12481 actacgtcat attcgatagc atatcctgta cgcatcattt ctactttttc taatccagca
    12541 atagaatgaa gcatgttgac ttgaacatcc tctggtaaag aagtagataa accttgaaca
    12601 tagacttctt ccgtatttaa gccttctggt tccaaaaata attgatgacg tggtttgtct
    12661 gcaaaacgca cgatcttatc ttcgatggat ggacaatagc gagcgcctac cccttcaacg
    12721 atccctgtaa acattggggc acgatgtaag ttgtctcgaa taatttggtg agtcgtctca
    12781 cctgtatagg tcagccagca agattcctga tctagtttat aggcactatc tggtgtgctg
    12841 aagctaaaat gattcggctc tttgtcacca ggctgctctt ctgtaacaga atagtcgatc
    12901 gtacttgatt ttactcgagg cggtgttcct gttttgaacc gttcgatagc aaagccaagt
    12961 tcttttagat gattcgctag tccaatagaa ggttgagagt tattcggacc tgatgaatat
    13021 ttcaattcac cgataatgat ttctccgcga agtgctgttc cggctgtaat aacaactgct
    13081 ttggcccggt agctcgctcc tgtggacgtc actactccat gacaaacacc atcttcgacg
    13141 atcagttctt cgacgattcc ttgtcttagc gtcaaatttg gttctttttc gatcgtatgt
    13201 ttcatttctg ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg gacagctggg
    13261 ccttttcctg tgttcaacat acgcatttgg atataggttt tatcgatatt cttacccatt
    13321 tctccgccta acgcatcgat ttctcgaacg acaacgcctt tagctggtcc gcctacagaa
    13381 ggattacatg gcatgaatgc taccatgtcc aaatttattg tcagtaataa tgtttttgat
    13441 cccatacgtg cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc aacaacgatc
    13501 acgtcatacg catctgcttc ataatgttcc aatcgtttgt tcctcctttt attttcctaa
    13561 acaaaattga ctgaataatt gagtgattag ctcatcttgt acactgtcgc cgacaacttc
    13621 tcctagatag tcccagcaac gcgtcatatc tatttgaacg agatccacag gcattcctga
    13681 atcaatacct gcaatgactt cttcaagtga gagggaagct ttttctaaca acgcgatatg
    13741 gcgggtatta gaaacataag tagcgtctct ttctcctgtt tctccgctga agaataaatc
    13801 agaaatagct gattccaaac gatctagtcc gtcgttttta gctacggaaa ttgaaaaaac
    13861 tggttcattt tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg gcaaatctgt
    13921 tttatttaga agaatgatcc gtttcaatcc acttgtcgct tccaataatt gttcatcttc
    13981 tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc aagtctgctt ctgctaatgc
    14041 ttttcggctt ctttcgaccc cgatccgttc caccacatct tctgtttccc gaattccagc
    14101 tgtatcgatt agcttgagcg gtaccccccg aacattgaca tactcttcga ttacatctcg
    14161 agtcgttcct gcaatatccg taacgattgc tttttcttct cttaacaaat ggtttaacaa
    14221 acttgatttg ccgacatttg gccggccgat gattgccgta cttaaccctt cccgcagaac
    14281 cttcccttgc ttggatgtag caagtagcgc ttggattctt tgttggatca tctgcgcttt
    14341 ttcaagcaat aacttcgtag tcaattcttc gacatcatca tattctggat aatcaatatt
    14401 tacttctact tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga tcaaagcaga
    14461 cagattgccg tctaactgat taagagctag tcccattgct ttgtctgttt ttgcccgaat
    14521 caaatccatc acagcttctg cctgtgaaag gtcaacacga ccattcaaga atgctcgttt
    14581 tgtgaattcg cctggctcag ctaatcttgc accttctctt aacaatagct gcaaaatttg
    14641 attgactaca acgatcccac cgtgacaatt gatttctact acatcttccc gcgtaaatgt
    14701 ctttggtgct ctcatcacgg agaccatgac ttcatcgaca agctgattgc tcttgggatc
    14761 aacgatatgt ccataatgga tcgtatgact tggtacttct gataatcgtt tgtttccaga
    14821 ttgataaact ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa cgatactgat
    14881 tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta aagtaatttc
    14941 agccattttt tcacttcctt tttcattttg cgcacaaaaa aagtgcccac tccacccttt
    15001 atgcacaata aacggtgaac ttcgcacttt gactgcttgt tcttgattaa attctggtca
    15061 tagattatca cacttgctat aaggaatcaa gtgtttaaaa caattagatg aagttgagcc
    15121 tgttctgctt ccattacctt atatttaatt gtccttggat gtctctttgc gttctttggt
    15181 ttattcgaag agaagatact gtataaacaa ggctttatct ttcttaaaaa aaacggtaca
    15241 ctaaatggta gcgctatcta aactatacac ttttgaaagg agaaaatatg tgagcccgtt
    15301 cagatctgct gcattggaag aagaaagtga ccctcggatc aacgatgtcg ttttatctac
    15361 agggaatatc aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg aatgcttaga
    15421 agctgatttg tttgacccat cttttcccaa agatgacctt ttcttaacaa cgaagaggaa
    15481 attaaagacg aaggtacttg aatatggtgc gaatgcagta atcaactgtc attttgagta
    15541 tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt tttgcttatg gaacagtgat
    15601 ccaatttatc caaacaacga ttggttaaat gaaacagaca aaaaaagcgg atcaaaactg
    15661 aagacagttt cgatccgctt ttttctatca aagttagtga ctacttcatc gtatactctg
    15721 ataagaatga tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac gtgatttatt
    15781 caaaaagtaa tggaatcaat aatatttttt agcaggttct actactaaat aacgataagg
    15841 ttcctcacct tccgaatgtg tttttacatg ttcatctttg cttaatgcag catggatttg
    15901 tttacgttca aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc gttttacttt
    15961 ttcagccgtg cgatcagcta aacgctgaat gatctcttgg cgcttttcac ggtaattgcc
    16021 tacattgact actacagaca gcttatttct tgctaaacga tggatgaaga cttgtgccag
    16081 atattgcaat gcattcagtg tttttccatg tttcccaatc agtatgcctt gcttttgtgt
    16141 ttctaaatgg aaaacaacca tattatttga ttctctttcc attttgacaa gagctggagc
    16201 atcaagctgt tttgaaattt cagtaagata aactgccaat tccgttaaag ctgcttcatc
    16261 acctagctca cttacttctt ctgttttttc ttcaagaatc gtttcgttga ctacctcgat
    16321 ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc tgttctactg tttcagaaac
    16381 tttttcgctg atacttggct ggatcgaaag ccgtgcattc tttcgaccca ttcctaggaa
    16441 accttttttt ccttcttcca gtatttcgat ttcgacttgg tctttggcca aacccaaatc
    16501 agccagacct agttggacag cttcgtctat tgttgcagct tcataaatcg gcattagcat
    16561 tacctcccct attatttccg ttttttctta ggattcattg ctttcttaag agcacgttct
    16621 ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc gaattttgaa agggttgttg
    16681 atcagcagcg tttgtacaac ttggaaggca ttggaaacta cccagtataa tgaaagaccg
    16741 ctggctaaat tgacacccat cactaaaatc attacaggca tcacgaaatt catgatcttc
    16801 attgatgcat tcgattcaat ttgactcatg ctagataaat acgtacttgc aaaagtaaag
    16861 attgctgcta aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt caaccataag
    16921 aaatgccctt gtttcaactc tggtacacgt gaaatagatt gccataacgc catcaagatt
    16981 ggcatttgta ctaataaagg aagacaacca gcatatggat tgacgccatt ttctgcatat
    17041 aaacgctgct gctcttctct taacttactt tgtgtttccg gatctcttga actatattgt
    17101 tgctgcagtg cttttaattt cggttgtaat tcttgcgttt tacgcatgct ctttgtttgg
    17161 aaatgcatca acggcaatag gatcacgcgg atgatcagtg taaataaaat gatccctatc
    17221 cctgcattac caaatgacaa cgccttgatc gcctcagcaa agtaataaac aatataacga
    17281 tcccaaattc ctgtactctg tgcgctgact tccccagttc cgcaaccgga taagacgatg
    17341 accaaactaa cgataccggc catcaaaagt aaacgtttgt atttcttcac ttactctttt
    17401 cctctctgtc gattattttt gcaagtttta agacgtggac taaattggag cgaacctcat
    17461 cataagtcaa ccccttcaca cttggtctag caatcacaat aaaatccaac tccggatcaa
    17521 tttcttcttt tatagaataa actgctgctc ggatttttcg tttgacttca ttgcggttta
    17581 ccgcgttgcc gacctttttt cctacagata aaccaactcg aaaatgaggc tgtcccggtt
    17641 tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga atctcccgta tggaacacgg
    17701 cttgaaactc tcgttctttt tttactcgat acgcttttct cattagtttt ctttcctttt
    17761 ctatctctca cttacgctca taatcatacc ataattacga ttgtcctttc atcatttccc
    17821 taaaaaagag tataaaaaaa accactgaaa ccccagtggc ctaagctgaa agaacttttc
    17881 ttcctttgcg acgacggcta gctaacacac gacgtccatt tttagtactc atacgtttac
    17941 ggaatccgtg aactttttga cgtttacgtt tatttggttg gtatgttctt ttcatatatt
    18001 ccacctccaa aatgttctta tctattttat aaacacagac atactttgat agtataacga
    18061 caaagtagtg attttgtcaa tataaaatag aagatcgatt gttaaaactt atccacaaga
    18121 ttattttttt ctatgggaat cctatgtgga aaaagttatg cacagggatt atccacagat
    18181 aataaaaggc tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc acaatcaggc
    18241 gatttcctgt gtattccgtc tcctaactat tggtatatct gtatttttta tccacagacg
    18301 caagaggaaa tctattggtt tttacgagtt ttccacctgt gcattagtgg tgtggaaaac
    18361 tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg ataattgtaa
    18421 ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc taaattagta
    18481 ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg atgctttatg
    18541 gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata atacatggat
    18601 cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg ttcctagcaa
    18661 gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag tcggttatat
    18721 gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag agcaagaaga
    18781 agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa aagccatgct
    18841 gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga tggctcatgc
    18901 agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct tcttttatgg
    18961 aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa tgctgcaaag
    19021 tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg atttcatcaa
    19081 ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg ttgatttact
    19141 gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag agtttttcca
    19201 tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg atcggttgcc
    19261 aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg ggctgtctgt
    19321 tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa aagcggcggc
    19381 tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa ttgattctaa
    19441 cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga tgaatagtga
    19501 agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag gaaaaggaca
    19561 tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc acatccaatt
    19621 gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac aaatcgctat
    19681 gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg aattcggtgg
    19741 caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga tggattctag
    19801 tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt aatttgtcga
    19861 ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag ttatacacac
    19921 tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc acagaatcaa
    19981 catgccctat tacttttatt attcttttta actaataata aataaacaaa cattcaaagg
    20041 agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt gcaaactgtt
    20101 caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa aatcacactg
    20161 acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga aacttttttg
    20221 tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat tgttttacaa
    20281 gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac tttagaagtt
    20341 ttagaaaata aacaagtagc gatcacttct ggaaaagcga attttatcgt aaatggatta
    20401 gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat gaaattacct
    20461 gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca acatgagagt
    20521 cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt agctgtagct
    20581 actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc tgatcatttt
    20641 gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac aaatgaagaa
    20701 gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga aacgatgtat
    20761 ttctattccc gtttgctaga aggaaactat ccagatacca atcgtttgat tccttcaagt
    20821 tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga acgggcttct
    20881 ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc agatgcggtt
    20941 gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag ttatacagca
    21001 agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc agcgttgcgt
    21061 gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc atttacattg
    21121 gaaccaacgg aagatggcgt tcaatttatt cagctgatta ctcctgtacg tacaaattaa
    21181 caatgatttt caatgttctt gaaaatttaa ttgaaaagaa atagaaaaaa taaaatttta
    21241 aacagaaggc cctaaaatct cttttaaggc tttctgttta tttttgttta aatcaccttt
    21301 tttagattaa atcgtcttat atggaaaaat atatggataa atttgtttta ttgcgaaaaa
    21361 aaaggtataa tagagtatac ttttaataat gaaaatgagg gatgggcatt gaaacaacaa
    21421 gtcattctaa atacggaatt catgacgttg ggccaaatgt tgaaagaagt cacagttatc
    21481 ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt cgatggcgaa
    21541 ccagaaaatc gtcgtggacg caaactttat cccggcatga tggttgaagt tccagaagta
    21601 ggaacttttt ttatggtaaa aaaaggaaac gacgataatg aggctgaata agctgtattt
    21661 aaaaaattat cgaaattacg aagagctgaa catcgaattt tccaagaacc tcgtcatctt
    21721 tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg tattagctat
    21781 gacgcgaagt catcggacaa gtagtgagca ggaattgatt ggctggactg acgatcaggc
    21841 gatgattcaa ggagagatca caaaaggttc atccactctt ccattagaaa tcttattatc
    21901 taaaaaaggc cgaaaaacca agatcaacca tatcgaacag aaaaagttaa gtacttacgt
    21961 gggacaaatg aatgtcattt tatttgcacc agaagatctt tctttagtaa aaggcagccc
    22021 gcagatcagg cggaagtttt tagatatgga attaggccag atcaatccaa tctatcttta
    22081 tgatcttgtc caataccaag caattttgaa acaaagaaac cagtacttga agcaattgaa
    22141 tgaaaaaaaa caaactgatt tgctttattt ggatattttg tccgaacaac tagcagcgtt
    22201 tggcagcaaa gtattgaaag caagagcaca gttcgtcaag cgattggagt tttgggctaa
    22261 ttctttgcat cagcaaatca cccatcaaaa agagcagctg gaaatcgagt atctcaccgc
    22321 agtcgattct ttagaaacac atacgcaaga gcaaatccaa gaacaatttc tcgcactttt
    22381 gaaccaaaat aaaaagaaag acctttttcg gggaacaacg acagttggtc ctcatcgtga
    22441 cgatctttcc ttttttatta atcagaaaaa tgtgcagact tatggatcgc aaggacagca
    22501 aagaacaact gcactaagtg tcaaattagc agaaatcgat ttgattaaag aagagacagg
    22561 ggaatatcca atccttttgt tagatgatgt gatgagcgag ctggatgata atcgtcaact
    22621 ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa cgacacttga
    22681 ccatgtaaaa aataaaatga ctgttgaacc tgagattttt tatgttcaac aagggaaact
    22741 aaaaggagaa gaaactgaat gacagaagaa agaagtttag tagaacgcgc taaagagtat
    22801 gatgccagtc agattcaagt acttgaagga cttgaagccg ttcgtaaacg acctggcatg
    22861 tatatcggtt cgactagttc tgagggtctg catcaccttg tttgggaaat tgtcgataat
    22921 tcgatcgatg aagtattagc aggttttgca acgaaaatcc atgtgattat tgaaaaagac
    22981 aacagtatca ccgttattga cgacggtcgg gggatacctg tagatatcca ggcaaaaact
    23041 ggacgtcctg cagttgaaac ggtctttact gttttacatg ctggaggaaa attcggcggt
    23101 ggtgggtaca aagtttctgg tggacttcac ggagtaggat cttctgttgt aaacgcgctt
    23161 tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca agaatatcgt
    23221 cgtggagctg tagtggacga cttaaagatt atcgaacaaa cggatcgcca tggaacaacg
    23281 gttcacttta cgccagaccc tgaaattttt acagagacaa ctgagtttga tttttcaaaa
    23341 ttggctaccc ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat atcgattgaa
    23401 gacaaacgag aagaagaacc agtcatcaat gaataccatt atgacggcgg gatcaaaagt
    23461 tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc cagaacctgt atatttggaa
    23521 ggtgaacagc aagatatcgc agttgaagtc tctatgcaat atacagacgg ctaccattcc
    23581 aatatcatga gttttgccaa caatatccat acgtatgaag gtggaacaca tgaatcagga
    23641 ttcaagactg ctttaactcg tgtgataaat gattatgccc gaaaacaaaa gctgatgaaa
    23701 gaaaatgacg acaacttgac cggtgaagac gtgcgagaag gattgacagc tgttatttct
    23761 atcaagcatc ctgaccctca attcgaagga caaacaaaaa caaaattggg caattccgaa
    23821 gtacggacag tcactgatcg gttatttagc gaacatttca tgaaattctt gctggaaaac
    23881 ccaagtgtgg gccggcaaat tgttgaaaaa ggattactag cttccagagc tcgtctagca
    23941 gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt tagagattag taatctgcca
    24001 ggaaaattag cggactgctc tagcaacgat cctgaaaaat gcgaattgtt catcgtcgaa
    24061 ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc gagagtttca agccatcttg
    24121 cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca tggacaagat cttggcaaat
    24181 gaagagatcc ggtctttatt tactgctatg ggtacagggt ttggagaaga ttttgatgta
    24241 tcgaaagctc gctaccacaa attagttatc atgactgatg ccgatgtcga cggcgcgcat
    24301 atccgtacat tgctgctgac tcttttttat cgttacatgc gtcctattgt tgaagctgga
    24361 tacgtctata ttgctcagcc acctttatac ggggtaaaac aagggaagaa tattacgtat
    24421 gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag tcgttgcttc acttcctgcc
    24481 tcaccaaaac caagcgttca gcgttataaa ggacttggag aaatggatga tcatcaacta
    24541 tgggaaacaa ctatggaccc aagcaatcgg atgatggccc gcgtcagtgt ggacgatgct
    24601 attgcagcag atcagatatt tgaaatgttg atgggggatc gagtagaacc ccgtcgtgca
    24661 ttcatcgagg aaaatgcaca ctatgtgaag aacttagata tttaatcatg aaggaggaaa
    24721 aagaatgagt gaagaaatca gagaaaacat ccatgatgtc aatctaacca gcgaaatgaa
    24781 agactccttc atcgattatg cgatgagtgt tattgtcgca cgtgcacttc ctgatgtcag
    24841 agatggtctg aaacccgttc atcgccgtat tttatacggc atgaacgaat taggtgtaac
    24901 accggataaa gcccataaaa aatctgccag aatcgtcgga gatgtcatgg gtaaatacca
    24961 tcctcatgga gacattgcga tctatgagtc aatggtacgg atggcacagc cattcagcta
    25021 ccgttatatg ctagtggatg gtcatggaaa ctttggttct gttgacggtg acggtgccgc
    25081 cgctatgcgt tataccgaag cacggatgag caaaatcgct acagaaatgc tgcgtgatat
    25141 caataagaac actgttgatt tccaaagcaa ctatgatgat acagaaaaag aaccagttgt
    25201 actacctgct cgcttcccga atcttttagt gaacggaaca acggggatcg ccgtaggtat
    25261 ggccacgaat atcccacctc ataacttgtc tgaggtagta gcggctatcg atttgctgat
    25321 ggataatcct gatgtgacga ccaatgaatt gatggaagta ttacctggac cagattttcc
    25381 aacaggtggt ttggttatgg ggaaatcagg aatccgaaga gcgtatgaaa caggtaaggg
    25441 ttctattacc gtacgtgcaa aagttgagat tactgagatg ccaaatggta aagaacggat
    25501 catcgtgaca gaattgcctt atatggtcaa taaagcaaaa ttgatcgaac ggatttctga
    25561 actgcacaga gacaagcgta tcgaagggat cacagatttg cgggacgaat cttcccgtga
    25621 aggtatgcgg atcgtcattg acatccgtcg tgacgccagc gcatccgtta tcttgaataa
    25681 cttatataaa ttgacatcct tgcaaaattc atttggattc aatatgttgg cgatcgaaaa
    25741 aggtgtgccg aaagttttga gcttgaaaca aatccttgaa aactatatcg aacatcaacg
    25801 cgaagtcatc acacgacgga cggtctttga aaaagaaaaa gcagaagcaa gagcacacat
    25861 cttagaaggt ttgcgtattg ctttggatca tatcgatgaa atcattgcga ttatccgcgg
    25921 gtctaaatca gatgacgagg caaaagcaac aatgatcgaa cggttcgatt tatccgatcg
    25981 ccagtctcaa gctattttag atatgcgttt acgccgattg accggtttgg aacgggaaaa
    26041 aatcgaaaat gaataccaag aactcttaaa actgattgaa gatttaactg acattctggc
    26101 aagaccagaa cgtgttacag aaatcatcaa gaatgaatta gcagacttga atcaacgatt
    26161 tggtgataaa cgacgcacag aattactagt cggtgaggtc ttaagtctgg aagatgaaga
    26221 tttaatcgaa gaagaagaaa tcgttattac attgacaaac aatggttaca tcaaacgttt
    26281 ggcaaataat gaattcagag ctcaacgaag gggtggacgt ggtgtccaag gaatgggtat
    26341 ccacaatgac gacttcgtca aaaatctggt ttcttgttct acccatgata cattgttatt
    26401 ctttacgaat aacggaaaag tgtatcgggc aaaaggatac gaaattccag aatacgggcg
    26461 tacagccaaa ggaatcccaa tcatcaattt actaggtatc gattcgagtg aaagcatcca
    26521 ggcaatcatt gcggtgacgg gagaagcaga agaaggccac tatctcttct ttacgacgcg
    26581 tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct aacatccgaa gtaatggtct
    26641 gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat gtgctactga cagatggtca
    26701 atctaatatc attattggaa cacataacgg ctactctgtc actttcaaag aagaagcagt
    26761 ccgcgatatg ggacgtacag cttccggggt cagagggata cgcctgcgtg aggaagatta
    26821 tgtcgttggg gcagccttaa tgaaggaaaa tcaagaagta ttagtgatca cagaaaaagg
    26881 ttacggaaaa cggacaaaag tatcggaata tccagtgaaa ggccgcggag gtaaagggat
    26941 caaaacggcg aacatcactg aaaaaaatgg tccactagct ggattgacca ctgtttctgg
    27001 agatgaagac atcatgctga ttacagataa aggtgtcatt atccgtttca acgtatcgac
    27061 tgtttctcaa actggacgtt caacattagg cgtacgtttg atgaagatgg aagctgatac
    27121 gaaagttgtc acgatggcag cagtcgaacc agaagctgca gaacaaacag ttgaagaaca
    27181 aggatcagaa gaataaccat caagactaag tgtgtaagct agcaagaata accgattaaa
    27241 aaatctggtt attcctgctg gcttttttga ttagttgaat ttctcttaaa atattcgaaa
    27301 ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat aattagagct tgtgagtagt
    27361 ctaattaatt agataactct ccttgctctt aggaacctaa gagccgaaag tccacaggga
    27421 ggtgaatatc aatggaaaac acgaaatatg aaatcatgta cattattcgt ccaaacattg
    27481 atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc aatcttgaaa gataacggag
    27541 cagaagttat cgaatcaaaa gattgggaaa aacgccgttt cgcttatgaa atgaacggtt
    27601 accgtgaagg tatctatcac atcgttaaag tatcttctcc atcatcagca aatgctgtca
    27661 atgaatttga ccgtcttgct aaaatcaacg acgatatcat tcgtcacatg attgttaaag
    27721 aagaagctta attgttgttt catgtgaaac aatagaaaac gatgagagga gatgactatt
    27781 ttgattaata atgttgtatt agttggacgt ctgacgaaag atccagattt gcgttacaca
    27841 gcaagtggta cggcagtcgc aacatttact ttggctgtga atcgtaattt tacaaaccaa
    27901 agcggcaatc gcgaggctga ttttatcaac tgtgtgattt ggcgcaaatc agcagaaact
    27961 ttagcaaact atgctcgtaa aggaacactt ttaggtgtta ctggacgtat ccaaactcgt
    28021 tcttatgata atcagcaagg acaacgtgta tacgtgacag aagtagtagc tgacaacttc
    28081 caattgctgg aaagtcgttc tgcatctgaa aatcgccaac aaagcggcgg attccaaagt
    28141 tctggtcaat cagctggcgg atttggcgga aacaataact ctaatcaaac atctcaatca
    28201 tccaacggta tgcctgattt cgaccgtgat acttctgacc cattcggctc aagttcaacg
    28261 atcgacattt cagacgatga tttaccattc taaacgaatt tgataggagg gaaatagaat
    28321 ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta gactacattg ctgctaacca
    28381 tattgaatat atcgattata aagatatcga attactaaaa agatttattt cagaacgcgg
    28441 taaaatcttg ccacgtcgtg taactggtac aggcgctaaa aaccaacgta aaattactgt
    28501 tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc gttagcgaag actaataaat
    28561 cctaggaatt gaatgactga tccttttgat cagctattca attccttttt ttctgtaaaa
    28621 ataacctcta ttcgtttagt cgctcgaatg acgaaagtta tggtaaaatc aaattagtca
    28681 tatatgggag gaaaattatg gaaaataaaa acaatcattt atccccgaaa cctctcttcc
    28741 ttatcctgtt tatattaatc gaagtattgg tagtcttaat tttaccattt cgttttgtta
    28801 gcgcttcggt cattctattg atgaatttat ggggtgttcg ctattggttg aaaatgaaac
    28861 gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc ttcgactgta gctgaaacca
    28921 atctagctta tgtctcagaa gtcatgcctg taggagtgat tgcttatcag ccatcaagcc
    28981 aaaaaatcga atggctcaat ccattagcat tgaaaatcaa agatcaaagt ggattaacag
    29041 aggaagaatt gctagatact ttttttactg caaaagaaaa aaagaaaaac caagtctatc
    29101 tagcagataa aacatttcag tttgatatgg atacagtaaa gggtgttgtt tattttaatg
    29161 atattaccca aaaccagttg ttaaaactcc atcagacgaa cagtcagccg gtgattggta
    29221 ttttatctat tgataactat gcagatgcca ttgataaatt agatgacaaa gagatttctt
    29281 atttgaactc tttcgtcact acactagttt ctgactggat gaatgaatac catgtctttt
    29341 tcaaaagaat caacgcagaa cggtttttct tcgtagcaca gatgatcgat ctgaaaaaga
    29401 tgatggataa aaaatttgat ttgctggatc gctttcgagc agaagctgaa aaacagagct
    29461 cgcctatcac ccttagtatg gggattgctt atggctcaag aaccctcgat gaaatcggtg
    29521 gagaagctca gaataattta gatgttgctt tagtccgtgg aggcgaccaa gttgtcgtga
    29581 aagaggtaaa agaaggagca aaacctgttt attatggggg gaaatctgct aagaatgtca
    29641 aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt gcgtcggatt tttgatgaaa
    29701 caggcgatgt gtttatcatg gggcacaagt ttccggatat ggatgcttta ggctctgctt
    29761 ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc ttacatcgtt atcaatgaga
    29821 acgagatcat tccagatgta gaacggtgtc ttgaagaaat ccataagcat cctgaattag
    29881 aaatgcaact aatctctcca gaacgtgcca tagagctgaa aaaagaagag gacctcctcg
    29941 tgatggtgga ttaccataaa ccttctctat cgatctctca gggcctatat gagcaatttg
    30001 ataagattgt gatcattgat caccatcgtc gtggggaaga gtttccaagt aaacctctgc
    30061 tgacttatat tgaatcttca gcttcttcag cttcagagct agtttctgag ctgatccaat
    30121 accaaagcag caaaacaaat cgcttagatc gttttgaggc cacattattg cttgcaggga
    30181 tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc acgaactttt gacgttgcca
    30241 gctatttaag gtcttgtgga gcagacgcat ccttagtaca atatttattg agctcggatt
    30301 tagcgacata cttggagatc agcaatttga ttgctggaag cgactatgtg acttctgata
    30361 ttgttgtggc ggcagggaca gacgaaaaag agtataatag tgttatagca gcaaaaacag
    30421 cagatacatt gctttcgatg atcaatatca atgcagcttt cgtcattaca aagcggacag
    30481 acggcttgat tgggatcagt gccagaagta ccggcacgat caacgtccaa atcatcatgg
    30541 aatccttagg tggcggagga cacttcacaa acgcagctac tcaactggaa aatacgacag
    30601 ttgaggaaac aaaacagaag ctatttgctg caatcaaaga aaatatgaat caaatatatg
    30661 ataaggagta atccaaatga aagtaatttt tttacaagat gtaaaaggaa aaggtaaaaa
    30721 aggggacatc aaagaagttc cagcaggtta tgcacaaaat tatctaatca aaaataactt
    30781 ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca ggacaaaaga aagcacaaga
    30841 aaaacacgaa gctgaaatct tggaagaagc taaaaagtta aaagaattct tggaaaaaga
    30901 agatactgta gtcgaattga aggcgaaagc aggggaagac agccgtttgt ttggttcgat
    30961 cccttcaaaa caaattgctg aagggttaaa caaacaatac aaaatcaaat tagataaacg
    31021 aaaaatcgaa ttagaaaatc cgatccgttc actaggctac acaaacgtac ctgttaagct
    31081 tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta caagaataac aaatcttgat
    31141 agcgatgttc agctggtaag tataaccaga aggaaaaaag acaaaccgaa atttatctga
    31201 gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga tttcagattg atttcggttt
    31261 cttttttggg aacagcaaaa tagcataaag aaaacgagta taataaaaga gaactatttt
    31321 tttattacac tttcggtacg aatgatgact tttctctcat tttcttgtaa aatgaagcaa
    31381 ttgaagacaa ggagcgatac gtatgaatga agtgtggcag gatcgaatcc caccgcaaaa
    31441 tattgaagct gaacaagcgg ttttaggctc tgtttttctt gacgcagaaa cgatcattga
    31501 tgccatggag tacatagagc caaaagactt ttatcgtcgt ggccatcaaa ttatttttga
    31561 gacaatgatc gagttaaatg atcgtaatga agcgattgac gtggttactg tcaaagaccg
    31621 gctggaacaa gccaatttgt tagaagatgc cggtggttta agttatctct cagacttggc
    31681 attagcagtt ccgacagcag caaatatcgt ctactatgcg aaaatcgttg aagaaaaatc
    31741 attgctgcgt acattgatcc agacagcaac aggaatcgtt acgaaaggat tcgagcaagg
    31801 agaagaagtt caaacaattt tagatgatgc cgaacgcagt atcttggagg tttcggaaaa
    31861 aagaaatcga agcggctttt tatctattgc tgaagtactg aactccacga ttgaaaatat
    31921 cgatcgacta gctcaaaaca atgaagaaat cacaggtcta ccaactggct atcaaggtct
    31981 ggataaaatg acagcaggtt tccagcccga agaactaatc attcttgctg cacgtccagc
    32041 ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat gtaggaacaa aaacagatcg
    32101 ttctgttgcg atatttagtt tggaaatggg ggcagaatct cttgtaaatc ggatgctttg
    32161 tgcagaaggt tcgattgagg ccagccattt acgtacaggt cagctttcgg aagaagaatg
    32221 gtcgaatctg atcgttgcga tggggagtct ttctcgagca aatattttta tcgatgatac
    32281 accagggatc aaaatctctg aaatccgtgc gaaatgtcgg aaactagcgc aagaaaaagg
    32341 caatctaggc ttgatattga tcgattattt gcagttgatt gaaggtaacg gtaaagagaa
    32401 ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa aaactggcga aagagctgaa
    32461 agtacctgtc attgctcttt cacagttatc tcgtggagta gaacagcgac aagacaaacg
    32521 accagtgcta agtgatattc gtgaatccgg atcaattgaa caagatgcgg acatcgtagc
    32581 cttcttgtat cgagatgatt attatgaaag agaaggcgga gatgacgatg atggaaacga
    32641 gccaaaaaat gacaacgtca tcgaagtcat catcgagaag aaccgaagcg gtgcacgagg
    32701 aacagttgaa ctactgttta tcaaagaata caataagttt tcgtcgcttt cgccaagaga
    32761 agaattttaa agaagaggtt gtgagccttg agcaatcccg agagctctcg taagaagatg
    32821 caaggagtaa ccgcaggaac gactagagta cttaatttaa acagatctcc aaagggattg
    32881 tgacaaaagt cttgtcacag tccctttttc cgaataaacg gtgttcaatc agctgaccct
    32941 tggtattagc tcagcaaacg tcaaaatatg aggaactatt ttgacgtttg tttctctaat
    33001 actcaggtca aaacgctgat cttacgacct cttttcctta aacggtgttc tcctgccaac
    33061 tcctcggtca caagtcacaa tatcatcaaa catgagaagc agttttttga aattgctcct
    33121 tgtgactcag agttaaacgg caggctcaca acctctttct taaacggtgt tcaaaagctt
    33181 gcatctgcgg taatacgccc aatcaaacga aaaatatgag aagctatttt tgtttggctg
    33241 ttcttattac ttgatgcagg acagcttttt cacaacctct gattcacggt gttccaaaag
    33301 tagcttcttc gaaaaataag tcgaaatttc acaaaaattt gagaagcaat tttcgtacat
    33361 tccttcttat ttcttgaagc tgaccacttc tgttacaacc tcttttttaa taaccattct
    33421 aaaaagataa agttcgtttt ttaagcgttt ttataaaata aaaataaaaa tcattcgatt
    33481 ttatttgaaa tatgtatcta ttattgatac aatggttcag gattaaataa tatagaacga
    33541 ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat ggggcgatga agggaaagga
    33601 aaaatcacag actttctcag tgaaaatgct gaagtgattg cacggtacca aggtggcgac
    33661 aatgcagggc acacaattaa gtttaacggt gtgacttata aactgcattt gattccttct
    33721 gggatttttt ataaagacaa gatcagtgtg atcggaaacg gcgttgtagt caatcctaaa
    33781 tcattggtta aagaattagc ttatttacat gaaaaaggtg tagaaacaag taatttgcgt
    33841 atttctgacc gcgcgcacgt aatcttgcct tatcatatca aattggacca attacaagaa
    33901 gatgctaagg gcgaaaataa gattggtaca acgattaaag gaatcggacc tgcctatatg
    33961 gataaagcag ctcgtgtagg tatccgtatt gcagacttat tagataaaga aatttttgaa
    34021 gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat ttgtaaaaat gtttgattca
    34081 gaaccacttg attttgacga tatttttgaa gaatattatg aatacggaca acaaatcaaa
    34141 caatacgtca cagatacttc cgttatcttg aatgatgctt tggatgcagg caaacgcgtg
    34201 ttgtttgaag gcgcacaagg tgtaatgctg gatatcgacc agggaactta tccttttgtt
    34261 acttcttcta acccagtagc cggtggggta acaatcggaa gcggtgtagg gccttcaaaa
    34321 atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac gtgtcggtga tggaccattt
    34381 ccaactgaat tatttgatga aacaggagaa caaatccgcg aagtaggacg agaatacggt
    34441 acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt ctgtagttat gcgccattct
    34501 aaacgagttt caggtattac caacctatct ttaaactcga tcgatgtatt aagtggattg
    34561 gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg aactaatcta ccactatcca
    34621 gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg aagaattgcc aggctggtca
    34681 gaagacatta caggatgcaa aacacttgca gaactgccag aaaacgcacg taactatgta
    34741 cgtcgtatct cagagcttgt tggtgtacgt atctcaacat tttctgtcgg acctgataga
    34801 acacaaacaa acatcttaga aagcgtctgg gcgcaaatct aaggataaaa acgacgactg
    34861 aatcaggacg ttcttaccag agcgagtcga agtgtttatc tatacgataa taataacttg
    34921 aagaggtagt gaaagagacg tttagcctta ttgccgatag gctgtctctt gaacactgtt
    34981 tattcagaat aagggactgt gacaaaaacc gtgtcacagt ccctttttcc gaa
//
LOCUS       DAFCHQ010000019        34962 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000019 DAFCHQ010000000
VERSION     DAFCHQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.019"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="LNC71_001029"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="LNC71_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBK6466116.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="LNC71_001030"
     CDS             complement(2020..2922)
                     /locus_tag="LNC71_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6466117.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="LNC71_001031"
     CDS             complement(2919..3611)
                     /locus_tag="LNC71_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBK6466118.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="LNC71_001032"
     CDS             complement(3654..4295)
                     /locus_tag="LNC71_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6466119.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="LNC71_001033"
     CDS             complement(4333..4647)
                     /locus_tag="LNC71_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466120.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="LNC71_001034"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="LNC71_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="LNC71_001035"
     CDS             complement(5984..6916)
                     /locus_tag="LNC71_001035"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBK6466121.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="LNC71_001036"
     CDS             complement(6913..7689)
                     /locus_tag="LNC71_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBK6466122.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="LNC71_001037"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="LNC71_001037"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBK6466123.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="LNC71_001038"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="LNC71_001038"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBK6466124.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="LNC71_001039"
     CDS             complement(12014..13294)
                     /locus_tag="LNC71_001039"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBK6466125.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="LNC71_001040"
     CDS             complement(13313..14239)
                     /locus_tag="LNC71_001040"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBK6466126.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="LNC71_001041"
     CDS             complement(14252..15535)
                     /locus_tag="LNC71_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBK6466127.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="LNC71_001042"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="LNC71_001042"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBK6466128.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="LNC71_001043"
     CDS             complement(16323..17234)
                     /locus_tag="LNC71_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6466129.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="LNC71_001044"
     CDS             complement(17231..17695)
                     /locus_tag="LNC71_001044"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBK6466130.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="LNC71_001045"
     CDS             complement(17700..18398)
                     /locus_tag="LNC71_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6466131.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="LNC71_001046"
     CDS             complement(18385..18771)
                     /locus_tag="LNC71_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466132.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="LNC71_001047"
     CDS             complement(18918..20588)
                     /locus_tag="LNC71_001047"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBK6466133.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="LNC71_001048"
     CDS             20771..21004
                     /locus_tag="LNC71_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBK6466134.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="LNC71_001049"
     CDS             complement(21103..21534)
                     /locus_tag="LNC71_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBK6466135.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="LNC71_001050"
     CDS             21613..22026
                     /locus_tag="LNC71_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBK6466136.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="LNC71_001051"
     CDS             complement(22064..23212)
                     /locus_tag="LNC71_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6466137.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="LNC71_001052"
     CDS             complement(23334..23930)
                     /locus_tag="LNC71_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6466138.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="LNC71_001053"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="LNC71_001053"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBK6466139.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="LNC71_001054"
     CDS             27160..27591
                     /locus_tag="LNC71_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466140.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="LNC71_001055"
     CDS             complement(27636..28985)
                     /locus_tag="LNC71_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6466141.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="LNC71_001056"
     CDS             complement(29055..30218)
                     /locus_tag="LNC71_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK6466142.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="LNC71_001057"
     CDS             30347..30670
                     /locus_tag="LNC71_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466143.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="LNC71_001058"
     CDS             complement(30853..32202)
                     /locus_tag="LNC71_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6466144.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="LNC71_001059"
     CDS             complement(32271..33224)
                     /locus_tag="LNC71_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBK6466145.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="LNC71_001060"
     CDS             complement(33326..34516)
                     /locus_tag="LNC71_001060"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBK6466146.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9952 a         7642 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DAFCHQ010000020        34553 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000020 DAFCHQ010000000
VERSION     DAFCHQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.020"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..845)
                     /locus_tag="LNC71_001061"
     CDS             complement(<1..845)
                     /locus_tag="LNC71_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6466147.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDS"
     gene            complement(1069..3009)
                     /gene="thrS"
                     /locus_tag="LNC71_001062"
     CDS             complement(1069..3009)
                     /gene="thrS"
                     /locus_tag="LNC71_001062"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBK6466148.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3346..3525)
                     /locus_tag="LNC71_001063"
     CDS             complement(3346..3525)
                     /locus_tag="LNC71_001063"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBK6466149.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3572..5395)
                     /gene="cadA"
                     /locus_tag="LNC71_001064"
     CDS             complement(3572..5395)
                     /gene="cadA"
                     /locus_tag="LNC71_001064"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK6466150.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5673..7952
                     /locus_tag="LNC71_001065"
     CDS             5673..7952
                     /locus_tag="LNC71_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6466151.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8045..8692)
                     /locus_tag="LNC71_001066"
     CDS             complement(8045..8692)
                     /locus_tag="LNC71_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466152.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8871..9590)
                     /locus_tag="LNC71_001067"
     CDS             complement(8871..9590)
                     /locus_tag="LNC71_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6466153.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9721..10872)
                     /locus_tag="LNC71_001068"
     CDS             complement(9721..10872)
                     /locus_tag="LNC71_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBK6466154.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11089..11829)
                     /locus_tag="LNC71_001069"
     CDS             complement(11089..11829)
                     /locus_tag="LNC71_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6466155.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11833..12156)
                     /gene="rsfS"
                     /locus_tag="LNC71_001070"
     CDS             complement(11833..12156)
                     /gene="rsfS"
                     /locus_tag="LNC71_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBK6466156.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12213..12806)
                     /gene="yqeK"
                     /locus_tag="LNC71_001071"
     CDS             complement(12213..12806)
                     /gene="yqeK"
                     /locus_tag="LNC71_001071"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBK6466157.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12790..13440)
                     /locus_tag="LNC71_001072"
     CDS             complement(12790..13440)
                     /locus_tag="LNC71_001072"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBK6466158.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13450..13761)
                     /gene="yhbY"
                     /locus_tag="LNC71_001073"
     CDS             complement(13450..13761)
                     /gene="yhbY"
                     /locus_tag="LNC71_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBK6466159.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13777..14886)
                     /gene="yqeH"
                     /locus_tag="LNC71_001074"
     CDS             complement(13777..14886)
                     /gene="yqeH"
                     /locus_tag="LNC71_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBK6466160.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14883..15410)
                     /locus_tag="LNC71_001075"
     CDS             complement(14883..15410)
                     /locus_tag="LNC71_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBK6466161.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15590..16375)
                     /locus_tag="LNC71_001076"
     CDS             complement(15590..16375)
                     /locus_tag="LNC71_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBK6466162.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16368..17237)
                     /locus_tag="LNC71_001077"
     CDS             complement(16368..17237)
                     /locus_tag="LNC71_001077"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBK6466163.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17238..18617)
                     /locus_tag="LNC71_001078"
     CDS             complement(17238..18617)
                     /locus_tag="LNC71_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBK6466164.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18625..19044)
                     /gene="fabZ"
                     /locus_tag="LNC71_001079"
     CDS             complement(18625..19044)
                     /gene="fabZ"
                     /locus_tag="LNC71_001079"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK6466165.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19069..19545)
                     /gene="accB"
                     /locus_tag="LNC71_001080"
     CDS             complement(19069..19545)
                     /gene="accB"
                     /locus_tag="LNC71_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBK6466166.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19548..20783)
                     /gene="fabF"
                     /locus_tag="LNC71_001081"
     CDS             complement(19548..20783)
                     /gene="fabF"
                     /locus_tag="LNC71_001081"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBK6466167.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20802..21539)
                     /gene="fabG"
                     /locus_tag="LNC71_001082"
     CDS             complement(20802..21539)
                     /gene="fabG"
                     /locus_tag="LNC71_001082"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBK6466168.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21551..22468)
                     /gene="fabD"
                     /locus_tag="LNC71_001083"
     CDS             complement(21551..22468)
                     /gene="fabD"
                     /locus_tag="LNC71_001083"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK6466169.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22575..22799)
                     /locus_tag="LNC71_001084"
     CDS             complement(22575..22799)
                     /locus_tag="LNC71_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK6466170.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22852..23814)
                     /locus_tag="LNC71_001085"
     CDS             complement(22852..23814)
                     /locus_tag="LNC71_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBK6466171.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23824..24264)
                     /locus_tag="LNC71_001086"
     CDS             complement(23824..24264)
                     /locus_tag="LNC71_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6466172.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24570..25535
                     /locus_tag="LNC71_001087"
     CDS             24570..25535
                     /locus_tag="LNC71_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBK6466173.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25592..25822)
                     /locus_tag="LNC71_001088"
     CDS             complement(25592..25822)
                     /locus_tag="LNC71_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBK6466174.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25908..26795)
                     /locus_tag="LNC71_001089"
     CDS             complement(25908..26795)
                     /locus_tag="LNC71_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBK6466175.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26884..28107)
                     /locus_tag="LNC71_001090"
     CDS             complement(26884..28107)
                     /locus_tag="LNC71_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6466176.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28124..29170)
                     /locus_tag="LNC71_001091"
     CDS             complement(28124..29170)
                     /locus_tag="LNC71_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6466177.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29334..31061)
                     /gene="ptsP"
                     /locus_tag="LNC71_001092"
     CDS             complement(29334..31061)
                     /gene="ptsP"
                     /locus_tag="LNC71_001092"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBK6466178.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31061..31327)
                     /locus_tag="LNC71_001093"
     CDS             complement(31061..31327)
                     /locus_tag="LNC71_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBK6466179.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31806..34040
                     /locus_tag="LNC71_001094"
     CDS             31806..34040
                     /locus_tag="LNC71_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6466180.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34188..34508
                     /locus_tag="LNC71_001095"
     CDS             34188..34508
                     /locus_tag="LNC71_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBK6466181.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9753 a         7482 c         6035 g        11283 t
ORIGIN      
        1 ttcgaatcca taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc
       61 ttttcgtttt tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa
      121 agtgaattag ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt
      181 acatcggcta tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata
      241 ttcatgcttt tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa
      301 acaagccaaa gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata
      361 tctttttcaa aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact
      421 actaaagaag aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct
      481 acgacttttt ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt
      541 gcattgttac caagtttgtt tttgatagag taaaaggact catttgcttc tatatcacta
      601 aaaccttcat cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga
      661 ctttgaaaac cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct
      721 tctgctaatt cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg
      781 gccaactttt tttctgctga agaataatct tccatcatac tttcaatacg actaattacg
      841 ctcataagtt cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt
      901 attcacggtc gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttat
      961 cccatcaatc ccacagattc tattgagcat ctgtgtcttt gaggaatacc tgttatctat
     1021 tgttcggatt gctttctatc tactactttt ttacaggaaa tttacctatt agttttctct
     1081 gctgtaattt ttgacttctg cttccatagc atcaataaag atattcaaat cttctacagc
     1141 tgtttcttta cttccataac gacgtacgtt cactgttgca tcttccaatt ccttgtctcc
     1201 aacaactaat tggtaaggga tcttttgtgt ttgagaggca cgtatcttat agcccatttt
     1261 ttcattacga tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc
     1321 ataagcataa tcgccatgtg catcaacaga aacagggatg attgttgctt gtataggtgc
     1381 caaccatgtt ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa aacgttccat
     1441 tgtagaaaca attccgcggt ggataactac tggacgatgc gtattttccc cgtcttctcc
     1501 aacatatgtc aaatcaaagc gctctggcaa taagaagtct aattgtatag ttgataatgt
     1561 ttcttctgta ccaagagctg ttttgacttg aacatctagt tttggtccgt aaaaggctgc
     1621 ctcgccttct gcttcaaagt aatctagttc taattcatcc atagcggctt tcagcattgc
     1681 ttgggctttt tcccacattg cgtcatcatc aaaatattta tctgtattgt ttggatcgcg
     1741 atagctcaag cggaaacgat aatctgtaat attgaagtca gcatatactg ccaccatcaa
     1801 ttcaagcgta cgtttaaatt catctttgat ttgatcagga cgaacaaatg tatgaccatc
     1861 attcaacgtc atttctcgta cacgttgtaa acctgacaaa gcacctgatt tttcatagcg
     1921 atgcatcatt cctaattcag cgatacgaat tggtaattca cgataagaat ggatcgtgtt
     1981 tttgtagacc atcatgtgat gagggcagtt cattgggcga agaacaagca tttcgccatc
     2041 tcccatatcc attggcggga acatgtcttc ttgataatga tcccagtgtc cagatgtttt
     2101 gtacaactca acatctccca ttattggtgt atatacatgt tggtaaccta gactaacttc
     2161 tttgtctacg atataacgtt cgatcgtgcg tcggattgtt gccccttttg gaagccagaa
     2221 tggcaaacca gaacctactt ctggggaaac catgaacaag tcaagttctt ttcctagttt
     2281 acgatggtct ctttcttttg cttcttcacg gagacggata tattctttca aagctttctt
     2341 gtcaaaaaag gctgtcccgt atacacgttg catcatttga ttgtttgagt ttccacgcca
     2401 ataagcacct gccactgata ataatttaaa tacttgtata cggccagtgg atggaacatg
     2461 tggtccacga cacaaatcga tgaattcgcc ttgttgataa gcagtaatca cttcttcttc
     2521 tggcaattca ctaatcagtt ctactttgta cggatcactt gcaaaaattt ctaatgcttc
     2581 ctgttttgaa agaacacggc gttcgatcgg cagattttct ttgacgattt tcatcatttc
     2641 tgcttcgatt gccggcaaat cttctgcagt tacttggttt tgtccattat ctgtatcgta
     2701 gtaaaaccct gaatcgatag ctggtccaac gccaaagtgg atatttggat agaggcgacg
     2761 catggcttgg gccatcaaat gagctgctga atgacggact aagccaagtg catcttcatg
     2821 gtctggtgtg atgatctcaa tacttgcatc ttcctcgatt ggacgagtca aatcaacgag
     2881 ttttccgttc actttaccag ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc
     2941 aattgccaat gttgttgttc cagactcaaa ttctttgact gcaccatctg gaaaagtaat
     3001 ttttatcata atagtttcct ccttgaaatg ttttgattga tcagatcatc aagctgaaaa
     3061 acaaaaaaat cctagcatct cttgaaaaga gatactagga cgaaaagttc gtggttccac
     3121 ctaatgttca aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc
     3181 aacaagggtt tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt cagcctagct
     3241 tttcctctct tatcgaagac tctcaaattc agttggcttt ctcatcaatc aattatgtgt
     3301 ttatttaacc actaataaat agaaaaatca agtgggaaac taaacttatt ttttccattc
     3361 cccatttaca ccgtatgttc cttttttcat ctcattgatg atgacatgga tgtgttcttt
     3421 tggtgcctgc gtgtttttat gaacagcttc cgtgatatct ttcatcatat tttctaattg
     3481 ttccggactg cgaccttcta ctaattcgac atgtacgaat ggcataatgc tctctcctct
     3541 ttctgatcgt tcttttttca actcgattct atcatactat gtttgtcttg ggtactgaat
     3601 caggaaaaat ggctggatct ttttttgctc tcaaggcact caaaatagag accgtatcca
     3661 cgacttcttg caaaacagct ccgaataatg cggggattag tcccgtactt gcaattaaca
     3721 tcaaaaaaat acaaataaag atgccaatca gtacagactg tttcgctatt ctcatcgtat
     3781 ctcttgaaat cactactgct tttgctacac gcaagagatc atctttcaag atgaccgcat
     3841 ctgcactttc actagcagct gtagaaccat gagcccccat tgcaataccg atatctgcag
     3901 cagcaagtgc tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt
     3961 tagggatact ttctaaaatg gtcagtttat cttgaggcaa gctacgggca tgtacagaat
     4021 ctaattggac tttttctgca atcgtttgag caatggcaga tccatccccg gtgatcatca
     4081 tcaaattttt cacatcaagt tttcttagct ccttgattgt ctttgctgct tctgggcgaa
     4141 tctgatccga aaataggatc gttcctgcaa aagatcgatc aattgttaca tagattgctg
     4201 tattggacgt ttctatttct tcagctgaaa caaaagaggc acgtccaatc tttacttcat
     4261 gattattgac tactgcttgg attccttgac cggtcacttc tttcagttga ctaacaggat
     4321 acagttggct gtttttcacg ttgttcacta acgaacgagc taagatatgg ctagattcct
     4381 gttctgcact agctgcatac agaagtagct cttctttttt tattccattg actggctgaa
     4441 tcgcctctac ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt
     4501 ttgtacgtgc gagcttttca atcgttgttc cagtttttac aacgatcccg tttttacttg
     4561 aacggctcat tcctgcaact aaagcaatgg gcgcagctag aatcagtgga caaggtgatg
     4621 ccactaccag tacctcagca aatcgtaaag ggtcttttga gaaccaccaa gaaataccac
     4681 cgatcaaata agcaatcaac gtgaaaggga ctgcataacg atcagctaaa cggacaaatc
     4741 gggcaggctt ttcttttgat tcttcgacta gtttaataat catttgatac tggctgtctg
     4801 cggcacgttt ttcagcgatc atttggatcg atccgtctcc atttaaagac cctgacataa
     4861 tctgatctcc aggtttcttg gtttccgggc gggactcacc tgtcaaagaa gattcatcga
     4921 ccgaagattc tcctttggtc actcgaccat ctgcgggtac aagttcgcca ggcttgacta
     4981 gtaactggtc atcaatattt atttcatcga ttactatatc ctgcaatttt ccttctttga
     5041 atcgatgggc aattcttggg gtgttatcta acaaagactg cagttcttta cccgctcgtt
     5101 tattcgcgta atcctctaga ctatctccgc ccgtaagcat gatcaatatc atcaaactag
     5161 cccagtattc tttgacggct aatgttgcaa ggattgcggt aatcgctaat acatctacgc
     5221 catatcttcc tgaacgtaac gtcttgatca tttccaaaaa catcaagaag gcgagaatcc
     5281 ctccagtaaa tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca aattcactta
     5341 ataaagctat cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt
     5401 tttctcctct tttatgtgac ttagagaggt tagtaataaa tttccagctg cctctttcat
     5461 cttaagcata gtccttgact agcacttttt aaaacaatag gccttaattg aaaacaacta
     5521 tcactatcag cgtttccaaa attcatcagt tctacaaaca aaaatattca gcaaggatta
     5581 ctttttcgat tcatcagtta aaaagtacct tttttcgaaa aactatgcta tacttttaat
     5641 tcgttattta cggcggaagg agcaaacatg cgatgaatga gaaacaacta gaacaaacgc
     5701 acgtggatga aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat acaaaacaac
     5761 aagaattgac tcaggaaatg aatcattcga caaaagacac agctaatcac aagattcgag
     5821 ctggttccaa tgaagctttt tatgaatctg ctgttgaata tcgccaacat gagcaggaat
     5881 tgcttttgaa ataccatact cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca
     5941 tgaaagatag tccttatttt gcccgtatcg actttaaaga agaccgtgaa acagaaacat
     6001 tatatctagg tatcgcttct ttaagagatc tggcagaaga aacaatcgtt attgactggc
     6061 gcgcaccaat tgccaatctt tattatgaag gtgagattgg tcccgcattt tatgaaactg
     6121 aaacagatag aatagatgtc gatctgcagt tgaaacgaca atttaaaatc caagaaggac
     6181 gtattttatc tatggtagat acgtcggaag taatcaatga tgactttttg ctagagatat
     6241 tggatgaatc atcaagcaac catatgaaaa acatcgtctc tacgatacaa aaagcacaaa
     6301 atgcaatcat tcgggacaca acaagtaaag taatgctgat tgaaggaatc gccggaagtg
     6361 gaaagacatc cgctttgctt caacgtattg cttttctgtt gtatcataat cgcaagtggc
     6421 tcgaggctga aaacgttctt ctattttcac ccaaccattt gttttctgat tacatctcga
     6481 ctgtattgcc ttcattaggt gaaagcggcg tgcctacaca gacattcaaa aattatttgg
     6541 tgcagctgct gccagagttt tctttactag atgaacagca acaagaaagt ggttttttgc
     6601 taggggaaaa agatccgata caagtgatga aaagcggatt gaccttagtc gatcagattg
     6661 atcgctatat ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg aaaataaatg
     6721 gccgtacgat cctatcaaag gaatcgattc gccaatggta taaagaaaca aatgaacaat
     6781 tacctttaca tcatagactt tctctattac aaacgaagct cctgaaaaaa ttaggcggtc
     6841 tgcaaaaaga cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa cagctccaag
     6901 aactatacgc aacagatcca aatcttgaat atacagaaca aaaggagcgt tcattacgta
     6961 aaaaactggc caaccaaatc gtacgtaaaa gattccgtcg aatccatcga gggatcatga
     7021 attatcaatt tgtcaatata acgaaacaat atctccactt tctacaaact gtgccacttt
     7081 ctcttctaac acgacataaa atcagcgaga ccaggtggca ggaacatttg acaactgttc
     7141 gtacacactt gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc tttttactga
     7201 tgcgccgaac atatccagtg acggttacac acaaagctcg ctatatcttt attgatgaaa
     7261 tgcaggattt cgcaccatca caagtggcat tgctgcaagc cttgttccca aaagcaaatt
     7321 tgactttctg tggagatcta aatcaaaatg tctttggaaa tgaaacgatc gttggatcat
     7381 tggaagacct tttcgtggac cgtgaagtca ctcgtttcca attaacgaca agctatcgct
     7441 ccacaaaaga aatcactgat tttgctaatc attttattgt gaaagaacat caagtagaaa
     7501 caacagcaag aaaaggagac cttcctttgc tcatccaagg aacaacagat gatatcaaat
     7561 tgaattggtt agcagatacc attcgaacga ctcagggaaa agcccggtat tggcgtacag
     7621 caatcatagc taaaacacaa gaagaatgcg aagctcttta tgctcaactg ccagacgagt
     7681 tgaaacaaca agtccagctg attttaaatg agagtgactt tatgaagcgt tccattattt
     7741 tacttccagc ctttttagca aaaggtctgg agtttgaccg agttttcttg tgggatgtag
     7801 atgcagaaca tttttcttct cagcaagaca aattgatcct ttacaccatg tgtacacgtg
     7861 cgatgcatga attgaccctt tttacctcca ataatgaaag tcctttgtta aacaatatgg
     7921 atatttcatc ttatgagaaa aaagtactgt aagtaaaaag acaactgcta tcattaatgt
     7981 tagcagttgt ctttttattt acaataataa ttgcagaaaa agtaattcaa tcatttaagg
     8041 tcatttattc tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa
     8101 taatcatttt cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac
     8161 ggccaatccc gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa
     8221 ttgattggaa attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg
     8281 ctaggtatgc actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga
     8341 taagaatttt ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca
     8401 tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa
     8461 tcagataatt ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac
     8521 ggttcagata ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg
     8581 cgtatttttg cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc
     8641 ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc
     8701 ccgtccatct tccacatact tcatatcatt tcacatgata cttatgctct catcatacca
     8761 caggttgaat tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga
     8821 ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct
     8881 aatgccacat tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt
     8941 ttttcaaatg tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg
     9001 atctcagcga ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg
     9061 aaatccgcta cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca
     9121 tcgatgtctt tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca
     9181 aagattgctg tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca
     9241 tttttcccaa atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta
     9301 tcaacaatca ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt
     9361 tcatctcctg caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta
     9421 taagttttag cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca
     9481 cctgattttg ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta
     9541 gcatctttag cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt
     9601 cgctttctga tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat
     9661 cacgattctg tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat
     9721 ctatactcgg attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata
     9781 gatttgatca cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga
     9841 ataaatagaa atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag
     9901 tagtcgaaga taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa
     9961 gacataacaa gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga
    10021 cacaaaatcc tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc
    10081 ttcggtcatc tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg
    10141 caaatacggc caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt
    10201 ctgaggcggc acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt
    10261 tgcactggct atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg
    10321 gatcggatgt aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc
    10381 caagatatga tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac
    10441 ctcagtcatt ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc
    10501 tatcctatct tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc
    10561 ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc
    10621 tttagcaaaa tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc
    10681 cacaccattt tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc
    10741 tctttgcaaa aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg
    10801 tgccatttcc acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc
    10861 gcagcttttc atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta
    10921 aaaaaaggca aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat
    10981 acttctgcga cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt
    11041 tatgttgaag atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta
    11101 tgctacaaag aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa
    11161 tgcttcgact ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata
    11221 ggtacgctct tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc
    11281 aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca
    11341 caaaaaagca aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac
    11401 ttgataggtg gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac
    11461 ttcatcaaac acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa
    11521 acaggtaatt gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa
    11581 ttggatcgat gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga
    11641 aaggtctaac cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt
    11701 accgcatgcc agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc
    11761 taaccattgc tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc
    11821 ataagccata tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag
    11881 tttttccaga ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc
    11941 aagatcgatc aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt
    12001 gttttcttct tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt
    12061 tgctgaacag atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat
    12121 aatgtcttct gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact
    12181 atctatgatg atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat
    12241 gtttcgattg tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt
    12301 tttgtttcaa atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt
    12361 accccaggaa aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg
    12421 agactcatct tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg
    12481 gtgattccta attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta
    12541 ttataattca ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca
    12601 tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa
    12661 gcaccgtatt ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag
    12721 cgttgttcgc tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta
    12781 tattcattac tatagtccat attcatacaa cccttcattt tgaatataat caattacttt
    12841 atctggaaca aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga
    12901 actgatgtcg atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta
    12961 tcctggtcga cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca
    13021 ttttggcaaa tattctacca tatccccacc gataataaag taataatctg tatccggatt
    13081 gttttgcgtc aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc
    13141 aatcgtttca atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg
    13201 atgttttgca tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa
    13261 aaatacgcgg tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag
    13321 gtgaacggga ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg
    13381 aggcatttct tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat
    13441 ggtcactcct tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga
    13501 aggtttgtat aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt
    13561 ttctaatact tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt
    13621 gatcagttct cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc
    13681 accttttccg atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt
    13741 ttgttttcct cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa
    13801 tacatctaca ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca
    13861 gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag
    13921 ctccggaaat gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt
    13981 ttgataaaat tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac
    14041 ataagcaata aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa
    14101 gaaaagtgtt tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc
    14161 aatgatccgc aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc
    14221 cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt
    14281 tcctggaaac tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg
    14341 attgataagc gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc
    14401 gcggtattct tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt
    14461 taataataca tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt
    14521 taatttagtt tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac
    14581 tggattgtca ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat
    14641 atccaccaca ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa
    14701 gtcgtcatcc gtcaatgaga catcttgggt ctcattataa tggcgtaatc tgaaacaacg
    14761 ctgacaatag acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc
    14821 taattcaccg ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc
    14881 tgtcattcga ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata
    14941 attctttttt cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt
    15001 tttacaagga tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga
    15061 tcgcctacca ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc
    15121 cctcttgcta aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct
    15181 cgcgcgacac gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc
    15241 atttctaaaa tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac
    15301 gtattgtcta aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta
    15361 atctgataga tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct
    15421 catctgcaaa aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat
    15481 cagcaaaaaa aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa
    15541 tgaccaaaaa taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc
    15601 ggaacctctt gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca
    15661 atctttcaat caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc
    15721 cgttgaacgt ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag
    15781 taattttcat caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg
    15841 caaatccttc tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg
    15901 ctaaacctag agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg
    15961 gtacctttaa atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct
    16021 cttctgctcc tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat
    16081 tgaacttttc cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag
    16141 aaccaaaatt tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg
    16201 ttaccggttt gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata
    16261 aacgatcacc atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg
    16321 tccatcgttc tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct
    16381 atccatcctt tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg
    16441 cggggaacaa tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct
    16501 tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag
    16561 ctttggggtt ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg
    16621 gtcgtcgggt ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca
    16681 gcagcagaga tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca
    16741 cctgaagctg tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg
    16801 gtgatctttt ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact
    16861 ccaataatga agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt
    16921 cctgttttat cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct
    16981 ttggttacaa gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga
    17041 cgctgccatg caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg
    17101 atgtctttcg tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga
    17161 acagatggcg agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt
    17221 ttcttaaaca gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa
    17281 aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc
    17341 aaatccatct gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct
    17401 cgctgcatct tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc
    17461 attgaatcat aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt
    17521 cccatcccac ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg
    17581 aaagcaggat tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct
    17641 tcttgacaca cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg
    17701 attcctgtga ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg
    17761 aaataaaaat cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg
    17821 actgctttag ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc
    17881 actacagaag gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc
    17941 tcgcctaaat gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga
    18001 gcaggataaa tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct
    18061 tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct
    18121 ttcccgccac cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt
    18181 tccaatgctt cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt
    18241 gcttcttgca tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt
    18301 ttaggaccaa taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca
    18361 gataaaaaac caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt
    18421 acttcctgaa cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca
    18481 tctgctaact gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg
    18541 atccctagtt cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc
    18601 aatactttag aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac
    18661 ttttttaccg tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa
    18721 tatctcaact tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt
    18781 gtccaatccg ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac
    18841 tgctcctgct tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg
    18901 aaaatgtcct tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc
    18961 tccttcaacc atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat
    19021 ttcttttatt tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg
    19081 aatcctaaag agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt
    19141 gatcactccg tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca
    19201 gataacttct cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc
    19261 tggttgcaga taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc
    19321 cgattctttt tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac
    19381 tggctccgaa actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt
    19441 atttttattc atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga
    19501 ttgatcaaat tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag
    19561 cgtttcatgc acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa
    19621 gtaaaacttg caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt
    19681 ttttctacat tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag
    19741 gcttcgatcc cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg
    19801 taagtatctc tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca
    19861 ttggctggtg tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca
    19921 gcttcttcca tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct
    19981 gtcatatggt aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca
    20041 ccgcgagctt gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc
    20101 acaaaaccat tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct
    20161 gagagtgctg ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt
    20221 cctccagcaa gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt
    20281 gaattcgttc cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca
    20341 cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa
    20401 actcgttttg cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca
    20461 ccgatcccag atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa
    20521 ccgctcatct ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt
    20581 cgtttgctat cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct
    20641 aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc
    20701 tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag
    20761 ccagtaatta ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat
    20821 ccgccatcga cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct
    20881 gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt
    20941 tccttgactt tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg
    21001 gcattacatg taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact
    21061 acgccagctt tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca
    21121 ctagagagat taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc
    21181 gtcatattga aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc
    21241 attcgcatca caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat
    21301 ccaagctttt cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca
    21361 ccggaaactc ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga
    21421 atctctccgc gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc
    21481 gctttgccga ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata
    21541 acgttcctcc ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca
    21601 gaactggtat tgttttatcg atttttttca taaatccgct taatactttt ccggggccga
    21661 cttcgatgac ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa
    21721 caggttgcat gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg
    21781 ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct
    21841 tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta
    21901 gagggatcat tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt
    21961 tttcttcgcc accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc
    22021 cgtacttgct agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg
    22081 caaccatttt cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta
    22141 ctaacgcaac tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc
    22201 ctaaactcag tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat
    22261 acgcgatgct gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat
    22321 tttctgtaaa gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg
    22381 tctgctttac aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt
    22441 ggccactaaa taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt
    22501 gattcttcct attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa
    22561 cctaatttta atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt
    22621 ttgattccgt cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt
    22681 tggaataaat ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt
    22741 tcttcagttt cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata
    22801 ataatatatt cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc
    22861 catgagaata cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg
    22921 ttttcctaag gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc
    22981 agcagatgta ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac
    23041 ttttcgggca attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac
    23101 tggtttgtct ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa
    23161 aatatctctg ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg
    23221 atttttattt tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa
    23281 tttttctttc agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc
    23341 gccaaacaac acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc
    23401 accaatcacc agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc
    23461 ataaacaaat cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa
    23521 tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac
    23581 taaaataaaa tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac
    23641 taaaatgcat aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt
    23701 ccttgacgaa atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat
    23761 aatcttttca ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata
    23821 tcctcactta tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg
    23881 ctgttcctcc gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg
    23941 atggacccga aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc
    24001 atcttcactg cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt
    24061 caaggtccca acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta
    24121 catcccgatt gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt
    24181 tttcagctca gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt
    24241 tactgttttt aaatctggtt ccatgttcca ccgtccacta attagtttga ttatcaaatc
    24301 ttttgatgtt caaactatat cttaactttt ttcaaaatgc aaccttaaaa gatcattttt
    24361 ttactatatg agcttttttt aagattttac tcatttaaaa caactgataa atcagcattt
    24421 ataatttctt ttattagaaa aatcatctat ctcacatatt ttgattatca aagtattttc
    24481 tgtgaacaga actatagaat tccctaaaac ttgtccatta tttcaaaatt tgttatagtc
    24541 tttttattca aagcgtaaaa ggagatccta tgttaaaaga aaaaatgaaa actctcccca
    24601 tgctgccggg tgtctatctt atgaaagacc gagatggtaa ggtcatctat gttggtaaag
    24661 caaaacggct caaaaataga gtaagcagct attttcatca gaataaacag cattcaaaaa
    24721 aagtattacg tatgattcat catataacag attttgattt cgtcgtagta gacacggaat
    24781 tagatgcttt attgctcgag tgtcaattga tccaacatta ccgtcctttt tataaccgac
    24841 aaatgaatta tttcagtaat tataattatg tacatattac caataaggga tttgtattaa
    24901 cagatactcc tacggctcga acatacgggc cttttcgtct ctataaaaaa atgccgtcta
    24961 ttttgcgtat aatggaagaa acttatcaaa tgccttggtt gtctgaaatt agtttgttag
    25021 ctttgcgagt ccagcttcct gacctacagg aaatgacttt tgaacagaaa aaaaaggaac
    25081 tccaagggct gtttcaaggc cgaaataaaa agctactgac ttacttgaaa aaaagacaac
    25141 aacatttcat ctatcagttg aatttcgaaa aagccggtat gcttcaaaaa gatatcgaat
    25201 tagttactta ttttattaga cggatccagg aacaaaagca gtttttaaga acaccatcgc
    25261 tcaccttcag catgccatta gcagcagatg agagtcagaa aaagcactac ctcgtttgtt
    25321 atggtcaact tgcagaaaca atgattacct ctggtgacgt ttctcctgac ttctattatg
    25381 aaaagaaaga agctcattta tctttgaaac gtcaattgtc aaaagaagaa atcgatcctg
    25441 tacaaatctt gatcagttac cgcaaaaaat tagaaaaaga acagtttgaa attgaactgt
    25501 taaacaaaaa agaagctgaa aagcaactaa attagttgct tctcggcttc ttttaggcat
    25561 cttcaacttc aaatgtcaat tatcctaaaa tttattggat taagtcatag atttccatcg
    25621 ctacgatatc gatattatca aattcgtatt gctgatgagt atctgtttca gttaattcaa
    25681 atgtatcggt cgctttatca taagttactg tacacttctc tacgccttct ttttcaaaac
    25741 ggcgaacttg cagttcatta tctgctgact cgaccatagc ctccagtcgt tttatgattg
    25801 cgactaattg tgaatgtgtc atcggaggtc ctccttttct tatttctgtt atattgtatc
    25861 aaaagcatag cctacttgca ttgattttct ctaaaaaaac tcgtttttta tgattttttt
    25921 tctggtattt ttccttcagg ccaccaaagt ttgatcaaag cttgggttcc gtcattctca
    25981 aaacctttat ctgccaactc ttcatatagt ttcaatgctt gggtagttgc tggcaataca
    26041 agatccattt tttctgcttc ttctaatgca atctttaaat ctttgataaa atgtttgaca
    26101 aaaaaaccag gtgtgtaatc ttcttttaaa atacgaggag aataattgct taaagaccag
    26161 ttagctgcac ttccacctga aagcgtctca atcactttct ttaaatcaag tccgtttttt
    26221 tgtgcataga caagcatttc cgtcattcct gtcatcgtac ctgcaatcat gatctgatta
    26281 gccatttttg tatgctgacc tttcccagca gaaccatgca atgtaaatgt cgtaccaaat
    26341 tctttgaata aaggcagtac ttgatcataa acggcttctt ctccaccgac catgatcgtc
    26401 agtgtaccat tctttgctcc taagtcacca ccggataccg gtgcatctaa cgcatctgcg
    26461 cctttttctt ttgctgtttt tgcaattttt tctgccaggc ttggtgtact tgtagtcaaa
    26521 tcaactaaaa tatgtcctga gatatctgca gaaaaaatcc ccgactgtcc atagtagatt
    26581 tcttctacat ctttggggta acctaccatt gtaaagagaa tattactttg ttctgctacc
    26641 gcttttggtg tatctgccca gatagctcct tctgttacta aaggatcagc ttttgctttt
    26701 gtccgattat agacagctac ttcgtgtcct gcttctagta gatggcgtgc gacagcactt
    26761 cccatgacgc ctgttccgat aaaaccaagt ttcatttttt cgacctccta aaaatcaaaa
    26821 atccttgctt ctatgctagt atacgctaga aacaaggatt ttttcataaa aaatgtttga
    26881 attttatctt ttaaatgaat ttaattttat tttgattcgt tcaatcgagt cttcttcttc
    26941 ttttcttcgc atctcttctt catgataaac aaccatgtct tgataaaatt cgatcatagc
    27001 ttcgccgaaa tgtgtagatg aaatatcata aagtttagct tctaaaactt ttggatcttt
    27061 aggaacttca ctggaaatat aatcaataag tgctggtgca aagtcttcgt cggtttcaaa
    27121 agttttccct aatgaaggat gatcgaacaa tcggttcaaa taagcatttc cttccacgac
    27181 acaaggtgtt ccggctgcca tcgcttctgt ataagtaagc ccttgtgttt ctgaagtaga
    27241 agcactgaca aaataatctg ccgctttata ataaagtgct acatcattgt tgtccacttc
    27301 tcctgtgaac tgcacaaatt cttctaactg cagttctgag atcaattcct ctaactcttc
    27361 tacataaggt ccttttccta cgataactaa tctggcgtta ggataagcgg agatgacctg
    27421 aggaaaccca ttaatcaagg cttggatatt tttctcataa gaaatcctgc ttaaagaaag
    27481 aatcatcaaa tggttttcct gaagacctag tgactccctt aatttgctga tatcctgctg
    27541 agtgatatca gaacgtttga atttttcaat gtcgatacca gtaggaatca cgcgaagcgg
    27601 tgctattaca ccatagctgc gtaatgtatc aataacacgt tcacttggac aaactacacc
    27661 tgtcgtatga tttgcaaata atctagacaa gtattttaca tgggattgcc tgatcacttt
    27721 tcctttaccg atataatgaa ggtagtcttc atacatcgta tggtaagtat ggatcagagg
    27781 tattttcaac ttcttagcta ccatcttccc aagtatccct gctccgaatt ctgtatgggt
    27841 atgaataaga tctagttcta gttctttggc aaccaaataa gcataccaca tgcctctaac
    27901 aacaactctt cgatccttga aagaaataaa tggtacactt ggcattcgaa tcacatcttt
    27961 ttccaactta tccgcattag gatcggtcgt tgtaaaaata tagacttcat gcccatgtct
    28021 ttctaattct tgttttaatg ttttgatcga tgtagcaact ccgctgactt gcggaaagta
    28081 ggtatcggta aaaaagccga ttttcatgtt tttcgcctcc aaactagtcc ttgacctgat
    28141 ttactttttt taatttgcta gagacttttt cattcagtac actttcgtat acatttttaa
    28201 gttcttcacc gattttattg atacttcgct cttttgctgt ttgataaccg gcttgtgtcg
    28261 tactcggtag ttcttgattg gtaatctgtt ggatcagtcg gataaattct tcgtttgtct
    28321 tccccatata gcagttcttg ctatcttcta accagccatt atacactgga atatctcgaa
    28381 caagtacgtt ttgtttgctt gctaaagctt ctaaaacaac gatcccttct gtctcttcat
    28441 aagaagggaa aaagaataga tctgcacctg aatacgcacc ttctatgatc tctccacgaa
    28501 tgtaaccagg aaacaggaca ttttcaggat gatcttcttt tacaattttc cggatgttcc
    28561 gaggaatcga gtacatggga acatgaccaa accaaataaa tgtgtattcg ggcatcttct
    28621 tagcaatttc tacgaaatca atgattcctt tacgttcgaa aaacaaaccg acacaaatga
    28681 ttactttttg atccggagac agtttgaaat attctctgaa cttttgttct ttcattggat
    28741 cagggtgata tttctctaag tcgatcccat tggaaatagc ttgaatcggc acacggatac
    28801 catagctttc caacaactgt tttgaataag gtgttggcgt gatcagatag tccgcttttt
    28861 ggtacaatcc taccagatat ttttttacga taggagaaag ctggttcgat ccgataaagg
    28921 aatttcggaa atcttcctct gtagaatgcg catgatagac aacttttttt ccttctcttc
    28981 tggctttatt gaccatatta tgactattga ttccgtatgt attgatgtgc aggatgtcat
    29041 aatcttcttt ttcatctagt gtataagaga taccgacttc cgtcagagca cgtttttgat
    29101 gatcaagcgc acgcccgatt ccagattttg caagcatctt ttcactttca aaatataata
    29161 ataccttcac cttacgtccc ccttcactta tatagtataa catagcctac aaactgtcac
    29221 tcaaacttaa gccctatttt tgtctattct tactgacaaa aacgttttct taaaaaagaa
    29281 caaaaaaact ggcgtagctc ttttacaagc cacaccagct tttgatacgt tgtttatttt
    29341 acatattcat gaacaagttc aacgacttct tccatcgtat cgcattcttt caatgcacga
    29401 tcagcaagtt cagccatttt cgctgtgtcc aagcgtttca ttaaactacg cgttttaaga
    29461 atagatgtcg cactcattga gaactcatct agacccatac ctactagcaa tggaacagct
    29521 gtttgatctc cagccatttc tccacacata cctgcccatt ttccttcagc gtgtgctgca
    29581 tcaattacat ttttgatcaa acgtaaaatt gatgggttat atggttggta caagtaagaa
    29641 acgcgttcgt tcatacggtc tgctgccatt gtgtattgaa tcaaatcgtt cgtacctaca
    29701 gagaagaaat ctacttcttt tgcaaacttg tctgctaaga ctgctgctgc tggaatctcg
    29761 atcatgatgc cgacttggat cgtatcagat acttccttgc cttcacttat caatttttgt
    29821 ttttcttctt caaagatcgc ttttgctgcg cggaattctt tgagtgtagc taccataggg
    29881 aacatgatac gtaaattacc atgaacagat gcacgaagca atgcacgcat ttgtgtacgg
    29941 aacatttcgt cgccttgttc agataaactg atacgcaatg cacgatatcc taagaatgga
    30001 ttcatttcat gaggtaattg cagatatggt agttctttat ctccaccgat gtccattgta
    30061 cgaacaacta caggttttcc ttccatacct tctaatacag ctttgtaagc ttcatattgg
    30121 tcatcttctg ttgggaagtc tggagaatcc atatacagga attcagtacg gtacagacca
    30181 actgcttctc caccattatt atggacacca actagatctt ttggtgttcc gatgtttgca
    30241 gctaattcaa aatgcttgcc gtcagctgtg acagtttctg cattttttaa tttttcccat
    30301 tctgcttttt gtgctgcgta atctgcacct gctttttcaa attcagcttt ttgttcatct
    30361 gtaggatcga tgattacatc gccttcgatc ccgttgacag ccaaaatatc gccttctttg
    30421 actttagcag taatttcttt tgtcccaacg attgctggaa tttctaaaga acgtgccatg
    30481 atagcagaat gtgaagtacg tccaccaatg tctgttacaa acgcttttac aaaattgcga
    30541 tctaattgtg ctgtatcact tggagtcaag tcatgtgcaa caacgaccac ttcttcgttg
    30601 atcattgatg gatttggtaa agtaacgcct aataagtgcg ctaaaatacg tttagcaacg
    30661 tcacgaatat ctgcagcacg ctcttgcata taagcattat cttccattgc ttcgaacata
    30721 ccgatataca tatccgttac ttcttttagc gctgattcag catttacgct gttgtcttta
    30781 atattttgct tgatttgacc taccatttct ggatctgaaa gaaccatcaa atgggcatca
    30841 aatacttgcg cttctgcttc acctaagctt tgcgctgctt tttcacgaat ttgctgcaat
    30901 tcttcagtag atttagctaa tgcagcatcc aaacgagctt cttcagccgc agtatcttct
    30961 actgttgcct tactgaatgt taaatccggt tgaactagca ggtaagcttt tgcaacggct
    31021 acaccgtcac tagcggcaat cccttttagc atttcaacca ttattcagat aagccttctt
    31081 tcttcattgt ttcaacgatt gctgccattg catcagcttc gtcagcacct tctgcagtaa
    31141 tagtaacatc agaaccttga cctacaccta aagacataac gcccatgatt gatttcaagt
    31201 ttactgattt acctttatac tctaagttta catcagagtt aaatttgctt gctgtttgta
    31261 ctaatagtgt agctggacga gcgtggatcc cagtttctgc tactacgtga aattctttct
    31321 tttccatatt aatcggtctc ctttaagtga gtgttgtttt tatgtgaggg ttttcttttt
    31381 tagtaattca gagaatccta acaaatgcca ccctttcatc tgtaaagatt aacatttttt
    31441 ttcgttagat acaacagttt tcattcaatt tcagataaaa gtattcaaat tattccggat
    31501 tctttttcat ccttttcttc acattttata acaaacgtgt ttatttcctt tttcttgtcg
    31561 ctttctcttc tcctgacgtt tatcattctt tcctttgacc ctctttgttt tcatatgaaa
    31621 cgaattactt gcttcccttt ttatttttag tataataaga gtcaaaggtc aggaatggtc
    31681 aatagagatt tcaccttttc tacctcataa atctcttaaa aaactagggg ttattctttt
    31741 acctcatgaa aaacgagatg taaaatatgg atgtacccat ttttgtgaaa ggacgtgaat
    31801 gattcatgct ttgtcaaaac tgcggcaaga atgaggcaac gattcacttg tatgccactg
    31861 tcaatggtca gcggacacaa ctcgattact gtcagagctg ttatcaaaaa atcaaaaatc
    31921 aacaaaatgg aggtctgact aatatggccc aaaatgatcc atttggattt ggcagtttgg
    31981 acgatctttt ccgttccatg tctcgccaaa tgcaacagca aggaaattat gagcaaacac
    32041 caccaacaca atttggcgga aacaacaact tcaatggtgg tcagccacct caaggcaata
    32101 cagaaggttt attgggagaa tacggaatca atatcacaga acaagcacga caaggtgata
    32161 tcgatcctgt gatcggacgc gatgacgaaa tcaaacgagt cattgaaatt ttaaatcgcc
    32221 gaaccaagaa caatcctgtc ttgattggag aaccaggtgt tggtaaaaca gcagttgttg
    32281 aaggtttagc acaaaaaatc gtagatggtg atgtaccgca aaaattaatg gacaaagaag
    32341 tcattcgttt agatgtcgta tctttagtcc aaggtactgg tatacgagga caatttgaag
    32401 aacgtatgca aaaattgatt gaagagatcc gtcaagcaga aaatgtgatc ctattcatag
    32461 atgaagttca cgaaatagtc ggtgctggtt ctgctggtga tggaaatatg gatgctggta
    32521 atattttaaa accagcttta gctcgaggag aattgcaaat ggttggtgct actaccttaa
    32581 atgagtaccg catcatcgaa aaagacgctg ctttagaacg tcgtatgcaa cctgtacggg
    32641 tagatgaacc aactgtcgaa gaaacgatca gtattttgaa aggtttgcaa aaacgttatg
    32701 aagactatca tcatgtaaaa tatactgatg aggccatcga agcagccgca actttatcca
    32761 atcgttacgt ccaagatcgc ttcttgccgg ataaagccat cgatttgcta gatgaaacag
    32821 gttctaagaa aaacttgacg atccaaatcg ttgatcctaa aacaatcgag aaaaaactgc
    32881 aagaagcaga agaacaaaaa gttttagctt ctcgagaaga agatttcgaa aaagcagctt
    32941 attatcgcga tcagatcaat aaacttcaaa aaatgaaaga acgtcagctc actgaagagg
    33001 aaacacctgt gattaccgaa aaagatatgg aaaaaatcgt tgaacaacgg acaggtattc
    33061 cagtgggtga attaaaagaa aaagaacaga cacaactgaa aaacttagca gatgacttga
    33121 aagctcatgt catcggacaa gataacgctg tagatcgagt agcgaaagcg attcgccgaa
    33181 accgtgttgg attaaacaaa cagaatcgtc caatcggctc cttcttattt gttggaccaa
    33241 ctggtgtagg taaaacagag cttgcgaaac aacttgccta cgaactattc ggttcacaag
    33301 attcaatgat tcgtttcgat atgtctgaat atatggaaaa acatagtgtc tctaaattga
    33361 ttggttctcc tccaggttat gtcggatacg aagaagctgg tcaattaaca gaaaaagtac
    33421 ggagaaatcc ttacagtctt gtcttgttag atgaagtaga aaaagctcat ccagatgttt
    33481 tgcatatgtt cttgcaaatc ttagatgatg gtcgattgac agatgcacaa ggaagaacgg
    33541 tcagctttaa agatacgatc atcattatga caagtaatgc cggaactggt aaagtagaag
    33601 caaacgttgg ttttggtgcg gcacgcgaag gtgttactcg ttctgtattg aaccaattaa
    33661 acaactactt tacaccagaa tttctgaacc gttttgacgg gatcatcgaa ttcagtgcat
    33721 tatcaaaaga aaacttgatg acaatcgtca cactaatgct agacgatgtc aaccaaatgc
    33781 tggcagcaca gcaacttcac atcgaagttc ctacgaatgt caaagaaaaa ttagttgact
    33841 taggctatga tccatctatg ggtgctcgtc ctcttcgacg cacgatccaa gaacaaatcg
    33901 aagatggtat cgctgaattt tatcttgatc acccttctat ccatgagttg aaagcaaaac
    33961 tggataaaga tggaaaaatc atcgttactt ctaaaccaga acgtttggct aaagaatcag
    34021 ccgaagaaac ggctgaataa atccagtttt atagcgtgag acggtatttc tttgagaaat
    34081 gccgtctcat tcttttttca gaaaagtcaa aatactgttc attttataaa aaagaaatta
    34141 taataagaga aacaagcaat taaaaataca taaaagaggt gagcgccatg aaattggtaa
    34201 atgtaacaaa cagccattcc cgtcttgtat taaaccagct tgaaaacact gatgcccatc
    34261 tagtcaaggt ctacacagcc ggaaatacca ctattgtcta cactgaagcc cctcaacata
    34321 atgaaattct gctgatcaat gacaaacgta aaatccagcc gaaagaaatc gaagatgcta
    34381 aaaattattt cttagataaa atcaaagacg ccgtttacca cgaggatgaa atcaaagtaa
    34441 tcgaattggc aggccttgtt gaaatctcga ttccaaaaca tgaattaggc gctacattag
    34501 ccgtgtaaac aaattttgaa cataataatt aaagagcgtg ggacaaaagt aaa
//
LOCUS       DAFCHQ010000021        32552 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000021 DAFCHQ010000000
VERSION     DAFCHQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.021"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            363..731
                     /locus_tag="LNC71_001096"
     CDS             363..731
                     /locus_tag="LNC71_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBK6466182.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="LNC71_001097"
     CDS             911..1852
                     /locus_tag="LNC71_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBK6466183.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="LNC71_001098"
     CDS             1921..2652
                     /locus_tag="LNC71_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBK6466184.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="LNC71_001099"
     CDS             2796..4976
                     /locus_tag="LNC71_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBK6466185.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="LNC71_001100"
     CDS             4996..5478
                     /locus_tag="LNC71_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBK6466186.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="LNC71_001101"
     CDS             5494..6255
                     /locus_tag="LNC71_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK6466187.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="LNC71_001102"
     CDS             6386..7720
                     /locus_tag="LNC71_001102"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBK6466188.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="LNC71_001103"
     CDS             8027..8512
                     /locus_tag="LNC71_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBK6466189.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="LNC71_001104"
     CDS             8505..9188
                     /locus_tag="LNC71_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBK6466190.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="LNC71_001105"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="LNC71_001105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBK6466191.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="LNC71_001106"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="LNC71_001106"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBK6466192.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="LNC71_001107"
     CDS             12804..13433
                     /locus_tag="LNC71_001107"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBK6466193.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="LNC71_001108"
     CDS             13485..13817
                     /locus_tag="LNC71_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK6466194.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="LNC71_001109"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="LNC71_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBK6466195.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="LNC71_001110"
     CDS             14643..16016
                     /locus_tag="LNC71_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK6466196.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="LNC71_001111"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="LNC71_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBK6466197.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="LNC71_001112"
     CDS             17071..17511
                     /locus_tag="LNC71_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK6466198.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="LNC71_001113"
     CDS             17655..18356
                     /locus_tag="LNC71_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBK6466199.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="LNC71_001114"
     CDS             18353..19474
                     /locus_tag="LNC71_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBK6466200.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="LNC71_001115"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="LNC71_001115"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBK6466201.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="LNC71_001116"
     CDS             20853..21761
                     /locus_tag="LNC71_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBK6466202.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="LNC71_001117"
     CDS             21796..22068
                     /locus_tag="LNC71_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBK6466203.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="LNC71_001118"
     CDS             22079..23125
                     /locus_tag="LNC71_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBK6466204.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="LNC71_001119"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="LNC71_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBK6466205.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26822)
                     /locus_tag="LNC71_001120"
     CDS             complement(26136..26822)
                     /locus_tag="LNC71_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466206.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26800..27294)
                     /locus_tag="LNC71_001121"
     CDS             complement(26800..27294)
                     /locus_tag="LNC71_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK6466207.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="LNC71_001122"
     CDS             complement(27314..28534)
                     /locus_tag="LNC71_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466208.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="LNC71_001123"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="LNC71_001123"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBK6466209.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30712..31623
                     /locus_tag="LNC71_001124"
     CDS             30712..31623
                     /locus_tag="LNC71_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6466210.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        11002 a         5654 c         6975 g         8921 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggatgagca agatagtgtg aaataactta taatcaatcg aaaaacctat
    30541 taaataaacc tttttgagta gatgcaacac tgaggattat ctgtataaaa gattttaaac
    30601 tgtcatttgg ttatcacggc gatactaaaa ttaacaaaat tggaatattt ttcttgaaga
    30661 tttttagctg tcagtatatg atgaaagcat ctcataggag gtgctttttt catggttgat
    30721 aattcaagtg gaagggttct taaatctcta agaaaagaga aaaaactatc tcaaaaaaag
    30781 ttagctgatt tagcaggtat ttcacaaagt actttagtta aatatgaaaa agggtcaaga
    30841 aaaatcccta aagacgtaga taatactcta agcaagattc tgaatataga aactctaata
    30901 aaagatgagg aagataagat tgagattctc atcggtaaac taattgcata cagagatatg
    30961 aataagttac ttaacaaaga gttagctgat aatataggaa ttagtgaagt attactcagt
    31021 tatgtgttaa atagaaagag aaatccgtca aaagaaatgc aaaaaaaaat cgatattttt
    31081 ctattaagta atgaaaaaga aattttgaag gagattaatc gtgatagtga gatatttagt
    31141 ttatcaaaag acgataaaat tgttatggga aaaagaatac gagaagtccg aaagaaccgg
    31201 gaagagacgc ttgaaaaatt tggtaaaaac tttaccatct atacaggcaa aaatgttata
    31261 agtagatggg aaaaaggtat taatattcca gatattgaaa aactaatgaa tattgcctat
    31321 cttggtaagg tcactgttcc ttaccttatg tacggagaag attataagaa tatacttcca
    31381 aaagatgaaa gagttagcga ttttaaaaag ataaattctt ttagtatggg attaaggatg
    31441 aggaaaatta ggaaagatta ttatctagaa agggaggagt ttggaaagct tttttctcct
    31501 tcaatttcaa aatggtccat agataggtat gagaatggta gagatatacc caatactaat
    31561 cgaattatcc aatatgctta tataggaaat ctttctttag aatttttgat ttacgggata
    31621 taaaaatagt ttacgtcgat acttattaag ttatcgacgt tttttattat agaaaaaatt
    31681 tataaaagga ggtttcttga tatgataaga aacaaaaagg gtttgttgaa agttgcttta
    31741 ctggcagctt ttttctaagt aactttttga caagctcaat ggcaacacaa gcggaagaag
    31801 taatacaaga actaactgtt atgaataaca gctagataaa atgggctatg tatgttatcc
    31861 aacaatttag taatcactag aagcctagcg aaagttcgct tgataagtta tagatgagta
    31921 ttgatttgta gtgtttccaa atcaaaaggg tacagagcca catgaaatat gttgtcctct
    31981 gcagcgtcag gatgggaatc cataccatgg agattcggag atctgtgagg gaatacttac
    32041 gtggttaata tgtcagttgt gttggacgta gtaatggtca tagtgggtag attgactgaa
    32101 ttcttatatg agaaaatgaa accaacaaga agatagggtt atttatacag aattggggat
    32161 cttttctgga aaagtaatcg gataatgcga gattcgtacg gtctttttta aatttctcta
    32221 aaaaaaacgg catcgaatgg gttgggtgat tgataagcaa tgaatcatag actgatatag
    32281 aataaaaaat gattctattt atctttttac atctatcgtt ttttcaagta gccgcaaatt
    32341 atatcagcat tcaacaattt ttgtatacaa gttctagtaa aaggatctga agaaaaagtt
    32401 ggttgttgtg aagataaaaa aagaatcagg ttttactgag tttttcttca aaatttgtgt
    32461 tattaggtta ggacaaagat aagaagaagt atgaagattt aaaaaaggaa tgaggaaaag
    32521 atagtaattt ccgattattg gagttttttt at
//
LOCUS       DAFCHQ010000022        32181 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000022 DAFCHQ010000000
VERSION     DAFCHQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.022"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(122..502)
                     /gene="yajC"
                     /locus_tag="LNC71_001125"
     CDS             complement(122..502)
                     /gene="yajC"
                     /locus_tag="LNC71_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBK6466211.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(599..1744)
                     /gene="tgt"
                     /locus_tag="LNC71_001126"
     CDS             complement(599..1744)
                     /gene="tgt"
                     /locus_tag="LNC71_001126"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBK6466212.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1962..2660
                     /locus_tag="LNC71_001127"
     CDS             1962..2660
                     /locus_tag="LNC71_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBK6466213.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2828..5467)
                     /locus_tag="LNC71_001128"
     CDS             complement(2828..5467)
                     /locus_tag="LNC71_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6466214.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6086..7117)
                     /gene="queA"
                     /locus_tag="LNC71_001129"
     CDS             complement(6086..7117)
                     /gene="queA"
                     /locus_tag="LNC71_001129"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBK6466215.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7310..8484
                     /locus_tag="LNC71_001130"
                     /pseudo
     CDS             7310..8484
                     /locus_tag="LNC71_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            8488..9129
                     /locus_tag="LNC71_001131"
     CDS             8488..9129
                     /locus_tag="LNC71_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466216.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9126..10043
                     /locus_tag="LNC71_001132"
     CDS             9126..10043
                     /locus_tag="LNC71_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6466217.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10040..10711
                     /locus_tag="LNC71_001133"
     CDS             10040..10711
                     /locus_tag="LNC71_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466218.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11252..11740)
                     /locus_tag="LNC71_001134"
     CDS             complement(11252..11740)
                     /locus_tag="LNC71_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBK6466219.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11941..12207)
                     /locus_tag="LNC71_001135"
     CDS             complement(11941..12207)
                     /locus_tag="LNC71_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK6466220.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12207..12470)
                     /locus_tag="LNC71_001136"
     CDS             complement(12207..12470)
                     /locus_tag="LNC71_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6466221.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12946..13131
                     /locus_tag="LNC71_001137"
     CDS             12946..13131
                     /locus_tag="LNC71_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6466222.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13273..15096)
                     /locus_tag="LNC71_001138"
     CDS             complement(13273..15096)
                     /locus_tag="LNC71_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBK6466223.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15694..18399)
                     /locus_tag="LNC71_001139"
     CDS             complement(15694..18399)
                     /locus_tag="LNC71_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBK6466224.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18705..19652)
                     /locus_tag="LNC71_001140"
     CDS             complement(18705..19652)
                     /locus_tag="LNC71_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBK6466225.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19842..20111
                     /gene="rpsN"
                     /locus_tag="LNC71_001141"
     CDS             19842..20111
                     /gene="rpsN"
                     /locus_tag="LNC71_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK6466226.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20287..20649)
                     /locus_tag="LNC71_001142"
     CDS             complement(20287..20649)
                     /locus_tag="LNC71_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6466227.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20660..21781)
                     /locus_tag="LNC71_001143"
     CDS             complement(20660..21781)
                     /locus_tag="LNC71_001143"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBK6466228.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21797..22147)
                     /locus_tag="LNC71_001144"
     CDS             complement(21797..22147)
                     /locus_tag="LNC71_001144"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBK6466229.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22277..23788)
                     /locus_tag="LNC71_001145"
     CDS             complement(22277..23788)
                     /locus_tag="LNC71_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBK6466230.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24110..25462)
                     /locus_tag="LNC71_001146"
     CDS             complement(24110..25462)
                     /locus_tag="LNC71_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBK6466231.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25615..26691)
                     /locus_tag="LNC71_001147"
     CDS             complement(25615..26691)
                     /locus_tag="LNC71_001147"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBK6466232.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26887..28632
                     /locus_tag="LNC71_001148"
     CDS             26887..28632
                     /locus_tag="LNC71_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBK6466233.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28912..29349)
                     /locus_tag="LNC71_001149"
     CDS             complement(28912..29349)
                     /locus_tag="LNC71_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBK6466234.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29333..30706)
                     /locus_tag="LNC71_001150"
     CDS             complement(29333..30706)
                     /locus_tag="LNC71_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466235.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31028..31516)
                     /locus_tag="LNC71_001151"
     CDS             complement(31028..31516)
                     /locus_tag="LNC71_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466236.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31538..32140)
                     /locus_tag="LNC71_001152"
     CDS             complement(31538..32140)
                     /locus_tag="LNC71_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6466237.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9200 a         6882 c         5942 g        10157 t
ORIGIN      
        1 gacacacccg attattcaat attgagcaat atatcccatg ctcttatctc tttctttttt
       61 gaataaaaaa tgaggtagga catctttctg ccctacctcg tctttaaata agctttgata
      121 cttatttttc ttccttctct tctgtctctt ccagcgtttc tgttgtttca gtttttactt
      181 caactgtctc agctggttct tctgttgttt caacagtagt cgttgcatca tttgttacga
      241 cagtccctgg tttgaccgtt ttgattgctg cacgatcgta ctcaaggaag atcccttcgc
      301 agtctaaaac aactgtccgt ttgtcattgt cgatttctga aacaactcca tgtaatccgc
      361 cgatcgttac gactgaatca cctggtttca tgctatctaa caagttttga cgttcttgct
      421 gttgtttctt ttgcgaacgt gacataaaaa accacattgc taatagcgcg ataaacatca
      481 caagcattgg taatgttccc atttttttca cctcactaat tctgtatcta caaaaaacac
      541 tttagcagaa aaatgctgat aacactagtt ttttacttaa tttttaaaag attttccttt
      601 agaagctttt tgcgttttct ttattgaacc cgtattcttc aaaaaaggct tctctaaatt
      661 caagcaaatt atcatccatg attgcttgac gtacttgttt cattagattc aacaagaagt
      721 ataaattgtg gtaagaagtc aaacgaatcc caaatgtttc atcacatttg atcaaatggc
      781 ggatataggc acgtgtatag tttttacatg tataacaatc acatttctca tcaattggac
      841 ggaaatcatg ggcatatttc gcatttttca ctactagacg gccttgagaa gtcatacatg
      901 tgccatttcg agcgattcga gtaggcaaga cacagtcaaa catgtctact ccacggataa
      961 caccatcgat caatgaatca gcagcaccga cacccatcaa gtaacgtggt ttgttttcgg
     1021 ggatcaacgg tgtagtgaat tctaatacac gattcatttc tgatttaggt tccccgacag
     1081 ataacccgcc gattgagtaa ccagggaaat ccatcccgac tagatctttc gcgctttgac
     1141 gacgaagatc ttcaaaccct gctccttgga tgattccaaa taatccttga cggtctggat
     1201 tagcatgagc tttcaatcct cgttctgccc atcgagacgt acgttctact gattttttca
     1261 cgtagtcgta actttcatcg aatggtggac attcgtcaaa actcatcatg atatcagagc
     1321 ccaatttatt ttggatgttg attgcttttt ctggtgataa aaacatacgc gaaccgttca
     1381 agtgattttt gaaatgcacg ccttcttctt cgatattccg catatcactt aatgaaaaaa
     1441 cttgaaagcc gcctgaatct gttaaaatcg gttgatccca gttcataaat ttatgcagtc
     1501 cgccagcttc ttctactaag tcttcaccag ggcgtagcca taaatgatat gtattactta
     1561 aaataacgcc agcacccatt tctttcaact cttctgggga cattgttttt actgtagcta
     1621 atgttcctac tggcatgaac attggtgtgg ggaaagttcc gtgtggcgtg atcaattccc
     1681 ctaaacgcgc gccagtatgt ttttctttct tgattaaacg atagcgaatg gcaggttgcg
     1741 tcattcattt ccctactttc tatataaaat atcttgacaa ctcccaccat cataatcgtt
     1801 tcaagtgctt tttgcaagtt ccctcggctt ccttctactt tatttaatat tctttttttt
     1861 atcctttttt tatgaataac aaaaaaaaga aaaacattat gacagaaata ttttttgata
     1921 catggtatat tggtgttatt aaagaaaagg agggaaaaat catgcaatct tctgcagaat
     1981 taaaacgcca agcaaaagag agtctaaaag gacgttgggg acaagctgtt ttactaaatc
     2041 taatccctac attgattggt attgctacta ttcttcttat tatgattcct attatcttgt
     2101 ttgccaccac catatatgat ggatcagcga gcgtctcaga agcgacaact tcaggaggcg
     2161 gtagttcagg aggcggtggc ggaattattt ccagcatcat tagcgctttg ttcatgagcg
     2221 gtatttcttg gacttatctt gatattttac gtggacaaaa agatcagatc gaaccattca
     2281 aagatgcatt tcgtggattc aaaggtttgt ttatcggcgg agtgatcagc ttagctgtgc
     2341 tgatctcgat ctttacaaca ttatggggtt tgctattagt tattcctgga atcattaaaa
     2401 gctattctta ttctcaaagc tattttattt actacgatat cgtccaagaa acaggacaaa
     2461 cacctaaagt actggatacg atcactgcaa gtcgaaaatt gatggatggt tataaaggac
     2521 aacttttctg gttagatctt tcatttatcg gctggcatat cttagcatta gcaactgctg
     2581 gtattggtta tctatggctc agcccataca tttctgcaac aaaagcagca ttttatgaac
     2641 aattgccaaa agatatttaa aagagagtgt gacttgtgac cgagggggtg gcaggagaac
     2701 accgtttatc gagaggcagt aaaagaaaca ttcagcccat tagcaaaaag gaggtgtgac
     2761 aacattcatg tcacacctcc tttttgtata gttccagtaa aaagaaaatc aacttagaaa
     2821 cagttaatca ttcctcttct tctgcttccg gtaatcgatg gaacttgatc agttcattca
     2881 ccgcgattgg tacgatagat aaaatcccta ccaagaacca gtgattcaaa ctgatgtgta
     2941 ctaggccgaa tagttcttgc gtaaatggca gcaaagcaac agccgttgta atagctaatg
     3001 ccagtaacgc catttctacc aaagctttat tggtcgctaa attgactttg aagatcgatt
     3061 ggctgctgcg tacattgaac acatgcagaa tcgatgaata agctaagatc aagaatgcaa
     3121 cagtttgacc gacatgctga cttgcgtcta cccatgggct gatagtatcg acataactac
     3181 ctaaataata accaaacaag gttacgatgg tgaagacccc tgcatttatg gcaatttttt
     3241 gcaataaacc tcgagcaaag atcccttcat ttttcggaat gggtggctga tccatgatgc
     3301 cgtgttccgc tggctctctt cctaaagcaa atcctggaag tccatcagca actacattga
     3361 caaacagtag ttgcacagct gtaaatggtg ctccccatcc taacagcatc gcaatcaaaa
     3421 caataaagat ctccgaaata ttacagctta gcaaaaagtt gatagcttta cgaatattct
     3481 gataaaccga acgtccttga ctgacagcgt ctacgatcgt cgcaaagttg tcatccgtca
     3541 aaatcatatc tgcggcactt tgcgccacat ctgttcctgt gatccccatg gcacacccaa
     3601 cgttacttgc tttcaacgct ggtgcatcat tcacaccatc cccagtcatc gctacgactg
     3661 cacccgtacg ttgccaagat ttgacgatcc ggatcttgtc ttctggcgtt acccgagcat
     3721 agacagagag agctttgaca cgcgcatcta attcttcgtc tgataattga tgcagttctg
     3781 atcctgaaag tgcttctgac ggatcattca agatacctaa ttctttcgct atggcactgg
     3841 cagtcaccac atgatctcca gtgatcatga ctgttttgat gccagccttt ttcgctcgct
     3901 tgattgctcc tttactttct ggacgtggtg gatcgatcat tccgatcaga ccaatcaaac
     3961 gtaagtcttt ttccagtgct tcagaagaaa tatcttgcgg ttcttcgtca taagtagcat
     4021 aaccaacagc aatcactctc agtgcccgtt taccaaagcg atcattaacg atcgccgctt
     4081 gttctacatc gcctgaacta aagcgcggag caagaacatc gaatgctcct ttggtgattg
     4141 agatgtattt ttcgcccatt tggtgaactg ttgtcatcat ttttcgttca gaatcaaaag
     4201 gaagctcgtt tactcgtgga tatttttctt ctaattctgc ctttgtatga taattctctt
     4261 caacagccct tacgatggct acttctgttg gattaccttt gataacaagg tcgtcatctt
     4321 cttgctcgac cgtcacatct gtacatagtg cagccatttt caatatttcc atcgcgctgt
     4381 tcgtcattga atcttcgata ttcgttactt catcttcttt gctccatacc cggcggacgc
     4441 gcatttcatt ttgtgtcagc gttcctgttt tgtctgagca gataacatta gcggttccta
     4501 gcgtttcaac tgctggcaac tgtcggatga tcgcatgttt cctcgccatc ttctgcacac
     4561 cgtatgcaag tgtcaacgta acaataacag tcaaggtttc cggtacagct gcaacagcca
     4621 aggaaatcga agtcatgaac atctcaagga gtggttctcc ttgcagttca ccaatcacaa
     4681 atactaaagc cgcagccgct aaagcgatca tactgattcg tttgcctagc tgattcaatc
     4741 ggacctgtaa aggtgttttt tgcatagtat tttcgttcaa aagatccgcg atcttaccca
     4801 tttctgtagc catgccaatg gcagtgacga tcgctttccc acgacctgca gcaaccgtac
     4861 atcctttaaa taccatatta agttggtcac ctaatgactc atcttcttca gcaataaatg
     4921 ctgcatcttt ttcaactgct tcactttctc cagtcaacgc cgattcttct acacgtaatt
     4981 gtgatgcttc gatcaaacgg gcatcggcag aaatagcatc accggactct aaaacaagaa
     5041 tatcacccac taccagttgc tctgactcaa cagtggtcac tttgccgtct cgtatgacag
     5101 ttgccgtttg tttattcata tcttgcaagg attccagtgc tttttctgcg tttccttctt
     5161 ggacaatcgc caataccgag ttgatcacaa cgatagcgat gatcagcagt ccttcaaata
     5221 aatccccatg ttctgtagca aatgcggtat aaaaggaaat caccgctgct acaagaagga
     5281 tgatcgttgt aaaatcggac aagcttcgaa agaatttttt gatcatcgat tctttcggcg
     5341 cctcatcgaa tttattcaat ccctgctttt ttcgccgttc atccacttct tgtgaagaca
     5401 acccagtatc tggatctgct tctgttacct tctttacttc ttctattgtc tgcttgtatt
     5461 cttccatcct attcctcctt taaaatcttc gtacaaaaaa aggtacttat tttactataa
     5521 catccttttt ttatttactt caaataaagg gcttatcgat tatttgttaa gaaagcgtaa
     5581 aatttaaagt gcttatattt gcaaacgcta ccaaaggatg ctattatata gatgtaagga
     5641 aggaggatac catcaaatag acagaagaat ttctcgatag aacagtgtaa gcaaacaaag
     5701 ttgttagaag tacaggtttc acccagacat gcgtgtagag tacaaaccta taaatagtga
     5761 atgcgtatgg atgtttggat cacgaagtat ccgaaacaag aagtaatgcg atacgtaaca
     5821 attgaataac gtaaacgtct gaaaattgaa taggaacaag agatgtttca aatgttgaag
     5881 atgtaacgaa aacaaaattt ttgtttaaca tgaagaaatt caacaccgtg ctttgcatgt
     5941 atgatggaaa gttacaatca tttctgttta tttgaatcct tccgaggtac aagaaagccg
     6001 agcttatcga gagctcggct ttttctttgt cataaaatct ttgtaaagct tttttgttat
     6061 tcaaaactag cggcttttca taaccttatt tcacgaacat cgcatctcca aaactaaaga
     6121 aacgatagcg ctcttcaatc gcatgctggt aagcagataa agtcaattct cttcctgcaa
     6181 aggcactaac taacatgact aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt
     6241 ctaccacttt gaattcatag ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt
     6301 tgatttctcc attaaatttc gttccaatcg tttctaatgt tcgaattgaa gttgttccga
     6361 cagccacgat tttcccgcca tttttacgta cttggttcaa tcgctcagcc gcttcttccg
     6421 tcaaacggta gaattcacta tgcatatggt gttcttcaat tttatctacg ctgacaggtc
     6481 ggaatgtacc taaccctaca tgtagtgtca gatagactaa ttcaacaccc tttgcttcga
     6541 tctcatcaag taattctttc gtaaaatgca atccagctgt tggcgctgca gcagaaccat
     6601 tttctttcgc atagacagtt tgatagcgat ctgggtcttc cagacgttct ttgatatatg
     6661 gaggcaatgg catttctccc aatgattcaa gaatctctag aaaaattcct tcatactcaa
     6721 agtcaatgat ccgtccgccg tgttccaatt cttctttcac tactgcttgc aatctgccat
     6781 caccaaaaga gattcttgtc ccaactttgg cacgtttcgc aggtttgatc aatgtctccc
     6841 acgtatctcc ttgtgtattc gttagaagaa ggacttctaa atgcgcacca gtatctggtt
     6901 tttctccata taaacgagca ggcagaactc gtgtattatt cataactagc gcatcaccag
     6961 gctgtaactc ttcaatgatt tcatggaaat gtttatcttc catctctcca gttttccgat
     7021 ccaaaacaag taaacgagaa tgatcgcggt cttttaatgg tgtttgtgcg attaattctt
     7081 ccggcaagtc aaaatcaaaa tcttctgttg ttaacatatc gtcactcact ttctatcatg
     7141 tgcttcatca ttctatcatg tttttcgttc tgttggcaaa atcagcactt tttcacttct
     7201 attttttgtt ttcctaaata aaaaaactac cctatttgta ttttttcatt gttttaggca
     7261 tactagtaat agatagttct tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt
     7321 tcaaaatgtg tcaaaaattt ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc
     7381 atttgaaaag ggtgagttca tttgttttat cggtacgagc ggaagcggaa aaacgacgac
     7441 gatgcggatg atcaaccgaa tgactgatcc aacacaaggg aaaattttga ttgatggaaa
     7501 atccatccaa gaaattgatc cagtggaatt gcgacgtcag atcggctatg ttatccaaag
     7561 tattggattg atgcctcaca tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt
     7621 gaaagtcagt caagaagaac ggaataaaac agctgaaaaa atgattgatc ttgtggaatt
     7681 gccccgtgag atgttagatc gctacccaca tgaattatct ggtggtcagc agcagcgtat
     7741 tggagtcgtt cgtgcccttg cagcgaatca agatatcatc ttgatggacg agccattcgg
     7801 tgcgcttgat ccaatcactc gtgattcctt gcaagacctt gtcaaagatt tacaagagcg
     7861 tttgggaaaa acgatcgtat ttgtaaccca tgacatggat gaagcgatta aactagcaag
     7921 ccgtatcgcc atcatgagtg aaggccgtgt gatcaatttg atacaccaga aaatatttta
     7981 cgtcatcctg caaacgattt cgtagaagag ttgattggag aagatcgcct actacaagcc
     8041 aagccaaatg cgacaagagt cggcgaagtt atgctaaata cagcaatcac gattacaccg
     8101 gaaaaatcat tacaagaagc aatcaaactg atgagggaaa aacgtgtaga tacgttgtta
     8161 gttaccgata attcgaatgt attaaaagga tttatcgatg tagaaacgtt gaataaaaaa
     8221 cgaggaaaag tatccagtgt cggcgacatt ttgaataaac atgtttttta cgtaaaagaa
     8281 tccgcctatc ttcgtgatac cttgcaacgc attttaaaac gcgggctgaa atacgtgccg
     8341 gtcgttgatg aacagaacaa agtcgtaggt atcctgactc gtgcatcatt agtggatatc
     8401 gtctacgatg tgatttgggg agaagatccc gcaactactg atgttgcaga gtcagctact
     8461 cctgaagaat ctaaggaggt ctgacctatg cagcagtttt ttcatcagca cggttcagaa
     8521 attctctcaa aaggttttga acatctttat atttcaggac ttgctttgat cattggtatc
     8581 atcattgctg taccgcttgg tgtcttactg acacgttttc caaaagctgc cacgattgtg
     8641 attggcttaa ccagtgcctt gcagacaatc ccttctcttg ctttattggc attgatgatc
     8701 ccgctatttg gcgtaggtaa attacctgcg attatcgcac tctttattta ttcattattg
     8761 ccgattttac gtaatacgta cattgggatg aaaaatgtcg attggaatta tcgggatgtg
     8821 gcaaaaggca tggggatgac tgagttccaa tccatttttt ccgttgaatt gcccattgct
     8881 atgccaacga tcatggctgg tattcgttta gctgccgttt acgtgattgc atgggctaca
     8941 cttgcctctt atatcggcgc tggtggttta ggcgatttga tttttagtgg actgaataat
     9001 taccaaccag atctcatttt ggcaggtacg atccctgtaa caattttagc actattggct
     9061 gattggcttc taggtctctt ggaacaccgg ctgacaccga tcgcacttag agaggagaat
     9121 gaagaatgaa acgtatacta aaaatgacca tggttctttt ctctcttctt atacttgcga
     9181 gttgttcctt ccctggtctt gccagcaata cagatgaaga caccatttcg atcacaggtg
     9241 gaatcacttc agaagcacag atcttagcaa gtctagttgc cggaatggtt gaacattata
     9301 cagataaaaa taccgcaatc atcaacaatc tggctactac aactatcaac catcaagcga
     9361 tgatgaacgg agatgcttca atttcagcgg ctcgctacac aggaacagat ttaacgacta
     9421 cattgaacct tcctcctgaa aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat
     9481 ttgagaaaag atacggtcaa acgtggttcc cgtcctatag ttttgaaaat acgtatgtct
     9541 ttcttgttcg aaaagacacc gcacaaaaat accatctatc taaagtcagc gatctgaaaa
     9601 atgtagcgga tgaattagta gctggtgtag atacttcttg gatcaatcgt aaaggagacg
     9661 gttatgacgg cttccaagaa acttatggct tctccttcaa ttcgatatta cctatgcaga
     9721 ttggacttgt ttatgacgca gtcgaagcag gaaaaatgga tattgttctt ggctactcta
     9781 cggatggacg gatcgccagt tatgatctag tcatgttgga agatgatcgt caattcttcc
     9841 caccatatga tgctgctcca gttgtagaca acaagatatt agctgatacg cctcatttaa
     9901 aagaagcctt aaataagtta ggtcatacca tcagtacgga aaaaatgcag gaattgaact
     9961 acgaagcaga taataatcta gtggaaccat cagtagtagc acaacgtttc ttacaagaaa
    10021 accactattt tgaagggaag tgaagcaatt ggataaaatg aatgtctttc aacaattttt
    10081 ctattatttc caggaaaatg gctcctatat ctttgcacaa tttatccgtc acttcctgat
    10141 ttctatctat ggtgtcctct ttgcagcagt tgtcgggatt ccggttggga tcatgatttc
    10201 taggagacgt aaattagcaa actgggtgat tcgtttggcg aatatcatcc agacgatccc
    10261 ttctctagcc atgatctcca ttttaatcat tggattaggc ctgggagtca atgtcgtgat
    10321 tgttactgta tttttgtatt ccctcttacc gatcatcaag aatacgtata ccgggatgat
    10381 ccaagtagac aaaaacatcc ttgatgtcgg caaaggcatg gggatgactg ctagacaacg
    10441 actattcatg gtagagttac cactctctgt ttctgttatc atggcaggca taagaaacgc
    10501 gttagttgtt gccatcggga taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg
    10561 agatatcatt atccgaggaa ccaatgcaac agagggcaca tcgattattt tagcaggtgc
    10621 attgccaact gcattaatgg cgatcatcac ggactggctt ttaggtattt tggaaagacg
    10681 gctagatcca gccagccgta catcacgctg atgttattgt aaaaaacaaa agcttctgcg
    10741 accatttgtt ggtcgcaaag gcttttttag ttctgtcatt tttaaggcaa taatccactc
    10801 tgattcttcc ctccttatgg gcaaagagtt gcttttctta gctatttatc cattccgtat
    10861 actccaactt catactgcca acgggttcat atctgcatcc accgactaag tttttttctt
    10921 atccgctaag gcattttgcc aatccatttt cccatcactc gtcatggaat tcataccaca
    10981 tgatctctaa atactcgtct gcttgttctt tgaattgggc atcaattctt acatcctttg
    11041 gcgtggcttt catttaagcc ttttccatca gtcctcgatt gttcgatgcc ttcagctgca
    11101 tcatcaaaac tggtgactgc cgtatcggta acttcgatca tcttttatac aatcgggaac
    11161 ttgccatcct catccttggc taaaaacata tctagccatt ccagcttctc gccatgggag
    11221 gtttgcggtt cctaaaaccg gtacacgatt cttatggact gcatccgtcg caactagact
    11281 tggcgatacg atgatccttc accagaagag cacccccgta aaccaattga tcgatatatt
    11341 gccaataaga aaagacattg gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga
    11401 tactgctatt catggccgaa agcgccacga ctttaacttc ttccgtttga gtgacattca
    11461 tgttcggcaa tgtcgctttg gatacccgct ctgctaaagg aacgacactg gtattgtatt
    11521 tcgtatctgt atcttttcca aaagaccatg ccaacgaatc ctctgggaac caataagacg
    11581 actccggttg attgtccatt gcttttttca acatactttt ggctgtttct cgataaacga
    11641 catttccaat tgtcttcgcc tcactttggc gataatcgat ggtcgttgcc actgccaaca
    11701 ctaacaacga tgctccaatt ttactgattt tccttgtcac acttcttcct ccttgtttta
    11761 cataaacccg tcaacattcc attcctaaac tcaaacaatg acccttcaaa ggtggagata
    11821 taggaacaaa ttaaagaaat catgcaataa atccaagaag gttggtttta gaacaaaaaa
    11881 agagtctcct ttttggggag actctttgtt aactatctaa aaaggttggc tcttctcact
    11941 tcactcataa tgtgtccagg cagaataaat ttctacaact ttattttcct catcaacttt
    12001 gtaaaccact cgatgctgtc tattcagcct gcgtgagtat ctgccttcat gtgtaggtct
    12061 cagcttttca aaagactgcg tgggcatata tggatcttcc ttcaatgtct gaatcacttc
    12121 ttcaaattgt ttctttaaat tcgtttgctt gatttttctt aaatccactt tcgcagagtt
    12181 tttgattgcg actgtatagt tactcatcaa agagcttccc aatcaatatc atcgatattc
    12241 gtaaagctac tgtcgtcttt ttcgcggtct cgtactttat ctaaagtacc tgtttgctca
    12301 agaaaaaggg tttcttgaat cgctctccag tcttccagcc caatcaatac agcattgttt
    12361 ccactacgat cactaataat ctcgatttca gtatgatttt catttacctc tttcaataac
    12421 tgataaaaat ttttacgagc agtgctagga ttcatcgttg tattagccat tgtctctcct
    12481 cctttttctg atataattat agcgtacttg attaagtacg tcaaaaaaat acaataaagc
    12541 acttaaattc agcaaacttt tcctcttttt tacatagaga aaaagacccc tttttaggga
    12601 ggatctttgt ccacgctcac ttcttactcc tttacgacta gcggtagcca aacgtaaagt
    12661 aattggtaac aacgtatcat ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca
    12721 acaacaccaa tacacgaagg ttcctcgttg cagtgtgact tgattgatat cttgaataac
    12781 ctgaggatac gcacgaaaga tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc
    12841 ttctactgcc tcaaaaatga ttgcctcttt cgtctcctgt cattcatgtt cactatcgat
    12901 ttgatacttg cttagaccgt tgggtttgtg aatcaccaat taaaaatggc actgagttat
    12961 ggtaacaaaa aatggtcact tgacgaggct gagattccta cttatgaaga cacatggcac
    13021 ttaagagtcg aggtaaagga agaagctgaa aatataggag gctgtacatt atttggcaaa
    13081 ggattcggaa attatgatca agacatcaaa atcaaaatgt tttacagcta aataggaaga
    13141 ataataacat ttcatacgct gttcccatct ttaatggctt atagagggca cctttagctg
    13201 agaataagac aaaacagacc ctctagcttc aattaagagc tagagggtct gtttttgatt
    13261 ttaaaacttg gtttatttct tcaaatggta gctataggtg ctgacaacga tattttcacg
    13321 ccaagacaat cgcaatctta accgcaaact tgtttggtta tggagaatat tctgccagcg
    13381 tctgtttggt acaaattgag gaatcaacac agtagttgta tagttttgtt tcgctgcatt
    13441 tttacttacg atatctacat aacggataat tggattttca atcgagcgat aagaagagtg
    13501 gacgattgaa aaacggacat ctgggaaatg ctttttaaat tctgcttcga tttctttttc
    13561 tttttcgaca tcttcgtcta gcgaaacatg catcgcaacg acataatcac cgatcgaacg
    13621 tgcgtaattc aacgcaccga tatttacccg tgtcacattc ccgactaata caagaacggt
    13681 attcccgtca taatcatgta gttttgcatc attttctaaa cgaagctgtt ctgctacttt
    13741 tttataatga tcatggatct tataaaaaat gaacataacg attggcataa tgatgaagaa
    13801 aggccaaatg tcccctaacc gatagacaaa caaaatcgca atgattgcat atgagatgaa
    13861 cgcacccgta atattggcaa tcgatttgct taaccatctt tttccttctt ttttccattt
    13921 gacgaccatc cctgtttgag ataaagcaaa tggaatgaac acgccgatag agtataacgg
    13981 aatcaagcgt tctgttgagc catggaaaat aaacagcaat acgatcgaac ctagcgctag
    14041 tgtgataatc ccattggaat agcctagtcg atccccacga tcttgataca tgtgaggcat
    14101 gaatttatct tttgccagat tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc
    14161 agcaactgct aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc
    14221 aaagacagct ttaccaattt gagacaaaac agtgacttct ttttcaggaa cgatcccata
    14281 ccaatagttg ataaatgtga tccccacgaa gaagaaacct aagatcaatg ccatcatcgt
    14341 caatgtagca gcggcatttt ttgctcttgg cttcttaaag aatggtacag cattactaat
    14401 cgcctccacc ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat
    14461 agaaacaccc ggcacagccg ctcctggaag tgcagtagct tgcaatggca cagcacctgt
    14521 cacgatatta tataaaccaa caacgatcag taacgagatc acaaggataa atgtgtacac
    14581 cggaaataat aggaacgatg cagattccct cagcccacga agattcagca tcatcagtag
    14641 caaaacgatt gtcacagaaa tagcgacttg atgaccgtag agtgctggca ccgctgatgt
    14701 gatcgcttca gcacctgctg agacagatac tgctactgtc aacatgtagt cgatcaataa
    14761 agagccaccg gaaatcaagc cagcattctt tccaagattc tcactgctga cgacatatgc
    14821 tcccccgcca tgaggataag catggatgat ttgacgatat gatagcgtta atgaaatcaa
    14881 taaaataata acaaaagcgg caatgggtag cgaataccag atggctgcag cagacaaagc
    14941 gactaaaacg acaacgattt gttccgtacc atatgcaata gatgataacg catcagacga
    15001 caataatgcc aaagcagcaa atcgggttaa tttgtgctca tcattttcag cagattttaa
    15061 aggcttacct actaacaacc tttttaagta atccaatttc taaactcctt tacacacaat
    15121 tttacttcaa gttttatagt cgaaaattta tagtaaacaa gatgttatag tacgctcact
    15181 gaccttaaaa gtcaataaac tttaagaaaa taattggaaa aaaacagaaa aatatcaagc
    15241 gctttttcaa acgctttaat acagcaaaaa tctaacattt ttttatgggc tttttctttg
    15301 aattctataa taaaaggata gaacttgact tagaaaatca agttctatcc tttttgatat
    15361 cttaactttt ggaagagaga tgtaaggctg ctttctaccc atcattctta ctactcgaag
    15421 catagcgcag ttgtcacaac ctcttgataa acggtgttta aaagcttgca tctgcggtaa
    15481 taagcccaac caaattaaaa acataagaag ctgtttttat ttggtcgttc ttattacttg
    15541 atgcagagca gcttttttac aacctcttgg tacacggtgt tcaatcagct gaccctcggt
    15601 attagctcaa aaaaacggca aaatatgagg aactattttt ccattttttt ctctaatact
    15661 caggtcaaaa cactgatttc acaacctctt tttttaatga aaaatatggc tctcatgcgc
    15721 cgcttctttg aatttcgtca ttttttcttc gatgaacgga tagattgccg ttcctacgat
    15781 tccaaagaca acaaacaaac cgatcatgat ccagattgct ttcgtagcat ttggccaata
    15841 aattcctcct gcagattccc gtaacaagtt taccgcatga gtaaatggca agaacggatt
    15901 gatcatttgg aagaatttcc ctgagacttg gatcggatag ttcccgcctc cgccagagat
    15961 cgacagtact aagatgataa tagcgatccc tttaccgaca ttatcgaata atgcagccaa
    16021 gacatagacg atcatcatga acgctaaggc gatcaagaca gcaaatgaca cgctgtagac
    16081 cggattttgg acatctactc ctagtaagaa aatattccct aatgtaacga tcaagctttg
    16141 cgcaatcgcc attgtcaaaa atgtcagcat acgagcaacg aattgttctc gtttcgtaaa
    16201 attgtttcgg tctttctttc ctaaatagta ttctgtcgta gcaacacttg aaagaagaac
    16261 agcgccgacc cacagacata gtgcagtata aaacggggtg ctagctgaac cgttatttgc
    16321 gattggataa agcgcattgg tttgtagctc aacaggttgt gtaaagaagt cactttcttt
    16381 tgttgcgtct agtttcaata atttgatgac ttcgcctaga tcgacttctt gttcgccttt
    16441 tcggatcgcg tctgctgctt tttggatacc agctttgata tttggccagt cattgttgat
    16501 caagtccgca gcagcgttca cagctgtttc gacttctggc attttttcat ttactgttgt
    16561 caatgtaccg gtgatttcat tttttagtgc tggccaatca ttttggataa aatcagcggc
    16621 caatcctaat ttttgctgaa tgatcggtaa ttcattgttg tataggtctg ccccttcatt
    16681 gatgccattg acgatggtat ccatatttcc attcagcatt gtattggcat catggatttc
    16741 ttgtccaatt gcaggcattt ctttttgata tttttctaaa attgccactg cattcgttac
    16801 agttgctttc gttgagctga gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc
    16861 gccttgtgca gtcccaatcg tatcgatgac ttggtctagt agagaagtga cagtggaact
    16921 gatttgacct gtattgatac cagcaatgat gccattgata ttattaacaa cggcatcaat
    16981 ttgatctagc aattcatcaa tagccttcgt gtcttcattt tgaacagctg tgttcaaagc
    17041 agataggcgg ttttgcagat tgcttagcaa gggttttaat tgatttagct gatcaataat
    17101 tggctgtaat ttcgtatttc cagcacttgt ttgcaattta gtcaggaact cgaccagttt
    17161 atcgattgca ttttgttgtt gagatattcc gtcaataaag ccgttcaata cattgctgat
    17221 gtttttcttt tcatcatcag tcagcttacc atcttcaata gctgttctta cagtatctat
    17281 taacgaacct gtcgttgttg ccacttgtcc aattgacttc aatgtaacat ctacactgtt
    17341 tgtgatgctt ggtatcgcat ctttcagctg agaggcacct gattttgttt ggtttgctag
    17401 atcacttgcc tgattttcta aatttttcac atctggcaag atatcttgta cttgttggat
    17461 gatcgtcaag ccttgttttg cttcatcgat tccttggttc attgtctctt ggatcgaagc
    17521 aaaatcgccg tcgatctctg ctagttgctt accggcattt tggatctctg ggattttttc
    17581 ttgcaaatca aggatgattt ttgcttgatc tttcaactca ggcattttgt cattcaatgc
    17641 aacgactttt tcacccatct catctacttt agggatgtaa tccacgaatt catttgcttt
    17701 attcagtttt tccttgattt caggtagttg actttgtaat tcgaggactt gttttgtgta
    17761 gccgtcgatc gtgtccaaat tttcatctgt tgataaaatc atatctttaa ctttattgat
    17821 acttactagg ttcgtatcga tgtcgtaacc tatctcattg aatactttca ataaagtcga
    17881 actggctgtt ttgataaatt catttgtgat ttgcgattgg atcgaacttg ctcctttatc
    17941 ggtgattttc ggtgcaatcg cattgatttt ttcatttacc gtatattcga tttttggttt
    18001 cttgatatca ccactagtaa aactcagcag atcttctgag aaatcctttg gcaagtaaat
    18061 acctgcgtaa tactttccgg aacggactcc gtcttctaat tgttcttttg aatcaacgaa
    18121 ttgccatcct aattgtttat ttttatgaag cgattcgatg acttgctccc caatggccac
    18181 ttctttacct tgaaactctg ctggcttgtc tgcactgtaa acagcaattg gcagctctcc
    18241 agtatttcca tatggatccc ataatgcttt gatgttaaac cacgcatata gcgaagggat
    18301 gatcatgatc gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc
    18361 aagcaaatat aatcgaagcg tatttttgat tgactgcata gttctccccc ttcttctttt
    18421 ttcagctgaa ttactttacc atgtatttca tacttttttc aatcggcttt tttcaaaaaa
    18481 gtcgaatttt tggacatatc acggcaattg tctcattgta atctcttaca tattgagaaa
    18541 taattaagga ttacagcctt tactttatca tgaaataaaa gggaaagata attttatgcc
    18601 aaagttttta tctattatac gtatacgcaa aaaagctaaa acattttttg tctttgcccc
    18661 tacaaaaatg tttcagcttt tttcgttttt tattcctgta tttcttattt ttcgaatgac
    18721 aggatgattt tcccagcagg aggatgtcct tcaactagct gatgtgcttg gatgacatgc
    18781 ttaagtgaag cagacagatt cattgaaata aaaacatgaa aggctccttt ttggtaggct
    18841 ttgattatgc cagcaaaggc ttctgcatct agatattcct ttcttggaac aaacgattca
    18901 taaaagcctt cctttttttg ccgcaaatct agatctggga ggtcatttaa tgcaacatac
    18961 ctgccgcctg gtttcatgat ctggatgccg gtttctcctt tgatacttcc tttcgtcgca
    19021 tcgattacaa ggtctgcctg atcagcaaat tgcagacctg gatttgtttt atcataagca
    19081 gcaaatgctg aagctcctaa tttttttacc ttttcctcat ttttactgga tgcactggtt
    19141 aagattcgga ttcccttttc atggagaagc tggatcagac ttgacccgac cgcacctgat
    19201 gctcctagta tcattacagt atctgtcggc tggatttccg tcaaatggtt gattagatta
    19261 taagccgtaa ttcctggcgt gaccattccg gctgcttctt cccaactcat tttatctgga
    19321 atttttgcta cttttgctga tggcaacacg acttcttctc cgtaagtccc gccgactgca
    19381 tggacagcta ctcgatcacc tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca
    19441 ataccagcgc catcattacc tggtacataa gggaatttca atgttcgtat ctctttcatt
    19501 tctcctaaac gcaaggcaac atcataagga ttcacactga atgcttttat ttctacacga
    19561 acatgtccag aaccgacttc tctgggatga gcatcgattt cttcaaaaac atcttgtgct
    19621 tctccgtaac cgttaattgc aaatcttcgc atggtcacca tctccctttt tctttaagtt
    19681 tacgtttatg cctttatttt gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa
    19741 acaaaatagt tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat
    19801 tacgatttac gctaataaaa aataattgga ggaatttgtt gatggctaaa aaatcaaaga
    19861 ttgccaaagc aaaaaaacag caagaaacga ttgaacgcta tgctgcttta cgccaggaat
    19921 taaaagcgca aaaagattac gaaggcttgc gtaaacttcc tttagatgct cgtccagaac
    19981 gattgaaaaa tcgtgattta atcgatggac gccctcgcgg atacatgcgt aaattcggga
    20041 tgtcccgtat aaaattccgt gaattagcac ataaaggttt gattccagga gtaaaaaaag
    20101 ccagctggta atccaagtct ggctgtaatg taaaggagga gggcgcatgg accactcctt
    20161 ggatcatcaa aaaagatacg ttcgcttctt ctagcagaaa tgctggtact agaagcagac
    20221 gtatcttttt ttacgcacag ctggtccttt tttatatgta aaggctgcat ggttacttcg
    20281 ctatatttat ttagtaacag gagtcagtgc ttcgataccc acactaattt tcaatgctcg
    20341 gtcaacttct gccataatgg gtcgatccaa atggcagacc ttttctttca atcttgattt
    20401 atcgatcgtt ctgatctgct ccaacaggat aacggagtct ctttctattc ctgttccagt
    20461 agctttgatc ccaatgtgag tcggaagctt aggtttagcc atttttgccg ttatggccgc
    20521 aacaatgatc gtcgggctaa aatggttacc tagattattt tgaataacca aaactgggcg
    20581 tgttccgcct tgctccgatc ccacgactgg agaaagatca gcaaaataaa tatctcctct
    20641 ttttaccata ggatcctcct tattcttgat attctcttgg tatccgctgt cctaatccgc
    20701 aagccacttc atagtggatc gtacctaatt gatctgctat atcctgcata gtgatttctt
    20761 tgtctgcatt tttgccgata agcgtcactt ttgttccgac aggaaattct tgaggcaatc
    20821 ggatcatcag ttgatccata catacacgcc caatcgtttc acagtagttc ccttctacca
    20881 atagagaaaa accctgcatc tttcgtggcc agccatctgc ataaccgatc ggtatcgttc
    20941 cgatccactc tgcttcagga gtgatatatg tttctccata gccgattcct tcacctgctg
    21001 ggagtttttt gacttgaatc aattcagaaa ccagctcaag tgctggctgc aatggataaa
    21061 cttccggtag tgcgtgccca gagggattaa gtccgtacat tgctactcca taacgaatca
    21121 tattgccgat tgtttcatca tgccataaag ccgttgcact attgctgaca tgtacatatc
    21181 gaggaagttc gggaagtttt ttcagcactt ctataaagcg ttctttttgc agattccaat
    21241 atgtatcatc tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc cattctaata
    21301 cacgactttc tttgatgaat gcagctgcct cttttacttc ttcaggtgta caaaatccga
    21361 tacgacccat cccagtgtct gcttttagat gaatcgaaag aggcgtctct agcttacttt
    21421 tctctaaaac agcagctgct gcttgcaacc attcccgtgt acctgcagtt actgataaat
    21481 catgagcaac agctacagtc agtgcgtcgt aaggaaccat attcaagata aggatgggct
    21541 gagtaaatcc agcttctctc aactcgactc cttcatctaa atttgaaaca caaaagcccg
    21601 aagctccacc tgcaactgct gcctcagctg tttcaatagc tccatgaccg tatccgttcg
    21661 cttttactac tgcaaacaat tcttttcctt gcggaagtct tgcaacttca tttgctacat
    21721 tttcagttat agctttttta tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca
    21781 tcgtttttcc ttctttctag tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat
    21841 gagtaatact gacccataca ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat
    21901 tttcattttt taaaatctcg acatcttgaa aggtaacttt tccgatccca gttccccaag
    21961 ctttagagaa agcctctttg caagcgtaac gcccgcctaa aaattctact tggcgatgaa
    22021 aaggcaagga ctggaatagt ttcatttcat cagaggtcaa gattcgtgca atgaattttg
    22081 gtttttcttc gatcagccga gtgatcctag gaagttcgac tgcatcgatc ccaattcctt
    22141 ttatcatatg cttttctctt ttcaataaat ttagaacact ctcattctac caaattataa
    22201 ccattttgcc acaaataagt ccacaaataa caaaaatgaa agtttctctt aaaaaagaga
    22261 aactttcatt ttatgtttaa tcgttatttg aacggattac aaatccgcgt tttttatcat
    22321 tgtgacgttt accgccatct ttttggctgt tattattttt gctataaccg ccttttgatt
    22381 tgtttccacg ataattaccg ccgtcacgac gattacggtt gtttccgcca ccacgtttgc
    22441 catttttatt gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg atcttcactg
    22501 gcactgcatc agctggatct tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg
    22561 cagaatattc ttctaataat ttatctgctg tttgcaggta tttatctaga ccattttctg
    22621 caagttttgt ttcgatttgt tcaactgcag cacctaattg acctttgaat gcttcttttt
    22681 cggttggtgg acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt
    22741 agtccatttc atttggtgta acgaaagtca cagacatacc gccttttccg gcacggcctg
    22801 tacgtccgat acggtgaacg tagctttctg gatcttgagg gatatcatag ttgtacacat
    22861 gagtgacacc tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca aggatatcta
    22921 aatgaccgct cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac aaatcgccat
    22981 ggattccttc tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc
    23041 gttttgtacg tccgaagacg attgttaatt caggtgtttg aacatcaaac aagcgagtca
    23101 tgatatcgaa tttttcgtat tcttttgcgc ggacatagta ttgatcgatc aagtcagctg
    23161 tcatttcttt cgctttgatc ttcacatgat gtggattttt catgaattta acgccaatat
    23221 tcttgatagc tggcggcatc gttgctgaga aaagtaatgt ttgtctttga tcaggaactt
    23281 gtgaaatgat tttttcgata tcttccaaga agcccatatt caacatttca tctgcttcat
    23341 ccaaaactaa tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca
    23401 tacgtcctgg tgtacccaca acgatatgtg gacgatcttt caagccacgg atctgacggc
    23461 caatatctgc tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc cctaaacgat
    23521 ataattcttc ttgtgtttgg atagctaatt cacgtgttgg tgcgataact aaaccttgta
    23581 attcgtgacg atctggatcg attttttcta acattggtaa tccaaatgca gctgttttcc
    23641 ctgtacctgt ctgtgcttgt ccaataacgt cttttccagc taatgctagt ggaatcgttt
    23701 ctgcttggat aggcgttgct tcttcaaaac cagcgcgttc gactgatttc aacaattctg
    23761 gtgataattc tagttcttta aatttcaaat gaatcctcct atatggttgt taactgagga
    23821 tgtagctttt ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca
    23881 gtggccacgc acgtttttat ccattcttag cccctgtggc accaccgaaa aatcactttg
    23941 cgaagtttag gcattagaaa agccaaacgg gtcgcgttca gctttatcat tattatgaca
    24001 tctggcttca acaagttgtt tctatggcaa aaccagtcca atttctcttc atctttcggc
    24061 aataggttat tctaacatag tttttcgttt tctgcaagtt tacagatttt tatttcattt
    24121 gctgaagttg ttctaccact tcaaataagc ccatgctgtt gctgcctttt agcataatac
    24181 tatctgttgc ttcaagattt tgtttgatag catcaatcaa tttattcttt tctttttcaa
    24241 aatagaacac aggtagttgg gggtattttt ggttcaattc ttcttttagt gcatgcattt
    24301 gttccccata aaggaaaatc atggcgaatt tctcaggatc gatatgttca ctcatttgcg
    24361 catgcatatt taaagactct ttccctaatt ccagcatatc tgctaacaca gcgattctgc
    24421 gtccttttgt tggcagattt ttaaaagtat ctaataccag tcccatggct gttggatttg
    24481 cattgtagac atcgctcaaa atagctgcac catttcctgc cgttagccat tcggttctgt
    24541 tttgagtgat agaaacatgg gcgagccctt ggcgtatctc aggtatggac agtccaaaga
    24601 agcgcccgat cgtatacgca atcaaagcat tcgttacatt ataacttcca ggcagcggga
    24661 tcgtaaatgt ttctccttct actacaaaat cggtttgtgc ttttcctttt gctgtcactt
    24721 ccgcatgaac atcgccattt ccaataccga atgttgtgac tgtttgtact aatgttgccg
    24781 ttaaaggtgt cagcaacggt tcatctgcgg gcacaacgag taatccgtct gatacaagtc
    24841 cgtcagtgat ttccattttg gctttagcga ttccttctcg cgacccaaga ttttctatgt
    24901 gtgcttcacc gatcattgtg attgctgcag cttttggttg agccataagt gaaagttccg
    24961 tgatttcatt ggcgtgatcc atgcccattt ccaaaacgat cttttctgta tcttggggca
    25021 tgtgcaaaat cgtgtaagga agaccaatat tattgttgta atttccttgc gtcttatatg
    25081 ttttgtattt ttcagacaag acagacgcgg ttagatcttt cgtggtggtt ttaccattgc
    25141 ttcctgtaat tgcaacgact tctgcgccta ctttttccaa gtaataggtt gccaattgct
    25201 gcatggcttt caaaggatct gctacaggaa taactggaaa attttttgga gcttgttcaa
    25261 caggcaagct ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg ataaattcat
    25321 gtccatctcg cgtaccagca agaggaacga ataaacttcc ttcggttatc ttccggctgt
    25381 caaattcgac attggtaatc tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa
    25441 tttcttgtat tgttagattc attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt
    25501 ggcctaaacg gcgggttcgc atgactctca cttccaagta aatttctcta ttcgtattta
    25561 gaagctagaa tgaacgcggt tacgcaatca ctccagcctc ttctttattt ctctttattc
    25621 atttttttca aaaaaagatt gacgctgatg gtatcgattc attcctaact ggatcagttc
    25681 ttcaatcaaa tcaccgtatt tcaagcccat attttcccat aagagtgggt acatactgaa
    25741 ctccgtaaat cctggcatag agtttaattc attcaggaat aattcatttt tatttgtcaa
    25801 aaagaaatcg caccggctca atccgcttcc acctaacatc gtgtaagcta acttcgcgta
    25861 ctcttgcgct ttttgataaa cttcttctgg cacttcggct ggaatctgca tttcgatttt
    25921 attattgata tattttgctt cataatcata gaatgctacg tcttttacga cttcaccagg
    25981 caaagtcgtc cgaacgtctt catttcctaa tacagcaact tcgatttcgc gcgcttcaat
    26041 tccttgttca acgattgctc gagaatcata ctgataggct gttgctaaag cattttgcag
    26101 ctcttctcgg ttttctgctt ttgtaatgcc gacactagaa cccatattcg ccggtttgac
    26161 aaacatcgga taaagcaaag aaccttcaca ttgatcaaat acttttttag gattttcttt
    26221 ccattgattc ttaagtactg gtacataagg aacttgcggc acaccagcag cttgtaaaat
    26281 atacttggtc atgattttat ccatggcaca tgcactggtc aatacgcctg cgccgacata
    26341 aggcatattc aatgtctcta agaagccttg gatcgttcca tcttccccgt ttggcccatg
    26401 taaaactgga aaaacgatgg ctccttcttc tttgatttcg cccggattga tcacttttcc
    26461 tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa agatgcaaga catctttgct
    26521 ggcaggtttt tctgttagta atggaccttt gacccattgt ccttcttttg taataaatac
    26581 gagttgaact tggtaataat tataataaat ggcatttaaa actgaaaatg cggaaagaat
    26641 ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc
    26701 ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa acaaaaataa
    26761 acgaataact tatttataaa aaaacgagcc agcttctaaa agaacgagcg tcatttattt
    26821 tttgtcagct agattatttg tcccgttttt tataaaaagt tatgataaag aaaaggaggg
    26881 atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg agtgttcatc
    26941 aattacaatg tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc
    27001 tttgaagaac taggccccac cttcatcaag ataggacaga tgctttctgt tcgagaagat
    27061 ttgctttctt ctgccttcac tcaaacattc aaaacattac aagatagtgt acctagcgac
    27121 acattttcaa cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt atcagatata
    27181 ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc
    27241 aagctgaaga acggtgacgc ggtagtcgtt aagatccaac accctaacat cgccgaagag
    27301 atccgacttg atcttcaact atttgaacga gcgatccctt tgatcaaata cattcctgaa
    27361 actagcgtag ttgatctaaa aggggtactc caagaagtaa aacgttcctt gatcaatgaa
    27421 atggacttct taaaagaaag tcaaaatggc gaacaattct atcaaaagaa caatggctgg
    27481 aaagagatcc gcagccccaa gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg
    27541 gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact
    27601 tttatcactg gaattcaaaa caagcagctg aaacaagaag ttgcaaagct tttagttgag
    27661 aactttatga aacaggtgtt tgatgatggt tttttccatg ctgatcctca tccagggaat
    27721 ctgctattcc atgtactaac aaaagaagaa cagacacaag ctagccgaaa aactgaaaca
    27781 gtccatgaaa aagagttcgg ttcctttgct tttcgagcct ccacttctgc agaagaccct
    27841 gttgcgccat ataccatcaa ctacatcgat tttggtatga tggggcatct ttctgcagga
    27901 cttaggcaaa aactcacaca agccgtttta gcgctgtata cgaaagatgc ttatcgaatc
    27961 gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac
    28021 caagaactga caagttttct tgaacagtat tatgattctc ctattgatga aatcaacttg
    28081 caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt
    28141 gatatcacgc ttttgctgaa agcatttggg acattagaag gggtgatccg cgtcctcgat
    28201 cctgaagttt ccctcatgga agtcgcttcg ccttttgctc aacattattt tctcactcat
    28261 ctggatgtag aagatacatt gaaacaaagc ggattagact tattggaagg gatgaaggct
    28321 gcgcctaaga tccctcaaca gctccaccat ctattagaaa tgtggacttc gggacaagga
    28381 aaagtcaatt tggaattgaa aaagcaagac aaattattga gcaggatcga aagtatgatc
    28441 aatcggcttg ttttcggtat gattttagct gcattgatcg tagggtcctc cctgcttgtc
    28501 caagcagcac ctgtagaaaa cgcagaggtc gtttccttat tagggatatt tacgtatgct
    28561 attgccgcat tcgtcattat ttttttggca atcgatgcat tgatccaaat gtataagaag
    28621 aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct tcaagaaata agaaggggtt
    28681 ttacataaat tgtttctcaa atttttgtga aaattcggct tatttttcga agaggctact
    28741 tctgaaacac cgagaataaa aaatgtgaaa aagctgttct gcacccagta ataagaacaa
    28801 tcaaaaaggg aacatggaac acatttatcc tgtttccctg ttcccttttt gatgtattcg
    28861 agcataaata aaataccttc gttacctact atcttctgta cttggtaaac cttagtcaat
    28921 tgcctttttt aacgcttgac gaaaagacat atgatctttc tccgtaaagg gttttggacc
    28981 ctttgtcctt atgccagctt ttcttaactt tccactatga taacgttgtt ctagaagcgt
    29041 gccgatattc gcttcgtcaa attcttttcc agagtgatgg aacactcgca cacccttcgg
    29101 caatacaagt gaagctaaac cataatcttg cgtgatcaaa aagtcattct tcttaaccat
    29161 accgacgatt ttataatccg cactatcagc tccgcggtct acgtagacga tttggacatg
    29221 attagcgtat tctttggtcg tataatggtc cacactggtc acgataacca cgtcaagacc
    29281 ataggattct cctaatttta tgacatcttc ttttactgga gaaccatctc catcaatgaa
    29341 tagcctcatt ctttttcgcc ttcttccata aatgataaac agttactaat ccgctcagta
    29401 aaagaccaac attgatccat tttgccaaga taagcagttg tacactttct atcggcactg
    29461 attcatggtt cagcgtgatc aataatagat ggattggctc tcccatttct tttgctaaaa
    29521 caaacaaaag cgtcatcaat aaaaactggt ggatcacact ccctttattt tgagtagtat
    29581 acttgacaac gatccggcga tccattggaa tagctgtatc agtttgataa tattccttat
    29641 ttagccagct gcctgttact acgtgccaga aactaagcag aaagacacct atcgcaaaac
    29701 catttggaat caagaggacc aataaagaag aactcagtcc aagcaattgc atagccaatg
    29761 gagaccatgt taaacgtttc aaaatccatg gtccgactat ttttgctgca atcaagcctg
    29821 ctgcataagg caaatacata aaaatcgaaa gtttttctaa tccgtacagt atgccaacat
    29881 aaaaactgct aaataaaaaa aggaaattcc ctagcattcc atttagaaaa gtaaacagat
    29941 ttagccagct tggcaacttc cattccttct tctggtgtcc taaaacaaac acgaacaaga
    30001 tgaacagcag caagaaacca taaatagcaa gatcaaaaag cctttcatcg aataacaatc
    30061 gggcatttct caaaagaaac aataagccaa agaatatcaa aaataaaaca agctctcgcg
    30121 tagctaccag atttcttttg atgtctttga aatccagttc gtaacgcgga taatgactaa
    30181 ccgtatggta ggccatgacg taaaaaagtg tataaatagt aaagcttgct gcaatctgag
    30241 tcggtgcagg caactcaatc gcttgataca ggagaaatag ccagatagct gcaaataagt
    30301 actttttccc atccatattg ataaaacctt gcgtcttttc gtgaaaattg accgtggtat
    30361 tagcaggtac aagccatgct gcactcagcc ctagcaaaac acttgaaaaa taaaataaag
    30421 gaaaataaaa tacgcctaat attccaagca atgaaccagc accaccaatc agcagagcga
    30481 tcatcagcaa cgtgtaacta tctagaccaa atcgtagact gttgactaaa aatagtccag
    30541 tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg
    30601 ttagtcccaa aaataaaaga taaggaataa aaataaccgt gttacttaag ataaagggcg
    30661 ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca atggctcctt
    30721 aaacagtctt ataactaatc tgctcttcga ttttagcatt aaaggggaaa taaaccaatt
    30781 ttacagaata tattcttgta aaaaaaaata cagaaagaag cataagcttc tttctgcatt
    30841 tttacggtac ttgattactc aagcagacaa gcccttctcc acaaaagtct tgcccgacac
    30901 ccctgcatcc ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc aaaatcaatt
    30961 aggagctctg taaaccagag tccttctcga cccgtcgcat aaatttactt taacacagtt
    31021 cctatgttta tgcgagagct tttttcattt tataatcgac catttgaaat ccttgaccga
    31081 tatctgaaat aagttcttct gtaatctcaa atccttcatg cttataaacc tcgattgctt
    31141 gatgattccc ctgattcact cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt
    31201 cttcatacca gcgaaaaact gaagaagtca atccttgacc acgtacttta tttagcaaat
    31261 ataatttact taaataaatt tttccattta tctcttcata agcagtgtaa ccgacagtct
    31321 tttcttcgct ttttaataaa aagtaggaca caccttctgt cagttctttt tgaatctgtt
    31381 tttttgactg ataagttttc agcatgtaat ccacttgttt ttgaccaatg atcggcacaa
    31441 aaacttccgg ccatacttct tgtatgactg ccatcagctc atccagttca ttttctgtta
    31501 cttttatttt ttccatacac ccacgtcctt ttttacatta taaatgacct tgcgcttgat
    31561 ggaataaaaa gagcatgatc atgctataca cacccaccga ccacggtttc aagaaaatca
    31621 caatctgcat aaattcccta aaagataatt tcgtcaaacc agctactaga cagatcaagt
    31681 cgtctggcgc aaagggaagc agcaaggtaa caactagtac ttttttaata tctttcgttt
    31741 ttcttgtcag caattcatca aatttctgat acttttttgg cgataaaata agctggacaa
    31801 aggctacgcc atagtaacgg acaagcaaaa atccgattgc ttccccaatc actaggccaa
    31861 tacaactata taaaagccca aatccattgc caaacatcag catgcccgct actgaggaga
    31921 tccctcccgg caaaatagga atgatcactt ggatcacttg caacagaaca aatatcatca
    31981 ctgcatactt gccaaattgc tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc
    32041 catgatgata agcataaaga atcaacacaa agaaaaaaaa cagaccaata atgggcatga
    32101 tctgaagaca tttttgtaaa actgatcctt ttttagacat ctatattccc cacatctttt
    32161 ttcatttatt aaaactgcct c
//
LOCUS       DAFCHQ010000023        31696 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000023 DAFCHQ010000000
VERSION     DAFCHQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.023"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..563)
                     /locus_tag="LNC71_001153"
     CDS             complement(<1..563)
                     /locus_tag="LNC71_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBK6466238.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="LNC71_001154"
     CDS             complement(750..1400)
                     /locus_tag="LNC71_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466239.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LNC71_001155"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="LNC71_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBK6466240.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="LNC71_001156"
     CDS             complement(2912..3910)
                     /locus_tag="LNC71_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBK6466241.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LNC71_001157"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="LNC71_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBK6466242.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="LNC71_001158"
     CDS             complement(4926..5657)
                     /locus_tag="LNC71_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBK6466243.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="LNC71_001159"
     CDS             complement(5661..6926)
                     /locus_tag="LNC71_001159"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBK6466244.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="LNC71_001160"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="LNC71_001160"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBK6466245.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="LNC71_001161"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="LNC71_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBK6466246.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="LNC71_001162"
     CDS             12268..13770
                     /locus_tag="LNC71_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBK6466247.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="LNC71_001163"
     CDS             13772..14509
                     /locus_tag="LNC71_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6466248.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="LNC71_001164"
     CDS             complement(14680..15873)
                     /locus_tag="LNC71_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6466249.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="LNC71_001165"
     CDS             complement(15952..16158)
                     /locus_tag="LNC71_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBK6466250.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="LNC71_001166"
     CDS             complement(16151..17293)
                     /locus_tag="LNC71_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBK6466251.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="LNC71_001167"
     CDS             17805..18440
                     /locus_tag="LNC71_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6466252.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="LNC71_001168"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="LNC71_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18916..19032
                     /locus_tag="LNC71_001169"
     CDS             18916..19032
                     /locus_tag="LNC71_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK6466253.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="LNC71_001170"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="LNC71_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK6466254.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="LNC71_001171"
     CDS             21086..21253
                     /locus_tag="LNC71_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBK6466255.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="LNC71_001172"
     CDS             complement(21313..21666)
                     /locus_tag="LNC71_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6466256.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="LNC71_001173"
     CDS             22170..22589
                     /locus_tag="LNC71_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6466257.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="LNC71_001174"
     CDS             22615..22821
                     /locus_tag="LNC71_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6466258.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="LNC71_001175"
     CDS             complement(23049..23222)
                     /locus_tag="LNC71_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466259.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23198..23863
                     /locus_tag="LNC71_001176"
     CDS             23198..23863
                     /locus_tag="LNC71_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6466260.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="LNC71_001177"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="LNC71_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBK6466261.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="LNC71_001178"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="LNC71_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBK6466262.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="LNC71_001179"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="LNC71_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBK6466263.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="LNC71_001180"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="LNC71_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBK6466264.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /locus_tag="LNC71_001181"
                     /pseudo
     CDS             complement(29076..30241)
                     /locus_tag="LNC71_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(30257..30484)
                     /locus_tag="LNC71_001182"
     CDS             complement(30257..30484)
                     /locus_tag="LNC71_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBK6466265.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31558
                     /locus_tag="LNC71_001183"
     CDS             30968..31558
                     /locus_tag="LNC71_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBK6466266.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
BASE COUNT         9252 a         6465 c         5594 g        10385 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaa
//
LOCUS       DAFCHQ010000024        31234 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000024 DAFCHQ010000000
VERSION     DAFCHQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.024"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            184..1056
                     /locus_tag="LNC71_001184"
     CDS             184..1056
                     /locus_tag="LNC71_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6466267.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1053..3011
                     /locus_tag="LNC71_001185"
     CDS             1053..3011
                     /locus_tag="LNC71_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6466268.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3343..4194)
                     /locus_tag="LNC71_001186"
     CDS             complement(3343..4194)
                     /locus_tag="LNC71_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6466269.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4317..4784
                     /locus_tag="LNC71_001187"
     CDS             4317..4784
                     /locus_tag="LNC71_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBK6466270.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5047..5529)
                     /locus_tag="LNC71_001188"
     CDS             complement(5047..5529)
                     /locus_tag="LNC71_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBK6466271.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5629..6492)
                     /locus_tag="LNC71_001189"
     CDS             complement(5629..6492)
                     /locus_tag="LNC71_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6466272.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7090..7965
                     /locus_tag="LNC71_001190"
     CDS             7090..7965
                     /locus_tag="LNC71_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466273.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8110..8262)
                     /locus_tag="LNC71_001191"
                     /pseudo
     CDS             complement(8110..8262)
                     /locus_tag="LNC71_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(8530..9861)
                     /locus_tag="LNC71_001192"
     CDS             complement(8530..9861)
                     /locus_tag="LNC71_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6466274.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(10010..10723)
                     /locus_tag="LNC71_001193"
     CDS             complement(10010..10723)
                     /locus_tag="LNC71_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6466275.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            10898..12322
                     /locus_tag="LNC71_001194"
     CDS             10898..12322
                     /locus_tag="LNC71_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6466276.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            12621..13328
                     /locus_tag="LNC71_001195"
     CDS             12621..13328
                     /locus_tag="LNC71_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK6466277.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            13315..13638
                     /locus_tag="LNC71_001196"
     CDS             13315..13638
                     /locus_tag="LNC71_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK6466278.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(13727..14764)
                     /locus_tag="LNC71_001197"
     CDS             complement(13727..14764)
                     /locus_tag="LNC71_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6466279.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(15085..16068)
                     /locus_tag="LNC71_001198"
     CDS             complement(15085..16068)
                     /locus_tag="LNC71_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBK6466280.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(16483..17253)
                     /locus_tag="LNC71_001199"
     CDS             complement(16483..17253)
                     /locus_tag="LNC71_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466281.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(17246..18181)
                     /locus_tag="LNC71_001200"
     CDS             complement(17246..18181)
                     /locus_tag="LNC71_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466282.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(18497..20146)
                     /locus_tag="LNC71_001201"
     CDS             complement(18497..20146)
                     /locus_tag="LNC71_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK6466283.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(20263..22866)
                     /locus_tag="LNC71_001202"
     CDS             complement(20263..22866)
                     /locus_tag="LNC71_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK6466284.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(22878..23927)
                     /locus_tag="LNC71_001203"
     CDS             complement(22878..23927)
                     /locus_tag="LNC71_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBK6466285.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(23953..24267)
                     /locus_tag="LNC71_001204"
     CDS             complement(23953..24267)
                     /locus_tag="LNC71_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBK6466286.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(24283..24729)
                     /locus_tag="LNC71_001205"
     CDS             complement(24283..24729)
                     /locus_tag="LNC71_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6466287.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(24730..26652)
                     /locus_tag="LNC71_001206"
     CDS             complement(24730..26652)
                     /locus_tag="LNC71_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBK6466288.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(26864..27760)
                     /locus_tag="LNC71_001207"
     CDS             complement(26864..27760)
                     /locus_tag="LNC71_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6466289.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(27753..28433)
                     /locus_tag="LNC71_001208"
     CDS             complement(27753..28433)
                     /locus_tag="LNC71_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6466290.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(28434..29216)
                     /locus_tag="LNC71_001209"
     CDS             complement(28434..29216)
                     /locus_tag="LNC71_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466291.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(29209..30126)
                     /locus_tag="LNC71_001210"
     CDS             complement(29209..30126)
                     /locus_tag="LNC71_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6466292.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(30290..30613)
                     /locus_tag="LNC71_001211"
     CDS             complement(30290..30613)
                     /locus_tag="LNC71_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466293.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         9452 a         6347 c         5254 g        10181 t
ORIGIN      
        1 taactgtcta tataaaattg attacgttaa caataattcg tttcatgtga ctagatcata
       61 ctaggcatga aaaatctctg aggaacatgg tttccctagg aggtttttcg tgtctatttt
      121 tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg
      181 cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat tggtagatga cggtggacag
      241 gaaaaagtag ctattggaaa agggattgga tttgacagaa aaagaaatga tttgattttt
      301 gatcgtgatg tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat
      361 ttacttaatc agattgatga acgcttttta tttgcagcag aaacgatcat tgatcatgcg
      421 gaaactgttt tgatggaaaa gttgaatgaa catgtattga tcgctttgac cgaccacatt
      481 gctttttctg cggagaacat taataacggt atcgtcatcc gcaacaaact tttaagagaa
      541 atcgaagtgc tttacagtga tgaattcagt attgctcaat gggcagtaga gtatctgaca
      601 aaagagcttg ggattccata tacttatgac gaagcaggat atatcgcaat ccatattcac
      661 agcggacgaa gcggtcggaa caataaccat cgcagtatcc gtgaagtgac gattgtatct
      721 gacatcatcc atttagtgga atcagaattg gaaacagata tccattcaga accattttca
      781 ctgaattatt caagactggt gaatcattta cgactgcttc tacaacgaac ccatgcgcaa
      841 caatatgctg ttttagacac ggaaatcgta gaaatggtca aacgaaaata cccagaaagc
      901 tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg
      961 aaagaagaac tgggttactt ggccatccat atcgagcgtt taagaagcgc aatcgtacaa
     1021 acaaatgtta acaatcatga ggaggaaaaa aattgaaagc atatctacaa cgaatgggac
     1081 gttcccttca acttccagtt gcggtcttac ctgctgctgc tttactagta gggatcggaa
     1141 actggtgggc atcttatagt aacgacattg tcgctcattt tttacaagct ggcggtaacg
     1201 cagttttagg tcaattacct ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg
     1261 acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt
     1321 taaaaactga ttcagtagct actttattga atatcaaacc agaatcagtt gatccatctt
     1381 ttgctcagat cagtaacgta tttattggta ttattgccgg attgattgcg gcagctcttt
     1441 ataatcgttt ccacgaagca aaattaccga tggctttatc tttctttagc ggcaagcgtc
     1501 tagtgccaat cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat
     1561 ggcctccggt ttactctgct ttagtttctt ttgctaaatt cattttgagt ttaggctggg
     1621 gtggtgcagg tctttatggc ttcttcaacc gtttactgat ccctacagga ttgcatcaag
     1681 caatgaactc cgtgttctgg tttgacgtag caggaatcaa tgatattggt aaatttttgg
     1741 cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc ccaatcatga
     1801 tgttcgggct tccagcagga gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag
     1861 caaaaacagc ttccttgatg ttagcagcag cttttgcttc attctttact ggtgtaacag
     1921 aaccacttga attctcattt atgttcgttg cttggccatt atatgtcttg catgcaattt
     1981 tcactgggat ttctttagca gtgagtgcat tcttccattg gacagcagga tttgccttta
     2041 gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat cagccattga
     2101 tgttgatcgt ccaaggatta gtaacagcag tgatttacta ctttggcttt aactttgcta
     2161 tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc agatctttca gatgacgata
     2221 ctgcaacgac taattcagac aataaatatg cggcacaagc aagcaaaatc tacgctgcat
     2281 taggcggagc cgacaatgta acttcgatcg ataactgtac tacacgcctt cgtttgcaag
     2341 tcaaagatac aggcctgatc gatcaaaata aaatcaaagc cactggcgta cctggtatga
     2401 agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag
     2461 atgagatggc aaaactacat ggtggtgctg caacacgacc tgcacaaaca aacaccgtgg
     2521 ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga tatttacgct atcgcaaatg
     2581 ggaaactgat tccaatcact gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg
     2641 acggctatgc tgtattacca gaaaatggag aaatcttctc tccaatcgct ggtacgatca
     2701 caaacatttt cccaacgaaa catgctgtcg gaatccaaac agatgctggt atcgaagtct
     2761 tgcttcatat gggcatcaac actgttgact taaaaggcga accattcact ttatatgtaa
     2821 aagaaggtca aaaagtcgca agaggtcaat tgattgcttt agttgatctt gccgctattc
     2881 aatctgcagg caaaaacacg gatatggtcg ttgtattcac aaatggcgac aaagtccaaa
     2941 gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca
     3001 acaaagcctg agccatcgtc ttccccagac ttttttatcg ataaacaaac aggagctgag
     3061 acaaaaatct tgtctcagct cctttttccg aataaaaacg gcgttctcct gccaactccg
     3121 cggtaataag tccagccaaa cgtaacatat gaagagctat tttttatttg actcttctta
     3181 ttacttgatg cagaacagct tttttacaac ctctgataaa cggtgttcaa aaaccagttt
     3241 atcggtatga agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3301 catactcaaa actaatcggc tttttcacaa cctctttata cattattcct gatccaatat
     3361 ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt ctaaatcatc
     3421 agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa acatgtcagc
     3481 tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat cacttgcttc
     3541 aataatttct aactttttac ctgtcgcacg ctctaatgcc ttggctaatt cttcatagtc
     3601 agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta gtacttttgc
     3661 tgcagcttct gcatattcac gtttcaatac ccaacctgtt tttccatttc ctgcagcata
     3721 aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct ctacatacca
     3781 attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt cagtgaattg
     3841 gtgatctggc gctaatacac tgatcgagtg atcggcatct gcaaaacttg tataagcgat
     3901 ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg cttgacgatt
     3961 tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt ctttcattga
     4021 gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca gtgcttctcc
     4081 tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct tttttaagaa
     4141 ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat gcatatttgc
     4201 cactccctat tctttttatt tgttaagatt tacagctgta catttataaa ttacaccttt
     4261 atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga caactgatga
     4321 agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa atgtttcaag
     4381 atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat gcagcaaatg
     4441 tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa accggaaaac
     4501 cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc tatcgcagca
     4561 tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat tgcgtcgttg
     4621 gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa aaggcagaag
     4681 aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt ttggaaaaag
     4741 aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct ctactgtgcc
     4801 tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg gacaaaagcc
     4861 ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc atgacgtctt
     4921 gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc aaaatatgga
     4981 aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt cacgacctct
     5041 atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt tcccgttggt
     5101 aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt ctacttctct
     5161 taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag gcgattctgc
     5221 taaagcgatt acaacaagat acgttccttt tgggaatggg atgattcggg tagtgtttgg
     5281 aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat agggttcgtt
     5341 gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg ttccattacc
     5401 gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat ccgtattgct
     5461 actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt cgatcctata
     5521 ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa aattaaaggg
     5581 aaatgacacg attacgctct tttttctagg gatattttta ttttttaact atttttttgc
     5641 tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc attttgtaaa
     5701 agagatctcg ggagcttcga ctaactcata atctgaagaa ggaagccatt ctgcatagat
     5761 tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa aaatggccca
     5821 agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt ttgtcgtagc
     5881 aaagccaatc atgtgcatca aactgccctt ttcttccaag cgggctccat caaatccata
     5941 agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct cttccctttg
     6001 agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt caaatttgac
     6061 aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga tacgaaattc
     6121 catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag atttttgtat
     6181 gccgtatctg ctgatttctg aaggcagata tcctgaccaa tcacgaaaag ctcttgaaaa
     6241 accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg atccatctag
     6301 gagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta gcgacatccc
     6361 tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat tttcggctat
     6421 tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca attggttcag
     6481 atctttaagc atatttatct tcctccttgc ttgccttttt atcatattat caaagaaagt
     6541 caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg tatggatcta
     6601 aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc tatctatgtt
     6661 ttacctttag aaaaataaat tttggggaag acacattcta agtaaacaaa gcggtaaaaa
     6721 tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa attgttgttc
     6781 aaactgctga tcagaacgct gcttggattt ttctttgcga tacatcatac ctcttttatc
     6841 aattggaaca attatttatt tctaacaaaa tcacttcatt tttgtcagat aaataaaaaa
     6901 caaaaaaagt tccatcttta aaacacgcta ttcttttact ttttctcaca ttctctaaga
     6961 aaagaaaagg agaatgaatt tttgacaatt cgagcaaaag tgattatcaa atatgtaaaa
     7021 tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt ttcatgaaag
     7081 gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga acgcggttac
     7141 agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta cttgtccgca
     7201 acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa gaccaacatg
     7261 acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc tgctgtctta
     7321 ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga actctcgtcc
     7381 cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat tcttatagaa
     7441 catccgcaat ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat aaattattca
     7501 gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa caaaaacagg
     7561 aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt tgaatcgctc
     7621 attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat tgctactctc
     7681 gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga ggactgttat
     7741 tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca aatgttctct
     7801 tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta ctccataaaa
     7861 atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa aatagataaa
     7921 aaaactaaag aaacggtaaa gaaagacgat ttcaatgaaa aatagcaagt aaacgataac
     7981 aaccatccta aatccacaag aaatacagca gaccttgctt taaaaacacg aatcatgcaa
     8041 aggatgaagt aaagaagact taacgcgtac ccaatattag tcatttgact attaggtacg
     8101 cgttttgttt tataagtcat gacaaatttc cttcgttgct gggtagattt tccggcattg
     8161 ttgatagatt ttatgatact tttctacatt ttgtaagaga ggttccactg catctgtata
     8221 ttggataaaa ttctgacaac aactttcagg tgtgtcgaac cactctaatc tttaaagata
     8281 aatattaaaa taacgattaa taatattaac agtctttatt tttcaccacc aaaaacctat
     8341 ctttgcatat actaaataga cgtatataca cagataggtt tgtcttgtga aaaataatga
     8401 ttatccttac caaaaaatgt ttgtttttct acatgttcgt acttatcaaa aaatatgatc
     8461 cttattgaaa ttactggcta tttatattcc aacgacttct tgtgttaatt tttgttatct
     8521 catagtaaat tatgtttcat tttcacttaa tttagttgtc acgtgttcgt caattcgtaa
     8581 aaacggtaaa tatataaaca cacttgcggc tataattaat atttgtaaaa cagctgctcg
     8641 ccagtctccg gctgttgcta aaaatcccga tataactggt ggcgtagtcc aaggaatttg
     8701 cactactaca tgattaatta atcccacagc agttgcaaaa tatgctactg tcaaagaaaa
     8761 aatcggtgca agcacaaagg gaataatcag actgatatta aatacaatag gcaatccaaa
     8821 aataataggt tcgcttatat tgaatatagc gggagttacc gataatttag caaagtcctt
     8881 ataatctttt ctacgactga agataaatat tgcaatcaat aaagcaatcg tatttcccga
     8941 tccacccatt accgcaaagg tatctttaaa ggccatattg ataatatgtg gaatttgctc
     9001 tttgtttgca taagctgcca tattttcttg catattctga atcaaaaacg gatctaacaa
     9061 tgaacctgat attactgctt gatggatacc aaatccaaaa aatagattag caatagatgt
     9121 aattaacaaa aatcctggta aaccagttgt aattttactt aatggctctg taactatcgt
     9181 ggtaattagc ttatttatat ccatattaaa caattgaatg attataaatg acaataaagc
     9241 aaacgaggta atcgtaataa tgattgggat taatacatta aaagacttta aaacagcggg
     9301 aggaatattt ccggatagct gaatctgtag tttcttatta ttagaaagtt taataaataa
     9361 ttctgtagcc gctaatccaa caaatatacc tacaaacatt cctgaagctc ctgtatagga
     9421 tataggtatc acacccgtca cttcaacagc tttatccgca ttttcaggag taaacatcat
     9481 ctgcataggt gttaatacaa caaatgtcga aatagcaact aataccgctg caatatgatt
     9541 tgtataattt ctgcttgcac acatatgata agatactgct gctacaacta ataatgtagt
     9601 tatgcttaat gtaccattgg caattgaagt accaatttgt tgtaacgtgg tcaatgtact
     9661 agtatcgaca atttccccca tgaatcctgt tggttctaag atcactgaat taataagtgt
     9721 cacaaatcct gctaacatca taaatggcat aaatgtagca aacgcatctc gcaaagttct
     9781 taaatggatc tgattcccca acttagtcgc aactatacca aatttttcaa atacaatgtc
     9841 cttcctgcta cttttgccca tattttttct cctttatgct tattgtaatc gattactttt
     9901 tccacccaaa taatacatca cttatcaaaa aaagtaaata cttttaatta aatatatata
     9961 tcttttttag tataaaaaaa taagctctga aaaaataatt ttcccgagct tatttttcaa
    10021 caaaaacagc ttcaaaaaca aagtcttgat ataaatgtct cgataccgaa tactcgaaag
    10081 gtttaccaga cgatagagca acaactttgt ctatctcaat cataaattca cctggatcta
    10141 tttttaatat ttttgcgtca aattcattcg cagtatctcc tttgatacgt atgtgcgaag
    10201 tctttatttc cagacccagt tcatttgtga tataagcata tacagatttc tttaaatgtt
    10261 ttggttccaa gcctggtatg actgctaatg gcatgaatgt ttgctcaatc gagtatggtt
    10321 gacaatcaat aaaacggctt cgtacaatat tatatacata gtcttttaca ctgatactta
    10381 atttatcagc tacttttttc gatggaagtt caattgagaa agtatggatt tctgaatcta
    10441 cgtgttcttc accaaccacg ctaaacaaac cttcgtttgg accaagaact cgtgagttat
    10501 caattggtgt tctgacgact tcagtcccat acccacttct acttcgcaaa actccttcgg
    10561 aaacaagtaa gtctacgcct ttctttactg ttaacttgct tgtatgataa ataactgcca
    10621 attcatctcc tgtaggaagt ttctcaccaa ttttatactc tccatttaca atttttttct
    10681 ttatatctaa gtaaatttcc atatacttcg caattttttt caatggtgac cctccaatca
    10741 aaaacatatc ttcatctata ctttaacata aataaaccga taaaatccac atcaaaatta
    10801 tacacacaat aaaaaagata tatatattta aacaaaacta tttactttaa gttgtaagcg
    10861 gtgttatcat taagtggaaa caggaggtaa aaaaattatg acaactatga aaaagctaac
    10921 ttttccagat gatttttggt ggggttccgc ttggtcagct gagcaagcag aaggaaaagg
    10981 agaaacagga aaagcagaga ctatctggga tcgctggttt aaagaacaac cttaccggtt
    11041 ttatcaacga atcgattcct ctattacaac cgatcacatc catcaatata aagaagatat
    11101 agaactaatg aaaaaaactg ggcataactc ctttcgcgtt tctatttctt gggcacgaat
    11161 gtttccaaat ggcggtaccg gaaaagtaaa tcccaaagcc atttctttct atcgaaaatt
    11221 attttccgaa atgaataata acggaatcaa agtatttgcc aacttatatc attttgacat
    11281 gcctgctact cttcaagata aaggaggatg ggaatcacgt gaagtagttg atgcttatgt
    11341 tcattacgct gaaacttgtt ttaaagaatt tggtgactta gtttgtcatt ggtttacctt
    11401 caatgaacca cttgggccta ttttaggtac ttatcttgaa gattttcatt atccaaacct
    11461 cattgacttt aaacgcggag cgcaagcagc attcttcact atacttgcac acgcaaaagc
    11521 aatacaagta tttaaagaac tagctcttcc tagtaaaata ggcgttattc taaatttgag
    11581 tcctactttt cctcgtagtc aaaatcctgc tgatgtgaaa gcagcagaaa tcgctgattt
    11641 attttatacg cggagctttt tagatcctat ggttaaaggg aatttttcta aaaagcttat
    11701 cctgttatta caagaatata atcagcttcc tgaagattat acgaaagaag atcttgtatg
    11761 tatctacgaa aataccgctc aaattcttgg actaaactat tacgagccac gtcgcgtcaa
    11821 atctcgttct tctatggtgg atacatctag tccattttta ccagagtggt ttttcgaacc
    11881 acatatcatg cctggacgac gaatgaacga ataccgggga tgggaaattt acgaaaaagg
    11941 aatttatgat ttgtgcatgg atatcaagaa ccattatgga aacatcgaag catttatctc
    12001 ggagaacgga atgggcgttg cggatgaaga acgtttcatg gatgatacag ggcaaataat
    12061 tgatgattat cggattgagt atatcaaaga tcatcttgct tacttatgga aggcaataaa
    12121 tgaaggatgt aacattaagg ggtatcatct ttggacattt attgattgct ggtcatggat
    12181 caacgcctat aaaaaccgat atgggctggt ttcactcgac cttactactc aaaaaagaac
    12241 aattaaaaaa agtggcgaat tttacaagca attaagtgat acaaatggat ttagatacga
    12301 tgtagacttg ttagttgaat aatattctaa aattccactt gaagtgaaca cattagaaaa
    12361 caagaataat gagaaatacc aataacaaat actttttctt catacttcat ttatacttgt
    12421 cagtataaga gactgtgaca aatgtagtga tcctcagaag ttaggtctgg aaatctaact
    12481 tgagaagatt tttttaaact acgtccagtc ttttttattt gtttaaaatt gaagttgaca
    12541 agtcttcatt aaaaatttag aatattcaca agtttgacga caattatgaa aatttatgtc
    12601 gtgctaaagg agttttgtta ttggatgaaa agttagatat aaaaactgcg atcaaagaca
    12661 cgttacctac cgtttttggt tatatcggta ttggacttgc atttggtatc gttgggaaag
    12721 ctgccgggtt tcatccgctg gtcgtcacgt tgatgtccct gctgatctat gctggttctg
    12781 cccaatttat cacggtcagc atgcttgcta gccatagtcc actgctctcc atcgttctct
    12841 cgacctttct agtcaattcc cgaatgattc tgatgagtat gacgattgct ccttatttca
    12901 agaaaaatcg gctgcttcaa aatctgctga ttggcacatt gctgacagat gaaagtttcg
    12961 cactgggaat gaataaattg aactatactg gacaaaagtt aaactttcgt tggatgaata
    13021 cagctaattg gatttcttat ttgacgtggg tgggttcttc tcttgttggc gcacttttag
    13081 ggaattttat tactgatccg aaaaagtttg gtttggattt cgccatcgtc gctatgttta
    13141 tcgggttact ttatcttcaa gtcatttctg atagaaacac ctctaaacga ttacagttga
    13201 tcctgattgg tctcactttg attctggtat atgtcggact gatttttatc ccaagtaact
    13261 tagtgatttt agttgtaaca ctgattgggt gcggattggg ggtgtggatc aaacatgcct
    13321 tcttttgagt ttacattgct gactatttta ggttgtactt tagcaacttg gctttctagg
    13381 atcctaccat ttgtcttact gaaaaaattc gatttgccgc aaccgttgct tgagtattta
    13441 agttttgtcc cgatcgtcat tatgtctgct ttatggttca gcagtctatt tacccaaaat
    13501 atcggacatc tgccgcagat caatctcgaa aatgctttgg cttcagttcc tacactttta
    13561 gctgctattc tttcaaaaag cttattggtg atcgtcttgg taggaattct ttccctttcg
    13621 ttgattcgct tattataaat aaaaaaacaa gagcttatgg caaattactg tcatgcgctc
    13681 ttgtttttat caaagtcaaa taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg
    13741 gaccactaac aacgactttt cctaatatat gtcctgtttc tacgtctttg gttgcttgtt
    13801 tcaagttctt cgcattcatc ccatctgaat agaccttgct caatgtactg cggataatcc
    13861 cttcatttgc taaagcagca atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt
    13921 tatagtctgt tttagcgaac atatactccc agtcgaagct gatagattgg tttttccaaa
    13981 cacctatatc taacggctct ttgatcccta cgatcgttcc cacatgtcca aatggtcgga
    14041 caaggtgttt gatttgatca aagtagcggg taatatcgaa taatacagcg acatactcta
    14101 cttgatcgat tcctaattca cgaaaacttt tacttaagtc gtgatgataa tcgattggat
    14161 agtctacacc tgtttttttc agccattcaa aattcttcgg acttgcagtc gcgtagactt
    14221 ctaggccagc ccaatgcgct agttgattca aaatcgaacc gacgccccca gctccgttga
    14281 tcactaagat ttttttcccg cggttggcat tttcttctgg agtcagacca aatttttcaa
    14341 ataacaattc ataagcggtc aatgaggtca acggcaaggc tgcggcttct tcatctgaaa
    14401 gattttttgg tgcaagagcg acaatacgtt cgtcaaccag ttggtattct tgattgcttc
    14461 ctgctcttgt cgttgtacct gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt
    14521 catcacctac agcaacaact ttcccgactc catcaaaccc taagatacgt aaagcatttc
    14581 ttatcccgtt atcttggcga agtttcgtat cgacaggatt aacagatact gcgttgactt
    14641 ttaccagaag atcccgtgaa ctgggtacag gtatctgttc tttttcatca ataaagcttt
    14701 ttggatcatc gatcgacaac ccttcataaa aaccgactac cttcattaat tccttttctg
    14761 acatacgaat tcctccttag cttcatcaag ttggagcatc catacagttt tcatttcaat
    14821 atatatagta tgctatttgt tccactttta ttcatcataa ttattctctt acttaaagta
    14881 aactatttgc acttttttgg ttaaaaaaga ggttgcgaca agacttttgt cacaacctct
    14941 tttaaacggt gttcaatcaa ctgaccctcg gtattcgctc aaaaaacgac aaaatatgag
    15001 gagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacgacctct
    15061 aattcacggt gttcaagcaa gaaatcattg ttcactgaaa agcgttggat gttctttttc
    15121 aaaggtgtct aagatctctc gtacttcatc agtagatttc gtattcatca actgaactcg
    15181 caagtcattt gctcctggaa atcctttgat atagatttta aagaaacgat gtagtcctgt
    15241 gatggaacgc gggatgattt ctgaataatg atcttgcaga tctagttgca gacgaagtag
    15301 tccgatcagt tctttttctg tatgttccct cggttctttt tcaaatgcat aaggattctt
    15361 gaaaatcccc cggccaatca tgatgccatc tacgccatat ttttcggcca attcttctcc
    15421 tgtctggcga tctgggatat ctccgttgat cgtgatcagt gtttgcggag cgatttggtc
    15481 acgtaaagca atgattttag ggatgacttc ccaatgtgca tctactttac tcatctcttt
    15541 acgcgtacgc aaatggacag aaagattggc tatatcttga tgaagtaggt gactgatcca
    15601 ttcttccatc tcagacattt ccgtatagcc gatacgcgtc ttgacgctga caggcaaacc
    15661 gccagcttta gccgcttcaa tcaattgtgc cgctacttct gggcgaagga tcaagccact
    15721 gccttttcca cgttctgcta cattagggac tgggcagccc atgttgatat cgattccttg
    15781 gaagcccatt tctgccatag caatgctcat ttctctaaaa aattctggct tgtctcccca
    15841 aatatgggca accatcggct gttcatcttc tgtaaaggtc aatcttcctc gtacactgtc
    15901 ttttccatct ggatgacaga aactgtctga gtttgtaaac tcagtaaaga agacatccgg
    15961 tgctgctgca tgctttacga catggcgaaa tacaacatct gttacatctt ccattggtgc
    16021 taaaacaaaa aaaggctttg gcagctcttt ccaaaaatta gtagtcattt atttgttact
    16081 ctccattcta tcaagcagtg aagtcgcaat ctttctcaaa aacattgtca ttatactagg
    16141 aaacaaaaaa agaagcaaag tgaacagctt aaaagatgtt tctgattgag caaaaaaaga
    16201 ctgtgacaaa aatcttatca caattccttt tccggaataa atggtgttca aaagcttgca
    16261 tctgcggtaa taagtccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
    16321 ttattacttg atgcagaaca gctttttcac aacctctcca ctaaacggtg ttcaatcagc
    16381 tgaccctcgg tattagctca acaaacgaca aaatatgagg aactattttc ccgtttgttt
    16441 ctctaatact caggtcaaaa cgctgatttc acaacctctt ttctaacttc tcaatcctct
    16501 tcctttattg atcaaatacc aagtaacggc gtacaaaaca acgatgaata gcacaagtac
    16561 gatcatcgat gttgctactg gcacatcgat tgttccaagg aatccgtaac ggaaaccgga
    16621 gatcatataa acgatcggat tgactttcga aacagcttgc caaaatggcg gcaacataga
    16681 gatggcatag aaaacgccac ctagataagt taacggctgc aagacaaatg taggcacgat
    16741 cgatacatcg tcataagact gagcaaaaat cccgttgagc agtcctgcta acgaaaacaa
    16801 gattgccgtc atcaataatg taatgatgac catgaaccat gaatacacat gaagtggtac
    16861 aaagaacaag gaaatgatcg ttacgagaga gccgacgaga atactgcgac ccactccgcc
    16921 aatcacaaag ccccaaatga tcacatgcgt aggaacaggc gctaccaaga tttcttcgat
    16981 gtttttctgg aatttttgag agaagaagga cgaagaaacg ttggcatacg aactagtgat
    17041 agcagacatc ataatcaaac caggcacgat gaattccata tatgaaaaac cacccatgtc
    17101 accgatccga ccaccgatca tttttccgaa aataacaaag tacaaggatg tcgtgatcac
    17161 aggcggaacc agtgtttgga cccagatacg caaatagcgg ttcgtttcct tgatagctaa
    17221 actttttaat gcagtaaaat aaagattaaa catttttctc ctccgtatga ttattttctt
    17281 ctgtgatttt caaaaacagt tcttctaaac gattcgactt attcctcata gaaagaactt
    17341 tgattccttg tcttgtcagc tgatcgaaga ttccgttcac cccttgattt cgttcaactt
    17401 cgacagcgat tgtctgttca tcttcaaatg tacaagcgta tccttcaatc acaggtttgg
    17461 tctcatatgg cgctaaatca aagataaatg tttcaaattg cagtttggat agaagctctt
    17521 tcatactagt attctcgatc agttcacctg attggatgat cccgatgttg cgacaaagca
    17581 tttcagcttc ttctaaataa tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt
    17641 ctcggagaaa ctcccacatc tcgcgtctca attcaatatc gactcctgct gttggttcat
    17701 ccaagatcag caaacgtggt tcatgcatca atgccctagc aatcatcagt cttcgcttca
    17761 ttcccccgga aagcatacgt gcacggacat ttctcttttc ccaaagattg gactgcttca
    17821 aatatttttc gctgcgtttc atcgcttctt tgcgagagac accgtagtag cctgcttgat
    17881 tcacgacgat ttgctggacc gtttcaaagg gattgaaatt gaactcttgc gggactaatc
    17941 caatctgttg ctttgctcgg actagatccg tatccagatc ataaccgaag acattcactt
    18001 ttccagatgt tttattgact aaagaagtaa tgattccgat cgttgtagac ttcccagctc
    18061 catttggtcc taatagcgca tagaaatctc cttctttcac ggaaagatcg attcctcgca
    18121 atgcttcgac cccacttcca taaacctttt tcaaattatt tatttcaagc gcatttgcca
    18181 tttatttcct atctccttat agacatgatt ctttattgta tcatggaata cagcagatga
    18241 aaaaacaact tggttccagc gaaaaatcag gttgctgggc tcaggaaaat ctcctaatga
    18301 aagacatcaa tcggacgaag tcagacaacc ttttcagaaa aaacaaatcg aagataaagg
    18361 attactattc atatgtgcaa caaaaggagc gtgagacaaa agtaaagaac acttttgtct
    18421 cacgctctaa acacgtataa acggcggaag caaaagccaa gatttttgat ccaataaata
    18481 ctttcgatca cttcgcctat cccaatacat gccactgccg cttttcatca aataacgcgg
    18541 cttccgtttt tgcggtttct actaaagtct ctgggtaatc caaaactttc agcaaggcaa
    18601 tcgcatttcg ggtccttgaa ggtccttctt ttaatttgaa atcaaaactg attccttttc
    18661 cttctgttac ttgttcttca aagtggacat tctcacaata attctttaaa atctctgtca
    18721 attcgatatc atgtgtggcg acaaatgcta aagatggata ctccgcaagc caccgaacga
    18781 ctgaagcgga agaagcaatc cgttcgatcg tatttgtccc ttttaaaatc tcatctacga
    18841 agcagtagca gcgttctttg gaagccactt ggtccaacaa acgtttgatc gatttgattt
    18901 ctgaaacaaa ataactgtcg ccttcaaata aatcatcttc gattgccatg gaagtcaaca
    18961 catgtccagg ttgcagggta aactgttctg caatcgctgt ttggatcgtc tgtgctagaa
    19021 tacagctgat ggctatactt ttgacatacg tagattttcc tgatgcattg gaacccgtga
    19081 ctaatgtatt cttctgccag ttgaccggat tcactaccgc ttcttttaac aatggatgat
    19141 agatatcttt cgctgtcaca ccgccctcag taaactctgg tcggcaagtg atgggcatat
    19201 aagtacggta attcaatatt gcagcggcta cttctaattc tcccagcaat tcccacagct
    19261 tgatggcttc agttgaatgc ttttctaatt tagtgagaac gaaatgatag gagataaatg
    19321 gcagcatgaa catgatgttg acatattcaa aaatcaactc tgcttcactc ccactcttca
    19381 tacgaaatga aaaacctaat ttagggatgt ctcttaatgg tttagtttgc tgtttcagct
    19441 cttcttgaag tggtgtcggc agttttgcta gctgattggc ggctgaaatc gttgaaacca
    19501 aataactcat gctattcagc tctgtttcta gtgtttgttt cttcactaga taatagaccg
    19561 tattgaatac taacccaccg attgccaaat agattcctag tacttgtcct aaaagaagca
    19621 aacccaaacc caccagcgga agcaagccaa gaaaaataaa taaacctacg cgtccaatct
    19681 ttttactttt tccatccgct agatattgtt ttgcaaaatt atgatctttt tttcctaaac
    19741 gagcgaattg atattggatt ttttctctca gttgaggatt ttcctgataa aaagcaatca
    19801 gtttttctaa ctgctgatct tcaccaaaat caaagctgcg taaacgttga tacaacgctt
    19861 ctgaacctac actagaatag gtactgttga ttccttcgaa tacttcgaac atatccaggt
    19921 cataccaagt aagatcatcg atttcgctat cccatgaacg gaacttcttc tccgtgagcc
    19981 atgcttcctt caagctttct tctttatcaa atctcggttg ataaggaatt ttttcccatt
    20041 tgctacgaac tgtttccttt aatttcaaac gtgaaaaaat gtctaaagca actaataaaa
    20101 taacagctgc aataataatt aaaacgataa attctctttg acccatatcc ctctccgttc
    20161 ctttatttgt tagctttagt atagcactgt tgcgctattt gaaaagaggg taaaaaaaca
    20221 cctttcaaaa taaaaggtgt ttttttcttg tatattacat atttatctct catttatcaa
    20281 aattgacaaa taatcatagg gaggaattgt tgttaattct tgtttattct ccagtgcatt
    20341 gacaactgtc ccaagggaaa ggatagcctc aggtactatt ttttcttgtt ctgttggatt
    20401 agctaagcga agaacaaatc cttgttcatg aaaataagaa ggataaatcg atgatacaat
    20461 taagtcttca ggtaattcaa tcaatgaaaa tttacgagga acatcaagtt cattttgtaa
    20521 tggccaaatc ttgttatcta acctgtgaat aaatacattc agtttctgct tttgataaga
    20581 aatagattgt tctaaccgtt tttctgcctg ttttgccact ttgaactcat taaatcttcc
    20641 ttcttctaca ttaaatccaa aagtgacttt atactcacct atctcttgag ctagcggtgt
    20701 ttccatcatg acatgccctt catttgtcgt atctcctgaa gctcgcccag gtcgccaagc
    20761 aagattaggt tttccaagtt cccctgttgt tgaaaataag gttatgatta attgattttc
    20821 tatacgttca tactctttta aaccatctgc ataaaacgta aaataatgat cattttctgc
    20881 cacagagaca ctcttatcga atatttctaa attaacaggt ttctctacca tagtttctga
    20941 ccaattcgga ggaatgacat caggggtatt ttcaataaat ccgttttgaa tttgtgcaat
    21001 tgatttttca gatttatcta aaacattaat tgctaagcgc atacgatgcg agtaaacagt
    21061 attatgaatt ttgagtgtgc cttcaacgat ttgactttct cccagactga acgatatttc
    21121 ataagatatt tcttccattt cgggtttttc agataaacga tcttttaaat ctttaggaag
    21181 ttgagctttt ccgtaaacaa ctaatgtttc ttgtaaagaa tctttatata ctttagctgt
    21241 ttctaatttt agagatagtt ctgtatctcc ttctaatggt gaataatcat acgtatcgcc
    21301 atcatttgct gaatcgatca aatgaacgaa atcaagatat tgtctatctc caactatcaa
    21361 attcaattgc ccatctttaa aagaaagttt atatttattg ttacttatct cagttttgtc
    21421 tatctcgatt gctgtttcta atttttcatt atttttctca aattcaacaa cagtgtatcc
    21481 tagtgctggc acatttacat ttatttttat gtcaagttca taataagctg gttcatcaaa
    21541 atattcgaaa cctgtagcaa tcatccgata agcatttttc cgttctggaa aataaacttc
    21601 tttttcaatg atattatagg gactaccttt gaagtgtatt ttctttgaat cagctactac
    21661 atgcacgatt ttcgatccac tataattctc agtcgttgtg ttgaaaacaa ggacttcatt
    21721 cgattttagc tttaatgcat cagccatccg ttttacaata aggttttcga ttccgtcagc
    21781 tatctcatta gcttctttga ttcgatggaa aatatcttcc gcaacattat ctgaaacaca
    21841 gccaccaata ctatcatgag cctggttttg caaaatcttt ttccataacc gacgaagtag
    21901 gcctttactg atatggatac catttctttc tgcaatcagc atgagcggct caactcgttt
    21961 tagaattttt tgttccaatt taaaattttc tattttcatt tttgttctta ctgagccaat
    22021 agaacgatga acacgagagt aaaccggctg acgaagttca cctttatatt caggtaattc
    22081 ttttttgttt ctgataactt ctacataaga aggataatct gaaataatat tgtggaattt
    22141 actgtattta ttcaacttct ctacgacttg atcaaaattt aaaatcattg acttttgatc
    22201 tacacccgaa ggtactaaaa tatctttgtt ttttccttgc atatgaagca tttcaactgc
    22261 ttcatccaac ttttcttttg caaattcagg aatatcttta attgcgtcta aggtcatcat
    22321 tccagtcata taaccaaatg gaaaattcat ggcataaaca aatttatctc ccaatccttt
    22381 ccagataaaa taaggagatg gaaccatcgt ttcaaagttt aatcctcgcc aacacatgaa
    22441 attatcgatt tctacttgat gcaaaagtgt aggcaaattc gcattgaacc caaacgtgtc
    22501 aggcaaatac cctaccatca ttggatctcc atatttaagg atcgtatcac tgattccaat
    22561 cattaagttt cttaaaattg attcagcatc cactaataag gcatctgttt gtgcatacca
    22621 cggtcccacg aatagttgtc ctgttttcat cagtcgttgg ataaccttaa gcttttgcgg
    22681 attgatctcc aaatactcat cgacgattga agtttgtcca tctaaacaaa agtttacttt
    22741 agggttcctt tccaattcat ctagaacttc agtaaagacc tgatcagaca atacgatggc
    22801 atcttcattt gtaaaatacc attctctgtc ccagtgcgtg tgttgaatta tatgtgcatt
    22861 taccatatca gattctccta ctttccttct tctactgaat ttacttgttg ttctttttta
    22921 tacttgatgg attttagtgc attgaccatt aacgctgtaa ccactccccc aataacaatt
    22981 gatactaaat ataaaggtat attcgtaaca aaaggaattg ccataaaggt tgaaagcatt
    23041 gtttcttgag taacaccaaa aaatccattt agaccacatg ctactgcagt gcccaccacg
    23101 atgcttggta ttactcgtaa aggatctttc acggcaaacg ggatcgtccc ttcagtaatc
    23161 cccacaaggc ccataatcat agctgtcttt gcattatctc gttccgattc atcataaagt
    23221 tttttgttta aaattgttgc taaaaacatt ccaaatggcg gaatagcaat tcctaataac
    23281 atggctgtac taggtccaaa gcttccgctt gaaaaagaag caaccccaaa agcatatgcg
    23341 attttattga taggtcctcc catatcaaac gccatcattc ctcctaaaat agccgacatc
    23401 aaagcagcat ttgctcctga catgccttct agccaattag tcagtcctgt tgtcattgct
    23461 tgaataggcg tagctagtac ataccataat atgattccaa caactaatgt agtaacaaaa
    23521 ggaatcagca taattgattt tagactaata acttgcatcg gtacttttac ttttttcaat
    23581 gcattagcta aatagccaca agctaatcca gcaaccattc ctccaataaa tcccatacca
    23641 agattttgac acatcatccc agtgataaaa gctggtgcta gaccgggacg gtcagcaatt
    23701 gaatatgcga tataggctga aatcatcggt acgataaact gtaatcccgt ctgaccaatc
    23761 attcgcagga ctccccagaa tgtatccgtc tggtcccaaa cacctgtgcc accaaaaatg
    23821 actgcaatag aagttagtaa ccctgctgct acgacaagcg ggatcatata ggaaacccca
    23881 ctttggaaat gatttagaat ttctcttcct tgtttcttga attccattag taactccccc
    23941 acgatatatt ttttatttgt taattaatgc aattgcttca tcaatcgtct cttcaccatc
    24001 ttttactacc tctgaggtcc ctactgttaa aatcggttta ccgtcaaagc gacctcgttc
    24061 tgaaacattt gtatctactg caaatatgac aacatctgcc tcggaaacat ccgaaattga
    24121 aagtttgttt tctatcccca tagccccctg tgtttctact ttaatatcta accctttctt
    24181 cttcgcaaac ttttctaaat tagcttttgc catataagta tgggcaatcc ctgcgataca
    24241 tgctgttact gctactattt ttgtcatatg aaattcctcc ttttaaaatg atagagcttt
    24301 taccaattct tttttatttc tcgaatcttt tattctttta acaaactcgt catccatcaa
    24361 tcttccagat aattctgcta aaatacgtaa atgattagta cctttatcag tgtcttttat
    24421 tgctaacaaa aagacgagat caactggttg atcatctaat gcgccccatt caacagaccg
    24481 ctttaacttg gctacagcca ctgttgattc aattactgct aggcttttgc catgaggaat
    24541 ggcgatattt ttcccaattc ctgtggtcat atggttttct ctgtcaaata cgtctttttg
    24601 aaattgtttg tggtcagaca caaacccatt agtctctaac aatttcgata atttttcgac
    24661 tacacttttt ttatcagatg catttaaatc tatcactata ttatttatat tgattttttc
    24721 actgatcatt tataagcctt ctttcaatat ataaactaat ttttcaaacg acgaactcgt
    24781 ttttaaattt gctaataact catgattcga agcgatttca ttgaatgttt cataaatttt
    24841 attcaattca ggttttactt tttcattaag tgcaaaaaag aaaacagaat gaacaagtgt
    24901 cccatcccaa tcaattcctt ctggattaac gtagacacag attactggct tttttataaa
    24961 atctacttcg atatgcggca tagcaaaatc attcatcgct gttgaagata gtttttctct
    25021 tttgattgct ccctctagta tcccttcttt cgcgtaattt tcttttacca attttttggt
    25081 aattatattt aaaacgttca cttgattatc ctttatttgg tgggtaaaaa agcaaaatcg
    25141 tttatctaat aaatttagaa aagcatcttc tgtttgttca tttaactgta gatactgttt
    25201 tacttttctg acatctgccc ctaacatcaa aggactaaca acaataacag gaaaatttgt
    25261 attttcaatc ggaatcgtgc ttataacaag ttcatcagtg acttttctat cttgtagttc
    25321 attgatgctt agtacatctt ctatctcaac catatctgca aaaccgttat ttattctttg
    25381 ctctaataat tttgatgttc catatccgct actacaaact aatatcactt tcgttttgtc
    25441 aggtttgtaa cgatccaaaa gtgattgaat atggagtgtt acgtaagcga tttcatcttc
    25501 attaaaatgt atacagaatc tctcttcgat ttcctctaat aaatctacac ttatcataaa
    25561 tgacctttta aaagaatatt tgattttgtc cgtataaggg ttatagatat ttactcccaa
    25621 tttgagtctt ttaactgctg cattgagatg taatactaga ttattgatca attcttcatc
    25681 aggataaaat tcatttaatt taattcttac taactcttgt agctcaacac ccatatctaa
    25741 ataggaaagc ttcgtttgat ctaagtcgtg attcatagca tttttttcaa tcgaagtaat
    25801 gtgaatgtca acataagctt gttcatctat tggcacaata attgaaaatg ctttttcaat
    25861 cattttgatt aaaataagtg tattctttga gatttcttct tcagaattat tactacgtga
    25921 taagaaattg cttgtttcaa tcctttctat cgcaatagca aggtggatag ctaaagtttg
    25981 aaattcataa tctgtaaatt tcaattgtgt cttattcatg tatcgattaa gcaattcggc
    26041 tacttttttc aatatgtcct tatttaagaa attattcgct tcaccaatga aattgatatt
    26101 caagtctaag agatctgttt tactgttaga tgctgtaaag ccaccccaat aatagttcac
    26161 aacttctgac atcaaccttc ttttactttt ttcatcagct ttgatgcgca cgccatttcg
    26221 atctgaaact aaggacatcc catgcttgtt tagtcttttt ctaacatctt tcattgtgct
    26281 ttctaccgtc gacctgctca cgtataattc gtcagcaaga ttttgaatct ttagagaatc
    26341 attagtattc aataatttag caaacacata agttagacgg tcctccggag agtctgcgat
    26401 attgatttta tgactggata ataaatttcg aagtgtttct ttttttccaa taagcgaaac
    26461 gcctttttta ggttgaacca acatttgtac atctggcatt ttttggatta tttctttcaa
    26521 aattaaaata tctttgctaa ttgttttcgt agatacatat aattgttctg ataattcttt
    26581 gtaagttgtg tgctcatttt caatcaaaaa gtatagtatt ttcatttgcc gttcgctaaa
    26641 ttcgatgccc attatcttca cctcctgaat acagcttaag tgtacaatta ttatctctat
    26701 tttcatacag aagaaagttc ttgattaatg tagaagtttc taatattctt gattatttca
    26761 aatcaataaa aaagcctgca atatttatat cacaggcttt tttctctatt aaatatatat
    26821 aagataatac atttttctac tagttctcac taatcttgta taattaaacc ctccgcaact
    26881 tgacggagat ggagaagatg cccccgtcta atctagcggt catctctcct cccattcctt
    26941 ccaccaattt ttttgacagg taaagcccga ttccgctaga ttccgtattt gtgcggctgg
    27001 tattttcggt ataaaaacgc tggaagactt tttcaacaga ggtttgcgtt cctggtttga
    27061 taggattggc cactgttaat cgaatgaatg gatcctcttt tgaaaatgtc acctttgctt
    27121 gtccatctgc ataacgcaat aaattctgta tgatattttg cagcactcgc agcaacatga
    27181 gcggatctgc taatacagtt gtatttatcg agtgttcagc gaacgctacc tcgatatttt
    27241 tctcagaaaa ttgttcataa aaagagaaaa atacttcttc cacctcttta gaaaaagaaa
    27301 gcgtatggag atccatttct ttttgcttcg tctctattcg agcaagatca taaaactgat
    27361 ccgtcatact gagtaatctt gcaacagatt gtttgatgat cgttaaatac ttctcccgtt
    27421 tctccgtatc ctctacctgt tccagtaatt gtacataacc gttaatagca gtcaaaggag
    27481 ttctgagatc atgagaaagg ccgacgatcg atagtctgat gttttcttcc atttcttgaa
    27541 atgcttgtcc tttttgttca ttttcatcca accatgtatt cagtgcatct actaggtcca
    27601 ttagattctt ttctcgaaat tcgactgaaa gacgaccacc atattcttgt cgttcagaaa
    27661 gagtggcgat tttcttagtt aatctcttga tttccctttg caggaagaaa ataaaacttc
    27721 ccataaagat aacaacgaga agcaacagcc atttaatcaa atctaaaccc cactccccac
    27781 accgtatgaa tatacacagc atgtgccttt gctagcttcc ctcgaagatt ggaaatatgc
    27841 atattcactg ttttctcact atcaaaatat ggttcttgcc aaactgtttc ataaagattt
    27901 gctcgcgaaa atactttttt cggatgttct aaaaaaagct tcagcagatc gaattctctt
    27961 cctgtcaaat ggacagccac accattcaca tttacatccc tcgactcttt gtccagtaag
    28021 atttccttgt aatagatgcg ttgcgacaga tcttttactt ggacagaagt ctgatgacga
    28081 agttggatgc cgattcgtaa caaaagttct tcgatatcaa atggtttcgt cacatagtca
    28141 tccgctcctg ctgccaacaa ggaaacttta tctctttgat cgctttttgc tgaaagaacg
    28201 atgatcggaa cattcgatgt ctgtcggatt tctgctaaca atgcttcacc tgaaagccct
    28261 ggaagcatga gatctagaag gatcaaatcg acaggatatg tgcttaacaa taattttcct
    28321 tccgtacctg aataagcatc aagaagtttg tatctctgtt caagaacctc ctccagtaag
    28381 gaatgaacag cttcatcgtc ttcgataatc aaaatagttt ccagcaggac cacctactta
    28441 atgtcagatt tttggaaaat aaacattcct agagctgatg ataccacgat tgttactaga
    28501 ctgaccagaa tcattttcgg tgcttgcgaa taagttgctt gttgcgcaaa gtccgtatac
    28561 atgagccacg tttccgtaaa ccactccgtt agaaattcca agcctttgat ttttgcgaaa
    28621 acaccgggaa tgaaggccgt tcccatgata taaagaaaag aaagaagcaa actgacactg
    28681 gtacttctag tgatgaatgt caaagccact ggaacagata atgtccctaa tgtcagcaga
    28741 agcaatacag gaaatacttt tagaacataa tttccatagt ttttgattcc ctcaacttca
    28801 ccaaatccga aagcaagtgt gtaagcaagc atggctagtc ccgtgatcaa aaccacgcca
    28861 agtgttgtga tcaaaagcgt aacgagaagt ttcgacacat aataagtcat tcgattccgt
    28921 cctaaagata acgtattgcg aatcgtaccg ttttgaaatt cttccccaat ataaaagcac
    28981 gcaaatgctg cgaaagccag aagaatccat gcagccattt tttctaccat taaaatccct
    29041 gttgcaccgt tgatgactgt atccgttgct tggtttaatt cattgccgta ttttgtatgc
    29101 atataccatt cgttccaaga ataaaaaagc ggaagtacga ccatcagtcc tagtgagacc
    29161 cacattttac ggctcttcca tacttttatt ttttcagcag ttaatagatt aaacatgtgg
    29221 attgcctcca gttaattgta agaaataatc ttccagtttt tgcccaacta ggtgaatacc
    29281 atccacagga acttttgctt ctaccaatgc ttgattgatg gctgctactt ggtcaattcc
    29341 ttcaaaaagc tggataacac ggctagattg tacaaaataa tccctataac caagagagtc
    29401 gagaaccgtt actgcttgtt ccgtcgccga agtgtcaatt tttataaatt gacgagattc
    29461 ttgtgccaat tcttctttcg ataattcttt gatcaagtgt ccttcatgca aaataccata
    29521 gtgagtggcg atctgtgaca attcatctag caagtggcta gaaagcaaga cggtgatgcc
    29581 ccgttctttt accaaacgtt ggatgatttc tcgcatttct gccatccctg ctggatccat
    29641 cccattcgtc ggttcatcca aaatcaaaaa ttctggattc gtgatcagtg catttgcaat
    29701 agctaaacgc tggcgcatac ctaaagagaa attctttgct ttctttttcc ctgtattttc
    29761 caatcgcatc attttcagca ggtcttcgat ttctctatcc gacacaccgc cgttcgctgc
    29821 ttggatacgt aggttatctc ttgcagttaa ttctggataa agagcagggg tttcaatcga
    29881 ttgacccata cgtcgtcttg ctgcctgcaa atccgtcgtt ccaaataatt cgatcgaacc
    29941 ttcatcaatc gagatcaaac ccataatagt tctcataaag gtagacttac ccgctccatt
    30001 ttccccaatc aatccataga tcattccacg tttgatttcg attgatactt tatctaatgc
    30061 tttggcagca ccgtattttt tggttatacc agttgctttc aagaccgttt catttccaaa
    30121 atccatactt atccttcttt cttattgatg agtgaagtat agttgacggg tataaagaaa
    30181 gcagccagaa atgtataaag aaagtgtaaa ttttccaatt gaatgtcaaa aaaaacaatc
    30241 ggacaatacg tgtctttcct attatcaggc agtttgttat acgcttgtat cagtttcctg
    30301 attctttctt ttcgaccaaa gtcgtttctc tgtattcctc ttcactgaaa atttttgctt
    30361 ctttcagatc aagcttgagt aattttacca gaccggttat aaggataact atagcaagac
    30421 tccctcccag aaaaattaga ctgtctcgca gacgtggttg cgttgtcttc caatcaaaga
    30481 gcgcaccaaa cagtgctaat ccaagtggac tggccaccat ggtaatcgtt gttatcgttg
    30541 acataagtcg tccttgtata tgcgttggaa cttctcgctg aagccagacc atataaaaag
    30601 caaaagaaaa cattaatcct taaaaacgga cttatgaaaa ataatacatg ccgtttggga
    30661 tttacaatta gctaccaaat aagaggttat gacaaaagac ttttcacaat cccttcttcc
    30721 gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattt atcaaacatg
    30781 agaagcaatt tttgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct
    30841 cagaagcgga taaataatat tgtttgatga tactttatct ttttcccgtt tgtttataga
    30901 tcgaatgata ttatagacca agaatcctac ctattaacat agctaaaaac acaaaaaagg
    30961 cactttccaa attgtggaaa gtgccagaaa agttaggatt aaggtaactg cgctcagcat
    31021 tgcttcgccc agtacgattc tcgtaactcg tgaactcgaa cgataatcgc aattaacgtt
    31081 ttgagaactg tgaagcttta cgggctttct taagacctgg ttttttcttc gtatcattga
    31141 ataagtttga aaaaccttga tttataaagg ttttcccgta aatatatttt attatatttg
    31201 attaaatttg aaatgtttgt gttctaaaat gtgt
//
LOCUS       DAFCHQ010000025        30465 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000025 DAFCHQ010000000
VERSION     DAFCHQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.025"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(456..1766)
                     /locus_tag="LNC71_001212"
     CDS             complement(456..1766)
                     /locus_tag="LNC71_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK6466294.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1778..3136)
                     /locus_tag="LNC71_001213"
     CDS             complement(1778..3136)
                     /locus_tag="LNC71_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6466295.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3338..4906)
                     /locus_tag="LNC71_001214"
     CDS             complement(3338..4906)
                     /locus_tag="LNC71_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBK6466296.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4910..5320)
                     /locus_tag="LNC71_001215"
     CDS             complement(4910..5320)
                     /locus_tag="LNC71_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBK6466297.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5317..6240)
                     /locus_tag="LNC71_001216"
     CDS             complement(5317..6240)
                     /locus_tag="LNC71_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBK6466298.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6237..7115)
                     /locus_tag="LNC71_001217"
     CDS             complement(6237..7115)
                     /locus_tag="LNC71_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBK6466299.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7102..8439)
                     /locus_tag="LNC71_001218"
     CDS             complement(7102..8439)
                     /locus_tag="LNC71_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBK6466300.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8442..9545)
                     /locus_tag="LNC71_001219"
     CDS             complement(8442..9545)
                     /locus_tag="LNC71_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6466301.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9619..11127)
                     /locus_tag="LNC71_001220"
     CDS             complement(9619..11127)
                     /locus_tag="LNC71_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6466302.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11093..11785)
                     /locus_tag="LNC71_001221"
     CDS             complement(11093..11785)
                     /locus_tag="LNC71_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6466303.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11807..12781)
                     /locus_tag="LNC71_001222"
     CDS             complement(11807..12781)
                     /locus_tag="LNC71_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6466304.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13081..13953
                     /locus_tag="LNC71_001223"
     CDS             13081..13953
                     /locus_tag="LNC71_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6466305.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14411..16609)
                     /locus_tag="LNC71_001224"
     CDS             complement(14411..16609)
                     /locus_tag="LNC71_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6466306.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16841..17833
                     /locus_tag="LNC71_001225"
     CDS             16841..17833
                     /locus_tag="LNC71_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6466307.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17893..19314
                     /locus_tag="LNC71_001226"
     CDS             17893..19314
                     /locus_tag="LNC71_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBK6466308.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19307..20806
                     /locus_tag="LNC71_001227"
     CDS             19307..20806
                     /locus_tag="LNC71_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBK6466309.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20845..22239
                     /gene="uxaC"
                     /locus_tag="LNC71_001228"
     CDS             20845..22239
                     /gene="uxaC"
                     /locus_tag="LNC71_001228"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK6466310.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22398..23081
                     /locus_tag="LNC71_001229"
     CDS             22398..23081
                     /locus_tag="LNC71_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBK6466311.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23083..23919
                     /locus_tag="LNC71_001230"
     CDS             23083..23919
                     /locus_tag="LNC71_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6466312.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23930..25684
                     /locus_tag="LNC71_001231"
     CDS             23930..25684
                     /locus_tag="LNC71_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466313.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25960..26301)
                     /locus_tag="LNC71_001232"
     CDS             complement(25960..26301)
                     /locus_tag="LNC71_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6466314.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26369..28540)
                     /locus_tag="LNC71_001233"
     CDS             complement(26369..28540)
                     /locus_tag="LNC71_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK6466315.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28709..29608
                     /locus_tag="LNC71_001234"
     CDS             28709..29608
                     /locus_tag="LNC71_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBK6466316.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29679..>30465)
                     /locus_tag="LNC71_001235"
     CDS             complement(29679..>30465)
                     /locus_tag="LNC71_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6466317.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9100 a         6274 c         5392 g         9699 t
ORIGIN      
        1 gtttattgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
       61 gttttcaaag gtctacttca tcgagagtgt ttcccgattc atttttggct gccgtagcaa
      121 cttttatatc atatcagaga gacaaacgat tgtcaactct tttttaaaag tttttttcga
      181 tttcgttttt cgaaatcttt cagcaactca tttatcataa cttatcgttt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc gcaagtggtt ctttttaacg
      301 acttagttat cttatcacgt ctaaaaacat gtgtcaactc ttttttggaa ttttcataaa
      361 atgtttcaat gaagcgcgac aacgttgttt aatatatcat taaaaaaaat aaaggtcaag
      421 ctaaaatatt atttaagctt gaccttatta taatttcaac gattgtcaaa agtattgttt
      481 gcagctgtgt tgccagtttc tatagataaa tcacagcttt tgaaacataa gttttgtact
      541 gcttcgattc gcccgaattc ttttgcagtt atttggcaat gagaaaagca gacatcttct
      601 ataggctttt caggaaatgc tactaaatca aaagctcggg aaggaagtga gtaacctgga
      661 gaaaggcatg cctgtacgtt tttgatgtgt atgtctttga ctttcgtcat ttgctttgat
      721 tcaggtattt cttcagccac tgcttgccaa ctttctggca tttgagcaag gtcattgcta
      781 ttttttctat tgtattcatt atgccagtcc attatccaag aaaaaggaaa ttgtacatca
      841 atcatattca gattttctac cctgatattt tcgatcactc cccctctttc ttttgaagat
      901 ttcattcgaa taccacagtc tgactgaaaa aaatcgatat cgtgaatata tacgtcacta
      961 actccagcac taacttcgct accaatagtt acaccgtatc ctgaacggat tttacatcta
     1021 gcaacctcga ttcttgatga ttttcgattg actcttgcac cattgccatc tctgccagac
     1081 ttaatagcaa tacaatcatc accacaggcc aaatcacatt catagacacg cacatttgtt
     1141 gaagaatcaa tatcaatccc atctgtactt ggtccatcat tgttgcggat gattactttt
     1201 tctactaata catcgttaga ataagtgatt tgcaagttcc aaaaacctga tttctgaaaa
     1261 atcaaatcac gaacaacaac atgttctgca tgatacagta aacaagcgcg tggacgttta
     1321 atagcataat ccgcaatcca acgaagtccc ttttgatcgt attcagctct tgtcccgctt
     1381 ttttgatctt ttccccaata cagctcccac caatggtctc cttgaccgtc gattattcct
     1441 tttccttcta taagaatatc tttcgccgag agaacattca aaatcgctgc tggccatttc
     1501 atttctacgc cggctactcg tgtatcgatt tctggaaatg cttcgatatc tgttctcccc
     1561 ttgaggactg caccttcttc aaagataaga tgactcttat cttttaaaaa aagggctccg
     1621 actaaataaa tgccttttgg aataaataca ggtaagtgtt ctttcgcacc ttgatcgatt
     1681 gctttttgca gtattattgt atcatcttgt tgagtgtcgt ttgttttaaa gttcaaagca
     1741 ttgataatcc ctttttttgt ccgcaatacg tttctcccta tctttcattg atttttttca
     1801 attcttcacc aataaataca gtttttgaac gattcagtgg gtaacctact agcaaaatga
     1861 ttgcggatac aaataagcag aatgctggta acaagtttgc catcgtataa atcctttctt
     1921 gtactaaaac tgattggatg gctccacctg ttgtagacga ttggtaacca atgagtgcca
     1981 gcataaatcc gcctaccgct cctgcacatg cttgtcctac ttttctcgca aaagaattta
     2041 ctccatatac tgttccatct tctcttaagc ctgtcttata ttgatgataa tcaatcacgt
     2101 cagtaatgaa tgcccagacc atcaaattga ataaaccaga tcctaaagta gcgataaata
     2161 aaagaatcag atatacccat gcatttgtta tatgaaggaa atacatcagc aagtacatac
     2221 atgaagaagc aagtaacgca aatacgcttg cttccttttt accaaataat tttgtcagct
     2281 ttgtagcaaa tggtgctaaa actactactg ttccgtaagt aaataaaaga gcaacagaca
     2341 ttgcttgttt attatggaag taatcattga ataaataagt catattcgtc ccagctaaaa
     2401 tctgattgat cacaatgaaa atgtcaacaa tgactaaaat aatcagtgct cgattagcta
     2461 acaaaccttt taccaactga gaagcagata ctttttcgtt tttgacaact cttactcttt
     2521 cagtagtcaa tttacaagtt aacatatatg cgacaaatgc tattgcagca catccgattg
     2581 aaatccaaaa gaaatgattc cctgaaacga cttgttgtcc actcttgtta tgggtataca
     2641 ttaaaatcgg aatgaagaat cctgtagttg atccgccaat cgcagaacca atgcttcgga
     2701 acgtggacaa cgaggtgcgg tcattaggat ttgagctaat tgctgctgcc attgctccat
     2761 acgggatatt gattgttgag agaaaaatcc cataaacaat gtatgaaaca aaaatataag
     2821 caatcttcac cgtcattgga agatgttgga cgaatggcaa aaacaaaatg acagtcacta
     2881 agcaaaatgg atacttcatt tttctgatcc aaggaataaa tcggccagtt tctgttaact
     2941 ctgagacatc acataatctt ccaaccatca catcagcaaa cgcatccaaa attctcgaaa
     3001 tcaagaataa cgtacctacg attgcccctg aaacgccaag aacattcgta taataaatca
     3061 tcaagaaact attgactaaa cctaaaataa aacagttacc tagatcaccg aacatgtagc
     3121 ctatcttatc ggaaatccca aattcctttt tttcttgttt ttatattcac aatgttggct
     3181 ttttattgca ttatcttgac agataaaaaa caaaaaataa tctaaaaaca ttgatgcata
     3241 agattgataa ctttttttac ataacaatat atacaaacaa aaaaattcac taactagtga
     3301 aaataagtta gtgaacctca tttattaaag aattaaatta cagatgatct tttccatcat
     3361 acttgaaaaa ctcaaaatca gcataacttc ctcttttttt atttagatca tgcactgcta
     3421 tcccgataaa atttccagta aaccctcctg agagtataca catgtttttt tgcggaccta
     3481 ctttcttcca ttcttcagtt gatacgtctt tcaaatgaaa ctgtgctgtc tctttcttta
     3541 cagtgacttt caaaaaagtc ttcttttctg tgagtggaat cgtccaatct tctatttgtg
     3601 ctactccgtt ttctgattct atcatacgta ggcattttcc cttgtcctca ttccaagtaa
     3661 cataacaata aatataattg gattcattta agtacagtac cagtccagcc atctgattaa
     3721 aattattagg catgtattca aatgctgtct ccgcatcaaa agtaaaatcc gtttgccgaa
     3781 tagctaacaa atgatgttcg aaggtagatt gaagagattc accggaaatc agacgtaaaa
     3841 aacctggtct acattttgta tcacaccaat cagcatttgg caaaatgcgt ctggcattcc
     3901 agttcttctt tagctctcct tcaaatgtat ccataaactg atgatctttt tcttgttgta
     3961 cctcttcatt tgtctcaatc attatttctt ccatcggagt tgtctggcta tctttaagtc
     4021 ttaaccaatc gtcggacgtc caatacactt cttgaatagc ggtttctctt cccaaaatag
     4081 ccgcaccgtt taatggtctg gtacaaagat atgtcatgta ccatcttcct gacggggttt
     4141 gtactaagct cccatgtcct gtacattgca atgtagaagc aggtttgtca ctagctgtca
     4201 gcataggaaa accaggatca agctcgtaag gaccagtaat attctttgct cggcaaactg
     4261 taactgaatg tcctcttcct gtaccgcctt ctgccgtcat caaataataa tacccgttac
     4321 gaaaatacaa atgtggtgcc tccgttttcg ctaattctgt acctgcaaat attttataga
     4381 tgggaccatc aagcttcttc ttctctgcat catattcttg taatacgata ccgctggatt
     4441 tattcggtgt gtccagacga taatcccata gttcattgac aagccatttt tttcccgttt
     4501 caagatcatg gaacaaagaa ggatcaaagc cgttactatt taaataaatt ggttcggacc
     4561 aaggacctgt gatctcagat gcagtgatca aataattgtg gacatcttta aacggacggc
     4621 ctgcactttt gacatctgtg taaacaagat aaaacaatcc atcagcataa ctgatctgtg
     4681 gcgcccatat acttccattt gtaggatttc cttgcaaaga tacttgagtg gtcaaaatat
     4741 ccgttgtatg tgaccagttg actaaatttt tcgactcata tactcggatt cctggaagcc
     4801 attcaaaaga ggagacaata atgtaatacc gctctttcac tcttaatata ttaggatctg
     4861 gattgaatcc tcttaaaata ggatttttca ctttgatagt ctgcatgatt caggctcctt
     4921 ttctcattct tactcgatat aaactgaatg ccccagctgc gaaataaaaa aatgcaaaag
     4981 gaaacgcaat aaactgagtg ccaatcaaca atatgaatcc tgttataaaa tacaaaccag
     5041 tggcagttgt taagtatttt cctttagcag tcaaaaacaa tgctactgct aaaaaagcaa
     5101 gtgaaataaa caacacaacg gcaaacagac cagtcatgtc ctgtaataat ccaaatgtat
     5161 tttctaaaga caaagttgaa tgatctacag caagcgactg ataacttttt ttaaactctg
     5221 acgcagacat attacttaaa gtaactgcta acccgccaaa gaaaatcaaa gatagactgc
     5281 caccgaccat tcctagcaat agttcccatt ttcttttcat ttctgaaccc cctgcttttc
     5341 taaatgtatc cattcaggtc ttttgatcga tgtttcataa ttgttattac gttcagagaa
     5401 tttgacagtc tgttcatttt tcttgtcatc ccaattatcc caaccagttg ggtgaacatg
     5461 cccacctatc ttacacgctt caaataagac atttgcatac ggacgccacg gtcttcctaa
     5521 atagacgttt ctagtatcag gttctgcagt caaggtacag ctttcaaaac gataccctgt
     5581 tttttgattt ttcggcgtag atgctgctgt gatgtatcca ggatttttac tggctaagct
     5641 atggatatga cactgatgaa aaacggcatc tgccccacca aatatgaaat ccaccgttcc
     5701 ttcaatccag caatattcat aggtctgacg acaatactca ggattggcag aagccggtgt
     5761 acaaaaagga gtaccatctt tttgcaaatc aggcaacgga cccgtgcata aggtatcctg
     5821 ataaccagat agtcgacagt tttttaaaga tacgcgatct cctgaattga ataaagcaac
     5881 agcctgtcct accttttctc ctggacctgc tgtattttcg atgtgaagat tttctaatac
     5941 gacattcgat ccttctaaga aaaaagtagc acttcggaaa gtccctaact ggctaccgtc
     6001 aagatactcc tgttttgcaa aacgattacc ggagataact actgttcctg ctcccacaag
     6061 tttaaaattg gataatctgc tttctatata ttcatcatat cttccttctt ttactatcaa
     6121 gacctttgga tggctatccg gcaggcctgc caaataatcg attccttctt ggatagtttc
     6181 aaaatccgtc ggggtatcta tagcattctt tccaattatc agtttgtctt tcatcctcat
     6241 aaatgctccc ttatgaattg tttccaaata tttctcattt cttgtgtttc catatgaccg
     6301 cctgttaaag aaaaagaaga aaagttttct ggactaccca ttgattgata ctttttgtcc
     6361 agcccttttc tcagcttcag tacgccttcg tccaagcaca tcgtatcgtt ttttccaact
     6421 aagctaagac gagggcgcgg agcgattttt tcttggattg ctaacgttgt ataagctgtc
     6481 aatagatttg gcacatagta ataaaaccca tgatgatcta ggcggcgatg tagcaaaagt
     6541 atttcgatgt ccacctgtgc cgctagatca atacaaactt tgacacgaga atccaatgct
     6601 gtcacccacc agcttaataa gcctcccata gaaagaccaa tcgttgcgag ccgttgagta
     6661 tctgtgtctt cccttgtctc caaataatcg atcaagctga tcacatcata aatacgcatt
     6721 ccccataacg tttttcctgt taataaaaat tgtttgaaca actcgctttc agaaatacct
     6781 ccgcgttctt caaatcccca agcatcaatc gaccaaacag catatcccat tcctgtcagt
     6841 acttctgcaa aagatggaga agctaagtaa ggagcaccat ggatcaattc acttttcccg
     6901 actgaaaaat ctccgccgtg agaatgcagg taaaggaccg gcggaaacgg tccttctttt
     6961 tctggataag catagagggc tggtactgtt tccaattcat ttaagtacaa ttgatagtat
     7021 tcaagcataa aaccgttttg tttttctcgg tataatcgtt ttccactagg ggtatgtctg
     7081 ctagatggag cacctaaaaa atcattcaac tgcataagca aagtttttac gaacaaccag
     7141 ttcatccata ttttctgatt gatttgattg gaatttctca atactgattt ctttagtatt
     7201 ttctaataga aatgctggtc cctcgtgatt ttcaatcgac atctggttaa aggaaacctt
     7261 acccgcaaag ccaatataaa atccttgtct gctcatttcc tctattcctg tcatcatagc
     7321 aggaacaccg ggaactgcat cttgggccat cgagacatcg atattgtcaa atgtgatttc
     7381 ttgtatgaat tgttccgcta atccataaat aaaacctgca gaagcatgga cattccttgc
     7441 cgtgatattc gaaaaatgga tccttctgaa tgctggtgtc cgttcatcta tcggataagc
     7501 ctttttttcc catacatacg gttctttccc tcgcggacca cagaaataat aaagattcag
     7561 tataaatgga cacatcacat tatccatgac tatattggat actcgaatat cttcaacgat
     7621 cccgcctcga cctcgtctag atttcaatcg tattcctcga tcagtttctt ggaagataca
     7681 atttgaaatg gtaatattac gaatactacc actcatttca cttcctaata cgacaccgcc
     7741 atgtccatgg accatcgtgc aatttgtgat cgtgatattt tcgcaagcga tcctttcgta
     7801 cgtatcttca gtccctgcct tgattgcaat acagtcatct cccacgtcaa tatggcaatt
     7861 actaatacgc acatttttgc atgattctgg atcaattccg tctgtatttg gtgaatccgc
     7921 tgggttcaag atcgtcaagt tatcaaacgt tgcattactg caaagaatcg ggttgatcgt
     7981 ccaacttggt gactggatca atttaatatc tttcacagta attcgatgac agttatggaa
     8041 actcatcaat ttaggacgag gataagcgag attatctggt tcatttctaa aagtatgcca
     8101 ccattttttt ccatttccat ctaatgtacc gaaaccagtg acagagatgt tctctacatt
     8161 ttgagcatag atacaggaag cataaacttt tcgatggact ccttcccaac gtgaatgaac
     8221 aacaggataa tcttttggat cgtcagagaa tttcagaacc gctcctgcgc ttaaatgaag
     8281 ttcgacattg ctttttaaaa ataaagctcc tgttagaaat tcacctgctg gaacaactac
     8341 ggtcccgcct ccgtcacttg ctgcagcatc aatggcttgt tgtattgctt cagtattcaa
     8401 ttcatcaatt gatgcgccaa atgtcaaaat atcatacata tttataatgc ctccacttca
     8461 atcaatgctt gcaagaatgc accaacgcct ttttgatcat tacaaataat tggttcactg
     8521 atgtagtaag catatgaacc atctcgctga tctgctccgc ctaaccctgc tacttgacag
     8581 tttttattca agttcagcca gccttctttt gtttccagaa caaattcatc aagtaatcct
     8641 tgatgtgatt tcttcacttg tggaagccat tcttctttgt tcagcagtcc caaacgtatt
     8701 cctttagcca ttgcgtaaac aaacatacta ctgccggaag cttccagata atttcctttc
     8761 cgttttgttt ctgttgttac ttgataccat gtcccgctgt tttcttcttg atatggaagt
     8821 aatgcagcca gtgtcttttc tagaatttct ttcagcaaag aacaatctgt ttcttcatga
     8881 agtaattcat aggtatctgc tgcagccatc aaataccagc caatcgatcg gctccaaaaa
     8941 tgatgggata gacctgtttt tggatcagcc catggttgga ccttcttttc atcccatgca
     9001 tgaatcagca aatttgtttt tggatcgaat agatgtttat aactgatttc aaactgtctc
     9061 aaaacatctt ggtaatcttt ttcttcacta aaattctgga taaattcagc ataaaaagga
     9121 gaacccatat ataagccatc caaccaaatt tgataaggat aaatttcttt atgccaaaaa
     9181 gctccttcgg atgtgcgtgg atggtttgcc aactgctggg aaaggagttc tgctgctttt
     9241 ttatattttt cttcgcctgt ttctttgtac aataaaaaga aaagctttcc tgtatttaca
     9301 tggtcgatat tgtattcttc aacgctgtag ccacggatcg ttccatcttc ttgcacgaaa
     9361 tagtcgatat tgtcttttat gaaatcaaaa taaatttttt cttgggttcg ttcccaaagt
     9421 aattggaacc ctttaagtac gacgccatag tcataagacc attttccttt ataccacttt
     9481 tcttcatata agcgaggtgt tctttccatg atactttttg ctaatttctt tgaccagttt
     9541 tccattattt gctctccttt aaaaattaaa ataggatttg ggacaaatac caagaagtat
     9601 ttgtcccaat cgtctatatt actgtgccgc tgctttgtat gcttcctcgt actcttgtgt
     9661 gattcgatcg cctccttgtg ctttccagtc ttctacagct tttttgaatc catcttcgtc
     9721 aagctgtccc atgatgtatt gcaccgtagc atcaatgacg attttttcaa tttctgatcc
     9781 tacttcattg ttcgtcggtg aatccaatgg cacagaagga tccattacag caaattcttc
     9841 attttctttg atcaattgat tagagaattc tttttcaggg tctgtatctt tgaaagagaa
     9901 cgtcacttca ctaggacgag agcttgataa cggttgcacc tcttgttgcc aaagatccat
     9961 atcgataaat tcgatagaac catcatcatt caattcataa tgcgttcctt cgataccgcc
    10021 agtcatcaat ttatacacat cttcttctaa aagatcatca acaaattgaa gtaatttctt
    10081 caattgtgct tcgtctttta cttgagattt gggaaatgct agcaagccgc caataccatt
    10141 tccttctgac caaacatgat aaccgccgcc ttcaccatct gagatcttgt ttacaggcac
    10201 taattccata tcatcttgaa taccttgacc catcgtcttc agattcttga tatccaccat
    10261 tccagtgtag attccagttc ttccttgtgc aaattgttct tgctgatcag tcttttgggt
    10321 aactgcaaag tcttgtgcca aatagccatt ctcaaataga tctcttgaaa aattcaaagc
    10381 tttcatatac tcttctgtat caaattctgg agtgaatttg ccattatcgt ctacagccca
    10441 gccatttggt gctccaaaat aagaagtgaa aagtttaaag ctggtataac gaatatcact
    10501 aggcgcacga tcgccaaaac caactgtgtc gtccacacca tttccatcag gatcgttttc
    10561 tgtaaattgt ttagctactt cataaagctc atccatggta gttggaacat ctagtcctag
    10621 attatccaac cagtcttttc gaatgactaa gccagcacga gcgtagtctt tttgtatcgg
    10681 tacgccgtac aaggcaccgt cgatactcgc agcttcgatt ctatcttcag aaatttgact
    10741 taaattttta tagtctttta aatattctga tacatcccag aatacgcctg attttaacgc
    10801 attgcggaca gaagagtttt tcaacatcgc aatttgaagt gtaacgatat ctcctagttc
    10861 atcagaagcc aatgcagttg ttaatctttc ttcttttgaa gcatcaggaa tccagttgaa
    10921 tgtcagttcc gtatcggttt tttcttcgat caagttcaca atcgtgtctg taggcggtga
    10981 agcatgatgc aaaatattta accaagtgat tttagctttt ccatcttctg tctctgaaga
    11041 actgttactt gaactgctgc cgcatgcggc aagtgctaga attcctgcaa aactaagacc
    11101 taattttacg agcgttgctt ttttcataaa ttttctcctt cactgtttta ataatatttt
    11161 tgttttcttc tgttttttct gtatatgagt tcttccattc caaatggcaa aatatagaat
    11221 ttgctgtcca cgtgactgcg gaaaagaaga gactgccacc gataaaggtt aaaacacctg
    11281 gcattgtttg gatgatcatc agataaatcc ctaccatact taatatcaac actaatgagt
    11341 aatgaagatg tgagaatccg aataaaaatg ccattttcat tttgaaaaaa tatcctttcc
    11401 aatcataatg agccatgatt gggaaaatat agagtgctga gagcacatat atgaacgcag
    11461 caatatacaa agcgatccta agataccaat tgtgtatgaa agaaagatcc acaagtaaaa
    11521 caatcccgat ggcgccatat atccagctca tcagcaaggt ttcacgatag ttttctttaa
    11581 aatatgtgaa gtacgttttc gtcaaaggaa acgtttcttt tgagcgcagc cattttctaa
    11641 tcactgcaac catcgcatac gatgcaggac cgaaagtaaa aactcccaaa cctgcgatga
    11701 ctaaaccgcc ccacagaaaa ttcaaataaa ccaactgtaa aatacgattt agccttccgt
    11761 ttattttatc taatgactgc agcatatctt tccctctctt tctatcttag taaacgccgt
    11821 cttctcctag ctttttggca atcttattag ctaaaattac cataaacaat cctacgaccc
    11881 ctttaaacaa tcccattgct gtactgaaac ttaactgtcc attcttcaat ccagctgtat
    11941 aaatataggt gtcgaaaatt tctgcaacgc ttcgattcaa tgaattcaat aacaagtaca
    12001 tatgctcgaa ccctaagtct aatgtctgac cgatttttag aatcagcaag gtaatgatta
    12061 ctggacgaat tgccggcaat gtaacatgcc atgttttgcg taagcgtcct gctccatcca
    12121 tttctgcagc ttcatacaac tgtgtatcga ctgcagttat cgctgaaagg tagatgatcg
    12181 tagaccagcc caactctttc cagatcactt gtccgatata tactgtgcgt gtccattcag
    12241 gggatgttag aaaactgatt ttttcaaatc ccattgttgc aaggaattca ttcaataccc
    12301 ctccgtcaac attcaaaaat acataagtaa tcgaaacaat gattacccaa gacatgaagt
    12361 gagggatgta aatgatcgtt tgaatcccgt ttttcaatcg tttgttcgtt acttcattta
    12421 atagtaaaga caaaatgatt ggcatcggaa aaaacacgaa gatatttaat ccgaacaaga
    12481 tcaacgtatt tcttaataat gtaaagaatg tcggttctgt aaacaaacga atgaaatgtt
    12541 tgaaaccgac ccaaggactt cccattattc ctaaaaatgg ttgataatct tgaaaagcaa
    12601 taacgagtcc ccccatagga agatacctaa aaatcaagaa ataaaggact cccggcagaa
    12661 tcatcacata taaaaattta ttcgttttga ttcctgcaat tatttttttt cgtcttctga
    12721 gcttttcttc ttttttcgat agcgtcagtt gaaatggttc ttgtttcttt acactttcca
    12781 taagcccctc cttagttttg tgaatgtttg ctgcttatga cttcatgata gataactatc
    12841 tgaattacgt atataatcat ttttaccgta agtaaaaacg cttacatagc aatgattttt
    12901 gtgctataca aaacgataaa gtgtattttt tcacaaataa actacgcata gcaaaccttc
    12961 tatttttttc tatctgacac ttagcatagg aaatgattat atacgtaatc tttcaccgat
    13021 gataaattga acatatagaa acagaaacat ttaatttatt ttgaggagga gcctatctat
    13081 atgaaaaaga aagtctctat aggagaaaga agttttacac ttttcaacaa tacgtttcta
    13141 attttgctag ctttgatctg tatcgttcct tttttaaata tcatcgctac atcttttgct
    13201 tctacacaag aagtcgtcgc gaaaaagttt atcctttttc ctactacctt ttctttagac
    13261 gcgtatcgct acattctttc tacgccgacc atttttagag cacttgctgt ttcgattggt
    13321 gttacaggtg tcggaacgat cgtcagtatg tgtgcaactt cactgatggc ctacggtctt
    13381 tccagaaagt atttgtttgg tcgaggtttc gtcaactttc ttgttgtatt ttctatgttg
    13441 ttcagcgggg ggatgattcc aacgttctta gttgttcgtt ctctagggct ggtcaattct
    13501 tactgggcca tgatcctgcc tgtggctgtt aatgcaatga atatgatcat tatgagaaac
    13561 tttttccaag ccttgccgga tagtttggaa gaatcagcaa aaatggatgg ttgcacagat
    13621 tttggtgtat ttttcaaaat tatgctgcca ttagcgttac cgtctattgc tactatctcg
    13681 cttttttatg ctgttactta ctggaacact tatatgactg ctattttgta tatcaacgat
    13741 tcatctaaat ggccgataca gattctttta cgccaaatcg tcattgtatc aagtgggatg
    13801 caagcagaaa gtagtgccgt tgatgtgatt ccaccagcac aaacagttaa aatggctgtt
    13861 atcgttattg caacagtgcc tatgctgatt gtttatcctt ttgttcagaa atattttgtc
    13921 aaaggagcac tcgtaggttc tgtcaaaggg tgattatgta ctgaacgcaa aatggtagtt
    13981 gtgtcagaag tggtcagctt caagaaataa gaaggcattt acgaaaattg cttcttaaat
    14041 ttttgtgaaa tttcggctta tttttcgaag aatctactcc tggaacaccg tgtatcagag
    14101 gttgtgaaag agacgttcag ccttgagcga tgataaaaac tctcacaaac attgcgacga
    14161 gtaacccaca ggagcaataa gaagcgattt tgaaaaatag cttcccatat ttcacaaaat
    14221 tatgccttat tgtcgatagc ctgtctcttg aacgccattt attcgtaaaa aagaactgtg
    14281 acaagacttt tgtcacagtt ctttttgcgt gcttagcctt gaaatttaac tatccataaa
    14341 tacaaatact agggttattc taacttcttt tattgcttga atcagtccac aagaaaaaca
    14401 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
    14461 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
    14521 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
    14581 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
    14641 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
    14701 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
    14761 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
    14821 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
    14881 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
    14941 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
    15001 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
    15061 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
    15121 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
    15181 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
    15241 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
    15301 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
    15361 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
    15421 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
    15481 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
    15541 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
    15601 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
    15661 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
    15721 gggcaaatct gtccgaaaat aagtaggaaa gaagaccaat gagtcctacc agtgttaaag
    15781 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
    15841 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
    15901 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
    15961 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
    16021 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
    16081 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
    16141 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
    16201 caatttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
    16261 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
    16321 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
    16381 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
    16441 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
    16501 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
    16561 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
    16621 cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat ctaattaaat
    16681 gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt aatttttaac
    16741 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
    16801 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatgt
    16861 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
    16921 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
    16981 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
    17041 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
    17101 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
    17161 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
    17221 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
    17281 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
    17341 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
    17401 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
    17461 caatccagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
    17521 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
    17581 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
    17641 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
    17701 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
    17761 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
    17821 caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc ataacacaga
    17881 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
    17941 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
    18001 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
    18061 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
    18121 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
    18181 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
    18241 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
    18301 cccccacaga gcctcccggg caaactgaca gcctttttat accgacgttt caaattaaac
    18361 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
    18421 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
    18481 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
    18541 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
    18601 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
    18661 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
    18721 gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt ggcggaattg
    18781 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
    18841 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
    18901 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
    18961 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
    19021 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
    19081 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
    19141 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
    19201 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
    19261 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
    19321 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
    19381 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
    19441 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
    19501 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
    19561 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
    19621 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
    19681 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
    19741 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
    19801 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
    19861 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
    19921 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
    19981 atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
    20041 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
    20101 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
    20161 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
    20221 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
    20281 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
    20341 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
    20401 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
    20461 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
    20521 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
    20581 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
    20641 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
    20701 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
    20761 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
    20821 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
    20881 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
    20941 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
    21001 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
    21061 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
    21121 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
    21181 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
    21241 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
    21301 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
    21361 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
    21421 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
    21481 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
    21541 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
    21601 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatcgt
    21661 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
    21721 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
    21781 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaaccgt
    21841 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
    21901 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
    21961 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
    22021 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
    22081 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
    22141 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
    22201 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
    22261 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
    22321 cggaccaaat ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt
    22381 atgaaagaag agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa
    22441 aagatgaaaa caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac
    22501 ccgagatcca gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg
    22561 aaggaaggtt agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat
    22621 ttggacataa cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt
    22681 taaaaaatct gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac
    22741 tgacatctgt gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg
    22801 gagactatcc aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg
    22861 tgttctcaag atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct
    22921 acctgcatct tatacctgat acttgtaaaa attacccaga aggactaaaa gacaataccc
    22981 actttagtcc agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat
    23041 cccgtctgcc tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta
    23101 ggcatccgtg cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca
    23161 cagcttcact tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa
    23221 ttaactgaca tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat
    23281 atcgaacttt ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt
    23341 gcacaagcat tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag
    23401 atgattgcta cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa
    23461 gaagcttatc agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt
    23521 ggcgtaactg ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct
    23581 aaacgtttaa tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat
    23641 ttgatgcata aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat
    23701 ttacatggtt atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc
    23761 cgcatggtac ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa
    23821 tatgagcgtc ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa
    23881 gcaatagaac acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg
    23941 aatacagaaa agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta
    24001 tcagggaaat tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc
    24061 atcttggtta atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc
    24121 ttctcagttg caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa
    24181 tatattgacg aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc
    24241 tggactaaga gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga
    24301 attgcaatct ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt
    24361 gtcctctcaa gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg
    24421 tatcacgctt tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc
    24481 cgcattcgtt actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca
    24541 agaaatggaa gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg
    24601 agctcatggg ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta
    24661 tgaaaaagga ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag
    24721 ttttcagata tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg
    24781 catccaaccc ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt
    24841 ttctggaatg atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat
    24901 tggcgaactg ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca
    24961 tttggatgga aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc
    25021 gattatcgat catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc
    25081 atctggctct ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc
    25141 aggaaaaatt tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa
    25201 ttatttagcc gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat
    25261 cacttatggc ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca
    25321 actttcttca ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg
    25381 caacaagctg tctggtggcc aaaagcaacg tatctccatt gcacgagcgt tgatacgtca
    25441 gccaaaaatc attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa
    25501 aatccagcaa gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg
    25561 gctttcaacg atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga
    25621 aataggtact tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga
    25681 atgacaaaaa ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg
    25741 gtgttctcct gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt
    25801 tttttgaaat tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa
    25861 tggtgttcaa gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga
    25921 acgtctcttt cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg
    25981 aactggtgtc atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt
    26041 ttgcatcaag tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt
    26101 cattgcatct tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg
    26161 catcgcttgg aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta
    26221 cgcttctttc aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc
    26281 gctatcgtag atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt
    26341 aacatggggt tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt
    26401 tagccgcttc accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc
    26461 cttcttttgc ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat
    26521 tagaatttcc tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg
    26581 catctgctac actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt
    26641 tgaagacttt cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt
    26701 cttcgtaacc catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt
    26761 catttgcttt tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat
    26821 aacctgcagg attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg
    26881 tcatttcatc ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga
    26941 cgatcaccgc tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac
    27001 catcattggc aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca
    27061 gtccaccaag ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt
    27121 ctgctttatc atatcctttt tgtaaaccga tctcatgcaa aagccataca gtaggcaaat
    27181 tcaaactttg tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat
    27241 tatgcgcatc gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag
    27301 cttccagtgc tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca
    27361 tctgtgtagc aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt
    27421 gtacaccacc tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg
    27481 aagcatttgc tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat
    27541 cctgatctaa agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac
    27601 catactcgtt aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag
    27661 aatctgaatt atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt
    27721 gttcttgttc ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc
    27781 gatcattcgc attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc
    27841 ctgccaaagt agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc
    27901 tggaagcgtc ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg
    27961 tcaagatttc ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg
    28021 cttttcgatc aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga
    28081 tcgtacttcc tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg
    28141 caatcccttt gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta
    28201 ctgcatcttg gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt
    28261 acagtgtgcc agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg
    28321 attttaacgt ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta
    28381 agacacagat caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc
    28441 gctttcgcca tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca
    28501 aagcagcttt tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat
    28561 ttgattcaat cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta
    28621 gttcaatatg tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg
    28681 taagtttttt cttaaatatg ttttaaaaat ggtacaatta gtctgcgatt gcactgaaag
    28741 gagtacactt gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg
    28801 cgacttgctt gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg
    28861 taaaaacgtt tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga
    28921 ccagatcaca ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga
    28981 tgcgaagcac atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc
    29041 aggaatgaag actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc
    29101 tgcttatctc gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac
    29161 aagcggtgcc gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt
    29221 ggaacaaaaa atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga
    29281 tatgaccatc accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga
    29341 tgataaaaat ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca
    29401 aacacaagtc tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc
    29461 tcataaggga catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact
    29521 gatgctccat gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg
    29581 taaagcttta cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat
    29641 aatgataaga ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct
    29701 gctaacagca aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat
    29761 tcacttactt gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt
    29821 ttttgaagga gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct
    29881 aacgtcaacg tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca
    29941 ggatgatctt gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct
    30001 gctgctaatc cttctaaaca atctttatga taaggacaag taccttcata atggtcatct
    30061 ggatggcgtt tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct
    30121 tcattgatca cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt
    30181 tttccttgtg ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc
    30241 caagcagcag gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac
    30301 ccttttttgg gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc
    30361 gggccgaatg agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc
    30421 tgatccaatg tttcttctgg tgttgtggta gggatactta atttt
//
LOCUS       DAFCHQ010000026        29428 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000026 DAFCHQ010000000
VERSION     DAFCHQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.026"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1368
                     /locus_tag="LNC71_001236"
     CDS             <1..1368
                     /locus_tag="LNC71_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6466318.1"
                     /translation="DLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYP
                     DGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTD
                     VTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDL
                     NKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTV
                     TDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKK
                     GTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPD
                     GSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGS
                     KGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNR
                     KKENE"
     gene            1502..2845
                     /locus_tag="LNC71_001237"
     CDS             1502..2845
                     /locus_tag="LNC71_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6466319.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2957..4408
                     /locus_tag="LNC71_001238"
     CDS             2957..4408
                     /locus_tag="LNC71_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBK6466320.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4757..5389
                     /locus_tag="LNC71_001239"
     CDS             4757..5389
                     /locus_tag="LNC71_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBK6466321.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            5977..7416
                     /locus_tag="LNC71_001240"
     CDS             5977..7416
                     /locus_tag="LNC71_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6466322.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7711..7929
                     /locus_tag="LNC71_001241"
                     /pseudo
     CDS             7711..7929
                     /locus_tag="LNC71_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8178..8393
                     /locus_tag="LNC71_001242"
     CDS             8178..8393
                     /locus_tag="LNC71_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466323.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8610..8866)
                     /locus_tag="LNC71_001243"
                     /pseudo
     CDS             complement(8610..8866)
                     /locus_tag="LNC71_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9053..9964)
                     /locus_tag="LNC71_001244"
     CDS             complement(9053..9964)
                     /locus_tag="LNC71_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6466324.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(9983..10987)
                     /locus_tag="LNC71_001245"
     CDS             complement(9983..10987)
                     /locus_tag="LNC71_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBK6466325.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(10984..13107)
                     /locus_tag="LNC71_001246"
     CDS             complement(10984..13107)
                     /locus_tag="LNC71_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6466326.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13112..15559)
                     /locus_tag="LNC71_001247"
     CDS             complement(13112..15559)
                     /locus_tag="LNC71_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6466327.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15546..15935)
                     /locus_tag="LNC71_001248"
     CDS             complement(15546..15935)
                     /locus_tag="LNC71_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6466328.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(15995..16498)
                     /locus_tag="LNC71_001249"
     CDS             complement(15995..16498)
                     /locus_tag="LNC71_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6466329.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16511..16735)
                     /locus_tag="LNC71_001250"
     CDS             complement(16511..16735)
                     /locus_tag="LNC71_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466330.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16839..18749)
                     /gene="ltrA"
                     /locus_tag="LNC71_001251"
     CDS             complement(16839..18749)
                     /gene="ltrA"
                     /locus_tag="LNC71_001251"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK6466331.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(19495..19632)
                     /locus_tag="LNC71_001252"
     CDS             complement(19495..19632)
                     /locus_tag="LNC71_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBK6466332.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(19629..20813)
                     /locus_tag="LNC71_001253"
     CDS             complement(19629..20813)
                     /locus_tag="LNC71_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6466333.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(21072..21362)
                     /locus_tag="LNC71_001254"
     CDS             complement(21072..21362)
                     /locus_tag="LNC71_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466334.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(21352..22494)
                     /gene="dcm"
                     /locus_tag="LNC71_001255"
                     /pseudo
     CDS             complement(21352..22494)
                     /gene="dcm"
                     /locus_tag="LNC71_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(22570..22734)
                     /locus_tag="LNC71_001256"
     CDS             complement(22570..22734)
                     /locus_tag="LNC71_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466335.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(22727..23401)
                     /locus_tag="LNC71_001257"
     CDS             complement(22727..23401)
                     /locus_tag="LNC71_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBK6466336.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(23479..24819)
                     /locus_tag="LNC71_001258"
     CDS             complement(23479..24819)
                     /locus_tag="LNC71_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK6466337.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(24902..25237)
                     /locus_tag="LNC71_001259"
     CDS             complement(24902..25237)
                     /locus_tag="LNC71_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBK6466338.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(25247..25621)
                     /locus_tag="LNC71_001260"
     CDS             complement(25247..25621)
                     /locus_tag="LNC71_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6466339.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(25634..25948)
                     /locus_tag="LNC71_001261"
     CDS             complement(25634..25948)
                     /locus_tag="LNC71_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6466340.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(26061..29288)
                     /gene="fss3"
                     /locus_tag="LNC71_001262"
     CDS             complement(26061..29288)
                     /gene="fss3"
                     /locus_tag="LNC71_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBK6466341.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         8826 a         5925 c         5186 g         9491 t
ORIGIN      
        1 gatctaacag ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat
       61 gttactcccg gcggtacaat tgataccaac acaccaggta attacgaagg tgtcattgaa
      121 gtaacgtatc cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat
      181 cgttctgacg ccgataaata tgaaccaaca gtagaaggcg aaaaagtaga aattggaggc
      241 acagtagatt taacagataa tgttactaac ttgccagcgt taccagaagg aacaacagta
      301 acagatgtta ctcccggcgg tacaattgat accaacacac caggcaatta tgaaggtgtc
      361 attgaagtaa cgtatccaga cggtacgaaa gatacggtga aagttccagt agaagtgaca
      421 gacaatcgtt ctaatgcaga taaatacaat cctgaaggac aaaaagtgac tacagactta
      481 aataaagagc cagatgcatc tgagggaatt aagaacaaag aagatttacc aaaaggaact
      541 acttatgctt ggaaagagaa agtagatgtt agcacagctg gaaataaaaa aggtactatt
      601 gtagtgacat atccagatgg atcatctgat gaagttgaag tagacgttac agtgacagat
      661 actcgttccg atgcagataa atataatcct gaaggacaaa aagtgactac agacttaaat
      721 aaagagccag atgcatctga gggaattaag aacaaagaag atctaccaaa aggaactact
      781 tatacttgga aagaaaaagt agatgttagt acagctggaa acaaaaaagg tactgtagta
      841 gtaacatatc cagatggatc taaagaagaa gtagaagtta ctatttctgt agtagataaa
      901 aaagcaccga ataaacctca agttgatcct attacagagg gtgaccaaat tgttactggt
      961 aaaactgaac caaatgcaga ggtgacagtt acattaccag acggaagtca acaccatggg
     1021 acagctgata aaaatggtaa ttttacagtt aaagttccta aattagaggc agggacaaaa
     1081 gtgatagtaa ctgcaactga tgaatctggt aatactagtg aacctactaa tgtagttgtt
     1141 tcttccaaca aaaaagacag tggaaaaaac ggtagtaaag gtagtaagac tgacaatcaa
     1201 ggtagcaaca gcaatcaaga caaaaataga ggcaaaagcc agtcctcaaa agtttttcct
     1261 aagacgggag agagtgacag taatatcttt actattagcg gtggtttaat actgttagga
     1321 acgttaggat tattagggta taaaaatcga aaaaaagaga atgaatagaa aatcaatttc
     1381 ttaagtcttc tctgagaaga ggaagaatct ttttcttcct ctttttgctt ttttatgatg
     1441 ggaaataagt gttagttaaa gatgaatata ctttttatag aaaggaaaag aatgtaatta
     1501 agtggaaaaa atagtaatta ttggttcaaa ccattcaggg ctagccgcag ctcgatttct
     1561 aaacttaagc gattcaaatt atgaagttat tctaatcgat aaaaaatcag aaattagtta
     1621 cttggaatgt gggacatcgt tatttataaa aggagaagta agtaatttag aagatctttt
     1681 ttatcaaaat agaatgacac taaattctga gataaaaaag ttatttctag agacagaagt
     1741 tgacaaaatt gatattcaaa ataaaaagat tagttgtgta aaaaaagatg gtacaagatt
     1801 ctctcaagaa tatgacaaag ttatattagc tacaggttca aagcaaatta tgctagatat
     1861 accggggaat aatttagcta atattttttc gataaagaat tttccaaatg caaaagagat
     1921 ttttaaagct ttggatgata gtactattaa aaaagtagga attattggag caggatatat
     1981 tggagttgaa attgctgaag ccataagaaa aagagggaaa gaagtatatc ttttcgatgt
     2041 tgcagataga gtgttgtcga cttattacga ccgtagtttt tcagataaag ttgaagaaat
     2101 actatcaaaa aatggaatac atctttgtct atcagaaagt cctaaaaaat atttaggaag
     2161 aaaaaagatt gaaaaaatag tcactacaag tggtacccaa tactctatgg atatggtcgt
     2221 acaagctata ggatttctat ctaatagtcc aattggggaa aaggaactct tgagagatgt
     2281 tagtggagca tatctaacta atgaatacca gcaaacaaat ataaaagata tatatgctat
     2341 tggggattgt gctacaactt tttttacgtc cattaataga agtagtgttg atttttctat
     2401 ttctaatgct ctaagaacag cttatattgc aactcaacat attaacaatc aagattttac
     2461 accttctggt tcccaattta caaatggctt ttctatattt aattacaatt tttacgcagc
     2521 aggtttgaat ttaaaaacag cacgattatt aagtgagtct atagattata tagaaataga
     2581 agatttcatt agacctgctt ttttgagaaa aaatagtcgt gtaaaattaa gaattagctt
     2641 tgaaaaaaat acaaagagaa tagtaggtgt acaactttgt agtcaagaag atttatctgg
     2701 actattatca atgttttctc ttgctataga agaacaaatt acattagaca aattgaaaat
     2761 gttagattat ttatttcatc ctagttttag ccaaccctat aattttattt taaaggcagt
     2821 aacaggatta aacattgaaa cataatcgtt actaaatgaa atcaagaaat atactgtatt
     2881 actcaattca ttgaaaatat ttatcataat ttttaataga aaacactatt aaagagaaaa
     2941 aagagaggta aatcaaatga aaaaatcttt aaaacttgca tcagcagtta tattaatgtc
     3001 ccaactcctt atagcaacac ctttccatgt gttggcaagc gaaaattcaa catacaaaat
     3061 tgcaaaagaa agaaccgaga cgactgaaat gagtaattcc gatgtaacag cagagagtag
     3121 tgaacttttg acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
     3181 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcatcaag
     3241 tgaaagtagt acgacttcaa atgccgagtc ttcaacaact tcgtcaagtg aaagtagtac
     3301 gatttcaagt accgaatctt caacaacttc aagtagtaca agtgaaataa cttcttcgaa
     3361 agtgacaaaa ccaaaccata tagaatcttc aaaaaaggaa gcgccaaaaa cggaaggaca
     3421 aacaaaaact ttttcgacac cacagcaaat agaatcaaca gaatctttcc aatcacaacc
     3481 aatactatct ggtaatgaag aaattcattt tgacaaaaat gaatctgtag aaaattttat
     3541 tatgaaaatt ggtgagtctg cgagaaaaat tggtcagaaa tatgatttat atgcttctgt
     3601 tatgattgca caagcgatct tagaatctgc cagtggtcaa agccagttag ctcaggcacc
     3661 gaattacaat ttatttggga taaaaggaac ttataatgga aactttgtca ttatggtaac
     3721 aaacgaagat ttgggtaatg gaacgcttta tacgacccaa tcaaaattta gagtgtatga
     3781 aaactatgag gaaagctttg aagattatgc caaattatta actaagggaa ttagtggaaa
     3841 taaagatttt tatgcaggag cattaaaagc taatagtaaa acatatcgag aagcaactaa
     3901 attccttaca ggacggtatg caacagatac acaatactat ttgaagctaa atgaattaat
     3961 taaaacctac gatttaacca actatgataa agaagttaaa ggtccacaaa tgagtaagac
     4021 tggataccaa gtgcctattc ataattattc aataaccagt ttgtttggaa atcgtggaaa
     4081 tgaatttcac cgaggacttg atatggcagc tacacagggt gagccgattc atgcagttaa
     4141 agctggtaag gttgttaaag cagagtttca ctactcttgg ggaaattacg tggtgataca
     4201 acacgaagat ggatcaaccg cgttatatgc tcaccagcaa caatacctag taaaaactgg
     4261 agataatgtt ttgcaaggac aaataattgg ctatgtagga agtactggga atagtactgg
     4321 cccacacctt catttggaaa tttgcaaaga tagtagtctt tctcagagca tgttagttga
     4381 tccgaaaacc ttattattcg gagactaaaa aaggtaatca atctgacaaa gtaacaagaa
     4441 aatggttgga ttcttagacc tttgatcagg atattgagac tggaattgat atctcgatta
     4501 cgatgggttt gacaaataaa acaagtccat tatcgaatat cgagtgtatt attgccgtct
     4561 ttatgctcat attttgagca ggtttcacta gatgaaaaca atttatctat cttgaggatt
     4621 tcgtgtacgt atcgttcagt cttgtattgt aaattagtca taaaatcaga ctaagaaaaa
     4681 ttggaaatat ttttttctga tttattaaaa gttaattgaa attatatggt acttctttct
     4741 tgggagggta ggtgcagtga aggaaagata tggtcacctt actgttgttg aggagattac
     4801 tcataatgga tataaaaaat atcgttgcct atgtgactgt gggaatgaaa cgattgtata
     4861 tcgatcaaac ttactatcag gtagaaccaa aagttgtgga tgtggaaatt ataaaaatag
     4921 gcataaatat aaaaatttga caaataaaac ttttggtaag ttagttgcaa tcaagccaac
     4981 taaactgcga agtgaaggaa ctgttgtttg ggaatgtcaa tgtctgtgtg ggagaaaaaa
     5041 gttagtatcg gcaaagcgac ttctaaaagg tgatgtaaaa agctgtggat gtttgaaatc
     5101 aaaaaagcat cacattgaaa ataaaaaatt cggattactt actgcaaagt atcctttgga
     5161 caataactgt tcatggaaaa caaaatgggt ttgtcattgt gagtgtggca gtatgtgtga
     5221 gataagttat agtaacttat tttttggaca tactcaaagt tgtggatgtt taaaaaggca
     5281 agaaacaagg acatgtgttg aagggacagt aatagagtgt ctaacgtcaa aattaccgaa
     5341 aaataataca agtggtgtta agggagttta tcgttctaaa gagaaataga ttgcgtatat
     5401 aactttaaaa aaaaagaggt actatttagg atcgtatgat tctattaaga gtgcagctca
     5461 agctagaaaa gatgctgaaa ataaattgtt tcaacctttg attgacaaat atcgaggtaa
     5521 tcattaatta ttgtggataa taattaacag tgatatataa acatctataa tatattattc
     5581 atactattga tatttagtat aatcctatgg aattgaatat catagcaatg tatttgaaaa
     5641 gcaaattgga aaacaaatag gtgcaacacc ataaatatga aaaaattggg aaaaagtaga
     5701 atggaaattt gcttacgtct cacgagatag ctcacactat taatctgaag aacaatgaag
     5761 tcgactttta gaaattgcta gaagaaagta aagcgaatta tcactagtta ctatagagta
     5821 agttcaaata aagcatctcc aacttacatg gttacatgag gatatcaaat cgaaattact
     5881 caagcttcct atggctagag tcgcgagaag ttgttgggca gtagacatag gttatattgt
     5941 tcttagctac acgatcatta gaaaggaaat cctattatgt atagttatca agaagatgca
     6001 aaagtccaaa aaaatcgctg gtggattttg attgctatat ctatgtttac cttcatgtcc
     6061 acattggatg gcagtattgt caatattgca ctacctacca tctcagatga tatgggcata
     6121 ccaatgaatc aagcggaatg ggtagtgtcc gtttatctga tgattgtctg ttcttgtttg
     6181 ttgctgtttg gtaaaattgg cgatagccgt gggaaaatca aagtatttcg gattggtacg
     6241 ctgatctttg tcttgggatc gctgttgtgt gggttcaatc attcgctagg tattttattg
     6301 ttcgcacggg tggtacaagc cattggtgct agtatgacta tggctacaaa tgccggaatc
     6361 atcacggaag tctttcccat acatgaacgt ggtcgggcat tgggagcaat aggtgcgttt
     6421 gtttcattag gctcgattgc tggaccaggt cttggcggct tgattttgtc ccaattttct
     6481 tgggcgtaca ttttttggat caatgtaccg gtgggaatcg taacgatgtt gattgctgaa
     6541 aaattcttac caaaagatat caccatgagc ggccatacaa tcgatggagg gggctttgct
     6601 gccattgcgg ctacgattat cagctttttt ggagcaatct ttttggggca agaaagtggc
     6661 tttttccatc cagtccccct tgtactgttc gtaatttcag tgattgcctt tgtcagcttt
     6721 ttaaaagttg aaaagaaagc agcctctcca ttgatttctt tcggtatttt taagaacaag
     6781 atttttacca tgagcttgat tacagccttg ttgatttttt cttccaactt ctttgtcaat
     6841 gtagtgattc ctttttatct gcaaaatgct cgcgggttga gtcctagcta tgctgggctg
     6901 ttgatgatgg tctttccttt gatgatggtc gtcggggcgc ctttaagcgg ctacctgacc
     6961 gaccgggttg gcccggagat tttggtatta gtaggcttgg gcttattgtc ggtaacccag
     7021 gtcatgtata tgtttttgtc agtggactct cctttgtggc tatacattgt cgctactgcc
     7081 atcatgggtg ccggcaatgc cttgttccag tcccccaata atacgatggt tatgagctcg
     7141 gtcactatcg aaaacttggg actggcaggc tcgatgaatt cctttgcccg aaatttgggg
     7201 atggtcagcg ggatttcgct ttctacgacg gtcttatatc aagcgatgtc tgctaagatg
     7261 ggccagcaag taactaacta tatcgcaaat cggccggatg tctttttata tggaatgaag
     7321 attacctttt taggcagctt tgttttgtgt tttgttgcct ttttcctgac tatctggcgt
     7381 atcaaaaaac aaggaggtat tcagaaaaat gtctgaaaaa accactgttg aagaattttt
     7441 ggaggcctgc gtatgggtga gtgggatttc catggatgat ggaagatgaa gtgaacttct
     7501 aaaagccgaa aaactcaagt attatcttca aaatactagt gctcaatgag atgcttgatt
     7561 tgatcacaag aagactgcga aagccgctgg caaccgcaag ctaggtatcc caccgatgga
     7621 ctacgtttta gaggctactg gttatgaata cggagccaac gctctcatct gcataagaat
     7681 acatcaccca gattatgtaa agattttcga tacttctgtc aaaaaatcat taagatagat
     7741 aaatgatttc cgtctagtca aattggttca gaatgtagac acacagatgg taagaagtca
     7801 ctcacaattc gagaatggac ttgtcctatt tgccgtatac atcataatcg aaacgtcaac
     7861 gatagtatca atattttgac caagggctat gaatacaagc attgccttag aaaacatcaa
     7921 gaaccttagg aacaacgggg atagcttggt taataagaga taccgctgtt taggcttgca
     7981 agtatactct tttatcaaga attccgtggc tttagtcatg agaggcttaa tactatgaaa
     8041 attttcttta gcatttttga tttaggttcc ttttctaatt ataataagat gaccactaca
     8101 gtaacctact gaaatatata aacagccact gtagtaccat tttcaaagta gtctatgaga
     8161 ggagaatctt cattcttatg aacgctggaa ttgcggatat gaatttaata aaaaaaaccc
     8221 ttaatgattt tacttccaat tcaatatcaa aaggtactgg tatcaattta agcacaataa
     8281 aaaagctgaa aagtggtgaa agatcagtgg aaaagctgaa tcttttagat gctataaaaa
     8341 taacggaatt tgctatgaaa aatggaaaag ctgagattga aatatggaga taattatcgt
     8401 ctagtgaatt acttgccact tagctgaatt taacgatcct tggtgcttga agggggagat
     8461 tcttgggaat agagcatact tgcaagccta tgtctttact aacaacgata tctcttatta
     8521 accaagctat ccccgtagtt ctaaggcttt tacttttatc taagcaataa tcgtattcaa
     8581 aggaaatttt tgacccttag aaagtctcat tttatcagac gtgagcttag gcattgctga
     8641 attttgttaa ttcggtttta ggttgtgctt tgagcataat atgaaagtat tctttatcac
     8701 gatttcattc atctaaggta atgtgatgtg attctgaaat tctttcaaag gcagtttttg
     8761 cataagctga cattacatca tcaatgacat gtttacaatg tttcactact aaaataaggg
     8821 ggtaaccaag aagaaaaact gaatgattat ttgtatctaa ttccatggta tatcaaccat
     8881 ttcctatata gactgattat gacatggatt aaaataaaaa ggcactatgt cttaattgtc
     8941 ggttttcgag ttaccgaatg gcattcatca cccacctata gaggatgggc gacttctgcc
     9001 tgaaataagt tacactactt ataacttcta ataaaattag attgagtcat tattatttaa
     9061 catcaacgat tttccagttt ccattttttt gtaatactaa tttgtactga aagtagctag
     9121 tggtttttga tacattatca aggtatttta tctttgtact cactagaaaa ttttttccgt
     9181 tttgtagata aactggattc tctaactccg aaaactttaa gtcaatattg attggtcgca
     9241 tcgcaccatt ttcaacataa tactccaatt ctttttcaga agctgatgga taaagtttaa
     9301 aaaatgtttc taaaaaatcg gtgatttctt ccgttgtttt ggcatcaatt tctgaatcgt
     9361 tttctacttg ttttgcttca tattttgcct tgtctggctt tccaatcatt gttggcaaac
     9421 tagttactaa atgattcctg ttttcatctt caaagattgt cactcgatat gcagaagaaa
     9481 ttgattgcgt cttttttccc tctgtaatat gttgctccac taaaaatgaa acgacaaatt
     9541 cttccgagtg tttttcaaca tctagaattt ttacagattg aacttcggaa ctcgtgggaa
     9601 tatctgctcg aaccatatct tggattaaag cgagaccgtt ttctgtaagg tactgttcga
     9661 gattactcat tctcttttca attgcttctt tgttattttt ccatgaaaaa tattctttcg
     9721 caaaatcctc tgtaaaactt tcaacgccag aagtattcac taccttctct tttataacca
     9781 ctttttcatg agttgtatgt tgatcaatgg cggtgaaatt tttataaacg ccaaaagcaa
     9841 gactacaaga caataaaagc caaagggcaa gcaccatttt tcgatgctta cccacggata
     9901 caactcgttc ttttttcttt tttgggctct tttccttttg attccgttca atttttatct
     9961 tcattctttt tcctcttttc tcttatttgt atcggccagc tccgataaga tgctgccgcc
    10021 aatagttttc cgttagattc gtccacccca ctggatctcc tgcatgaaac atgcgattct
    10081 cccctgcgta aatgcccaca tgggtcacat aagtaccggc atcataggtt gaatgaaaga
    10141 aaactaaatc acctggtttg gcatctttta gatcaatatg agtcatggca tcatattgat
    10201 cctgagccac ccgaggaagt ttcaggtcaa ctttggcata acaccattgg gtcaagccac
    10261 tacaatcaaa ccccgtttct ggacttgagc caccaaacac ataggccgtt ccttcatact
    10321 taaatgcctc atcaaaaatt tcttggacta ttttgtcatc aaactgagtt gtagttaggt
    10381 attgcttcac taataaacag taaaacatat tgccataatt ataccgccac ccaccatttt
    10441 ccttaatcgc gattgggttg gggtaatcga ctttttgacc acctgatttt tcctttgaaa
    10501 aagattcagc taaagcaaaa ctatgcttct tcccgttttt cccgacatga tctagatacc
    10561 cgccaccata gttataggcc tgaataatac tatcttgatc acaccctagt tctttggaac
    10621 gcttaaccaa agacgcaaaa tagagacagc cttgtttgat ggatgcctcg gtacttaatg
    10681 aattaggtgg taatccggct gactcagaac tttgcataac gtcttccatt gtgccaccag
    10741 attccacttg aataatcgcc aaaagaattg gaatatactc tgttatatcc gattctttgg
    10801 catacttttc caccattgca cggtgtttca atacttcttc tgagacacta gttccgccag
    10861 gagaagtgcc agaactacca tttccctcac tgtcttcacc aaacaacaaa cccacacaca
    10921 tcaaaatacc cataaaagag atggtcagaa aagttaatga aataacaccc actttcttca
    10981 agttcatttt ttctcacctg accttgattt tggacgatgg ttcattcttt tttgtacttt
    11041 ttgattggtc tgctttggca gagtggacct ctgtttcact tgttggaaag aatttttctt
    11101 acttgctgat tggcttttcc cttttgaaat gatttcttga ctagttagtt ttcgtttgat
    11161 ttcgggattc agatttgctc tcacaatccg ctttggtgct tcttttttca gcatcatatc
    11221 tttctgaggt tgagctgctt tatctattgg aatattcgcc cttctttttc cctgacgatt
    11281 tgttgcttca ctcagtatct tgcgtttttc gtccattttc gcatttcttc gttgtcgttg
    11341 tttttctcgc aaatccactc gatccgcttt ttcttggaac agtccgcgct tgaattcttc
    11401 tggtgctttt ttcgactttt cgattccttt atggagattg tatttcaaat tcgttggcgt
    11461 attccttacc tgatccttta cttgtttagt cttatctttc accaaagctt gggtatccaa
    11521 aactttgccg atttttctcc cagccaaatt cattcgggat tgtttcttat tatttgaaac
    11581 tggattctcc ttagttaagt cagatagtat ttcgtgatcg ttcggtaatc gtgaattctt
    11641 tcgtagagaa gaaccggatg gcttaaattt ggattttgat tgttcctttg ccaacttatt
    11701 tccgactgct gcaccaccac caaaagccaa agctcgtccc acatttcgat tgagttgccg
    11761 caattttcga ttcattaaca tttgcggttt tcgcatgacg cgtcttccca ttgactggct
    11821 atcattactc tggaggttga acatactcat aatgtcgccc aatttgaaat agataccggc
    11881 aaaggtcaca atttgcagaa aagcaaccat gaaaaatgga taggttgccg aaatattgaa
    11941 aaacatcgtg gagatgctaa atgcagtcgt aatcaccaaa gtaacacccg ctcgcatcat
    12001 aatcgtattg aataaacgaa taatcgcctt ctttcctaaa ctttcataag tcggcaacat
    12061 ggacaataag aaactaattg gcagaaacat cgcaaagata atgaagagaa tttgcgagaa
    12121 caacataatc cctgttagaa gaaaaacaaa gaaggaaatg ccaatattga acaataccaa
    12181 aaagaccacg gtccctaaac ggttcatggt cttggtaata ctgagattcg cattgtcatt
    12241 gtcttcaatc tctgccttga ccgcttcttc ccgatctttg cctttgtttt catctggact
    12301 cacagataga atcttgttga cccggtcttc accaatttct tctatgttgg aatcatcaaa
    12361 ttgcagtagt aaccaaggtt gttggacttg aatcgaaaac aaactatccc gaatcaaatc
    12421 cacactatct tttccttggc tttctgaatt gggtacaacg atctcggttc caagggtaag
    12481 ggccgcttca ctaacatctg agctaaagtc attgattttt gtgatatagg taggcgcata
    12541 agcaatgaac gaacccgata gtagaaaaat catcacaaaa ttaatcacgg ctcgaactgc
    12601 tttcgtggtt tctcgtttta ataatccggt atacgccaca tacaccccca gagctaaaat
    12661 catcaataat agaaacccaa aataaaatcc ggaggtctgg agtccatttt cagtaatacc
    12721 agccaatgtt tggatattct ttccaatact ttcagccgta tccgaaatga aatctaattt
    12781 gtaagcctct tgaaccacat aacccgtggc gttcgacaaa tacaaactca ccgtccaaat
    12841 gaaattagta atggcataaa gaccatacat cacgcttttc cctaagccat caccccaatt
    12901 ccaaggcaac caatcccaag agctatccac aaagaaatct agttgatagt tgttcagtga
    12961 atatttggaa tatagatttc ctgcttcaac tgtatcatca actagccctg tcgcctcggc
    13021 taccgtacca actaaactca atagaactat cagagtgagt atgctaataa aaacgattcc
    13081 cacaatccga aaaagtttct ttttcatccc ctcactccat tctctcttga acaggtggac
    13141 gggtatcaaa ggcatggaag agttcttcaa agactggatg tacctgaacc acgcctaccc
    13201 gaccatacaa atcctgcatc aagcattggc cattttccaa atctcgtagg cgtttttgat
    13261 tgctttcatc ttctcgatcc aagccaaaga attccaaggt tttcttgatt tcatgaatat
    13321 cggtactgcg aaacgcaaac ttcaacccga tattattctt caagcgttca tccagtaaat
    13381 catcactatt ttgcgtgaca aagtaaaccc cggcattcat agcccgccca gcacgaacga
    13441 gcttattcga taatgttttc ccttgcgcta cttgaagaaa actccacgcc tcatctaagt
    13501 ctacaatttt gaagatttct cgattactat ggataaagtc caaggcaaaa gtggaaatga
    13561 cgattagcat ggaaacacta agcaattcca tggtggtata ttcttcaaaa gtggtttctg
    13621 catctggtaa aaccaaatcc gctacttgaa tgatatttaa ctgcctatct aaactaatcg
    13681 actgggtaat cgaaccatct gagaaaagca aatgggcaaa atcataatcg gtaaaggact
    13741 cgatgtgatc cgcgatattt tccgcaactg gtgagccttc ttttcgaagc tcattaatta
    13801 cttgtaataa tccccgttga ttgctttggg taaccgtccg aatggcctta cgcaacactg
    13861 gaaacttctc cccatctcga ctagaaattc ctgttaaaaa ggtcagaata tcaattgcta
    13921 gactttcaga atccttcttt ttctccataa tcacataagg atcaagtaat ccttgatttt
    13981 ttggcttact agttaagttg agaatattga tttcatccgc aatttctggc aaggtttctt
    14041 tccagccacc ccgttcccct tttggatcga caatcacagc ttgtccacca aaaaggacgg
    14101 cgtaataaac taagagattg ttactaaagg actttccgcc accgagtgaa ccgaggaatg
    14161 ccgctgctag cgcattggta actgaatcct tgaccccttg tgaggccaat gccggtttca
    14221 ggtaaacatt gcgccccgta tctacgttat aaccaaagta aatgccttcc aactccccta
    14281 acatctgagt ggcgccaaaa ccaagtccgg cgagaaaatc agacgtaaca tactggatat
    14341 aatcattcat gtatcgctta ctagaaggta agaattcttc atgtagcccc atcatatcgc
    14401 caaaaggccg gactagtttt acattggtat catcataaaa atctagcact tcatcacacc
    14461 ggcgctttag ttcatctact gattcggcac ttactcgaac cacatagctc agtttgtaca
    14521 tggattcttt ggactgatcc aaggtagttt ccaattcatc tacggaatcc aatgcatcta
    14581 agacattgga atttgtttca ttgtcagatt gataggcatg gttgtccaag tcttttaatt
    14641 cctttttctt attgcgaacg gtagcaagtg cctttttgtt cgtcacaatc tccacattca
    14701 tactagtgtc aattgggaat gtaaattgtt gttgctggta atagaaaagt tctgaggagg
    14761 gaaactccat ttcccccaca attgtattaa ttgttaagta cgctacatat gattcatgat
    14821 tctcatgttc catgcataag taccggggct tttcttcaat caaacaacga ctgggacgta
    14881 ataagtcgta acgtttcacc aaagtttccg acttcagctt tttctttggt agctgaaatt
    14941 catattcttc aaatggtgta cccttttcgc catagatatg ttcaatcaaa tacatcagat
    15001 cactcggtgt gactcgtctg actttaaatc ttcgagccaa tttactttcc attagttttt
    15061 caagcttggt atatcggcga atttcttcat tggataagga aacaaaatcg cccatcaaat
    15121 gatgattcac tccatagaca aattcttgaa agccggagaa gaaagatttc ttgagattct
    15181 ttagattgac ctcttcatct gtcgcaatca gtttgaagcc aataaaaaag cgataatcaa
    15241 tctgactatc gccaatcatg gatactaatg cctctgtctg taaatcaatt cgttgtttcg
    15301 ctacttctgt taatcgcccg gtaatttcct tttttgatcg ttcttgcgtg gcacgaatac
    15361 tactttctgt ggcaatttgt aaggcatgaa tcttgccttc acgattttgc gcaatcaatt
    15421 gacgaaagtt atcgtggact tgatatttct gttcaggtga caaaaaggag taattgtaag
    15481 gtattaactc atagtaggca aaacattctc cctcttgatt aaagacaaga ttattttcaa
    15541 tatacttaat cgggtacata gttcacactc cttaccagag taatgggttc gtcccatcgt
    15601 ttcttctgtt ctttgacttt ctttcctgcg aaagtgacct taaatcgtaa gtgatagcta
    15661 ataaaagatc gtaaaaatcc caatggtttc ttaccgtcaa acgttttttg cgacatgaac
    15721 caagtaactg ccaccggtat acctaagtat tttaaaaagg ccccctctat catagatagt
    15781 ggaggcacat gggcaaatac gatgacaaac agcaaggata aaacaaacca agccatctgg
    15841 ttaaaagtca caggaaaagg cagttgaaag tcattaattg cataaatgac cttttcaact
    15901 gcccaaatac tggtgtagct ttttattttc ttcatcaaat cattccttta aaaagaagga
    15961 tagtttagaa atcaactacc cttcaataat tttgttaata tggatactca aaaacacctc
    16021 gactcgttac caaataattg cctccaatct ccaagtctct accacacgca tcaaaatcaa
    16081 tatacgtctt caagtgttct ggtacttctc caaaatcacc agcttctaca atcaagaaat
    16141 atgctacatc actcatatca tcgcaatctg gataatagat aatatcatcc acatgctcta
    16201 ccatctcctc aaaagaattg aagaaagcat gttgaatctc agaagcaact tcaccaactg
    16261 gcgttccttc aagttcctcc gctaagccac acaaccgatt aatttcctca attggcgtat
    16321 actcatcaat ctcaaaggga agttcataat catgaattgc atattcctcg tattcgtcat
    16381 ttaacccaat tcgttctttc acttcatcaa aatcgacagg tggggtaaac cacgccccca
    16441 ccaattcacc ttcgttatat ttcccgaggt tcgcaatata aacacgcatc tgttccatcg
    16501 tgcttccccc ttacgcaccg acaattcggt taaacaaact gagtaagaca tccttcaccc
    16561 cagaagcatt aaagaccaat ccgaccgcaa tcaaagcaat caccaaaaag ccaatcagtt
    16621 tggaaaactc ccgcttaaat cctaaaaaca aaccaatgac cacaattgcc agtaagacta
    16681 aggattgggc attggttaaa aaccattgat atatggatag gtaagttcct gatattataa
    16741 atatctcaaa cttttccccc cattcacacg gagcgtgcga ctttcaccgc actccgcgtt
    16801 ccatcgtgtt tatattgaaa agtatttatg tagtagacct atatcaagtt taattcttga
    16861 ttgccagcta atactctata tttatttagt cttatgagct ggactttatt tagcttcaat
    16921 agagctatat atttttgtat tgtttcttct gttttagcgt gtattagacg gtgtaccgcc
    16981 tcttttacca aaataagatt tttataatcg tcatttccac cagctccagt tggtacaata
    17041 tgatgacaat gtatttcatt agcttgttga aattcttcca gagttatagc acattttcca
    17101 tgttgagcag aaaatagcga aattcgattg tctgcgtatt ctattgtgcg attttccaaa
    17161 gataatttca ttaactggta catcagtaat ttatcaacac ttagattggt gtggataaat
    17221 tttcttccct ctagagtgta gcaattcgct aaatacacct ttgccattgg ttttctgtgt
    17281 tggatatatc caacagggaa aataggttcg ccctcgccag caacataccg aagcatttta
    17341 gatttaccat atcttgttgc ctcctttgtc gtaagttgtc tgcctgtttt tctcaatctg
    17401 ccccttttgt ttgtacctaa tctgttagtg aagatagcca ttacagctct attcagtttc
    17461 atacaatcca gatttacatt cgttgcaatc tgataataat tttgcatacc taataccatt
    17521 gaattataaa gtcttgtttc ttctaattcc gttttccctt ttgagggaga agcaattttc
    17581 tttgcctgtg ataccagctt gtctttttgg tttttgaatt gtttatcact tatatgtgat
    17641 ttaacaacta acttctttct tctcggtatc attttaatct tgaaaccaag aaagtcggag
    17701 tatctttttc taacatttac aattcttgtt ttttcttgtg atacttccaa ttttaatcgt
    17761 tgttcaatcc attgagtgat tgctattttg gctttttcag cactttcctt gtagcggcag
    17821 aaaactctaa aatcatctgc ataacgaacc atatacattt cctttaactt tgttttcttc
    17881 atttctttgt aagcgttgct tttacatggg ctaccgcttt tattgatatg aaccacatag
    17941 ttttttgtaa caggattttc ctgccattgg ctttctaccc aatggtctaa ctcattaaga
    18001 acaatgtttg ctagtagtgg ggatattatt cccccttgcg gagttccttt ctctggatat
    18061 gttattgtac cgttagtcat tttgattgga gcttttaata tcctttttaa aatgaatatc
    18121 aagtgcttgt cccgtattcc catagcccat atctgtttta tcagcttgga atggttcacg
    18181 ttgtcaaaga aacccttaat atcaaattca atcacataat gtagctggct gatttgtagg
    18241 cggttatagg ttgcttttat tgcattttca actgacctat taggtctaaa accatagctg
    18301 ttttcactaa atttagcttc gcagattggt tctaatactt gttttataca ttgttgtata
    18361 agcctgtccc acatacaagg aatacctaga ggtctggttt taccatttgg ttttggtatt
    18421 tcttttcgcc tcactggctt aggtcgatac ccatgtggac tgcctgcaac aatgtatctt
    18481 acttttttga caactgtttc tgctggcaat ttgccaatat ctttaattat tgtaccgtct
    18541 gttcctgctg ttttactgcc tgcatttgct ttgatatttc gatatgccag taatatattt
    18601 tctctcgata ggataatatc cataagatga gtgaaaatat ctccattctt acttttggca
    18661 tataaatcat caaaggtgtt ttgcatttcg taatattcag cgtgtcttaa atcttctaca
    18721 cacaatgttt catttttatt tttaggcata aggcttcact cctttacttg aaattgagtg
    18781 agctttttta tcttactcgt aatccttatt gaaacaacat aatacttttc taatatatac
    18841 acgacttgag ggcattcctc cattctcatt acaagaattt cttaggttcg ccctctgctt
    18901 ttcagcatta gttcaactgc ttattgttct tcgtcagttt attctcttga tgagaaccca
    18961 atacctttcc tcgttccgat aatcttatct atacatatat atccttaggt cactagctat
    19021 aagcctgctg gcttgatacc gcctgtaacg ttatatggca tttcataacc tatattctta
    19081 ctttgccaca ccagctacga tttctcgtac ctttgcattt ctacaaagtt caaatttaga
    19141 cccgtacatt cgctgtttcg tcagttcctt atagaacatt ctaaccatag atattttata
    19201 gacccccgac ctgttggata cgctacccac ctctggatag ttttcgatag aagatatatg
    19261 ttcatcaact attacggtat ctttcagccg tctttaccga gcttcaaaca ttctgagttt
    19321 cctcatacaa tgcttgtcgg aggctcaggg caggcgtttc agagcgttac ttcatcattc
    19381 cacctgtcag attatcagtt ctcctaataa ccaaactttt tcgttctttt attagtgcct
    19441 tagcttttca ctaaggaacg agtcgcacat aaattttgtc cgaaattcat cttgtcactt
    19501 tcctttcttt tccattgctc gatccgctgc tgcacttgca ttcatcaagc acatcacagt
    19561 aacaccaagc atactgccac caaataccaa aaaaatatct tttacaattc ccatgacgat
    19621 tcctcttttc atataatcaa attttcaatt gccatacttt gttgctccaa aatcttttta
    19681 tgtcggtctg ataagcgtgc attctgtacc atatcctgga tataggttgt ccgattcaat
    19741 cgatccaact ccttagccat tttccacgta ggggcgactt gtcggcctag ccaattcaag
    19801 gttcgttcaa tggtatacgg ttttggctcg gaagttaatt gaatctcttt ccgattccgt
    19861 ccaataaaac gcccccattg ttcattcaag ggccagttgg ttcgcctttt accttctact
    19921 ttatccgcaa atcgcaagta acgattgata atggaaaacg tggtgctctc aatgctacga
    19981 ccttttaaca aatcttgaat cgcatgaaaa gcacggtcat ttttcagtcg aatctcaaaa
    20041 cgatttttcg tctcagtttg gtctaatgga atccctaact tgatatattg ttcatagtct
    20101 ttttcataca aacagaaata aacctcactc ttgagactgc cgatatataa ggtattgccc
    20161 atgccgtcct tttcatcagc ctttaaaagt tccccggaac gataactttt aaaggtacga
    20221 aacaaggaga tacattcttc cttctgacat tttttcgtta gctcaggtat atccaatagc
    20281 cccactcgat cattgattgc caagtccaag cgtttcatca cgccaccggc ctttagacaa
    20341 tcctcgaaaa agtcgtacca gctacgcttt tgagcgagta aaaaagtttc aaattgtcgg
    20401 caacctcgcc cctttaattc taaaagcaca cctttatctt cttcatgaga caacatcacc
    20461 acaatatcac ccatgacata ctgttcttga taggagtaaa aggcataatc ttcatggagc
    20521 atatactcaa atttcaaatg gagaatttct tgaataattt ttcgtgcatc cgttgtggga
    20581 aagcgaatcc ttacatagtc aaacagtaac gtcaaaggca aatcgggatt aaagctttcc
    20641 agttggtcaa aaattttcct catggttttt atagatgcgg ttacgccacc attttctatc
    20701 tgatttaagt acggccgact aagtccacaa gccgtagcca atttattttg tgaaacgccg
    20761 tattcgttgc gtcggtcttt taatagccga taatctaaat tccgttgtgc caagttctca
    20821 cctccaaatt caaggtgtta actttttcaa atgagttaac acctatacaa attacttact
    20881 atctttgata caacaagcat tcttaatggt tgcttactta ttttttgtgt ttttgtaccc
    20941 ccctgttaga tacgggggtg agacatcatg gtcggctaac gccgtccatg aacaggcaca
    21001 gcaccacgga ggatttctgc ttcgcgaatc ctccgcggtc agcgcctaac agtcagttgt
    21061 gttggtttag gttactcatc tgtaatacca tttacttcca actctgcttt catttctaac
    21121 aagtattgac gaatatctgg ttcttttgct tggtccgcaa tccgctcaaa ttctgcaatt
    21181 acttgattta tttcaattcg agcatccgtc tcctcattca ttaaatatgg atcgccagcg
    21241 actttgacac cattcttgcg aatatcaaat tttaaccgtc gcaattgttc ttctaattct
    21301 gggtctgact gaatctttcc aatcacccaa taaatttctc caatcaactc atcataaacc
    21361 atgtacatcc aactctttta atcttctggc aatatcaaaa atgaccggaa ctgttacaga
    21421 gtttccggct tgcttataca actgactatc tgaattgact tgagaatctc gatcaaaagc
    21481 ccaatcggga aagccctgaa gtcgccaaca ctctctaggt gttagcttcc gaatttgata
    21541 ctgatccgtg acgatcgctt gttggtctcc tgttagtaag gtatgaacac tctgttttcc
    21601 cagacgacct cggcgacttg tggaattagg aaaagccaga ttcacactgt cttctggctt
    21661 cacctcttga tattctgttt cattttttcg aatcatgatt tttgtaccac cttgtttcgt
    21721 tgacaaggtg ggggcggttc cagtaacttc ataaactcga ttaccttgtt caaactttcc
    21781 cggtaatagt cctgctacag agattccatc ccttgctggc agtgcgattt tgattccttc
    21841 atcacctgtt aaggttggcg ctacgccatc tgaaccatag acttgtccac ccattcctcg
    21901 attggaagga ttgatattta ccaaatttct gatgttttga tcagatgttg aagacggtcc
    21961 aatgatagga aatacttgtt cggtacatgc gtctcgagaa tgtccgataa ggaatatccg
    22021 ctcccggctt tggggaatgt aggcctttga gttaagcacc taccactcca cgtcataccc
    22081 caatccatcc agcgttcgga ggatcgtctc gaacgtagcc cctccttcgt gattgagcaa
    22141 tcctcggacg ttctcaagga ataaaagctt aggtcggaga agagtggtga accgagcgat
    22201 ttcaaagaat aaagtacctc gagtatcgga aaatctttgc cgctttcccg caattgaaaa
    22261 agcttggcac ggaaatccgc cgcaaaggat gtccactggt ccgagggatt gaataaactc
    22321 atctgataca cttgtgatgt catgcatttc cacctcctta cttgtatcat gaattgcttt
    22381 ataactacgt ctagcaaatt tatcaatttc acaaaaaccg atacaatgat ggctcgcctg
    22441 ttccatacct aaacgaaagc caccaatacc ggcgaataaa tctaaaaatc tcatacatta
    22501 cctcattcat tgcaaagaaa aaagccaaga acattttgct cttggctcat acattattaa
    22561 tttgtcctac tattttttat ttaatccaac taatttccgt tgtttttcat tggcctgatc
    22621 ggctacatat ttaatcaccg ttttgaactc ttttgcagta gcattttccg ataaatccgc
    22681 aatgctctta ttgtatttct ttacaatttt cgttacttgt tctttctcag tcatgtaatt
    22741 gaacctccaa atctttccta ctatacccta gtggtaaaaa taattctaga ttatccgtag
    22801 cataatgaac ttcggaaata ttattcgtat aatcttttat tatgatatga agcaatacat
    22861 ctggatgttt actctttact tttgcaatgt tctctcttga tttgaaaaaa gcgttaataa
    22921 atgccttttt cggcacatga cgtccctctg caatttcacg ttcttgtgta aatttccaag
    22981 cgattttagg gtcttgataa acatagaaaa tcgtcactcg aaaatgtttt ttcaacgccc
    23041 gcgagatatt tttttcagca cttaatattg caaaagttgc atccagaata aaagaatagc
    23101 catgatctaa gacatatcta aatgcctgct ctaccaacca agaagatgct ttttgaaaga
    23161 gactagagtt ttttccatta taatcaggaa actgggtgcg aaagtaatcc gcatcaataa
    23221 tcacatggtt atcataattc tcagctaatc caatggcaac ctctgatttt ccagccccag
    23281 gactccctgc cataaaaact gcgtctcgtc caacttcggg ggatttccct ttgataatat
    23341 aagaaataaa cttatcctta tttgcttttg catactctaa ataagaatca ttactttcca
    23401 tattaattat cccctttctt cctactatat aaaagtatta tacgttactt gaagtataaa
    23461 gtcagtattg aagatcactc aatcattcat cttttctaat ttactaattg aatctaagaa
    23521 atcataccct tttggtacaa gtggcgtata aaactcacta atcacactgc cacctgtatc
    23581 cacataacct cgacctttga tgtgcttcat aaagaagttt ttttcgacct ccccaaacat
    23641 catggaatat ccgagttcac tcatacgacc caaagccacg cgaaagttga attgatcgcg
    23701 gattccgtct cctaaatatt tcgcatccgg cctttggcaa gccaaaatca gaaagaaacc
    23761 agattgacga cccaacatca cgatttgttt gagcttattc aaaatcaccg cactttcttt
    23821 cgtcgttaac atctccatat aagccacgta ttcatcaaag attaaaaagt tgggtggaag
    23881 tccaagatag gcataattct ctcctgtttt gtagttagac atttctttca ttgccttgct
    23941 acgagccatc atacgttcat agaaatcttc cacacaagca gaaatttctt ccttttgtga
    24001 ataaacatga ggcatcaccg tacctaaatc agctaaatct gcatttttgg gatcgaggat
    24061 aaacaattct gcatctgact tcaatagtgc ttcgataatg gtgaggagga aataggtctt
    24121 ccctccaccc gtgccaccag cgattaacat atggggtaaa gaatcataag cccatacttg
    24181 atttttcatc aatcgcagag tcccattttc tgccactact tcgtctatcc caattcgatt
    24241 ggcgatcata tcatacagta aagtatactc cacataggag tctttgagtt ccttttctac
    24301 taactcacaa tacagtccgc tttctaactt cttttccaat tttaaaagct gatcttggta
    24361 tttcccaagt gaaatttgaa cgcgaataga cagtaatcca tctttcattc gataacagat
    24421 tttggggaag taactgatag tctctctggt tcggctacta ttcaaatctt tgaaaaagcc
    24481 ttcactttga gtttgtttca cttcatacca atggttctct agaatcatcc gagctagctt
    24541 ttgtcgatgc cacaagcttc gccaatgatc aagaaaaaag tgaatataaa gcaatatcat
    24601 agctcctgaa aaagcgagac ttccaagcag acatactaaa tcttttatag accaaacgat
    24661 ttcaatagta gaaagtgata gcttagaaat atcaaattgt ctaaactctt gaaaataaaa
    24721 gacgctcatc caaccgatga acagagcgag tagccagcca agaacaaagt gataaaccaa
    24781 gtgctgatct cctgcgcgta tgcgatgtcc tttgtacatt ttgatggttt ccacctccaa
    24841 tcgaaaaagc ccaataggat ttcattaggc aatttcaaaa agttatgcta gtcatttact
    24901 gctacgagga ggggagttgt ttcgtttcaa ccaattgatt taaaaagcct tcaatagcat
    24961 ccggcatctc cacaccaatt tcttccaaaa tggattctgc ctgccgacat aagttttcat
    25021 ttacttcgac aactataaag aaagactctt cttctcctaa ccagtctagt acatcagagt
    25081 cttcttcact gtcgctattt tctccaagta aaccttgatg tataatcaga aacatttttt
    25141 catcagaggt taatgcttta acgagttgtt cctcagtcag ctcgcctttt ccatattgat
    25201 cgagacaatc taaaatatgt tcaacattaa tttccataat cttcacctat ttctttcttt
    25261 cattttggtt cgtaggattt ccttgattcg gctgccgctt caagactaaa tcatccgcct
    25321 taatgaacca attaacggta gttccttctc gaaaaacata attggcaacg gtatccacga
    25381 ttggattgac caactctacc tctgcatcat aagcaaactc gcgtaagggt acattagctg
    25441 ggatactaac ttgaatcatt cgcccttgtg cagaggattt taaattatag gttcgttctt
    25501 ttacctcatt accaattgtt ccatcctcat tttgcttccg tacttctcgg cgtaatgcgg
    25561 aaaaacgcaa cttcccaaat gttaaacctg aatctacgac aataccttct gttaatctca
    25621 tcttcttttc ctcctatgct ttcactaaat catcaacata caacacgtaa tcagtaaacc
    25681 cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct tttagcggtt
    25741 ggtcttgatc aaaatccttt tcaccggctt ccgcagctac taccacctca atatcatcgg
    25801 cccgttgctt actggaatac aaatgataac tgcgaccaat caccgtagta ttccgtccat
    25861 atccttctgt tataatttct ccttcaccgg cataacttaa ctttccaaag gtttcagcca
    25921 tatctggcac gacaaatttc aattccatct tcttttcctc ttttcattgt tagttttgga
    25981 tacaaaaata gcgcgactac ttttttagga ggcggtcgcg cacgttggtt cttaggattt
    26041 acttgtatca tctcatcacc ttagttccgg cgattccaga aataatagcc cgccgtcgca
    26101 aaaatcaaag taaaaccaat gaacagtaaa acattggaat ttttctcccc ggtttgaggg
    26161 aaggtcttca ctgtgggact agattcttga gagccacttg gaacagttgg tgtttccggt
    26221 tgtttcggcg tacttagtat ggttggaact tcttttggtg ttaaggtttg agatttttct
    26281 ttcaaatctt cattgtgttt tccattgata ttcccatctt tatcgtaatt gatttccgca
    26341 aaagtaaact tggttccttc tggatatttt ccggtatcta cttttttcgc aagcacagtt
    26401 ttggtgaatt ctttatcatt tacttcataa tcaatcttgc cagatttcca aatttctttg
    26461 tttgtaccat ccggtaataa agcatacaga attgtttcga aagcttcttt tgagccatcc
    26521 agtacatcat gagtaatcga cacatcatca aacatatcca ccacatcacc atgagtaaag
    26581 gtctgagaac cgttttctag gtgggccttt gtttggatag aaacatggtg ttccactgta
    26641 caactgacag tttgggcttg gttattcaag ctaacatcct tcgctactgg cttatcgcct
    26701 gcttcaaaag cttccttatt ttcatacaca tagttgaata agacaatagt tttatttaaa
    26761 acttgttcgg cggttaattt atgctgaatt ttccaagttc ctgttttctc tttgttcgcc
    26821 aacgttgcgc ttgtttctgc aatcaccact ggtttggctt tttcatcttt ttcttgggca
    26881 gcttttgtcg catccacatc aatggcttgc gctaccacat accagtcttc tttgatttca
    26941 tgtagattgt aactcaattt atcaactagc tcggtagaat ccagagaagt taattctttg
    27001 tttccgtctt tccaagtcgc aagagaagtt aaactatctt cttcctcagg caaatcatag
    27061 agcattaaac ggttcaaact aacagaaaat gctttgttcg tcagcttctc gtaaggaacg
    27121 gtcaccgttt taattggttg tttttggtct tgttcggtaa tggtaaagac atactcactc
    27181 ttcacaaagt cttctttgtt ttccttaaaa gtagaacgga tttctaatgg cgtaatcttt
    27241 tgaaaacctt ctggggcttc tacttcttca agtaaataat ccccataagg aagattttca
    27301 aacttgccat aaccatcaaa ccctaattgc tcgttataag ctgtggtcgc ttcatcttcg
    27361 gcacctgtga tttcattggt cccttccaat ggcgacactt taaaggtcag atcattgaag
    27421 ccagtttcgg tagtgcccgc agctgatcca gcaaatttaa agaaatcaaa gccaaagcga
    27481 atcacttgtt cttttgccgt aacatttcgg gtaatcacgg catttttttc gctatcatcc
    27541 acctttttga tggatacagg atactttgtt tcatcaaggc tatatccttc gggcgctttt
    27601 gtttcttgcc aataatactc gttgatagct aaatgtttaa cggcaacttg gttcttttcg
    27661 tctaaagtca aagtgaccgt ttcatcagaa cctttcgttc ccttcgctaa ctctggttta
    27721 aaagcctcac tccatttaac agcttttcca tccttagcag taaacaaggt gtattcagca
    27781 ccctcaaaga ctgccttacc ttgtgcttta tccccagtat ctttgtcttc tttcgtcaaa
    27841 gtggtttcgc cagtgacttc ttgattttgc ccttttacgt tactggtaac aggggctacg
    27901 gtttgattgg catacttcaa ttcgactttt acaggtttga acgtattcac gaacccatga
    27961 ctagctttag tttcggtcac ataataggtt cccaattcca acgcattagc aatctctttt
    28021 ggtgtttccg catgaccgtt ttcatccgtg gtcatttctt gaacgatttc accggttgga
    28081 ctgtctttgc gaatggcaaa cgtattacca gctaaggaat aattgtcatt ccaaagatca
    28141 ctccctgttt caactccgct tttatccaga atgatttgcc ctttttcccg tgcgtttttt
    28201 gaagtcacgt aaatggtttc gcctgcttta atggtggtag tcatgggggt ggtatcaatc
    28261 gtataaggcg ccggtactga tttttcggta atggttactt ttgtaccatg aggaatctca
    28321 tccaatgtag caatgccttc tttgtcagta gtcacgtctt ttgcaggtaa agtttttcca
    28381 aagtctaaat ggaaaaccgt tcctggtaca acatcacccg attctttatc gacttttttg
    28441 atctttaaag aaccctccgt ttccacatca attttgattg tgtaggtatt aggtttatca
    28501 atcgccccgg ccattaaagt ctgttggcca gcctttttat aggcgacagg tgttccggta
    28561 ccagcagatt tcttaagcgt cagcacacca ctttcttttg aattggcatt tggggtgatt
    28621 atcaactgat taccattcac acgataatca atgtttgcgg cattttccac aacctcatcg
    28681 aactctgaca aattcagatt attcgtatcc gtcacagtag tcgattgccc taatactgtt
    28741 ttagccgtac tattatggaa ggttggtttc ttctgataat cgtcaatggc ttgatttatt
    28801 tttgcctcaa tggctgcgat atttacggca ctaccatctg atcgttttat tgagtgaagg
    28861 gtatacccat taacttcttg ccagatcatc ttctgtgcca caatatgagt atctgcatct
    28921 gtacctactt ttttccataa aacggaaact aatttggctt tatcggacat atctggtaac
    28981 gggtttttct catagccagg agtgaactca ttgtaaatcg ggactcctgg ttcaatacag
    29041 aaaatatctt gtttaaagcc gttgtacact gcataatgcg gaacagtgac ttctgacatt
    29101 ggacgaccat cactaaaagt tccttcaaca gcatattgat gtgctaaatc tagttctacg
    29161 gttaccgttt gtggatgggt aatttcatca gcaaaagtaa tcgtaggagc aattgctcca
    29221 acaattgctt gaacagccaa actcaaaata gttaatacac ataatttctt tttttgtttt
    29281 ttatacatga aatgttctcc tttcaaatta agcacaaaaa aataagatgc tcgcttgaac
    29341 atctttgttt ataaagtctt tttaaaattt ttattgactg aatagaatgg atttgagaat
    29401 gaatgagcct aaaaatttaa tctaaaat
//
LOCUS       DAFCHQ010000027        29079 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000027 DAFCHQ010000000
VERSION     DAFCHQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.027"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..808
                     /locus_tag="LNC71_001263"
     CDS             68..808
                     /locus_tag="LNC71_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6466342.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="LNC71_001264"
     CDS             complement(1232..1402)
                     /locus_tag="LNC71_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6466343.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="LNC71_001265"
     CDS             complement(1596..1775)
                     /locus_tag="LNC71_001265"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466344.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="LNC71_001266"
     CDS             2162..3274
                     /locus_tag="LNC71_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6466345.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="LNC71_001267"
     CDS             complement(3352..3645)
                     /locus_tag="LNC71_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBK6466346.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="LNC71_001268"
     CDS             3813..4127
                     /locus_tag="LNC71_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBK6466347.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="LNC71_001269"
     CDS             4148..6127
                     /locus_tag="LNC71_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6466348.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="LNC71_001270"
     CDS             6140..6466
                     /locus_tag="LNC71_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBK6466349.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="LNC71_001271"
     CDS             6482..7000
                     /locus_tag="LNC71_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466350.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="LNC71_001272"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="LNC71_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK6466351.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="LNC71_001273"
     CDS             complement(8436..9941)
                     /locus_tag="LNC71_001273"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBK6466352.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="LNC71_001274"
     CDS             complement(10033..10623)
                     /locus_tag="LNC71_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6466353.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="LNC71_001275"
     CDS             10802..12448
                     /locus_tag="LNC71_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6466354.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="LNC71_001276"
     CDS             12497..13216
                     /locus_tag="LNC71_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBK6466355.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="LNC71_001277"
     CDS             13695..14642
                     /locus_tag="LNC71_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6466356.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="LNC71_001278"
     CDS             14654..15574
                     /locus_tag="LNC71_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6466357.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="LNC71_001279"
     CDS             15615..17072
                     /locus_tag="LNC71_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6466358.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="LNC71_001280"
     CDS             17417..18031
                     /locus_tag="LNC71_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6466359.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="LNC71_001281"
     CDS             18024..19748
                     /locus_tag="LNC71_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6466360.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="LNC71_001282"
     CDS             19745..21196
                     /locus_tag="LNC71_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6466361.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="LNC71_001283"
     CDS             complement(21669..23822)
                     /locus_tag="LNC71_001283"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBK6466362.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="LNC71_001284"
     CDS             complement(23824..24870)
                     /locus_tag="LNC71_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6466363.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="LNC71_001285"
     CDS             25020..26309
                     /locus_tag="LNC71_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBK6466364.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="LNC71_001286"
     CDS             26322..29024
                     /locus_tag="LNC71_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBK6466365.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9454 a         5025 c         5898 g         8702 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tagaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctttgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgattcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggcttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atacttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggac ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aattttaga
//
LOCUS       DAFCHQ010000028        27996 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000028 DAFCHQ010000000
VERSION     DAFCHQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.028"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="LNC71_001287"
     CDS             complement(105..629)
                     /locus_tag="LNC71_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466366.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LNC71_001288"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LNC71_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBK6466367.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LNC71_001289"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LNC71_001289"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBK6466368.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="LNC71_001290"
     CDS             complement(2351..3031)
                     /locus_tag="LNC71_001290"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBK6466369.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="LNC71_001291"
     CDS             3157..4026
                     /locus_tag="LNC71_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6466370.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="LNC71_001292"
     CDS             4052..4567
                     /locus_tag="LNC71_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466371.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="LNC71_001293"
     CDS             complement(4614..5207)
                     /locus_tag="LNC71_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466372.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="LNC71_001294"
     CDS             complement(5232..5411)
                     /locus_tag="LNC71_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466373.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="LNC71_001295"
     CDS             complement(5488..5997)
                     /locus_tag="LNC71_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466374.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="LNC71_001296"
     CDS             complement(6093..6473)
                     /locus_tag="LNC71_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6466375.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="LNC71_001297"
     CDS             6803..7552
                     /locus_tag="LNC71_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBK6466376.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..9090)
                     /locus_tag="LNC71_001298"
     CDS             complement(7873..9090)
                     /locus_tag="LNC71_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK6466377.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9348..10625)
                     /locus_tag="LNC71_001299"
     CDS             complement(9348..10625)
                     /locus_tag="LNC71_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466378.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="LNC71_001300"
     CDS             complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="LNC71_001300"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBK6466379.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11547..12491)
                     /locus_tag="LNC71_001301"
     CDS             complement(11547..12491)
                     /locus_tag="LNC71_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBK6466380.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12493..13179)
                     /locus_tag="LNC71_001302"
     CDS             complement(12493..13179)
                     /locus_tag="LNC71_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBK6466381.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13653..13973
                     /locus_tag="LNC71_001303"
     CDS             13653..13973
                     /locus_tag="LNC71_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBK6466382.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14127..15032
                     /locus_tag="LNC71_001304"
     CDS             14127..15032
                     /locus_tag="LNC71_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6466383.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            15029..15802
                     /locus_tag="LNC71_001305"
     CDS             15029..15802
                     /locus_tag="LNC71_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6466384.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15983..17407)
                     /locus_tag="LNC71_001306"
     CDS             complement(15983..17407)
                     /locus_tag="LNC71_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6466385.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17618..18064
                     /gene="nrdI"
                     /locus_tag="LNC71_001307"
     CDS             17618..18064
                     /gene="nrdI"
                     /locus_tag="LNC71_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK6466386.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(18129..20111)
                     /locus_tag="LNC71_001308"
     CDS             complement(18129..20111)
                     /locus_tag="LNC71_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6466387.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(20125..21021)
                     /gene="murQ"
                     /locus_tag="LNC71_001309"
     CDS             complement(20125..21021)
                     /gene="murQ"
                     /locus_tag="LNC71_001309"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK6466388.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(21024..22085)
                     /locus_tag="LNC71_001310"
     CDS             complement(21024..22085)
                     /locus_tag="LNC71_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6466389.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(22296..22862)
                     /locus_tag="LNC71_001311"
     CDS             complement(22296..22862)
                     /locus_tag="LNC71_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6466390.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(22890..24392)
                     /gene="eat(A)"
                     /locus_tag="LNC71_001312"
     CDS             complement(22890..24392)
                     /gene="eat(A)"
                     /locus_tag="LNC71_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK6466391.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(24650..25771)
                     /locus_tag="LNC71_001313"
     CDS             complement(24650..25771)
                     /locus_tag="LNC71_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6466392.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            25897..26838
                     /locus_tag="LNC71_001314"
     CDS             25897..26838
                     /locus_tag="LNC71_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBK6466393.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26988..27884)
                     /locus_tag="LNC71_001315"
     CDS             complement(26988..27884)
                     /locus_tag="LNC71_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBK6466394.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
BASE COUNT         8053 a         5879 c         5013 g         9051 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt gatcatttgt gtcgccagtg cttttttttg
     8341 ttcgagtaaa tcttggagaa gttcggcgta atagatcgtt ttttgacagt ggatactgtc
     8401 tttttcaaca gctggatagg cacactgtgc atagtggatg atttgatctg ccttttgctc
     8461 ggcacgattc gcggagattt ttttccgtgt ttcgttgatc agtttatttt ttatttttgt
     8521 ccgagtagat gccaatacaa aatctggatg gggaaaaaga cggatcaatg tcaatgcgta
     8581 aggggtcagc ctgtttgaaa agaactgttc aagctctgga aaagtcaatt gaaggcagtt
     8641 gtgtaaatcc atgcgcagac gagtgatttt cttctcaatt tcttgataaa aacgggctaa
     8701 atcgcgcatc tctaagtaaa aattgctttg tacttctttt ggctgacgtt gatttttaag
     8761 atgagtctgt gcgagccggt gggcatccga tttatctgtt ttccaactgc gtaaggtgct
     8821 ttcttccatc tgttttttcg cctctaacgg gtttaacaaa cagtaagaaa agtgatgatc
     8881 ttgaaagaac ttttcgactg gtctggaata aatccccgtt gcttcaaaaa caatcaatgg
     8941 acgctcaggc aagtgctgga tctcttcgag taatgcatga aatcctcttt gagtgtgttc
     9001 aatctcgtct tcaaataagc aggtgtcgtc tttgtatagg acactgtagc tgtgtcccat
     9061 tgaaacatct aatgcaagaa tgtaaggcat ttgttttcct cctttttaag ttttgaaaga
     9121 actttactgc ttcatttcga ttccaatttc ctatacacgg gctcatggcc ccaacatacg
     9181 taaacctgat tctaaaatga cagtaaagaa gttcggtttt tgatagggac tcgtagtcct
     9241 caaggctttg ttcgaacttt ctttcactct tcactataat ctaaaaatag aaaatagagg
     9301 agaacatcat tcgtcaatga tgttcccctc taatcttagt atgtttttta tagtaattcc
     9361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
     9421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
     9481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
     9541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
     9601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
     9661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
     9721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
     9781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
     9841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
     9901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
     9961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    10021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    10081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    10141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    10201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    10261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    10321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    10381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    10441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    10501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    10561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    10621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    10681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    10741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    10801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    10861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    10921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    10981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    11041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    11101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    11161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    11221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    11281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    11341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    11401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    11461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    11521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    11581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    11641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    11701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    11761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    11821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    11881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    11941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    12001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    12061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    12121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    12181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    12241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    12301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    12361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    12421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    12481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    12541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    12601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    12661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    12721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    12781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    12841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    12901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    12961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    13021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    13081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    13141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga
    13201 caatattttt ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat
    13261 agacactatt tttaaatatt tttcgttatt tttttatcac aaaaattatc ttacttattg
    13321 taaggtaaag ctttttttaa tttattgccc gataatatat agaaataagg tctagttact
    13381 gtttttttta aatgattaat aaataacaac ttctttctat gaaattgcaa agcattcatc
    13441 ttcttttccg ctaacgatat atccgtcagt gcttggtaca ctttactaaa aagtatgaga
    13501 ttgccgatat ctggataggt gtgctatact acagaaaaag atatacaatc tgccaccggg
    13561 taggataaag aggatgatca ttccattagg tctttgaagg gatgattttc ctctttttgt
    13621 gtattttttt atagttaagg aggaatgtaa gtatggcatg gatggaatta atcatagcag
    13681 gaattttgga agtattctgg tcaacgatga tgaaatggag tgatggtttt tctaaaatca
    13741 attattcttt ttacacagtg attggcatga ttgctagttt ttactttttg tcaaaggcaa
    13801 tcaaaagttt gccgatgagt ctctcttatc ctatctggac agggattggc gcagttggtt
    13861 cggtcataat tggtgtggtc ttgtttcatg ataagctgaa tctttccact tggttctttg
    13921 tggggatgct actcatcagt atcattggca tcaagattac tagcggccac tagacacttg
    13981 gaataagtac tgatcagaaa aaataagcct ttttatttaa tattcaattt ttttatgaaa
    14041 aaataagaaa taagtccaat gacctagcga cttctccact catcaggtgt atgcttgtac
    14101 tatctataga aaaggagtga atatttttga aaagtctttc ggtccaagaa ttatccaaga
    14161 actttggtca caaacaagcc ctcagcgatg tcagttttag cttatcccct ggcaaaatcg
    14221 ttggattagt aggtcctaat ggcgctggaa aaacaacgat catgaaagct attttaggac
    14281 tgatccatta ctcagatgga acaatctgta ttggtgaaaa tgagatttct ccctctagtc
    14341 ataaagggtt ggaacaagta ggagcattga tcgaataccc gggtatctat ccattcttaa
    14401 caggctatga tcatctgaag ttattctctg aaaccaacga tgtatctgct atcgacacta
    14461 tcgtgaatca gctcaagatg gaaaagtaca ttcacaaaaa agcgaaatcc tactcgttgg
    14521 gaatgaaaca aaaattggga attgctcttg ccttactgaa ccatccgtct atcgttattt
    14581 tagatgagcc gatgaacggt ttggatccac aagcaacaag agatgtgcga aaaatcatta
    14641 ccgacttggc tactcagggc acctctttct tgatctccag tcacttactc agcgagcttg
    14701 aaaaaatggt cgatgagctg atcctgatca atcaaggaaa gatcatcaaa caatgtacaa
    14761 tggctgaatt gaaccaaaca gcgcaagatt tcatcgttat ctctacaaca aatgatctag
    14821 ctgctcgaac gatcttgaca gaagctggat atactttgga agatcctgat aaaatcaagc
    14881 ttttaaaaga aaacgaagaa ttattaggaa ccgtgatcca gctattgtct gcacaaaata
    14941 tccatgtgac agacgtccag catctgacga atgatttgga atcttctcta ttggaaattc
    15001 tggattcagt aaagggggaa gaagaaaaat gacattgctt gtaaaacaag aattatttaa
    15061 attgatcaag aaaaagtcaa ctgctgtttt atctgttcta ttagttgttt tgctcatagg
    15121 aacagcactt ctagccaaaa aatacacgac gatcattgat ccagttgaaa tgactgctca
    15181 gctttttagt gccacatctt ggattgtctt tatcatgatt gcagctgcaa gcacgatcat
    15241 ttcgatggaa gcacaatatg gtacattgaa aaatctcctt tatcgaaagt attctcgagg
    15301 agaaatatta gtaagcaaat ggatcacatt agttatttat tctgtttatt tatacctttt
    15361 agctattatt gtcactgtgt tgatgaaact gattcttttc ccttcgattt ccttcacgga
    15421 gaaggtttct actggtcaaa cactgatcca aagcttgttc acttatactt tgggtagtta
    15481 tatcggttta tggctgattt taagtttagt gttgatgatt gcttgcttta tcaatagttc
    15541 cggcgcatcc atctcagcag gaatcgtctt ttattttgct tcgtcaatca tatctggtat
    15601 ccttattgca ctcatccaaa aatgggagtg gatcaaatgg aatccgatca gtatgttgaa
    15661 tctgcaaaac caaattggaa acgaagagat catgaagcaa ttgactcatc tttctactaa
    15721 tcaaatgcta ttcggtaatt tagcttacat cgttttgttc ttagcattag gatatctcgt
    15781 atttaagcga aaaaatgtat gatgactcat caaaaaagag gctgtgaaag agacgttcag
    15841 ccttattgcc gattggctgt ctcttaaaca ccgtttattc tgaaaaagga gctgtgacaa
    15901 catttgtgtc acagctcctt tcctagtatg tttttgaata aacgacaggc tcacaacctc
    15961 tctttatact tcttttatac ttctatacgt gaagcatccc agtggcaaat aaaaccattg
    16021 caatcactcc gaccatcgtc acacctataa gtaaaatctt atctaagtgg atatgttgta
    16081 caggctcgtt ttgctcctta cgtgctttga ggtaaaaata aatgcctggc aagaaaagaa
    16141 caagaacaag cattacttca ttaaaggcta cgactagcat agcccatact tgaaaggcac
    16201 ttgctaaaag ccctactaaa aattggaccc attctttccg ctgataagaa agtttcattt
    16261 ggtacaatcc taagaaaagc cacgtgaaga aaatagctgc tgtaccaagt gtgtaagcaa
    16321 agttataagc actgtcagtg aataataatg aaagtaaaaa gacattcgta cataacgtcg
    16381 tcaatacgat agcataagtt ggtgccccat ttttgttgag ttttccccat gctttaggca
    16441 aaagtccgtc ttcagccatg tttcccattg tttcaatcgg taagatcgtc catgagagcc
    16501 aaacaccaac taaagaaatg atcaaaccgc cgttgatcac gactgctccc catttaccaa
    16561 ccatcatttc aaacacatag cccatagcag gctgcgcctt gatcgtttgc agctgattca
    16621 tcgtcattgt cccatatgga agcatggaaa ccaagacgta aataataatc aagccgaata
    16681 tccctagaat cgttgctttc ccaacatctg attgtttttt tgctcgtgaa cctacgacag
    16741 aggctccttc aataccaaca aatacccaaa gcatcaccat gatgcaactc aatacttgac
    16801 tggaaaacgg gatcggctgt ccattcgcat tcaatataat gtcgctagaa aagttatcaa
    16861 tgaattgttg gatcgaaaac gtgctgatag ctactacaat aaataaaaat aatggcacta
    16921 atttacagaa tgtgacgata gcattgacga aactagcatt ttcgactccc cgattcacta
    16981 gccatgcata tccccataaa aagactgtac tcatcacgat cgatgccaga ttttgcccac
    17041 cttcaaatac tgggaagaaa tatcctaatg cgctcatcaa caatgttgca aaagctacgt
    17101 tgcctaacca gcatgtgagc cagtaacccc aaccgctgat aaaaccagcg aaacggccaa
    17161 aaccttcttc tgcgtaacta aagatcccgc cttttaaatc tggacgtttt ttattgatat
    17221 tattgatcga caaggagagc atcaacattg ctgttcctac gagtacccaa ctgaaaatag
    17281 ctgggccgcc agccgatccg gagatgtccg tcataatacc aaatacgcca cctccaatcg
    17341 ctgaaccaat tactaaggca gcaagtggaa ttaaaccaat tccttttttc tgttgctctg
    17401 tatccatgta agcatcctcc aaaataaaac cgcttttatc aaaaagtata acaaaccttt
    17461 tttattttta cttaattaat aaaaacttta atagaaatat aattctaccg gcttttttct
    17521 cattatacaa gcaaagcgcc tagtcccttt ccaaattaca ccaccaaaaa acttaaaaaa
    17581 gatgctatac taaaaggaaa gaattggagg atgatccatg aacattttat atatatcgat
    17641 ttcaggaaat acgcgtgcct ttgccaaaca tctagcagaa tatgctgaaa aaatgcatgc
    17701 agaagatcct gtcaatcctg aagttacttt aaaagaaatc catgaaaata gtgattttac
    17761 gaaagaaaca gaaccatttt ttactttcgt tcctacttat ttagacggcg gaaatggttt
    17821 agataacggt gatacagaaa tcttgaccga aacgatgcgt gaatatctgg aacatgaaga
    17881 caatcataaa ctttgtcttg gtgtagtcgg tagcggaaat aaaaacttca ataatcagta
    17941 ttgtctaaca gccaaacaat atgcacaaac atttggcttt cctttcttgg cagattatga
    18001 attacgggga acaccatctg atgtgacacg aatttatcag attttagcag aaaacaataa
    18061 ataataaaaa agaagaagct gaaaaataga tttcagtttc ttctttcgtt tctattgttt
    18121 ctatcatatt aagctttgac atccatcaca ggtgcttttg cttttgcttt acctgttttt
    18181 tccaagacaa agttttttac acgatccata ttggttacca agacgatcat ggatgtttct
    18241 ttgcctgctg ctttgatcgc ttctaagtca gcttctgcta caagcgtatc aggtgtcact
    18301 tgctgccctt ctgttacttt cacatcaaat ggcgtaccgc ctaagtcaac tgtgttgatt
    18361 cccatatgaa gcaagatttc taaaccattt ggcgttgtga tcccaatagc gtgtttcgtt
    18421 gggaagacag agctgactac cccagtaact ggagaataga tatgtccgtt ttctggttct
    18481 acaaagtagc cttcacccat catcttttgt gcaaaaactg gatcacttgc tttatctaaa
    18541 gaaacaactg ttccgtcagc aactgatgag aggatagtca cactttcagc tgaagtttga
    18601 ggtactactt tttcaggcac ttcatttact gtggctgttt cagtcgctaa ttcttctttt
    18661 tctaaacggt ctttaggaat accgaagaag aatgtagcaa cgaagccacc agcataagct
    18721 gccaataatc caaggatgta gcctaaccat ttaccgtcag agatcaatgg gatcaaagcg
    18781 gctcctgaag gtccaatagc gatagccccg acatttccga tcattccgat cactgccccg
    18841 ccgatacctc caccgataca agcagtaata aatgggcgtc ctagaggtaa tgttactccg
    18901 taaatcaatg gttcgccgat tcctaggatc ccgactggta aagctccttt gatcatttct
    18961 gttaattctt tatctttttt gcagcgaatc cataatgcca atgctgcacc cacttgtcct
    19021 gcaccagcca tcgcaagaat tggaagcaat agcgtacgac cagtttcatc aatcatttgc
    19081 acatggattg gagtcaaaat ttgatgcagt ccgaacataa ccatcggcaa gaataatgtg
    19141 cctagaacga agccggcaaa tgctccacca acgccaagta cccagttgat cccaccgacc
    19201 atgccatcag aaatgaaacc tgctaaaggc atgatgaaga aaatttcaat cagaccgatg
    19261 gcaatcaatg aaagagttgg tgtaacgatg atatctaccg catcaggtac tactttatgc
    19321 agttttttct caacgattga caatagccaa acagcgaaga tgacacctag gatcccacct
    19381 tgtccagctg caagagcttc accagtaaat aagtttttca gtggtgcttc cggattcatc
    19441 cctgtcagca tcacaacggc cccgataact ccaccaagag ttggtgttgc accaaaaact
    19501 tgtgcagcat tgatacctgt atagataact aggtagctaa acatcccgtt tttcaagatg
    19561 ttcatcacat cgatatattg ttgccatgtt gctgcatcaa gtgttccggc tgtgactaag
    19621 tttgctaaaa ctgcggcaat ccctgccacg atcccagtac cgacaaatgc aggaatcatt
    19681 ggcacaaaga tattagaaat gtcttttaat accgatttga ttttagatgg tttttgtttc
    19741 gatttttgtg ccgctttcat ttcttgtgcc cgttcgttta cacggtcttt tccagatgct
    19801 gatgaatttg ctgtagctgt acttacagaa gaaagttttt ctcctaattt cacgcctgcc
    19861 aaatcaacca tttgctgtgc tactttgttc actttcccag gtccgatgat gacttgaagc
    19921 tgttcatctt ctactacacc aagcactcct ggaatcgctt ttaattcaga gacatttaca
    19981 agatcatcat tgatgatctt catacgcaca cgagtcatgc agtggacaat gctttctaca
    20041 ttgcccatgc cgcctgcccc tttataaatt tgttttgcca gttcttccaa agtcagttcg
    20101 ttttttgcca tggtcttttc cccctcataa ggtccctttt acaaatccgt ttgcttcagc
    20161 taattttgct tgagcttcct ctttatcact atttgtcaaa atcatgacga tagccagctt
    20221 aacattttga tctgcttctt caaagtactt ttcagctgtt tcgtaatcag cttctgtcgc
    20281 ttgcatgatg attcgttttg agcgttcgat tagtttttca tttgttggtt tgacatcgac
    20341 catcaagtta ttgaatactt tacctatacc aatcatactg atagtagaaa tcatattcaa
    20401 gataagtttt tgtgctgttc ctgacttcaa tcgagtagag cctgtcaaaa attctggacc
    20461 tgcatcgact tcgattggga aatccgcgtg tttactgatt tcggcttgtt tattgcaaga
    20521 aatcgttcct gtgacagcac cgatcgattt tgcgtaatct aatccaccga tcacatatgg
    20581 tgtacgaccg ctagctgcga taccaataac tatatcattt tttgtcaaat tcagcttgat
    20641 tagatcttct tgccctagct cataagaatc ttcggcccct tcgacagcta cagtcattgc
    20701 ttcttctcca ccagcaatta gacctatgac catttccggt tctacaccga atgtcggtac
    20761 gcattcagca gcatctaaca cacctagtct tccacttgtt ccggctccca tataaatcag
    20821 acgtccacct tttttaaagg actcaatcgt gttagcaatc actggttcga tacttgtcag
    20881 ttctttttca accgcagcgg ccactttctg atcttctttg ttcattagtt gaacggcttg
    20941 agaaatactc atctcatcta atccaaaagt ttcttggttt ctgcgttccg ttgttaggtt
    21001 ctctaaattg attttgctca tttttatctt ccttcttttc ttataaattg atagttcata
    21061 ccagctttga tttggttgat caatggtagc tcttctttga taactttagc tactacattc
    21121 actttttcat ccgctggcaa atcatatttg gttaattgga tctcacccat atagcgcaaa
    21181 tacttttcgt tatctaaagt aatagaccct ttcatacgcg tagctgtatt tcttgctggg
    21241 ataggcggaa tctctcgaaa acgtgcatct acgcttcgta tgacatctct tgcatcgtct
    21301 tgccgattcg tatgcttccc taatatatat tcgtaaaatt cttcatctac aggttccgta
    21361 tggagcaaga ctgtctgttc tttttggaaa gaagcaaatt gctcctgaac gttttctgac
    21421 agacctgcat ctccgatgta aacaatatcc gtgttacttg ctaatagatc tagagcgcct
    21481 gcaagaggat ggatccctcg atgttcttcc aaagttggca atccttgata tagtgggcct
    21541 ctcaactctg tatctcctgg tacaaaacct tggacaagga gaccttgaat tttgagccat
    21601 tgattttttt tctgatacca ttctttgtcc agccctgttt cgggacgtgg ataataatta
    21661 tgccaagctt caatctgtga gaaatcagct tctgcttctt tcaattcatc gatatcttta
    21721 ggcgtgatcg tgctagcatt caaactgatt ttcaattgat gtgaccagtc ggcaatctgc
    21781 tgattggaaa tatgataatc cattcttaaa cctgttacac caattgcttt caaaggcgtt
    21841 agatcttcta atgaaaaacc cgctcgttcc aatgcctctc ctgaaatatc gaccatcaat
    21901 tctagtttat tctttttcgc ttcgcttcct agttcgatca atcgcttttg atatgccgaa
    21961 acatcatctt ctggtatatg catggaagtg aaaataccga caaagccatt ggatgccatt
    22021 tggcggatat actgtttctt ctcttctgac aaagaatcgt tcataaatat tgaaaagcca
    22081 aacatattat ccctcttttt ctgtatccgt cttcatttct atgactagag tatatatcta
    22141 aagaaacaaa atttcaatac ttttattaaa ataattaaat ttcatttcta aaggttgagt
    22201 ttatattatc aaaagaattg tcataaccta aaaaaggcac tgctaagcaa aagcagtgcc
    22261 ttgaaatatt ttatttaaat aacacaaaaa cataattact cataagaaac caattctgta
    22321 ttatctacca tgaatgtaaa gaacccctca ttaggcatcg gatgaaaata agcattgatc
    22381 actcttatca aggcattatg gcaaactaac agatagacat tttcttcgtc ttcgatgcat
    22441 tctttcaata aaggaacgat ccgtgcatac acatctaaga ctgactcccc atttgggaat
    22501 cgtacagcga acgctaaacg tgctttttga aagttctcat ctttgcttgg cagtccatca
    22561 taatccccaa aatccatttc taccaatcgt tcatcaacgg tcagcggcaa gctgagacga
    22621 tcagcaactg cttgtgctgt atctcttgct cgttgaagag gggaatggat gattttcgtg
    22681 attggaacag gcaaatctac taccttttct gctagtttct gtgcctgcat ctgtcctttt
    22741 tcagtcagcg gcacatctgc ccgtccgcaa actaatcctt gtgcgttcca agttgtttct
    22801 ccgtgtctcg ttacataaag catgtgattc tctccttcgt tggcaaagtt ccccttaacc
    22861 ataccttgtt gttcttgaga ttacaagggt tagattctct caagagaaat aatctcagta
    22921 gatactttat ccaggaaggt ttgatcatgt tccactagta acatggcagg tttcacgttc
    22981 aagatcagtt gttctaattg ttcttgattg aagacatcca aataattcaa tggttcatcc
    23041 catgtatata gttcagctgg ctgtgataaa gatttagcca attccacttt tttccgttgg
    23101 cccatactca tctgctcgat cttgttatga aaaacatctc tttccatccc aagctttcgg
    23161 aggttgttca aaaatgcttg gtagtctact tggtttttct ctgcaaattc cgccaacgtt
    23221 cctcgattgt cttcataatt ttggcttgca taactaatgc tcagatgttt tggctggtat
    23281 ttttctccta taaccttgcc gttgaatgcc cccagaagat aatggataat ggatgacttt
    23341 cctgcaccgt taggacctga aatcgccacc cgctgatgag gctcgattgt aaaatgaatt
    23401 ggctcgaata acagattttc ataccctaat tgaagatctt ctacgcttaa aagtcgctta
    23461 tggtgagacg cttggctatt catcgtcaaa gaatcgatat attcgatatc ttttaatagt
    23521 ttttctttct cgctgatctg ggtctccatc cgattcacga ttgctttgga tcgtttcatc
    23581 gtccgtgcag catcagcacc cactgctcct ttattcactc gtctagattc agtatcgatg
    23641 aatccgactt gtttctttgt tttatctcct tctcgggaac gagaccattc ggctttttca
    23701 gctgctgttt tctttagcct actgacttct tttttcaatt tttcattttg agccatttca
    23761 aattcatcac gaagtttttt ctgttcttca tagatagaga aattcccttg ataaagttcc
    23821 agttgacttt tttcgattgc taaaacatgg tccactactt catcgataaa tccccggtca
    23881 tggctgacca cgatgaagcc ttgttttttc tttttcaaat aagccgctac ttgttttcta
    23941 ccagagatat ccaaatgatt cgttggttca tcgattaacg ggaaatgagt gtcatccaca
    24001 aataaaagtg ccagtaggac ttttgtcttc tctccccccg atagtgtgct gaattctctc
    24061 cataagattt caggatctgt ttgcatcaat tggagctctc tttcgatttc ccatatctca
    24121 aaatccgtaa tatcatttaa cacgtaatag gttaaacgtt ctttatcttt tgtctgttgc
    24181 gggaaatagg caaattcttg ctgatggatt acttgccctt gataaggtag tttgttttgt
    24241 agaatattca gtaaagttgt ctttcctcga ccgtttcgtc cgataagtcc tagtttccat
    24301 tgtgtgtcaa aatttagatt tgcttgttca aataataatg tgccttggct gtcgtagccg
    24361 aatgtcagat tttttatttc gattttagac attttccatc acctcgtaaa aaaagaccag
    24421 tcctattttc agaactggct aaagaggatg caagggttgg atacacacaa aaagacccat
    24481 acatcgaaaa aaccaagcca atcccgaaaa tctgcacact gcttccttgt ctccacaagg
    24541 tacagtctga atgcagcttt catcaatcga cttaattttt caatgcacgg atccctcact
    24601 ttgtcctata tttgaaatta cgatagcata aagaaaatga ttttgtcaac tacttcggga
    24661 ggatggtgaa aaatctgcta agtgtctgtc ctaagatttt tgttttttct aatgtttgcc
    24721 tgtgttccat tgcttcttct aaacctattg gacccaaatg aatacttaag acaattggga
    24781 gaaaatcaga aagtccttct agctcttggt ctctacttcc gcaaatagcg attattggtt
    24841 ttcgatattt ttttgcaagc actgctacac cgtatggaac ttttccgtgg atcgtttgct
    24901 gatcgatccg cccttctcct gtaatgatga catctgcttg ttcgatcttt ttttccaatc
    24961 cagtgatttg gctgactaaa tcgaaaccag aaaccatctt cgctcccaac aaaaaaaggg
    25021 ctccaccaat accgcctgct gctccacttc ctggaatatc atctaaatcc acctcccaga
    25081 aatcttttat ttgctgagca atctttcctg cttgtttatc taagaaagct agcgtcgtat
    25141 ctattcctcc tttttgtctt ccaaattgcc aagcagctcc ttgttgacct gtatatggat
    25201 tggtaacatc tgcagcgata atcaatttta ctttagaaac aagaacgttt cttcgatcaa
    25261 aatgaagttt tttagtagat aataaaggat ttccttctgt gtacccttct aaagaagcac
    25321 ccaaggattg gagtaatcct agaccaccat cgctacaccc gcttcctcct aaagtgacaa
    25381 taatttgttc tacttcttga agcagtgcat ctttaataag ttctcctaag gcataactgg
    25441 aagcaagtcg gatagtttga tccgttggtt tatcaagtag atctaacccc aatgcttgtg
    25501 cagattcgat gactgcggtg ttttttcctt gccaagttgt caataaatag ctgatttttc
    25561 gctgacggaa taagagatcc atcccatcaa tggtacgcca tgttcccctg agcgcgtaat
    25621 agattgcttc tgcagtattt tctccgccat ctgcaatcgg cacattgatt tttcttcctt
    25681 tacgatcttc ccagccattt agaactgcct gatttagttc aaatgaagaa gcacttcctt
    25741 taaatgaatc aattgccgcc aaaatatcca tctgtatccc tcactttctt cttaagtaag
    25801 acactttttt tcattttttt caaacaaaac aatgctattt tcttccattt ttctatatac
    25861 taaaagggaa ctcattgaat aaaggagaga aaaaacatga aaatcgcaat agctggggcc
    25921 ggagcaatgg gcagccgaat cggattgatg ctacatcaaa gcggaaatga ggtcctcttg
    25981 atcgatcgtt ggcctgctca cattgaagcc attcgtacaa atgggttgat tgctgacttt
    26041 aacggtaaag aagtggtcgc aaaactgcca atctattcac cagaagaaat catcgaaagc
    26101 aacgaacacg tggacttgat cgtagcatta acaaaagcca atcaattaga tgatatgttt
    26161 tgttcgatcc aatccattat tactgacaat acttatgttt tgtgtttact aaatggatta
    26221 ggtcatgaag atgtactgga aaaatatgtg ccaaagaaaa atatcttatt tgggatcacg
    26281 atgtggacag ctggtctagc tggtcccgga aaagtcacat tgttaggtga tggagaaatc
    26341 gaactagaga acttggaacc agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt
    26401 caagaagcaa atttaaatcc gatctatagc cataacgttc gttattccat ttggcgaaaa
    26461 gcttgtgtaa atggaacatt gaatggttta tgcacgattt tggattgtaa tattgctgaa
    26521 ctaggcgcgc aaaaagcggc agagagcatg gttcgaacaa tcgtatctga atttgcttct
    26581 atcgcagcaa aagaaggaat catactagat caagaagaag tgtaccaaca tattgcttct
    26641 acttatgatc ctgacaacat cgggttgcat tacccatcta tgtaccaaga cctgatcaaa
    26701 aaccatcgtt tgactgaaat cgactacatc aatggtgcca tttggcgaaa aggacaaaaa
    26761 tatgatattg ctacaccata ttgtgccttt ttgacccaat tagtccatgc aaaagaagga
    26821 atccttggcg cagaataaaa ggctctctga tgagaatcag ctgaatcagc agctggttct
    26881 ctttttttaa gcccaataaa agatcaaaaa gaaaacacaa ctctcatgat gccataagaa
    26941 ttgtgcttgt ttgtttcagt acataatctc ttcaaaatgt aggtttttta tttgttttca
    27001 acagaggaga ggtcatattc gtattgctca taaatagtgg aatcgttagt gataaatcgt
    27061 tttgcccaat tttctgcatc ataaaattcc aatagctctg ataggattgg cttaaatggt
    27121 gtatgcagaa attccggtaa ttcatctaaa tcacgttctc gaagcaaaaa aatattttca
    27181 ggacgataaa aatgataatg agcaatcgct tgattatccg tcactagttt cttttgaaaa
    27241 tacatggatt caaatgtccg caaagtcact ccttgctgac cctcccgcaa cagttccaga
    27301 atcccgcgcg attcccctgt ttttttcagg tactcttcat aagtaagata tccaggttgt
    27361 tcattccctc gatgtggaag aatgaaatag ttggtcgtaa catctctttt tataaattcc
    27421 tgcttatagc tttctgctcg ttcttttctc ccattattcg ttgcaccaaa aaatagatcc
    27481 gtctttggtt cgctagaaga aaaattcata tgtttcgaat aaaatgaact attatgtttc
    27541 agcccaagtt cttttgcttc caaaaaatca aaatgataaa tctcatcgat tgccggttca
    27601 tcttgtgcct gcttcagcat cgcctcgtgt tctgctttga agtgattcca aaaaaataac
    27661 acaatttttg cattggtaaa tgggcgcacg cgtcgagcca tctccatatc aaatccataa
    27721 tctaaaaaaa cgatcagctg ataatttgcc aactgttgtc tagctgattt aatgaattcc
    27781 gactgtagta aatcttttcc ggaccaaaca tctaaagagc ggatggaagg caaaaaataa
    27841 agtgatttat tttttgcaac tagcagtacg tcatttttgt tcatcttttc cgcctcactt
    27901 tctctgtcac tttttcctat gatagcctgt ttaaatagta aaaagccagt aaaacgtttc
    27961 ctatatttgt atgaaaattt aacatttgta tgaaaa
//
LOCUS       DAFCHQ010000029        26892 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000029 DAFCHQ010000000
VERSION     DAFCHQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.029"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(104..1336)
                     /locus_tag="LNC71_001316"
     CDS             complement(104..1336)
                     /locus_tag="LNC71_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBK6466395.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="LNC71_001317"
     CDS             1591..2160
                     /locus_tag="LNC71_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6466396.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="LNC71_001318"
     CDS             complement(2338..2778)
                     /locus_tag="LNC71_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBK6466397.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="LNC71_001319"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="LNC71_001319"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBK6466398.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="LNC71_001320"
     CDS             3732..4655
                     /locus_tag="LNC71_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBK6466399.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="LNC71_001321"
     CDS             4731..5870
                     /locus_tag="LNC71_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBK6466400.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="LNC71_001322"
     CDS             5863..6663
                     /locus_tag="LNC71_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6466401.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="LNC71_001323"
     CDS             6663..7490
                     /locus_tag="LNC71_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBK6466402.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="LNC71_001324"
     CDS             7468..8202
                     /locus_tag="LNC71_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6466403.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="LNC71_001325"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="LNC71_001325"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBK6466404.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="LNC71_001326"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="LNC71_001326"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBK6466405.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="LNC71_001327"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="LNC71_001327"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBK6466406.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="LNC71_001328"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="LNC71_001328"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBK6466407.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="LNC71_001329"
     CDS             11787..13820
                     /locus_tag="LNC71_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466408.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="LNC71_001330"
     CDS             13864..15144
                     /locus_tag="LNC71_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBK6466409.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="LNC71_001331"
     CDS             15354..16160
                     /locus_tag="LNC71_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466410.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="LNC71_001332"
     CDS             16172..17392
                     /locus_tag="LNC71_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466411.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="LNC71_001333"
     CDS             17382..18968
                     /locus_tag="LNC71_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6466412.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="LNC71_001334"
     CDS             19007..21145
                     /locus_tag="LNC71_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6466413.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="LNC71_001335"
     CDS             complement(21513..22496)
                     /locus_tag="LNC71_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBK6466414.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="LNC71_001336"
     CDS             22874..24265
                     /locus_tag="LNC71_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK6466415.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="LNC71_001337"
     CDS             24281..25321
                     /locus_tag="LNC71_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6466416.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="LNC71_001338"
     CDS             25340..26425
                     /locus_tag="LNC71_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBK6466417.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26892
                     /locus_tag="LNC71_001339"
     CDS             26458..>26892
                     /locus_tag="LNC71_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK6466418.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDY"
BASE COUNT         8638 a         4458 c         5453 g         8343 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    26701 ttacaaaatg agaaatattt aaaatatgta ttattatctg gtgctgatta tccaatcaaa
    26761 gataatgagt atatttataa ttattttaaa aagaatagtt cagtggagtt tattcgtgga
    26821 atagatctag atcaaattaa acataaagaa ttttattata aacatataga tgtatatcaa
    26881 aaacatgatt at
//
LOCUS       DAFCHQ010000030        26863 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000030 DAFCHQ010000000
VERSION     DAFCHQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.030"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(164..388)
                     /locus_tag="LNC71_001340"
     CDS             complement(164..388)
                     /locus_tag="LNC71_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBK6466419.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="LNC71_001341"
     CDS             complement(586..2166)
                     /locus_tag="LNC71_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBK6466420.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="LNC71_001342"
     CDS             complement(2567..3943)
                     /locus_tag="LNC71_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK6466421.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="LNC71_001343"
     CDS             complement(4071..5189)
                     /locus_tag="LNC71_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6466422.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="LNC71_001344"
     CDS             5331..5765
                     /locus_tag="LNC71_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466423.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="LNC71_001345"
     CDS             complement(5843..6376)
                     /locus_tag="LNC71_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBK6466424.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LNC71_001346"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LNC71_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBK6466425.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LNC71_001347"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LNC71_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBK6466426.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="LNC71_001348"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="LNC71_001348"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBK6466427.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="LNC71_001349"
     CDS             complement(10101..10679)
                     /locus_tag="LNC71_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBK6466428.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="LNC71_001350"
     CDS             11001..11471
                     /locus_tag="LNC71_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBK6466429.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="LNC71_001351"
     CDS             complement(11579..12805)
                     /locus_tag="LNC71_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6466430.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="LNC71_001352"
     CDS             complement(13559..13759)
                     /locus_tag="LNC71_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6466431.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="LNC71_001353"
     CDS             complement(13893..14501)
                     /locus_tag="LNC71_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBK6466432.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="LNC71_001354"
     CDS             complement(14586..15056)
                     /locus_tag="LNC71_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBK6466433.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="LNC71_001355"
     CDS             complement(15104..16018)
                     /locus_tag="LNC71_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6466434.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="LNC71_001356"
     CDS             16164..16967
                     /locus_tag="LNC71_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBK6466435.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="LNC71_001357"
     CDS             17145..18092
                     /locus_tag="LNC71_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBK6466436.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="LNC71_001358"
     CDS             complement(18160..18453)
                     /locus_tag="LNC71_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBK6466437.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="LNC71_001359"
     CDS             complement(18481..19146)
                     /locus_tag="LNC71_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBK6466438.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="LNC71_001360"
     CDS             complement(19286..19918)
                     /locus_tag="LNC71_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6466439.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /locus_tag="LNC71_001361"
     CDS             complement(19925..20992)
                     /locus_tag="LNC71_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6466440.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /locus_tag="LNC71_001362"
     CDS             complement(20989..21720)
                     /locus_tag="LNC71_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBK6466441.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="LNC71_001363"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="LNC71_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBK6466442.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="LNC71_001364"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="LNC71_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBK6466443.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="LNC71_001365"
     CDS             23883..25052
                     /locus_tag="LNC71_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBK6466444.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(25319..26233)
                     /locus_tag="LNC71_001366"
     CDS             complement(25319..26233)
                     /locus_tag="LNC71_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466445.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(26291..26863)
                     /locus_tag="LNC71_001367"
                     /pseudo
     CDS             complement(26291..26863)
                     /locus_tag="LNC71_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8207 a         5312 c         4281 g         9063 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac
    25261 aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc
    25321 attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca attagcgtga
    25381 ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg attgttactt
    25441 tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt agtactgtag
    25501 cattttttga attaacagta accgttaatc ctttataccg gaatacaggt aaaactattg
    25561 ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga gaaaagctac
    25621 tataaataac ttgaccatct tctgtaactt gatggttttt caaaagcaat tgatcttttt
    25681 tatagcctat aggtacataa tcaaaattct caaattcttc aagtttattt tccatttgtt
    25741 cattagaata agttccttga aaattcattt ctttcaccat gttaactact gatgaataat
    25801 ttaaaattga aatagctaat attaataata gtataaaatt tcttctcctt gtataagaaa
    25861 atttttccaa ctgtggagaa ataaatagag atactgaaaa agatatgaac aatgttgaaa
    25921 atatcataaa acgccaagga gcttgaataa tatttaaagg agtattttgt aaggcaaacc
    25981 atggaaatat ttttgtacaa agtagaatag atattatacc cattatataa gtgattttag
    26041 ataagattcc taatttttta aagttaaatg ttagtataat taaacaaata agcaaagtta
    26101 agccaatacc aaatgatagt aattctgaat ctatacttcc aatcaaaaac tctttaccca
    26161 gataaattgc tcttttttgt aatatatcca cataaggtct attcagatct tgatacaatg
    26221 tctgctcaat cataggacct ataaacgcta gtgaaagaag taaagtaact atagcagctt
    26281 ttattaattt aataattaag aagatttcta ttttcttttt ccgataaaat tgaataatta
    26341 aagcaattat aatcattacc atagtcatag ctacagaaag aacatgagtg taaattaata
    26401 aagtcatgct aatcgccaac ataacccaag attctttttt tgagtcgtaa agctttataa
    26461 acccgagtaa aataagagga agtattgaca ttgcgagtaa ttcccccatt gcccctcgaa
    26521 gaaatatttc tacactccta aaagaagatg ttgtatatat aatagcgaaa aaggagctag
    26581 taacatgttt tttggtaatt tcatacatta cataatgaca tataaataga gtaatgagcg
    26641 taacaatata aagaaaaagg ttatagccta ttgctaaatt agtaaacttg atcaataaaa
    26701 ataaaggata tagagttaac catgggtaaa aattatttac ttgcatgcca tatgatctaa
    26761 acgtttcaaa attaataggg gagctaaaaa tagattctaa acttaacgca cgattaatgt
    26821 gaaatgccgt atcataaaag ccaaatattt ccatgttttt aaa
//
LOCUS       DAFCHQ010000031        26580 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000031 DAFCHQ010000000
VERSION     DAFCHQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.031"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..516
                     /locus_tag="LNC71_001368"
     CDS             172..516
                     /locus_tag="LNC71_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBK6466446.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="LNC71_001369"
                     /pseudo
     CDS             517..936
                     /locus_tag="LNC71_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="LNC71_001370"
     CDS             complement(1028..1378)
                     /locus_tag="LNC71_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466447.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="LNC71_001371"
     CDS             complement(1344..2675)
                     /locus_tag="LNC71_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBK6466448.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="LNC71_001372"
     CDS             2931..3497
                     /locus_tag="LNC71_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6466449.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="LNC71_001373"
     tRNA            3642..3729
                     /locus_tag="LNC71_001373"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="LNC71_001374"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="LNC71_001374"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK6466450.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="LNC71_001375"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="LNC71_001375"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBK6466451.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="LNC71_001376"
     CDS             complement(7452..7898)
                     /locus_tag="LNC71_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK6466452.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="LNC71_001377"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="LNC71_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK6466453.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="LNC71_001378"
     CDS             8363..8881
                     /locus_tag="LNC71_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBK6466454.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="LNC71_001379"
     CDS             complement(8958..9914)
                     /locus_tag="LNC71_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6466455.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="LNC71_001380"
     CDS             10080..10886
                     /locus_tag="LNC71_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466456.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="LNC71_001381"
     CDS             10883..11872
                     /locus_tag="LNC71_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK6466457.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="LNC71_001382"
     CDS             11869..12873
                     /locus_tag="LNC71_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBK6466458.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="LNC71_001383"
     CDS             13005..13547
                     /locus_tag="LNC71_001383"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBK6466459.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="LNC71_001384"
     CDS             13567..14265
                     /locus_tag="LNC71_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBK6466460.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="LNC71_001385"
     CDS             14286..14498
                     /locus_tag="LNC71_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBK6466461.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="LNC71_001386"
     CDS             14498..15460
                     /locus_tag="LNC71_001386"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBK6466462.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="LNC71_001387"
     CDS             15478..15873
                     /locus_tag="LNC71_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBK6466463.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="LNC71_001388"
     CDS             16036..16401
                     /locus_tag="LNC71_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBK6466464.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="LNC71_001389"
     CDS             16398..18209
                     /locus_tag="LNC71_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBK6466465.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="LNC71_001390"
     CDS             complement(18271..18438)
                     /locus_tag="LNC71_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBK6466466.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18686..19675
                     /gene="galE"
                     /locus_tag="LNC71_001391"
     CDS             18686..19675
                     /gene="galE"
                     /locus_tag="LNC71_001391"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBK6466467.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19687..21171
                     /gene="galT"
                     /locus_tag="LNC71_001392"
     CDS             19687..21171
                     /gene="galT"
                     /locus_tag="LNC71_001392"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBK6466468.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21195..22175
                     /locus_tag="LNC71_001393"
     CDS             21195..22175
                     /locus_tag="LNC71_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6466469.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22366..23088
                     /gene="tsaB"
                     /locus_tag="LNC71_001394"
     CDS             22366..23088
                     /gene="tsaB"
                     /locus_tag="LNC71_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBK6466470.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            23072..23614
                     /gene="rimI"
                     /locus_tag="LNC71_001395"
     CDS             23072..23614
                     /gene="rimI"
                     /locus_tag="LNC71_001395"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK6466471.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23621..24085
                     /gene="rimI"
                     /locus_tag="LNC71_001396"
     CDS             23621..24085
                     /gene="rimI"
                     /locus_tag="LNC71_001396"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBK6466472.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24082..25098
                     /gene="tsaD"
                     /locus_tag="LNC71_001397"
     CDS             24082..25098
                     /gene="tsaD"
                     /locus_tag="LNC71_001397"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBK6466473.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(25707..26408)
                     /locus_tag="LNC71_001398"
     CDS             complement(25707..26408)
                     /locus_tag="LNC71_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBK6466474.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8601 a         4543 c         5538 g         7898 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat tttactttgt
    18601 ttgttttcac cataaaagta aacttcgttt tttaaattat atagaatcaa caaacacaat
    18661 tttactaatc aaaggagtgt tttttatgac aattttagta ctaggcggag ccggttatat
    18721 cggttcgcac gctgtagacc agctagttca aaaggggtat caagtagctg tggtagataa
    18781 tttgctcaca ggtcacaaac aagcagtaca tccagatgct catttttatg aaggagacat
    18841 ccgggataaa gaatttctac gttccgtatt tgaaaaggaa ccgattgaag gagtaatcca
    18901 ttttgctgca agctctttag taggagaatc tgtcgaaaaa ccattgatgt atttcaacaa
    18961 caacgtatac ggtatgcaaa tcttacttga agtcatgcac gagttcaacg tgaataaaat
    19021 cgtattctct tctactgcag caacttatgg agagccgaaa gagtctccaa tcacagaaga
    19081 tacaccagct aatcctaaaa atccgtatgg tgaaagtaaa ttgatgatgg aaaaaatgat
    19141 gaagtggtgt gaccaagcat atggcatgcg ctatgtcgct ttgcgctact tcaatgtagc
    19201 cggagcaaaa gcagatgcat ctatcggaga agatcataca ccagaaacgc atcttgtccc
    19261 aatcatttta caagtagctc ttggtcaaag aaaagccttg gctgtttatg gagacgatta
    19321 tgatacacca gatggaacat gtatcagaga ttatgttcaa gtagaagatt tgattgctgc
    19381 acatatttta gctttggaat acttgaaaga aggaaatgaa agcaacttct tcaatttagg
    19441 cagcagcaaa ggatactcag tcaaggaaat gctagaagca gctagagaag tgacaggaaa
    19501 agaaattcca gcagaaatag caccaagaag agctggagat cctagccgat tagtcgcttc
    19561 aagtgaaaaa gcgagagaga ttctgggctg gaaacctgaa tacacagata tcaaagcgat
    19621 tatcaaaaca gcatgggatt ggcatgtcag ccatccaaat ggttatgaag aataggggaa
    19681 tgagatatgt caatcagtca aacgatttgt gattttgcca cacttgcgat cgaagctggc
    19741 ggatggatgg aaatggaccg cttatattta cagaatcgtc ttttagggat ggtaggagaa
    19801 gatgctctag aaagcattga agtaagacca gttaaggaag aatctgtaga cttattggat
    19861 cagcttgtcg ctaaggcaag agaaaatggt gttctttcag aattgccagc agaacaagag
    19921 atttttgaag cgcaattaat ggattttctc acaccgccac cttcagtcgt caatgcattc
    19981 tttgcacagc actattcgaa aagcccagag gaagcaactg agtatttctt taatttaagt
    20041 aagaaaaatg actacatcaa aacaagagcc atcgcaaaaa acatcaaatt tccggcagag
    20101 tcggagtatg gtgaactgga aatcacaatc aatttatcta agccagaaaa agatccaaaa
    20161 caaatcgcag cagaacgcga ggcggtaaaa gtagactatc ctacctgcat gctttgcatg
    20221 gaaaacgaag gatatagagg tcgcttgaac tatccagccc gcaccaatca ccggattatt
    20281 cgaatgaatc tagacggtga aagctgggga ttccaatatt ccccttatgc ttattacaat
    20341 gaacattcga tcattttatc agaacaacac cgcccgatga aaatcacaaa ggaaacattt
    20401 gctagactgc ttcggattac tgaagtcttg cctcattact ttgttggttc gaatgctgac
    20461 ttaccaattg ttggaggatc gattctttct catgatcatt atcaggcagg acgtcatgaa
    20521 tttcctatgg caaaagcacc gatagaaaaa cttatcaaac tagaagatta tccgcaagtc
    20581 atcgcaggag tcgtgaaatg gccgatgtct gtgatccgtt tgcaatcaac agagaaaaac
    20641 ttgcttattg aagcagctaa ctcagtattg gaaaaatgga aagcatattc ggatgaaaca
    20701 gtatctatca gagctttttc agcagatggt acaccacacc atacgattac gccaattgca
    20761 cgaagaaggg acaattactt tgagttggac cttgtccttc gagacaacaa tgtttcagaa
    20821 gaacatcctg atgggatttt tcatccacat aaagatgtcc aacatatcaa aaaggagaac
    20881 attgggttga tcgaagtaat ggggcttgct gttttaccac ctcgtttgaa accagaacta
    20941 gtagaagtag agcatttctt attaggagaa gaaaaccaga tggctgaata tcatcgtccg
    21001 tgggcagaac aattaaaaaa agaacatcct gatttgacga aagatacggt caccgatgtc
    21061 gtacaaaaat ctgtaggtca ggtttttgca cgtgtactgg aagacgctgg agtattcaag
    21121 cgagatgaaa aaggacaggc agcgtttgaa cgatttactg cgattttata aacgtaaaaa
    21181 aggaggagag acaaatggca acaattaaag acattgcgca gttggcagga gtttctcctg
    21241 ctactgtttc aagagttctc aactatgatc ctgatctttc cgttggctta gccacaaagc
    21301 aaaaaatttt tgaagcagca gaacgcttaa attatacgaa acatcgtaaa aatgcagcaa
    21361 atactaagca aacgcttcgc ctgatccagt ggtacgacga tacggaagag cttgaagatc
    21421 tttattattt atctattcgc ctaggaatcg aaaaaaaggc agaagaagca aacgttactt
    21481 tattgaaaga aacgtgggat tccattagtg atcttccggc agatggaacg atcgcactag
    21541 gaaaattcga cgaagctcag ttattagaaa tgaaaggatc ccaaaatgcc ttgcttttgg
    21601 tagattctga cggcacttct caaggaatcg actctctagt tgtcgacttt aagtcaagtg
    21661 ttcaaaaagt cattgaacac tttgtctccc ataaagcgca acatatcgct atgcttgctg
    21721 gaacagaata tacgaagaaa aaccatcagc ggctaaaaga tcccagaatt atggcatttc
    21781 aacaagtttt ggcagaaaaa ggctacgacg aaggtccaat tatcgaagca gatttttctg
    21841 tcgagtcagg atatcaagca gtgaagcagt atttaaaaac gaatccgcat gttcctgatg
    21901 cgctttttgc agctaatgat gcattagcga ttggcgcact gaaagcgatt caagaaaaag
    21961 gtttagtggt accagaagat atttctgtga tgggcttcaa tgatgtcagt gtagcgaaat
    22021 atgtaacgcc ctcgctttct actgtaaaag tttataccga atggatggga gagctagcag
    22081 tggagacgat tcttcaatta agtaattcca aagcaccggt ttcaagaaag attattgtgg
    22141 aaacagagtt gattattcga gaatcaacga aataaaagat ggaaaagctg tgggaaaatg
    22201 gagtgatacc gcagcttttt tgtgtttcag gaaggggcaa aatttgcccg ccagcagata
    22261 atcatgtatt attgtactag ttttgccttt ggtattttca tcctcagaat aaaaaagtat
    22321 gctacaatgg aaggcgagaa tgtcatagca gaaaggatca aatatatgat tacattagga
    22381 atagatactg caaaccaaac tttggcgatc ggtgtggtag aagacgaaca aattcttgga
    22441 caaatacaaa caaatatcaa acgcaaccac agtgtaacac tgatgcctgc aatcgaccaa
    22501 ttgtttgcag atcttcaaat aagtccaaaa gacattgacc gaatcgcggt ttctgatggt
    22561 cccggttctt atacagggct tcgtataggt gtaacgacag caaaaacgat tgcttataca
    22621 ttagataaag agctagtagg agtatctagt ctgaaaacca tcgcggctaa ttgcatcggt
    22681 gtggataata ttattgtgcc catgtttgat gctcgaagaa aaaatgtata cgctggggca
    22741 taccgctgga aagaaaatag attagaaact gtacttgaag atgtacatat ttctgcttct
    22801 gatttgttca cacaactaca aaagctgaat gaagctattt atttcgtcgg gagtgattgt
    22861 cacaaattct tctctgaaat aaaggaaata ttgcctagtg cgatgattaa tacagttcca
    22921 gcatgggata ttccaagtgg tgtaactgtg gctcaattag gtgctttagt tgaaccagtt
    22981 gcagagaaac aagcttttct tcctcgctat ttaaaacgag tagaagcaga agaaaaatgg
    23041 ttggagacgc acacacctgg agacgaaagt tatgttgaaa aaatttagag cgtatgccag
    23101 aagctttttt caagataaaa tgatttatac agaaaaaaca gtcgaacaaa tcgatcgtcg
    23161 ttacgtacta agagaattga ctacagaaga tatcaaagag ctgctggatg tcgaacgaga
    23221 agtctataat ggcgaattgc catggacaaa aagtgccttt atatcagaat tgaagtcacc
    23281 gcttcctcat ctatatctag gtatctttga taatggaaga atgattggtt ttatcggttc
    23341 aagagcgttc ggtttagatt gccatatcac gaatattgcc gttcgaccgg catatcagcg
    23401 taaacgaatc ggttcgctgc ttattgacga aatcgagaac tttgctataa tgaatcgctg
    23461 tgagacgatg tcattagaag tgcgtatgag taatcaagat gcacaaagac tttatcggaa
    23521 attggggttt caagcaacaa cgatcaaaaa aagatattac actgaaacaa aagaagatgc
    23581 gttggatatg ataaaacttt tagaaaatga gtgagattag ttgatttgca gaaaaaaaga
    23641 ttttgatgaa gcgtacttgg cagaatgcgt atggatgatt agcgaaaatg cttatccaca
    23701 tggttcacct tggacgaaaa tgcagttttt ggcagacatc caacagcctc actctgatta
    23761 tctgctttta gctgaagata accagatctg tggctttatt ggatactcga aagtaataga
    23821 tgaagtcgag atcacaaata ttgctgttgc agtttcagaa caaggaaaag ggcatgcacg
    23881 gcatttgtta caattactga ttacggaaca aataaaagat tcgctgcatg tttttttaga
    23941 agtacgtcta tctaatgaag cagcaagaaa actttatgaa tcagaaaagt ttcgtatatt
    24001 aggtaaacgg aaatcatatt atcgcaatcc gacggaagat gcgatcatta tgagcagaaa
    24061 gttaaaaacg gagacgatga aatgaatgaa gaattgattc tggcgattga atcaagttgt
    24121 gatgaaacga gtgtagcggt cgttagaaat ggaacagaga tcttatctaa catcgttgct
    24181 tctcaaataa acagtcataa acgttttggt ggcgtagtac ctgaagtagc tagccgccat
    24241 cacgtagaac aaatcacctt atgcttggaa gatgcactgg tagaagctgg tgtgtcggca
    24301 gaagatttat cggcagtcgc tgtcacttat ggaccaggac ttgtcggctc gttgctgatt
    24361 ggtatttctg cagctaaggc ttttgcttgg gcacaccaat tgccgcttat tcctgtaaac
    24421 catatggcag ggcatatcta tgcagcccgt ctggttaaac cattccagtt tccattgatg
    24481 gcacttcttg ttagcggagg acatacagaa ttagtctata tgcaagaaga tggatcatat
    24541 gaaatcattg gggagacaag agatgacgca gcaggagaag cttatgacaa agttggaaga
    24601 gtattaggtc tttcttaccc aagcggaaaa gaaatcgatc agctagctca tcagggaaaa
    24661 gataattacc attttccgcg agcgatgatc catgaagata attatgattt cagttttagc
    24721 ggattaaaaa gtgcatttat caatttggtg cataatgcac aacagcgtgg agaagatcta
    24781 gacaaaaatg atttagcagc tagctttcaa gcaagtgtga tcgatgtatt gattactaaa
    24841 acacttcgag cttgtcaaaa ttatccagtc aagcaactga ttatagctgg aggtgtagcg
    24901 gccaatcaag ggttgcgtga aggattgcag gatgctttgg caactaaact ccctgaggta
    24961 gaactagtga ttccccctct tcgactttgt ggagataatg ctgcaatgat tggagcagca
    25021 gcacatgtgg aaatgcaaaa aagaaatttt gctagctatc agttgaatgc tgatccaagc
    25081 ttggttttat ctgaataaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    25141 gcccaggaaa aacggcttct catgtttttg acctggctgt tcttattacc gcgtatgcaa
    25201 gcttttgaac gccgtttaat caagagttcg taagcctgtc gtttaactct gattcacaag
    25261 gaacaatttc aaaaaacagc tcctcatgtt tatgaacatt atgacttgtg accgaagagt
    25321 tgacaggtac acaccgttta atcgaaatag ggctgtgaca cgtttttgtc aaagccctat
    25381 tttttacata cttttaatca ttattgttat tttgctattt tttacagctt atttatcatt
    25441 atccgttcct ctgattgccc tcactgagta atcgtttatt gtcgaggaat atcgaataat
    25501 ctagatcgga tgaggacaag acgcttcatc tagttaatct aacagtattt tttgcaaaca
    25561 gtgaatatta cataactgaa acacacatag aaaatctttg agtcttgtcc agtgatctaa
    25621 aactgccgga tctttcagaa gttttaggga ggaaaataag cagtgaccct gatatttatc
    25681 agggtcactg cttatttttg ttcgcgttat tctctcatag ctggagtagt agctgtctta
    25741 cagaaagtaa aacgcttttt gtcataatct aacagcccgg aatcacgtag ttgtttgatt
    25801 acttgtccag cagtttcacg tgtcgtgccg ctattcactg ctaattcttt taaggtgatc
    25861 ggataaggaa tggtgatatg accatctttt gttggtacgc ccaattcttc tttcaaaatg
    25921 aataaatttt gttttacccg gtcaaaggcg ctagaggtca atccgatttg gatacgcaat
    25981 tcatgtattt caactacttt agaaattttt tgtatcatat agatcaactg ttcgggattt
    26041 tttcgtgcta atttttcata gtaggaaaca ggaaggtaga atgtttcaat atcagtcatc
    26101 gcttgagctg tataagtgta ggtctcttct ccaaacattc ctgacatagg aaaaaggtta
    26161 tttgtattca catagtcata gtagtaataa gttgcacttt catcataacg ttctaaacga
    26221 acgagtcctt tggtcaagat atacaaccgt tgtctgggat caccatcgtc gaaaaggatc
    26281 tgtccttttt tataagaacg aagaacagta tgttccttga tctgttggaa ctcttcatcc
    26341 gagaaatgtt caaaatcagg ttgatatctg agttttgaaa agtgaaaatc gattttatta
    26401 acacgcatta aaaagtcctc tccttattgt tcactatatt tattgtcact ttttttaaag
    26461 aatttgtaaa gttttaacat accaacatta gaaacaagtt ttaatacttt acaaatataa
    26521 tttgttatta tgtaaaaaat atacaatggt attgaataat ttgttttgaa gcgaatgatt
//
LOCUS       DAFCHQ010000032        26054 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000032 DAFCHQ010000000
VERSION     DAFCHQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.032"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..256)
                     /locus_tag="LNC71_001399"
     CDS             complement(<1..256)
                     /locus_tag="LNC71_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBK6466475.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQ"
     gene            complement(347..799)
                     /locus_tag="LNC71_001400"
     CDS             complement(347..799)
                     /locus_tag="LNC71_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK6466476.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(812..1222)
                     /locus_tag="LNC71_001401"
     CDS             complement(812..1222)
                     /locus_tag="LNC71_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6466477.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1497..1709
                     /locus_tag="LNC71_001402"
     CDS             1497..1709
                     /locus_tag="LNC71_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6466478.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1712..1924
                     /locus_tag="LNC71_001403"
     CDS             1712..1924
                     /locus_tag="LNC71_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466479.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2064..2219
                     /locus_tag="LNC71_001404"
     CDS             2064..2219
                     /locus_tag="LNC71_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466480.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2216..2440)
                     /locus_tag="LNC71_001405"
     CDS             complement(2216..2440)
                     /locus_tag="LNC71_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBK6466481.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2518..2706
                     /locus_tag="LNC71_001406"
     CDS             2518..2706
                     /locus_tag="LNC71_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466482.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2703..2876
                     /locus_tag="LNC71_001407"
     CDS             2703..2876
                     /locus_tag="LNC71_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466483.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            3032..3367
                     /locus_tag="LNC71_001408"
     CDS             3032..3367
                     /locus_tag="LNC71_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466484.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3600..4541
                     /locus_tag="LNC71_001409"
     CDS             3600..4541
                     /locus_tag="LNC71_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBK6466485.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4543..5433
                     /locus_tag="LNC71_001410"
     CDS             4543..5433
                     /locus_tag="LNC71_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBK6466486.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5476..6276
                     /locus_tag="LNC71_001411"
     CDS             5476..6276
                     /locus_tag="LNC71_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK6466487.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6291..7142
                     /locus_tag="LNC71_001412"
     CDS             6291..7142
                     /locus_tag="LNC71_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6466488.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7343..7804
                     /locus_tag="LNC71_001413"
     CDS             7343..7804
                     /locus_tag="LNC71_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6466489.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7830..8282
                     /locus_tag="LNC71_001414"
     CDS             7830..8282
                     /locus_tag="LNC71_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466490.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8388..8582
                     /locus_tag="LNC71_001415"
     CDS             8388..8582
                     /locus_tag="LNC71_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466491.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8613..8906
                     /locus_tag="LNC71_001416"
     CDS             8613..8906
                     /locus_tag="LNC71_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466492.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8936..9376
                     /locus_tag="LNC71_001417"
     CDS             8936..9376
                     /locus_tag="LNC71_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6466493.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            9451..9918
                     /locus_tag="LNC71_001418"
     CDS             9451..9918
                     /locus_tag="LNC71_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBK6466494.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10665..10895
                     /locus_tag="LNC71_001419"
     CDS             10665..10895
                     /locus_tag="LNC71_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466495.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            11054..11887
                     /locus_tag="LNC71_001420"
     CDS             11054..11887
                     /locus_tag="LNC71_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBK6466496.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11880..13295
                     /locus_tag="LNC71_001421"
     CDS             11880..13295
                     /locus_tag="LNC71_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK6466497.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13307..14836
                     /locus_tag="LNC71_001422"
     CDS             13307..14836
                     /locus_tag="LNC71_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK6466498.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14754..15806
                     /locus_tag="LNC71_001423"
     CDS             14754..15806
                     /locus_tag="LNC71_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBK6466499.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15808..16125
                     /locus_tag="LNC71_001424"
     CDS             15808..16125
                     /locus_tag="LNC71_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466500.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16252..16875
                     /locus_tag="LNC71_001425"
     CDS             16252..16875
                     /locus_tag="LNC71_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBK6466501.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16890..17780
                     /locus_tag="LNC71_001426"
     CDS             16890..17780
                     /locus_tag="LNC71_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBK6466502.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17803..18018
                     /locus_tag="LNC71_001427"
     CDS             17803..18018
                     /locus_tag="LNC71_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466503.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18030..18371
                     /locus_tag="LNC71_001428"
     CDS             18030..18371
                     /locus_tag="LNC71_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466504.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18371..18742
                     /locus_tag="LNC71_001429"
     CDS             18371..18742
                     /locus_tag="LNC71_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466505.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18735..19130
                     /locus_tag="LNC71_001430"
     CDS             18735..19130
                     /locus_tag="LNC71_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBK6466506.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19132..19509
                     /locus_tag="LNC71_001431"
     CDS             19132..19509
                     /locus_tag="LNC71_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466507.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19510..20118
                     /locus_tag="LNC71_001432"
     CDS             19510..20118
                     /locus_tag="LNC71_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6466508.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20118..20459
                     /locus_tag="LNC71_001433"
     CDS             20118..20459
                     /locus_tag="LNC71_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466509.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20701..23490
                     /locus_tag="LNC71_001434"
     CDS             20701..23490
                     /locus_tag="LNC71_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBK6466510.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23480..24220
                     /locus_tag="LNC71_001435"
     CDS             23480..24220
                     /locus_tag="LNC71_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6466511.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24217..>26054
                     /locus_tag="LNC71_001436"
     CDS             24217..>26054
                     /locus_tag="LNC71_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6466512.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9395 a         3731 c         5277 g         7651 t
ORIGIN      
        1 tttgatttat ttcatcattt aaatcttctt tctctttttt taataaaata atatcatttt
       61 ttaacatagt aatttgttta tttaaatctg atagtgtttc ttccttactt aatataaatc
      121 tttctctatc atgtaattta tttagttttt catcattttt ttgttctaat tcataagctt
      181 ctttttttat tgtgttagct ttcgacagtt tagtgattcc tataattaat aaaataagtc
      241 caatcaataa taacattcaa aactcctcat ttctttgata taattattgt agagtttact
      301 cagaaatgag gaaagagtcc atgtgaaagc atgggctttt ttatttttaa tgttgtaata
      361 ctaaatgttc aagattatga ggaagcccta aatattcaat aatttggaac tgactcatat
      421 cagataaatc ttgttcatct attgttctag ttaacatttc aattgcaaac atattagctt
      481 cagcttctat accataaatc atacaactaa ggttgtttgc tctaaaagct gctgtgcaaa
      541 tacctttatg gagtctaacg tgggctattt catgccataa tacaaatctt ttcatagaag
      601 gtgataggtt ttgattaatt aatatcacat aacatctttt attgtgtatc gtttgtccta
      661 atgttttatt ccctaaatca gtttcaatta aatctatatc agaatatttg attattttat
      721 atggattaat cgcatcagat ccaaactcta caaataattt ttctatttct tctaatatcc
      781 agtatttgtt taaatgcata ccaaagccca cctattgacg atatttttta ggagtgaatt
      841 ttttctttgc tttaactttg gttaaggcaa gtgcaagttg aatggcatcg tataattttt
      901 ccttatcttc atcgtctaaa ggttcaccat aataatttgt ttcagcatct gaattcaagc
      961 cttcaagtaa tttttcagct tgtaaagcaa tatcttgttt atctttttct gttaaatcat
     1021 aataatggcg tttatcagta cgtcctaaaa gataatcagt tgatacatta aagtaatctg
     1081 caactttttg tattttatct ccagatggag tacttttatt ccatctagac atgcttccat
     1141 ttccgaaatt taactttctt tcaagttctg ccaacgtcat tgaacgttct tttaataaga
     1201 tttttatacg gtctaatagc ataggatatg cattcctttc aaaataccga caaaaaaagt
     1261 atttttctgc aaattatcta tttacaaata gagaatttgc agttatacta gttttgtaag
     1321 caagatattt acggcataca aagactaaaa gcaataaact aatagtctcc ccagacatca
     1381 gtaagctcgc tttttgtgcg ctgtattttg tatgcttaca taatagcata tttgcagttt
     1441 ttgtcaaact tattagacaa aaatattaga aaatattcga taagggggta tgaagcatga
     1501 ctttaaaaca gcaagtatta attgctttag taaagaaagg ttggagtcaa agagagttag
     1561 ctcgacgaat gggtatatca attacttatt tacgagatat cttcatcgaa aaaagaaaac
     1621 ctaaggaacg tttaaaacaa atcgaagaac ttttggatat caagttagaa gtagattcta
     1681 atgatcagcc atcagaacag gaggcgtaaa aatggtacaa cgtttgagca aagcggagtt
     1741 taaagagaaa tataagtatt ctgaaagtac ttatcaaaga agaatgaagg aatttaaaaa
     1801 atcacgtttt agtgaggggt ataacgcagt tacttccaaa gaaataacaa ttgatgtcga
     1861 tttatatgaa cagtttagaa catggcgttc taaaaatcga tttagagtta gagctgtttg
     1921 ttaatagcta aaaaatctaa attcaccact tctgaaaaga ggagttgctg ctctcctttc
     1981 aagtttgcca aagtccacgt tttttcattt tggcaactcc tcttcttgga ggtggtgacg
     2041 gcgagagacg aaggagggat gacatgaagg aagaatcaaa aatagggatt ttgaatgaga
     2101 acaattacac aagattaatt attgaaacag aagaagggaa aaaagtagca gaagttacat
     2161 tgaccgatgc aactcctgct gacggttatc gtattcgatt aactcctaaa tatgattaac
     2221 cttttggtgg gtgtggatcg tttccgtgac tatcacggct ttgaattttt ccatctctgc
     2281 catgaataaa tagctcagat ccttgatttt tagaaatctc tctagcgatt tttactgctt
     2341 ctgattttgt attagtatgt gcagtcgctt tagaatttcc agcacctttg acattccaac
     2401 caccattgga attaggtaca acatgttgat tttttcccat agttttcacc tccatttcta
     2461 tagaaatatt ttatcaaata gaaatggaga aattcaataa acgaaaggag aagtgatatg
     2521 gcatatacct tgcaacaaga acatcaaatt ctcggtttga ttaaacaacg caggaaacaa
     2581 ttacaagatg accgtgcagc gcttagaaaa gccgatgagc tatcagatag gcaagctgaa
     2641 ctaattgctt ctgaacttga ggatttgaga atgctagaaa taaaaaatag ggagattaga
     2701 ttatgaagaa gacagacaca ctttttatag gattcatttt ggggttatta gtgattgtag
     2761 cgcaccaaag tattatcggg ggaagcttgt tcgcagcatt gatggtttta atcaatctgc
     2821 ttgattcaaa agaaaggagc aactatggca cgagaagaag cgctaaaaat cggtaaagtg
     2881 attgctgata atcggtgggg aaatagccgc cctattattt taagcaagca acatatcgaa
     2941 aagcaaaaag catggcaaca aataaaaaag tgactctgcc agccagcaaa gagtcacata
     3001 caaatttata aaactaggag aattatatca catgaataag aaaattgaaa atcttatcga
     3061 agaattgaaa cgtgaatgcc aaaagcaagg tgtttctatt atttgtacag cccaaaaaga
     3121 aggggaactt aaaagtctcg tttatggtga gacaacagaa attttacttt gtcttgctat
     3181 gcaagaagaa catttagatg aaaatctccc attgtcagca catattatgc gtagaattgc
     3241 tgtggatgct tacgaacaag cgaaaaatga agaagaaaat caaccttcaa atcatacttt
     3301 tgttattaat aacaaggaag atttagctga tgtgatgact cgtattctca agggggaatt
     3361 tcaatgatga atgaaaaaac aattgatcta ctcattattt tatttttcac gttagtgatt
     3421 gtagcgaaaa gttatgtaat ctttggcttg ttattcgcaa ttaccatgct tgtgctttgc
     3481 gtgttagcaa gcagaaaaga ggttaaacat gaacgagtta gcaaatcttg acaactattt
     3541 aactgatcct gattatgcta aaccaccata tgaagcacca attgatgagg aggatgaaga
     3601 tgagtaaatc taccttagaa atgagccatc aagaatggct tgaagaccgt aagaaaggca
     3661 tcggaggttc ggatgttgga acaattttag gattgaataa atggaaatct ccttatcaac
     3721 tatggctaga aaaaacagga caagtagtac ttgaagaatc aggaagtgag cccgcttatt
     3781 ggggtaatgt tttagaagaa gtggttgcta aagagtttca ggaacgtaca ggcaaaaaag
     3841 tacgcagaag aaaccaagta tttgaacatc cattacatcc atttttaaga gcaaatattg
     3901 atcgggaagt agtgggagaa aatgccattc tggaatgcaa aacagccaat caatttctcg
     3961 gtaaagaatg ggaaggcgaa gaggtaccgc ttagctatct ctgtcaagtt caacattaca
     4021 tgaacgttct aaacaaagac tattgttaca tcgctgtctt aatcggtggt caaaaattta
     4081 tctggaagcg gattgaacga gatcaagagc tgatcgatac aatcactgaa caattagtag
     4141 agttttggga aacaaacgtt cttggaggta tcgagccaat tattgatggt agtcaagcga
     4201 cagctgattt cttgaaagag aagtatgcag atgttgaaga cgttcaaacg acgttaccaa
     4261 ttcatttcga tgaactagtt gaacagaaaa acgaactcaa acggactaag aaagaaattg
     4321 aatcagctat ccgtcaagta gacaacgaga ttatcagcga gctaggtaaa cgtgaagcca
     4381 gtatcggtat cactcaaaaa aacatcatca gttggaaatt tgtccgtacg agacgtatga
     4441 actcgaagaa actagcagag aaatatccag atgtcgcaaa tgatgaagag atttataacg
     4501 ttactgaatc aagaaggcta accgaaaggg agatcaaata atatggcaac aaatgaatcg
     4561 ttaaaaaatc aattggcagc aaagccacag aaacaagttg caccaggtca attaggactt
     4621 aaagctttga tgaatacacc aacaatgaga aagaagttcg aagaggtgct tcatgacaat
     4681 gctaatgctt ttatgtcgaa tgttatgact cttgtatcta atgacagtta tcttgcagat
     4741 agtgaaccga tgtctatcat gagtggtgcg ttaactgctg caacattaaa tcttgggcta
     4801 gataagaatt taggttatgc atatttagtt ccattcaata gtaaaaacaa gcaaacagga
     4861 aaatgggaaa agaaagctca attcatgctt ggctataaag gatatatcca attagcccaa
     4921 cgatcaggta aatacaaagc attaaatgtg atcgaagttt acgaaggaga actaaaaagc
     4981 tggaaccgac tgacagaaga gtttgagttt gatccaaatg gtagaacgtc tgatgaagtc
     5041 attggatatg ttggctattt cgagttactg aatggattca aaaaaactgt ctattggacc
     5101 aaacaagaaa tcgaagctca tcggattgct aacaataaag atcgcgataa gacaaagtta
     5161 agtggtgtgt gggcatctga ttacaatgca atggcacgaa aaactgtttt gagaaatctt
     5221 ctttctaaat ggggaatctt gtccatcgaa atgcaagaag ccaccacatc ggatgagaga
     5281 gtccaaaggg ttcaagaaga cggcagcatt attgctgaaa cagaagttga agaagatatt
     5341 cctgaaagaa aagaagcaga ggttatatct gaagaaaatg aagatgtaca aactggatta
     5401 tttgatgcat ctaatccgcc gttaaacaaa taatgaggga gctttctccc ttgcttttct
     5461 agaggagaaa tacgaatgaa cacaggatat gtcaaattac atcgaaaagt gatgaattca
     5521 ttcgtttgga ccaatcctta tatgtataaa ttatggaact tgtgtttgat gaaagcgagt
     5581 catgagaatc gcaagatact ttttaatgga aaagagatac aagtgaacag cggagaattc
     5641 gtcacagggc gcgatgctat cacatctgag atgaacgaag gcgtgaaacg tgaacatcaa
     5701 gtgaacagcg gttctgtatg gagatggcta aaacaatttg aaaaaaacgg aatgttgaac
     5761 atcaaatcaa ctacgaaata cagcgttata tcaataaaaa actggtcttt gtaccaaagc
     5821 agtgaacaac aaatgaacat caaacgcaca acaagtgaac aacaagtgca cacaaacaag
     5881 aatgaaaaga attataagaa tgaaaagaat aataaaacat catcgtcgca accacgcaaa
     5941 aagcgtgttt acgacaccga ctcagtttac tacattctcg cggaggagtt attcaaacag
     6001 atttgtcaga atcaggaaat caaaaagccg aatctgcaaa gttgggcaga tgacattcgg
     6061 aaaatgatcg agatcgacaa gcggaccgaa aaccaggtac ggggaatgat tgaatggagt
     6121 cagcacaatg tgttttgggc atcgaatatt ttatctgcta aaaaattgcg agaaaaatac
     6181 gacacaatgg cagcgcaagc aaatcgtgat tataaaacaa aacaaactaa aacgcttgaa
     6241 tacgagaaat ttagcacaga tgagttgcct atttgagagg aggcgtaggc atggaaactg
     6301 ttggggaaat catggaaaag ctgatacaga aagtgcttgt ccaacgtggc gaatgtcctg
     6361 aatgcggaca gcctttgtat ggatggcgta cgaagaaccc tgatggctca gaacgttgta
     6421 agccaacctg tatgcaatgt gggtataaag cattgcgagt tcaggaagac ttacaaacag
     6481 aacgaattta caacgagagc ctgaaagcaa gagcaatcaa ttttttcaaa ggtggttctg
     6541 ttgtgcctaa tcaagcgttg tttgattgca cattgcagaa ttatcaaatt gtcgatcaag
     6601 aaacaagaca agcggttgaa gtaaccaaac gctttgttaa ttcagtcttg ttaggaaatc
     6661 caagtcacct tgttttaact ggaaaacaag gaacaggtaa aagtcaccta gcgatggcag
     6721 cggcttggga agtcttgaag cgatcaaact acgataaaaa aatcttattt atcgggttac
     6781 aggaaatgct ggatcaaatc aaattttcgt acaacaatcc tgaactcaga aaaacgattg
     6841 agggatcgtt gattgcagat atcaaaacag cggatttagt catcattgat gatatcggtt
     6901 cagaactagg aaaagatgca tcagatagtc gagcgtttgg cataaacacg ctaaattcgt
     6961 tcttggatgc acgacagaac ctagcaacga ttatcacaac gaacttgctt ggtgaagaac
     7021 tgaaaaaagc ttacggtacg agaacaatat caagaatgtt tgtcaactct gatggattta
     7081 cgatggtatt ttctcaaaca gcagacaagc gcataaaacc agtcaaagga gcgatcgcat
     7141 gaatttgact aaacaagaaa aaagaatctg tcgtcagata atgttgcaaa ttataggtga
     7201 tcgtgacatg gacgaattat ctcaacaaga gagtcgaatg gttcggaaga taagtaagct
     7261 aattatttat cgggatacag aaagatttct cactgtttta ggtaacgtct acgagaatcc
     7321 agagttattg gaggtagagt aattgccaaa aatattagat gcatgttgcg gtagtagatt
     7381 gttctggttt gataagaaca attctgacgt aacatttatg gattgtcgtc agcagtatga
     7441 agaattatca acaggtcatg tgatcaatgt tgatccagat gtggtcgcag actttcgcga
     7501 tatgcctttt gaagatgatg tgttcgatat ggttgtattt gacccgccac acttaagaca
     7561 tgtcggcgaa aactcatggc ttgccaaaaa atatggaaaa ttagacgaac tttggccaga
     7621 agacatcagg caaggttttg cagagtgtat gcgagtttta cgaccttcag gaacattaat
     7681 ttttaagtgg aacgaggaac agataccact ttctgatgtg ttagaggcta ttggagaaca
     7741 gccattgttt ggtaataaac gaagcaaaac acattggctt gtgtttatga aaaaattcag
     7801 ctaacggagg aaacaaagga ggaaaagtaa tgaagattgt attaaataaa tgttttggtg
     7861 gctttgggtt gagccatgaa gcaaaaatgg aaatttttaa aagaaaaaat attgaagtat
     7921 tcccatacat caataacttt agctatgact cagatgatga atacacgaga tacacaggcc
     7981 aaaagctagg ttcgatggat tttatctact atttcaaaaa agacccgaaa atcgacaaag
     8041 ttacaggaat ttatagcgaa attgaccgac tgtacggaat tgctgatgat tccagttttt
     8101 caagtgattc aaacagaggt gataaggacc tcgttgctgt tgtcgaaaaa ctaggaagtg
     8161 aggccagtgg accatatgca agtcttaaag tcgttgatat acccgatggg gctgagtggg
     8221 aaataagtga ttatgacggc gttgaaactg cacattatgg ttttcaaaca ggtagtattt
     8281 agtccactaa tgacaactaa agaggaggca gaaggatgag tgaagatgaa tactttgaac
     8341 agtagtctat taacgacaga gatttttaac tggaaagggg tgtagtcatg gacgcatcgg
     8401 aagtagtaaa taaaattaat agtctcaaag aggttttcgg agacgtcgaa gtagtgataa
     8461 acgcacaagg ctatgacaac gaatttttta agcagattac cgacattagc attcagcaag
     8521 gaatggaaga cgaagacgga aactttatcg atgaagtagc cattttagtg acttgcgagt
     8581 aagtaattga cagctaaaga ggaggaagcg gaatgaaact aaaagacgga ttttactcca
     8641 gcggccacgg catcggcggt ttaatgctag atatgccgac aaagaatcct aaaacacgta
     8701 agaaaccaaa attcaaagtc ggtgacatgg ttcgctgcga agcagaagga ttcatctatc
     8761 catttcgtgg atatgtagaa cacgtctata atcactcagc aatcattcgc attgaaaaca
     8821 cgatggaatg tgataagtgg ttagcgaaaa gcaaagagaa tttagcagtg gtgagattgg
     8881 tggacatgga agttataaaa tcttaatttt gcttttttac aactatccta caacaatgag
     8941 cgttattcca caactggaag gagaagaggc aattgtgaac attttagaga atatagatat
     9001 taagcaaaca aggaaaaatg ctagacgatt actaaaaaga tacagaaatt tagaacgttt
     9061 ggtcggacca gtgaaaatag acttttccgt gatgactgtt accaaaaatt tgaaattcac
     9121 aattgacagt caaaacgaag aaatcattga agcgataagt actcgagatt cggttatcga
     9181 agcattaaca cggctaagta gaatccattt ccaggtactt tattatagtt attgttttcc
     9241 taataagatg tcaatgtatc aaataggaga aaaattagga tattccgata gaactatcga
     9301 gagaatgaag gcagtagctt tggttgaatt tgctgaggcg tataaatcag gagaactcat
     9361 ttcacgtaca aaataaaaaa agtcggattc ctccgactat gaataatatt tccgacataa
     9421 gtattatatc ataattgggg gaatcagagg atggtacttt tcgatgtaaa gaaatatgag
     9481 acaccaagcg cgaaagatgt tgatatggaa cgcacaaaac ataatgtcgc tgtgtttctt
     9541 tcagcatatc tatcagctag atgtagagta gggcaacatc gagaaccaaa agtgacagca
     9601 tcttattcct tggttccacc ttctacagct gatcatatat ttgaagcaga aagaatgctg
     9661 atcgataaag aagaagcaca ggaagaattt gagtatctgc ataaattgtt tgttcgaggc
     9721 tattcagcga tacagcatcc gcataagcct gatgtgactg aaaggcgcaa gaagatattc
     9781 tatgatcgtt atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa taatactagc
     9841 gaagaatcgg ttaaagtaga atcaaacaga attatcatcc aatttgcttc atcgttagaa
     9901 ctggttgctt tcaagtagcc agtttttaca ctttttatac ccttttatta ccaatttggt
     9961 ttccatttta tacctttttt ataccaatca cttacctatt caacgttgta ttatgatagt
    10021 gtcgaaaaat taggaaacag gatcgacaaa ataaaatgta agggaggaaa tctccctcat
    10081 cgtttaatta agcttcgata ggcagcaacg gaaatattaa gaataaggat gtgaatttca
    10141 actccttcta aattgttctt attatctatc atccgttgct gtctattgtt tttttaatta
    10201 ttcacacgat aactaaaggt aagtgaagag aaatgattcc attaataatt tcaatttttg
    10261 cgctttgtct taatgtatat atgattggat ttaaaaatgg gcaaaataaa aaatagtagc
    10321 agccaagaat aattttatag tgtcactgtg gcggaaaggg tagacgctaa gcatgtgtgc
    10381 taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagtgact ttggcaaccg
    10441 aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg
    10501 taggttgcta ttacataact ggttaggtta gattgagatt tgggattcgg tacaaatgaa
    10561 tcgtcaaatg tctcaagcac aggatcggaa acgtccctgc ctgtgcatta catattagat
    10621 cactctttga gtggtctttt tctttacata aaggaggcta cataatgaga aactactggt
    10681 atgtatcgct aactaatgaa tatcctcgaa ccattgatga ttgttcagtg cgtgttgtgc
    10741 gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat ggggagagaa gcagaaccat
    10801 gtgagattga tgcgtgcaag ctcgtttatt gcggtcatgg tttctatgat gaaccaaaca
    10861 ttcaaaataa tattaacaag aatttgaggg attagaatgc aagaaatggc ttaaaaatct
    10921 ccaatcagga caaatcaagt tagacaaagt ttccgattta aagatattga ttgaagctga
    10981 tttaatgcta aaagatattg aaaattagaa aacaaaactc aacctaaaca gaatgtgagg
    11041 tggtgtgtat tgaatggcaa gacaacgtga tccaagacgt gatgaagcca aaagaatttg
    11101 gttagaatcc aacggagaaa agcagttaaa ggaaattgca tctgaattaa atgtttcaga
    11161 ttctcaggtt agaaaatgga aatcgcaaga caaatggagc gctgaattga ttagtaacgt
    11221 taccaatggc aaaagtaacg ttactaatca aggtggcgct cctattggta atcaaaatgc
    11281 taaaggtaac aaaggaaata gccgagcttc tccgccagtg ggtaataaaa acgctttgaa
    11341 aacaggtgag tatgaaacaa tattttttga cacgttaacc gataatgaga aggacatcta
    11401 ttctagtttg aatgatgatc cttcttttgc tttgtccgaa gaaatacgac ttcttaaaat
    11461 aagacaactt cgaatgatga aaagaatcca acaagctgaa gctggactaa atgatgaaga
    11521 agtcgaacga ttgcagcagc taagaaagat taaaactccg attgaaaaaa atggtaaaaa
    11581 gctagaaatc aagcgtgagg ttatgcaaga tgtgcagatt agcagaaaaa aacatcgcaa
    11641 aattgatgat attctttcaa ttgaagattc attgactcgg attagcaacc agttagctaa
    11701 agccatcaag caaatgaatg aactttatat gaatgaatac agaactgatt taattaaagc
    11761 tcagactgat aagatccaag ctgagacaaa tgaaattggc ggaaataatt caggtgaaga
    11821 aatagaagaa tggaaacagg cagttttaaa tgccgcaaac aaacgggcgg tgaaagaaaa
    11881 tgaataatga atttattcct tttgctgata ttggttctgc cattgattat tactatgata
    11941 aaccagtagc tttttgccaa gatattttgc atttgaatcc tgatgaatgg caagaaaatg
    12001 ttttaaatga tttagctgaa ttttcaaagg tttctgttcg ttctggtcaa ggagttggaa
    12061 aaacagcatt agaagcagga gcaatacttt ggttcttaac gtgtcgaccc tacgctaaag
    12121 taatagcaac agctccgaca atgaagcaac tttacgatgt actttgggca gaggtagcta
    12181 aatggttaaa tgatagcttg atcaaaaact tactgaagtg gacaaagacc aaaatttata
    12241 tggttggtga ttcagagcgt tggtttgcta cggctagaac agcgactaaa ccagaaagta
    12301 tgcaaggttt tcacgaggac catatgttga ttgtggtaga tgaagcttct ggtgtgtctg
    12361 atccaattat ggaagctatt cttggtacgc tatcaggttt tgataataag ctgttgatgt
    12421 gtggaaaccc caataatatt gaaggtgtct tttatgattc ccataattca gaccgtgata
    12481 aatacagagt tcataaagta tcaagctatg atagtaaacg tacaaacaaa gacaatatag
    12541 aaatgattct taaaaaatat ggaaaagaaa gtgatgttgc tcgtgtccgt atttttggag
    12601 aatttccgaa aggtgcgttg gattcattta tcagtcttga aacagttgaa ttggctacag
    12661 aaaaacaaat tagtgattct ttagtcaata aaacaaaggt tgctcatatt ggtgtcgacg
    12721 tagctcgata tggtgatgat tctacgattc tctttcctag aattgctacc agggcattgg
    12781 agtatgagaa gtattcaaaa cgtagcacca tggaaacaac aggatatgtc atcaacatgg
    12841 ccaagaatct aatgagtcaa tatccgagta ttgataaagt gatgattaaa gtcgatgaca
    12901 ctggtgtcgg aggtggtgta accgaccgcc tagaagaact tatagaagac aaacattatc
    12961 cttttgaggt gtttggagtg aataacggtt caacatcaga agacgatttt tacgataatt
    13021 taggtactca actatgggga aacatcaagg aaatgttaga agaaaatatg acagcaaatc
    13081 ttaacggaga acagcctgtt attgaattgc cttctgatag ttcgttaatc aaagaattaa
    13141 gtactcgcaa attcaaaatg acaagtagaa gtcgtatacg tttagaaagt aaagatgata
    13201 tgaaaaagcg aaatattggt agtcccgata ttgctgacgc actggcttta gcgttttatg
    13261 agccaccaag tcactatcaa tttattcaat tttaggaggt gagcttttga ttagtacagt
    13321 attatcttta gagagatata agaagctacg tgttaaatat gcaacacaaa ttgaagatgg
    13381 aatgtttgat ccgaatggtt ttatagaaga tatgaaacca ttttttgctg atcgcgaaag
    13441 aaaatatcta gcttatacta gcgaaaagaa tgaaatagat aatagaccaa agcctaacac
    13501 tgatattgta aaagttaata ataaacttca tgctggtatg tattcaattg tcgtagacca
    13561 agcagtcaat cattttactg gtatacctat caaatgggat tacgatgtat cggaacaaaa
    13621 aagaacactc attcaaagat taaaagataa attcttaaaa aatgatatag aacttccgac
    13681 agttccagaa gcttttaaca aattaacaag caatcttgat tccatgagat tcgcaatgct
    13741 tgattctgaa actgcaactt ttcaaggagc ttgtggagta gctttccgat tgctagagcc
    13801 tgtggaagaa gatgatggtt ggaagttaag agcaagtaat attgaacctt ggagagcaga
    13861 aagatatgga aatgccggaa tctatatcaa agaaaagtat gactcttacc agaaaaaatt
    13921 ttttcaagaa atgaaagtta tgacaagaaa taaaattctg acatatgctt gctatggtga
    13981 tttgaatttt gttacaagcg gaacgttcaa aaaaattgat gagaccgata accctttggg
    14041 gacgattacc ttggcagaat ttaaaaataa tacgaatcgt tattgtgatt ttgaagtagc
    14101 tgaggaaatt ggagatgcaa ttgatcgtgc tttgtctgat caacaaaacg aaatcgaaca
    14161 atttaagctt gcgtacatgc ttgttactgg cacaacaatg agcaaaggca ctgctaaaga
    14221 aatgatgaat cagctaggga ttatcaactt aaaagcccct actgcaaaag ctgaatatgt
    14281 cacaaaaaat ttagcaaaag atttcaatga atatcatatg gatctattga aaaaacagtt
    14341 ttacaccatt tgtaaagcaa tcgattttaa cgatgaggtg tttaaatcaa acagttctgg
    14401 agaagctcgc aagtggcaaa tcattagcct agaagcgaaa acaaatacta aagagcaata
    14461 ttttagagaa ggattgaaag aatgcgcaga aacaattgct gcttttctta agtttcatga
    14521 caaagtagaa attgagcctg aaaaaattat ttttactttc tctagatctt tgccaactga
    14581 tttaagttat ttagctgaag ctttacctaa acttgcacca tacgtatcca aacgaactat
    14641 tcagagccaa attccatttg taacagatgt tgattatgaa aatgaaatga tggaattgga
    14701 aagtggaagt gcttatccag atagtgaata caattttggc ggaggtggca atagtgacga
    14761 tagaaacgaa gtattggacc aaacgtcgag aactggagga tcaagcaagg ctcaaacaag
    14821 aaaatcagac acttaaaaaa ttaactagtg tatttcctga agcactgaaa gagatacaag
    14881 caaaactatt atcacaagct gacttacaca atatcactta tccagaaatg atggagtttt
    14941 acagtacaag taatcagaaa aaatatcgtg aatatgtgga aaaaaattat aagtcattaa
    15001 aaatgtatga tgcaaaatac aaagagttta tcgatgaatt ttttccacca tttgactatg
    15061 caaaagtcaa tcgcttatta caaatacgat cagatgtatt taaaattctt gcagaatatg
    15121 cgatggatgc agatgtgaat caatattttt ctgatcgctt agaggaaatt cttcaaagaa
    15181 catattcttc taatgctaat gtttttgttc aacttttaaa cgttgatata ccaaattatt
    15241 taccagaaaa tgaaattaaa cattatttga actatccatg gtcaggaaag acattttcaa
    15301 gaagactttg gggaaatatt tcatcgcttg aacagaaact ctctaatgct attgtaaaaa
    15361 gtgttgctag tggagaaggc gttatacacg cattaaacac catgagatta gactcagaaa
    15421 tttgtgacat gtttaagtta gaagaatcaa agtataacaa agcgatagaa aatctcgttc
    15481 gaacggagta tgcaaaattt gcacaagatg gtattgaaaa atcatattta gaaacaggta
    15541 ttgaggaata caacgtattg actgcaaaag atgagagagt ttgccggatt tgtggaggaa
    15601 aggcaagtaa gaatccctat aaactgaaag atgctgtcat aggtgaaaat cgagcacctt
    15661 tccatagtcg ttgtagatgt acggatgttc ctaatttacc aaaattagga aaggatattg
    15721 atgaagaata tgaccgttta tttggcgatt tattagatga gtttgcacat gattcttttg
    15781 gaattaattt gaaacggagg aagtagaatg aaagattttt ttgaagcagt actaacaatt
    15841 aatgtaaatg ctgatattgc agaagcctac aaaacagcta ttgagtctga gaaccatcct
    15901 aatggcttga gagaccattg gaatggcaat tatgcctacg tggttattgg cgatcaaact
    15961 gttaattatc aagataatac tccagttgat aagaataccg ttaatttaac gattcaatta
    16021 ttatctcatt cattaccaaa tttaaaagaa acagttgatt ggtatgaaaa gatgggatgt
    16081 attgttgtta gaactgacta caaagaagga aagtctagta attaggcttt tttattttgt
    16141 ccgaaatgac gttaaactag cgcaatactg ggcttggttg aatggtgggg cgcaactatt
    16201 aaaactcaaa gcaatgcggg gcgtgaaaac gaatcgtggg gcgaaaggag aatgagcatg
    16261 aaacacaaat cattaatgcc aatgaatttg caatactttg ccgaaggtga cgatcaaaag
    16321 ttttcttttg atgacttcaa atcttttgtg gaatccaatg aagaagctca aaaatttatt
    16381 cagtcacaat ctcaaagtgt tgccgacaaa caattggaag cttggaaaca aaataactta
    16441 gataaaatta aacaggatac catcaaggaa tatgaggaat ctaagaaaaa caagtcacct
    16501 gagcaaattc aactggaaaa actacaagct gaatttgaag cagaaaaagc gttgcgtgtg
    16561 acaagtgata ataaagcatt tgttgcagaa caaattgctg gattagaact agatggagag
    16621 ttaaaagagt ctatttctca atttatgcta aataatcttg ttagttcgga tacagatttc
    16681 actaagaatg ctgttgaagg ttttacaagt gttttgaatg caattaatga gaaacatgca
    16741 gatgcattaa aagaactaca aatgaagtct gcatttggtg gaactcaaca atcgaataac
    16801 caggttcagc agaacaatga aacattaaca aatccagaag aacaattagg acaaattctt
    16861 caacaattta actaggagag tgaaaaatta tgaaaaaaac atctttaaat aatttagagt
    16921 atttggatat ttcaccagcg attaatgcta tgcaagtacc aaatacacct tttttaagct
    16981 atttacttgg tgctggaaaa acagagccag caaactcgac agaaattaaa tggcgtgaat
    17041 atgatatcaa caacgatgat tcttctgaaa aacttgaggg cggagaatat ccagatgctg
    17101 aatcaggtcg aacttggttt aacaactata ctgaaatttt tagaaaatca acctctgtat
    17161 ctggtacatt agatgctatt aatgtgaatg gagtcggaaa tgaattaact aatcaagtag
    17221 cactacgtgg tatggaaatg aaaattgatt tgaaccgaaa attgattact ggtgtaaaag
    17281 ctgatgaaaa tggttctaaa ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag
    17341 caaataaggc agaaacagcc actgcgggtg cagtaacaag aaaagatatc gatactttat
    17401 ttaaattgat gtatgaaaaa ggttatatgg gagaaaaact atgcttgatt tctccagata
    17461 tgcaggagtt aatgactgat gagttagatg gaaaatcaac aaaaattgtt cagttcgggg
    17521 aaagagtaac ttttggattg caaattggaa atatcgtgtc taattacggt acaggtattg
    17581 ctctactaga accatcattg ccaaaaggaa caattgccgc aatcgatact aattatgtga
    17641 aactacgtcc attacgtgaa tggagagcag aagaacttgc aaaaacaacg gattctagac
    17701 gtatcggtct tgtaggtgaa tactctcttg aatacaatgc ttcaaactct ggggcaattt
    17761 taaatttaaa gtctgaataa aagggagtta gtactccctt ttttgatagg aggaattaca
    17821 gtggtaaaaa aagatgaaac taaaaaagat gaagtcgtga aatatagagt aggtaaaact
    17881 aaaaattttg ttgggtttgt tcatcctaaa actcgtagat ttatcacagc agattcaaat
    17941 aacgaattta tcatttctat agatgataaa gaagcaattg caattttgga agatgcaatt
    18001 gatgttaatg aaatttagga agtgatctga tggatgaatc gctaaaaacg gaaatcattg
    18061 agtctacaaa agaagatttt ccagatttga gtgaagaacg cataactaat ttattagaaa
    18121 taattttgct agaaattgaa tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt
    18181 taaaaagcgt gattagtgaa gtgttgtata aaataataaa aaatgaatca gaaaaaacag
    18241 tatcttcagt tagacgtggc gatacaacga ttagttatgc ttcaacaaca aatgatgtta
    18301 gtgagttact tctaggctat ggtgatttga tacgaagagt tattggttgt ggaggattgg
    18361 agttttttta atgaatgaag cagatatttt agaaactact tatgaagata gttgtattat
    18421 tgaaagactg acggacattg aagattctaa tacaaatatt actattcaag attacaaaaa
    18481 agtatatgat aatcctattt cttgcgctct ttcacaaggt caaatcgatg gactagcagt
    18541 catagaagat ggagagatgg taaatgtttc aactgacaca tataaattat ttgttcatcc
    18601 taagattaaa ctaaaaaaag gagatcgaat aacaataact caaaaggcca gtggcttaat
    18661 tttttctcta tttgctacta agccttttta ctatcctagt cattgtgaag tgaatttgat
    18721 aggaagtgag aaaaatgggt aatctcaaat atgaatcaaa tgcagaaaag atcattgaga
    18781 attttaaaaa tatgactgta attgctcaaa aagaaggaat atccttcgtt aacgattcaa
    18841 tgaataaagt cgttagttta gctaaaccat taactcctgt aaaatcaggt aatttaaggc
    18901 gaggatatcg tgtagtaaaa gctagaaaac tatcaagcgg tcgaattgtt ggagaagtta
    18961 taaacaatga acattatttt aaatatgtag aagaaggtca taggactaaa aatggtggct
    19021 ttgtcaaagg gaaattcatg ttaactcgtg caacgaatct tgcaaatatg tcttatattc
    19081 ctcgaagatt taaacaaatg gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat
    19141 gaaatcattg ctgcaatcag tagcaaatta aaagaaatct atcctgatgg gacaatatat
    19201 cttgattcag ttatgcagtc aactaaagat ttttatttcg ttttatcagt aatggaatct
    19261 ggaactgaaa atgtaggaat tgatattcaa aatgtttctt tcttaattga tattgcattg
    19321 attgataata accctaatag aaatttaatt aatgaattag tctcacgttg tgggactttt
    19381 tttaatacga ttacaatcga tgagcaaata ttatttccaa aagcctattt acctgatgaa
    19441 gcagatggtg ttcaacatat tcgttttaca ttagaatttc cacaatatat tgaatggagt
    19501 gaaagataaa tgggagaaaa aagaagtaaa accggaatta tttctgtcga aaaacctact
    19561 tggtttcctt tggaagatga gacagggaat tttccggtat atggtactgc gttgccaatg
    19621 ggaacagcag taagtatcaa accaacagct aattatgaaa caacacaaga ttatggggat
    19681 tcagtggttc aagatcaatt tacagctttt ggtggtgcag aagttgaact ggaagcaaat
    19741 ggatatactc ataaagtttt atctacaatt actggaggga aaattgttaa aggtggtgcc
    19801 ttgcgttctg gagaagatat tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg
    19861 aatgggaaat accgttatac ggtcttttat aaaggacagt ttgctctaga ttcagatgaa
    19921 acatcaacga ttgaaggaag taaagtaagt tttactcatc caacttggaa gggttcattt
    19981 gtagatgtac caggacttgg atatatgtat tcagttgatg aagatgatga aggcgtggat
    20041 aaagcaatga tcggaaattg gtttacaaaa gtagcaattc caattgaaga agcagaactt
    20101 tcaggaggta cagaataatg tcaaaatatc aaacaacgat taaattaacg aaaaaagacg
    20161 aagaaggtaa atatgagcaa gtacaattta aatctgctga atttttacca ggaacagttg
    20221 tagaagatgc tgcaggagtt atggaagaaa tgcaaacagc gactgataag caatcagtta
    20281 aaaaagcttt aagtcgtgct tattcattta ttgcagatac tttatttgag ggacaattta
    20341 ctggtgaaga ttattgcaaa ggaattgatg ctcgtgagat tgcctcattg acaggaaaat
    20401 tattgaagtc tgttactgca ggttttgatg aaacttatac agagacgaaa aaaaagtaag
    20461 tgaggcgctc aagtcacctt catttaagta ttcgattact taccgagaat tagatataaa
    20521 gacgcaatta ctcgaagcag gttggacgtt accagaaatt gaacgtactg acttggatga
    20581 gttaatgcgt ctttttgctt ttagagatgc ggtaaaagag catgaagatg ttgagtacta
    20641 tgataatttc actcaatttt aggaggtgat actttgaata acgatgacct aattctgaag
    20701 atgatactag atgaatctgg ttttactgcc ggtatgaata atgctgtcaa aaagttgaac
    20761 tcttttgatg aaacaatcga aagaaaaagt agaaatagtg gcagttcatt aggtagtatt
    20821 tggaaaatat ttgctggaag ttttctagct agcggagtaa ctagaatcgt aggagctggc
    20881 tttgatctaa tcaagggttc cataagtgga gcggttgatc gagtagatac gatgaataac
    20941 gctctacgaa atttccaaaa tatgggattt agtaattcag aaattatgaa gaatatcgga
    21001 aagaatgggc ttttatctca aggtattcaa ggacttccta ctgctttgaa tgatgcgata
    21061 agtcatgttc aacttcttgc ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt
    21121 aaagctttaa atgatggaat tcttggtttc ggtggttcaa ctgatcaagt taatgaagct
    21181 gttattcaat tatctcaaag tttctcaaac ggaaaagtag acgcacaaac ttggaactca
    21241 atgatcaacg ctcaacttgg acctactctt tctgctattg ctaaaaagat gggaattaca
    21301 atgggagatc tgaaagaagg tttatctcaa ggtaagattt ctgttgaaga attccaaaat
    21361 caattaatag aaatggatac caaaggtggc ggaggactta aatcattaag tcaaatcgct
    21421 aaagattcta ctaagggaat taaaacctct atacaaaatg caaaaacagc tgttacacgt
    21481 ggtgttggtg aggtaataga aggattaaat aaagcattag tagattcaga tttaggtgga
    21541 tttaagggaa ttattgataa agtgggcagt tcaatggaat cgttcttaaa agtaattgct
    21601 gcaaatattc ctacagcagt atcattttta agtaatttat ttaacgaaat tcaaaaattt
    21661 ggttctgctt tgaaattcat gatgcccttt cttgttcctg cagctaccgc ttttggagcg
    21721 ttcatgtttc aacttaaagg tataccagca ataataaaaa gcttcaataa ttttaagaat
    21781 gccataattg gcgttggaaa ttcattaaag attatggggg caatagctgc tgctaatcca
    21841 tttgttttaa ttattggtgc agtcgttggt gcaatagctg ttttcggtta ctttatggca
    21901 accaatgaag agcttagaaa caaggttata tctatttgga atgatgtgaa agattccgta
    21961 attggtgtat taaaaaatat aaaggactgg ggaattgata cttggaattc tgctaaagaa
    22021 atggcatcaa atgcagttga gggtgtcaaa gatgcttggt caggaacaaa ggaatggttt
    22081 tctaacacct ggaaaggtat taaggatggg gccacaggtt tatttgataa aacagtagag
    22141 acttcaagaa atgcagtcga tagtgtaaaa aatgcatggt ctaatatgaa gaaatggttt
    22201 tctgatactt ggcaaagtat aaaagattca gccagagaaa aatggaatgg aatcaaaggt
    22261 tctattatgt cagttgctgg tccattgatt acaggtataa agaatgcatt tttacatgtt
    22321 actttttatc tagagacctt atggaataat ttagttgaga ttggtaaaaa cgtctttgag
    22381 attttaaaaa atgttatact tgcgccggtt ttgtttatta cctccatgat ttctggtgga
    22441 tgggaagaaa caaaaaataa tatgattgct gtttggaata atattaaaga aagtgccata
    22501 aatatttggg aatctataaa gaatatattt gtaagttatt ttacaaatat ttattttgct
    22561 gcactcaata tttggacagg gttcaagctt acattgataa atatttggaa tgaagtggta
    22621 aatcaagcta aatcgatttg gttaaacata aagtattttt ttattaatct ttggattgat
    22681 ataaaatatt ttgcaattca gaaatggatt gaattgaagt tttctattat tcaaacttgg
    22741 atcgatttaa aatataatgc cattactact tggaataata ttaaacagtt tttcaaagat
    22801 acttggaaaa atattaaaga tacggcatac aatacgtgga tttctataaa aaataccatg
    22861 attaatacat ggaataacat taaggactct ttctggaata ttgttactgg aattgttaat
    22921 tctgctgaaa atgcatggac aaatctaaaa aatggtgttt caaatgctat taatcgggta
    22981 aaagaaatct ttaattcgtt aagggaagtc aatttatttg aaattggcaa gaacatcatt
    23041 gatggactta taaatggtgt aaaagaaaag tggaatgctt tgaaagatac tatcaaaggt
    23101 attgcaggta atatcaaaaa ttggattaaa ggagctttag atattcattc tccatcaaga
    23161 tggatgcgtg atatggttgg taaaaatatt gttcaaggta ttatcgttgg tattgataaa
    23221 gagcaaagta agttagatca aaccatgaca aatttagtaa aaacaccgtc tgttcaacct
    23281 gtagtaaatg gaataagtca cctaccaatt acacaaacaa agcaaaatat atctgctagt
    23341 gaaacaaaag aaattcattt acatttaaat gtttatggag atttacctga ttcaatggtt
    23401 cggaagatta ccacaaaaat taaaacagaa ttgacaagac aaatgaaacg agatgctggt
    23461 gcagtaggag gaacattata tgcaacttaa gagaggacaa ttttttatta atcaacatta
    23521 ttcttctgaa tttaatgtgt atattcaaaa caggcctgct tctgtttcag ctagtcgagt
    23581 aattgaattg agagagcgag aaggaaatga ttctatcatt attgataaag gctattacaa
    23641 aaatgtgact agaaagattg agtgttacta taaagcacct tcaattgata tggtgcagga
    23701 gtgggaagat cgaatcactg aatggttaga tatgtcgtct tatagtgatt ttattcttta
    23761 ctatgatgaa caatatattt atcaagctgt agtaatagaa ggacctgaat ttaaaggaac
    23821 aagaaaaaca ggaaatattg tcccttttga atttacagtg agtattcgac catttaagga
    23881 aaattacaat ggtagattta ctattcgaca aacagaaact tttgaaattt ataacccaga
    23941 gaagtattct tcaaaaccgc ttattaaatt gagtggttct ggagatgctt ctttttatat
    24001 taataaggat aaatatgatt tgaaatcgtt ggacagagaa ttatttatag attctaaatt
    24061 agaagaggct tatcgaaaat tagatggtaa tttagaacat caggatcagg tcaccttatt
    24121 tttagatttt ccatttttat atcctggaaa aaatgaaatt aaatggacga aaaatattca
    24181 ttcatttgaa ataatgccta ggtggtggag aaaagtatga aaccaagaat atatagtcct
    24241 actgaaacag atttctcaac gaatggctta ggaattttaa aggattgtac aagatgtgga
    24301 atatatgaag tagctaatgg aaaatatgaa ttagagttgg attatccttt aggaactaga
    24361 tttgatgaat attttgaaaa tgactatcaa ataaaagcaa agccaaacga tcaagaagag
    24421 tatcatatct tttttattga tgataaagat atagatactt ttttaaatac agtaactatt
    24481 tatgctcaga gtcgtacaaa tcgacttgga agacgggtag tcactcttgt ggaagtggac
    24541 tctaaaactg gtcaagaagc gatatcaatt attgaaacta aaatggataa aaaatctgac
    24601 atacgacttt attctgatat tacggctgtt tctagcacaa tctttgaagc gagaaatgtt
    24661 ttaaattgta ttgctggtga acaaggatca ttgcttcaat attggggcgg agaaattaaa
    24721 cgtgaaccat ttaagctttc tttgttaaag cgaagaggtc gcaataatgt tggaacgatt
    24781 cggtatggaa aagatatgtc tggtttgaag gtcaaattag attggacagg tataaaaaca
    24841 agaattattc catatgctga tcctcaaagt gaagtaggta cgactaatcg aatttatggt
    24901 tcgccagtag atagtgccta tattaataac tatcctgatg tctatacaga acatgttcag
    24961 tttacagaag aacaaggagt aaaagacatt aagagcttaa ataaaatagc taaaaattac
    25021 tttaaaacga tcaatcctgg ctgtgataag cctaaagttt ctattacggt tgaatttgat
    25081 aagttgactg atagtgaaga agcgaaagaa tttgcgaaga ttagaaatta tggtctgttt
    25141 gatacattta aaatatatca taaaaaatat aatatttatc ttgaatcaaa ggttagtgga
    25201 ctacaatacg attctttatc tgaaaaaact ttgaaattag aagcaggaga cattcaagtt
    25261 gctttttatc agcaacaagc tgtaactatt caggataaat taaaagatta tgcaacgaat
    25321 aattatatga gtgattttaa tgattatgtt tcttcaatga ttacaggtca aggcagtgca
    25381 ggaggatatg tagttttatg gccaaaagaa aaaccttcca acatctttat tatggataat
    25441 ccagatttag aaaaggcaag agaagtacta agaatgaata aaaatgggat tgctttttct
    25501 aagaaaggat ggcgggggcc ttttaattca gcttggacat tagatagtat atttaatgcc
    25561 aattttatac aaactggatt aatcaaagca gatatctttc aaaattcttt taacaagact
    25621 ggagatgtat tgaaattagt taatggacta ctccaaattt ggaacaacaa aaagaaaata
    25681 atggagttaa ctaaaaaagg aatggaattt tggaattcta agggttcaat tggaacgatt
    25741 ggaacaactg attctgttgg taatcctttt cctggggctt ctactccaac ccctattgaa
    25801 gataattctt tagttattcg tacaaatgga gacggcaaat atattttgat ttctcctaaa
    25861 gtaggaaaag gattagtttt attaggaaat ggtaaagcaa tttattttgg agacttagat
    25921 gtacaaggaa aacttacagt taaaggaaaa gaaataattc ctggacaagg tggtggtcca
    25981 tcgggaggtg gagaatctac tggtgggtat cctagtgaat taaaaacaga tgcagaaaag
    26041 agagcttgga gaat
//
LOCUS       DAFCHQ010000033        25778 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000033 DAFCHQ010000000
VERSION     DAFCHQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.033"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1835)
                     /locus_tag="LNC71_001437"
     CDS             complement(<1..1835)
                     /locus_tag="LNC71_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6466513.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="LNC71_001438"
     CDS             complement(1832..2536)
                     /locus_tag="LNC71_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6466514.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="LNC71_001439"
     CDS             complement(2548..4857)
                     /locus_tag="LNC71_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBK6466515.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="LNC71_001440"
     CDS             complement(5052..5516)
                     /locus_tag="LNC71_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466516.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="LNC71_001441"
     CDS             complement(5516..6142)
                     /locus_tag="LNC71_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBK6466517.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="LNC71_001442"
     CDS             complement(6149..6514)
                     /locus_tag="LNC71_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBK6466518.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="LNC71_001443"
     CDS             complement(6504..6842)
                     /locus_tag="LNC71_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466519.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="LNC71_001444"
     CDS             complement(6832..7158)
                     /locus_tag="LNC71_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBK6466520.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="LNC71_001445"
     CDS             complement(7136..7417)
                     /locus_tag="LNC71_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBK6466521.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="LNC71_001446"
     CDS             complement(7419..8783)
                     /locus_tag="LNC71_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBK6466522.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="LNC71_001447"
     CDS             complement(8796..9365)
                     /locus_tag="LNC71_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBK6466523.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="LNC71_001448"
     CDS             complement(9337..10524)
                     /locus_tag="LNC71_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBK6466524.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="LNC71_001449"
     CDS             complement(10588..12315)
                     /locus_tag="LNC71_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBK6466525.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="LNC71_001450"
     CDS             complement(12312..12764)
                     /locus_tag="LNC71_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBK6466526.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="LNC71_001451"
     CDS             complement(12876..13256)
                     /locus_tag="LNC71_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBK6466527.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="LNC71_001452"
     CDS             complement(13253..13639)
                     /locus_tag="LNC71_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466528.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="LNC71_001453"
     CDS             complement(13641..13919)
                     /locus_tag="LNC71_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466529.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="LNC71_001454"
     CDS             13967..14695
                     /locus_tag="LNC71_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBK6466530.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="LNC71_001455"
     CDS             complement(14841..15317)
                     /locus_tag="LNC71_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBK6466531.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15624)
                     /locus_tag="LNC71_001456"
     CDS             complement(15277..15624)
                     /locus_tag="LNC71_001456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466532.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="LNC71_001457"
     CDS             complement(15611..15796)
                     /locus_tag="LNC71_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466533.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="LNC71_001458"
     CDS             complement(15797..16069)
                     /locus_tag="LNC71_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBK6466534.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16269)
                     /locus_tag="LNC71_001459"
     CDS             complement(16066..16269)
                     /locus_tag="LNC71_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466535.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16394..16606
                     /locus_tag="LNC71_001460"
     CDS             16394..16606
                     /locus_tag="LNC71_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466536.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16639..16833)
                     /locus_tag="LNC71_001461"
     CDS             complement(16639..16833)
                     /locus_tag="LNC71_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466537.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16846..17028)
                     /locus_tag="LNC71_001462"
     CDS             complement(16846..17028)
                     /locus_tag="LNC71_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466538.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17025..17165)
                     /locus_tag="LNC71_001463"
     CDS             complement(17025..17165)
                     /locus_tag="LNC71_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466539.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17655..18020)
                     /locus_tag="LNC71_001464"
     CDS             complement(17655..18020)
                     /locus_tag="LNC71_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466540.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18060..18362)
                     /locus_tag="LNC71_001465"
     CDS             complement(18060..18362)
                     /locus_tag="LNC71_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBK6466541.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18362..18661)
                     /locus_tag="LNC71_001466"
     CDS             complement(18362..18661)
                     /locus_tag="LNC71_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466542.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18654..18818)
                     /locus_tag="LNC71_001467"
     CDS             complement(18654..18818)
                     /locus_tag="LNC71_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466543.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18824..19093)
                     /locus_tag="LNC71_001468"
     CDS             complement(18824..19093)
                     /locus_tag="LNC71_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466544.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19105..19854)
                     /locus_tag="LNC71_001469"
     CDS             complement(19105..19854)
                     /locus_tag="LNC71_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6466545.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19851..20543)
                     /locus_tag="LNC71_001470"
     CDS             complement(19851..20543)
                     /locus_tag="LNC71_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466546.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20549..21220)
                     /locus_tag="LNC71_001471"
     CDS             complement(20549..21220)
                     /locus_tag="LNC71_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBK6466547.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21213..21554)
                     /locus_tag="LNC71_001472"
     CDS             complement(21213..21554)
                     /locus_tag="LNC71_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466548.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21732..22430)
                     /locus_tag="LNC71_001473"
     CDS             complement(21732..22430)
                     /locus_tag="LNC71_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBK6466549.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22517..22987
                     /locus_tag="LNC71_001474"
     CDS             22517..22987
                     /locus_tag="LNC71_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466550.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23030..23209)
                     /locus_tag="LNC71_001475"
     CDS             complement(23030..23209)
                     /locus_tag="LNC71_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466551.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23196..23534)
                     /locus_tag="LNC71_001476"
     CDS             complement(23196..23534)
                     /locus_tag="LNC71_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBK6466552.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23550..23753)
                     /locus_tag="LNC71_001477"
     CDS             complement(23550..23753)
                     /locus_tag="LNC71_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6466553.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23768..24544)
                     /locus_tag="LNC71_001478"
     CDS             complement(23768..24544)
                     /locus_tag="LNC71_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBK6466554.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24843..25163
                     /locus_tag="LNC71_001479"
     CDS             24843..25163
                     /locus_tag="LNC71_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6466555.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25181..25612
                     /locus_tag="LNC71_001480"
     CDS             25181..25612
                     /locus_tag="LNC71_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBK6466556.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         7252 a         5323 c         3996 g         9207 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaattt agtaaaactc gttgaacaac gttttaaaga
    16441 atcaggatta gaacaattaa aaaaagataa ttctccagaa ttattttcta attttaccga
    16501 agacgacatt cctactctta aacaattaaa atattattct gattattgga cgcaaactcg
    16561 tgagaatgaa ttaaatttag aaaaactcat tattaaaaag aaatagcaac tccagcccac
    16621 acggctggtt ttttagtttc aacttccctc cttacgggtc aaaatcatcg gttccggcat
    16681 aatctgatca tcctttagca acaaatcagg atattttttc cttagcttct tattgttcgt
    16741 gtaagttttc aatagccatt ggttggcttc gcttttgctt tctccagaaa aataaatttt
    16801 ccctgtttgg atgcctgcaa gttgaatcat cattttcacc tcaaatcaaa ttttttctgc
    16861 tagatggagc aaaagcaatc cgacatcaag aatcttttga cccattattt cagctacttt
    16921 gctcggctta actccttgag aaaagagcaa gcgtgcttgc tctatctctt cgtgagtcca
    16981 aataaatttg gcttcctcaa ggataattac tggatcagtt gctctcattg ttcctctgga
    17041 aaaatgatgt cattcttatg cttggaccag gtatctgcat atggaacaaa gtattgtcta
    17101 gctaattcga tttgatcaat taaagcagtt tctgataatt catggtctgc agcaatttca
    17161 ttcatactat cgccttcatc taaacagata agaccgttga cgttatcgat ttttacattt
    17221 gtgactgctt gactttctcc acgctcataa tattctacag ttgatactga tacatcagct
    17281 tcggagttta attgcagaat ctgttactgc cgggataatt taatattctt ttcataattc
    17341 accttttttc ctactgacat cgaatatttt tcatgttatg attagccgtg aaaggagagc
    17401 tatattatgt gtttacacat gatgtcctta gctcattcgg ttctttggaa gcttttttgg
    17461 aaataactct caggttcttg acgcaaatct catttattct ttgaaacaaa aaacttccat
    17521 tttccgagag ctttggatca cttcaaaaga gatttgcatt tatatttctg tgaccttgag
    17581 tcgcgtaaag aaccttacag caatggcggc aattccactt tcaggaaacc gccttttttc
    17641 attgatattg gtggttagcg gactacccat tttctttcgg cagtgccaaa ccacaaatga
    17701 tctgtgttga tgtttaggta gttgtatacc agataagcta aatccaaagt gcctgcatct
    17761 tcaagacgac aatattccaa aaattctccc atagctggac ataaaaatcc tggagctatc
    17821 atatcccaac agttaatagt gcaaagaaat tcgtttagct tctgaacgtt ttctttggtt
    17881 tcacgaatta caacatactc caattcttct ccgctggcag agcattccaa aacttcaaat
    17941 tgattaagaa atggtagaac atatgcttca aacatcaaca ttccatcttt ctgtttttgc
    18001 acttgttttg catcaatcat gtctatcctc ctattcagtt atttcttcca ttaatggaat
    18061 tatttcaggt cactggactt aacgaaaaca ccattgacca tctttccagt ccgacctttg
    18121 atttcgtcat aagcaaaatc caagcactcg tataaatcca tatcattttg cattgccaag
    18181 atgatcaagg ttactactac atcgccaatt ccgtctctta aaccatgttc atcttttcta
    18241 gctagagaag cggcaacttc cccgatttct tcgaccgttt ttaacatttg cttgctgaaa
    18301 tccgcttgat ccaatccctt atcttttgcc cactgctcta ctttcgcgat tagttcgtcc
    18361 attatttctc ctccacatac ctaaactgtc ttccttttga atcaatccat aagctcctag
    18421 ctctatccca aataatattt ttgctcagac cagtaatttc agataactgt ttagcggtac
    18481 ccgttactag aattcgatca ccatgccaga ttgcaatctt tctcggcgtt ctccgtttgg
    18541 gcttttcagt ccacattgat ttaccgagct tttggacttc tgcaactatt tttttatctt
    18601 cctgccaatc ttctgaataa gtcaattcga taattcgctg cattgccgct ttcttatcca
    18661 tcccgacatt ctcctttcag taatttgagt acttgatcaa gtgcgctctc acgtccgcca
    18721 tgaaatgtgt tgagccactt gtcttcgtac gacacacttt gtcttaaagc ttcttgatgc
    18781 attaattcga tctgtgctgt aaatgtcttt aggtccatct tgtttacacc tgctcaagtt
    18841 cattaagatg tttttgcaag cctttaacac aatcaatgaa tagtaatttg gtatatgcta
    18901 aatttcttaa ttgcgttcca tcgatataga gtgcgaaata gtatctgagt ttactccaac
    18961 tcgaacgatc attcttaatt ccttcaattc cagcttcttc gagttgatca tatacgtctc
    19021 tcaggacgtc taattcctcg ccctttttat aattcgctat ttcattaatt agttctagat
    19081 aatcgatctt catttttcca cctcttagaa tggtgctttt gattgtctat tagctcgttc
    19141 tagcgctttt ttcttgagat aggcttcttg gtcgattgcc cattcaggaa gcttctctcg
    19201 tcttcctgtg cgcttgtatc cactgcttgc gttcttaggt tcactttttt ctttccttgc
    19261 ccaacttcga atagttgcca aatagttttt ataagtctta ccagatgatt cacaatactc
    19321 tgacagtcgt tcgattcgct cttggtaatc attagggaat tctgttttga gtttctccat
    19381 ctgctcatct gacaaaagaa catttttata ctctccgtat ttatgacgga cgggcttagc
    19441 cttcgatttt ttcgaaggcg gtaactctct tatatattct tttgtattat taaatgtatt
    19501 attaatagat gtattattat ctttgacttt ttcgtcaata ggggtattgc gtttttcgtc
    19561 aatagggtat tgattaattc gtaggtacct attgattatt tgattggtac cctctttgta
    19621 aatgatttcc cgattcaagt atccaaactt aatcaaatca cttacccatc gcgatatggt
    19681 ctctttattc acaccatata aatctgcaaa gtactcattg cctgcccaac aaaagcctct
    19741 ttcattacac aaggccgtta tctctccgta taacaactta gtatttggtt taagtctttt
    19801 gtcgtacctt acgttggctg gtataatcgc ataataactt cgatgttctg tcatttttcc
    19861 cctccaatat ttaacttttt gattgttttc tggtttaatt tgatcccttt gatttgatat
    19921 ttatttttga aattaatcac acctattttg tgcttctctg tgtgatggat tctgcagagt
    19981 gctgcaaatg tgtactctgc atgatcaact tctttgcgct ttcgtcttcc tagcgctttg
    20041 tcaaagtgat cgatgtcagc tcctgttttg ccacagatgc agcagactct ttttgtgatg
    20101 catttgtaga agtaatattc ttgattcgca ggtaaaatct catagccttc tttgaaagga
    20161 atatgatgtt caaagatgaa atctaagatg atatttgcta agacattggc atcactcaca
    20221 gttgtattcg attcgtcttt caggcttatt ttgcgccctg tgacaccttc aaaacgaaag
    20281 tagaagaatt ccttccagaa gtccgttggc atgcctgtat cgataaaaat atcgcctatc
    20341 agcgcataga tgaagtttcg ttgctgtacg gtgaaacgtc taggatcaat aaaacgaatt
    20401 tcaatgactc gatcaccatc gtagccgtca tacatcgtct ttagtcgatc aatgttcact
    20461 tcctcattga tggttgcgct tatgtctttt cctttgaact ttttcagaac cgctgaatat
    20521 gaatcgatta atggtttaaa cactcatatc acttcttatc taattctttt ctcttagctg
    20581 ctattgctcg ctccatcaag gcacattgct catagcttaa ctgttcaata gtttcaacgt
    20641 tatcagctaa gagccctaat ttatctgtct gctcattaac atattcgatt aaggttttgg
    20701 tcatatcttt acccatctgc tcattgaaag cttctagaat cgtctctagc atgtttaatt
    20761 tctttgtatc gattctaggt ggtgttggaa tatcttcccc ttgaaataca tataatccca
    20821 gtccgtgtag agccaatgct ttcacaaagc atcgcttcaa tgagttattg atttgcattg
    20881 catttggttt aacaactggt tggtttcgat aatctaaaac aggaaataat tcggtttccg
    20941 tgtgtccttt aaccgttact gagacagata cataagtccc agtttcatcc ataagaaaag
    21001 gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg tagtgtttgt
    21061 actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc cacgcgtgag
    21121 cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac ttacgactaa
    21181 aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc ctccatttca
    21241 gcaatgtatt tcttacctgg tccgtaataa gagatatcaa tcaagttatc tctgtcgtac
    21301 tcttctagcg catcaatcaa gccatcttcg atgacgtaaa tatattcagg tttattcgaa
    21361 tgcttcgata gatggataag ataaacatga tcccaaatag ttacatggtt gcctaaatca
    21421 tcttgatccc aagctagttc ttcattcgtc aaaagatttc gtctgatttt tcgaccactt
    21481 gtttcctcaa ttttcggctt gccccaatca ggatcaatca aatattgatc tagagtggaa
    21541 agttcttttt ccatatgcta aaatctcctt atgatgtgtt ttctttgtga ctctttgctt
    21601 gccggcggag tcactttttt atttgttgcc atgctttttg cttgtcaata tgttgttggc
    21661 ttaggatgat tggtttatag tatttccacc agcaattagc aattgccgtc cctattctta
    21721 gcgcttcagc tctattcatt gtcatcacca aaaagtctct gttgtctgtt cagttgatca
    21781 atttccatac ggatcgcagt ttctggcaac cacatttcaa taaatgaaac agcatcatcg
    21841 aatctcttac gaggtaactc gccatatctt gggattgaaa aggtacgttt aaattcagac
    21901 caaaattttg agaatacttt tttgctgatt tcttcataag ctcggctttc ttttccccct
    21961 agaactccca taactttcat atttcctttt tgcttaattt caaactcttg ttgtccgcta
    22021 attcgcatag tatctttaag catggaaaca tcttttttaa catctttcat ttcttctaat
    22081 tgatagatca tcatatcttc aattgtttga ggaacagtat tcttgcgaat aacatcttcc
    22141 atttcgttga atgcttcaat gtatttttgt ttgaagtaga tagctttctt tcctgtaaac
    22201 cccatagcca gcaagaaaaa tccatctcta ctaatgaaga aaactcgtcg atttctgcca
    22261 tatgaatctg gttcattacc ttctacaaac atctgtccaa aattggacac atcttttttt
    22321 agtgcatcaa tatctcttaa aacatgttga tgttttttct cgaagctttc tgccacttgt
    22381 aagctcgtag tcacagcttc tttatttttc aaaattacta attcttgcat tatttcttct
    22441 ctcctttttg gtataattta gttaaaaact ggtggtgttt attttgaatt tttttcactt
    22501 aacaattttt caatggatgt caataataag ttttatattg agtacgtttt taacagggat
    22561 aaaaataaaa ggaatgcgcc ctcaactaga agttgaactt aatgcttctt acttcgctgc
    22621 agatatgatt tatacaaaag taatcatttc taactattca acagaaccag ctatgttagt
    22681 taatttagaa ctttcatccg ctaacttaaa ccgcagatgg tcagctactc catttaagaa
    22741 attaattgct aaaggaggat cagtcgatga taatagaata tactctgaag ctgtaccgct
    22801 aaatattcct cctaaaagtg ctatatcttg ttaccttgca tttgaagtag ggaaaattaa
    22861 ttttaataaa ctccttaatc accaaacaaa aatgatattt actctaaata gaacccaaat
    22921 ccacaaaatt gttgatatca aaaatacaaa cttcccaata gagaaattag tgaaagaatt
    22981 aaactgagta atagtaatat acattctagt ttctcttttc tattcatttt tagtcagccc
    23041 cctcggttgg ctttttcgct ctgtactcag cttcagcttc atcaagcccc ataaaaatcc
    23101 aaaccatgta aacaatcgtt cctatcaacg cttgcctgct tccccaaagt cctaaagcgt
    23161 agatgattag cggtgcgctg aacactagcg ctctattaaa ttttcccatc cgcttgcctc
    23221 cttaagattt ccgaaaaatt tgtttctaaa aattcaagtg ttttacttct taaaaataga
    23281 tatgtgtctc ttccttcaac tggataatag acaaatccat ttttgttttt ctcgatatcg
    23341 ataatatttc tatatcttgg gttctttaaa actctagaag taaaccaatc atattttctg
    23401 ttaatccgtt ctagcacttc tggcaacgtc atccatctac cagtatcatc agcttttttt
    23461 aactcctcat aatccacttg ggagataatt acatagcctt ctggaattgg gatttttgct
    23521 tctagatatt gcattagctg ttcctccctt tacaattcgt acatagtgat aatcgaatct
    23581 ataattctgt ttgcttctgc agaagtcttt ttaccgttta aaattaaaga taagtagctt
    23641 ttgctaattc caaatctttc agcaagcatg gtgtaagtta agaactttga actttcgaca
    23701 tattctttga ttttttctct atctcgttga gtgatttctg caatgtcagt catactaaaa
    23761 ctcctttcta accaatttcc tctaaatcca tttgagggta atatccttct ttttttagta
    23821 attcgtagat aaatagacgc cctttctgtg tccatttggt attcattaca attttagtgc
    23881 caccatcggc tttcgggatc tcagttgtat gagattttgt gtatccttgg ttcatgtgtt
    23941 ttttgcacaa taaccattgg ttaccgactt ttttctgaat acctagttta tgaagtaatt
    24001 tattcatctg ttgtggagac atcccataat ctgctgcaat ctgactaatt gttactgaat
    24061 ctgtagaaga taatatgcta tctaaatagg agattttagg ttcgtactct gcaatctttt
    24121 gttctgcgat taatcttcca gttctttctt cttttagttg agttgctaat tgaatgattg
    24181 tatctggatt aagcaaagct tcttctactt tttctggagt tagataacct ccatgttttc
    24241 taattgctgg caacacttca cttgttaccc aacgtttaaa ttttttggca gagggaagtt
    24301 ttgattttaa gattaaactg tacaaaccag attcgttgat gattgtcatc tctcttgatt
    24361 gacctgaggt cgtgattcgc gaccccatct tatcttccaa atctacatgc cgcgacaaag
    24421 catctttagt gtttgaataa cccaaaacac tcgcaacgtc tttgcctaca aaatatggtt
    24481 catcatttac taaaattgtc cgaacttcgt tttgttcgaa attaaaaatt tgcggtgtgt
    24541 tcatatttct cattcctttc ttttatattc gtaaacaaat ttaacaactt ttttctaaat
    24601 tcaattgaca gtatctgagt aatactctat actaagagca taattaaata agacataaaa
    24661 acattgattt taaagctttc ttggcggtct gcatttacat atcaatagtg ttttttgttg
    24721 tctttttagt tgttaaatct gtttacaaga tatagtatag ctcattactc agaattagtc
    24781 aaattatttc tgcgtatttt tctaaacttt ttttgtaaac atttagaaag gttgttttat
    24841 cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata tctatacggc
    24901 agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa aataatactc
    24961 ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta gactaccttc
    25021 taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac gatactgttt
    25081 tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta
    25141 aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga aattgaaaga
    25201 aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc ctggtttcta
    25261 ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc
    25321 ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat tttcagtttt
    25381 gtacgatact tctacaactt ttcactcaaa atttgaaaca gaagcagata aaatagctat
    25441 tctaatttta ttaaatatct atattgagaa cgaactgact gatgaatctc agtttaatct
    25501 cgaaaaattt atggagtatt attctattca gaataagctc agatacactt gttatgctgt
    25561 ctgccaatgc tattttaaga aaaaatattc ttacgcaagg caatatgtat gaatacatca
    25621 agaattatta aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct gcaacacggg
    25681 ctctttcctc atatatgagc ttctacaaaa aaaatcatat cacagaaatg aggaagaaaa
    25741 aatggaaaaa gagattttaa taaaatctcc aggaaaaa
//
LOCUS       DAFCHQ010000034        25706 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000034 DAFCHQ010000000
VERSION     DAFCHQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.034"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..509)
                     /locus_tag="LNC71_001481"
     CDS             complement(132..509)
                     /locus_tag="LNC71_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466557.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="LNC71_001482"
     CDS             765..1493
                     /locus_tag="LNC71_001482"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBK6466558.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="LNC71_001483"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="LNC71_001483"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBK6466559.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="LNC71_001484"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="LNC71_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBK6466560.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="LNC71_001485"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="LNC71_001485"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBK6466561.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="LNC71_001486"
     CDS             4628..6067
                     /locus_tag="LNC71_001486"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBK6466562.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /locus_tag="LNC71_001487"
     CDS             6090..7142
                     /locus_tag="LNC71_001487"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBK6466563.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="LNC71_001488"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="LNC71_001488"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBK6466564.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="LNC71_001489"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="LNC71_001489"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBK6466565.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="LNC71_001490"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="LNC71_001490"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBK6466566.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="LNC71_001491"
     CDS             10661..11575
                     /locus_tag="LNC71_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6466567.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="LNC71_001492"
     CDS             complement(11708..13273)
                     /locus_tag="LNC71_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6466568.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="LNC71_001493"
     CDS             complement(13266..14717)
                     /locus_tag="LNC71_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6466569.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="LNC71_001494"
     CDS             complement(14731..15747)
                     /locus_tag="LNC71_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6466570.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /locus_tag="LNC71_001495"
     CDS             15967..17055
                     /locus_tag="LNC71_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBK6466571.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /locus_tag="LNC71_001496"
     CDS             17109..18635
                     /locus_tag="LNC71_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBK6466572.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /locus_tag="LNC71_001497"
     CDS             18637..19842
                     /locus_tag="LNC71_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6466573.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="LNC71_001498"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="LNC71_001498"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBK6466574.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="LNC71_001499"
     CDS             21340..22764
                     /locus_tag="LNC71_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBK6466575.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="LNC71_001500"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="LNC71_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBK6466576.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="LNC71_001501"
     CDS             24337..25314
                     /locus_tag="LNC71_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBK6466577.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..25587
                     /locus_tag="LNC71_001502"
                     /pseudo
     CDS             25339..25587
                     /locus_tag="LNC71_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
BASE COUNT         8322 a         4383 c         5783 g         7218 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagtt
//
LOCUS       DAFCHQ010000035        25647 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000035 DAFCHQ010000000
VERSION     DAFCHQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.035"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            250..798
                     /gene="aac(6')"
                     /locus_tag="LNC71_001503"
     CDS             250..798
                     /gene="aac(6')"
                     /locus_tag="LNC71_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK6466578.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1344..3005
                     /locus_tag="LNC71_001504"
     CDS             1344..3005
                     /locus_tag="LNC71_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6466579.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(3075..3422)
                     /locus_tag="LNC71_001505"
     CDS             complement(3075..3422)
                     /locus_tag="LNC71_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBK6466580.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            3506..4447
                     /locus_tag="LNC71_001506"
     CDS             3506..4447
                     /locus_tag="LNC71_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466581.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4448..5491
                     /locus_tag="LNC71_001507"
     CDS             4448..5491
                     /locus_tag="LNC71_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466582.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            5497..6567
                     /locus_tag="LNC71_001508"
     CDS             5497..6567
                     /locus_tag="LNC71_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466583.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            6557..7510
                     /locus_tag="LNC71_001509"
     CDS             6557..7510
                     /locus_tag="LNC71_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6466584.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            7522..8532
                     /locus_tag="LNC71_001510"
     CDS             7522..8532
                     /locus_tag="LNC71_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6466585.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(8817..9638)
                     /locus_tag="LNC71_001511"
     CDS             complement(8817..9638)
                     /locus_tag="LNC71_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBK6466586.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(9735..10013)
                     /locus_tag="LNC71_001512"
     CDS             complement(9735..10013)
                     /locus_tag="LNC71_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466587.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            10281..12290
                     /gene="metG"
                     /locus_tag="LNC71_001513"
     CDS             10281..12290
                     /gene="metG"
                     /locus_tag="LNC71_001513"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBK6466588.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(12769..13356)
                     /locus_tag="LNC71_001514"
     CDS             complement(12769..13356)
                     /locus_tag="LNC71_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466589.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            13531..14853
                     /locus_tag="LNC71_001515"
     CDS             13531..14853
                     /locus_tag="LNC71_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6466590.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            15008..15784
                     /locus_tag="LNC71_001516"
     CDS             15008..15784
                     /locus_tag="LNC71_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK6466591.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            15777..16346
                     /gene="rnmV"
                     /locus_tag="LNC71_001517"
     CDS             15777..16346
                     /gene="rnmV"
                     /locus_tag="LNC71_001517"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBK6466592.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            16339..17223
                     /gene="rsmA"
                     /locus_tag="LNC71_001518"
     CDS             16339..17223
                     /gene="rsmA"
                     /locus_tag="LNC71_001518"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBK6466593.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            17674..18303
                     /locus_tag="LNC71_001519"
     CDS             17674..18303
                     /locus_tag="LNC71_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466594.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(18427..19536)
                     /gene="ugpC"
                     /locus_tag="LNC71_001520"
     CDS             complement(18427..19536)
                     /gene="ugpC"
                     /locus_tag="LNC71_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBK6466595.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            19745..20167
                     /gene="mgsA"
                     /locus_tag="LNC71_001521"
     CDS             19745..20167
                     /gene="mgsA"
                     /locus_tag="LNC71_001521"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBK6466596.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            20490..21026
                     /locus_tag="LNC71_001522"
     CDS             20490..21026
                     /locus_tag="LNC71_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBK6466597.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(21066..22829)
                     /locus_tag="LNC71_001523"
     CDS             complement(21066..22829)
                     /locus_tag="LNC71_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466598.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(22832..24547)
                     /locus_tag="LNC71_001524"
     CDS             complement(22832..24547)
                     /locus_tag="LNC71_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466599.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(25326..25550)
                     /locus_tag="LNC71_001525"
     CDS             complement(25326..25550)
                     /locus_tag="LNC71_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466600.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         8337 a         4603 c         5177 g         7530 t
ORIGIN      
        1 taattggttt tttttaaagc aaaagataat ctatatcatg atgaacaaac agagtttttt
       61 cctccaattg atggctttcc tgatctttcc tattttgaat aaagtgtttc tgtataattt
      121 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
      181 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
      241 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
      301 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
      361 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
      421 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
      481 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
      541 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
      601 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
      661 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
      721 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
      781 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
      841 gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa ttccaactta tttccgaaga
      901 agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa gtcgcttagt ttcgagtagt
      961 aagaaaaatt ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag
     1021 aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag
     1081 tttctttttg tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat
     1141 tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa
     1201 ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat
     1261 tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa
     1321 attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat
     1381 gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa
     1441 aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta
     1501 cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg
     1561 aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg
     1621 cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt
     1681 caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc
     1741 cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta
     1801 cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag
     1861 aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt
     1921 tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata
     1981 aagctgttta taatggacca ttcacattag aagattttga tggaccagga acagatacag
     2041 aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa
     2101 tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag
     2161 cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata
     2221 cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac
     2281 cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag
     2341 taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg
     2401 taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg
     2461 acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt
     2521 tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc
     2581 aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct
     2641 ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg
     2701 ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc
     2761 gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg
     2821 ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg
     2881 atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca
     2941 aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag
     3001 aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc
     3061 gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt
     3121 agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag
     3181 gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa
     3241 tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat
     3301 gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt
     3361 tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc
     3421 atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga
     3481 agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt
     3541 ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt
     3601 gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct
     3661 aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa
     3721 tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt
     3781 gttagctgga agaatcggac catccttgca gttagggtta caggcaatta tctttggaac
     3841 agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac
     3901 atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt
     3961 attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt
     4021 tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg
     4081 gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc
     4141 aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc
     4201 attgatgact ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga
     4261 aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga
     4321 ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt
     4381 gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg
     4441 aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga
     4501 atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt
     4561 gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat
     4621 ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga
     4681 tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat
     4741 cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc
     4801 aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt
     4861 aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact
     4921 agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga
     4981 taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat
     5041 tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac
     5101 aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga
     5161 atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat
     5221 tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat
     5281 tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt
     5341 aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat
     5401 tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg
     5461 tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa
     5521 gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat
     5581 tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca
     5641 gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa
     5701 atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt
     5761 ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc
     5821 attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga
     5881 caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc
     5941 aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca
     6001 ctggtatgtt atccggaaat actgattgct gatgaaccta caacagcgct tgatgtgact
     6061 attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt
     6121 atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg
     6181 tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat
     6241 ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg
     6301 tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat
     6361 ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta
     6421 tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg
     6481 ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt
     6541 caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt
     6601 caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga
     6661 aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac
     6721 gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac
     6781 gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca
     6841 agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat
     6901 tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt
     6961 gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg
     7021 gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg
     7081 tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca
     7141 agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt
     7201 taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac
     7261 aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat
     7321 tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc
     7381 agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac
     7441 tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt
     7501 tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa
     7561 gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg
     7621 ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc
     7681 gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat
     7741 gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca
     7801 aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg
     7861 atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat
     7921 cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa
     7981 gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa
     8041 tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta
     8101 actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat
     8161 gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag
     8221 aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat
     8281 cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt
     8341 ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa
     8401 ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca
     8461 gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt
     8521 atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa
     8581 aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg
     8641 acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt
     8701 ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa
     8761 gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat
     8821 tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt
     8881 tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt
     8941 ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct
     9001 ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat
     9061 gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca
     9121 gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt gttactatga
     9181 cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct
     9241 ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta
     9301 ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt
     9361 gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat
     9421 gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct
     9481 tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct
     9541 ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa
     9601 tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta
     9661 aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa
     9721 atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa
     9781 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
     9841 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
     9901 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
     9961 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
    10021 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
    10081 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
    10141 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
    10201 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
    10261 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
    10321 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
    10381 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
    10441 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
    10501 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
    10561 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
    10621 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
    10681 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
    10741 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
    10801 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
    10861 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
    10921 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
    10981 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
    11041 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
    11101 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
    11161 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
    11221 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
    11281 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
    11341 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
    11401 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
    11461 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
    11521 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
    11581 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
    11641 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
    11701 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
    11761 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
    11821 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
    11881 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
    11941 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
    12001 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
    12061 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
    12121 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
    12181 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
    12241 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
    12301 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
    12361 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
    12421 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
    12481 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
    12541 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
    12601 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
    12661 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
    12721 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
    12781 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
    12841 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
    12901 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
    12961 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
    13021 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
    13081 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
    13141 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
    13201 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
    13261 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
    13321 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
    13381 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
    13441 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
    13501 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
    13561 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
    13621 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
    13681 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
    13741 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
    13801 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
    13861 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
    13921 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
    13981 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
    14041 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
    14101 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
    14161 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
    14221 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
    14281 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
    14341 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
    14401 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
    14461 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
    14521 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
    14581 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
    14641 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
    14701 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
    14761 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
    14821 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
    14881 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
    14941 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
    15001 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
    15061 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
    15121 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
    15181 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
    15241 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
    15301 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
    15361 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
    15421 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
    15481 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
    15541 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
    15601 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
    15661 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
    15721 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
    15781 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
    15841 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
    15901 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
    15961 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
    16021 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
    16081 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
    16141 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
    16201 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
    16261 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
    16321 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
    16381 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
    16441 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
    16501 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
    16561 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
    16621 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
    16681 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
    16741 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
    16801 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
    16861 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
    16921 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
    16981 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
    17041 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
    17101 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
    17161 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
    17221 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
    17281 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca
    17341 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
    17401 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
    17461 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
    17521 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
    17581 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
    17641 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
    17701 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
    17761 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
    17821 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
    17881 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct
    17941 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
    18001 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
    18061 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
    18121 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
    18181 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
    18241 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
    18301 tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga
    18361 tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg
    18421 ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct
    18481 gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt
    18541 tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc
    18601 ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc
    18661 actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc
    18721 aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat
    18781 aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc
    18841 aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa
    18901 tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt
    18961 aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc
    19021 tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc
    19081 ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg
    19141 ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt
    19201 gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt
    19261 catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc
    19321 cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc
    19381 cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa
    19441 ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata
    19501 ttttttatat acgtttttca gagccatttc taccataata cgttccacac tttctttttt
    19561 agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga
    19621 acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata
    19681 agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc
    19741 tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc
    19801 aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca
    19861 gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg
    19921 ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg
    19981 agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga
    20041 tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact
    20101 ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc
    20161 attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa
    20221 aagctaaaca aactgataat atgcgatata atacgaacgt attggataat ataaacaata
    20281 atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag
    20341 atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg
    20401 cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga
    20461 tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga
    20521 ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg
    20581 agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt
    20641 ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct
    20701 tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct
    20761 acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta
    20821 gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg
    20881 tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac
    20941 cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa
    21001 gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct
    21061 tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga
    21121 agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg
    21181 tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc
    21241 attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct
    21301 tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg
    21361 cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc
    21421 aaacgttcta caaattcata catataagag gcttttaggg catcattgat ttctgcgtca
    21481 cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta
    21541 tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca
    21601 tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca
    21661 agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca
    21721 gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca
    21781 aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc
    21841 ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc
    21901 agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc
    21961 gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg
    22021 ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc
    22081 aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca
    22141 aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt
    22201 cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt
    22261 tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg
    22321 accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc
    22381 actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca
    22441 tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat
    22501 cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga
    22561 ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat
    22621 agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg
    22681 atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt
    22741 atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc
    22801 gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt
    22861 cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac
    22921 cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa
    22981 taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat
    23041 aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc
    23101 ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt
    23161 ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag
    23221 cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag
    23281 tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca
    23341 gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg acatcataga
    23401 aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa
    23461 ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag
    23521 tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag
    23581 tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc acccgatttc
    23641 ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa
    23701 gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat
    23761 ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg
    23821 tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat
    23881 ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac
    23941 gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt
    24001 tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca
    24061 ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc
    24121 cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa
    24181 gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact
    24241 cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc
    24301 cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag
    24361 acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc
    24421 cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa
    24481 atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact
    24541 gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac
    24601 attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt
    24661 attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa
    24721 aacatgccag tctcttcatt cataggaacg tctattttac ttaatttttc tataaatgaa
    24781 ttctgcataa aaaatatatc aagtaaatgt ttatagatga aactctattt acttcttttt
    24841 taatttgttc ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa
    24901 atctaattga ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt
    24961 gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta
    25021 tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt
    25081 gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt
    25141 aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag
    25201 tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt
    25261 tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt
    25321 gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa
    25381 aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata
    25441 gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga
    25501 aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca
    25561 tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg
    25621 ttttttagaa taaaaattat aagggat
//
LOCUS       DAFCHQ010000036        25627 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000036 DAFCHQ010000000
VERSION     DAFCHQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.036"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..601
                     /locus_tag="LNC71_001526"
     CDS             47..601
                     /locus_tag="LNC71_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6466601.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(774..1247)
                     /locus_tag="LNC71_001527"
     CDS             complement(774..1247)
                     /locus_tag="LNC71_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6466602.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1259..1795
                     /locus_tag="LNC71_001528"
     CDS             1259..1795
                     /locus_tag="LNC71_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6466603.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1835..2206
                     /locus_tag="LNC71_001529"
     CDS             1835..2206
                     /locus_tag="LNC71_001529"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6466604.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2490..3761
                     /locus_tag="LNC71_001530"
     CDS             2490..3761
                     /locus_tag="LNC71_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBK6466605.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4006..5031
                     /locus_tag="LNC71_001531"
     CDS             4006..5031
                     /locus_tag="LNC71_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6466606.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5325..6434
                     /locus_tag="LNC71_001532"
     CDS             5325..6434
                     /locus_tag="LNC71_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK6466607.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6517..7413
                     /locus_tag="LNC71_001533"
                     /pseudo
     CDS             6517..7413
                     /locus_tag="LNC71_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7429..8253
                     /locus_tag="LNC71_001534"
     CDS             7429..8253
                     /locus_tag="LNC71_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6466608.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8285..9595
                     /locus_tag="LNC71_001535"
     CDS             8285..9595
                     /locus_tag="LNC71_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HBK6466609.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9743..11002
                     /locus_tag="LNC71_001536"
     CDS             9743..11002
                     /locus_tag="LNC71_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6466610.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11012..11884
                     /locus_tag="LNC71_001537"
     CDS             11012..11884
                     /locus_tag="LNC71_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6466611.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11896..12726
                     /locus_tag="LNC71_001538"
     CDS             11896..12726
                     /locus_tag="LNC71_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6466612.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12766..14949
                     /locus_tag="LNC71_001539"
     CDS             12766..14949
                     /locus_tag="LNC71_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK6466613.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15010..16230
                     /locus_tag="LNC71_001540"
     CDS             15010..16230
                     /locus_tag="LNC71_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBK6466614.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16227..17687
                     /gene="gtfA"
                     /locus_tag="LNC71_001541"
     CDS             16227..17687
                     /gene="gtfA"
                     /locus_tag="LNC71_001541"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBK6466615.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17764..18870
                     /locus_tag="LNC71_001542"
                     /pseudo
     CDS             17764..18870
                     /locus_tag="LNC71_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19192..19401
                     /locus_tag="LNC71_001543"
     CDS             19192..19401
                     /locus_tag="LNC71_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK6466616.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19394..19543
                     /locus_tag="LNC71_001544"
     CDS             19394..19543
                     /locus_tag="LNC71_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBK6466617.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19697..20362
                     /locus_tag="LNC71_001545"
     CDS             19697..20362
                     /locus_tag="LNC71_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6466618.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20646..21731)
                     /gene="xerS"
                     /locus_tag="LNC71_001546"
     CDS             complement(20646..21731)
                     /gene="xerS"
                     /locus_tag="LNC71_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBK6466619.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22065..22298
                     /locus_tag="LNC71_001547"
     CDS             22065..22298
                     /locus_tag="LNC71_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBK6466620.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22295..22702
                     /locus_tag="LNC71_001548"
     CDS             22295..22702
                     /locus_tag="LNC71_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK6466621.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23839..24357)
                     /locus_tag="LNC71_001549"
     CDS             complement(23839..24357)
                     /locus_tag="LNC71_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBK6466622.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25475..>25627
                     /locus_tag="LNC71_001550"
     CDS             25475..>25627
                     /locus_tag="LNC71_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6466623.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         8729 a         3924 c         4789 g         8185 t
ORIGIN      
        1 tatacataat cattaactca gaatattttt aagaagggaa acaatagtga gctataatgt
       61 acaaccgtta aggacaaatg aagagataca agattttcag ttttggctaa gaagaacaag
      121 taatccggaa agagatagtt ttttattttt gtttggaatc aataatgggt tacgtatgtc
      181 agatattata ggattaaaag ttggagatat acgaggcaaa agtaagccta ttattgttga
      241 acataaaaca ggtaagcgaa aaccgatttt tatagataat ttgagagaag aaattttatt
      301 atatactgaa ggtaaagaag aaaatgattg gttatttccg agtcggcaac aaggtcgaca
      361 tataacaagg gatcgtgtct atcagatata tgcagatata gcagaaaaat taggacgaga
      421 tgatatagga acacatacat taagaaaaac ttttgggtat cactactaca agaaaactag
      481 agacattgct accttaatgt ttatttttaa tcatagctcg caagcaatta caaaaaggta
      541 cattggtata actgaagatg aaataggagc atcacttcgt ggatttaaac taggtgtata
      601 atttaaaaat ttttataaac agtaaagaca aagattaaaa gctattttta tctagaacga
      661 ttttatttca taaagttcgc ggaataaaag ctggggttac tggtacttgg tataggttcg
      721 tgttatataa actaagtaat taaataacat ttttaggttg gaaaaggatg aaattaaaaa
      781 aagagaatca atacggtctc aaaaacgcaa acgctgcgga ataagaaaaa tactgataac
      841 tcaacgttta gcagactatc taatagggat agtctgcttt tttcgttttt gaccatactt
      901 ttgaccttct gcaaacttcc ttgcttttcc tctgtagaac tcatgaaatc tgcaaaacga
      961 attgccgttt cctctgtctg ctttttcgcg atatgggtgt atatatcatc catagtggtt
     1021 ttaatatctg tatgtccaag tcgaacatac acttctttga cagacgcacc agattcaaag
     1081 agcagactac aatgggtatg acgaaaacca tgaagtgtga tatgttttaa gttattctct
     1141 ttaatgatga ttttcaaatt gtgatttaca tagtctaaat aaaggggctt attttcttct
     1201 gatgaaaaca aaagttgttc accatctttt ataggtttga ttctcatttt tagatattat
     1261 gcccgcacgt attcatcaca tttctattgt gaatcgcttt attcgaccga catttgactt
     1321 ttatcataat atactcggtt taaaattact aatgaaaaca attaatcaag acgatcatac
     1381 catgtatcac ctgttttttt cagataatca ccaccgtgtg ggaactgaac tgactttttt
     1441 tgaagtacaa gaagggaaca accagttttt tggaaccaat acgattgaac gaacgatact
     1501 aaaagttcct tccgaagcat cattgcattt ttgggaaata tattttgaaa cacaaggcgt
     1561 ttgccattat ggaatcgaat cattttctgg acgacctatc ttacgttttg aagggccaga
     1621 tgcaacacga ttagctttag ttcccttacg cgaatttgaa gatattgatg attatttccc
     1681 gtatgtcaca gaccagatcc ctacagagca tgccatttta ggaattgatg ccattcaact
     1741 acgcgtacaa tattccgatg cgactgaacg agagttattg tcggtaggct ggtagcacaa
     1801 agaatacgtc agtttctttt cgggtcatca acgagtgaca atcttagaaa atcaagacac
     1861 tcagttctat caagaagttc agattattca agatcgtgag aatccagttg ccattgaagg
     1921 gattggtagc gtccatcatg ttgcgtttgg cgtagaaaat aaatctgatt tacaacgcat
     1981 cgacaaacaa ttacaagagc gcaattttat aaattcagga ataaaagatc gtgaattttt
     2041 tgtatcactt tattatcgtg acccaaatca attattgatt gaaattgcta caggggaagg
     2101 aaacctagat gcaaaagcgt atgaaaatca aagtccacgc tttgaagaaa ttcctttatt
     2161 tttaccgcag tacttaaatt tcattcgtga acaagtggag caataattag ctaatcaaat
     2221 ccatgacgaa gagtaagagg ttgactcata agtcaaaaaa taaagaaaaa cgcatcaaat
     2281 ccattctaaa gaatgttgat ttgatgcggt tcctattttt ataaactatc attttcgact
     2341 aataaaacta cttatgagtc agcctctctt tttttcttga tttttatacc tctttctagg
     2401 taaactaaac atctgccaaa aataccgtgt ctcattgaaa cattctcaac tatttcaaaa
     2461 taaaggggag taccaaatga acgagtatat tgaaattatc cacgcaagag aaacaactta
     2521 aaaatgtcag tctcaaaatt cccaaaaata aaattagtat ttttaccagc gtatctggtt
     2581 caggaaaatc atcgattgtt tttgatacta ttacccaaga agctggtcgt caactgaatg
     2641 acacctatga tagttttacc cgtttatttt tacctaaata cagtcgtcca gaggttgatg
     2701 agattgataa tctttctacg gcgattgtgg tcaatcaaaa gaagattggt ggcagtgcgc
     2761 gctcaacgtt agggacgatt actaacatca atgcgttatt tcgtgtgttg ttttcatgtt
     2821 ttgcaacacc tagtttagga tatgcaaatg cttattcatt taatgatcct agtggaatgt
     2881 gttctgtctg tcagggaatc ggtaagacga ttgcattttt aataggattg ctttattttt
     2941 cggctattga taatgctgat tttgtatttt ctgatccatt taccgcagaa gttttgtctg
     3001 gaattgagga acaggcaaag aatcatggtt attttgtatt ggtgcataat gttaattcag
     3061 cagaagatgt taaaactatt cagagaaatt ggcgttttga ggggttcatt gttgttggtg
     3121 tacgtgtaaa agaatttttg gaattaaata acgtttttga tggtcctgtt gtttatattg
     3181 atacgcatct ttcaaaagag acattaagag aacttgaaca acaaatgaat cgtttatttg
     3241 taaatacaaa tgattaccaa gctagtacat tggcagtgga acatttgatt cattatggtc
     3301 atgagaaaat agcgtttctt tcttttgact acattgtagg tgaaccaagt gttattcaag
     3361 aacgacatca agcttatatc gatacattaa agaaatatca tttaaccttt aaatcgcatt
     3421 ggatatataa caactctgaa tttgagcgaa tttatcaaga attagctgaa tttactgcaa
     3481 ttgttgttac tggagattat ttagcggcta aattagtaaa atttttaaaa aataagaata
     3541 aacgagtgat ggaacaatta tcgattattg gatttgatga tattactttt gcagaattaa
     3601 tggatcctgc gttgacaaca atacgactaa atccaggtaa caagggggcg attgcttttc
     3661 accaactgat tggactggcc aaacaagaag aaataaaaaa tcaagtacta ctattagagg
     3721 gagaattgat ttctcgagac tctgttcaca aaatcaaata aaaaagctct gtgaaataag
     3781 tatagttaac aataaaatat ccgaacgata gagagaatga actgtgatct agcagttcct
     3841 cgtcgcaaat tcactagaat tagcattatt tactctaaat tgttcggata ttttttcttg
     3901 tttttaatta tgattacagt gcctattttt aacgaaattt gcttattttc aaaatgtatg
     3961 attatatgtt agtaaacact ttcaaatatg aaaagaggag ctattatggt aggaattagg
     4021 gatgttgcaa aacgggcggg tgtgtccatt gcaacagtat cgcgcgtgct aaatgaggat
     4081 ccgacacttt cagtaaccga tgaaaccaaa cgaaagattg cagaatcggt taatttttat
     4141 cattatgtca aaaaaagtcc cgttgcaaaa aatttaacaa atattgcatt aataacgaca
     4201 gtttctgaag tagatgagtt ggaagatccc tattttcgaa cgattcgcag agggattcaa
     4261 gtcgaagcag aacaaagcaa agtcaatttg aagaaaatta ttcgtttgtc agaaacaccg
     4321 ttggagcaag acgatttgct aaaatgtcaa ggtgtgttga ttattgggca ggtgctatct
     4381 tctgtgattg aagaagtatc agcaattaat tcgaatgtcg tagtgattga tgaccctagt
     4441 attgaagctg aagtagatgc agtttatact gatttaacaa aagcaacgaa tcagcattta
     4501 gatcgattgt ataaaaaagg acaccgaaac attgcgttta tcggaggagg acgtgttcgt
     4561 ttagatagcc aaggaaataa acacgtgtca gatgatgatt atcgaaaaat agcctatgag
     4621 acgtggatga aacaaaaagg attagaaaaa tatagtcaaa tttatttaga aggctggacc
     4681 acacttgacg gaatgcaggc agcagattta cttttgaaaa aacatgctaa agataagttg
     4741 ccgactgcta tcatggtagg aaatgatcct attgctgtgg gggtttatcg ttcacttcaa
     4801 aaaaatggtc tacatattcc agaagatatt tcgattgtga gttttgataa tattgaagtt
     4861 gctgaatttt taacacctgc attaaatacg gtgaatgtga atacagaaga aattggaaaa
     4921 ctagcagttc gcatggttaa tgaacgaata aaagaagtta gaaatgtttc gatcaaagtg
     4981 attgtttcta ataatattat tgttagagaa agtgaaaaaa atatcgatta aaaaagtaaa
     5041 catccaaaaa agggtgttta cttttttgtt tactaatatt tttagtaaaa aatctcgaca
     5101 aaacattaag taaacagtta tggtgttgtt aacaaaaaga aaacgcattc aaaacaggag
     5161 gaattaagat gaaacatttc aaaaaaatag ctttaacagg gatagctgtt ttaactggga
     5221 cattactcgc agcatgtggt gggggagata gtaattctaa agacgggcaa gtaactttaa
     5281 gctatgcgag ttgagatact gaccaagctc caggactaag aaaaatgtta gatgaatttg
     5341 aaaaacaaaa tcctgatatc aaagtagaaa tggagacaac gccttgggat caatattggg
     5401 ttaaattaga agctgcaaca acgggaggta atatgccaga tgtagtaacc atgcactcta
     5461 gcgaatccta taaatatatg tcacagaatg ctttgatggg acttgatgat gttatcaaag
     5521 aaaatgatat tgacatggga aactttactg aaggaattgc tgatttctat acgcttgaag
     5581 acgaactata cgcaatccct aaagatgctt ctgtagtggg gctatggtac aacaaagaat
     5641 tatttgataa tgcaggtgtc gattatccag atgaaacatg gacatgggaa acactcagac
     5701 aagctgcaat tgatttaaca gatgaatcaa aaggcatcta tggttttgca tcacatattg
     5761 gaacagagac tggctactgg ccatttattt atcaaaatgg gggagaagta tttgccgatg
     5821 acaatacaaa atcaggtttg aataccccag aagcacaaga agctttagct ttttatcgag
     5881 atattgcact tgttgatcat gcttcaccaa caattacaga attacaagat cctgataagt
     5941 taagtcgttt tcaatcaggt agattagcaa tgatgattga aggaaactgg tttgcaccgg
     6001 gatttttgaa aaatgactat ctagttgaaa atgttgcttc tgccgtgtta cctaagggaa
     6061 aagttaatgc aacaattaca aatggacttg gttgggctgc cagtgcgagt acaaaatacc
     6121 cagaagaagt gaaaaaacta atggcatttt tagctagtga ggacgccaat aaaatccaag
     6181 cggaaacggg ggcatcaatc ccagcactaa aaggctttga agacgagtgg gctgcttctt
     6241 cagatacaat tgatttgagt gcttttggaa aaatgttgga gtatggagtc cctcgtccat
     6301 acaataaaga tggattacga gctgaacaag cagaagttca aattattaac cgtattctgt
     6361 ctggtgaagt aactgttgaa gatggaaccg ctcaaattga aaaagaagtc aatgcgatat
     6421 tgagcggaaa ttaaattagt taattataca atatacaggc gaaaggattc tatttcgtct
     6481 gtatattaat ggatgaatac caaaaagggg gatattatga aaaatcgaat ttttggaaaa
     6541 tatcgtaaag atcaagtgat atggggctat atttttattt ttccaacggc attaggatta
     6601 attttattaa atatatggcc agcaattcaa acagcaattt taagttttca aaaaacggta
     6661 gggttcggtg atactgaatg ggtcggatta agaaattacc aaaaaatgtt tgcagacggt
     6721 gaagtttttc agtctttatt aaacacatta atttatgcag tggtttcgat acctagtatt
     6781 gttgtattat ccttgttggt ggcagtttta atgaatagaa aagttaaagg attgtcgatt
     6841 tatcggacaa tttattttct accagtcgtt gctgcaccat ccgcagtcgc aatgatttgg
     6901 cgctggatgt tcaataatga ttatggactg attaataaca tgttggctaa aataggtctc
     6961 gatggaccag catggttaac ggatccatcg attgcgattt attctattat cattgtagga
     7021 gtttggagtg ctattggtta taacatggtg ttattactag ccggcttata agaaattcct
     7081 aaagattatt atgaagcagc ggaaattgat ggtgctagtc caatccagca gtttttcaac
     7141 attacgttac cattagttac accaacatta ttttttgtag ttgtaacaac agttatcaat
     7201 gctttccaag tatttgatgt tatttttatg atgattactc ctaatagtac cgccatgttt
     7261 aaatcacaat cattagccta tttattctat aaacattcat ttattttaaa tgacaaggga
     7321 tatggttcgg caatcgttat gttcctttta gtgattatat taattattac tgcaattcaa
     7381 atgaagttgc agaaaaagtg ggtaaactat taaggaggct atcaaattat gaaaaataaa
     7441 tttttttcaa atgcgccaat ccatatcatg cttattttag gtggtattgc aatggcatta
     7501 ccttttattt ggatgatttt aacttcaggg aaaacgttgg ttgaatctac ccaaattcca
     7561 cctacaattt tccctgagaa atttcagcta agtaactatc aacaagtact tgatttatta
     7621 ccattcatgg ctttttatat caatacattt ttaatggttg ttggacgagt gattggctcg
     7681 gttttatttt cagcaatggc tgcatatgct tgtgcgcgtc taagttttcc agggaaaaat
     7741 atccttttca gcttagtact tattcaaatg atgattcccg gttccatttt tattactcca
     7801 cagtatttat tagcacaaaa attgggtatt ttaaatacaa ttccagcatt aatattacca
     7861 ggggtagtta gtgcgtttgg tacgttttta ttaagacaat ttttcttagg tttacctgat
     7921 gaattggagg aagcggccta tttagaaggt gccaatattt ggacgatctt tttcaaaatt
     7981 atgttgccgc ttgctaaatc aggattggtt gcacttggaa tattcacagc gttgtttgca
     8041 tttaaagatt taatgtggcc attgattgtc aatacttcaa ttgataagat gcctttagct
     8101 ccgggattag caaacttaca aggacaatat gccacgaact tcccacaatt aatggcaggt
     8161 tcgttattag caatttggcc aatagttgtg atattcatcg ttttccaaag aaaattcatt
     8221 gaaggaattg ctacaacagg tagtaaatta taaaactatt aattagtgat tggagagtaa
     8281 gtacatgaca aaaattacat ttattggtgc aggaagtact atttttgcaa aaaacgtatt
     8341 aggcgatgcg atgttaactc caagtctaca agatgcacat attgcattgt acgatattga
     8401 tgaaaaacga ctaaaagaat caaatttaat gcttaaaaat attaataaaa attcgaatga
     8461 aaatcgtgct gatattgttt cttattatga tcgcaaagaa gcgttaagag aggctgattt
     8521 tgttatcaat gctattcaag tgggtggtta taaaccttca acagtgattg attttgatat
     8581 tcctaaaaaa tatgggttgc gacaaactat tggtgacacc atggggattg gtgggatttt
     8641 tagaacccta cggacactac cagtaatgtt agactttgct aaagaaatgg aagaagtttg
     8701 tccaaatgct tggttattga actatacaaa tccgatggcg atgttaacca tgggtgtttt
     8761 aaaagcgacg agtattaaga ctgttggttt atgtcatagc gttcaagtat gcgtaccaga
     8821 attgtttgag catttaggaa tagccgatca atatgactta aatgactttc aatggaaaat
     8881 tgcaggcatt aatcatatgg cattcttgtt ggaaattaca aaaaatgggg aagatttcta
     8941 tccaacaatt cgtcagttag ctgcagaaaa accaaatcca catccagatt ctgtacgttt
     9001 tgaattgttg aaacgttttg gttactatat tacagaatca agtgaacaca atgcagaata
     9061 ccatccatac tttattaaat caaaataccc agaattgatt gacgaattag ggattccgtt
     9121 ggatgaatac ttacgtcgtt gtgaacaaca aattaatgat tgggaaagca tgcgagagga
     9181 cattgtaaat aatggggagt taacacatac gagaagccgt gaatatgctt catatatcat
     9241 ggattctatt acgacaggaa ctccgtcagt gattgcagga aatgtcttaa ataaaggctt
     9301 aatcacaaac ttaccgacag atgtttgtgt ggaagtacct tgtttagtcg ataaaaatgg
     9361 cgtgcaacca acctatgtcg gtgaattgcc aacacaatta gctgctttaa atcggacgaa
     9421 tatcaatgtt caagaactga cagtagaagc cgctttaaca ctagataaat caaaaattta
     9481 tcaagcagcg tatttagatc cacatctatc atctgaattg tctttaacag atatcaaaaa
     9541 tttagtggat gatttaatcg aagcacatgg ggactattta cctgaatata attaaaaaga
     9601 agctgaaaga gtaaggaatt ttcaattcct tactcttttc ttagttaaat ttagtaaaaa
     9661 tattgacaaa acacttagta aacagttaca ttagtgttaa caaaaagaaa acgcattcaa
     9721 attgtataag gaggagcata aaatgaagtg gtctaaaaaa attattttag cttgtatagc
     9781 actaggagca acgatctctt tgactgcttg tggaaaaaat caggaagatg caagcggtaa
     9841 ggtagaaata gagtttttta atcaaaaaaa agaaatgact caaacgattc aggagattgc
     9901 taaagatttt gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg taccgaatgc
     9961 tggtgaagta attaaaacac gtatgcttgc aggcgatgta ccagatgtca ttaatcttta
    10021 tcctcaaagt atagaattga aagaatgggc gaaagctggt tatttagagg acttaaccga
    10081 agaaccgtat ttagagaata tcaaaaatgg atatgcacaa cgttttgcta ttgaggatcg
    10141 tgtatatagc ataccattaa ccgctaatgt ctatggtttt tactataaca aaactgcttt
    10201 tgaggaaatg gggattcaag caccagaaac ttgggcagaa tttgaaaaaa tagtagctga
    10261 gattaaagat caaaacaaag taccgtttgc gattgcagga gcagaaggct ggactttaaa
    10321 tggttatcac caactagcta tagcgactgc tgctggtggt gaagaggaag cgaacaatgt
    10381 ttggcgtttc tcagaagtta atggaattaa tgctgaaagc aaagaaatgc aaaaagattt
    10441 taatcgatta gatttgttaa gagaaccaaa agctttgcaa aacaactggc aaggtgccgg
    10501 ttataacgat acagtagtta cttttacaaa aggtgatgcg ttgattatgc cgaatggttc
    10561 atgggcaatg ccgatgattc attcacaaaa tccagatttt gaagtaggaa cttttccttt
    10621 tccagcagat gaagcaggtc aaagtctaac gattggcgca ggagacctag ccttatcagt
    10681 ttctgcaact tctcaacata aagaagaagc caaacgtttt gtagaatata tgacaacacc
    10741 tgaagcgatg cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg agggcgttat
    10801 cgagaatact caggattcac cattaagtgg tttaactgaa cttgctttta ctgatcgtca
    10861 tttagtttgg ttagccaaag attggaatag tgaaaatgat ttttatacct taacaacaaa
    10921 ttatttacac aatggcaatc aaaaagccat gattaacgca ttaaatgctt tctttaatcc
    10981 aatgaaagct gacggaaggt aggaaaataa aatgaaaata aaagaaattt taaatcgtta
    11041 ctgggcaatc atttttgtag caattccaat tattttgcag atgatttttt tctactttcc
    11101 aatggttcaa ggtgcttttt acagtttaac taattggact ggtctgactt ataattttga
    11161 ttttgtgggt ttgaacaatt ataagattct tttttctgat ccgaagttta tcaaatcgat
    11221 tggcttcact ttaattgtga cgctttgcat gattgctgga caaattggtt ttggtatttt
    11281 gattgcgcgg gcgttgaacg caaatattaa agggaaaaca ttctttagag catggttctt
    11341 ttttccagcg gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa tttttaacta
    11401 tggtttacca aaaattggta gtatgttagg cattgaattt ttacaaacca gtcttttagg
    11461 aacaacatgg ggagcgattt ttgcagtaat ttttgtttta ctttggcaag gaattgccat
    11521 gccagtcatc attttcttag cgggttttca aagtattcca caagaaatta ttgaagctgc
    11581 tagaatggat ggggcgaatg ataagcaaat tttttggaat attgaaatca cctacttatt
    11641 accaagtatt tcaatggtat ttattatggc attaaaaggt ggcttaacag catttgatca
    11701 aatttttgct ttaactggtg gtggaccaag cgatgccaca acatctttgg ggttacttgt
    11761 ttataattat gcttttaaaa acaatcagtt tggttatgcg aatgctattg ctttagtctt
    11821 atttatctta attgggattg tttcactggc acaattaaaa ttatcgaaaa aatttgaaat
    11881 ctaggaggtt tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata cttgctttta
    11941 ttattagggg gcttcatgat tttagttccc ttagtttcaa cgctattcgg ttcatttaaa
    12001 acaacacccg atattatgaa taatttcttt cgttttccta atccagcgac tttggaaaat
    12061 ttccgaagat tgttagcgga tggaattggt cattactttt ggaattcggc aatcatcaca
    12121 atcattgcag tagttgctgt gacagcaatt attcctatgg ctgcttattc actagcaaga
    12181 aaaatgactc gtgggaaagc atacggattg atgtatttat ttttaatttt aggaattttt
    12241 gttcctttcc aagtaattat gattccgatt accgttatga tgagtaaaat tggtttagca
    12301 aatatgtggg gattaatctt actctatctc acttatgccg taccacagac actattttta
    12361 tatgttggtt ttattaaact aaatgtgcct atcagtctgg atgaggctgc tgaaattgat
    12421 ggggctagtc actttacaac ttatttcaaa attgttttcc caatgttaaa accaatgcat
    12481 gcgacgacta ttattcttaa tgctctatgg ttttggaatg atttcatgct accattatta
    12541 attttaaatc gtgattctaa tatgtggaca ctaccattgt ttcaatataa ttatacggga
    12601 caatatttta acgactatgg tccaagtttt gcttcctata cagtaggaat tgtcacgatt
    12661 acactagttt atcttgcttt ccaaagaagt attatagctg gaatgagcaa tggagcagtg
    12721 aaataaccaa taaattattc tcattataaa ggagttcata aacttatgat tatatttgac
    12781 aatcacacac aaacatttca tttacaaaat aaaaaaatta gctatctatt aggaatcgaa
    12841 gaaaataatt atttaacgca tctttatttt ggaaaaaaga ttacgcacta ttctggtggg
    12901 tatcgctacc cccgaacaga tcgttccttc tcacccaaca atgcaaatgc gcaagatcgt
    12961 ttattatctt tagacacatt accattagaa tttccaggat atggacatgg tgattttaga
    13021 gaaccggcgc ataatattaa attaggaaac ggtagtcgtg taaatgattt tcgttatgat
    13081 agctacgaaa ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt atatacggaa
    13141 gatgatgatg aagctgaaac gctgattatt caattgaaag acgcgattag tggattgact
    13201 ttacagttgt tctacacaat ttatcaaaat cacagtgtaa ttattcgttc tacgcaatta
    13261 gtaaataata gtgatgaaac agtggagatt aaccgcttag ctagtcaatc aattgatttt
    13321 ccaaatcgtc cactcgagtt aattcattta aatggtacct gggcaagaga acgtcaaatg
    13381 actagagaaa aaattcatac agggataaaa gtattagata gtaaacgcgg atcaagcagc
    13441 catcaacaaa atccgtttgt tgctttagta gatgccaaaa cagatgaatt tcaaggagag
    13501 gcatatggat ttaatttagt ttatagcggt aatcacgaaa cggtgattca aaaagatcca
    13561 tttgaccaaa cacgggtcat tatgggaatt aattcattta attttggttg gcaactaaaa
    13621 actggcgact catttcaaac gccagaagtc gtgatggttt atagtaatga aggtctgaat
    13681 acgttatcac agacgtatca tgaattgtat aatcaccgtt taattcgtgg aaactatcgt
    13741 ttaaaagaac gtcccacgtt aatcaacaac tgggaagcaa cgtattttga ttttaacgaa
    13801 gaaaaaatca tgaatattgt cgatgatgcg aagaacttgg gcattgaaat gtttgtttta
    13861 gatgatggtt ggtttggcgg tcgtgaaaat gacttcactt cattaggtga ttgggttgaa
    13921 acagaaggca aacttgcttg tggtttggaa aatttagcca agcaagtgca tgataacggg
    13981 atgaaatttg ggatttggtt tgaaccggaa atgatttctg aagatagtga actatttcga
    14041 gcacatccag attgggcatt gggtattcca ggtcgtgacc gctcaattag tcgaaatcaa
    14101 tatgttcttg atttttcaag acaagatgtt cgtgataata tctatcaaca aatgacggcg
    14161 atttttgatc gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa tatgacagaa
    14221 gtctattctg tcttgttgga ctcaacgatg caaggtgaag tggctcatcg ttatatgtta
    14281 gggttatatg aatttttaga aaagttaaca acgaattatc caactatttt atttgaaagt
    14341 tgttcgggtg gaggcggtcg ttttgatgca ggtttcttgt attacatgcc acaaacttgg
    14401 acgagcgaca ataccgatgc cgtggctcgt ctgaaaattc aatatggaac cagcttagtg
    14461 tatccaattt caagtatggg tgcgcatgtt tcagcagtcc caaatcatca aacaggaaga
    14521 gaaacgagtt taactattcg tggcaatgct gcgatggctg gtgtttttgg ttatgagcta
    14581 gatctaacgc aattaacttc tgaagaaaaa gcagaaatta aagaacaagt tgccttttat
    14641 aaaaagcatc gccaactttt gcaatatgga actttttatc gcttggaaag tccgtttgaa
    14701 ggcaatgata ctgcatggat gtttgtctca aaagaccaaa cagaagccat ggtcttttat
    14761 ttccgagtat tagcagaagc tgcgcatcca ttagtcacat tgaaattagc aggattagca
    14821 gaagaagcaa cttatcaaat ggaagatgta acatttgctg gcgatgaatt gatgaatctc
    14881 ggtttttata ttgactcaga actacatggt gattatgcga cacaacgctt ttatttgaaa
    14941 cagatatagt agcttgaatt tacaatataa gtgcaacacc aaaagagact cattccaaac
    15001 gaagaaaata tgttttattt acacgcattt actaaaaagc agcctgattt gaattgggaa
    15061 aatgaagaag tacgtgaaga aatctatgca atgattaact attggttaga taaaggacta
    15121 ggcggattta gaatcgatgc gatattaaac atcaaaaaaa aactagagta tggcatcttt
    15181 gaatctgatg gagaagatgg gttagccttc attgggccct ggattcttaa tcagtcgggc
    15241 attgaagtgt ggttaaaaga aatgagagag cggacattta aaccacataa tagtatgacg
    15301 gtagcagaag ccgatgttcc taatgaacgt ttagatgaat atattggtga agatggctat
    15361 tattcgatgg tttttgattt tagttatacg gatattgatg tacctgaaac tggtgaatgg
    15421 tttaaagata gccaatggac atggacggat atgcgtacca atatttttac gaatcaatta
    15481 gttacgcaag ataaaggttg gggcgcctta tatttagaaa accacgatca accacgttca
    15541 attaataaat atattccaga agaatggatc aatgattata gtaaaaaaat gttggcgacg
    15601 ctttttatgt tactaagagg tacccctttt atttatcaag gacaagaact aggaatgacc
    15661 aacatagaaa tggcatcatt agaagatttt gatgatgtag ctacgcattc tcagtataaa
    15721 cgggcattag aatatggatt ggcaccagag caagcattga aagctgtcaa taaacgaagt
    15781 cgggataatt ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag attctcaact
    15841 tctgataatg tatggttaaa agtaaatcct aattatccag aactcaatgc agaagctcag
    15901 ttaaccaatc aacagtcggt attcaattat tatcatcaat taattaaatt aagaaaaaat
    15961 agtgtataca aagacgtgct aatctacgga caatttctac cagtgaaaga taaaaatgaa
    16021 catattttgc tttatgaacg ccaattagaa gataaaatcg tattagttat cttaaacgtt
    16081 acagcaacag aacaagaata ttatgtagat gaacagtacc aacaagtatt aatcaataat
    16141 tatgaagatg ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt tgaaagtatt
    16201 gtattagcta attatggagg acaattatga aaattaaaaa cgaagcaatg ctcatcacct
    16261 atgcagatag tttaggaaaa aacatgggcg aattaaaaca agtcttagac aaacatttac
    16321 aaggtgttat tggtggtgta catctgttgc ctttttttcc atcgacaggt gatcgaggct
    16381 ttgcgccgag tgattatacg acagttgatc cagctcttgg aacatgggaa gatgtggaag
    16441 cattaggtga agattattat ttgatgtttg attttatgat taatcacatt tctcgtgaat
    16501 caaaattttt ccaagatttc aaaaaaaacc atgagcagtc accatacaaa gaaatgttta
    16561 tccgtattca tgaatttttc ccagaaaatc gaccaacaca agaagatatt gatttaatct
    16621 acaagcgtaa agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt acgacagaag
    16681 aggtttggaa tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa gaagttgtga
    16741 aagaatttat tcgagaaacc atcaaagata tggcgaacca tggctgttct ttaattcgat
    16801 tagatgcgtt tgcttatgcg attaaaaaat tagataccaa tgatttcttc gtggaaccag
    16861 atatttggga acttttagac gaagtgcgtc aagaagcagc gaaatatgac gtcgaattat
    16921 taccagaaat ccatgagcat tattccattc aaatgaaaat tgccaatcat gattattata
    16981 tctatgactt cgccttgcca atggtcactc tgtattcact ttatagtgga aaatcagaac
    17041 gtttagccaa ttggttgaaa atgagtccga tgaagcagtt tacaacattg gatacgcatg
    17101 atggaattgg tgttgtggac gctcgtgatt tattaacaga tgaagaattg gattacacct
    17161 ctgaggaatt gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt gccaattaca
    17221 ataatttaga tatttatcaa attaacagca cctattatag tgcattaggc gataatgacc
    17281 gtagctatct gttagcacgt gcgattcaag tatttgcgcc agggattcca caaatttatt
    17341 acgttgggtt attagcagga aaaaatgata ttgaactgtt ggaagcaacg aaagaagggc
    17401 gtaatattaa tcgtcattac tatgatttag ccgaaattga agaagaagtg caacggccag
    17461 tgattcaaca attatttgca cttttaaaat ttagaaatga atctgcggcg tttgatttgg
    17521 atggaacgat tgaagtagca acgccatcag aatcagaaat agtgattaca cgtaaaaata
    17581 aaaacggtag tgatagtgca acattaacgg ctaacttgga tacgaaagaa tttatcattg
    17641 agtctaaaga tataaaaatt ttcactagtg ttgcattaaa attttaacct attccataga
    17701 acaaaaaatc ctttatacta gcacttggat gacttatcca agaccaataa aaaggactca
    17761 tacatggata agtataaaac aaatacagca tttaataaat ggttttctgc cataaaatta
    17821 ggaaaacttt ctgtcaactc ccaaaaatgt atttttgatt acaattcgta tcgtaaaaaa
    17881 ctgtcttttg aagctgtgct caagctcttt ctctacgcag ttaatgaaga aaaagaaagt
    17941 ttgcgtgatt tgagtacagc tcttatcgac cgatcgctcc aactagaaac cgatgtgtca
    18001 tcgattagtc atacccaatt gtctcgagca tttaatgcac ttgaacctaa agtgttagaa
    18061 gaaatttttc aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac caagcgaaat
    18121 catctgtatc tgattgattc cagtacgttt tcgcttagtt taaaacgtca taagtgggcg
    18181 actttccgtc aaacaaaatc aggtgtcaag ttacacttaa actattgtta tatggacgat
    18241 actacgatgt atccaactga ttttacctta acaaatgccc atgaacatga tctcaatcaa
    18301 ctaccagtat tggtgaatca accagaggca acttacgtgt ttgatcgtgg gtattaggac
    18361 tttgaaaaga tggatcagat gcattgggat ggctatttct tcgtcactcg gattaaaaaa
    18421 aacaccaaag ttcacgtgat agatactctg gaaacatcgc cagaaacaga aattctacga
    18481 gacgagctgg ttcgtttagg atctaaaacc tatctaaccg ccaatttccg actagtcact
    18541 gttcaagata agaacggacg tgtgtttcaa tttatcacca atcgaatgga cgtctcttca
    18601 aaagaaatct ctgatatgta tcacgctcgt tggcaaatcg aattgttttt caagcacatc
    18661 aaacaacaca tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt gcagaatcaa
    18721 ttgattttaa cgatgatttc cgctctcctc acgtttctca tcaaattgga gacgaaaacg
    18781 gaaaaatcag tgttccaaat caaacgattc tttcgttatt tactgtttca gccctttgaa
    18841 tgttgtctag aaaaattaat tcccacatga gaaaaaagaa atttggctcg ttacttttgg
    18901 ttttcagttg aataacagat aaaaaatttt ctggaaaaag tcatcctctg cgtcagcaca
    18961 gttttttttg acttattttc agaggaaata gttttcagaa tattctaaaa tgttttatgc
    19021 aacactagtg atatttttta gaattagatt tctaatgtgc attttttgag tattaaggag
    19081 caagtataca aagatttata aagctgatta aatttgaata aaaatatgca ggaacgctac
    19141 tatttttagt agtgttcttt tttatatttg aataaaaaat gtggtggtta aatggtggtt
    19201 ataacaaaaa atgaattaat gaaattaggt tatgggaagt atcagtctga gagaatcata
    19261 cgacaggcaa aagcaattat ggtttcaaaa agctatacct attacgaaaa tagaagatta
    19321 ggactagtac ctgttgaatc tgtagaagaa gttttaggta tgaagataga ggtagaggag
    19381 gattttcaga atcatggtta attttaaaaa agataaaaat gggaaatatc attttactgc
    19441 tagtttggga ttcgacaaac gaacagggaa aagagtacaa aaaaacgtag aggggttaaa
    19501 acagttaaag aagaaaaaaa gcgtataccg aacttgtttc taattatgga aaagaagcag
    19561 aaacttctca aagtactatg ttgtttgagg agttttttta taaattattc ctaccttatt
    19621 atcaagggaa agtagaagaa agaacatata aaaatcggtt atctacaatc aaaaaaactt
    19681 ttcttatttc tttaaaatgg agttaagaga aatacgtcca attgacattc aaaagtggca
    19741 aaataattta ttgagcaaat ataaaaattc ttatgtaaga aacatgtatg ggatttttca
    19801 gatgtcatta gatcgagcag ttgttttggg actacttgaa aaaaatcctg caaaagttat
    19861 tggaaatgtg aagaaaagga aaactacagt tgatttttgg acaaaagaag aatttacaaa
    19921 agttatttca actttttata ttgatgattt gtaccaacat tttggtttta tttgtatgtg
    19981 gttacttttt atgaccgtta tgcgtttggg agaagccact gcattaactt ggaaagatat
    20041 tgattttgaa aataaaaact tgcgtgtgaa taaaacactc tattataaga gagccaaaac
    20101 ctatgagttt gtagaacccg aaacggcttc tagtattcgt acaatcgaac tagacgacaa
    20161 tacaattacc tttttaaaag cttggaagga gcgtcaagtt tcaataggag gcatgtcgtt
    20221 tgtactttct tataatggaa cacctactca gaaatataca atccgtcata ttatgagaag
    20281 acatgcaacg atcgttggtg ttcatataat ccgcatacac ggattaaggc atagtcatgc
    20341 ttctttgctt atatcacaat aaaaagatgc acttcgaatt aaagaaaggt taaaacatgc
    20401 tgatgtacaa acgactttag gaacttatgg tcatttgtat ctaaattcat tgagagagat
    20461 aacaaatgat cccacagatt atatccaaac taatttttct aaaatcaaac cgacacctat
    20521 ttacgataat aaaaaataaa tgataaacaa aagtaggatc acaccaaaag aaacacacta
    20581 gaaaaactat aatacaggct tttctagcgt gttgtttctt atttccgcct atttatacat
    20641 tatactcata aagcatttaa tgcatcgatc gtagccgcat tgtctatatg ggtatataaa
    20701 gttgttgtat tggtacttga atgtcccaat tgttgggcag ttaataccaa tgaatttgtc
    20761 tgttggtaga gtctagtagc taatgtatga cgcagtttgt gagggctgac ttgaattcta
    20821 aagccttttg aatattttgc taccatccgt tcgattgcaa taccggatat acgcttggct
    20881 tctccttggt aaattgctag aaataaagct ttttcgtttt ctgttacttt atatcgttct
    20941 ttccgaatag ctagataatt agctaaatat tccattgcga atgctgctat attgacagca
    21001 tctcttttat ttccttttct agttactacc acattattct tatctaaaga taggtcatct
    21061 aaattaatat ttataacctc ggagagtctc aatccagatc ctaaaatcag tgcattgatc
    21121 gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttact caaagtattt
    21181 ttatattcat tggcaatata atctagataa ttttgtgtat ccttatctag aaataatttt
    21241 ttctcaatgg cattggcacg tgcagctaac gtagttttat ctgtgatggt tgctacttga
    21301 tccataggat tattgtctaa gtatggtttt cctagaggat tattggaact tttcgaaaga
    21361 tatttaaata gaacctttaa tgccgtgata tttctattga tagtagaagt agatagacct
    21421 aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt ttcttttgtg
    21481 cgacgtttca aatgagattt gtacaagttt aggtcatagg cagttaactg tcttatcgag
    21541 gtgatagaag tatcaggaac gatagactca ctcagaagcc attcataaaa gagcttataa
    21601 ttttttagat attctaaagt ggttcgaggt gattgatctt tatcctcacg atcttgaaca
    21661 aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg atatatttct
    21721 gtatttttca tgtcattcat cctttcaata agtaggggag aagagtatct ctataattgg
    21781 actataccaa ttatagaatg ttttgtcaat tggtatgctc taaaatttat taatttgttt
    21841 caaaaaaagc atataaacag gtatacgttt aataatttaa aataaatggt atacataaat
    21901 agggaaggaa atagaaggga gcaatctaaa ttttttgaat tttcttaatt aattatatag
    21961 aaagaacaat ctttctatag agttagacat gagagggact atgcttgatt taaaatcaac
    22021 aagcgtatat actgtatata caaagaaaaa ggagggagaa gactatggca attaaagaaa
    22081 gaaaatttag ggaagtgggg aactcagtag tattaactat ttcaaaggag tttcttaaag
    22141 aaaatgggtt aaatcccgga gatacggttt ttttagatga agaaaaactt cgagcggcaa
    22201 ttacaaaaaa acaagcctta tctgatgaaa ttgatttagc aatatctcaa gcattaactg
    22261 aatatgatac tacttttaga gagcttgtcg ataaatgagt attcattacc tagaagccaa
    22321 agatattata aaaatgaatg taatccaaat aaaaaaatac tctcctaatg aaccccttgg
    22381 tgtaaaggat gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct cagcttttgg
    22441 tgaagaggtt tatccttcga tttatgaaaa agcagctatc ctacttatac aattgataaa
    22501 gaaacatcct tttcacaatg caaataaacg tactgctttc ttagctactt ttgtcttttt
    22561 gaaaatcaat ggacagcttt tagctattga tcaaaaagat gctgtaaatt tagcggtata
    22621 tattgctacg tatgataagg atttcgatca actaaaatat gaagtgatca acactataaa
    22681 gacacatacg cagcccattt aaggagttac tgtatgtgtc tttatagggt tattagctgc
    22741 tatagcgaaa taattgaagt aaattacaaa tagctataat tcaaatccaa ctatatagaa
    22801 aacgtaaatt ttctatatag ttggattaag ataacgacta tttattttat catattttta
    22861 ctatacccta tacttttact tggtatattt atgaataatc gcaaatttac ccctatacta
    22921 ctagtttgta agttagatta ataaaaatac taaagttatt tgttctttta ttaaatacac
    22981 ctacaactta taaattgatg gtatttagat aacacgaaag aataaaaata acagctgata
    23041 gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt tagtgcgtat gaacttgctt
    23101 ctgaaaactt atgaaatgaa agacctttta catcttgcag aaatcgttac tgggattaaa
    23161 ctaagcaata aaaactaatg ttcccctcat tctttgaact tctactattt gatttattta
    23221 ttctgataaa tgacgatttc accttcagga tcttcaaatc ccgtatagaa tttatttaag
    23281 atctcttcag tatcagtaat aggataggcg ataggccaca taccctttga tggttagaat
    23341 aaactgctca aaatctgtat cgtatgttgg ctttagcata ttaacaaagg ctatctccaa
    23401 attataaacc ataagcgttg aatcgttctt aacaatttca attcttccta acccaaagtc
    23461 agaagatata attaccggac ttgccttacc cttcatgtgg gttgttgaga ttccatattt
    23521 aaagggaatt gtcggtacta tcggcggtct gaaaaacatc ccaaaaataa aaaaatcttt
    23581 cccctgctag tctcctaagt ccctttgtag cggatatggc accataatct cattttatcg
    23641 aagtgagtca tggtttatct gttggccaac caaaattgat acaatctaac gatgcaccct
    23701 ctacggcatt ctaggggagt gcacttgcat tcacccaata aaaaccggca gttagcaaag
    23761 aacttatgta gtcgtatgtt cttactgtta acatacaaga ggtctccttt ccacaattgc
    23821 ttagcagtaa acgggaattt acaaagctaa tctcttccat tttcttatct ttgttctaaa
    23881 agtcccaaat ggagcaactg tatttacatg aataaattta tatacttccc atacagccgt
    23941 tttggttgcc tcgtctgccc attttctttt gtgtgcagtg aatagatctt catcactcat
    24001 tgaatcaatc atcatgttaa tatcatcaat attacgattg agaatttcct ctaattcttt
    24061 caatggtaga tgtgcatagg tatttgtaaa ccacttatat aactctccta actgattcca
    24121 tttaaattga tcagaaggcg ttttaacaga taatccattt ctttcatcat tttcccattt
    24181 tagaattaaa ttcgtccaac caacttgata tgctaaattt tcagcaggtg ttctatccac
    24241 ttcctcaact cttttatcct tcaattcttc aggaatagtt tcaaattcta aaatatattt
    24301 agtataagtc ctatttattt ctttttttaa ttcttcttta tttttatatg ttctcattat
    24361 attacctcca taaattccta attgtctcct ttaattcatc tataatttca tccacgatat
    24421 tttaatccgt tctacaatgc atatctgata tgcattgact gtctaactca tatgtgaatg
    24481 atttataaaa tatcccaacg tctcatctaa agaaatgttg atcgtcagca aggagctaaa
    24541 aaatacgaat atatttcttt tgtagtaaaa tcggactggt ttgcatatca ttttcttcgt
    24601 aaatatcaaa atcatgttaa ctactatcaa ttactaataa aaactaccac tttaagcgtt
    24661 aagaactgtt aagtttagtt aagtgaggat agttcacaaa atccattgtt aaacttcacg
    24721 aaagtggtaa aactcaaaat gctttatata ggattacagc atcgctcttt cttcgattgc
    24781 gcgttgggta aaaaatatta tgaagtaaaa attgacgatg acacgttcct tacggctcat
    24841 cagatgaaac agctacaaaa acgaaatgat caactcgaag aaaatttaat tctaaaaaag
    24901 cgattgccat attcacgcct cgctcgacga actattaaag gtcaagattt acctgaagat
    24961 catccttttt aggaaattga agctttttat gttgcacaat gcatttataa tactacaatg
    25021 atgttttcac cagatattct tctagggtgc ggtgtaatga aacagataca cttgcaaaag
    25081 aaaattcacc gcgaatttac taaaattatg aataattatg ttaatcttcc gaaatttttt
    25141 tccaaaatta agggatcaag ctggtgttat agattgttta aggttggcaa aaaaataact
    25201 tctttttaaa caattataga aaaccgatac tctgttaagt tggttgacac ggtgtcgatt
    25261 tttatgactt tcttattctg aaacttcact aacggacagt acctaaaaag tctatagtat
    25321 ggacgtttac gagtactatt ttatcaaaaa tgctgctcat ttcaacttat atgttttagt
    25381 gaactacccc cacttagctt ggataggcta caattaacta gacagaaaaa ttaaggtgtg
    25441 tagactagaa gaaaacatac caggaggaat ttttatgtct aagagaacac gaagaacttt
    25501 ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta gctggaaagc cacgtgtaga
    25561 aatcattcga gaatatgaac taacggcttc agcatttgac aaatgggtaa agcaatctaa
    25621 aacgagt
//
LOCUS       DAFCHQ010000037        25595 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000037 DAFCHQ010000000
VERSION     DAFCHQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.037"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            128..2188
                     /locus_tag="LNC71_001551"
     CDS             128..2188
                     /locus_tag="LNC71_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBK6466624.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2271..2840
                     /locus_tag="LNC71_001552"
     CDS             2271..2840
                     /locus_tag="LNC71_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466625.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3015..3821)
                     /locus_tag="LNC71_001553"
     CDS             complement(3015..3821)
                     /locus_tag="LNC71_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466626.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3959..5473
                     /locus_tag="LNC71_001554"
     CDS             3959..5473
                     /locus_tag="LNC71_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBK6466627.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5876..6859)
                     /locus_tag="LNC71_001555"
     CDS             complement(5876..6859)
                     /locus_tag="LNC71_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBK6466628.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6864..7511)
                     /locus_tag="LNC71_001556"
     CDS             complement(6864..7511)
                     /locus_tag="LNC71_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6466629.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7596..8228)
                     /locus_tag="LNC71_001557"
     CDS             complement(7596..8228)
                     /locus_tag="LNC71_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6466630.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8244..8795)
                     /gene="coaC"
                     /locus_tag="LNC71_001558"
     CDS             complement(8244..8795)
                     /gene="coaC"
                     /locus_tag="LNC71_001558"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBK6466631.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8792..9529)
                     /locus_tag="LNC71_001559"
     CDS             complement(8792..9529)
                     /locus_tag="LNC71_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBK6466632.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9526..10515)
                     /locus_tag="LNC71_001560"
     CDS             complement(9526..10515)
                     /locus_tag="LNC71_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBK6466633.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10773..11315
                     /locus_tag="LNC71_001561"
     CDS             10773..11315
                     /locus_tag="LNC71_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6466634.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11332..12417
                     /locus_tag="LNC71_001562"
     CDS             11332..12417
                     /locus_tag="LNC71_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466635.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12429..13235
                     /locus_tag="LNC71_001563"
     CDS             12429..13235
                     /locus_tag="LNC71_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466636.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13236..14063
                     /locus_tag="LNC71_001564"
     CDS             13236..14063
                     /locus_tag="LNC71_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466637.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14060..15133
                     /locus_tag="LNC71_001565"
     CDS             14060..15133
                     /locus_tag="LNC71_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6466638.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15561..18257
                     /locus_tag="LNC71_001566"
     CDS             15561..18257
                     /locus_tag="LNC71_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6466639.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18334..18822
                     /locus_tag="LNC71_001567"
     CDS             18334..18822
                     /locus_tag="LNC71_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6466640.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18834..19328
                     /locus_tag="LNC71_001568"
     CDS             18834..19328
                     /locus_tag="LNC71_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6466641.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19349..20158
                     /locus_tag="LNC71_001569"
     CDS             19349..20158
                     /locus_tag="LNC71_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6466642.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20155..20976
                     /locus_tag="LNC71_001570"
     CDS             20155..20976
                     /locus_tag="LNC71_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6466643.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21027..23126
                     /locus_tag="LNC71_001571"
     CDS             21027..23126
                     /locus_tag="LNC71_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBK6466644.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23123..24241
                     /locus_tag="LNC71_001572"
     CDS             23123..24241
                     /locus_tag="LNC71_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBK6466645.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24280..25158
                     /locus_tag="LNC71_001573"
     CDS             24280..25158
                     /locus_tag="LNC71_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6466646.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25349..25501
                     /locus_tag="LNC71_001574"
     CDS             25349..25501
                     /locus_tag="LNC71_001574"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBK6466647.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8097 a         4451 c         5418 g         7629 t
ORIGIN      
        1 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
       61 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
      121 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
      181 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
      241 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
      301 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
      361 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
      421 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
      481 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
      541 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
      601 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
      661 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
      721 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
      781 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
      841 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
      901 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
      961 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     1021 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     1081 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     1141 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     1201 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     1261 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     1321 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     1381 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     1441 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     1501 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     1561 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     1621 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     1681 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     1741 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     1801 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     1861 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     1921 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     1981 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     2041 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     2101 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     2161 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     2221 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     2281 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     2341 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     2401 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     2461 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     2521 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     2581 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     2641 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     2701 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     2761 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     2821 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     2881 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     2941 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     3001 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     3061 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     3121 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     3181 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     3241 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     3301 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     3361 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     3421 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     3481 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     3541 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     3601 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     3661 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     3721 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     3781 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     3841 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     3901 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     3961 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     4021 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     4081 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     4141 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     4201 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     4261 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     4321 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     4381 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     4441 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     4501 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     4561 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     4621 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     4681 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     4741 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     4801 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     4861 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     4921 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     4981 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     5041 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     5101 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
     5161 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
     5221 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
     5281 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
     5341 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
     5401 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
     5461 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
     5521 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa agctgccctg
     5581 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggttggac
     5641 ttattaccgc agatgcaagc ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa
     5701 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagaaat
     5761 tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc
     5821 ttatttcttg gagttaaacg cttctgtccc agcctctttt ttgatcagca tgattttatt
     5881 cagttatctt caaaatgatt tttccgatgt ttttgtttgc ttccatatat tcatgcgctt
     5941 gtttgactgc gctgaattgg aatgtacggt caatgattgg ccgaatcttg ccattttcta
     6001 gaaaaggccc agctttttgc ataaaagttt gtgcgagctc tgctttatac tgatcacttc
     6061 taggtgtcaa taatgtacca agcagttgga cacgtttccc aatcaataca ccaaggtcta
     6121 tctcaggcac gatggtccca cctagcatac caatcaagac ccaacgtcca tcaacggcta
     6181 tagaagctaa attcttttcc cagtaagaag cgccgatgaa atccaaaata acatcgactc
     6241 cccgaccttc agtgattttt cgtacctctt cttgaaagtc ttgtttttta tagttgatca
     6301 attcatctgc accaagttct ttacaaaagg cgagtttttc ttctgatcct gcagtaacga
     6361 tgattttcgc gtgagaaagc tgtctagcta attggatcgc tgctgttccc acaccacttg
     6421 ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt tccaagccag taaagtgttt
     6481 gataagcagt caaaaaaact tctgagattc cagcagcaga aacatagtct agcgtatcag
     6541 ggagaaggat tgcacgatcg gcaggcatca cagcatattc ggcataacca cctagattga
     6601 ctaatccgta tacttttgtc ccaggagtaa aagtggagat atcactttta ttctcaacta
     6661 cctctccagc tacttcgact cccaaaatag ggtagggagg tttcaaagag actgattctc
     6721 ttttcataat atctgttcga ttgacagctg ctgtatggac tttgatcagt aattcgcctt
     6781 gttttggttt aggaacaggc acttcaattt gttctaatac atctacagaa ccaggatgct
     6841 tgattgaaat ggcacgcatg gatctattcc tcctttgccc attcttggaa tgctgtattc
     6901 aactctttgc taccttcaga aagtactaga gtcgtacgag aaatatccgt ttcttccact
     6961 agagcggcaa gtacagcagc aactgtagag cgagtgatcg tttttgatgc cttctcatta
     7021 ggcttagtta attgaatgga agtaacttct tcatcgttag tcaatgtgac tggttgaaga
     7081 atgacaaaat ctaagttagt tcgtttcagc cattcatctg cataatgttt agtaatataa
     7141 taatctgtca tggactcagg ccattttgct ggttcatctg cataaacagc actgaccatc
     7201 agatagcgag agacatgcgc attttcagct gcgatcactg ttttgattgc gccatccaag
     7261 tctacttgca gaagattctt tccttgagag ccggcagcaa aaatcaatac atcgatgcct
     7321 ttgaatgctt cggtcatttt ttccacggac caagttaaat caaaagagac atacgaaaca
     7381 ttctcttttt ttactaatgt ttgggaagca acatctctca cccctgcaaa aatttcatgg
     7441 gaacttggag ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc acctacaaca
     7501 aaaattttca tttggatcat cctttcgacg tcaaatcttt ccattcaata gtaacaccaa
     7561 aagagaaaaa aatcagagat cggctacgat gactatcagt tagtaagaga agggtgcttg
     7621 aagtatctta acaagacatt gcatactgct gcagtcgcaa gacctgcgac gattccttca
     7681 ggtataccac ttgtaaaaat aaccgctaga atagctgaat aaacagcatt taaatttgtt
     7741 ccgatgactt tggcataatc ttgctggaat aaaaagtaga tcatattcat cactaaaatc
     7801 gtattggtca atgaaccgga aagtccagct aagaataaag aaacggaatc tgctttttgt
     7861 ttcattagtt tgcgcagacc tttataaacg aaataaggaa ccaccccaat caagatgcga
     7921 ggaataaacg cgatcagcaa ggctttccag cttccatgtt ctgttccgat gactgggata
     7981 aacggagaaa agacaaaaga caaaggtgtt tggataactg tgctacggat catgctgatc
     8041 agtccaaatg taccgccaag aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg
     8101 attacaggaa tatgcatggt tgttgcattg attggaccga tagggataaa acctaaaggc
     8161 gtaacagcta ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa gttttttgta
     8221 tttttcataa gtactccttt tttctaagca tttctttcat taagagtcag catgacctgt
     8281 tcgatgatcg tctgaatatc agctaaggca ccttttccaa aatctccaca agctaaaaga
     8341 gattctcttg gttcaatttc ctgatagccg acagatctca atgtttccag attacgctga
     8401 gtgatcgggt tttgatacat attcgtgttc atggcagggg caatcaattt tggtgttccc
     8461 actggaagcg ccattgcgac tgttgaaaga agatcgtctg caatgccgtt tgctagcttg
     8521 ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt tgctagttcg
     8581 atatgattga ttacctgcgg atttgattct tgcatgacgt ctgtatggac tgcgcgcttc
     8641 gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga cataatcaca
     8701 tctacctggg cacctaattt gacgaattga cttgtaagat cagcgctttt gtaagcagaa
     8761 atacttccag taacgcctag taagatcttt ttcattttga ttcctccagt ctaattttct
     8821 cagtaatcaa tgcagcaatc tcagatttac tttgagcttc ttccactgtt ccatctttag
     8881 ataagagata gccgtggtgt gtctcgccat ggatttccgt caaatcattt gcgaaaataa
     8941 aatcagtttt gttatttatc agactatttt ttgcgactgc cagaagagat tcctttggta
     9001 catctactaa tagtttgaaa ccaatgattt ttgcttttgg ctgccattca cgtagataag
     9061 aaatgatttt tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta gaagagatct
     9121 ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca attgtccagt tcttcttgcg
     9181 ttgaagaagt gggtaggttt tttgccagct gttcttctga aaaactaaaa gcaggagtaa
     9241 aatcactgac tgccatactg tggataatcg catcgtagtg ttcttttttc gttagttctt
     9301 ctaattgtaa aaataagtcc agtgcagatt cgatcgtata aatcgttata ttggagtgtc
     9361 ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag
     9421 ctaaataatc agctaatgct tgccctaatc gtcctgtcga atgatttgta atagcacgta
     9481 cttgatcgat tttttcagtc gttcctccag ctgtgattag aatcttcatt tatggtcacc
     9541 tacatttact agtgttcttc cttggtgtgt tccagcaagt agtttagatg ctgtttcagg
     9601 caattgatcc agagtaatct cttggacgac cagttcatct gcaatctgca aatccgccat
     9661 ctgttgccat aaagaaagac gttgatctat tggtacattg acagaatcaa ttccaatcaa
     9721 ctggatgttt cgcagaataa aaggcaagac agtcgcgttt agtttgattc ccccagcatt
     9781 gccacataaa gctactgcac cgttataaga gatcaatgga agaagactgc ttagctgctc
     9841 gccacctact gtgtcgatga catagtcgat ttgctgtttt cctaaagctt ttgttgtctc
     9901 tggcagaaat tcatcaggtc gtacgacttg caaagcacct agacttttta gccatgtgac
     9961 agactcttct tttctggaaa aggtaatgat tgactggaat cctagtttat gtaacagggc
    10021 tatcgctgta ctgcctacac cgccagaagc accagtaacg acaacttttt tatcggaggt
    10081 cataccttga cggattaaag cgttcacaca caacatagca gtaaaccctg ctgtaccaag
    10141 gatcatcgct tgacgcagcg tcatattttt tggtagagga actaaccagt ctcctggtac
    10201 tttttgatat tggctataac cgcctggatg ggtgacgcca agcccaaaac cagtagcaag
    10261 tacaagatct tcttttgaaa aacgtggatt gttactttgg acgacagttc cggataaatc
    10321 aatccctgga gtcatcggat atgttcgtat cacctgacca gactcgctca ttgccaatgc
    10381 atctttataa ttgacatcag aataggcaat ttttaccaag acttcatttt cagccaactc
    10441 agtaaatggc agatcactga ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga
    10501 tcgaaaagcg ttcattcgtg agacactcct tttaaaattt tcttttatca taccataaag
    10561 cctactcttt tattcttgtt cttccgtaaa tttagacaaa aattaggtga aaaataaaga
    10621 atcaaacaac tagaatttat tccaaaagat aaagaaatat tcagcaaaaa aaattttaca
    10681 aagggttgcc tttttcaata aatttcgtat actaatacga gtggtagtaa acaggaagtt
    10741 tactattaga aaacttcgat tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt
    10801 tgcgcatcca aaagaatttg acacaagaag aactaggcga acgcacagat ttgtcaaaag
    10861 ggtatatttc ccagttggaa cgagatttaa gttcaccatc aatggaaaca ttcttttcta
    10921 ttcttgaagt cttaggtgtg actccagaag aattttttca tcaagaagca gcgaatctgc
    10981 aagtggttta ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac gggtatgaat
    11041 tggaatggct cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg
    11101 aaggtgcagg agaatataag acgtttgaac cttcattatc tgaaacgttt atttttgtat
    11161 tagaagggga attagagtta agcttaggag agacggtcta tgtggcaaaa gcaggacagt
    11221 cgatctatta tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa
    11281 aagtattgat tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa
    11341 ccaatcattt catttgatca agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa
    11401 gtaagttttg agatcgaaca aggaaaattt tatacactgc ttggtccttc cggctgtggt
    11461 aaaacaacga tcctacgtat tatagctgga ttcaccgaag ctacagaagg ggacatctat
    11521 tttgaaggga aacggttgaa tgatttgcca gctaataagc gacaagtaaa tacagtcttc
    11581 caagattatg cactttttcc tcatatgaat gtttttgata acgttgcttt tggattgaag
    11641 atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc
    11701 cagcttcctg gatacgaaaa acgggagatt agtgaaatgt ctggtggtca aagacagcgt
    11761 gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta
    11821 tcggcgcttg atttgaaact acgtacagat atgcagtatg aactgcggga tctgcaacaa
    11881 cgattaggaa tcacgtttat ttttgttact catgaccaag aagaagcgtt ggcaatgagt
    11941 gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc cgtagatatt
    12001 tatgatgagc caatcaatca tttcgttgct gattttgttg gggagagcaa tatcgtggac
    12061 ggtgtaatga tcgaagataa tctagttgag tttgttggta agcaatttga atgtgtcgat
    12121 ggcgggatgc gtctaaatga accagttgaa gttgttttac gcccagagga tttgacgatc
    12181 acgacaccag ataaaggaaa actagtcgta acggtcgata ctcagctgtt tcgtggtgtc
    12241 cattatgaaa tcatctgtta tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa
    12301 aaagctaaag aaggaagcaa agtaggattg gcctttgagc cagaagatat ccatgtcatg
    12361 cgtttcaacg agtctgaaga agaattcgat gcacgtcttg acagctacga agaatagggg
    12421 ggacaagaat gaaaacaatg cgcaggatct attccgtccc ttatattttg tggctcggtt
    12481 tattcgtgat cgcgcctgta ttaatgatca tttatcaatc tttttttgat atgaatgggc
    12541 aattcacgct gaataattat ctatcttatt ttacgtcggg gacatcgctt tctatgacga
    12601 tcaattctgt atggtatgca tttttgatca cgtttttcac gctgatcatc agttacccga
    12661 ctgcttattt tttaacaaaa ctgaagcaca agcaattatg gctgatgctg attattttgc
    12721 ctacatgggt gaatctgctg cttaaagctt acgcatttat tgggattttc agtgtgcacg
    12781 gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc gaaacagata ttattcacag
    12841 acttcagttt tctgttcgtg gcaacgtata tcgagattcc atttatgatt atgccaattt
    12901 tcaatgcatt ggaagagatg aatccttctt tgatcagtgc tagtagagat ttaggcgcga
    12961 atagctttga gaccttccga cgagtgattt ttccgttatc attaaatggt gtgaaaagtg
    13021 gtgtccaagc agtatttatt ccttctcttt ctttattcat gctgactcgt ctgattggcg
    13081 gaaatcgggt gatcacttta ggaacggcta tcgaacagca tttcttggtt acacaaaact
    13141 gggggatggg ttctacaatc ggtgtgattt taatcattgc tatgttcatc gtgatgctcc
    13201 tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc
    13261 tttatttagg gatcgtcttt ttattcttat atgcaccgat cttctatctg attttttatt
    13321 ccttcaatga aggaggaacg atgaacagct ttacaggatt tacttgggaa aattatgaag
    13381 ctgtttttga agatacacgt ttgatcatta tcgtattgaa tacgttcatg ctggctttct
    13441 tatctgcatt gatcgccacg atcatcggga catttggggc tatgggaatc tattacacga
    13501 aaaaacgacg tgcacggaca gcgttgttga gcatgaataa catcttatta gtctctcctg
    13561 atgttattat cggtgctagt ttcttgattt tctttacact actaggattc ggattaggtt
    13621 ttcagagtgt attgctttcc catattgctt tcagcattcc aatcgtcgtc ttgatggtct
    13681 tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc ggctagagac cttggtgcta
    13741 ataatttcca agtagtcaaa aatatcattt tgccttatct ttctccagga atcatagctg
    13801 gatattttat ggcatttacc tattctcttg atgattttgc ggttacattt ttcgtcacag
    13861 gaaatggctt tacgacgctc tcagtagaga tctattctcg tgcaagacaa ggaatcagtt
    13921 tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc catgatattg gtcattggat
    13981 actactttat tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg
    14041 agggggtggc acggctgcga tgaaaaaatt acaatctctg tttttaggta ttctcatcat
    14101 tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca tgagtggagc
    14161 aaaggtactt accatctata actggggaga ttatatcgat cctagtttga tcacgaaatt
    14221 tgaaaaagaa tatggataca aagtgaatta tgaaacattt gattctaatg aagcgatgtt
    14281 cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct ataccaagtg aatatatgat
    14341 ccaaaaaatg atcaaagaaa aactagtact tccattagat cattcgaaga tcaaaggatt
    14401 aaataatatt gatccacgat ttttagactt ggattttgat ccagataatc agtattcgat
    14461 tccatatttt tggggaactt tagggatcgt atataacgat aaattctttg atgcaaatca
    14521 aattaagcat tgggatgatt tgtggaagcc ggaattaaaa gacagtttga tgctgattga
    14581 cggtgcacga gaagttatgg ggctttcttt gaatagttta ggctattctt tgaatagtaa
    14641 gaatatggca cagctgacag aagcagcaaa taaattgaat catttaacac caaatgtcaa
    14701 agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt
    14761 gacattttct ggcgaagctg ccgatatgat gtgggaaaat gaacatttac attatgtgat
    14821 cccaccagaa ggatccaatc tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa
    14881 taaagaaggt gcctatgatt tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa
    14941 tgcagagtat attggttatt ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga
    15001 catttcgtcg gacgaacaat tctatccatc tgatgacacg atctcccatc ttgaagtcta
    15061 caaagatcta ggatcgaaat acctaggcat ctataatgac ctattcttag aattcaagat
    15121 gtataggaga taatggcaaa actcagtaag aatggggact tatcaacgtt ctaaagagct
    15181 aaaaatcgtc ctttttctat ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc
    15241 gctttttttg tgttatttta ttttttctac cacgtttttc taccagagaa aagtggacaa
    15301 aaccattgta aaatagggcc cgagacgggc tagtaaatct aagattgaaa aagttacaga
    15361 aggataaagt aattggaaac tatgcaaata tacttaaccc ttctgtaatc tattaataaa
    15421 actaatatat gaatccagtt caatatttgt agctatgaaa aaacataaaa atcatattag
    15481 ataaaaacta aattggtaga taaattattt tgtcagatat ctgaggcaaa tattcgacta
    15541 tttgtgtttc aaagtaaaaa ttggaataca aatatagaat agacatattg cagccgatag
    15601 tttattatgc ttttagacac attaaactgg aggaaaaaat gaaaaagagc gctaaagatc
    15661 gagtttttga tgagctgata cgactaacag ggaatatgtc ttctgtcact gccgagaaaa
    15721 tttcgaagaa tctaggtatc tctcgtcaga atgccagcca ttatttgacg agattagtgg
    15781 aagataaaaa ggtcgagaaa ttaccgggaa agcctgtttt gtggaagcct ttagatgaat
    15841 atgcggttgt agataatacg gagcaaataa acgaagcctt tcattctgta gttggtcatg
    15901 acggaagtct gagagaggtc attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg
    15961 gactcagtgt attaataaat ggagccacgg gtgttggaaa aagtttttta gccacaaaaa
    16021 tttttgaata cgctatccat gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa
    16081 actgtgccga ttatgcggac aatcccgaac tgctttcggc aacactgttt ggatacaaga
    16141 agggggcctt tacgggggct gaaaaggata ccgatggttt attggcaacg gccaacaacg
    16201 gctatctttt tctcgatgag gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc
    16261 tatttattga tactggaaac tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta
    16321 aggttcgttt tattttcgcc acgacggaaa aaggtgaaaa ttatttatta gacacctttg
    16381 atcggcgtat tcaaattagt gttatgttgc caacttttga agatcgcccc attcgagaac
    16441 gattagagct gattcaatta ttttttcaaa atgaagcgga tgttttacaa aaagatatta
    16501 ttgtttcaag agaagccttg tctttaatgt tatctcatcc ctttagtggg aatattggta
    16561 agctgaagaa tatcattaaa atcagttgtg cggatgtgta tagtcgaaca caagaagaac
    16621 cattaacgat tgggaaaaat gagatcttga ttcagttaaa tatgctggaa tccgaagtag
    16681 aaagcttgcc gattgctagt ttattgataa agaaaaatca aaagataaaa acggatcgaa
    16741 tcaaaccgtt tgattatgat tcgattttgg aaaaaattac tcagctgata aatgaaggta
    16801 atttcaatga aaatttttct tcgataaaga atgaagtaca gcatttggtg catcacattg
    16861 ataaggattc ttttttatca atgagtagaa aattgatgat tcagatgttt agaaaggtct
    16921 gggtatccgt tttaggaaaa acgtacggat tgacgacgag tggacctgtt gcagaaatta
    16981 ttagcagatc ctatgtcatt tccccaagct ataagacaga cttaaccggg ataaatcgta
    17041 cattagctgc aaatctacct cggacgactt acttatgtaa tcagttcttg gagcatttac
    17101 caccgctctc agaaaaggac gagaactttc taagactgct tattgtcgtg actttatctg
    17161 attatgtaga tgaatccatc gagctgaagg gcctgttgct tgctcacgga gatagtacgg
    17221 ctagcagcat tcaagcagta gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg
    17281 atatgccagt agagagtaat gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac
    17341 ggcaagtgaa tacagatagt ctagtgctga tcgtagatat gggctcttta aatcaaattt
    17401 acacacagat caagaacgat ttaaaagggg agttacttct tctgaataat ttgacgactt
    17461 ccattgcact tgatattggc ttgaagatga cgatgaatgc gccatttaaa cagattgcgg
    17521 aggatgcagc agacaagtat tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta
    17581 gcaacattat catttcctgt atgtcgggtt tggggatttc ggacaaattg aaagatgttt
    17641 ttcgtcatca tttatcggat gaatccatcg aagttttcac gaaggactac agagaactac
    17701 gtgaattaat cgaccaaaat gatgaaactt attttgacaa gacaaaattg gtcatcacga
    17761 cttcggatct tccacgttcc ttttcaattc ctaatattaa tgtttatgac atcttagacg
    17821 gtgatggagc agccaatttg gagcatattt tgagtcctga aatgaagccg gcagaattta
    17881 accgcttgat tcaggagcta gtaaaattct tttcgttgga aggaattgcg gaccgtttga
    17941 actttctgaa cccaacgatc gtgatgagtg aagtggaaac agtcatttct aaatatgaaa
    18001 attactatca tttcacttta aatggccgtg taaaactaaa tctctatatg catacggcat
    18061 tgatgatgga acgtttattc ttggctcgga cagaaaaatc tgaagaaccg aattatgatt
    18121 tgaaaccaga tgaagatgaa ttttattatg tttctagaag tatttttcaa tcaatggaga
    18181 tgaaatataa ttttaaagtc aatgcttatg agctttcgct gctctatgaa ttattaggac
    18241 cgtatattca aaaataaagt gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca
    18301 ctttttttaa tgtgaattaa cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt
    18361 gcttatataa aagtgaacag aaagaaagga attaaccata tgaagaaaat tctaatagcg
    18421 actcacggtc acttagcttc aggctttctt agcagtattc aattactgac ggggaaggtt
    18481 caagagatta cggtgattaa tgcttatgtg gatgagtgtg attttgaaca ggagttgaat
    18541 gtatttgtaa accaggtgaa cgagcaggat cagatttttg tttttaccga tttatttggc
    18601 ggaagtgtca atcagaagat taccaaaacc tttttggaga aagagattgc tgtaactatt
    18661 ttgtctggct ttaatctgcc aattctgctc gaaatagtat tggcaaccaa ggaattaaca
    18721 aaggaggata ttcaggaact ggtaaataaa tgccaacagg aattaaagat gaccattctt
    18781 gatgaggtac atgtagtaaa tgaagaggaa gatttttttt aggaggaaga aaaatgatta
    18841 aagcagtgcg aattgatgaa cgattaattc atggacaagt agcgatgact tggacaaaga
    18901 gtctaaattt aaccgggtta gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa
    18961 agatgacatt gaagatggct gcaccatcga atgtcaaagt cattattaaa acagttgatg
    19021 acgcgattca tttgttaaaa gatccgcggg cgaaggacat gcgcttgatg gttttagtcc
    19081 ctaatgtcaa agatgcggta aaagttgcga aagctttccc aacggaaatc gaattaatga
    19141 acgtggggaa tgctgggaaa atgacaccgc catcggaaga taagaaaacc ttatcaaagg
    19201 aagtgatgtt aacgacgtca gaaattcaag acttaaaaga attagttgaa ctttatccag
    19261 ataccgtttt tcagcctaca ccggcgatgg aaaaacgttc agctatctct attttgagtg
    19321 atttttagaa aaaggaagga gaaaaattat gtatgatgca tttgtagtcg ctttggccgt
    19381 ttttattgga gttgctggac atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt
    19441 tgttgctccg ttagcaggag cgttgatggg ggatttgcag acggggttga ttgtcggggc
    19501 gtcactagaa gccatcttta tgggtgtcgt caatatcgga atctctagta cggcggagcc
    19561 ttctttggct gctgggctgg ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc
    19621 gattattccc attgtctttc cactagcagt attaggatta cagttaatga atatgatttt
    19681 ttcttttgta tgtggaccga tggctgctaa atttttcaaa tttgcaaaat ctggtgatca
    19741 aaaaggaatc atcaatctgc acttctttat gtggtttgtc cactatgcgc tttatgctct
    19801 tattccattc tttgccgttt tatttggagc agatgttgtc aaaagcgtct tggataatat
    19861 tccagaggtc atcatgaacg gattaacagt cgctggaaat ctgctgccgg cagttgggat
    19921 ggcgatgctg ttacggatgc tttggagtaa aaatattgct gtgtggtttt tctttggggc
    19981 cgttgttatg gcgtatctcg agttaccatt gattgcaatt gccgcagttg gaacgattat
    20041 tgcaattgtt atagcccaaa gagatctaca actaaaaaat atatctgttc atgcagttca
    20101 aagcgatgct gctgtaaatg caacgcttga ccaagaagag gaggatttct ttcaatgaaa
    20161 ttagctgatg gaatttctaa agatgagaaa aaattggttc ggaagatgtt ttggcgctcg
    20221 atgactatgt atgcttcggt gaatccggtg acgatgggcg gcggtggttt cgcctattct
    20281 atgatgccct tcattaatca tttctacaaa agtgaagaag atcgagctgc ggcgctggaa
    20341 cgtcatacac agtatttttc gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg
    20401 gcatcgatgg agaaggaaaa ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg
    20461 atcaaaacga gtttgatggg accattggct ggaattggcg attcattgtt ctggggagtt
    20521 tggcgtgtcg ttaccgcggg actagcgatc aacttagcca atgccggaaa tattttggca
    20581 ccaattatct ttttagttat gttcaatata cctaattact tgatgcgata ctatggcggc
    20641 gttttaggct attcattagg ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt
    20701 gaaattctga caaaagctgc tagtatcttg ggtctgatca tggtaggtgc aatgacgatc
    20761 agtatggtca aattcaatac cgttttgagc ttttcaatga agggcgaaga actaatgaat
    20821 gtccaagaaa tcctcgatag tatttttaaa ggaattgtac cgttattggt aacattgagc
    20881 tgtttcaaat tactagataa aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc
    20941 ttgggaattt tattgtcatt acttggaatc gcatagtagg aagctgcagc tactaggctg
    21001 cagttttttt agcaaggagg gaaaagatga ctattaatga agtaatttat tcacccaaac
    21061 acccattttt taatgctgag ttaaacgacg gaaatgtgac tacttcaaac gaatacagct
    21121 actatcggaa tattcctgat gtaaagggat tgaccttcga tgtaagtaat caaaagacat
    21181 tagcaaacct tgatgtttgc gggacgattc aaacgttgac cttttatcga gaaaatttac
    21241 taactgagga aaaaccaggt gtttgggtca ataaacagct ctctcaaacc aattcgttat
    21301 ccttaaaggt tgaagtaaac gggattgtct accatttgtc agaatcggat catcgtattg
    21361 aagtcgatgt gattcaagac tgtttgccta gatatattca tcattattcg acgtttaagg
    21421 tcattgtagt atcctttgct cccatactgg ctaataagcg tttaagtatg cttgttcaac
    21481 aggtttacat tgtgaatgaa acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt
    21541 cgtatcaaga gaagtattcg gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg
    21601 acacgatcgc acaacaggaa gtggcaagct tcgccattgc ttttgtggac ccaaatgctt
    21661 ttgaagaagt aatgactttt caggaatctg atatcgaaaa atggctgaag gaaacattga
    21721 actattttgc tcagctattt gggcagctgt caatgccaaa cgatagagtt gtccatctct
    21781 atcgtcgtgc actttatcaa agcttttcta gtttcggtat gaatcgacag ggacaggtcg
    21841 tcggcagcaa ttggggcagc tatcctgcta ctaatcgaat ttggaataag gatatgtatt
    21901 acgcatcatt gccattcacc atgtttgaac cggagttatg tcaaaaaacg gttctatggt
    21961 ttgatcaata tggcgttaag tttcctggtt caaaatttac aggaggtatc aatcattctc
    22021 tgagtaattc gttatccagt gcgttactgg ctagtcttta ttttgaacat actggggata
    22081 caaaattctt tgagcggtgt ccgcaggttc tactgaatgc ttcaagaatt attgaggaca
    22141 tattagctca acgtcggtca ggggaaccaa tgctttttcc atctgtttgg ttatcagacg
    22201 cctttgcact tggaaagtat cataccggca gcaatctttg tctttggaag gcctgtattg
    22261 gacttgcaga aatttttgaa gccctagaac aactgagtct tagcaagcga tacaaaaaca
    22321 ttgcctgtaa actaaaggaa tcaatttgta aacaaatgac tatcgatggt ccttttggtg
    22381 agcaattttt ggagggaatc ggtgatgagg agaaaacaac ctattcagtg aagcactatc
    22441 aaaaaccgat tcttgagcaa gggttaatct ttttaagtga tgtgattgtg gatggaaaaa
    22501 ttaatttatt gatgcatgat ggagaagaat cagacactac gttgatgcct ttttatcaat
    22561 ttttagataa taaagatcat ttatatcaga acaccatggc cttttctgct agttctttca
    22621 atccaaccta tagcgaggaa atcaaaggca tcacttgggg attagaatcc ggcgccactt
    22681 ttcctggctt tatcactgtt ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc
    22741 gattagaaga actaatttgt ttggcagatt tagacggttc gtggtggtgg tggccgtatc
    22801 gcttagaggc tcaacaagga gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg
    22861 catcgggtct ttttgtcagc ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag
    22921 gagtattgca aattgatcct gttgataatt taacgttttc ttggaagaat ttgaagttcg
    22981 gtcaagcctt catttcgatt gaatgtacgc aatccgagtt gagtatttca aatttgtcag
    23041 aaaagccatt gaaaataagt gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga
    23101 ttcacattca aaggagaaaa agatgagtat actaaaaaat attacccaat ttaaaaaaat
    23161 ccagagtcga gcaatcactc ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt
    23221 gccaagcgag ctaggaccaa gccgcaaagg tcgtggcgca attccgctga cttctggtga
    23281 agaaactgtg attgcggata ttactggaac gggaataatt cgtcatatgt ggatgacgat
    23341 tcgtgatcga acagaatttg gcagttttgt tttacgtgat ttaatcttaa gaatttattg
    23401 ggatgggtcg gatagccctg ctgttgaaag tcctttaggg gatttctttt gtaatgggtt
    23461 tggagagcgt tgcgaggtga actcgttgcc gatagtggtt aatcctacag ggggctttaa
    23521 ttcctatttc gaaatgcctt tccaaacatc cgctaaaatt acgatcgaga atttacatcc
    23581 gaaggatatg tcctcatttt tttatacgat aaattacgct ttggtggacg aattggaaga
    23641 aaatagccta tattttcacg caaattggca tcgggaacgc attaccaaat tacaggagga
    23701 ttataagtta attgatcgat taaagggtca aggctattac gtgggtactt accttgcgct
    23761 tacctgccta gaacgttatt ggtggggcga aggcgagttt aaattctaca ttgatgggga
    23821 tagtgagtat ccgaccgtaa cctcaacagg ttcagaagat tattttggcg gggcgtgggc
    23881 ctttcatgag tacgatgagt tcggacgtcc acatgcgaaa aactattcaa cagccttttt
    23941 agggtatcct taccaatcaa atagagatgc cacaagagat cgttttgaaa cgggaaaatt
    24001 gaatgccgtt catgcctttg gtgacgacgc aatgccacga catggtcttt atcgttggca
    24061 tcttcaagac cccatctgtt ttaccgaaga tttatcggtc actttacaac aaatcggcaa
    24121 cgatgatgtt cgcttatttg agcggcaaga cgatgttgct agtgtcgctt actggtattc
    24181 aacagaagct tcaggaaata ctaaacgtca tttcactgat cgagaacgct tgccgcgtta
    24241 atgaagtgtt tttcacatat tcgaagggag gaacctatga tgatatatgg ggcaattgaa
    24301 gctggaggga ctaaatttgt ctgtgcagtc ggtactgagg aattaacgat tttggaaaga
    24361 gtcagttttc ccacaacaac tccagttgaa acaatgaagc aggtcattga tttttttaaa
    24421 ccttttaagg aacaattggc aggcatcggg gtcggctgct ttggtccaat tgatattcaa
    24481 tgcaaatcaa cgagtttcgg tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc
    24541 aattttgtag gaacgctaaa gcaatatttc aatattccga ttgaatggac aacagatgtc
    24601 aatgcggctt gctatggtga atatgttgct ggttctggca aagggttagc aggtgttgca
    24661 tacttcactg tgggaacagg tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa
    24721 gggttcagtc atccggaaat ggggcatatg ttgatcaagc gacatccaaa ggacaatttt
    24781 tcaggaaact gtcccttcca ccaagattgt ttagagggga tggcagcagg accagcgatt
    24841 gaaaaacggt tgggtgtaaa aggccagaat ttattggctg acgattcgtt ttggcagatt
    24901 gaagcctttt accttgccca atgtgcatac aatacaacac tgatgttttc gccagataga
    24961 attatttttg gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt tcaggataaa
    25021 tttgttgagc ttataaatgg atatgttgaa ataccaccaa ttgatagcta cattattacg
    25081 cccgaattag gtgataatgc cggaataatc ggcggattag cattggccag aaaagcggta
    25141 agaaataagc aaccataatt aatgaatgtt tcattgatac tcatgtcaaa agtatttaac
    25201 cgagtaatca aatgatcaaa tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc
    25261 tctaagtcaa atcgtgttgt tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct
    25321 tttttctttt tgttgtgcgc tgtatacctt gaggatattc actggtggcg tacacttaaa
    25381 tagtaatata ttgtgttact ttatgtctgt ggtaatagtt atagttgctt accaatgtat
    25441 gttcattttt cgaaatgttg acttcataat ccttggctta atgtttgaat ttttttggtg
    25501 atagattttt tcagccgtat aaaataaatc gtaattctat tatagagagt tgtgattata
    25561 aaaagatagc aagaaaaaaa aagaaagttt ctata
//
LOCUS       DAFCHQ010000038        25253 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000038 DAFCHQ010000000
VERSION     DAFCHQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.038"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            237..1877
                     /locus_tag="LNC71_001575"
     CDS             237..1877
                     /locus_tag="LNC71_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBK6466648.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="LNC71_001576"
     CDS             2031..3353
                     /locus_tag="LNC71_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBK6466649.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="LNC71_001577"
     CDS             complement(3415..3846)
                     /locus_tag="LNC71_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466650.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="LNC71_001578"
     CDS             complement(3910..4746)
                     /locus_tag="LNC71_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBK6466651.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="LNC71_001579"
     CDS             complement(4936..5238)
                     /locus_tag="LNC71_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBK6466652.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="LNC71_001580"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="LNC71_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBK6466653.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="LNC71_001581"
     CDS             complement(5692..5958)
                     /locus_tag="LNC71_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBK6466654.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="LNC71_001582"
     CDS             complement(6212..6937)
                     /locus_tag="LNC71_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBK6466655.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="LNC71_001583"
     tRNA            complement(7303..7373)
                     /locus_tag="LNC71_001583"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="LNC71_001584"
     CDS             7490..8242
                     /locus_tag="LNC71_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK6466656.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="LNC71_001585"
     CDS             8258..8737
                     /locus_tag="LNC71_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBK6466657.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="LNC71_001586"
     CDS             complement(8988..10568)
                     /locus_tag="LNC71_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBK6466658.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="LNC71_001587"
     CDS             complement(10561..11304)
                     /locus_tag="LNC71_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466659.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="LNC71_001588"
     CDS             complement(11480..12256)
                     /locus_tag="LNC71_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6466660.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="LNC71_001589"
     CDS             complement(12504..13340)
                     /locus_tag="LNC71_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBK6466661.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="LNC71_001590"
     CDS             complement(13351..14280)
                     /locus_tag="LNC71_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBK6466662.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="LNC71_001591"
     CDS             14472..14825
                     /locus_tag="LNC71_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBK6466663.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="LNC71_001592"
     CDS             complement(14899..15555)
                     /locus_tag="LNC71_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK6466664.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="LNC71_001593"
     CDS             15781..17715
                     /locus_tag="LNC71_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBK6466665.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="LNC71_001594"
     CDS             complement(17801..19024)
                     /locus_tag="LNC71_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6466666.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="LNC71_001595"
     CDS             complement(19018..20916)
                     /locus_tag="LNC71_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBK6466667.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="LNC71_001596"
     CDS             complement(20910..22028)
                     /locus_tag="LNC71_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBK6466668.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="LNC71_001597"
     CDS             complement(22111..22944)
                     /locus_tag="LNC71_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6466669.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="LNC71_001598"
     CDS             complement(22937..23854)
                     /locus_tag="LNC71_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6466670.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="LNC71_001599"
     CDS             complement(24081..25205)
                     /locus_tag="LNC71_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6466671.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7782 a         5041 c         4300 g         8130 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca ctt
//
LOCUS       DAFCHQ010000039        24338 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000039 DAFCHQ010000000
VERSION     DAFCHQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.039"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..2478)
                     /locus_tag="LNC71_001600"
     CDS             complement(133..2478)
                     /locus_tag="LNC71_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBK6466672.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="LNC71_001601"
     CDS             2757..3659
                     /locus_tag="LNC71_001601"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBK6466673.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="LNC71_001602"
     CDS             3870..5645
                     /locus_tag="LNC71_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBK6466674.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7297)
                     /locus_tag="LNC71_001603"
     CDS             complement(5711..7297)
                     /locus_tag="LNC71_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBK6466675.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7548..7772
                     /locus_tag="LNC71_001604"
     CDS             7548..7772
                     /locus_tag="LNC71_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBK6466676.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7914..9665
                     /locus_tag="LNC71_001605"
     CDS             7914..9665
                     /locus_tag="LNC71_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6466677.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9665..11449
                     /locus_tag="LNC71_001606"
     CDS             9665..11449
                     /locus_tag="LNC71_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466678.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11784..12636
                     /locus_tag="LNC71_001607"
                     /pseudo
     CDS             11784..12636
                     /locus_tag="LNC71_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12733..14619
                     /locus_tag="LNC71_001608"
     CDS             12733..14619
                     /locus_tag="LNC71_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6466679.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14660..15562)
                     /locus_tag="LNC71_001609"
     CDS             complement(14660..15562)
                     /locus_tag="LNC71_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6466680.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15792..17195
                     /locus_tag="LNC71_001610"
     CDS             15792..17195
                     /locus_tag="LNC71_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6466681.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17192..18628
                     /locus_tag="LNC71_001611"
     CDS             17192..18628
                     /locus_tag="LNC71_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBK6466682.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18773..19699
                     /gene="trxB"
                     /locus_tag="LNC71_001612"
     CDS             18773..19699
                     /gene="trxB"
                     /locus_tag="LNC71_001612"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBK6466683.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19912..21639
                     /locus_tag="LNC71_001613"
     CDS             19912..21639
                     /locus_tag="LNC71_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBK6466684.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21655..22005
                     /locus_tag="LNC71_001614"
     CDS             21655..22005
                     /locus_tag="LNC71_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBK6466685.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22099..22959)
                     /locus_tag="LNC71_001615"
     CDS             complement(22099..22959)
                     /locus_tag="LNC71_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBK6466686.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23051..23479
                     /locus_tag="LNC71_001616"
     CDS             23051..23479
                     /locus_tag="LNC71_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6466687.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23641..23817
                     /locus_tag="LNC71_001617"
     CDS             23641..23817
                     /locus_tag="LNC71_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBK6466688.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23844..24290
                     /locus_tag="LNC71_001618"
     CDS             23844..24290
                     /locus_tag="LNC71_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBK6466689.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5187 g         7090 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     6781 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     6841 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     6901 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     6961 tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     7021 tatactgtaa aagcatagca attagactag acatcaaaat aactgacatc aataaatatt
     7081 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     7141 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt tttccctttg
     7201 gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     7261 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     7321 tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaagatt cggtatgcct
     7381 aatttttttt cgtggtgtgt cttgctggtt aatgaaagaa attttgttct tctctaaaaa
     7441 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     7501 aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg gtatcaacag
     7561 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     7621 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     7681 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
     7741 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt tttgcgggct
     7801 gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact attcagcaaa
     7861 aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt aaaatgtcga
     7921 tttttagaaa attaggttgg ttttttcgtc aggaaaagaa acattattta attgggattt
     7981 tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
     8041 ttgatgagat cgccgataag aatttatcaa taaagatcat ttcctggtgg atcttattat
     8101 tgatccttgc agctttctta caatatcttt ttcgttatat ctggcggacg aatatttggg
     8161 gaagtgcagc tcggttggag agagaacttc ggcagcagct attcaaacat tttacagaaa
     8221 tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat gcaacgaatg
     8281 atttgaatgc aatccagaat gtagcaggag ctggaatctt gacctttgca gattcagtga
     8341 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
     8401 ttgcgctgat tcctctgcct ttgctagccg ttacttcacg agtgctgggg tcaaaactcc
     8461 acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
     8521 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctcgcggatt
     8581 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
     8641 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
     8701 gtggaaagta tgtcttggaa ggcacgatct caatcgggca gatggtctca tttattagct
     8761 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
     8821 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg
     8881 aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
     8941 ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgcg atccaagaag
     9001 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacaatc atcaaattac
     9061 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
     9121 aatattcatt gaatgcttta ttaggatcaa tcggttatgt gcctcaggat cacttcctgt
     9181 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
     9241 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg ccaaaaggtt
     9301 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa cagaggattt
     9361 cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat gcactttctg
     9421 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
     9481 aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa gaaatcattg
     9541 ttgtcgatca agggaaaatc attgaacgag gaacccacca cgagctgctt gcattaggtg
     9601 gctggtatga acgaatgtgg gaaaaacagc aattagaagc gaagatagaa ggaggcgagg
     9661 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
     9721 gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
     9781 tatcttattt gcgtttgccc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
     9841 ttttatggat gatcatctgg ctaaacagtc agctacgaca caagttattt tgttttttgc
     9901 tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
     9961 gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat ttgaaaagct
    10021 gcatacacta ggtatgcgtt attttgacca gacaccggca ggatcgaccg tttctcgagt
    10081 gacgaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg tattgactgg
    10141 gatctttgca gtcgtttctt ctttcgttgc catgtttcaa atcaatggag aaattgcctt
    10201 gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc agaaattcag
    10261 ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga
    10321 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    10381 atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    10441 gattctgtta agtccaatca tcaattttct ctatgcattg gcgattgcgt tggcgttgac
    10501 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    10561 gacctatgtc caggaatttt ttaatccaat gtctcaaatg atggactttt tgagcacctt
    10621 tacagatggg atggttgccg gtagtcgtat tttgaaaatc atggatacag aagaatatgc
    10681 accgcaacaa aatcctggag cagatcaagc gattacttca ggtaagatcg agtttcgcaa
    10741 tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt ttgtcgctaa
    10801 tccgggtgag accgttgcat tagtggggca caccggaagc ggaaaaagct cgatcatcaa
    10861 cgtattgatg cggttttacg aattttatga aggccaaatc ttgattgatg atcatgacat
    10921 tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc aagatgcctt
    10981 cttgttttat ggagatattg caggaaatat ccggatgatg aatccagcta tcacagacga
    11041 acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg aattaccaaa
    11101 aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagtggac aacgacagct
    11161 gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg atgaagcaac
    11221 agcaaatatc gatactgaaa ccgaaggatt gatccaagaa ggattggcaa aaatgcgaca
    11281 aggacggaca acgatagcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    11341 tttagtttta gacaaaggtc atatagtaga gcgtgggaat catgaaacac ttctagcgca
    11401 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagaggttgt
    11461 gacagtagca tagtagcatt ttgccctaag cgctagagtg aaaataaaac agagcttcgc
    11521 agattcttca tctgtgagcg ttagtggcgt agtcacccga tttaaaaaag acgacgacag
    11581 ctagtcaaaa gcggctaata accactaaga aagcgttgac aaaagcgttt gctttcgata
    11641 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
    11701 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta
    11761 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
    11821 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
    11881 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
    11941 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
    12001 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
    12061 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
    12121 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
    12181 ggaaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
    12241 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
    12301 acctacctgt tcactataac aatacaggat attctaaata tcgtccgcta ttactataag
    12361 attacatttg atgaagattc agtccatttt tttcgattcg tcacacactt acgttttttt
    12421 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgttt
    12481 caggttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    12541 ttagccgaga cgttcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
    12601 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaaggagg ataaattaac
    12661 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    12721 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    12781 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    12841 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    12901 cgagcgggag ggcagtatca agttgtcatc gggaatcatg tgccagtcgt gtttgaagag
    12961 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    13021 tttgatatgc ttattgatgg aatatcgggc tgtttccaac cattccttgc tatcctagct
    13081 gctgacggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    13141 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    13201 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    13261 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    13321 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    13381 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    13441 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    13501 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    13561 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    13621 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    13681 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    13741 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    13801 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    13861 attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa aaaaatattt
    13921 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    13981 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    14041 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    14101 gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag acagaaaaga
    14161 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact gaatgcagca
    14221 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    14281 ggagaagtac gtgccccttt tgacggaaca gtcatgtccc tgtttccgac gaaacatgca
    14341 atcgggatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt ggatactgtt
    14401 cagttggaag ggaaatattt tgaaagcttg gtgcagcaag gagaaagagt gaaacaaggc
    14461 gatctgttgc ttcgcttcga caaaaagaaa atcgaagagg aaggatacag tttagaaaca
    14521 cctgtgattg tcatcaatta catggattat ttggatattt tggaaaatca aggggaaacg
    14581 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga
    14641 ctaggcttaa ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca
    14701 tgatggctga gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta
    14761 aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa
    14821 tcgatgtatt gatcgtaaaa ttaccaattt tcgaatacag tcgttcgcgt gtcgtaagtc
    14881 gcaaagcggc tgtactcatt gaagatagcg ttgaatcacc gatactggtc aaagctaaaa
    14941 ttggcacatg attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg
    15001 tataagaaat cagaatccca cacatatcac ttgtgcaatt gtacgcttca tagaacgatt
    15061 caccaaaagt ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat
    15121 cttgagaaat caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca
    15181 ttagttgttt ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg
    15241 tcatctgttc taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa
    15301 aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa
    15361 aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg
    15421 tggacgggtg gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg
    15481 gagaatcgct taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt
    15541 tgattttttc acgtaataac atgtagatac cttcttcttg tttttttacg taaatcataa
    15601 cttttttgaa aatgatttgt aaacggattt atgttatata gcttaataaa gacatttttt
    15661 gacaagatat ttacaaaaat ccagaagaaa acactaactt ttgtaaatgt tactggaaag
    15721 aaacgaattt ctattgtttg ttttgtttac aaaaaagata ataaaagtgt aaaaagtaaa
    15781 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
    15841 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
    15901 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgtcagtcag atgtctcagc
    15961 gtatgtggga tgcttctccg tatgaagtca aaatctatgc aactctggat cgggaagaag
    16021 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
    16081 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
    16141 gcgggatatt caaagctttt cgtaccgttc cagtggtttt ggcaatcgtt gaagacatga
    16201 agcgtttatg tccaaatgct tggctgatta atttcgcgaa cccaagcggg atggtgacag
    16261 aagcagttat cagatatggc aaatgggata aagtgatcgg attatgcaat gtgccagtaa
    16321 tggcgatgat gacagaacca acaatgcttg gcaagaaaaa agaagagctg atctacaagt
    16381 ttgccggtct aaatcacttc cattggcaca aagtagcaga taataaagga aatgaccgca
    16441 cggcagaatt gattgatcgt ttatacgatg aagacaatgg attgccgaaa aatatctttg
    16501 acatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
    16561 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
    16621 gtacaagagc gcagcaagtg aaagaaacag aagcagagct gtttgaatta tacaaagatc
    16681 cagaattgga tcataaacct gaacaattgc aaaaacgagg tggtgcttat tattcagatg
    16741 ctgcctgtga aacgattgct tcgatctatg gaaataaaga aacacagatc gtggtatcta
    16801 caaagaatga tggagctgtt cctgatctgc cggcagattg tgtagtagaa gtcactgcct
    16861 atgttggcgc acaaggcgca cgaaatgtag cgtttggttc ccttcctgct gctgaaagag
    16921 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagcc gtgactggtg
    16981 attatggaac agctttgcaa gcgtttacga tcaatccgct tatacgatca ggaaagacag
    17041 caaaacggat catggatgag ttgtttattg cacatcagca gtatttaccg caattcaagg
    17101 aaacaatcag ccggttaaaa gcagaaggaa tcgaagtcca agatgaactg gcaagagaat
    17161 tatcaagcaa tacacatgta atggaggaag tatgatggga ttcagaaaag attttttatg
    17221 gggcggagct acagcagcca atcaatgcga gggcggttat gatgaaggcg gacgtgggtt
    17281 agcgaacgta gatgttgtgc caatcggaga agaccgtttt cctgtcatta ctggaaaaaa
    17341 gaaaatgttt gaatttgatg aaaatcattt ttatccagca aaaattgcga tcgatatgta
    17401 tcatcattat aaggaagata ttgctttatt tgcagaaatg gggttcaaga cttatcggat
    17461 gtccattgct tggagtcgta tttttccaat gggcgatgaa aaagagccaa atgaatctgg
    17521 gttgaaattt tatgaggatg tctttaaaga atgtcaaaag tacgggatcg agccgcttgt
    17581 gacgattaca cattttgatt gtccgatgca tttgatcaaa gaatatggtg cgtggcgttc
    17641 cagagaactc gttggttttt atgaaaatct gtgccgagtc atcttcaatc gctataaagg
    17701 acttgtcaaa tattggttga cattcaatga gatcaatatg atccttcatg caccattcat
    17761 gggcgcaggg ctgtattttg aagaaggaga aaatgaagaa caaatcaaat atcaagcggc
    17821 acatcatgag ttggttgcaa gtgccatcgc aacgaagatc gctcatgaag tagatccgga
    17881 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
    17941 agatgtttgg gcttctagaa agtcagatcg agaaaattat ttcttcattg atgtccagtc
    18001 acgaggagaa tatcccgctt atgcattgaa agaaatggaa cgaaatggca ttcagatcaa
    18061 aatggaacct ggggatgccg cattgctgaa agaacatact gttgatttta tctccttttc
    18121 ttattattct tctcgagttt cgactactga caacgaactc ttggaacaga ctgcaggaaa
    18181 tatctttgct tctgtcaaaa atccatatct ggaagcaagt gaatggggtt ggcaaattga
    18241 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccactatt
    18301 tattgtagaa aacggcttag gtgcagtcga tacgcccgat gagaatggtt atgtggaaga
    18361 tgattatcga atcgactatc tggcagcaca tatccaggca atgaaagatg cagtagaatt
    18421 agatggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
    18481 tacaggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgaca acgaaggaaa
    18541 tggcacatta gcccgttcga agaagaaatc attcaactgg tataaaaaag taattgattc
    18601 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
    18661 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
    18721 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
    18781 tgtaatcgtt attggcgcag ggcctgctgg gatgactgct gctttatatg cttcacgttc
    18841 aaacttatcc gtattgatga tcgaacaagg cgctcctggc ggccaaatga acaatacggc
    18901 agaaatcgaa aactatcctg gtttcgattc aatcatgggt cctgatctag cttataagat
    18961 gtatgaaggt gtttcaagat ttgggacaga aaatgcttat ggaatcgtgc aagatatcaa
    19021 ggatcacggc agccacaaag aagttatttg tgaagataaa acttacgaag gaaaaacagt
    19081 cattatcgca acaggctgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
    19141 tggacgtggc gtttcttact gtgcagtttg tgatggtgca tttttccgta acaaacgtct
    19201 agtagtgatc ggaggaggag actcagcagt agaagaagcc atctatttga cacaatttgc
    19261 ttctgaagta gtcatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
    19321 ccgtgcattt gcgaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
    19381 tggaaatgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaactg gcaatgagtt
    19441 tgatttggca gcagacggag tattcatcta tgtaggattg gatccattaa ctgcaccatt
    19501 tagagaagct gggttgacga acgaagccgg ctggattcca acagacaacg aaatgaagac
    19561 gaatatacca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
    19621 tactgctgtt ggtgatggtg ggattgctgg acaacaagtg tataaatact tggaatcttt
    19681 agaagaaaca actgcataag tttaaaaaga gtgaaagaac ggtttcaatt gttcttttac
    19741 tctttttttt gcgtgaaatc tactgaatcc gatttatgcg aatacttgta accgtgcttg
    19801 ataaaagaat cttttcttga tttaagcgtt ttttggtcct catactttca tatctaataa
    19861 gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg
    19921 gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa
    19981 ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa
    20041 ttcggtactg caggcatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat
    20101 acggtaagac aagcaacaga aggacttgca cgtttcatgg atacacaagg tgaagaaaca
    20161 aaacgccgag gtgtcgctat cgcttatgac tcacgccatc aatcaccaga atttgccatg
    20221 gaagcagcca aaacattagc acatcacaat atcccttctt atgtattcga aagtttacgt
    20281 ccaacaccag agctttcttt tgcagtccgt catttgaaga cgtttgcggg aatcatggta
    20341 actgcatccc acaatccagc agcttacaac ggatataaag tctacggaga agatggggga
    20401 caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg
    20461 ttaaaagttg aagtattatc tgacgacgaa gctgaacata gcggattgat cacgatcatc
    20521 ggagaagaag tggatgccgc ttatctagaa gaaatcaaag cagtaacgat tgatcatgaa
    20581 ttggtggaaa caatgggtaa agatttgaag cttgtgtaca caccacttca cggaacagga
    20641 aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttcca
    20701 gaacaagctg tcgcagaccc ggatttcact acagtcaaat caccgaatcc agaagaacac
    20761 tctgctttcg agtatgcgat gcgacttggc gagaaagaag gcgctgactt actgattgca
    20821 acggatcctg atgcagaccg tttaggcgca gccgttcgtt tgccaaatgg ctcctatgaa
    20881 gtattgacag gaaaccaaat cggagccctg tttgctcaat atattttgga agcacaccaa
    20941 tcgcgaggca ctttacctga aaatgcagcg gtattgaaat caatcgtttc tagcgaattg
    21001 cctacagcaa ttgcggaaca ctatggggct accatgttca atgtattgac aggattcaaa
    21061 ttcattgctg aaaaaattca acaatttgaa gaagatcatt cccatacttt tatgttcgga
    21121 ttcgaagaaa gttacggtta tttagttaaa cctttcgttc gcgacaaaga cgcgattcaa
    21181 gcattgctct tattagcaga agtagcagct tactataaaa aacaaggcaa aacattgtat
    21241 gacggcttgc aaacgatttt tgaaacatat ggctactacg cagaaaaaac gatttctgtg
    21301 acaatgagcg gacaagaagg ctctgcaaaa atctctgctt tgatggaaaa attccgtgaa
    21361 gaagcaccga cagaatttgc cggagcaaaa gtcatccaaa cagaagactt caaagagttg
    21421 acgagaacag accgtgaagg aaacgtagaa aaactaacga caccgccatc tgacgtattg
    21481 aagtatacgc tggaagaaaa tggctggatc gctatccgtc cttcaggaac tgagccgaaa
    21541 atcaaatttt atatcggtgt aaaaggaaac tcttcagaag acgcagctga aaaagtagcc
    21601 gcttatgaag cgattatcaa aaaaatgaca gaagaataaa aaaacttgag actgatggaa
    21661 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    21721 acgcaaaaaa caaaagtaga gaaagtcagc caactattca aaatgttgag tgaccccact
    21781 cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    21841 gcagtagaaa tggaacaatc tgctgtatcg catcaactgc gtttattaag agaaaaccat
    21901 gtcgtcaagt cccatcgtga gggaaaagcg attttgtata gtcttgatga ttctcacgta
    21961 ctggatattt tgaaccagac gctcaaacat gtggaacatc aataacgata ggtgcaaata
    22021 gagacaaact ggactgtatc aacgaatctt cttcgctgac acagtccagt ttttttaaga
    22081 aaaagacaag aaaaactgtt agtctgtttc ttcggtggtt tcagttccat aataactatg
    22141 atcttctagg tctaaagcat tcaaaataag tgaagctgtt tcttcgatag ataaagaagc
    22201 gacattgatt actacgcagc ccaatttttc ataaagatca ttcgcaaaat cgagttcagc
    22261 tcgtattttt tcgatatcgg aataagcagt atcaggattt aatccgtaag agcgcatccg
    22321 ttctttacgg atattgttta aaatatctgg gttattcgtc agtcccacga tttttttagg
    22381 atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta gattggcaac
    22441 ctttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc tggatacgcc
    22501 tagcaaaacg acatctgctt ctagaaaccc acgaggatct ttcccgtcat cgtattttac
    22561 agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat gaagggcgcc
    22621 aggttgccga atgggcttga taccagttaa gcgctcgatt tctgatatcg gcggagtcat
    22681 cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgcgtcatct cgatcaattc
    22741 ttgattgatc aatgtgtgta ggacaagggc tttttctttt agtgcatctt gcagtgcctg
    22801 catcaatgtt ttctcatctg tcacaaatgt tctgcggaat aaattgaact caacggtagg
    22861 atattgggcc atagtggctt gcgctaattt tgtcgcagtt tctccagcag agtcagagat
    22921 gacaaaaaaa gtaatgattt ctgtagaatc ggaagacatg gataaaacct cacttgcatt
    22981 ttttttatgt ttattatacc atactctaga atgtgtactt cgtaatcatt ttgatttgga
    23041 ggaataagtc atgaaaaaac agacattgat tgaacaatca ctagaaacat tgaaaaataa
    23101 cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga agcgcaatcg
    23161 ctacgtatcc gctaaagaag tttttgacta tatgaatcag gaattcaaag gagtaagtta
    23221 cgatacgatt tatcgaaatc tccatgattt tgcagaaatc gatttactgg aagaaaccga
    23281 tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa ggggaaatcg gccatcacca
    23341 tcactttatc tgtacggtgt gcggaacaac gaaagaaatc catatgtgtc caatgaatta
    23401 tttcgaagaa cagctcggtg gatgtacgat cgaagggcat agattcgaga ttttcggaag
    23461 atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt aaaaaaaagc
    23521 aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca gtcttgtttt
    23581 ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga gggaattaac
    23641 atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg tcgcttcaaa
    23701 cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt ctacgaaaaa
    23761 ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa attctagttc
    23821 tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat gacatgaaaa
    23881 cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc aagtcatcta
    23941 tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa ttaacggtgt
    24001 tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa gcaggacgtg
    24061 acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt cgaaaaatac atgccggaac
    24121 aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcacacaa actggtgctt
    24181 catcaatgaa agaatttggc aaagtcatgg gtgcgatcat gcctaaagtc aaaggaaaag
    24241 cagatggaaa ccaagtaaat gccattgtga aagaactatt acaatcttaa ttttatcatt
    24301 taaaggctct tcgtcaatga gtgttggtga agaaattc
//
LOCUS       DAFCHQ010000040        24258 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000040 DAFCHQ010000000
VERSION     DAFCHQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.040"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="LNC71_001619"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="LNC71_001619"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBK6466690.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="LNC71_001620"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="LNC71_001620"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBK6466691.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="LNC71_001621"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="LNC71_001621"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBK6466692.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="LNC71_001622"
     CDS             complement(3374..4681)
                     /locus_tag="LNC71_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBK6466693.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="LNC71_001623"
     CDS             complement(4684..5265)
                     /locus_tag="LNC71_001623"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBK6466694.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="LNC71_001624"
     CDS             complement(5837..7129)
                     /locus_tag="LNC71_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6466695.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="LNC71_001625"
     CDS             complement(7126..7749)
                     /locus_tag="LNC71_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466696.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="LNC71_001626"
     CDS             complement(7752..8588)
                     /locus_tag="LNC71_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6466697.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="LNC71_001627"
     CDS             complement(8605..9492)
                     /locus_tag="LNC71_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6466698.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="LNC71_001628"
     CDS             complement(9508..10791)
                     /locus_tag="LNC71_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6466699.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="LNC71_001629"
     CDS             complement(11474..12157)
                     /locus_tag="LNC71_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK6466700.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="LNC71_001630"
     CDS             complement(12175..12792)
                     /locus_tag="LNC71_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6466701.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="LNC71_001631"
     CDS             complement(12802..13626)
                     /locus_tag="LNC71_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBK6466702.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="LNC71_001632"
     CDS             complement(13619..14179)
                     /locus_tag="LNC71_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466703.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="LNC71_001633"
     CDS             14353..15309
                     /locus_tag="LNC71_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6466704.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="LNC71_001634"
     CDS             complement(15614..16570)
                     /locus_tag="LNC71_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBK6466705.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="LNC71_001635"
     CDS             16674..17276
                     /locus_tag="LNC71_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6466706.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="LNC71_001636"
     CDS             complement(17364..18863)
                     /locus_tag="LNC71_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBK6466707.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="LNC71_001637"
     CDS             complement(18879..19403)
                     /locus_tag="LNC71_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBK6466708.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="LNC71_001638"
     CDS             19701..20450
                     /locus_tag="LNC71_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBK6466709.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="LNC71_001639"
     CDS             20441..21226
                     /locus_tag="LNC71_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBK6466710.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="LNC71_001640"
     CDS             21195..21911
                     /locus_tag="LNC71_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBK6466711.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23047)
                     /locus_tag="LNC71_001641"
     CDS             complement(22013..23047)
                     /locus_tag="LNC71_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBK6466712.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23051..24109)
                     /locus_tag="LNC71_001642"
     CDS             complement(23051..24109)
                     /locus_tag="LNC71_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBK6466713.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQSMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7189 a         5222 c         4224 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcgactga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DAFCHQ010000041        23844 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000041 DAFCHQ010000000
VERSION     DAFCHQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.041"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(55..789)
                     /locus_tag="LNC71_001643"
     CDS             complement(55..789)
                     /locus_tag="LNC71_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBK6466714.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="LNC71_001644"
     CDS             1116..1526
                     /locus_tag="LNC71_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBK6466715.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="LNC71_001645"
     CDS             1519..2220
                     /locus_tag="LNC71_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBK6466716.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="LNC71_001646"
     CDS             2220..3806
                     /locus_tag="LNC71_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6466717.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="LNC71_001647"
     CDS             3825..4046
                     /locus_tag="LNC71_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBK6466718.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="LNC71_001648"
     CDS             4043..4804
                     /locus_tag="LNC71_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBK6466719.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="LNC71_001649"
     CDS             5184..5693
                     /locus_tag="LNC71_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBK6466720.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="LNC71_001650"
     CDS             complement(5768..6307)
                     /locus_tag="LNC71_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBK6466721.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="LNC71_001651"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="LNC71_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBK6466722.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="LNC71_001652"
     CDS             7320..8171
                     /locus_tag="LNC71_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBK6466723.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="LNC71_001653"
     CDS             8199..8834
                     /locus_tag="LNC71_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBK6466724.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="LNC71_001654"
     CDS             8854..9627
                     /locus_tag="LNC71_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6466725.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="LNC71_001655"
     CDS             10035..10832
                     /locus_tag="LNC71_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBK6466726.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="LNC71_001656"
     CDS             10810..11223
                     /locus_tag="LNC71_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBK6466727.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="LNC71_001657"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="LNC71_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBK6466728.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="LNC71_001658"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="LNC71_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBK6466729.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="LNC71_001659"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="LNC71_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBK6466730.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="LNC71_001660"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="LNC71_001660"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBK6466731.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="LNC71_001661"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="LNC71_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBK6466732.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="LNC71_001662"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="LNC71_001662"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBK6466733.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="LNC71_001663"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="LNC71_001663"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBK6466734.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="LNC71_001664"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="LNC71_001664"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBK6466735.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="LNC71_001665"
     CDS             21907..22485
                     /locus_tag="LNC71_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6466736.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="LNC71_001666"
     CDS             22537..23019
                     /locus_tag="LNC71_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBK6466737.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="LNC71_001667"
     CDS             23136..23510
                     /locus_tag="LNC71_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466738.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7785 a         4288 c         5122 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgggagcgt caat
//
LOCUS       DAFCHQ010000042        23642 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000042 DAFCHQ010000000
VERSION     DAFCHQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.042"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="LNC71_001668"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="LNC71_001668"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBK6466739.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="LNC71_001669"
     CDS             complement(1171..1866)
                     /locus_tag="LNC71_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBK6466740.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="LNC71_001670"
     CDS             complement(1879..2595)
                     /locus_tag="LNC71_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBK6466741.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="LNC71_001671"
     CDS             2925..3533
                     /locus_tag="LNC71_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBK6466742.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="LNC71_001672"
     CDS             3858..4181
                     /locus_tag="LNC71_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBK6466743.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="LNC71_001673"
     CDS             4363..5733
                     /locus_tag="LNC71_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBK6466744.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="LNC71_001674"
     CDS             complement(5863..7164)
                     /locus_tag="LNC71_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBK6466745.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9476)
                     /locus_tag="LNC71_001675"
     CDS             complement(7518..9476)
                     /locus_tag="LNC71_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBK6466746.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="LNC71_001676"
     CDS             complement(9653..9919)
                     /locus_tag="LNC71_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6466747.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="LNC71_001677"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="LNC71_001677"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBK6466748.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="LNC71_001678"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="LNC71_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBK6466749.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /locus_tag="LNC71_001679"
     CDS             13263..13826
                     /locus_tag="LNC71_001679"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBK6466750.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="LNC71_001680"
     CDS             complement(14079..14600)
                     /locus_tag="LNC71_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBK6466751.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="LNC71_001681"
     CDS             complement(14603..15445)
                     /locus_tag="LNC71_001681"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBK6466752.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="LNC71_001682"
     CDS             complement(15438..16163)
                     /locus_tag="LNC71_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBK6466753.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="LNC71_001683"
     CDS             16339..16635
                     /locus_tag="LNC71_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466754.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17203..18795
                     /locus_tag="LNC71_001684"
     CDS             17203..18795
                     /locus_tag="LNC71_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6466755.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="LNC71_001685"
     CDS             18808..19548
                     /locus_tag="LNC71_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6466756.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="LNC71_001686"
     CDS             19716..21065
                     /locus_tag="LNC71_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6466757.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="LNC71_001687"
     CDS             complement(21193..21783)
                     /locus_tag="LNC71_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBK6466758.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="LNC71_001688"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="LNC71_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK6466759.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6770 a         4761 c         4502 g         7609 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23641 aa
//
LOCUS       DAFCHQ010000043        22950 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000043 DAFCHQ010000000
VERSION     DAFCHQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.043"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..286
                     /locus_tag="LNC71_001689"
     CDS             <1..286
                     /locus_tag="LNC71_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466760.1"
                     /translation="KSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRFI
                     RMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            345..785
                     /locus_tag="LNC71_001690"
     CDS             345..785
                     /locus_tag="LNC71_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466761.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            797..1465
                     /locus_tag="LNC71_001691"
     CDS             797..1465
                     /locus_tag="LNC71_001691"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBK6466762.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1529..2152
                     /locus_tag="LNC71_001692"
     CDS             1529..2152
                     /locus_tag="LNC71_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBK6466763.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2208..2594
                     /locus_tag="LNC71_001693"
     CDS             2208..2594
                     /locus_tag="LNC71_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBK6466764.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2807..4198
                     /locus_tag="LNC71_001694"
     CDS             2807..4198
                     /locus_tag="LNC71_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBK6466765.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4545..5192
                     /locus_tag="LNC71_001695"
     CDS             4545..5192
                     /locus_tag="LNC71_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBK6466766.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    5321..5537
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5585..8230
                     /locus_tag="LNC71_001696"
     CDS             5585..8230
                     /locus_tag="LNC71_001696"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBK6466767.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            8540..9853
                     /locus_tag="LNC71_001697"
     CDS             8540..9853
                     /locus_tag="LNC71_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBK6466768.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            9843..10496
                     /locus_tag="LNC71_001698"
     CDS             9843..10496
                     /locus_tag="LNC71_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6466769.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            10551..11243
                     /gene="radC"
                     /locus_tag="LNC71_001699"
     CDS             10551..11243
                     /gene="radC"
                     /locus_tag="LNC71_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBK6466770.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            11393..11593
                     /locus_tag="LNC71_001700"
     CDS             11393..11593
                     /locus_tag="LNC71_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6466771.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11847..13526)
                     /locus_tag="LNC71_001701"
     CDS             complement(11847..13526)
                     /locus_tag="LNC71_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK6466772.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(13528..13740)
                     /locus_tag="LNC71_001702"
     CDS             complement(13528..13740)
                     /locus_tag="LNC71_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBK6466773.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            14133..15863
                     /gene="efrA"
                     /locus_tag="LNC71_001703"
     CDS             14133..15863
                     /gene="efrA"
                     /locus_tag="LNC71_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBK6466774.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            15860..17620
                     /gene="efrB"
                     /locus_tag="LNC71_001704"
     CDS             15860..17620
                     /gene="efrB"
                     /locus_tag="LNC71_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBK6466775.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            17715..17912
                     /locus_tag="LNC71_001705"
     CDS             17715..17912
                     /locus_tag="LNC71_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466776.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            18065..18550
                     /locus_tag="LNC71_001706"
     CDS             18065..18550
                     /locus_tag="LNC71_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBK6466777.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(18654..19247)
                     /gene="clpP"
                     /locus_tag="LNC71_001707"
     CDS             complement(18654..19247)
                     /gene="clpP"
                     /locus_tag="LNC71_001707"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBK6466778.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            19427..19954
                     /locus_tag="LNC71_001708"
     CDS             19427..19954
                     /locus_tag="LNC71_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466779.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            20045..20782
                     /locus_tag="LNC71_001709"
     CDS             20045..20782
                     /locus_tag="LNC71_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK6466780.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            20786..21703
                     /gene="miaA"
                     /locus_tag="LNC71_001710"
     CDS             20786..21703
                     /gene="miaA"
                     /locus_tag="LNC71_001710"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBK6466781.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            21705..22934
                     /gene="hflX"
                     /locus_tag="LNC71_001711"
     CDS             21705..22934
                     /gene="hflX"
                     /locus_tag="LNC71_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBK6466782.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         7463 a         3944 c         4966 g         6577 t
ORIGIN      
        1 aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt
       61 gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat
      121 ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga
      181 cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag
      241 aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt
      301 ggattttttt gtcataataa atattagatg atggaggaga taatatgatg caaacatggt
      361 tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga
      421 aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc
      481 gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat
      541 ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat
      601 atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg
      661 atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta
      721 aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg
      781 tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt
      841 tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc
      901 agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc
      961 tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg
     1021 gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa
     1081 aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg
     1141 atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt
     1201 ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc
     1261 cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg
     1321 tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat
     1381 ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac
     1441 aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa
     1501 agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac
     1561 attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg
     1621 tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt
     1681 gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa
     1741 agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa
     1801 agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct
     1861 gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt
     1921 tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag
     1981 aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg
     2041 agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt
     2101 ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct
     2161 ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa
     2221 gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag
     2281 aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag
     2341 aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat
     2401 ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg
     2461 tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt
     2521 cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag
     2581 aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt
     2641 ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat
     2701 aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt
     2761 tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat
     2821 cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa
     2881 gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg
     2941 cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg
     3001 gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt
     3061 gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat
     3121 tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat
     3181 tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac
     3241 agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca
     3301 gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac
     3361 aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa
     3421 aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat
     3481 cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat
     3541 gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt
     3601 tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc
     3661 aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac
     3721 ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg
     3781 agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg
     3841 ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa
     3901 tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg
     3961 tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa
     4021 cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg
     4081 tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa
     4141 agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc
     4201 aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat
     4261 agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg
     4321 tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
     4381 tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac
     4441 cgttttattt gtctcacacc cttttccttc ctacttgtaa ccttgctccc gtgttagaaa
     4501 catgttacac taaatatgtg aaattattaa caaggagcgt cgttatgcga gaaaaaacag
     4561 atacactgcc aaaagcaact gcgaaaagat tacctttata tttacgctac ttgaagatgc
     4621 tggatgactc aggtataagt agaatcaaat caaatgagtt cagcgaaatc acccaaattc
     4681 cttctgcaac gatccgcaga gacttctcgc aattaggcga gctaggcaga agcggatatg
     4741 gctatgatgt cccttttctt atcgatgtat tcaataatat ccttaatact aaagaagaaa
     4801 aacgaatcgc attagtagga tatggaaatc ttggcaaagc attgaaacac aacaatttta
     4861 gaagaaatga aaatctaaat atcgtgtgcg tctttgataa tgatccagca ctgatcaatc
     4921 gagtaattga tggagaaatg atctatccga ttgatcgttt cgcggagata gccaaagcaa
     4981 aaaatgtgac agtagcaatc tctacggtac caagtaaata ttcacaatct gctattgatg
     5041 aaattgtcaa aggaaatgta accgctatac ttaattttgc gcctgacaga gtaactgtcc
     5101 cagcttatgt caatgttcaa tacattgatt tgacaaccga gttgcagacg cttatttact
     5161 ttgatgagaa ttactcagaa gttttttctt aggaaaattt gagtaggctt attgatcttg
     5221 cgagaactcg tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg gttttttcac
     5281 gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga agcataacgc tgaacaagag
     5341 gagtaactgg cacattcatt cttagagaga gaactatctg ctgaaaggtt ctcaatgaca
     5401 accagtgaag gtagcttggg agtttctgtc ttgaatggag tagagataga cgagtcgtga
     5461 tcgttaactc acttgagagg caataatgat tgattattgc aatttaggtg gtaccgcgta
     5521 aatcatacgt cctagaatac gaaagtattt taggacgttt tttagtttaa aggaggaaaa
     5581 cagcatgacg gaagaaaata acctatcaac aaaatataat ccgcaagaag ttgaagctgg
     5641 acgttatcag cattggttag aacaagattt gtttaaaccg agcggagata aaaaagcaaa
     5701 accttattca atcgtaatcc cgcccccaaa tgttactgga aaacttcatt tagggcatgc
     5761 ttgggatacg accttgcaag atatgctgat tcgtcaaaag agaatgcaag gatatgatac
     5821 tttatggctg cctggtatgg accatgcagg aatcgcgaca caagcaaaag tagaagcgaa
     5881 gcttagagag caagggattt ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt
     5941 gtgggaatgg aaagaagagt atgcgagcca catccgcgaa cagtgggcta aaatgggctt
     6001 atctttagat tacagtaggg aacgttttac attagatgat ggattatctg aagcagtacg
     6061 taaagttttt gttaccttat acgaaaaagg tttgatttat cgtggtgaat atatcatcaa
     6121 ctgggatcct caagcacgaa cagctctatc tgatatcgaa gttattcata aagatatcga
     6181 aggcgccttt tatcatatga gctatgaact agcagatgga tcaggcgtag tagaaattgc
     6241 gacaacccgt ccagaaacga tgttgggaga tacggcgatt gctgttcatc cagaagacga
     6301 gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct ttagtcaata aacaaatccc
     6361 aatcattgca gatgaatatg tagatatgga attcgggaca ggtgtagtaa aaatcactcc
     6421 agcacatgat ccaaacgact ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt
     6481 tatggatgat gatggtacga tgaatgaact agcaggaaaa tatgctggaa tggaacgttt
     6541 tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga
     6601 tacgatgatc cacagtgtgg gtcattccga gcggacaggt gtcgttgttg aacctcggct
     6661 gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa aaagccattg ataaccaaga
     6721 aacagatcaa gcagtggaat tcttccctcc aagattcaat caaacgtttt tacgttggat
     6781 ggaaaatgtc catgattggg taatctcacg acaattatgg tggggacatc aaattcctgc
     6841 atggtatcac aaagaaacag gtgaaatgta tgtagggatg gaagcaccag cggatagtga
     6901 aaactgggta caagatcctg atgttttaga cacttggttt agttcagctt tgtggccttt
     6961 ctcaacaatg ggctggccag atacggaaag cgaagattat aagcgatact tccctactag
     7021 cactttagta accggttatg acatcatttt cttctgggta agcagaatga ttttccaaag
     7081 cttggaattt actggcgaac gtccatttga gaatgtcttg attcatggat tgattcgtga
     7141 cgaacaaggg cggaagatga gtaaatcatt aggtaatgga atcgatccga tggaagtaat
     7201 cgaaaagtat ggagcagatg ctctccgctg gttcttatcg aatggttctg caccaggaca
     7261 agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg aactttatca acaaaatctg
     7321 gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg accgtagaag agatcgattt
     7381 atctggagaa aaatcagtgg ctgaccggtg gatccttact cgtttgaatg aaacgatcga
     7441 acgagtaact gagctatttg atcgttttga atttggtgaa gcaggtcgac aactgtataa
     7501 ctttatctgg gatgattttt gtgactggta catcgaaatg agtaaagaag tactttatgg
     7561 agatgatgct gtttcaaaac aaaccacccg tagcatctta gtccatacac ttgatcagat
     7621 cctacgttta ttgcatccaa tcatgccatt cgttacagaa gaaatatggg aacatattcc
     7681 tcatcaggga aattctctag tagtggcaga atatcctgtt gtacgtccag agtttgatga
     7741 tgaaacagct tctaaaggga tggaagtatt gaaagaattg attcgagccg ttcgaaatat
     7801 tcgttcagaa gtgaatacac cattgtctaa accaattacg ttgctaatta aaacaaacga
     7861 tgctaaaatc gatcaatttt tagttgaaaa tacaagctat atcgaacgct tctgtaatcc
     7921 cgaagagttg acgatatcaa gtgagatcac tgcgccagat ctagcgatgt cagctgttct
     7981 gacaggtgca gaaatcttct tgccgttagc aggattgatc aatattgaag aagaaatcaa
     8041 acgattagaa aaagaactgg aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact
     8101 ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa
     8161 agaaaaagat tatttagaga agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac
     8221 tattcaataa gaatagaaaa agagagtgtg aaatcagcta gctattacta aaagctagct
     8281 gattgaacat cgttacagga aagagtttga gcctcactct tagcttcgat tcataaggag
     8341 atgtgacata aattttgtcg catctctttt ttagtctact cttattatgt aggagaagga
     8401 gggaggtgga tcatgtatca agagtcaata aagtagttga taaaatcgtt cctctctctg
     8461 tttcttttct ctttgtcata cttttatttt tcttatgtgt tctatttgtt ataataaaag
     8521 agaaatgggg gaattgttga tgacgattga agaagcaata gaatggatcc atagtcgatt
     8581 accatttggt tcacgtcctg gccttgaccg agtcgaagct ttattagaag tggtagggca
     8641 tccagagaga aaagtaaaaa cgatccatat cgctggaaca aacggaaaag gttctactgt
     8701 agcttatttg agaagcctat tagaagaggc tgggctgaca gtaggtacat ttacttcacc
     8761 ttatatcgaa acgttcaatg aacggatcgc aatcaatggc aaaagcattc cagacaatga
     8821 tttgatccag cttgtaaaaa aatatcagcc actgatcaaa caacttgatc aatttgaagc
     8881 agtagctggt attactgaat tcgagacatt aacagcaatg gcatttgatt attttttgat
     8941 gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc ggcttattgg attctaccaa
     9001 tgtagctgta ccaatgctga cgggaataac aacgattgga cttgatcata ccgaaatact
     9061 tggagataca attgaagaaa tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc
     9121 agtagtaaca ggaaatattg ccccagaagc ttttactgtc atccaacaaa cagctaaagc
     9181 aaaaaatgca ccgctttatg tatacgggga aaattataaa ataaactatc tgcatcctga
     9241 tgagatgtgg ggagaagtat ttgaattttt taatgaaata ggaaagatcc ctcaattaac
     9301 cactccgctt ttagggcaac atcaagctga aaatgcagga gtagccatcg aattgtattt
     9361 tgtttactgt caattggaaa agctgccttt ccgagaaaaa gatgttttga agggattgaa
     9421 gaaatctcga tggccaggaa gaatggagcg tttgagtaac gagccattag ttgttttaga
     9481 tggtgcccat aatacgcatg caatgaaccg gcttgttgag aatttgaaaa aagaattcaa
     9541 aggatatcaa atcaacattt tgttttccgc cttacgaact aaaaatgtta gagaaatgct
     9601 agaacgattg cagcaagtac caaatgtcca tatttatttg acgacctttg aacatccaaa
     9661 agccatcgaa ttaagtgaag tatcagattt agcaagtgag agagtatcaa tcgtttcatt
     9721 gtggcaattc ggattagcag atatattgga aaagatgaca aatgaagacg tattagttgt
     9781 gaccggttca ctttatttca tttcagaagt tcgccattta ttaatacaat taggaggaga
     9841 tcatgaagta taatgcagtg atttttgaca tggatggttt gctgttcgac acagagttag
     9901 tgtattacga agccacacaa gtagtagcag acaagatggg ttttccttat gataaagacc
     9961 tgtatttaaa attcttaggt gtttcagatg aagaagtatg ggcgaattat catatgattt
    10021 tctcggaatt tggcaaaaca agtgtacaaa gattcataga tgattcatac caggaaacgc
    10081 ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt
    10141 ttttagaaga acatcagatt cccaaagttg tcgcttccag taaccaacga gcagtcattg
    10201 aacttttatt gaagaagaac aatctgtttg aacgatttga gacgattgtt tctgcagaaa
    10261 atgttcaacg agcaaaacca gacccagaaa ttttcttgtt agctaatcag aagctaggta
    10321 cagcgaagaa agaaacattg attttagaag attcacaaaa tggtatttta gcagcttctg
    10381 cggctgagat cccggtagtc atggtaccag atctgcttcc accatcccaa gaattgatca
    10441 aaaaaacagc tggggttgtt tcttctttac atgaaatccc tgcattgtta aaataaaaat
    10501 ccatctcttt tcgtatctta ttttagataa gatagaaagg agatgttttt ttgggaaaac
    10561 gattgatcaa agaagtacct actagctctt taccaaggga acgtatggaa atttatggcg
    10621 cggaagcttt atcggatcag gagcttttgg caatcttact gcgtaccggt caacaccctt
    10681 atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt tggaggactg gcctctttac
    10741 gacaggctac tttgcatgag ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg
    10801 aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa agagcggctc
    10861 caccggttcg taccagctac gaattggccc agcaattgat tctggaaatg aaagatcaca
    10921 aacaagaaca tttggtttgt atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa
    10981 cagtgttcat cggttcgctg aatcagtcag tcgctcatcc tagagaaatt ttccattatg
    11041 ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa tcatccttct ggtaatgtta
    11101 ttccatcgca gcaggatatg aattttacca aaaggataca aaagtgtgga gaaatgatgg
    11161 ggatcactgt actggaccac ttgatcatcg gacgcaaacg atattttagt ttgagagaag
    11221 aaggaatgat ggaagagaag taagttgctt tcttgcaaaa aaaatgaagc aagtgtacaa
    11281 ttatattgta attttgtttg ggaatagaag ttgaaacatc tgttgcacct ctatcgtaca
    11341 cgagcaatgt tacaatttaa attagtgctt ttggcacgag gaggatatta ttatggaaaa
    11401 cggaacagta aaatggttca acgctgaaaa aggatttgga tttatctcac gtgaagatgg
    11461 aagcgacgta ttcgttcact tctcagctat ccaaggtgac ggattcaaaa cattagaaga
    11521 aggtcaagca gtgactttcg acgttgaaga atcagaccgc ggcccacaag ctgcaaacgt
    11581 tgtaaaagct taattttatc tgtaaatcaa attgatggta gacatcaatt tttacgagag
    11641 aacaaagcga gtaacaaaaa tacctgccac aagagacaat ttctcttgtg gcaggtattt
    11701 ttttgtatga aaaagtaaat aaatttaaga ggctatgaaa gagccacctt gtatcaagga
    11761 ataagaacaa acaaaaatgg cttctcatgt ttttcatttg ttttctctta tattcaggac
    11821 aaggtgctga tttcataacc tcttatttat tcttcatctg gtgtcattac cattggcaaa
    11881 atcattggat gtctttctgt acgttcgaat aagaaaggct gaagcgcgcc agtcatcgct
    11941 tctttcaatt tatattctga acagtcaggt ccattcaaac attctcttaa agcgtggaat
    12001 aagattcgct gagcttcatt gatcatttct cctgattctc gcatgtagac aaatccacga
    12061 gagagaatat ctggaccagc caagatttct tttttcttga tatccactgt cgcgacagct
    12121 aatacaagac cttcttctga aagcaggtga cggtcacgta aaacgacatt accaatatca
    12181 ccgatcccgc taccatcgat atacacatct ccagccacaa aatgcccagc aggacgcgca
    12241 gaatcgctag ttaacgctaa aacatcgcca ttttccatga tgaagcagtt ttctgcagga
    12301 acacctacat cttgtgctag agaagcatgt actttcaaca tgcggaattc tccgtggaca
    12361 ggaataaaat attttggctt catcaaacga agcattaatt tttgttcttc ttgtccaccg
    12421 tgaccggaag tatggatgtt attgattttt ccgtgaatca cttctgcacc agcttcagac
    12481 agcaaattga tcaaatgatt gacacttgtt gtattaccag gaataggtga acttgaaaag
    12541 atcactgtat cgtctggctg gatttggatt tgacggtgag taccattcgc gatgcggctc
    12601 aacgcggcca tcgcttctcc ttgagaacct gtacatagga tcatcgtttc attggcaggc
    12661 aagcgattga tttcgtgtgc atctacaaat gtgccgtctg gaactttgat atatcctaaa
    12721 cgttgaccgt tgacgatggc attttccatg cttcgtccga aaacagcgat tttacgacca
    12781 gttgccacag cggcatctgc tgcttgctgc aaacggaaga tgttagaagc aaagctggca
    12841 aaaatgatac gtccttcgat ttttgaaaag atcttcaaaa tagaatgccc aatcgttttt
    12901 tctgatttag taaatgttgg aatctcagca tttgtactat cagataaaag gcacaagact
    12961 ccttcttccc cgatacgagc cattttatgc aagtttgctg gttcgccaac aggagtgaaa
    13021 tcgaatttga agtctccagt cgctacgata tttccagaag gtgttttcac cacgatccct
    13081 aatgcatctg ggatactatg ggtcgttctg aaaaagctga cagaagtctt tctaaaacgg
    13141 atgatcgtat cttcattgat ctcatggagc tctgcatctc gaagcaagcc atgctcatct
    13201 aatttgtttg tgatcaacgc taatgcgaga ggaccagcat agataggaat atttgcttgt
    13261 ttcagcaagt agggtattcc gccgatgtgg tcttcgtgcc cgtgagtaat caccaaggct
    13321 tttactttgt gtaagttttg tatgatgtag ctgtagtcgg gaatgacata gtcaattcca
    13381 agtaaatcgt cttccgggaa tttgatcccg gcatcaatca aaatgatttc atcttggaat
    13441 tgtaccccat acgtattttt cccgatttca cctaatccac caatagcaaa gacgcctgtc
    13501 tcgttatttt tgatggaaag tttcatatta aaactccact aatgagaaat cagcattttc
    13561 tttttcgtac tctagatgac ttccttctag aagttgaacg aattcaatat tgtatggggt
    13621 attttcttcc acacgttggc gagcgtctac atcgcttttt gcctcaatgt aaagagattt
    13681 tgtttcttcc cgtttagggt tgcgtacttt tgtttcttga taataaactt tatagatcat
    13741 tgttttgtct cctttatctg cgtaaagcag tgatttagct agttgatttt tttagacaac
    13801 acaaaacgag gggacaagcc acctggtttt tcatatgctt tgactagaat actggattcg
    13861 agtcaaagag acaagcctcg tttttgctta tctgttcgga tattttttca accgtacctt
    13921 ctgtataaaa aatatacaac aattaattgt tattctagca taaaacagca aataagtata
    13981 gaaatcaact agattgaaag aaaaaatcaa tgtttatcat cccgctataa tcataatgac
    14041 ttttttatga gagaagtgtt ctattaatag gctcgttgtg ctatcatttt tagtaatgaa
    14101 aatgtaacaa aagattaaaa ggagatgaga aagtgaaact aatgtggcat tataccatgc
    14161 gttataagaa attcttgttt ttcaatttca tatgcgtatt tggttttatt ttgattgaat
    14221 taggacttcc tacgatttta gcacgtatga tcgatgtagg aatccgcaat gatgattttg
    14281 attatgtgaa gcaacaaggg atatttatga tcgttattac tgtaatcggt attattttga
    14341 atatcttgtt aggttatttt ggttcgagga ttacgactaa tatcgtagca gatattcgag
    14401 atgatttgtt cgaaaagatc caaagttatt cgcatcagga atacgaaaat ctaggcgtat
    14461 cttcattgat cactcgtaca acaaacgatg cttatcagat catgttgttc ctgcaaaata
    14521 ttttacgtat cggttttatg gcaccaatga tgttcattgt cagtttgtac atggtcatgc
    14581 gtacaagtgt gacactcagt ttgtatgtag taggtgcatt acctttacta ttattggcag
    14641 tcgtagcgat tgctaaggtg tctgaaccgt tatctaaaaa gcaacaaaaa aacttagata
    14701 aaatcaacag tatcttgcgg gaaaatcttt caggattgcg tgtgattcgt gcattcgtca
    14761 atgaaaagtt tgaagaaaaa cgttttgctg tagtcaatga gaattatgct tctagttcaa
    14821 aaagcttatt ccgtttgatg gctgttgcac aaccgggttt ttatttctta tttaatatcg
    14881 taatggtatt gatcatctgg agcggggcag tgcaaatcga tgcaggcgag cttcgtgtag
    14941 gagatttgat tgcgtttatc gagtatatat tccatgcctt gttctcattc atgttatttg
    15001 ctagtgtgtt catgatgtat ccaagagcag ctgtttcagc tggacgtatc caagaagccc
    15061 tagatgccaa accttcgatt gtagaagatc caaatggtat atctgataca aaaactaaag
    15121 ggtatatcga atttcaaaat gtaacatttg cttatccagg ccattcgcaa gagccagtga
    15181 tacgaaatgt cagttttaca gcctctccag gagagaccgt tgcttttatt ggaagtaccg
    15241 gtagcggaaa atctacgttg atccaattga tccctcgctt ttatgatgtg agtgaaggaa
    15301 agatcttgat cgatggagta gacgtgagag attatcggct aagcaagttg cgagataaga
    15361 ttgggtttat tccgcaaaaa gcgctgctat ttactggaac gattgcagat aatttgcgat
    15421 acgggaaaga agatgctacg caagaagaaa tggaacgtgc agctgagatt gctcaagctt
    15481 cagaattcat ttctcgaaaa ccagatggat ttaatgaaca tttatcagaa ggtggttcca
    15541 atttttcagg tggacaaaaa caacgattgg caatagccag agccatcata cgccgtccgg
    15601 aaatctatat ttttgatgac agcttttctg cattagatta tcaaacggat gcgaagttac
    15661 gtgctcgtct gaaaaaagaa acgacagaat ctactgtatt gatcgtagca caacgtgtgg
    15721 gaacgatcat gcatgcagat aagatcatcg tattaaatga aggagaagtc gtcggtatgg
    15781 ggacgcatcg ggagttgttg gaaaaatgcc cgatttatta tgatattgcc gcttctcaat
    15841 tatcaaagga ggaattagca tgaaaaaaac cttttcttcc ttaaaacgtt tagctcgata
    15901 tatcagaccg tatcgtgaga cttttctttt agtgatccta tttacagttc tgacagtggc
    15961 tttcaacgtg gctttgccat atgttacagg acttcctact acagaaatca gccgtaatat
    16021 tgccaatggc gaatcaatca atttttctta tatattcaaa tgtctcattt ggatcacagt
    16081 tgttggaata gggtattgta tttcgcaatt actttctggt gttttgatga caaatgtcgt
    16141 tcaaagtgcc atgcatgatt tgagaagaga catagaagaa aaaatcaatc gtcttccggt
    16201 ttcatatttc gataaaaacc agcaaggaaa tattttgtct cgtgtgacga atgatgtaga
    16261 tgcagtcagc ggtgctttac aacaagcttt tattggtatc gtaaatgcga tattgggaat
    16321 tacgttggct gcagcaatga tgttttatat ccagccgatg atggcgctga tctctatgat
    16381 catgattcca acatcgatct ggatctcaaa acgagtgatc aatgcttcgc agaaatattt
    16441 tcaaagcatg cagaattctc ttggtgagct aaacggctac gtccaagaaa atatgacagg
    16501 ttttagtgta ttgaaagtat atggacgcga aaaagaaaca ttggaaggat tcaaaaaagt
    16561 taatcacagc ttgaaatact ttgggtttcg tgcagcattc atttctggct tgatgatgcc
    16621 tctagttcaa ttaactgcct atgctactta tattggtatg gctgttctag gaagctttta
    16681 tgttattact ggggcgatcg ttgtcggtca actacaagca ttcatccaat atatttggca
    16741 aatcagtcag ccaatgggga acgtgacaca attgtcttca gttttgcaaa gtgcatcagc
    16801 ggcaacgaaa cgtgtattcg agattttaga tgagccagaa gaaaaagaaa acaaagtaga
    16861 tgcttcactg ccagaaacga ttttaggaga tgtgacattc gatcatgtcg attttgcata
    16921 tgatccgaag aaaccattaa tccaagattt gaattttgaa gtgaaagctg gtcaaaccgt
    16981 agctgtggtc ggtccgaccg gtgccggcaa gacgacattg atcaatctat tgatgcgttt
    17041 ttatgacgta gacaaaggag caataaggat tgatggaatc gacaccaaat caatgagtcg
    17101 cagtgatgtg cgttcgctat ttggtatggt cttgcaagat gcatggcttt atcaaggaac
    17161 gattgcagat aatatccgct tcggaaagct tgatgcgaca gattatgaag tagttgatgc
    17221 tgcgaaaaca gcaaacgtag atcattttat ccgaacgatg ccagatggat atgatatggc
    17281 catcaattct gagggagaca atgtgtcttt aggacaaaaa cagttgctga cgattgcccg
    17341 agcagtggtc tccgatccta aaatcttgat tttagatgaa gcaacaagtt cagtagacac
    17401 ccgtttagaa gcgttgattc aaaaagcaat ggatcgcgtg atggagggac ggacaagttt
    17461 cgtcatcgct caccgtttat cgacaatccg tgatgcagat ctgattctag tgatggatca
    17521 aggtcagatc attgaaaaag gaacgcatga gagtttattg gcacaaggtg gattttatga
    17581 aaaactgtat aacagccaat ttgctgagga agcagaataa gtcatataag aataaaacag
    17641 tttaaaactt attttttgtt ctctattctt gataatggta aagtaaagtt atcaagaaaa
    17701 gtgaggacac ggagatggaa cagtttttaa atgaaaaaat tactgaagcc aaaagagaag
    17761 ttgatcgttt aacgcacgtc tgtcagacga agccttttga ttcagctgat ttttttgaaa
    17821 atgagtttca aatcgaacgc ttggcagcac aaatagaagt gtacgaatta attttaaaaa
    17881 aagtaataga agaaaatata gatgtacaat aaaaacaggt aaaacgtcag ttaccagata
    17941 ctgtaaatag acatctggcc tgagcaggtt ttacctgttt tttttcttat gaaataaata
    18001 tatgctatga tacctttatt caataggatt agtttctaca aaaaatgaaa ggagaaaaat
    18061 aaatatggaa cggtttatag catcaaatgc cacagtgatt ggtgatgtga cattgtcaga
    18121 agatgtgacg atctggtatc aagctgtttt acgcggggac agtaattgga taaaaatagg
    18181 acaaagaacg aatatccaag atggtacaat catccatgtc gatcatgatg cacctgtaga
    18241 tattgcggag aatgtgacgg taggacatca gtgcatgcta catggctgta caatcgaaaa
    18301 aggggcgctg atcgggatgg ggaccacgat attgaatcat gcggttattg gtgagaatag
    18361 tttgattggt gctggatcat tagtaacaga aggaaaagtt attcctccta atgtcttggc
    18421 ttttggtcgc ccagctaaag tgatccgtcc tttgacaaaa gaagaaatac aaaaaaacaa
    18481 agaaaacatc cagcattatg tcgagatagg tcggatgcat gcgcaaggaa aattcaagga
    18541 gcaaagatag gcagtagcat ccataaaata aaaaaggatg gtcagtttga gttgaccatc
    18601 cttttttatt tggtttagat aagagagctt aaaaaagctt cctttgaaac agattaattt
    18661 aatgaagaac tgttttccat gacttcatcg atcaatccat atgcttttgc ttgttcagct
    18721 gtcatatagt tgtcacgatc tgtatctttt tcaatcactt caagcggttg tccagtgcgt
    18781 tcagctaaaa ttttgtttaa gcgttcgcgt gtttgcaaaa tatggcgtgc cgcaatttcg
    18841 atttcagttg cttgcccttg agcgccgcca agaggttgat ggatcatgat ctcagcattt
    18901 ggtaaagcaa atcgtttacc ttttgtacca gctgtcagca agaagcttcc cattgaagca
    18961 gccataccca taacaattgt ttggacatct gctttgacaa agttcattgt gtcgtaaata
    19021 gccatacctg ctgtaactga accaccagga gaattgatat aaagatagat atctttttca
    19081 gaatcttgag cgtctaagaa aagcaattgg gcgatgatcg aattagcaag atcatctgtt
    19141 acttggccgc ttaacatgat gatacggtct ttcaataaac gtgagtagat atcgtaagct
    19201 ctttcacctc gtgaagattg ttcaatgact gtaggaatta aattcataat agagttcctc
    19261 cttgatttcg tttagaaata gtgtaccata atttagtttc taatacctaa aatgtagtat
    19321 acatttttgg tcaaaaaagg tcaaataaaa aacttaggca aattagtcgc ataaacccgc
    19381 tttatggtat gataagcaga gaaacaaaac aaggaggaac aacagattga tagttggtat
    19441 tactggcctt atcggagtgc tgacaggtgc tgtagttgtc tatatcggca tgatactgca
    19501 aatgcgtcac ttggaaaata aagaacagcg tagacgtgaa ttggaattga aagtcaaaga
    19561 aatcgagacg ctgaatcaat taaataaaaa gatcaacgag atcttgcaaa aacggacaac
    19621 gctgatggaa gaatatgtca gctttgatgc atttgatgat tgttatatca cgattgacga
    19681 ttttgcgtat ctccaatcat tttcagcgca aaatagtttt tatctgccaa attatatttt
    19741 agacgagcta tttaagaata tctcccatcg aaaagtgatt ttatctccag atgaaacaat
    19801 gaagattggt ggatatgctt ataaaggcgg acgcgtgatt ttagagaatt ttacggatag
    19861 tctgactgaa atgatcaatg aaaaaaaagc tcagttgaag actatgacgc gccagccgat
    19921 tggtttcttc tcgaaagatg atgtttggag ctgatatttt gaagcattgc cgttgcacat
    19981 gagagcgtgg taatgctttc tttttttctt aatgatacaa tagaactgaa cggagtgaag
    20041 aaagatgtta caaatctatg cccatcgcgg aagcagcggc acccacccag aaaatacgct
    20101 tcctgcattt gcagaagctg tacgagtagg tgcagatggt atcgaactag atgtccatct
    20161 aagcaaagat ggttatttga tcgttatgca cgacgaagaa gtggaccgga cgactaacgg
    20221 taaagggctg attcgagaaa aaacattgga agaactcaaa aaattgaatg cagggggcaa
    20281 attcagcaaa gaatttccag cagctaaagt tcctgaattg agagaagtgt taggactgct
    20341 cctccaaaaa aattaccgag ggatgctaat gattgaaatt aaaactgatc agtatgaata
    20401 tcccggtatc gaagaaaaat tagccgaaac attaaaaagt agagattggc cttttcaaca
    20461 tgcatattgc agctttcatt tggattcttt ggaaaaaatc gcattactgg aaccggagat
    20521 acagctggac ttattgatga gtacttctga taaaaagatc caactagcag cagaaacccc
    20581 ttatatagaa ggagtacatc caaaaaatac ttggttgttc gaacatccag tccaggcagc
    20641 tgattttcca aaagcaattc gtgcatggac gattaacgat gaagaagaaa tggccaaatg
    20701 tcaagaactc aaaatagatg gaataataac tgattttccg gagaaagctc ttcggtggaa
    20761 acaatcaacg aaaagtaggt aaaatatgga aaaagtatta gtgatcgtcg gtccgacagc
    20821 agtcggtaaa acagcgctaa gtgtggaact cgcacaacta tttggaggag agatcatcag
    20881 tggtgattca ttgcaaatct ataaaaatct ggatatcggt acagcaaaaa tcaaagaaga
    20941 agagatgaat aatgttccgc atcatttgat tgatgtgatc gaacctactc aacgttactc
    21001 agccgttgat tttcagcatt caggtcgaca attgattacc gagattgcga accgagggca
    21061 cctacctatc atagctggtg gtacgggtct ctatatccaa tcccttctat atgattatca
    21121 gttaggagcg acagaagaag cgaatcctga gattagaaaa aagtatgaaa cgttagctga
    21181 aacaattggt tcccaagcat tatggaatca gttgaaagaa aaagacccgg cagctgctga
    21241 gaaaatccac tggaacaatc aacgcaaagt gataagagct ctggaagttt tagagacgac
    21301 aggacatagt atcacggcac ctaaagaaga gccaaagcaa ttgtatgatt acctcatgat
    21361 tggattagat acgaagcgta gtattcttta tgaacgaatc aatcaaagag tcgatttgat
    21421 gctagcagaa ggtttagtag cagaagcgcg taaggtgtat gagcttggag aagtccaggc
    21481 cagtcaaggt attggatata aagaattttt cccttatttt gaagggaaag aaacattgga
    21541 agaagcaaca gaacaagtca aactacattc gagaagatac gcgaaacgtc agttgacgtg
    21601 gtttcgtaac cgtttggatg cacagtggtt tgatcttata agtgagccaa atcaaaaaag
    21661 tcagattgag caaacaatca aaaaatggct ggaggcggaa taaaatggaa gaaaaagtga
    21721 tcatcgtagg ggtagaaacc gaagcaaacc agcgttactt ttcagagtca atggaagagc
    21781 tagttcagct gacgaatacc gcatcaggag aagtcgtatt cacaattaca caaaagagac
    21841 cacaagtgga tcggcaaacg attatcggta aagggaaatt acaagaatta gttcaacaag
    21901 cggatgcgca tgaagcggat ttgatcatct ttaatcatga gctaactccg agacagagcc
    21961 aactgattac cgatgctgtt ggtcttccgg taattgatcg cgtgcagctt atcttagata
    22021 tttttgcgat gcgtgctcgt tcgaaagaag ggaagctgca ggttgaactt gctcagttag
    22081 aatacctgct gcctcgttta gtagggcaag ggaaaacatt atctcgtctt ggcggtggga
    22141 tcggaacaag agggccaggt gaaacaaagt tagaaacaga ccgccgacat atacggaata
    22201 aaatttttgc tgttaagaaa gaattgaaag aagtagaagc tcatcgagag cgcaatcgcc
    22261 aaaaaagaaa aaatagcgag atttttcaga ttggacttat tggctacacg aatgccggga
    22321 agtcaactat cttgaatcta ttgacacaag cggatactta ttcgaaagat caattgtttg
    22381 caacgttaga tccattgact aagagatggc gttttgctga aggatttgag attactgtaa
    22441 cggataccgt cgggttcatc caagacctgc ctacgcaact gatcgatgct ttccattcta
    22501 cattagaaga aagtcaaaat atggatcttt tgctacatgt agtcgatgct agttctccag
    22561 atcgtatttt acaggagcag actgtcttaa aattgatgga tgaactgaat atgaaagaaa
    22621 tgccgatact gactgtttac aataaggcag accagatcga cccagcgatg ttcacgccta
    22681 cgctattccc aaatgtattg atttctactc agacgaaaga aggtaagatg gcgttgatcg
    22741 aagcaatcaa acgtcagctg atggaactaa tgacccctta tacactttct gtgccaagca
    22801 acgaaggaca gaaattaagc gagttgagaa gacaaacaat ggttttggac gaagcgtaca
    22861 tggaagaaac aaacgaatat gaagttaaag ggtttgcaaa aaccaattca aaatggttaa
    22921 gaaaaaacca ataaaatttt atttttttaa
//
LOCUS       DAFCHQ010000044        22712 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000044 DAFCHQ010000000
VERSION     DAFCHQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.044"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(11..1942)
                     /locus_tag="LNC71_001712"
     CDS             complement(11..1942)
                     /locus_tag="LNC71_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6466783.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="LNC71_001713"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="LNC71_001713"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBK6466784.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="LNC71_001714"
     CDS             complement(2871..3623)
                     /locus_tag="LNC71_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6466785.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="LNC71_001715"
     CDS             complement(3782..3991)
                     /locus_tag="LNC71_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466786.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="LNC71_001716"
     CDS             complement(4002..5330)
                     /locus_tag="LNC71_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466787.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="LNC71_001717"
     CDS             5466..5621
                     /locus_tag="LNC71_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466788.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /locus_tag="LNC71_001718"
     CDS             complement(5695..6282)
                     /locus_tag="LNC71_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBK6466789.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="LNC71_001719"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="LNC71_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBK6466790.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /locus_tag="LNC71_001720"
     CDS             complement(7862..9151)
                     /locus_tag="LNC71_001720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBK6466791.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="LNC71_001721"
     CDS             complement(9305..10237)
                     /locus_tag="LNC71_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466792.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="LNC71_001722"
     CDS             complement(10394..11053)
                     /locus_tag="LNC71_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBK6466793.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="LNC71_001723"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="LNC71_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBK6466794.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="LNC71_001724"
     CDS             complement(12094..12384)
                     /locus_tag="LNC71_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466795.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="LNC71_001725"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="LNC71_001725"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBK6466796.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /locus_tag="LNC71_001726"
     CDS             complement(14670..16025)
                     /locus_tag="LNC71_001726"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBK6466797.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="LNC71_001727"
     CDS             complement(16049..17218)
                     /locus_tag="LNC71_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466798.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /locus_tag="LNC71_001728"
     CDS             complement(17215..18102)
                     /locus_tag="LNC71_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBK6466799.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="LNC71_001729"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="LNC71_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBK6466800.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="LNC71_001730"
     CDS             22182..22703
                     /locus_tag="LNC71_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBK6466801.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6325 a         4931 c         4037 g         7419 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata ag
//
LOCUS       DAFCHQ010000045        22174 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000045 DAFCHQ010000000
VERSION     DAFCHQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.045"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            244..2079
                     /gene="tdc"
                     /locus_tag="LNC71_001731"
     CDS             244..2079
                     /gene="tdc"
                     /locus_tag="LNC71_001731"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBK6466802.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2384..3862
                     /locus_tag="LNC71_001732"
     CDS             2384..3862
                     /locus_tag="LNC71_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6466803.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3828..5159
                     /locus_tag="LNC71_001733"
     CDS             3828..5159
                     /locus_tag="LNC71_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6466804.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5273..7825
                     /locus_tag="LNC71_001734"
     CDS             5273..7825
                     /locus_tag="LNC71_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBK6466805.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7832..9271)
                     /locus_tag="LNC71_001735"
     CDS             complement(7832..9271)
                     /locus_tag="LNC71_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6466806.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9617..9979
                     /locus_tag="LNC71_001736"
     CDS             9617..9979
                     /locus_tag="LNC71_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBK6466807.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10166..11023)
                     /locus_tag="LNC71_001737"
     CDS             complement(10166..11023)
                     /locus_tag="LNC71_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6466808.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11143..12054
                     /locus_tag="LNC71_001738"
     CDS             11143..12054
                     /locus_tag="LNC71_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBK6466809.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12061..13707
                     /gene="mdcA"
                     /locus_tag="LNC71_001739"
     CDS             12061..13707
                     /gene="mdcA"
                     /locus_tag="LNC71_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBK6466810.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13809..14108
                     /locus_tag="LNC71_001740"
     CDS             13809..14108
                     /locus_tag="LNC71_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBK6466811.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14098..15774
                     /gene="mdcD"
                     /locus_tag="LNC71_001741"
     CDS             14098..15774
                     /gene="mdcD"
                     /locus_tag="LNC71_001741"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBK6466812.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15778..16371
                     /locus_tag="LNC71_001742"
     CDS             15778..16371
                     /locus_tag="LNC71_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBK6466813.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16395..17471
                     /locus_tag="LNC71_001743"
     CDS             16395..17471
                     /locus_tag="LNC71_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBK6466814.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17496..18365
                     /gene="mdcB"
                     /locus_tag="LNC71_001744"
     CDS             17496..18365
                     /gene="mdcB"
                     /locus_tag="LNC71_001744"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBK6466815.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18476..19240
                     /locus_tag="LNC71_001745"
     CDS             18476..19240
                     /locus_tag="LNC71_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBK6466816.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19729..21822
                     /locus_tag="LNC71_001746"
     CDS             19729..21822
                     /locus_tag="LNC71_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBK6466817.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21847..22149
                     /locus_tag="LNC71_001747"
     CDS             21847..22149
                     /locus_tag="LNC71_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6466818.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7178 a         3787 c         4724 g         6485 t
ORIGIN      
        1 tatttgtttg ttcgcttttt ttaggttatc tattttagaa gactgattta ttgaaaaatt
       61 gttcgctttt ttgcgttgaa ttatcagaaa aatccgactt tatttatttt tgtttgtaaa
      121 aatctacgtg gatttgatgt tataaaaaaa aaattctcat ttttggcgct atagtgaaga
      181 ggtgttacag ataaacacat gtatctgcaa gacattgacc aacaaaatat ggagggaaga
      241 aaaatgaaag atatggatat caaggccgtc tttataggcg acaaagctga aaatggacca
      301 gtttataaaa tgttgttgaa caagatggta gatgaacatc ttggatggag agaaaattat
      361 attccttcag atatgcctgc aattagcgaa ggagataagc tcactccaga ttatctagcc
      421 acccgcgacc atatgataga ggtcttggat gaagtcagtc agcgattacg cgcaggatcg
      481 atcccatggc attcagcagg ccgttactgg ggacaaatga atgctgaaac attgatgcct
      541 gctttattag cttacaacta tgcgatgctg tggaacccaa ataatgttgc attggaatct
      601 tctatggcaa cttcgcaaat ggaagcagaa gtaggacagg attttgcttc attattcaat
      661 atgacagacg gatggggaca cattgcagca gatggttcta ttgctaatct tgaaggatta
      721 tggtacgccc gttgcatcaa atcgattcct ttagctgtca aggaagtgct gcctgaaaaa
      781 gtgaaaaaaa tgtctgagtg ggaacttttg aatttatctg ttgaagaaat tttggaaatg
      841 accgaaagct ttacggacga agaaatggat gaggtcaaag cagcttcttc ccgtagcgga
      901 aaaaatatcc aaagattagg gaaatggctg gtccctcaaa caaaacacta ttcttggatg
      961 aaggcgttag atatttgcgg agtcggctta gatcagatgg ttgctatccc agttcaagag
     1021 gattatcgaa tggatattaa tgctttggaa aaaacaattc gtgaattagc tggacaaaaa
     1081 atcccaattc taggtgtggt tgctgtcgta ggaacgacag aagaaggtca agtagacagt
     1141 gtagataaaa tcgtccaatt aagagaaagg ttaaaagatg aagggatcta tttctattta
     1201 catgttgatg ccgcatatgg cggctatgcg cgttcactgt ttctaaacga agcaggagaa
     1261 tttgtgccgt atgcttcctt agctgaattc tttgaagaac atcatgtttt ccaccactgc
     1321 gtaacgattg acaaagaggt atatgaaggg ttcagagcta tttcagaagc ggattctgtc
     1381 acgatagatc ctcataagat gggctatgtc ccttatgctg ctggaggaat cgtaatcaaa
     1441 cataaaaata tgagaaatat catttcttat ttcgcgccat atgtgtttga aaaatcagta
     1501 aaggctccag atatgttagg agcttatatt ttggaaggat caaaagctgg agcaaccgcc
     1561 gccgcagtct ggacagcaca tcgtgtattg ccattgaatg tgaccggata tggtcaattg
     1621 attggggctt caatcgaggc agctcaaaga ttcagagaat ttctcgatca tctgactttt
     1681 actgttaaag gaaaaacaat cgaagtctat ccattgaatc atcctgattt taatatggtc
     1741 aattgggtat tcaaagaaca aggctgtaca gatttgaacg ctatcaatga attaaatgaa
     1801 aaaatgttcg atcggtcttc ttatatggat ggcgatgttt atggtgaacg gtttattact
     1861 tctcatacta catttacaca agaagattac ggtgattctc cgattcgttt tgttgaaaga
     1921 atgggtctaa cgaaagaaga atggaaaaaa gaacagaaaa tcactctgct acgtgcggct
     1981 atcatgactc cttatttgaa tgatgatcga attttcaatt tttatacaaa aaaaatcgcc
     2041 aaagcaatgg aaaagaaact aaacgaaatc atccaataga actgattttt gtgtgaaaga
     2101 ataacaagag acatatggag aacagacaat tatttgcttc atcaaaacaa ttgtatttcc
     2161 ccctgttttt catatattcc tcgccaagat attctttttt ataaaacgtt ctataagttg
     2221 gatcgttcgt ttcatgtatc aaacccagtt cactaagatc gttcaatgga tgtttcggtg
     2281 atactgggtt ttgtactgaa tcgatgaaac cttgtcattt ctgttcttga acaaaaataa
     2341 aagaaaacaa aaacatatgt cgctattgaa agaaggaata aagatgaaag atactggttc
     2401 atcaaagcaa atcacgacca tgacctttat cggaatgacc tgcgcccttg tagcaagtgt
     2461 acgaaatatt ccagatgtag cagcaacgag ctggacgatg attttttaca tgctggttgc
     2521 agtattattg tacgcttttc caatctcatt gatctctggt gaatttgctg gaatgtttcc
     2581 ccaaaaaggt ggtccagaac tttgggtatc gaatgctcta ggaaaaaaat gggggttcgt
     2641 tgtctcatgg ctgttatggg tccaaatgtt tcctgggatg gtgatggtcg cttcagcact
     2701 cgctccgttg tttggtaata ttatcgataa tgtgccgctt ggtttgaaca gcaaatttac
     2761 attagttgtc attttagtcg tttattggat catcactttt ttgaatttga aatttgatat
     2821 ggcaaaaatc ggcggaaaag tcggtgtttg gttaggattg tacgtgcctt tgacgatgat
     2881 gctcttatta gggtttgctg cgtggatcaa agttgggatc gtaccaaccg gaacattagg
     2941 cagtttttca tgggacaaat taataccaga tactacgact gcatcctcat ttgtttattt
     3001 tgcgccgatc atgtttattt ttactggtat cgaaatgtcc tctgtgtata tcactcgatt
     3061 ggaaaatcca gttaaaacgt atattcgcgg agtatttgcc gcgttaattt ttatattttt
     3121 cgtcaatatt ttgaatgctt tagttgtagc aaatgtcgta ccaaaaggac aaatggaatt
     3181 gaataatatc gcgcaatcta tttcgattta ttgccaaatt cttggtttgc cacatcttat
     3241 tgtgaatctg tttagtttac ttgtcttcat tggtgtagca gtccaattat ctgcttgggc
     3301 ctcaggacca gcaaagacag tgacagaaag tgctagaaaa ggtgcgtatc caccaaaatt
     3361 caatttttgg aagacgaatc aatttgacgt atccaaatcc gttattttga ctcagtctgt
     3421 cattatctca atctttgcac ttttttattt gctgattcct ggcgtcaatc aagcattttt
     3481 gatgctcgta aattcaacta ctgttatcta ctgtatcgtc tatgtcatca tggggatcgc
     3541 agttttgaga ttgcgttata cacatgcaaa gttgaatcga ccatttagaa ttggaaaaaa
     3601 agagaaaaag agcaatcttg gagtttggac tgtcgttatt gttttatttg cggcaatcgg
     3661 attttctgtc ggactgacga tgaaagctgg aacatggatc aatctaattg cagtcacagc
     3721 catcagtgtc attttgtttg tagtaccact ctgtattgaa aaaataaaga aaccttcttg
     3781 ggaacaggaa gtcgccatgc tgcttgaaaa agaaaaaggt gaacttgatg gaaaaaccgg
     3841 caatgaaacg gactctaact aatcgaaata tccagttaat cgcattaggt ggcgcaattg
     3901 gaacaggatt atttttaggt tcagcaaagg caattgcatc agcagggcca gctgttattc
     3961 tcgtttattt gatatgcggt ttggctattt atggtttgat gcgggccatg ggtgatttat
     4021 ttttagcaaa tagtacattt aactctatca gtgatttcgt tgactattat ttaggcaaaa
     4081 agtgggcatt ttttgtcaat tggacttatt ggctttgctg gcttggagct ggacttgcgg
     4141 aattaacagc aataggattg tatatccatt tctggtttgt acatgtacca gctttgataa
     4201 gcggaggtag cgcactaatg atcttaatgg cagtcaacct tgttgcagtc aagtggtttg
     4261 gtgaattgga atcttttttc tcaatcatca agatcttggg gattttcctt tttatcttat
     4321 tgggtatagg attgagtgtc tcagattttc atacccagtc tatcggaatc aaagaaaatt
     4381 ttggacagat cgatgaatta ctaccgtttg gctttgctgg aatggtagtc tcatttccta
     4441 tggttttatt cagttttgct ggaattgaaa tgatcggctt aaccgttggc gagacaagtg
     4501 atccgcaaaa gaatttgaaa aaagcaatca actgtctgcc ttggcgtatt ctctttttct
     4561 atataggaac gattttgtcg attttatttg ttgttccatg gcattgttta gatttaaaag
     4621 aaagtccttt agtgactatg ctgcaagtga tccattgcca aggcggagca gcaattgtca
     4681 atctaatgat tttggttgct tctttgtcct cagcaaatag tgcagtctac agtactagca
     4741 ggattttata tcaaacagca aaagaaaaaa gtttgcccca ttggttttgt gcgctttcta
     4801 agaaaggcgt acctttacac ggtattttaa ttacaggaat gttatttttt gccggtcttt
     4861 gtctacattt ttttgtagct gatagtcgat tgttttttca gcttttggca ggcatgacaa
     4921 cgagttgttt tctatttgtg tggtcttcga tcttgtgtgc acatgttcaa tttattaaag
     4981 aaaagaaacg gaacactaca caaagatata aagatcttgc tttgttggtg ttttttactt
     5041 caattgcttt cagcttgtta ttcgaaagca cgacacgctt tattcctttt ttattggtta
     5101 tctggtttgt attcttaaat tttgtatata aaagaataag caatagaaat atcagttagg
     5161 cccagataca ttttgcaaaa cgaattattt cagacccccc tgaaataatc gatacgcctg
     5221 aaaaaacttc cctgcgtata ataacagtat caattaaaca ggaggggata ggatgaactg
     5281 gtacagattg acggtagaag atgtattgaa aaactatcaa acatcaacag aaggcttgtc
     5341 gcaagaagaa cgacagaaac gcctgactga tcaaggatat aatgaaataa aagcaaggca
     5401 gcagacaaaa cgatggaaaa aaatagcaaa gcattttact gatctgctga tgatcgtgtt
     5461 gatcattgcc tcttttttga aatttgcttc tagtgaatat attgaaggaa gtattatttt
     5521 attcgtagtg atcgtcaatg gactcgttag ttactggcaa gaaagaaaag cggaagaatc
     5581 attgaatggt ttgaagcagc tgatgggaca agaagcgata gtactatcaa acgggatgca
     5641 agaaaaaatc cctgcgaaag aactggtaaa aggagatgtc attgtcttac gtgccggaga
     5701 tgtcgttcca gcagatatcc gattaatcga agcacatgat ttattagtgg aagaatcgat
     5761 tttgacggga gaatcagaag catctgaaaa aagtcatgcc gttttgacag aagaagaatc
     5821 gatcggcgac cagaaaaata tgggtttttc aggaacgctt gtacaatctg gttctgcagt
     5881 cggtgtcgtt gtagaaacag gaaacacgac agaaatcggt aaaatcaatc aagcattgca
     5941 atcggtcgaa caacaaacga cgcctctgat taaaaaaatc aaccgattaa acaaacaaat
     6001 ttttcaaggc attctttgtt tgagcctatt cttagttatt tttacgacat ttcgatatgg
     6061 tatggattgg catatccttc tttcggctac gattgctttg gtggtgtcaa tggtacccga
     6121 aggattgcca gctgttttga ccatgattct atccgttgga gtacatgaaa tggcaaaaga
     6181 aaaggcgatc atcaaaggac ttccttcggt agaaacatta ggttctatga cagtgatctg
     6241 ttcagataag acaggaacat tgacaaaaaa tgaaatgaca gttgtggatg tagctgcaaa
     6301 agaagaagcc tgcgtgttgt ccattatgaa aaactgtcag gaattaaaaa caaaagaaga
     6361 acagaaaaca gaaaatttaa gtggcaatcc gacagaagtt gcgctgattc gacacacaga
     6421 aacagccaat ctacctttga aaaaaataga agcaaagatt ccgttcagtt ctgaatacaa
     6481 atatatggca acgatgcatg cggaggaaaa aggagcggtg atttacgtca aaggtgcacc
     6541 agaagtatta tttgctaaat caactctttc ttctgcagaa caagaagcat ggagtcagac
     6601 agctgcagaa tttgcgagaa aaggtcagcg tgttttagga tttgcttata aaaaagtaga
     6661 ttcgaaacag gaactcacgc atgagacttt aacccaatta acttttgcag ggatcgccgg
     6721 attgatcgat ccgccaaaag aaagtgcagt caaagctgta aaggaatgcc aacaagcagg
     6781 gatctcagtg aaaatgatta caggggatca caaagacaca gcaaaagcga ttgcggaaca
     6841 aattggctta aaacatactg ccaaagtttt agaagggatc gatttggatc ttatgtctga
     6901 tgaagagttg attcagcaag tgccaatagt cgatgtcttt gctcgaacga caccagaaca
     6961 taaactgcga attgttaaag ctttgcagaa aaatggagaa atcgtcggaa tgaccggaga
     7021 cggcgtaaat gatgcacctg ctttgaaaag atccgatgta ggaattgcta tgggaatcaa
     7081 aggtagtgaa gtaagcaaac aggcagctga tatggtttta ggagatgata atttccatac
     7141 gattgcaaaa gccgtaaaag agggccggcg gattttggat aatttgcaaa aaaccatcaa
     7201 tttcttcttg ccaactgcat tagctcaagg attgatcttg atttgggcac ttatgctgaa
     7261 tcgcccgcta cctttgtccc cagtacagat tttatgggtc aatatggtaa cgacaatcac
     7321 tttatcttat gcactagggt tcgagtcgcc agatccagaa atcatgaagc gcccaccaag
     7381 agatccaaag caaggaatcc tatctggtta tcatttgttt cggatcatct atgtctccct
     7441 actgatcatg gttcctgcct atttattggc tatgcagttt gaagggcaaa gcttacagca
     7501 aaccattctt ctgcaaaata tcgtcttagc tcaagctgtt tacatgatca attgcagaga
     7561 gttgtccaag ccgtcattga accaaggtct tttgcaaaac aaagcgcttt ttctttcatt
     7621 aggtatcctt gctttattgc aagggatggt tatttttcta ccagtcggcc agcagctgat
     7681 cggtacagta agtttaacga tgacacaaca gttattgatt ggagcaaatg tgattcttct
     7741 attcttagtt gtagaaatag aaaaagggat catgaacaaa ctgtttcgca aaaaagaaaa
     7801 aacagctaga gaatacagtc gctagccgtt actataaaaa agttttgaaa gtgagttcag
     7861 tcaaacgatt gagctcattt ttcgttggaa tagaagaaat gtatacaata gggatatcta
     7921 gagaatctag agggaaattg gaaatcaaga tatctgtttt ttcaaaagaa atagtggaaa
     7981 gatcggttag ttcgataggt aagaagcaga cacgttttcc taagtaatct tttaattcct
     8041 gctgcaagaa cgccttccat gaaggttctc cttggaaaat aaaatagaca ttgaacaaat
     8101 tcggtttgat agaaaaaatc gtttgttgga tcagtagaga caagagttgg agaaagtaat
     8161 ttggttcatt gatttctatt tcttttttta gatcgttcag taaattctct ttctgttgat
     8221 acaagtgtgc atattgctga tcatatgttt ctaaaaaacg atggtattga cggtccaatg
     8281 cttcaatcaa tatcggcgat tcatgatatt ttaatatgag gagagccaag ttatcagtca
     8341 gagatgtttt ttttagttga tcaagagaca gctctgaagc tagttcctct acaaacgttt
     8401 cgaggacctc taaaaatgga gaatgatgca ggcagcgagc ggtttcttgt ccatagttat
     8461 tgttgtaatt ccaactttcc agaaagcaga aagaagcaaa agaaagctca gtgtctttaa
     8521 gataaatacc ttccgttaaa tacaattttt tcaatgcaga gtcatacagg gaggagagtg
     8581 tgctgttggt atggatagaa gaatctaatc ggcaattcgc ttttatacgg atcgtattga
     8641 taaagaacca acatgttccg aaaatctctt ctgtttcaac cgctagttca gtttgactga
     8701 ggtttcttaa aaattgaaaa taatgggatt catggatcga ataatcctcg aaaaaatagt
     8761 tgttatgagt gaaaggaatc agcaatcgat gataaaaagt acgaatcgca cgctcttttc
     8821 caagaataca agctgtatat ttcgtcaaat cgatctgcaa gtcgtaactt gctaaacgct
     8881 gattgatttt agtcacatgt cttttcaatg tcccataaga aagcccatgg tcggctagaa
     8941 aatcagggac agcaactttt tttgtaaaaa gcagattttt cagcagttcg acactgactg
     9001 attgtttgaa aaaaatctcc caaaggtcat ttgttaataa attttgctgg ttttgcaaac
     9061 tgattcctgc ttgatcgctg atgatgtccc agccttcggg cagctgagag cgaatagatt
     9121 gaaggtcaga aaaaatcgtt cgttcggttg tccctatgat ttcagctaat tcatttacgg
     9181 ttatctttgt atgattcagc aattgttcta ctaaagaaac atgacgtcga atatcttttt
     9241 cggtaatgat ttttttcatt agtgaataca tcaatcagac tcccttctat tctaagaata
     9301 cggagaaaat aaacggtggt caagtttcaa ctgatttttc aatcattgtt tatttttctg
     9361 gagcatttac tgatgataag aatttcatca tgaacttgaa aaatgatacc actaaaatca
     9421 aaaaataggt gggcaaacaa taataaacat tttaatagta gatggtagca agaccaacac
     9481 aggcggattg aacatttaag agttgataaa aaagaaaaat atcgaagtat ttgattagca
     9541 actacttgta ttcatttttc tattcttacg tggtatcttt gaataaagcg aatcaataag
     9601 aaagagatgg aagcagatgc taacgaaaaa agaactaatc gatcacatca atgaaacata
     9661 caatttgtct ccagaatata tttttaaaaa atttcctgat tattgcgtat tcagacatca
     9721 aaaaagcaaa aaatggtttg gggtcctgat gtctgttcca gaagaaaaaa tctatgatga
     9781 tgcaagtgac cataggatca tcaatattct tgatttgaag gtcgatcctg aacttggcgg
     9841 aattttgcga ggaaaagagg ggtattttcc agcctatcac atgaataaag aacattggat
     9901 ctctattgat ctacgtaaaa tagaaaagct agatgaacta gcagatttga ttgatggaag
     9961 ttttaaatca acgcaatgaa tgtctaggac aatagagaag aggttgtgac agaaatgttt
    10021 agcttcaaga aataagaagg aatttacgaa aattgcttct caaatttttg ggaaatttcg
    10081 gcttattttc taagaaatta cttctggaac accgtttgtt cgaaaaaaga gctgcgacaa
    10141 gaattttgtc ccagctcttt tttttttatt ttcctaaata aaattcccgc atcgtttttg
    10201 caatcatttt gatcaaagga tgatcttctg tgaagctaga agataaataa attgttcgtg
    10261 tcaagggcaa atctttgatt tcccgcaaga cgaaaggatc atttttatcg taagaccaag
    10321 aaacttttgg gaaaaagcta acagctaatc cttgcttgac aatagaacgt aatgtcgcag
    10381 gatcatctgt cgtcgaactg atcgtcaatt cgatactttt cgcttcaagt gctgcatcca
    10441 gtgtatgacg gaaggcgttg ctctttgaca gcatcaagat gggggtgcgt tggatatctt
    10501 ctaattgcag atgttctttt ttagcaagag gatgtttctt aggaatggcg acataaacag
    10561 gttctttcat gataggaata ttggtcatgc caggcttcat ctccgaagtg atgtacagta
    10621 caggatcggt aatatcatct ggtaaatgat gttgcatgat cgttaattgg atatcaggat
    10681 gcttttccat aaaaattcgg accaagttcg gaatagaaac agaggctacc tctaaataca
    10741 atatgacgga agtaacgatc ttctcattga ccgaagacaa taatttgatt ccttcttcta
    10801 atgtgtctaa tgtcttgact gcatatggat aaaattcttc tgcataaacg gtcgtctcaa
    10861 tgtttctgcc gctatggtaa aacaaaggga cacctaattc gttttccaaa tgtttgatcg
    10921 cacggctcaa ggcaggttga gaaataaata attctttggc tgcttgggta acattttttg
    10981 tttcgaatac ttttttaaaa tactgcagat gaagcaattt cataattatc catcccatcc
    11041 ataccttttt gttatggttt tttctttttt tggcattttt tattatagca gaaattgact
    11101 aagatagcag tggaaaaagt aagtgctgag gaggcgaggc tcatgagtac aggtttctta
    11161 tttcctggac aaggcagtca gaagttaggg atgttagcgg atatccctga aatgtacatg
    11221 gaacgtttag aaaatgtgac ggggtatcgg ttagttgata aagaagaaaa ctatcaagat
    11281 acggttttta tccagctagc attactgaca aaagcagtat tttatttaga tgaaatggaa
    11341 aaaaacggta ttcagcctga tattgcagca ggacattcga ttggtgcttt ttgtgcagct
    11401 gttgcttgtc gatctttgag atttgagaat gcagcggaac tagtctatca ccgcgcagtc
    11461 ttaatgaaag aagcttatcc aagcggttat gcaatgggcg tagtagtagg tgtgacgaga
    11521 agcgaagctg aggaaattgt agcaaaaaca tttgatcctg accatccagt ttatctgtca
    11581 aatgaaaatt gtccgatgca acatacgtta tctgggagta tcgaaggttt atccgttacg
    11641 ttggcagaag ccaaacggca ccaagcgcaa acagcgaagc tgctgaaagt ccctgtgcca
    11701 tctcactgtc tcttgatgaa tgatacggtc aagaaattca agccgtatat gcagtctgtc
    11761 catctatctg atgctagatg tttgtatata aaaaatacgg atggaagagc cactcaggac
    11821 gcagaagaga ttcgtaaaga tttgttggaa aacctagctt tccctgtgca gtggaaccaa
    11881 atgatggact tggcaaaaga attaggaatg gatctgacga ttgaatttcc ccccggcaat
    11941 actttgacaa agctgattca tacaaaattt ggtgtagata aagggatccg tacgatcaat
    12001 ctagaccaac atggtattga tgacgccacg tttctatatc aaaaatggag atgactaatt
    12061 atggaaaaac caaaccgcaa ctgggcttct aaacgcgaaa aaaaagcgca gcgattggaa
    12121 aaaatcagca acttgctcaa tggcaagtat gtagaaactg aaaaaatcgt agaagtaatg
    12181 gaaaaactga ttgctcccgg cgacaaagtt gtattagaag gcgacaatca aaaacaagca
    12241 agttttcttt cagaaagtct tgaaaaagta gatgcagaaa aagtgaatga tctgcatttg
    12301 atcatgtcta gtatctcacg accagaacat ttgaatattt ttgaaaaagg aatcgcaagc
    12361 aagatcgatt tctcttatgc tggtgcacaa agcttgcgtg tcgcccaaat gattgaagac
    12421 ggcacaatga aattaggtga tatccatact tatttggaac tttatggacg cttattcatc
    12481 gatttgattc caaatgttgt tttagtagct gcagataaag cagataaaga cggcaacttg
    12541 tacacaggtt tcaatacgga agaaacacca acaatcatcg aagctgcagc tttcaaagat
    12601 ggtatcgtga tcgttcaagt taacgaagtt gtcgattctt taccacgtgt agatatccct
    12661 ggtgactggg tagacgttgt cgtgaaagca gataaacctt atcaattaga agctttattc
    12721 acacgagatc ctcaaaacat cacagaattg caaattctaa tggcaatgat ggcaatcaaa
    12781 ggaatctacg cagaacacgg cgtacaatct ttgaatcacg gtattggatt caatacagca
    12841 gctatcgaat tgttactgcc aacttatggt gaatcactag ggttaaaagg gaaaatcgcg
    12901 aagaactggg tattgaaccc acatccaacg atgattccag ccatcgaatc aggttgggtt
    12961 gaaagcatcc atagttttgg tggcgaagtt ggtatggaaa aatatattgc tgctcgccca
    13021 gatgtctttt ttgtcggtaa agacggcacg atgcgcagta accgaacatt atcacaagct
    13081 gcaggacaat atgccatcga tatgtttatc ggttcaacct tgcaattaga ttacgaagga
    13141 aactcttcta ccgtaacaaa aggacgttta tctggttttg gcggtgcgcc taacatggga
    13201 cataatcctg gcggtcgtcg tcattctaca ccagcatggc aatcacttcg cgacaatgat
    13261 gatccattag ggaaaggcca aaaattagtt gttcaaatgg ttgaaacata tggttcaaat
    13321 aaaaaaccag tctttgtcga agaattagat gcactagctg ttcaaaaaca agcaggctta
    13381 gccaatgcac cagtgatgat ttatagcgaa gatactacac atatcgtgac agaagaagga
    13441 attgcttatt tgtacaaagc aaaaacaaaa gaagaacgac aagcagcaat tgcagcaatt
    13501 ggcggtgtta cacctcttgg aatgactagc acgaaagaat cactagatag cttgcgtgca
    13561 gccggcatcg tgaaattacc agaagatcta ggcatcaaac gtagtgaagc gaaacgttca
    13621 ttattagctg cacaaaccat cgatgatttg atcgaatggt cagatggcct gtatgaacca
    13681 ccaatgaaat tcagaacttg gtcataaaat tggcgagaaa aaccttttgt cgccatttac
    13741 ccagatggaa gaagaaagac gaataagcag aacgtgtatg ctctgtttta caaaaagggg
    13801 agagatccat ggaacaatta gcttataaat atccaacgaa acagccgatc aataagaaaa
    13861 tccatgtagg agtggtcggt tcaggagatt tggaaatctt gatgtttcca acagaaaaaa
    13921 cagaatctac tgtaaaaatt tgtacaggaa gcgatggctt cggcgaagta tggaacaatg
    13981 tattaacgag attttttgat cgttatccaa tttcagcaga tatcgtgatc aatgacttcg
    14041 gtgctacacc aggagttgtg tacctacgat tgacgcaagc aatggaggaa ctttcagatg
    14101 aaaaatagtt ttgtagaatt aaacgccaga gaacgagtga acgctctact tgacaatgga
    14161 cgtgaattga ttgatccatt cgatcaaatc atcgcgccaa gtttaccagc acaaggaatc
    14221 gtccctgaaa gtgatgacgg gatcgttgtc agccgtggca cgatcaatgg caaacaagca
    14281 gtagtaatcg ctatggaagg aaaattccaa ggtggcggta tcggtgaagt ttccggtgca
    14341 aaaatcgttg ctgcacttga acatgtgtta gaagaaaata aaaaaggaaa tgagatcttt
    14401 ccagtcattg tactagatac aggtggtgtt cgtttgcaag aagcaaacta cggactgtta
    14461 tctatttcag aaatcggaaa tgtaatcatt gctctaaaac aatatgtacc agtcatcggt
    14521 ttgattccag gacgtgtagg atcatttggc ggtatgtcga ttacttcagc attaatgagt
    14581 tatttaatcg caacaaaaaa agcgagagtc ggtttgaatg gaccagaagt tattgaacaa
    14641 gaagcaggcg ttcgagaatt tgattctagt gacaaagact tgatttggaa tacattagga
    14701 tctcgtcaac gggtagaatc aaaaatcatt gatgaattag tgactgatag tgtagaagca
    14761 atcaaagctg ctgtcatcgc agcaatggac gaacgaaaag acacgcatcg cagtgaaagc
    14821 tcagcatttt atttatcatt acttgattct ttagatcttt caaaaccaat ggccattgac
    14881 gattacaatg aagcaattgc taaccatcaa gcaaaatcaa ttgacttacc agaagtagca
    14941 gaaggaaatg aaccagctgc agcaagtgtt ggttacgatt ggtttgcggc actaactggc
    15001 atgaaaaatc caacttcttc tattagtact gtgtattatg gtaaatcaag taaattcata
    15061 gaagaagcca tcgtgacatc aatcgtacct gatcctaaaa acccgatgta ccgtgtacga
    15121 aacggtgaag ttggtttagt tgaaggtttc cgcatgggac aaattttgga tcacgtttac
    15181 gaagaagata aagacaaaaa agtgaaacgg ccaatcatcg ttgtgatcga tgtaccaagc
    15241 caagcatacg gctataacga agaattgatc ggtatccatg tggctttggc taatagtgct
    15301 gctgcttatg caaaactccg tcaagctgga catccagtta ttggattgat tgcaggaaat
    15361 gctatttcag gtgcattttt agcacatgga ttacaatcaa gccgcttgat tgctttaaac
    15421 aataaagaaa ttaccgtcca agcgatgtct aaagaaagtg ctgcacgtgt gacaaaacgt
    15481 tcggttgctg aaatcgaagc agctgctgaa aaagttccag ctatcgctta cgatatcaat
    15541 aattatacaa aactaggagc gatctatcat ttcctagaag atatcaccga ccaagctgct
    15601 tcagaacaaa atgtcagcaa agtcatcgaa gcaatcaatg aagccattca agatgtccgc
    15661 aatacaaatg acacgatgct gacaaatcga tacacaaacg aaacagccaa aacattcgga
    15721 cgtgtcgaaa caaataaagt ccgtgccaaa atggacgaag aatgggatgc ataaaaaatg
    15781 attcaagcac atgatattgt cctttttgat aaacatgaaa tagaagggtt caccttgcct
    15841 gaatgggtcg ttctaactga aaagatgttc ggaacagttc gtcgtgcgtc gacggatcac
    15901 gcagaaatac taccgattgg tttacgtgga cctgcccgca atcagagatg gtcaacagaa
    15961 atctcaaaac atgccgtcaa acaagtcatt catccgtggg acatcatcga acaagaatcg
    16021 tatagacaag agaaaatcat ggattatcct gtttatcagc aatttcatgc tgctagaaac
    16081 atattatcag gatgtaaatg gggaatcggc ggcagcttag gatttgaatt atcgactggg
    16141 attccggcag taaaagaaac cagtgatttt gatttgctgc tgtacgcaaa ttcaccgatt
    16201 gaattgccaa ttcaggtaat tcaatcgcat cctgcttttt ttgagcaatt cgatacccaa
    16261 gtagtgactc ggcaaggcgg tttttcacta aaagagtatt tgagagagcc agagaaaaaa
    16321 attctattga aaacagtcga aggacctaaa ttaaccaaag aactttggta ataaagaaaa
    16381 agagagagaa agagatgttt gaaaacaaaa aaatcaaaat caacgtaagt gacttccaga
    16441 aagtatcagc tgtaacggct gttatgatgc aaacgatatt gagctttgct ttggcaaaca
    16501 gccacagcgc aacaaataca gcttttcttg gtacctttta cgtcatcgtt aaatacacag
    16561 caccaatgtt tatttttgca attgtttata atatggtaaa aacaagtcag catttgtctt
    16621 attgggaatt tttgaaagaa aagttttttg aactagtcgt tccgtatctt ctatggacga
    16681 cggcttatct ttggctattt ccagcagtcc aacaacaaac tccgtatacg gatactgcaa
    16741 gctttttact gaaatatgtc acaggtgatg gagcgcctca tttatggtat accgttatga
    16801 tgctgcagat ccaattgttc atgccgtttt ttgtttggct cggttacaaa gtatttgcta
    16861 ataaaaaata tgtatggcca gtattgattg gttcaacgat tttatatgtc gcatggtatc
    16921 tgttctatca aacacaagta ttgactgggc cttatcatga cagctggtac ttattagatc
    16981 ggtttgttgc tagtttcatg atttatgggg tagccgcatt tgtttatcac gaaaaagtct
    17041 atcaatattt ggacagagta cgttatcttt tcttacctgt tgccttagtt atcgcgttct
    17101 tttctgtccg ttcattgctc gcacatccag gagacttgag ctttgcgaat gcaccttact
    17161 tgaatacgat ccaaagttta tacagtttag tcatcatctt tgctgtcttc ataggcgctt
    17221 ccaaaatgat tgtaaacgat tcgccaaaat tgccattgtt caaatggttg tctgtttatg
    17281 cttatcgtac gtatttagcc aatgtatttg ttttccaagt actgttatta ttgtttaaag
    17341 atagttggct gcagttacct tcaggagtca tgattcttgt agcttatcta atgacggcaa
    17401 gttgtgcatt tgctttaagc tggttacttc atatcatttg ggtagctatc aaaaaaggtt
    17461 tcagcaagta atagtaggaa gaaggagtga gtgtcatgat aaatgacgaa cgaaaaaaag
    17521 cagtgactca gcaactcgct gcgttggtag aggaagcatt gatcgcagaa gtaacattaa
    17581 gtccaaaacc tggattagtg gatgcactgg atgcgggtgc acattccgat atggactatg
    17641 ctttatttct taagtctgct aagacgctaa ctcctttttt tgaagaaatg gctcaaatag
    17701 catggcatca tgctattgat caggagttaa gagaaagaat tgctgagatt gggcgaaaag
    17761 cggaaaaggc aatgctgatt gctacaaatg gcatcaatac ccataaagga gcgatttggg
    17821 caatggggct attgatcagt gtcattgcac aaaaaatcag tatggaacag cctttttctt
    17881 tatcgaaaat acttgatgat gtcagcaagc ttgctgcttt tcctgataca aaatatcgtc
    17941 ataaaaaaac cactcatggt catagggtga agcaaaaata tggcgtaaat ggtgcatatg
    18001 aagaagcgat attagggtat ccgcatatca ggcttgcaat tcaagcagct aaggagctag
    18061 ataatgattc atatgtacag aaacaaatgc atatgttgtt agttttgatg agttcgctag
    18121 atgacacatg cgtattgcat cgcagtgatc agaaaacgtt agtggaaatg caaaaacttg
    18181 ctcatgctgc tagtcaaagt gctttaccaa atcgtccatt tacagcactg ttgcactttt
    18241 gtcaggaaaa ccaaatctct cctggaggaa gcgcagatct cttgtctgca agcttgttca
    18301 tgctgaagat agaggaatta tggactgccg acacatacat ttgtccgatg ttactcaatg
    18361 actaaataag acaaaattgc taaaaaaaca gtttatgcat ctggttattg actcttcctt
    18421 ttagcaaggc tatcataaaa atagtttttt gtaataaaag ataacgagga agacaatgaa
    18481 aaaaatcaat tatttagcaa tcattttgtt agtgggccta tgtttgtttg ctctgccgac
    18541 ccaaggattc gctgtcgcgg gtggtcatgg tggaggtaca tctagcggtc gaagttcggg
    18601 cagaggttca tttggtggtc gttcatatgg gggcagttat tttggcagtc gttcttacgg
    18661 cagagattct tatgggtatt atggtccagg tacaggtcct tctagttatg agcaaacggg
    18721 atttcttgca ttagctgttt tgtcagtagg aggggtttct gctttttcta tcgtcaagaa
    18781 taaaagacaa aagaaaaggc tgaaagcgga gatattgtcc cgcatgtcag gaacggctaa
    18841 agaaaagaaa gcacagtttc tggaaatcca aaagatattc atgaagatcc aatccgcttg
    18901 ggaagaagaa actcctgaaa aagccagaag ctgttataca gacaagctgt ttgccgaaca
    18961 tcagaaagtt ctgcaaaaaa acaaagcaga cggtgtaaga aatcatacta aaaaaataac
    19021 tattcaggaa gtaggaaatt atcgacagat ccacgaaaat agttttgcca tccgaattga
    19081 tttcagatgc tgtgattatt cagtcgatcg aaaagatggg cgattgatct caggaagtaa
    19141 gacacaacga cagtattttt ctcagatatg gtattttgat ttttctgaga aagaaaaaag
    19201 atggcaagcc gattttattc agccgattat gctggattga caggaaatat ttatgaacgg
    19261 agttgtgaca aaagccttgt cgcagctcct ttttctgaat aaacggtgtg ccagagatag
    19321 cctatcggca ataaggcacg attttgtaaa atatgggaag cgatttttca aaatcgcttc
    19381 ttatcgctca aggctgaaca tctctgtcac aacctctcca ctaaatggtg tttaaaagct
    19441 tgcatctgcg ataataagaa caaccaaacg aaaaatagga agcactattt tagtttagtt
    19501 gttcttatta cttgatgcag gacagctttt tcacaacctc ttttttgtat agatgggcaa
    19561 aatttttgta ataataggcg tatgtctttt tcgttgagga gatgaaaaag catatggctt
    19621 attttttgtt tgctttgctt actttctttc cacttcaagt ggcagaaata tctacttttt
    19681 attatgtaag cggaaccata tacttgaaat cagaaggaga tcatttctat gaaaacaaga
    19741 acaattgaaa tacttcacag aatcatgaca gcccaatatc cagtaaaagc aaaacatcta
    19801 gcaagtgagt tcaatgtcag tcagcggacg atccgtcagg aagttcttga agcgaatatc
    19861 tggttgagca gtaaaaagct tccggagatc cgtacaattc gcaacaaagg ttttttgttg
    19921 aagctggaaa aggaaaagca agctcgatta gaaactgaaa taaaagatgt gaaagacggc
    19981 tttttgaatc ggcgcgaacg tatttttgat ctagttttag ccgttgctta cagccgaatc
    20041 ccgatccatc taaacaaaaa agaagaagaa tttcaaatct ctaaaagtac attagatgaa
    20101 gatatgagac ggttacgagc aacactggtc agttatggga tagagatcgt cagttatgga
    20161 aagcaaggac tggtttataa aggttcagaa cgatctatcc gtacgatgat ctatgatttt
    20221 atcaatcaga atctgggaat gaccgacttc tttatgaatc aagatgcaca attaacaccg
    20281 gctcaaaaaa tctttctcca ttattttcct ttggtggaaa tcaaaaaaat acaggagatc
    20341 tattccaata agttgttgaa acaagaagat gatatttata aaaaacaaat cctgcttttt
    20401 acattgatct ggatccagcg aatcaggaaa catgaattta tctctgccat taattggaaa
    20461 aatacagaaa taaaagacag tagcttttct gcatttattg accacatgat cgaggaattt
    20521 caattggaca atgtcccgca agtagaaaga aattatcttc gtttcactat tgagacgttc
    20581 aatgctagag atatctccaa ttcaatcgag tgggtacaag ctcaattact aacgatccaa
    20641 ttgatccaat ttgtagagaa cgagacacac gtacctttcc atctaaagga agaagcttta
    20701 ttcgaaaatc tctataagca catggctgca ctgattgtaa gaatcaagaa tcatatccaa
    20761 gtcatgaatc cattgaaaga aaacatccga acaaattatt tcccaatcta ccgcgcagtc
    20821 actcattttg ttcctacgat cgaagaagta attggtggca agatactgga ggacgagata
    20881 gcttttcttg tcatccactt ttcgacgatc gccagtgtaa tcaagcgaga cttgaattat
    20941 gtttataaat cagtcgttgt ctgtaaccat gggatggcta ctggaaatct gttagcagag
    21001 aatctgaaag aaaagtttcc tcagatcgag attactgcag ttttaagttc aaaagaaatc
    21061 gaattagtag ataaattgga tgtcaatctg gttttcagca cctttcaatt gaattatcga
    21121 aataagccgc ttttagttgt cgatccgatt ttaactgaga cgaatatctt ggttatagaa
    21181 gactttttga aaagaaatga agaactacag cggttagagc ctaacagtca tgaatcgacg
    21241 gagttattca ctcaaataat agatgtcatg aaagaaagtg gtggccgagt agatcgaatc
    21301 atctatggca agttagaaaa attattcgaa acaaacaatt tagaaatcaa caaaagggag
    21361 atccagccta tgttaaaaga tattttgacg gacagcaata tcatgatcaa agaaaaagcc
    21421 actacttggg aggaatcgat tgaactagct gctagaccat tggttaaaga aaacattgtc
    21481 gaagagcgct atgtcgaagc gatgattcgt gcagtgaatg attacggacc gtatatcgtg
    21541 attggcaaac acatggcact agcccatgcc cgaccagaag atggggtcaa taaattagga
    21601 gtcagcgtag ctacgattga gcaaccaatc gatttcggta atcctgaaat ggatccagta
    21661 aagattattt tttgtttggc agcagtagac tcctactcgc atctgaacat catgaaagaa
    21721 ctaattgaat tgatcaacaa cgaagagaag ttggatcgat tgattgaatg cacaaatatc
    21781 gaagatttca aacaactgct tttttcagta gttgagaaat aataaatgat acgggggaga
    21841 acaaagatga acaaattgaa cattttattc gtatgcggtg caggtttagg aagtagtttt
    21901 gcagcacaga tgtctgctga ggatgttttg aacaaattag gagtagaagc caaactggac
    21961 cattgtgata tttcttccgc agtttccatg aaaccagatg tgatcatcac agcagaaaat
    22021 tttcgttcgc agttcgaaaa gttcgcagtt gatcctaaaa cggcgatgat ctatttgaaa
    22081 aatattgttt caaaaaatga aattgaagaa aaaatcaccc cagttctgca agataaggga
    22141 gttatctaaa tacaaaaaga aaagagggta aaaa
//
LOCUS       DAFCHQ010000046        22172 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000046 DAFCHQ010000000
VERSION     DAFCHQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.046"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..331)
                     /locus_tag="LNC71_001748"
     CDS             complement(<1..331)
                     /locus_tag="LNC71_001748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466819.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(345..602)
                     /locus_tag="LNC71_001749"
     CDS             complement(345..602)
                     /locus_tag="LNC71_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466820.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(797..1339)
                     /locus_tag="LNC71_001750"
     CDS             complement(797..1339)
                     /locus_tag="LNC71_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466821.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1360..2451)
                     /locus_tag="LNC71_001751"
     CDS             complement(1360..2451)
                     /locus_tag="LNC71_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK6466822.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2459..4669)
                     /locus_tag="LNC71_001752"
     CDS             complement(2459..4669)
                     /locus_tag="LNC71_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466823.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4685..7213)
                     /locus_tag="LNC71_001753"
     CDS             complement(4685..7213)
                     /locus_tag="LNC71_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6466824.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7265..7675)
                     /locus_tag="LNC71_001754"
     CDS             complement(7265..7675)
                     /locus_tag="LNC71_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466825.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7688..7906)
                     /locus_tag="LNC71_001755"
     CDS             complement(7688..7906)
                     /locus_tag="LNC71_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466826.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7903..8895)
                     /locus_tag="LNC71_001756"
     CDS             complement(7903..8895)
                     /locus_tag="LNC71_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6466827.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8974..9885)
                     /locus_tag="LNC71_001757"
     CDS             complement(8974..9885)
                     /locus_tag="LNC71_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6466828.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9904..10248)
                     /locus_tag="LNC71_001758"
     CDS             complement(9904..10248)
                     /locus_tag="LNC71_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466829.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10268..10824)
                     /locus_tag="LNC71_001759"
                     /pseudo
     CDS             complement(10268..10824)
                     /locus_tag="LNC71_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10905..11405)
                     /locus_tag="LNC71_001760"
     CDS             complement(10905..11405)
                     /locus_tag="LNC71_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466830.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11406..11951)
                     /locus_tag="LNC71_001761"
     CDS             complement(11406..11951)
                     /locus_tag="LNC71_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466831.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11948..13204)
                     /locus_tag="LNC71_001762"
     CDS             complement(11948..13204)
                     /locus_tag="LNC71_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6466832.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13565..13876)
                     /locus_tag="LNC71_001763"
     CDS             complement(13565..13876)
                     /locus_tag="LNC71_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466833.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13899..15209)
                     /locus_tag="LNC71_001764"
     CDS             complement(13899..15209)
                     /locus_tag="LNC71_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBK6466834.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15823..16275)
                     /locus_tag="LNC71_001765"
     CDS             complement(15823..16275)
                     /locus_tag="LNC71_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466835.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16277..16642)
                     /locus_tag="LNC71_001766"
     CDS             complement(16277..16642)
                     /locus_tag="LNC71_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466836.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16741..21024)
                     /locus_tag="LNC71_001767"
     CDS             complement(16741..21024)
                     /locus_tag="LNC71_001767"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBK6466837.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21171..21410)
                     /locus_tag="LNC71_001768"
     CDS             complement(21171..21410)
                     /locus_tag="LNC71_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466838.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21500..21772)
                     /locus_tag="LNC71_001769"
     CDS             complement(21500..21772)
                     /locus_tag="LNC71_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466839.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(21855..22106)
                     /locus_tag="LNC71_001770"
     CDS             complement(21855..22106)
                     /locus_tag="LNC71_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466840.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6479 a         4804 c         3308 g         7581 t
ORIGIN      
        1 atgaattcat tccccaaagg ccttttaaga ttgatacagc agtccgttca cttcttttag
       61 acttgtcata caagtctttt aaatgatccc atatcagact agtttgtcta tctagcttca
      121 atccattaaa cgctaatgaa cggttcattt tttctattac tttagataaa gctacagaaa
      181 tttttatacc ttcacaacgc gcatcttgga tttcaataat agtatcatca attagttttt
      241 gaatttcttg cctatcaaca tattcaatct tagaaagaga atcttttaaa gctactaatg
      301 attcaacgat ttcttttttc tcttcagaca ttttcttcct ccttctagtg acccgtaaag
      361 ccatgtagag cagagtccat tgaaccagta attacactat ctacaacgcc attccaattt
      421 actctacatt ttccattttt cagatttgtc ggtccatacg gatagccttt ttgacattca
      481 atgttcggat tataccagat aggattatac cattcagccg cagaagcaga ttcagtcgat
      541 acaaacaatc ccacaccaac aaatccaata cacatcacac tatagccaat tagttttttc
      601 atctcgtatc catctcctaa aaaatagaat agttcttata gttattcata agacatattt
      661 tttccgtttt tgttttatcc tttctgtcct ataacaactt ttttcaagta aagaatgaca
      721 tagtttttct taaaaaaaca gtctgttttt ttctatttct tcggtgtata ataaatatga
      781 gtgttcattt tttattttaa tacgcactcc gtacataata aatcttgatc gcatcaatct
      841 tttgagcttc ttgatctact ttcaattgaa gtaatacaaa ttgagtaacc ttattcattg
      901 tttcttctcc aaataacaaa tattgatccg gattctctat atcttgtgtc acctggtaaa
      961 gtttcccagt tgttttcatt tgtacatgcg ggtctacatc aatactattg tctttaatac
     1021 aacgatctgt catatagtcc ctcacagctt gattgcgttt ataaacatct gtgaaattac
     1081 tccattttct agcaaaatct tttaagaaat tttttgtcat ttcatctatt tgcatttgtt
     1141 gcgctgtctc gtcttctaat gaaccaattt gactgttact ttcagtactt tgaagagttc
     1201 cacttgtttc ttttactgct tcgattgttt cttctgtcgt tctcaattgt gttttttgtt
     1261 tttcagaaga aaaccaatga caaccagtaa gacctatcaa acataaccaa ctaaatacta
     1321 aaattatccc ttttttcacg tttctagtca ctgcctttct tatttcacct tacgaaccag
     1381 actacgaatt tttgtttgat tgtacgtgcg ttgataccga gccataatcc gtccttgctc
     1441 ggcattctgt tccacactag agaaatgttg tccgccatat gaaacatctg ttataattcc
     1501 ggtgtgacca tagattccag gagacaatgc accacctgct tgccaattca ccacatctcc
     1561 tgcttgtaaa tcacttggtt ttgggttcat gattacttgc caaccatagg ttgtccagtt
     1621 ataatcttcc cctattaaca tcgcaaacat gtgtccagaa cccattaact gtgggccacc
     1681 taattgagat acataccatg ccgtaccacc ataacattcc ccattattta cggatcgtcc
     1741 aatgatccga tccaaaacct tcgcattgcc tttacttttt cctgcaataa gcgaagtttt
     1801 ttttgttggt aggctattcc aatgcttgat cgcaaaagta tcatattccg ttaagttata
     1861 ggacgttatg atactcattg ctcctcgata atacggactc ccttcggtaa tacctgctgc
     1921 cgcatagcct ccacgaccta gaatcaataa acaacttcca ggatctttat tgcccacaca
     1981 ttgattaaag ttgctttggc tcgccaccat aaagccataa tagtcataac aagcttttaa
     2041 gttcggaaac ttcatataga aaccaccttc agaaccacca attccacgtg cagaaccttg
     2101 tgggtaggga caaccaggaa accaggtaat cccaaaaaag ttcgtatcgt ttcttccaga
     2161 ttgtgactgt ccataagctg attcatagcc taattgagaa agaatcacac tcggtaataa
     2221 ctgatacttt acagctgcac caactgctgc ttgcgcatgt tcatctgtga ttggaccacg
     2281 tcctttaatc tctgaaaaaa tcggtaatcc ttcggtatat tccccattaa aagcgacagc
     2341 tagacctgta gcttctcctc catgctcaga aagttctcct gaaaaaaaga atggcaatag
     2401 aaaaaaggga agaataaaga aaagaagaca acctagacac cctttcttca tttcttcctc
     2461 atcctttctt cgataatggt ttttggactg ttcgtccttt tggtaaagta aaatcagttc
     2521 ctggttttgc gtccaaaatc ggcagtgttt taggatcctt ttctattttt tccgattgct
     2581 ctgttggctt atctaaagaa gaaaatgcag gttgtaacga ttttggtgcg gaaatcgacg
     2641 gtttactttc agacgaacgt tgccccggtt gttgcacaga acgatttcct gatagagtgg
     2701 ctaccgacga attaggaaca gagtattggt tcaataactg ctgcattgct ggataactcc
     2761 catttttcgc ctgatttttc acttggcgaa tctgttgtga aagagaacca gcagctgtta
     2821 attgtggcac gggtactttt ccagaaggca acggaatcga ttgaccaaca aaagcgatac
     2881 ctttatttaa caatggtttt ctttgattat tcccttgttg cttactcaat tgtgccaatg
     2941 attgtacctg ttgttttggt cttactgaat tcatggacga atttggaatc aaaccatttg
     3001 aaagaccaga attccactct gttttcgatt tggtagaaga catttcaggc tctaccccat
     3061 gataacgaac atgacgacca ctataaggca aggcttgatt tgaacgccca cctagttcaa
     3121 agggattaac cacttgcctc ccttttaccg attcttgtgg tgtacgtttt tgttgaaact
     3181 tttgaaatcc atgtatcgct gcagaagcga gtggattcgt tgctcgatac acattccctg
     3241 ctaatcctgc acctttgatt gccaaatttt gtccagcagt tgatagttga ccaggcagtt
     3301 gtttcatttg agacaaagaa tgacttgcta tgtttttcgt gttgtttata gtttgatctg
     3361 cagctcctaa ccctagtttc tttaataaat ctttccgttt tacaatgagt aaaattgata
     3421 ggagtgcttt gacaaacata tttcctaaaa aaccaaaaac agtattcatt ggaattaaat
     3481 cgtctactat gttatagatc aaaaatgcca ccaaaataat cacactgtaa aaagattttt
     3541 gaaacatcaa gcccaaaaag gactttaagg tattcatgag catataatca aatactggaa
     3601 caaaagaagc gagtaatgaa aaaggaatca caagtaataa acacaacacc aacatttgaa
     3661 agataaaacg aatcatgcca atcactacaa gtggtaatcc aatacttaga ttaagcaatg
     3721 gcaccataga accaacgact gctttataaa cagctttagt tggttgaata tagacacgat
     3781 aagccttctt tttccctcta tcttctcctg cagcagtggt cacttttgtc tcaatatcag
     3841 ataattcttc acttgacgct tccttggcta gaaattcttg tggttcaatc tctgcttgat
     3901 acaattcttc taaatttgtt gtcccataat ttaacaatat atacggatca acaacagccg
     3961 ctttataata aagttggcgc actttttgaa tggcttcctt ttgtgtcgtt ggcactaaaa
     4021 caccattgac atcttttgtc gccaattcat ctagactatt tgtagcagaa aaaataaagc
     4081 cttctacctc cacggacaaa tcatttaacc ctttggtaaa agcttctcct ttactaggta
     4141 cgttactacc atagccaaac caaaccattg aaaaaatgac aacgaagaga aatcgaataa
     4201 acaccatttt tgcgtaatgt ttccctctaa atatacgaag agtaaataga tataacacat
     4261 acaagacgac aatccccgca ccaataggat cgtatatttt tgaataaata gtatccgaaa
     4321 aagtgaaaaa ctggtgaatt aactcgttca ttgcagctcc ttcatagagc ttctcaatga
     4381 caaaatcaag gccttgccaa atcaatttac ttaaactaaa gaacatattc gtcatcattt
     4441 ttagaaaatc tgtgccattt gccccaaata acattttgcc taatgaaaat tcactatcag
     4501 ataaataaga agaaaaggta tcaatatgaa atttcccaat ctcatagatc gattgatcca
     4561 cctgcttttc tgccgcatac accaatccac caacaaaaag cagtccgaat ccaatcgtta
     4621 aaaaaaacaa aacgcaccat ttcttttcct tatgctttat ataagccatt gtttcacctc
     4681 atttctattg catgattaac gtatcctgtt cttctacttg agagtgactt gttttttccg
     4741 ttgtttgtaa tgcagcggtc atttcttcaa acaaactgtg tacattgagt tttcctacac
     4801 gtccataggg atctaacatc caacactgtc ctttaatcat gtggctcatt tcagctaagt
     4861 tcgcttcatt tacttccaaa cgtaaatgat tcaaaatctt ttctcgctca ctgtcttcat
     4921 caaaagcaaa gacgacacca aaattccctt tttctttatc tgaacctaag tcaccaataa
     4981 attgtgtatc taacactaat tgattcatct gggaacgccc cacacgtgcc attgctgcaa
     5041 taatactacg tcctgcgttc gaattattaa atacccatgc ttctgtaaaa taaatcgctg
     5101 ttttcttact actatctcgt tttccaaaca tttcacaaaa acgacctaaa ctaatcatca
     5161 aacacaaaga ctttcgatcc gcatctgtat atagttctgg tcggacattg tcatttggta
     5221 atttcaaatc ttggatctct aaaatcgttg ttttggcatt aaagtctaat cccgggtttt
     5281 ctccataaga aaatcctaaa cgtaaaatcg aatcttctac cgtcaagcgt aaaaactcac
     5341 catattctcg aacctctttt tgttccattt gttctaaata gtcgaccaca tgcatcaagc
     5401 caacttgttg tccggcttct ttttgaacta aaacttcttg gattcccttt aataccgccc
     5461 cttttaaaag gtgattggta cctaacggac ttaactcatc aatcatatcc tgggctactt
     5521 gtttcgcatc tactcgggat aaacaaacaa ttggatctaa tacaccataa ttacggggat
     5581 ttgtagcatc taacgtgaca taactcattt tttgcaaatg ttctacaaac aaagggtaat
     5641 gtttctgata atacgggtct tgaatgagtt tcgtaaacca tttgcgcttt tctcctttgg
     5701 gatccacaaa taaactttgt acattcataa acgaactata caaaaagagc aagccaacta
     5761 aaaaggtctt ccctttaccg gtttttccag ttactgctac atgaggcgca tcaaacaacg
     5821 cacctttgat tccttccgca atggctagaa gattcaaaaa gacaaaacga tttgacgcat
     5881 aaattaattc ttctaatggt gttttttctc ctcgagaacc accatcccct aatgtatctt
     5941 gccgaccaat ataccagccg gattccatac ctaattctga tactgctgaa aacaaccatt
     6001 cgccaactgc ttctacagtt gttcggtgct tccaatgtaa ctgccgtccc aatggttgcc
     6061 ctaataaaca tttatagaac aattctcgtt gatcattttt tgcacggtgg atatggataa
     6121 tcgtatgaag ttgttgaata accgttgcac tattttgacg aacttcttca accgtttgac
     6181 cgtatacaat aaaacatgcg atccaatcga cgacaggttg cccattatct actcgttgct
     6241 ttaaatgttt tactaatcct cgaacagtta ataagcgttc actaggttct tcatcatttt
     6301 gaaccaattc cttatgctct gttttgaacc gttccgatag ccaattcagt tttaaaccaa
     6361 tacctccctg tcctttcaag cgtggatagt gaattttcat tcgcaattct gctgggaaag
     6421 aaagatcctg agcaaattga aatagatcac aatttctcat attttcaggt gtttccccaa
     6481 taggcaacaa tgctaaataa ctactcccta actcatcttc taattgaaga aggcctttat
     6541 gttccttcgg tatacgtaca tacgttcctt gattgtcttt cagtacttcc caattatttt
     6601 gttcttctac tttgtgagcc attcctcgaa tatattgcag acgattgaga tacaacagct
     6661 ctttttttac taagggacga atagaaaagc tataaagtaa ttgtgtcact tcttttacat
     6721 aaggatcact ttcagaaaac aaagtttcca aatcatctac ttctaatcca aataagcgtg
     6781 ctaaacttcc tgctaaatct aaccagatta gatttattac atcatgaaaa ctctccaccg
     6841 ttgctttttc tttgactttg attcctaaaa taaatgaagg acgatagact tgaccttctc
     6901 gttccaacaa tgtcactgtt tcttttgcat aatacgcacc aattgcttga ctatcgggag
     6961 aaaatccctt tcctaatgtg tcaaacttcg tccgtaaatt cattgttcgt ggatacatca
     7021 ccaactccac atcttcataa tctgctaaaa gcaacagtaa acgagccatc atttctttta
     7081 tttcctgtgt cttttttggt gtgttttttg aaattgttcg tggaaaaaca tgatagtagg
     7141 cccagataca atcctctcca tttaacaaac aatttttctt gaatgctttt actggttctt
     7201 ttacaagcac caatgaattt cacctcccaa taaaaaagga acaaccagtt gattgtccct
     7261 ttttctaatc ctctaaataa acatacttat tcacaaaaag taattcttcc aatgcattgt
     7321 ttgatacctt gtcccagtta taatacacac aatcaccatt catcatacga atcatataac
     7381 aaaaccaatc ccataaataa aaatgcactt tttttccatc aggttttagt tttgccagtg
     7441 ctaacacaca taaatagggg agtcctatat aaaaaacaac tcctgctggt ggaaaaaaag
     7501 aaatgaccca accaataaat gaacgtaaaa ataaatattc aaagattacg accaacaaaa
     7561 aagttgctgc attgactaat gcaactggaa atggaagacg ccaattgctt gtaatctgtt
     7621 gtacgcgata aggttccgca taaacaatcg aataatcata aggttcccaa tccacttcac
     7681 tatcctccta atccataaat aatttttcaa tgattttacc aaacgcatca aatacacgtg
     7741 ttggatcatt taagaagaaa taaactgcgg cacctgctgc gatagatatt aatgcatttc
     7801 ctacttttga tttcataaaa tttcgcaatc ccagataaac aaccaccatt attaaaaaat
     7861 agccaccttg cgttaatata aaatctacaa ttcccttcag tgtcatttcc gttcttctcc
     7921 tttcctatcc aaccaattat gtgttaattt ttcaacataa tactgatttg tttttttcgt
     7981 aatgacaatc gtcatttgtt ctttttgtaa aagtgacgta tcaccatctc gaaagacgac
     8041 cgttgctttt acaataaaag gttttttcgt ttctttataa cgcaatactt tcatctctac
     8101 ttgatcaact ttagctgttt gattgagtcc ttcaggttct ttcatcatgt atgacatatc
     8161 ttttgcattg tttttcgcat acttatctaa gaaatcttcc acaaatcctt caacatcttt
     8221 cgcttccgac gcatcaaccg tttcgtataa tgtgtcatca taagtaatca ttcgttgttt
     8281 ttctgtattc tttggtagtg ccgtaaaata aggataagca ataatacgac agcccttgtt
     8341 tgaaatagaa aagggaagat tgagtacttg tgtgattgtc acacttgttt ctttcttact
     8401 ctctggattc atcgagcgaa tatcataagt tactttataa ctagcagttg gaatggtttc
     8461 aatttctttt aatccaataa acgaagcagc agttaatttt cgaacacctg aaacacttcc
     8521 tggttcttga ataaagtttt gtgcataaaa ctcttttttt aatgtttctt gacgttgttg
     8581 ttcttgttca tgattatcga atgtattcat atacaaagca acaaaacgat tcataaagga
     8641 ttcaatttca ggtgtgtacg ttaattctga ccgactttgt acttctttct tttgtaattg
     8701 ttggatttca tctttcaaaa acgacacttg aaccttcgtt ttcatggatt gaaaaaatgc
     8761 caagccccct gaaaaacaaa gaaataaaac caaacaccaa atccatttca ctgctttttt
     8821 tgaatggatt ttcttttgct tcggtacttt ctctgtataa actttttctt tccgccgtag
     8881 ttgtcgtttt ttcataagct actccttcta gtatgttttt actagtacgt acgattgaca
     8941 acgataagct tgtttttctg aaaaatagtc cattcaatta ccctcatttt ctattccttt
     9001 tctctttctt ttatatccaa caaatccagc aatacttgtc aacactcctc ctagaacaga
     9061 aagtgcaaca tgtatttctt caccagtctt tggtaagact tttgtttttc catctgctgt
     9121 tttgatttca atattgccac ttggaagttt ctttacagga atcgacaaat cctttaccaa
     9181 attcccttca ctatcttgga ttaatggaat accatctttt acaccaacaa ttgtttgatc
     9241 acctgctact tttaatgggg tggttggtgt tggaatatgg gtcaattcct ctacattact
     9301 agtgacaatc ggtacactca ttccttgaga agtattcgtc gtaatttcac cgcttggggt
     9361 taccgatact gtgacttctt ttgctggaac aataggttta ttttcttttg gaattgcttc
     9421 tgatttttca acaggttgtt tcggttcttc tttttgttct tcactggtag acgacatcgt
     9481 tggctgttcc gtacttgacg aatcagtatt cttttcagaa gaactggttg ttcctgattg
     9541 ttccggagtt gtgactatac ttgaatccgt tgttgttgaa ctatgcacac cttcttcttc
     9601 gatcgaatca gtcggtaaag ttgattcagg aaactctggt tctggtgtca ctggctcttt
     9661 ttcaggtttc tctgactgca attgttcagg ttctgctggt ttttccgttg ttcctccatt
     9721 tagctctgtc tcaacatctg gtaatccttc ccctggcaat gtttctacaa tttctgactt
     9781 aggtgtttct acagtttcat ctgcataaac caatgaactc cctaattgaa acgaaaataa
     9841 tgaaattcct agtgttgtta cgactgctaa tgttgttttt ttcatgatta ttcctccaat
     9901 tttttatgca attcgtgttt tgttcataga cgccgcacta atattatctg aacgataaaa
     9961 gaactcccgt ccgttttctt ctaacccaac atatggatta tttttgaata attgaatcaa
    10021 tagttcactg gtgttgtatt ttggatagtt tgcaaaaaaa taatactgtg cttgtcgccc
    10081 atccgaaaat tcaacaagta attcataacg ataccgcttc aatccttttc gttgttttaa
    10141 aggatctcta taccaacttg tccatcgttc cattcgtgga tagagataga cacgatacac
    10201 atagaacaat gccataccac ataccaccaa aaataataat cgccacataa aaattccccc
    10261 ttttcttcta cctttcttga atcatcgaac attcataccc tctatagaga tttgaaaaat
    10321 ccatcatcaa ttgttcatat aaacgttgtt gcaaatagcg attgtttcga atacataatt
    10381 cttcatagcc atcaatcact tgatcttctt tttcatagca tcttgtagct ctatgacgct
    10441 cagcttgcat gacataacca aactctgcac catatcgttc tactgttaat aactgttgac
    10501 tacgcaataa tgcaatataa caatcacaaa aatgttcaac cgacatttga tttttctcac
    10561 gtttgtgaaa taggtattct ttagaaagca cattattcgt taaacaatct gataaatagc
    10621 gataatctgg cggtcggtta ttttcatctg tgaatttttc ccataataaa ctgattttta
    10681 ttcgttcagc ggctagatag tcttgttcgc gtggataaaa ttcatcaatc gcacaaagtt
    10741 ttttatcacc tgaataaaca aatccatata atgcgaaacg aactcttcct tttcaattgg
    10801 ctctcctaaa aattccagtg acataccatc ttctccttcc taaaaaaaat aaaaaagcag
    10861 aaaccgattt ggcttctact tttctttcct tttatagctc ttttttacgg aagaatccgc
    10921 aaatgacatt ttttcaaaaa atcaatcggc acaagttcac gaaaacttgg agcaattttg
    10981 gatagaatca cttccccatc ttgtaaaata caatcataaa aatcatcagt tacttgatac
    11041 ttacgcttct tacaataatt gataaaatag tagcgatcat tttctatttc cattcgttgt
    11101 tctactaagc gcatacgaac atcatcatag aaataagcca caaatcgccc ttcaagttca
    11161 tgtttttcaa tgggatactc ttctggtaac aatctatttt ttcgtcgttt atacattgct
    11221 ggatacctta gaagaagttc atctaaacgt ctgtcagtat atggtaactg acgaatcaac
    11281 gattctaatc gttctttata cagatagaat aattggcgca agcaatagtg taccgcctct
    11341 ggattttcta atactgttgg atcacgtgac caatacgcac caacataaat cataattttt
    11401 ttcatttaga caatcacacc agcaatacta atgaccgatc cacaataggt agaacaagtg
    11461 gcatagcgac caatttcctc taattctatc tcaatctctt ctaaagaaag atatgtctgt
    11521 tcttctgttg gctggcgact catttcccaa gtgacagtca ccaatgaaat ttcttccata
    11581 agaggttttt cgctgctaag tgctgccaca cagttttcat atatcttcat tttatcgcag
    11641 ccttttttaa ccgctaaaat cgcaggtatt ggcaatgaaa atcgataaaa gcgaatgaat
    11701 tctagccttc ctttccttcg gctaaattcc aactcttcga gttcttgttc gtatccctta
    11761 cgaacttcta ataagcgtgc aatttcttgc cagttctctt tttcctgata acgtccagct
    11821 tttaccgctt gttcaatata aggaactcga aaacgaaaat aagacaaatc aaccatcttt
    11881 tcatttaatg cttcgattgc agcctttaaa tccaacaggc gcagccattc tgttgtcgta
    11941 tatgcattca tgcaattttc cctcctgacg ttcccatcca ttctagatag ttttgttgct
    12001 cgtcatttag ttcggcatta tcaatccctt cttgtacaaa gtctcttcct aaaatacgat
    12061 ctgcttcttg taacaacttt aatgtcggag ctacttgttt caaaaaccag cgttctgaac
    12121 gatctaatga gaatttttct ggctttactg taaaatccaa ttcttccatc gaacgaaata
    12181 attttgccca tcgtttatca acaatccgtg catcactttc cagtctatcc caaacaataa
    12241 gttttgcatt aattaaacca acgccaatct ctcctaaacg tttcggtgaa tcacctaaaa
    12301 taaatgcttc tactgcttgt gttgctttct cattggctaa ccgtaactca tatcgattat
    12361 aaataccata tttcgttaat gcttctaatt ctgaaatttt ttctcggttc gcaatttcaa
    12421 aacgtttctc ataaaaatta aaaaagagag gactctttcg cgatccaaaa tacaaagata
    12481 atccttcact tcgaaaatag ccatcatcta aaaaacaacc gccaatattc tggaatctct
    12541 taaagtccat ttctaataaa cctgcttttt gtttttctag taattcaaag agatcgaaat
    12601 gttcttccgt ttccaatccc caaagttcat ctaacgcaat atcgaatcgg ctacattgca
    12661 cacgtgtata accagcttcc aactttcctt gatttttcca aatggaatat agccatgttc
    12721 gccaatctgt ttgttgttca cgcatgactt cctcatattc ccggcaacct tgtcctgaaa
    12781 attgcacaag tacattttct gttacagttc gttttgaatg ccagaacacc cagatattcc
    12841 cacgaacaga aagacagtca aaaagataca agcccgttgg acgtgtttca aattctgtca
    12901 gatccattcg taatacacgt tgaatcactg ttttgtagtc tgtcccaaaa aaggaaagac
    12961 gcaaataatc aatcattgct cgaagatcta ttccgctttt catatacaat cgaaagcgtt
    13021 ccttgattcg ttgttcaaaa tcctctggga tttttctttt cccttgttcc atctttctaa
    13081 tgtaatcttt tgagcagccc atcaattgcg caaatcttgc ttgtgatagc ggctttttat
    13141 ctccacttat attatgaatt gcacttaact tctttctgta gctttttagc tcaatcggtg
    13201 tcattttctc gctcctttta taaaataaca aaatcgattt tttttaaccc tttagaaaca
    13261 ccgatatatc aactagtccc cctcgttttt tgggaaaagt agtacaactg ctgaccttag
    13321 ataatcactt gatacaaaag ggtttataaa acttttttct atgtgttatg ttatttctgc
    13381 cccccatatt atatatcggg gggctttaca caaagaggcc gtgcgcaagc gcacgacctc
    13441 tttgtgagct gcacacgctg ctagcgagtg cgcattatag gcccatgctt cgcagcctta
    13501 atgcgcaccg ccaggcagag tgcggctacc agactacgtc tagagccctc ccgatagata
    13561 ataactacaa aattaaatga tctaattttg ttatttcact atatttttca ggagaaaaaa
    13621 cgcgataaat tgacaaagtt aaatcctctt ctaaatctac tcgataatga tacaatcgag
    13681 tcaatcgttc tatatctttt ttcgttagca atacttgatt aataactttt cctgtaaaaa
    13741 cacgttcttt gcgcaaaaag cggtaatcag ttacttgttc aatacgattc aaccaatcat
    13801 aaaacgttct ggaagaaaga gaaggattaa tcattcgtat tgctgtagct actctataat
    13861 aacaactctc tgccatttaa cttcacctcc ttagttgatt acaaaatatg cgcatcagaa
    13921 aaacttgttc cttttggatc ttttgctaaa tacatttggc gctctagttg ttcttgtaac
    13981 aactgattat catctcccat cgtttgatct tgactatcct cttttactgt aaaatcatag
    14041 acaatcattt cagtaaattt ctcaaattct ttgataaagt gataatcttt gtaaacaaat
    14101 ggcgcaaaaa actctacagg tacatcaaat cctggaaaag catatccacg accaggttga
    14161 tctttgttgt aaaatggctt atttttaaaa tcttctccaa aagtcattaa ataaccaatg
    14221 ggagacaatc gaccaacaga aatacggaat gttaaattat cccgaacacc acttggaaaa
    14281 tattctgcat ctggtcgttg cattgctaaa ataatgtaca tccctgcttg acgacccttt
    14341 aatactaatg gctgaatcaa tttatcgact ctatttctcg cttccatatc caacgaacca
    14401 taaaaagctg cccattcatc aaaaaagata aaatgcggtg gtaacccata ataagcataa
    14461 tttttccctg gttcatattt aggatctttc atggttttca tataggcata ccgttgattc
    14521 atttctttta atgcatttcg taaacaaata atcatttctt catttatttt gtaaaacaca
    14581 tgtcctttga atacatcgac gtcacctaat gctgctaaat ctgccgcttt tccatcgcaa
    14641 atatctactg tcccaacact taaacaagct tgaattaaag aatacaataa ataggttttc
    14701 ccaccaccga ttgcaccagc aatcaacata tttggtgatt gatcatatac ccaatccaaa
    14761 gttttcatta aatgaatacg tccattttct gctatacatt gatcaatagg aatccgacca
    14821 gaatctgaaa ggaatacata attgattcgg gctttttctc gttcttcacg aattaaatca
    14881 cgcaaaaata cttcagataa caaatgaccc atttttaaaa atcgttcgtg aaaacgccca
    14941 ccatctaaag gaaaagaaac cactaattct tgtttctttt gataaaaata aacttttggt
    15001 aatctcattt tttcccttgt ttgtttctca ctaacttgtt tctttgtata aacaaaccga
    15061 ttgtcaatta acattcgaac aagtaactga cgccaataaa cagaggcaaa atatccttct
    15121 tttagtcgcg accaatgcat caaccctaaa attaacccac taaccagcaa caaatacaac
    15181 acaatactta ttccaggaat aagatacatt tgccaattcg aaaacgtact taaacgtgga
    15241 taaaatactt tccaactaaa ccaacctaac agaatcaaaa atggaaacaa aaagaaacca
    15301 taatagcaaa accataaata acgattgaaa tagcgaattc gccgaccacg atacttgaaa
    15361 aaatgagctt tcaattgtca cattcctcct ttctgttttt acttttcctg tgctaaaaac
    15421 ttttgacaaa aagcaactaa tacaggaata tcacatttag taaaaaaaca taatcgaaaa
    15481 agatttaatc gatttttata agggtgtgca cacatattac atattcgtac tatacgaatc
    15541 caaatcactt cctgatgatc ttgtttccac tggtacggta atgaaaaacg aacaagatca
    15601 cgtaaaaaca tacactgccc aataaccgca caaatatatg catttgtttg tacaaaaatc
    15661 gttggattgt ctccaaaata ctcaatcaat tgttggatat tttgataaca agcacaatct
    15721 ctcaaagtat ttgattggtt ttatctgaat tcaaaatctc cccttccttc tatcttagat
    15781 aaaaagaata ggaagactcc ttcctattct ttcaaaaaaa ccttattttt tctctttttc
    15841 cttagaatca acacttggtt gtttttgatc taaacctttt tcttgtgatt gtttcatatt
    15901 aggattattt gttggagctt ctactttctc taatcctttc gcataaatca ctaaatctga
    15961 atattcacga ttgccatctt gtgctggaat atataccaca tgatattctg gatcaattaa
    16021 tttcacagga tcacgtcgtt taaaaccagt caaatctaca tctgctggaa atttaactcg
    16081 tcgtgcaatc tttggttcat ctggatttcc aaaaactaat tgtgctcgct gtgatcgaat
    16141 ttctcccgta taatcgcctg tttcattatc aaatacacga tcagactctt ctaatgcgcc
    16201 aaagcgtacg gctcctaaac aagtttcatc taatgcttct gtaatgtcat aattatttgg
    16261 aaattttaat cccatcttac aaaacctccc caatttttac accagttaaa aagacaccaa
    16321 catcttgata gccagttgtt cgacgctctg tttgtcccat atcatttgtt tcacgactat
    16381 tcactcgttc tttacgaaaa acaaaataag cttcttcaaa tgaaatacgc acctcttttt
    16441 ctggtaatcg taatctttta tcctcttcag ctgtgagata aacaaaaaaa gcaggctgtt
    16501 cttctcgcga acactctgct tgtaattgaa catatcgccc agacacacca ttaatttcct
    16561 ttggtggatt aggttcccgt gaacccaata aaaatacttg acctaaaaac tctttcttca
    16621 tacgcaatgc tttttctgcc ataaagtttt tcctcctctt aaaaaccaaa ataaattcta
    16681 aatatttcta cattacaatc aaacaagcgg ttaaaaatcc taaccgcttg ttcctcatga
    16741 ctaatctttg gctttacgta ctttataaag tgttatacct aaagcaatga ctaatactac
    16801 taatccaaga accataagta tactacttgt ttgttcacct gtttttggca acattggtac
    16861 aacagtagct ggtttaaccc gttgttctgg tttcactggt tgcggtggtt ttggcgcttc
    16921 cacaatttga accgtttgat ctttgtttgt tagatctaca ttgtgtttcg ccacttcttc
    16981 aaacgtttct ttctcatctt ccgttttcgc ttggagccat tccgcaaaac tcaagccttt
    17041 tcctgcaaga ttgcgaccgt ccactaagaa ttcaaataag aattccatca tttctttatc
    17101 ggccgcaaag actttttccg catgagtgcc ttcaaatggt gtgccgtctg gtgttaagta
    17161 gccttgaaca attaagcgat agatttgacc aatcgcaaga ccgtctactt tgacccattc
    17221 aagcacagtt tgatttggca aggccaagat ctcttttccg ccatttttat tcgtcgcttt
    17281 cgtcgtaatg gttggattac tgattttgat ggtttggtca ttgtcggtaa tatctgcgtg
    17341 agaagccaat aatacgcctt ctctaaacag atcttcaaag accacgactt ctttgcctcg
    17401 caacgcacgt gcgtctagaa taaagtctag attgactgta ccatccgttg tttctgccac
    17461 aaacgttaag gtacttgtga cttcttttcc atctactagc actggttttt ccgttacttt
    17521 gtccattaga acaccgttga ctgtgtatgt ttttccaacg accaaatcgt ggtatttcac
    17581 agcgtctgta atggtgactt tcgttaatgg attaacctct ttcaagccag tgggtttatg
    17641 tgtagccgtt gtacgtacac ttgggttagt tactcgtacc gtttgccctt tgtcgttgat
    17701 gtctttatgg tttgctagtt cttcatcatt tctaaaaaga ctttcaaaca cgacgatttc
    17761 acgaccattt atacctgtca aatcaaaggt aaatggtacg atcaccgttt cattttctgc
    17821 tttggcaata aagccaacac tcgtttctac cgtatttcca tgcacaagca agggttcacc
    17881 ggttgcttta tccattaaga tccctttgac caagtatgct tttcctggaa gaatctcggt
    17941 aatttcaacg gtatcgttta aggtaaacaa tcctgttggg tctgcgactg tcaatccgtc
    18001 ttcaaagaca gccgtggtat gaacggttgg attattcact ttcacacttt gggcttcatc
    18061 gttgatatcc gcatgtgctg caacttcaat gccatcacgg aaaagttttt caaaaacaac
    18121 gatcgtttga cctttcagaa cactagcatt aaccacaaat tcaaggtctt ttgtgccatt
    18181 gactttgtct gcaataaagg ccaaggtact tgtgatttct ttgccatctt gtaaaattgg
    18241 cgcattcgtt gccttgttca tcaatactcc tgaaatggtg tattcttttc cgacaaacaa
    18301 gttggtataa gaaacactgt ctttaatgac gatcgtttct aatggatcaa ccactttttc
    18361 accatcggct ttatttgacg cttgtgtttc tagttttggt ggaatcaagt aattacgtac
    18421 agaaaccact aacgtttccc catcatctgg cgcattttgt ttgaatgcaa ccgtgaatgc
    18481 ttgtttatct gtatttggtt ggaaactttc taatggggca ttttccaaga agtaatagtc
    18541 atcggcaact agacctttca ctaaaatttc cccgttttga tcagtggtat attcaccaat
    18601 tgcttgattt ttaccgttat acaaggtaaa gttcacacct gcgagcggcg ttttcttctt
    18661 gttccattct tcatacttga ataattttac acttttccgt gcttgacggt tcgcaaaatg
    18721 gatcactttg atcggttctg tatcattggt aatcgagaac actaatgggt tttgattcaa
    18781 aacgtaattt acgggggctt tgatttcttc caaacgatag cgatcttttc cataagtaag
    18841 tgtaccgtta gttaggaaaa agcctttttc attggtttta aagatatccg ttgtttctgt
    18901 atcatttgga atctccattg tgacataacg attggctaaa gtatcaaaaa tcttgaattc
    18961 tgtattagca agtggaatca cttttcctgt ttcttgatcc actttcacca ctttaagacg
    19021 ttgttcaatt actttatctt ctaaaacata atggaaagtt tgcccatttt cagaaattgt
    19081 gacttcaagt ggttcaaata gtttataccc ttcttttcct ttggtttcag aaagaatgta
    19141 cgtatcaaag acaacggtat ttttaccaaa agtagcataa ccatcattgt ctgtcacaac
    19201 ggttttcacc actttgccag ttgtttttga cgtcaaagtg atttcaatgc cagctaattt
    19261 tggtttgtta tctggattcg ttgaatcgac taaatcttta ttagcaactt tcactaaatc
    19321 aatggttcct ttgatcactt gatcttttgc ggtcgcattg acagtttttc caggttcaac
    19381 aacaatttca attgcttcat cagaaagcac ataacctttt ggcgctttga tttcttttaa
    19441 aacatatttg ccaggatcaa gatctttaat ttctgcctgt actttgccgt taattttttt
    19501 caaggtaatt tctttaatct ttttcccatc tgcttgatac aaaccataga ccgcattttc
    19561 taaagaagct gcaccttgtg ctttcacacc agtttccgca tcttctttaa acacacgaat
    19621 gtttccttgt tttagaattt gtaaattaat aacagacgca ttaggttctg ttggatgtaa
    19681 cacaccaatt gtttgagctc ctggacgacg ataggctaat ggcgcacctt gaactttaat
    19741 tttattcaat tgaatttttg ctttatccgg tgcctctttt gtgactgtaa tccatacttc
    19801 attgccgtga atagatacat tgacaccttt tggtttttga atgtctttca catatttcaa
    19861 aaaatcattt ttattcttta ctttcaacgt ttcgccaatt tttaccgtat gtgtttgatt
    19921 attaaaagtt gctttttcac caaagttatt aatcaaacgt tgaatttctg cttttcgacg
    19981 atcataatca acacggatat ttttgattgg gatttttgtg cccaaagctt cccaaatcat
    20041 aaattgtgcc gcaatgtatt gttcatcgga tttgtcttta tttgtgacat atcctaaata
    20101 agagatcaag cttgtttttt tccgtacatt tgaatttttc aaatagtttt tcaaagcagt
    20161 agctgtataa acttgacctt ctgcggctga gacaccaggc tcaatacaaa acgccacttg
    20221 tccattaatc tcaaaacgaa ccatataatt taaagtgaag tttagattgt tcggactaga
    20281 agtaacacca atattttcgc cccaatcaat tttccgacca cctaaaacag ctgcattggt
    20341 tgcacggcga acacgaaccg aacgattggt tggtactgtt actaacagtg taggtgtacc
    20401 tgtttttgga atgttctgct cttttttctt ttgattttca atactagccg tagattcttt
    20461 gtttggtttc gttggttgat tttgactagc tgcggttgaa gagattgttt tttcaacgtt
    20521 ttcctcattt tttccttctt tgtcagcagt agccgctggt tgttccactg tttcttctat
    20581 tcgtttcttt tcttccgctt gtttctcaga agactctggc tgcacagaag gcgttgcttc
    20641 ggcagctttt tctccttcta gagtggacgt ttctgaagca ggggttgtaa ttgaggcagt
    20701 ttcttcaact ggttgtgaag taccaggact attccctggg tgttgtgttt ccccaatcgt
    20761 ctgatcgaga gtcgttttgg aattgaccgt tggcgcttct gtcacagggg cctgttcaac
    20821 agtggcttca ccagctggga ccccggcttc tgctggttgg acttctgtca cttcagaagc
    20881 gacttgggca atggcttcca tcgtaggttg tatagccgtc gtttcttctg catgtgcttg
    20941 agcagttccc accaagaaac agaattgaga aaaagccacc aatgcgagtg ctttcaacca
    21001 attcattttc gtttttgcat acacaagaat tcctcctttt cctgagaatt ttatataata
    21061 aaacaaaacg ccgttaccaa aagtgataac gacgtgaact gtctcttcta tttttatctg
    21121 gcgctcttat ctgaacacat ttcatcaatg attttaccct cccactataa ttacagaaca
    21181 aaaaaagaac ctgcttcgtg gaaatcaact gtccatttcc caattggaat accagctttt
    21241 ttggcaactt caccatcacc aaatctcatg ccataccaat tactaccttc atccttaatt
    21301 tctttccaac catatgacat agcttcatct aatgaatcaa aaatcatgac actattgcca
    21361 attgttacag gatctggctt aagatcttct ggaagagaaa tatcaatcat accataatga
    21421 tccgattcct cagtattatt gtcattacta gctactggtt tggttgttct actggcttat
    21481 tttgatacaa caagtactct tattcttctt ttacttctcc gttttcaatt tcccattcaa
    21541 accactccgc aaagtcttgc ttaaaaccat ctactcgata cctattttgt aaataggcat
    21601 cttctgacgt cgtaggatct aaataggtag gtttattctc tggacgagca tagacaccta
    21661 cccatgtttc gtaatctcct tcaaaataac tatctaccaa atagcctttt tttctaagaa
    21721 tctcccgaac ttgattttta attattgata cttgttcaaa accatcactc attctatcac
    21781 ctcaaataac tctagattct cataaatatt cccgacaatt tcaatcctat caatgatctc
    21841 taacaaatcg ctacttatgc ttccccattc gtataagaat ttcccttccg caaaataaat
    21901 tttccataaa cttcttcatg atcgtcccaa ccaatatcac tctcgaaaaa ttccacgttg
    21961 ttcttatctt ttagctcagt aaatgccata agttcaacat gttttccaac ctcgaaacaa
    22021 tcacaccacc catcaaccgt tacacgtgtg ccttcaatac ctaagaaact ttgtcctcgt
    22081 gtattaaaca ctaaaacttt ccgcattttt tgctcattaa ttaatcacgc gcgaaatcta
    22141 ggaatcacaa cttattcgct tcttctttat aa
//
LOCUS       DAFCHQ010000047        22038 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000047 DAFCHQ010000000
VERSION     DAFCHQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.047"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(158..637)
                     /locus_tag="LNC71_001771"
     CDS             complement(158..637)
                     /locus_tag="LNC71_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBK6466841.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(654..1115)
                     /locus_tag="LNC71_001772"
     CDS             complement(654..1115)
                     /locus_tag="LNC71_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6466842.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(1129..2379)
                     /locus_tag="LNC71_001773"
     CDS             complement(1129..2379)
                     /locus_tag="LNC71_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBK6466843.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(2757..4208)
                     /locus_tag="LNC71_001774"
     CDS             complement(2757..4208)
                     /locus_tag="LNC71_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBK6466844.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(4529..5293)
                     /locus_tag="LNC71_001775"
     CDS             complement(4529..5293)
                     /locus_tag="LNC71_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBK6466845.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5321..6019)
                     /locus_tag="LNC71_001776"
     CDS             complement(5321..6019)
                     /locus_tag="LNC71_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBK6466846.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6031..6810)
                     /locus_tag="LNC71_001777"
     CDS             complement(6031..6810)
                     /locus_tag="LNC71_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBK6466847.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6826..7770)
                     /locus_tag="LNC71_001778"
     CDS             complement(6826..7770)
                     /locus_tag="LNC71_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6466848.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7816..8595)
                     /locus_tag="LNC71_001779"
     CDS             complement(7816..8595)
                     /locus_tag="LNC71_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466849.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(8975..11050)
                     /locus_tag="LNC71_001780"
     CDS             complement(8975..11050)
                     /locus_tag="LNC71_001780"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBK6466850.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(11052..11969)
                     /gene="glyQ"
                     /locus_tag="LNC71_001781"
     CDS             complement(11052..11969)
                     /gene="glyQ"
                     /locus_tag="LNC71_001781"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBK6466851.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(12355..13173)
                     /gene="recO"
                     /locus_tag="LNC71_001782"
     CDS             complement(12355..13173)
                     /gene="recO"
                     /locus_tag="LNC71_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBK6466852.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(13316..14215)
                     /gene="era"
                     /locus_tag="LNC71_001783"
     CDS             complement(13316..14215)
                     /gene="era"
                     /locus_tag="LNC71_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBK6466853.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(14230..14625)
                     /locus_tag="LNC71_001784"
     CDS             complement(14230..14625)
                     /locus_tag="LNC71_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBK6466854.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(14609..15085)
                     /gene="ybeY"
                     /locus_tag="LNC71_001785"
     CDS             complement(14609..15085)
                     /gene="ybeY"
                     /locus_tag="LNC71_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBK6466855.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(15114..17306)
                     /locus_tag="LNC71_001786"
     CDS             complement(15114..17306)
                     /locus_tag="LNC71_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBK6466856.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(17330..18301)
                     /locus_tag="LNC71_001787"
     CDS             complement(17330..18301)
                     /locus_tag="LNC71_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBK6466857.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            19006..20385
                     /gene="brnQ"
                     /locus_tag="LNC71_001788"
     CDS             19006..20385
                     /gene="brnQ"
                     /locus_tag="LNC71_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBK6466858.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            20667..22007
                     /locus_tag="LNC71_001789"
     CDS             20667..22007
                     /locus_tag="LNC71_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6466859.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         6175 a         4663 c         3822 g         7378 t
ORIGIN      
        1 atttccaccc ttataactcg gtataaaagt catctcgata ttcttttttt cccaattaaa
       61 attgagcatt tgtgtaatta tacttgtaat tccacctttt accgagctgt cattaccaat
      121 cataagtact ctttgcataa cgctttactc cttatattta tattttttct attaatacct
      181 ctatcgaatt aataatcgtt tccgtgttag atacatactt gttaaacatc cgaactcttg
      241 tttctttaac ggcatcttct aaattttcta attcataaca aggatagata atattcatct
      301 catcaaattg tctaattaac tgcagttggt gatcatcgac atgctcacca tacttagcca
      361 atcgaggaac agcaatgacc cttttacctt tttttacggc cccaattata gcacctgtac
      421 ctccatgagt aatcaccatt tctgcttttt gcatagtttc agtaaactca tccctattta
      481 gaaatgcttt ataggaataa tttagtggtt tataatcact ataaccactc tgtgcaaatg
      541 tactttcgat tattactcct ttttcaatta aatcatcaat agcttttaac aatctatcaa
      601 attgaaattt ttgtgaacct aatgttataa atatcatttt tttctcctat tatttaatat
      661 atcccaccca aataaatagc attaggataa aattgtagca tagtttccca ctgaacataa
      721 aattgatctg caaaatgata catcaatttt cctgtctgag tagggctggt tacttttgca
      781 aacgattcaa tataaatcag tttttttccc atcatttttg ttattaagca gatagggatc
      841 atagctaaaa cacctgtaca aatcacgaga tccggttttt cctttatata tataataatt
      901 gatttgaata aattaataag tatagtacac aaaaaaaact tttcttttct atttacttgt
      961 ctcatgtagt acgttttttc gccttttgac tcagctgaat attctgtctg ttctgttata
     1021 acaaaactct catacttctc catcagtggt tttaacatca ttaactgttc ataatgcccc
     1081 ccagaggaag ccgcaaaaca tatctttttg ttcatattaa cctctctatc agtcacgctg
     1141 aaaaatatct ctagtgtaaa ctttttcttt aatatcatcc aagcaggtat catatcgatt
     1201 ggcaataata gaatcacaca tcgccttaaa ctgctctaaa tcgttgataa ccttacttcc
     1261 gaaaaaagtc tctccatctt tcagtgcagg ctcataaatc accacagtcg cacctttagc
     1321 ttttacgcgc ttcataactc cctggattga actctgacga aaattatcac tgttgctctt
     1381 catggtcagc cggtatatac cgattaaaac tggattttcc ctgttgacat catactgatt
     1441 atattcagcg tagttgtaat agcccgccat ctgaagaaca cgatctgcga tgaaatcctt
     1501 acgggtacga ttagattcaa cgatagcctc aatcaaattt ccaggaacat ctgcatagtt
     1561 tgccaacaac tgcttggtat cttttggcaa gcaataacct ccatatccaa aggatggatt
     1621 gttgtagtga gttccaatac gaggatcgag acaaacacca ttgattatct gctgagtatt
     1681 cagccccttc atttcagcat aagtgtctaa ttcattgaaa tagctgactc gcagtgctaa
     1741 ataggtattt gcaaacagct ttactgcttc ggcttcagtg aatcccataa aaagagtatc
     1801 cacgttctcc ttaattgcac ttttctgcag aagtgttgca aaaacatgag cagcatttaa
     1861 caagcgctca tcagtcatat cagtaccaac aataatacga gatggataca gattatcata
     1921 taaagccttg ctctcgcgca gaaactctgg actaaaaata atgttcttac tccccgttgt
     1981 ctcacggact gattcagtgt agccaacagg aattgtagac ttgatgacca tgatggtatc
     2041 aggattcacg tccatcacaa cctcaataac tgactcaaca gcagaagtat cgaaatgctg
     2101 agtacgactg tcatagttag tcggtgtagc aataacaacg aaatctgcgg atttataagc
     2161 gctccttgca tccaaagtag cagttaagtc aagttctttt tctgtaagat acttttcaat
     2221 atattcatcc tgaattggag acttccgttt attaatcagt tcaatctttt cagggataat
     2281 gtctaccgcg gttacctggt gatgctgcgc caatagtgtg gcgatactca aaccaacata
     2341 accggtgccg gctactgcga tgttgtactt tttcttcaca atatctcctc tttcctctct
     2401 taagtatgga gaatgttact cctacggcta ttcatcatgt caaaataaca cagcttagaa
     2461 tgttgataaa ataattttcc agaatgtgat aaaatctctt tcatttataa accacacatc
     2521 taattttatc tattctccac aagacatgta gagtattctt taataacaag atacgacctt
     2581 actaaatact tcaaaaaatt atcatattta attcataaat ataaatcttc attttaaatg
     2641 tatgcccaaa aatttatctg taatgcgtca tcaaaatcta gattccaaat ctttatcaat
     2701 ttttatccac tcattctacc actttagtaa tattttctat ttataacact ttcgtcctac
     2761 attgatcccc ccctctgaag tacagttttt actgtcttaa ataaaatttt aaaatctaac
     2821 cccatactcc aattttttat atattccgtg tcaagttttg ctacttcttc aaaatcagta
     2881 atatcacttc taccacttac ctgccacatt ccagtaattc ctggcttaat agcaattctt
     2941 gcacgatgat gataatcata ttcttcatac tctgatttaa ggcatggtcg aaatcctata
     3001 aggctcatct ttcctgttag aacgttgaaa aattgaggga attcatctaa agacgtggat
     3061 ctaataaatt gaccgattcc tgtctttttt gttccatctg gaagtatttt atttccgata
     3121 attcttggat caaactccat cttaaacata agattattgc tcatcttatt ttctttcatt
     3181 aattctactt ttctttcttc tgcatccata tacatacttc taaacttata catcttgaac
     3241 ggtttaccat tctgacctat acgttcttgt gcaaagaaaa ttgggcctgg tgaattaata
     3301 taaataagtg gagcaataaa gataaaaata attcccgttg caatacatcc aacaatacct
     3361 gcaagaatat caattgttcg ttttataaat gcctgttgag ttgtcataac attgatactt
     3421 gttgtaagta ctgtatattc tcctatacga ccaatagatt gttttcccaa agcactatct
     3481 gatacttttg caagattcaa atgtacagta aggcccattt cagagcatcg ttcaataaat
     3541 tctttcatac acgaagtact tttattcaaa ataataaaga cttcatctac ccatccctga
     3601 cataagtaat ccgctgcatt tttggaatca gaaacaactg tcactccctc aacatcttca
     3661 ccaatcatgc ttttatctat gataatgaca ccatttattt tatacatttt atagttatgc
     3721 tctttcacat tacgaataac tacttcagca atactactag atgtaactat aataagtgat
     3781 ttgttcccct catttttcat tttatgtttc aagtaatgtt tccataagag gcgagttata
     3841 tatgtaatta caacgtataa acctcccatc aagaaaagaa cgactctaga ataatcattt
     3901 ccaccctgaa cactaatgag atacaaactt cctaacagca ccaacataat cgcttgcttt
     3961 acagaagaag caaattctat atagtaccct ctttttaata cacctctata tgcttcaaac
     4021 atgaaaataa ctaaaagatc tgccagttca atgaagactg ccatgttttg atacattgga
     4081 atcacataag gattccaatc tacctggcgt aagacatacg atagataaaa agcaagctgg
     4141 atacacaata aatctaaaat aataaaatca atatgtttaa cccatcccct tgaacttttc
     4201 ttgtacattc ctttttactc ccctcttttt aataaaataa attgtattac ttgttttctc
     4261 tcctaaatat ctttctgaag gattattgac tcgacaaatt ggcttgtgga tattcttata
     4321 tatagaacat ccgtaagcca acttaaaact ccttaacaaa atttttttac tcatattgta
     4381 gatcaaaaca agtatccaag aaatcatatt ttatttcttt tttctgctta ctcaacaaca
     4441 gctaaaagaa aaatcatcat aaacaaattc aactaagcca acgatcccct tgtgaaatca
     4501 agttttttct tgatttacga ataaagagct aaaataaacc aaacttcttc ttttttacct
     4561 cttcatagac tggataactg atttcatccc cgttgatcag atcctttgcg acttgtttca
     4621 tctgtactac tttttcctta ccaaagtcct ttgcaatctg ctcatagcac tcttttaagt
     4681 aaaaggtgcg cttagagacc ccatgggcat cagaagcagc catctgcacg agattatgtt
     4741 tgaccatcgt tttggctgtt ttctggatgt ctttgccaaa taccccaaca taacttggcg
     4801 cagtgagttg ggccaaacat cccatatcta ggaacggaag caaatgattt gggtcttcgc
     4861 gaaaatgcac gttgcgttct ggatggacga ttactggtgt ctttcctagt tctaacaact
     4921 caaaaaaaag ccgttctgca tataacggca cttccagtgt cggaaattcg atcagaagat
     4981 atgtatcatc taaatctgta aataatattt catctcggtg gatgtcttcg atgagcgttc
     5041 cggtaatacg tacttcttgt ccttccaata acgtcaaagg caactgtctt ttctctaatt
     5101 cggcttgtaa cgaagcgact agagaaatga cttgtgattt ttcattgctg tattttccat
     5161 tattgtggtg tggtgtgcat aaaatatgcg tgatgccttg ctgaatcgct ttttcagcca
     5221 ttgcaatact tgcttctaag ttttctgccc cgtcatcgat tcctggtagg atatggcaat
     5281 gtagatcgat catttctctt gtctcctctc ttcttattcc ttaatttccg tagtaataac
     5341 cagcatcttt gctatgttct gctccgttgt acacaacacc taagactcgt gcttgtacca
     5401 tctccagcag ttcttttgct ttagtcaagg attctttacg tgacatattt tcacgtacga
     5461 ccaataaagt gccatccgtt tttaaagaca tgatctgtgc atctgttacg gctactactg
     5521 gtggcatatc gaatatcacc acgtcaaata actgtcttgc ttcggatagg atctgatcca
     5581 ttcttggcga gcttaacaat tctgatgggt tgggtggctt aggtccacta ggcaaaatcg
     5641 acaagttctc gactggcgtt cgttggatca catctgctac acttcctgat gaactaagtg
     5701 ctgtacttaa tccactggca ttgttcagct ggaatgtctt atagacgacg ggtttacgta
     5761 aatctgcatc gactagtaga actcgttgac ctgatttagc aaaaacgact gcaatgtttg
     5821 ctgcagtggt ggatttccct tctccaggac ctgatgaagt cacgacaatc gttttgattt
     5881 gctgaccagc tgcggaggca aactggatat tcgtccggat cgtacgatat tgttcggcga
     5941 ttggtgaaga tgggtttgtt aatgtgatca aactgactgc atgagtttgt gctgcttttt
     6001 gtttctgtgt tcgtgccatt tttcttcctc ctagactctt gagcggcttc tgcgtgatgg
     6061 ggctgtttta tcgtttgtag cttttggtcg tgcaggagct gttccttttt tacggatacc
     6121 agcgttcaat tctttcgctg acatttctgg aactgttcct aagattggga atcctaatgt
     6181 atctgtcaca tatttttcat ctttcactgt ccgatcgagt aattccaata agaatgccag
     6241 acctacacct accatcattc ctaacattaa gccagtcatt aaattcaatt tattatttgg
     6301 cgaaacaggt gtcgtattgg caactgcacc tgaaatgatc gagattttat caacattcat
     6361 cacatctttt gcgttctctt ggaaaacttg tgaagttgtg ttagcgatct gttgagcagc
     6421 cactgcattc gtacttattg cctgaatcga gaacatttgt gaattttctg cctgattaac
     6481 tgagatggca tctttgattt gttcagcagt cattttcaca ttgtcttctg tttctaaacg
     6541 atctttgact tcattgagca ctaaatcact gacgatcata tctttatacg tattgatcat
     6601 ttgcaggttc gtattgacat cattgacatt tgcagtgtct gattgtggca gtgtcacgat
     6661 caattgtgct tgtgaactgt attttggtgt caagatgaaa aatgttacga ttcctgagat
     6721 ggccaagcca gcaaacattg agatgatgat tattaccaag tgtttgcgta atgtttcaaa
     6781 aatttctttc aagctgattg tctcttccat tttttctcca ctttcctatt tattctctag
     6841 attcatttga tcttttaatt cttttttcac acgatcaagt tcttgatcac tgactttttg
     6901 ataggagata ttatcttgca tgaagccttc tccttgcagc tgatcttgct taatcgtacc
     6961 aaatgcctcc cgataatcta aagcaatttt tttcatttca tcaaagttca tgtctgtttt
     7021 catattcgac tctagagcat tcaaaacgga ttgatagttg ctgacaccgt ttagactcaa
     7081 tacttttttc gctacgcctt ctacgatctt tctttggcgc ccttgtctac cgtaatcacc
     7141 atttgggtct tcataacgca tacgagaata agataatgcc tgatccccgt tcaatgtaat
     7201 tttaccgatt ggaaagtcat agccatcttg tgagaaagtg aggtcattat tgacttcgat
     7261 accaccaact gcatcaacga gttcctttag tcctttcata ttgatagaga catagtgatc
     7321 gattgggatg tctacatatt tttgtacggt atccatcgcc atggcagctc cgccgaatgc
     7381 gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg atgtcgacat acgtatctcg
     7441 aggaatgctg acgatcgttg tctgtttgtc ttcaggactg actgtggcaa ccatcatcgt
     7501 atctgatctt ccttgatcga cccgacctaa atctccagta tcaatcccca tcaatagaac
     7561 agagaaagat tcttgtttgt ccagatcaac tggtttcgtt cgttctcctt gggttcgttc
     7621 cactgattcg taagttttct caatagaatg tgagagatcc agatataatt ttgctcctac
     7681 tcctacaatt gctaatacta atacgataag aactgctaaa ataatcaaaa atattttttt
     7741 ccctcttgat tttcttcttc gtcttgccat agtttcctcc gtttgctaac cttgcttgaa
     7801 tcaacgtttt aactgtcaat agcttatatt gctatagccc atttgctgag caacagtttg
     7861 tgcttttgtt gcgtatgcgg ctcttatttt ttcattccgg atctgctcta tttgactgag
     7921 cgccgtcaaa tattgatcgt aagtagcggt agctgttact tgaccgtctt tcagcatcgt
     7981 atcgaatagc tgatctagtt gcgtgacttg tgcaatttgg gtatcagcga atcctagata
     8041 ttgcataacg actgttttcc aagcactatc cttaaaaaag ctcatatttt cacgtacatg
     8101 ggcgacatct gcacttgcca acttttcttt gatgatgaca tcatccacag cttgctgcca
     8161 gtttgatacg tttttagtaa ataatttatt gacagccgtc tggatcttca gttttgattc
     8221 tgcatcctct aactgcgtta agactgcttt cttttcttct tggatcgcag cagcttcttt
     8281 aggtaattct gattccttga tgtcataatc atctgccgaa agtttgatag aagatagttt
     8341 atgtgacaaa tttgaaactg tttcttctgt tatttcagga tttaggaaaa ttttttcttt
     8401 tgtactgtac agcttatcta cttcttttga taaagaggtc acggtgtctt tttgattggt
     8461 gatggcagat tctgcgtaat tgatccgttg atgatattgc cgctgattat tgagatggta
     8521 tccaaacaag caaatcacaa tagcgcctgt gcctaccgcc acagtagcca ataacttgac
     8581 gaacggcttt tccatagatt cactcctaaa tattgatgat agataagtaa agatgtgatt
     8641 cattcacatc agaatgagcg ctttttattt taaaagcata attaaactaa tatattcata
     8701 gaaatattta taaacaggtt ttccctatat aatcatacca catacagttt ttttgactag
     8761 ggaaattcct aatttaaaag taaataaaac agtaacaaga ttataattaa tcgttttcat
     8821 ttttatttcc tcacttttca tgagcgtttt gaaaattcga acaaagaata aaataacaaa
     8881 aattattaaa atagaattaa taacagctat ttataacaaa aaaaacagag acacagcacg
     8941 tttcgtgcat gctctctgtt tttcaaacaa aagactattt tgtgatgatt tgatccaagc
     9001 ttgctaatga caaagccatg gcagctattt gacttagttg tgttagtcgg ttcttacgta
     9061 ctgctggttc atcagccatg accatcgttt gatcaaaata attttcgatc agtggacgaa
     9121 gagcgacaag ctggtcgtag ttttcgtgga tcgtttggtc agcaaaactt gctttcattg
     9181 cttctactgc tttgtgtaat tctttctctg cgtcgttttc gaacagctca gggtctactt
     9241 ctgattgttt ttcaagctgc gcttttttcg ctaagttcac gacacgtgtc aaagcttcca
     9301 ttgaaggttt gaagtcttca tctgcaagat gttgtttgaa caattgtgct gctgcaaata
     9361 cttttgtcag atctttttgt tccgcattca aaacagcatc gatgacatca tgacgaatat
     9421 tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcact tgttgttgcc
     9481 cttctgataa accaatgccg aaacgatccg tatctttact gataacatcg ctaatcgttt
     9541 cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgatccga acgacaccgt
     9601 atgcttggcg acgtaaggca taaggatcgt ttgacccgct tggaatcatc ccaactgcaa
     9661 agaaagaaaa gatgctatct aatttatctg caatcgctaa aacagaacct actgtagact
     9721 gaggtagctc tccttcactg gatataggca tgtaatgctc acgaatcgct gtcgctacag
     9781 caggtttctc gcctttcaga agagcatatt tttcgcccat gatcccttgt agttcaggaa
     9841 attcccctac catgttggtt actagatcaa atttatagat ttcagcagct cgttgcaaat
     9901 ccatcaattc ttcctcagat agtcctactt gttcgccaat gatacgggca atgacttgta
     9961 cccgttgcat tttctcatag atactaccga ttttttcatg gaaagtaaca gtttttagtt
    10021 tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat tccgcatctt
    10081 ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac atcttccaat tttacccgat
    10141 caccattacg agcagaaata aaatgaggaa gcagcatacc ttgatcattc cgtacctcga
    10201 aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca gaaagatatt
    10261 tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg acttcttcta
    10321 gtaaatcttc gtctagatca ataacccagt gatttttttc tgctaattcg tttgcttgtg
    10381 taacgatcat ttcttgtctt ttttgcggag acgcaatgac gaattgttca ttcaattttt
    10441 cttcatattc ttttgcatgc gcaaatgtca catcttcgcc taagaaacga tgacctcgag
    10501 aggctctgcc tgttttgaca tccaagattt cgaatgggat tacttcgtca tctaacaatg
    10561 ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa tgcatcgtta
    10621 cagggaaagt caaacttttg atcacccctg ctaagcctgt caatacttct tttgcttcct
    10681 taccatgagt gtatttcgtt acatagacat attccacacc ttttaattct ctaaaagtga
    10741 tagcgtctgt cgtcagacct tgtccgcgga caaatccttc cgctgctttc gtccagtttc
    10801 catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca ctgtccgctt
    10861 gtcgttctgg aatatttgtt actttgattg ctaaacggcg cggtgtggaa aatgtttcga
    10921 tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct tctaattgtt
    10981 tcatgcttgg tgtgacaaca tgggcaggca tttcttccag cccgatttca agtaataagt
    11041 cttttgccat cttattcagc ctccttcacg gtttctggat ttgctgtgtt tttattcaac
    11101 aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc acgagccatg
    11161 ttgcggatgc gagaaagata accggcacgc tctgttacgg atactgcacc gcgggcgtct
    11221 aataagttga atgtatggct acatttcaag atataatcat acgccggatg aacaagattt
    11281 tcatcgatac agcgtttagc ttctttttca aaacgatcaa agttgcttag tagcaagtct
    11341 tggtcgctta cttcaaaaga atatttggaa tgttcatatt ctggttgttt gaagatctcg
    11401 ccatatttca cgccatcagc ccattctaaa tcatagacac tttcaacttc ttggatataa
    11461 gaagcaagac gttctaatcc ataagtgatc tcagaagtta ctggtttaca aggcagtcca
    11521 ccaacttgct ggaagtacgt gaattgtgtg atttccattc cgtcaagcca tacttcccaa
    11581 cccacaccgg cacagcccat ggatgggttc tcccagttgt cttcaacaaa acggatgtcg
    11641 tgttctaatg gatcgattcc taaaaggcgc aagctttcta aataaagctc ttgaatattc
    11701 tcaggtgaag gtttcatcac cacttggaat tgatgatgct gatacaaacg gtttgggttt
    11761 tcaccataac gtccatcagc tggtcggcga gatggctcta cgtaagcagc attccatggt
    11821 tctggtccga ttgcccgtaa aaaggtatat gggctcatcg tacctgctcc tttttctgtg
    11881 tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa tgttaaaatc
    11941 atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt taaagcgttg
    12001 ctgtcaaata agaatacagg aaagaaaacg acaaaaaatc cctatgtctg attacagaca
    12061 tagggacgaa taattcgcgg ttccacccta cttccgaata aagattcggc agcttcattt
    12121 atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggctcccact ctcccgaact
    12181 cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt tgcagttttc
    12241 ttgtttctat aatagatcct atcactgaac gatctattta atctatggtt aataatagac
    12301 ggaactccct gtgtttgtca aatggttttg attttttgtt gtatgtttta tttactattc
    12361 ttctttttca ggtgtctctt tttctttttc agacatgtct ttctctgacg tctttaatac
    12421 gtgtgcccaa gacttcattt gattgatgaa ctttttgctt ttcaaatgga taccgacata
    12481 ttcatcatac agctggtcga tgagttcacg gattgcttgt ttggtttctt ctttcagatg
    12541 gattccacta attttatcat aagaaatagc tgaaaacatc cgaacgaaat aaacagctcg
    12601 aggatctgca tgataacggt gatcgtccat cgaccaatgt ctttcgcata tcacaccacc
    12661 atatttggaa gaatagtcaa attttccttt tgtttccccg catacgacgc agtgtgtcca
    12721 gattggtgca attccgaagc gttgcagtat ttggacttca aaaatattcg tgatgatttc
    12781 tgcatcgtct ccttggtcca tccgttgcag tgcttgttgc gtaaactgga acaaatgcgg
    12841 gtcgtactga tgatcttcga ttgccgcatc tactagattc aataaatatg tgccatagcc
    12901 gctcaaaaag atgtcttctt ggatgtgtcg aaacgcatgg acatctttga cactgcttaa
    12961 aaatgacaaa ccttcacttc gaaaattccc gatataaact gcttcagtaa aagggagcaa
    13021 agccggagct aagggctgat tttgccgatg gatctctttg atataaaaca tcattttgcc
    13081 agaagattct gtaaagattt tgactaattt atctttttct ttgtaattac ggctagataa
    13141 aatcaagcct tttgattctg caagctggct catattctcc ctcctaaaaa ataaatctgg
    13201 aaaagcagat tctgccgatc cagatcattt atgttgcgat cttccgcagc ttttctcaac
    13261 acgcggattt ttattcagtt tatttctata cctaatttac aagaagctca gaatatcagt
    13321 attcgtcttt tcggtagccg aagtcttgca gatagacttt tttgtctcgc cagtcttttt
    13381 gaactttgac ccaaagttct agaaagactt tgtctcctaa aagattttcg atatcttttc
    13441 ttgctttcgt tcctacttgc ttcagcattt ttccgccttt gccgataatg atcccttttt
    13501 ggctatctct ttcgacaata atcgtagcct ggatatggat tttatcattc tcgtttcgct
    13561 tcatcgaatc aacaacgact gcgacagaat gaggcacttc gtctcgtgtt aaaaacaaga
    13621 ctttctcacg gatcaattca gaaacgatga agtattcagg atgatcagtc acttgatcat
    13681 ctggaaaata ttgtgggccc tctggcattt cactgactaa tacatccatc aaacgttcga
    13741 cgttgtttcc ttctgtagca gagattggaa tcacttgtgc aaaaggcatc tgcgttgaat
    13801 aatcttcgat gattcccaac aactcgtctg gatggatttt gtcgatttta ttgataatca
    13861 gataaacagg agaatgcacg ttttttagac gttcaataat aaaatcgtct ccccgaccgc
    13921 gtttttgatc agcactaatc ataaataaga ccgcgtccac ttctttcaac gcactgtaag
    13981 ctgcttctac cataaaatct cctaaacgat gctttggttt atggattcct ggtgtatcaa
    14041 taaaaatcaa ctgagcttct ggtgtagtgt atactccttg gattttattt ctagttgtct
    14101 gtgccttgtc actcatgatg gcaatttttt gacccacgat tcgattcaat aatgtggatt
    14161 tccctacatt tgggcggccg acgattgcga caaaccctga tttatgttct ggcatatatg
    14221 ttctcctttt taattaaaca acaaatgata tattttaggg acaaataaaa tcaacccaac
    14281 gatgacggta aaacaagcgg tcactaaaac tgctccagct gccatatctt tgattttttt
    14341 cccaatcgga tgaaaatgaa aatccgtaaa catgtccacg acattttcaa aaacagtatt
    14401 gatgatctct actatccata cgagaaaaac agctaaaagc agccataacc attctatccg
    14461 atcaagttgg aaaatcacgc ccataaacaa agcgaggatt ccgaacgcaa catgtttcct
    14521 catgttgcgt tcttcctcaa aaacagtttt gactccttgg acggcaaatt ccacggacat
    14581 caggaaatgt ttgtttttct ctacgttttt atcttttaag tccataggca tctaagatct
    14641 ctttctgtaa accaaacatt tctttctcat cctctggtgt catatggtca tacccgttga
    14701 tatgcaagaa tccatgaacc gctaaaaagc caagttcccg atcatacgaa tggccgtaag
    14761 cttctgcttg ctctgcagca cgttcagtag agatcatgat gtctcccagt acacgaggca
    14821 atggattctc tccatcttca aaaatcactg ggatttcgtc ttcgccctct tcttccaagg
    14881 caaaactgat gacgtctgtc ggtgcatctt ttccacggta atcacgattg attacttgga
    14941 ttgctgcatt gtccatgaaa gtaacagaca tttctgtttc ttctggcaac tctaaatatg
    15001 atgctgcaaa ttgcagcaag tcgtcgattt ctttcatttt ctcttctgag acttggtttg
    15061 tttcatccat gaaggtaata tccattatac atcgcttctt tctattcttc ttgctattct
    15121 tcttgttctt cttttatctt ctcaacttcc gatttcattt taggatattt gatccgagaa
    15181 tggaaggtac tgcttaaacc gctgatcaat gatttttcta ctttacggat ttcttttaac
    15241 gtcaatccac tgtcgtccaa ttgtccatcg gaaatacgct cttcaataag attatggaca
    15301 aaactttcga ttttttcgct cgttggatgg tccattgccc gtactgccgc ttcacaactg
    15361 tcagctatat tgacgattcc tgcttcacgg ctttgaggtt ttggtccagg ataacggaat
    15421 tgctcttccg taacttccgg gttgcgttcc tttgccttaa cgtagaaata acgcatcagc
    15481 gtggttccat gatgctgtct acaaatatcg atcaccattt gcggcatatt ataatcttcc
    15541 aatattttgg caccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat
    15601 aagaaattgt gcggattttc cgctcctgaa ggcaagttct caacgaaaaa gctggcgtgt
    15661 ttgatcttgc cgatatcatg ataatagcaa gctactcgag ttagtaaaga acgtccaccg
    15721 atttctgcta cagcattagc acttagattg gctaccatca tgctatggtg atacgtacct
    15781 ggtgcttctt ccagcaactg tttcaataaa ggatgattgg gattgctgag ttcatttaat
    15841 acgatcacac tatcatcatt gaccatcaat tcaatatagg ggtgcagacc tatcgtcagc
    15901 aagaatgaaa acagtgcacc tgtgaatccg cagactagcg ttaaccaagt cgttgcatcc
    15961 ccaaagctca ttccttggta gataatcaaa atgacatcca tcatcacagg aaaaataatg
    16021 acccacatag cagcggataa cccttgttct gagatccgtt tgcgtctgac gatcgttgcc
    16081 atgagaccag aaaatacata ggacatcaaa ataactgtca gaaagttcgt gccaatcgaa
    16141 tcataaaaaa tgaaaagtgc ggatactgct tgaaaaatac cagccatgat ccctgctctt
    16201 cgattcaaga aaaagcttaa aacaagtgga gcaaaagccg cggggtaaaa cagcggaata
    16261 taagcagctt gttctgtttg gaagagttgg aaaaatttca ttagtacgac gcttactgac
    16321 attgccgtca cgtagaaaag tacgtatttt gttctttctc ctgcattttg gaattgaagt
    16381 gaattataaa tcagtacacc gatttgaagc aaaacagcta aaaccatggc aatgagtggg
    16441 aaaatagaag tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca
    16501 tcaatttgat ttccttcgcg aacaataatt tctccttgga agatcatcac tggttgtaca
    16561 gcttctcgtg cagattgttt taattcttct gtctttttct cattcaatac atcattaact
    16621 acgatgcctt gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa
    16681 tattgaatct gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt
    16741 ttactcatct gttcgtcaat gagcttcaaa ctttcatccc taactgtgtg aatttccgtt
    16801 tcagtcattt caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg
    16861 ttttctgcat tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta
    16921 ggtttcggga tattttcatc ttccttcgcc ttatctacct tgccattata agatttgttg
    16981 atggtatcat tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca
    17041 tctgccagat ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt
    17101 ctttgttcgg tttcttccgt gttctcaatc gttttattcg cacggatact ctcttctgct
    17161 acttggcctt ctctgaaatc aatggccttt tgtttgacac tactaaatgt aacgccgcag
    17221 acaatgataa aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt
    17281 agtttctgca gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat
    17341 gaagatccgc cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt
    17401 cacttgcttc aaaattaaca aaatcgattt ttttgatttg ctttaatgtt cgttctgcat
    17461 gaacaagacc acttgtcgtc ccacgaggca agtctatctg acttgtatcc ccattgacaa
    17521 tcatttttga ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt
    17581 tttgtgcttc gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta
    17641 aaggtgcgat ttcgatcacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa
    17701 tttggtataa agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc
    17761 cgggtagaaa gcctaggttt tcccctgcct ccacagcggg ccgcgtcaaa atgatttttt
    17821 gcacttcgcc tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg
    17881 ttccggctgg ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct
    17941 tttgaccgct gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct
    18001 catacatctc aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacaa
    18061 catctggcgt acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg
    18121 aggcagccaa ggaaatctgg gattgctcac caatgatctg aatgatttct ccacgtgtat
    18181 ggatggttgt ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa
    18241 gcatgctgac atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca
    18301 aaaatagaca tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa
    18361 ctatatagca aataagaaaa ggttgtaaaa gagtcgctta accatacgta ccaagaaacg
    18421 ttaaaaaata gccgtttcca ttcgctaaag gtctcgtttt gataaaaaga agctgagaat
    18481 gtaagttata tgaaagtgga gaacaaaata tttcgtttca ttcaacaagt attctctagg
    18541 tatttttcct gtttcaaaaa aagaaaacaa aatagttaaa atattcttgc atttcaaaaa
    18601 aaacaggtgt attattacgt caacaaataa acatacagca aagcgtcgag gaaaacagta
    18661 ggctattcaa cgatttattc ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat
    18721 agacgaacat catttccgag tcaatgtcga aaacgtagat cattgtggct gcctccatta
    18781 tcaggctagc ggataacttt atcaaaaagc gtccgtgaat gaagaagctt ttttaagcga
    18841 aaaaaggtgg taccgttcta gaatctagag cccttttccc aagtggaaaa gggctttttt
    18901 ttattttaag gtggtggttt tcatggaatc tgatattagt ggcattacta attgctgcaa
    18961 cgtttatatc gtcaatcaaa atgataatta ttggaggaaa aatttatgga agaaacaaaa
    19021 acttattcat ggaaacaact attgatggtc agttcactta tcttcgggat gttcttcggg
    19081 tcaggtaatc taatctttcc tgtccactta ggtcaaatgg ctggcgcaaa ctgggtcagt
    19141 gctggcgtag gatttgctat ctcaggtgct cttttcccat tactagctat cttagctgta
    19201 gtagtgacaa aaagtgatgg tctgtatgac ttagctaaac ctgtcggaaa atggtatgct
    19261 gccttgttct taatccttgt tcatttgacg atcggaccat ttttcggtac accacggaca
    19321 gctgctactg cttttgaaat ggctgctaaa ccatttttgc ctgaaaaata taattcatta
    19381 ggtatgtttt tattctctgc tcttttcttt ttacttgctt atttccttac tgtccatcca
    19441 acacggttgg tcaaatacgt cggaaagtat ctaaactcct tgtttcttgc attattagca
    19501 atcatttttg tcgtttcttt attccatcca atgggaaatc taaatcaatc tgctgcagat
    19561 acttatcaaa caaacgcttt aacaaatggt gtcctagaag gttataacac ggttgatgca
    19621 gtagctttgc ttgccttaag tgtcactttt gtccatgcag tccgtgacct tggtttcaaa
    19681 gataaaaaag tcacagaatt aacggcaaaa gctggtagcc tgagcgttgc gctagaagtc
    19741 atgatctatt ttggacttgt tcttctcggt gcgatgagtt tgaataagat gtctttatca
    19801 gaaaatggcg gaactgcact ttctcagatc actaattttt atttaggtaa cttcggcgct
    19861 agcttcttag gagtgatggt cacattaggt gtcttcacta ccgctatggg actcgttgtt
    19921 tcttttgcgc aagatttcca taaactgttc cctaaagtca gttatatgac atggctgcgg
    19981 ttaacgactt tcgtctcttt tgtcgttgca aatgctggtt tagacaatat catccaatgg
    20041 tctttaccag tattgatgct tttgtatcca ttgtcgcttg cgttgatctt gctttcctta
    20101 acagcaaaat ttttccaaaa gacaccattt gtctatcaag taacgatgtt gtttgctgct
    20161 gttcctgccg tattggatat gctggcaaat tcgcctgctt tagtgagtca acaaagagtt
    20221 gtagcaagca tgctcgaatt ttatcatcat cacgttcctt ttgctgcatt aggacttggc
    20281 tggatggtcc caacgttact tggttacgct ggcagcctac tgttttatta cgcctatcgc
    20341 ctatcagggt ataaacaaga agcaaatgaa ctaccagaag aataaccaaa tcaaacacaa
    20401 tttaacataa gaaatattaa acaggaaaaa accagctaga tgaaagaatc ctattctttc
    20461 gtctagctat tttttagttc tcttttttac gcgcacaaaa aaactgcgga caatgattgt
    20521 ccgcagttta aatgggctct ataatatcta gtgttggtga cgccttctgt tgccaatact
    20581 ttttcttttt tctaataaac gcaaacagac accttgaaat cctttagaat aaagtcgacg
    20641 aaaaccattc aaaggagtgt ttcaagatgt cctattcaga gtttaccaaa gagttactag
    20701 atattctaga cctaaatctt atctttcatg aagatgcctt tcgcaaagag cgaatcaatg
    20761 acgaaacatg ttttgttttt gatggcacat tgacctatca accagaggaa tgcttccatt
    20821 gtcactatca aaataagcaa acgatcatta aatggggctg gaaaaaagta tccatcttat
    20881 tgaatgatgt cagtaactac aagaccattc tgcggattaa caaacagcgc ttcaaatgta
    20941 aacactgtgg aaagactttt ttagcagaag attcagttag tgatcgtcgc tgctccattg
    21001 ctcgcagagt caaacaagcc attcttgagc tattaagcga acctctttct atgtctttga
    21061 tcgcacgtat gaagcacatt tctccgacca ctgtcattcg aatacttcgc agccttcggc
    21121 ctaaaaccgt ttctttgaac cagccgttac cagaagtggt gtgtttcgat gaattcaaat
    21181 cggtgaaaaa cgtctcggga gccatgagct ttgtcatgat ggacggcaag acccatcaac
    21241 tgattgatat tgtggaaaat cggcaactaa atcctttacg cgactatttc ttgcgctacc
    21301 ctagaaaagt ccgtgaacaa gtacgtctag tggtttcaga tttctataca ccctatcgca
    21361 cattggtgaa agagtgcttt ccgaatgccc gaatcgtcgc cgatcgcttt catattagcc
    21421 aacacatcgg tcgggccttt accaaccacc gaattcaagt aatgaagacc tttaaaaaaa
    21481 gggatcgtcg gtcgaagcac ttaaagaaat actggagact acttcaaaag aatgcgtggg
    21541 aattgaaagg tcagcatcgc tactggcgtc cgtcatttcg agatcatctg acagaagcag
    21601 agatagtcga tcggctatta tcttatgatg actccttaaa gcgaggctac gaagtctacc
    21661 aggattttct ttccgctatc agaagacaag atgttcctga attcgtcgcg ctcctaaaag
    21721 aagactacaa ggagttgcca gagcactacc aaccagtgtt cacgaccttc aagaaatatc
    21781 gaacagaaat caaacgagcg ttgcgagttc cctattccaa cggccctata gaatgtttga
    21841 acaaccatat caaagtacta aaacgaattg cctatggctt tagaaacttt caaaactacc
    21901 aagaacgaat ctttttatat cgaggaaaat attttaagaa aactaagaac actacccaac
    21961 tgactaaagc tagaacaaca acacggctag acaagatagc tgtctagccg tgtgagtatc
    22021 tcgattattc tattgaat
//
LOCUS       DAFCHQ010000048        21956 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000048 DAFCHQ010000000
VERSION     DAFCHQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.048"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(4..534)
                     /locus_tag="LNC71_001790"
     CDS             complement(4..534)
                     /locus_tag="LNC71_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBK6466860.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(633..983)
                     /locus_tag="LNC71_001791"
     CDS             complement(633..983)
                     /locus_tag="LNC71_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBK6466861.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1140..2669)
                     /gene="ahpF"
                     /locus_tag="LNC71_001792"
     CDS             complement(1140..2669)
                     /gene="ahpF"
                     /locus_tag="LNC71_001792"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBK6466862.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2679..3242)
                     /gene="ahpC"
                     /locus_tag="LNC71_001793"
     CDS             complement(2679..3242)
                     /gene="ahpC"
                     /locus_tag="LNC71_001793"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBK6466863.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3386..4048)
                     /locus_tag="LNC71_001794"
     CDS             complement(3386..4048)
                     /locus_tag="LNC71_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6466864.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4232..5878
                     /locus_tag="LNC71_001795"
     CDS             4232..5878
                     /locus_tag="LNC71_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK6466865.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5869..6951
                     /gene="uxuA"
                     /locus_tag="LNC71_001796"
     CDS             5869..6951
                     /gene="uxuA"
                     /locus_tag="LNC71_001796"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK6466866.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7059..8612)
                     /locus_tag="LNC71_001797"
     CDS             complement(7059..8612)
                     /locus_tag="LNC71_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6466867.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            8760..10049
                     /locus_tag="LNC71_001798"
     CDS             8760..10049
                     /locus_tag="LNC71_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6466868.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEAAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(10175..11254)
                     /locus_tag="LNC71_001799"
     CDS             complement(10175..11254)
                     /locus_tag="LNC71_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBK6466869.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(11363..11803)
                     /locus_tag="LNC71_001800"
     CDS             complement(11363..11803)
                     /locus_tag="LNC71_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6466870.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(11860..12270)
                     /locus_tag="LNC71_001801"
     CDS             complement(11860..12270)
                     /locus_tag="LNC71_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK6466871.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(12451..12885)
                     /locus_tag="LNC71_001802"
     CDS             complement(12451..12885)
                     /locus_tag="LNC71_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6466872.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(12985..13833)
                     /locus_tag="LNC71_001803"
     CDS             complement(12985..13833)
                     /locus_tag="LNC71_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6466873.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            14222..15988
                     /locus_tag="LNC71_001804"
     CDS             14222..15988
                     /locus_tag="LNC71_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBK6466874.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            16090..17349
                     /locus_tag="LNC71_001805"
     CDS             16090..17349
                     /locus_tag="LNC71_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6466875.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            17496..18797
                     /locus_tag="LNC71_001806"
     CDS             17496..18797
                     /locus_tag="LNC71_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6466876.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            18800..19651
                     /locus_tag="LNC71_001807"
     CDS             18800..19651
                     /locus_tag="LNC71_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6466877.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            19664..20479
                     /locus_tag="LNC71_001808"
     CDS             19664..20479
                     /locus_tag="LNC71_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBK6466878.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            20630..21796
                     /locus_tag="LNC71_001809"
     CDS             20630..21796
                     /locus_tag="LNC71_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6466879.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         7327 a         3974 c         4046 g         6609 t
ORIGIN      
        1 tgattaacgt atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat
       61 gaaaatcaaa ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa
      121 caataagacg aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc
      181 ttttgtattc atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag
      241 aagaatcggc aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat
      301 caggaaaaaa agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg
      361 gtaagcctca aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc
      421 gataaaatga aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac
      481 agcagctctt aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga
      541 aaacctcctt tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa
      601 atgagcgaag gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt
      661 ttgccttttg ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta
      721 tcctcgatgt cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga
      781 ttggggacag gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat
      841 tctagttctg tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt
      901 atacgatctt ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc
      961 gtaaaaatac gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac
     1021 ttatagtata taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt
     1081 gacaaaattc ttgtcaccat ccttttttcc gctctctgac tttttacaat cttcatagct
     1141 caagtgcgca tcagataatc gaatgctcct aaagctgcag tagctcctga acccattgaa
     1201 acaatgattt gtttgtatgg actatctgta caatcaccag ctgcgaaaac gccggggata
     1261 ttcgttgcgc catttttctc ggtcactatt tcatttcttg gattcatttc gatcgttcca
     1321 tctagccaat ccgtattagg gagtaagccg actagaatga atactcctga aacagctagt
     1381 gtatgcatct ccatcgtacg gcgatcgata taggagagag attctacctg attcgtaccg
     1441 ctgatttctt tcgtcgctgc atgtttgata acagtgacat tcgcaagaga gactagttta
     1501 tcctgtaaga tttgatctgc ttttaattct ggtaagaatt ctagaacata tacatgtttt
     1561 gctaacccag ccaaatcgat tgctgcttca acaccagaat taccaccacc gataacagca
     1621 atctcttttc ctttaaataa agggccgtca cagtgtgggc aataagcaat tcctttattt
     1681 ttaaactctt tttcgcctgg tacattgata gaacgccagt gcgcacctgt cgcgatgatg
     1741 gcggtttttg ctttaagtga tataccgtta gctaagccaa tattaataaa atgttgctct
     1801 ttatttaagg aaacggcctg ttgattcatc ataatgtcga tagggtaact acgcacatga
     1861 ctttctactt gctgcatcaa ttttggacct tctgtgtaag aggtaccaat catattttca
     1921 atccctagtg tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt
     1981 cctttacgtg ctgcatagat agctgcacta ctagctgctg gtccaccgcc aattactaat
     2041 acatcataag gttctttttc aaacaactct tcttcaatca aagggccagt gattttttca
     2101 acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa aaacacagtt
     2161 ggcactgcca tgattttttt atcatcgatt tctttttgga acatcccacc ttcaatcatc
     2221 gtatgagtaa tatttggatt taatacagct aatatattca atgcctgtac aacgtcgggg
     2281 caattatgac aagtcaagct aacataactg tcaaaatgca atgtttgtgt tatttttttg
     2341 attttaagac tcaaagattc actgattttc ggtgcccgtc cactaatttg taacatagcc
     2401 agtaaaaaag aggaaaattc atgaccgagg gggataccag caaaaacgat tccactttct
     2461 ttgccaactg gattgataga aaaactaggt gtgcgcatcg aatctctttt ttcccaagag
     2521 attttaggag acatttctgc tatttccttc gtaaattcag ctagtttttg actattttca
     2581 ctattatcca aactaacaga aaaaacaata ggagattcaa tcagtgctaa atattgttcg
     2641 agattttgtt tagtttcttt gtctaacata tccaccacct aaatctttcc aacaagatca
     2701 aaacttggtt tcaatgtttc gccactttct ttccattttg ccggacaaac ttcacctggg
     2761 tgggtacgta catattgagc agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg
     2821 attccatctg cattgatttc catagcttga acgattccgt ctggatcaat aataaatgtt
     2881 cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga aatctgatga
     2941 gacggatcac cgatcatcac gtattcgatt ttaccgatag catctgatgt atcatgccat
     3001 gctttgtgag taaagtgggt atcggtagag caagaaaaga cttcaacacc taaagatttc
     3061 aatgtactgt attgttcttg taagtcttct aattcagtag gacaaacaaa agtaaaatca
     3121 gctggataga agcaaagaat actccagttc cccataagat ctttttcact gacttctaaa
     3181 aattctcctt gatgataagc atgtgcatta aattcttcga tttttttccc aatcaatgac
     3241 atagtaaaac ctccaataat aattatttat ttataattat tgtaatttag ttggagttgc
     3301 aaagtcaatg gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa
     3361 aaatagaaga ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa
     3421 cagtttcctg ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt
     3481 ctggtttttt atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc
     3541 atggaagccc ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt
     3601 catctaacca gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata
     3661 aattatctgc acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt
     3721 ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca
     3781 tgatttgctc ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa
     3841 ttttggaaac atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt
     3901 gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt
     3961 cagatatttt ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat
     4021 aagcttgtga ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt
     4081 agtataacag aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta
     4141 taaatagtaa aattttggtt gacatagcga tttttattgt ataaaattca cttgaataca
     4201 aaaactaata tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt
     4261 tatccctgaa ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag
     4321 agaaaatgga caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg
     4381 atttcatgca gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat
     4441 tgtttgtgaa acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt
     4501 gattcttgaa gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac
     4561 agctgacagt ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca
     4621 ctttgaaaat cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt
     4681 gaaagataca aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa
     4741 gaagccatca catacaatga gtattcttgc tgcgctactt tataaacgtt ttttatctgg
     4801 agcttggccg attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg
     4861 tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt
     4921 tatccaatac ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat
     4981 caccccgaat ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat
     5041 gtcgattttc caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat
     5101 ccattattta atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg
     5161 ggtattcttg actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg
     5221 ccttaatcct ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat
     5281 agctgaggag atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga
     5341 aggcctccca gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt
     5401 aattactgtg cgcttttcta atccaaatat tcctgatatg cctcagcgaa tcgccacgga
     5461 tacctctcaa aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga
     5521 agttaaagac ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata
     5581 ccttattggc attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac
     5641 agactttcag cataagcttt ccgatattca actgggtacg caagatacat cacatattca
     5701 tgagaaaatg gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc
     5761 agagcttggc agaaaaatag aagaaatgtt catcaagatg gtagcaggac cgaatgccgt
     5821 acgaaaaaca ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact
     5881 tttagatggt acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct
     5941 gcggtcaaag ggattgtagg cacattgatg gatataccag taggagaggt atggcccagg
     6001 gagaggatca aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt
     6061 gaaagcgtca atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc
     6121 gaaaactata agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat
     6181 aattttatgc ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc
     6241 tcatccacgt tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg
     6301 cgcatcgaac attccagcaa tgaatttgaa ttgccgggat gggaacctga gcgcttagct
     6361 catatcaaat ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat
     6421 tttgcctatt ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct
     6481 gtacatccag atgatccgcc atactcgatt tttggattac ctcgaatcgt aaagaatcga
     6541 gaagatttag attggttgtg ccgcatagta gacagccctt ataacggaat cactctttgt
     6601 acagggagta ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgt
     6661 caaagagatc gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat
     6721 ttctatgagg caccgcataa atccgaatac ggttcattag acatgtaccg catactaaaa
     6781 acaatgtatg acaacggatt taacgaatac atccgtcctg atcatggaag aatgatctgg
     6841 aatgagacag gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta
     6901 aatggattat gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa
     6961 ctgtaatttt attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg
     7021 ttgttaataa gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct
     7081 tttttcgatt gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc
     7141 ataatcactt ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg
     7201 atatgagaag gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata
     7261 aaatcatttt gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga
     7321 tctttactga tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt
     7381 ttgtagcgat catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct
     7441 aaaataactg gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg
     7501 gcggtttgga cttctttcaa cacgctatgt tcttgataaa gcatgaaagt aggaaggggc
     7561 gtatttggtt gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat
     7621 gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa atacagtaca aaaggactcc
     7681 accatttctc ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc
     7741 tccaagcttg tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact
     7801 aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagggatca
     7861 ataaaaaagt caatatattc atactctgtg aattcttttt gtacgtcttt cacagaagtt
     7921 gcaggaaaat tctctttaca tatttttaat agaaaatata atttatctat cataccgcta
     7981 tttagtcggt caagtacttc ttttttaaaa aaagtattca tcataaaagc cactacttgc
     8041 ggatcttcat cataaaattc tgtagcagta cttgaaaaga tgttccaata aatatctaat
     8101 gctgcatatt gaatttgtac ctcttttcca gaaaaagact ttctgccatt attagaaaag
     8161 gagatagaca aaccgtacaa tgctaaaaat gcattaatct ttttcatttt cgtatacaaa
     8221 taagaggcac tatagtttgt ttgctgactt aaactgataa tcgaatttgt ctgacagctg
     8281 aacaaagaag tcaagatctg ataactgact gattcgttgc agtatcttcc aaatagacga
     8341 taatagcagt aagccggatc aattccttca aaattcaact ttaaaaaagg tttttgctta
     8401 tcaatatgca gttctgttag attgagacgc tcaaagtctt gttctaattc tttgatgtat
     8461 agtaaaaaat ttgattcaga catatggaaa tgatctgcta aatattgtgt agtaataaag
     8521 aaacgatgtt cagataggaa aagaaaaata tctaattttg tattgctttt tttcttcaag
     8581 agaaacgttt taatcgcttc ttgagtttgc atgaaagagg ctccttttta cttaaattgt
     8641 taaaacttga aaatattact gtttttttca taaatttaga caaaaaaaca gtaaacatat
     8701 aggtagaatc tctactatct tatttcttgc atacaaaaaa atcaaggagt gagagagtta
     8761 tgagatattc aaccatctta atatcaacat ttgcagcctt aagcttatcg cttactataa
     8821 atcagacagt ccacacagag gagatagaaa cagccaaatg gacagaaacc aagtgggcca
     8881 acgtcaatcg aattaccttt caggatgaat tttcatcctt caattcagga gtcgcaaaat
     8941 taagaaaagg acaaacagcg accatcaaag caaaaattga ctattcagga gacttagcac
     9001 ctatactaca atcagctgtt acatttgaga atgttgatcc cgctttgtcg attggctatg
     9061 acgagaacac tttgactatc caacacaagc aagcgatatt taccttcacc gttacattaa
     9121 atcaggactt gttaaaacca gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg
     9181 atcatcatca tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagctatcg
     9241 actcaggagg agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag
     9301 aagtaccgcc tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag
     9361 aacccgatac agaaactcaa ccagagccag acaatgaatc aaaacctgaa ataacccctg
     9421 gagaaaaacc agatgtagac ccagaagaaa aaccggatgt gactccagaa ccagatacgg
     9481 attctggaaa ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc
     9541 cagaaaaacc agaggcagcc ccagaagaaa aaccagatgt aactccagaa ccagatacgg
     9601 attctggaaa ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc
     9661 cagaaaaact agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg
     9721 acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa
     9781 aaaaagagag taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata
     9841 aaaaatcacg aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata
     9901 caacctttgc tagtcctgaa acgaaaaata aacagctacc aaaatccgga gagtcacaaa
     9961 acaaagttat actgtggagc ggaatcattc tattaagtat agcaacaatg ctttctgcaa
    10021 aaagatttaa acaaaacaga tctctctaac atttgaataa tgtctgtgaa cgcatttttc
    10081 aaataaaaag aaatatccag aaaacgaccc caaaatacca gattgaaatc tgatatttgg
    10141 ggtcgttcat actttatctt tcttcatatt tattttaagg gatatctgtt tcttttggaa
    10201 gatggttcgg gatctttttc actttcaata catctaaaaa gaaggtaaag atcggaatgc
    10261 cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac aagtaagacc acaaatgtat
    10321 aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg attcagcaca taagactcaa
    10381 ttagatggac aatcaaaatc gtaatcagga tataaacaac atctcggatg cctccttggg
    10441 aataagctat gaaagataga ggaatacaag aaatgataac acctgctact gggatcagac
    10501 ttaaaatgaa aatcatgatc gctaaactca gcagctgatg aaagccaaat acagccaagg
    10561 cgattgtagt gagcaacgtg tttaaaagag cgataacaaa ttgagcttct aataccaaac
    10621 caaatgtatt gacaaactta tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt
    10681 cactttttaa aaacaatctt gagaatacaa ttgttttttt cttttcaatc ataaaaaaga
    10741 aacttaagat gaatgacata gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc
    10801 caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag
    10861 tatggatata ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa aaatcaataa
    10921 cggactggat catatcaaat gtttgatgaa taagcatcgg gatatacttt gtgaatgcca
    10981 gatagataag gtaaacgatc agtgtataaa tcatgatagc taaaacacct gtagggattc
    11041 ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag ataaatgtga
    11101 gcaaaatggt tgtaatcata cttctaacta agaaaagaac aaaaacgaca aataataaaa
    11161 cagtgagacg gcgtattttt tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa
    11221 cctccaaata acaaaaaata aacacgttat tcattatatg atggaatatg aaaataagaa
    11281 agagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca
    11341 taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca
    11401 tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact
    11461 tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga ggagtcagcg
    11521 taacatttac gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg
    11581 ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct
    11641 ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg
    11701 taagattcag cggcttgagg agggaagtgt agtgtctaat gatttcctta gaggcggcat
    11761 ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt
    11821 tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag
    11881 agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc
    11941 taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc
    12001 taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc
    12061 cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa
    12121 accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc
    12181 aatattcaat tcgaatgtac gatcgggatt atgaacttct ccagagcgtc cgcctgtgtt
    12241 gatcattttc gtagaataca tttttttcat aaaattacca cctttaatta tattttatac
    12301 aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat
    12361 aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaaatat
    12421 cagagacaaa aactaatctc atcataaaca ttacatatat ttctcaaaaa gttgttttct
    12481 ttcttcaaaa cttacttcac gatggacctg atgaagcatc cataaataac caaaaggatc
    12541 tctgaacatt gagttagtca caccaaagtc catcatttct gtcatttctt gaatctctac
    12601 acagccattt cgtaatgctt tattatgtgt gtcctcgata tcagagacga tgatattgaa
    12661 ccagatcgta tttggatgtt cagcagtagg agcagtcaaa ccaagagacg tgttttcatc
    12721 cagcatatgg aatctcgtgc catacaaggt aaaaatgact tcattattgc ctctagggta
    12781 atctgttgcc tcaatcagtt gtatttcatc aaagattgtt tgatacagtt ctagtgcttt
    12841 caaactatca tttaatacaa aatctatttc cgttccttta tgcataaaga tctcctctcc
    12901 ttttcagtcg aacagttact agattctttt attataccag ttaaagaaaa cgcattcaaa
    12961 aaaatagcgg atttacttac caactcattc gattggatga taagtaaatc cgcgtcccat
    13021 tacttctgct acgctgctga caatgacaaa ggcacttgga tcgatttctt ctatcaatgg
    13081 tttcattcgg gaaaattcat gatcagtcac aacgatcaat aaaacctctt ttgactttcg
    13141 attatacccg ccttttgcct ctagcagggt aaggccacgt ccaatctcag cacttaagcg
    13201 aaggcggatg tcttccaaat aatgttcact catgaccata acagatttct ttcgattgat
    13261 acctgtttcg atataagtca tgacaattga agtgattgca atagataaaa tagcataaag
    13321 aaactcttct attccaaaaa caaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa
    13381 taagccaatc gaagtattca agtggaaata ttttttaaaa atgaggggag ggatcgtcgt
    13441 accgccgcta gaagagttgt ttttatataa aatcgcaact cctaaagcaa aaatcgcact
    13501 tccaaaaatg acagacaaca accgatctga agtaatcatt gtttctggaa ctattgcaat
    13561 gattaaagga aaaaacaagc ttccgaaaag tactttaaaa aagggttttt tacctaaaaa
    13621 tagaagagct agtaccaaca taacgatatt caacactaaa ataactatag ctcggtcaaa
    13681 tcctaaaaca gcttccagta atattcctag accgctgaca cctccagcag caatatgatg
    13741 aggccctaaa aacatattga tagcgactcc taatatgaag agggcaaaaa taatcttaat
    13801 gtatgtccat ataaattgtc tcgtttcttt catttatcca cttccttaat tcattaataa
    13861 tactcttatc ttattataac ttacttttca ccttaagtta actttaatta ataaaaaaat
    13921 taggctagtc taaattatta tgtttcattc ttctagaata tacttctacg ttaaaaactg
    13981 ttaaaaaact tttactttag aaaattggag aaattgatcg cttaagataa attcaggaca
    14041 ctaaatagta caggaagtag attaaaactt ttaaaagttt taagtctatc tatttggcaa
    14101 gtttaaaaaa taaaaattcg gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt
    14161 gtcttttgtt tctctatcac ttatagtgat gacgtcatat taaaacacaa ggaggaaatc
    14221 aatggataca gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa
    14281 tacaagattt cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct
    14341 ttgtggagat ccatatacca taacaactga aaaatggtat caaaaacaac gaccaatgaa
    14401 aaaatgtcta agtaccaatg ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag
    14461 gcgcttacag tatgcgttcc atgttgttgg ggaagataac acggactgtt tttacggtga
    14521 tcaaggaatt tttccttatc gagaaaatgt gattactgaa ccaaattttt attttcgaat
    14581 accttatttt catcaaatcg atcgattcac tattccagaa tgggtaaaag aaacagtatg
    14641 gtatcaaatt tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac
    14701 tttaccttgg ggaagtaaag atccagaccg agaagacttc ttcgggggag atttgcaagg
    14761 tgtgagggat catttggatt acttgacgga ccttggcgtc aatggcatct atttttgtcc
    14821 catatttaaa gccacatcca atcataaata cgatacgatt gattattatg aggtggatcc
    14881 cgcttttggc gataaaaaac ttttaaaaaa cttgattgat gaagcacata aaagagggat
    14941 acgtatcatg ttcgatgctg tattcaatca tatgggtgtc cattcaccac agtggcaaga
    15001 cgttctcaaa aacggagaaa aatcgatcta taaagactgg ttccacatcc gttttttccc
    15061 agtagattct tatcagatga ctgacttacc agaaactgct gaaaatatcc catatgatac
    15121 ttttgctttt actccattca tgccgaaact aaatacagca aatccagaag tgcaaaaata
    15181 tttgctggat atcgcaactt attggataaa agaattcgat attgatggat ggcgtcttga
    15241 tgttgctaat gaagtcgatc atcatttttg gaaaaaattc agagaagcag ttacagaaat
    15301 caaaccagac atctatatcc ttggagagat ttggcattcc tctcaagctt ggctgcaagg
    15361 ggatgaattc catgcagtga tgaattatgc attcacagat tcgattaaag attattttgc
    15421 aaaaaagaaa atcacagctt cacaaatggt cagtggaatg aatcatcagc aaatgcttta
    15481 cagagatcag gtgaacgaag gaacattcaa cttattagat tctcatgata ctgccaggat
    15541 cttgaccctt tgccaaggta acaaagaatt gatgaagtct gtgatggcct ttatgtttct
    15601 tcaaaaaggg tctccttgta tctattacgg gacagaaata gggatgactg gtgaagatga
    15661 cccggattgc cgcaaatgta tgatatggga gaaggaggaa caagatctag agctattcgg
    15721 ttttataaaa gaacttgttt ctctaaggaa acagttgtct aagattattt ctgaaggttc
    15781 tactcagtgg ctaatagtat atgatcgtga agataagctc tatttcacac gggaactaga
    15841 gggacagatc atctatgttt actttaatca gtcaaaagaa ccttgggtag tagaacagga
    15901 aaatgaggtg attcttagtc aaaattgtca gttgttagaa gacgggaaag cagagatacg
    15961 acaatacgga tttttgatca tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt
    16021 gcaaatgaag atttgtgccc attataataa gggtgtaaca gcacgttgaa tacacaggag
    16081 gaaatgaaga tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat ctctttagga
    16141 atcttaggcg gattagcggc ttgtggcaat ggtaactctt cagcagaagg ggaagccgat
    16201 gaaggaaaaa cgttaacagt ttccgttgat tctggatata aagattatat aaacgaaata
    16261 aaaaacactt tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc
    16321 gatcagttgg aatcattagc gttagatggg cctgctggaa agggacctga cgttatgatg
    16381 gcagcatatg accggatcgg tgcgttagga cagcaaggac atctggcgga agtaaaactg
    16441 ggaaatgaag cagcttacga tgaaacagac aaagcccaag tgacttatga tggaaaaatc
    16501 tacggagaac ctgcagtaat cgagaccctt gttttgtact ataacaaaga tttagtcgat
    16561 acagcaccat caacgtttaa agatttggag aatctatcca aagacagtcg ttttgctttt
    16621 gagtcagaag caggaaaaaa tacaggattt ttagctaaat ggactgactt ttattattcc
    16681 tatggtttaa ttgcaggtta cggtggctat gtatttggtg atgatggcac aaatccttca
    16741 gatatcggtt tgaataatgc aggtgctgta gaaggaatct cttatgcaac agattggttc
    16801 aaaaatgttt ggccaaaggg aatgcaagac ataaaaagtg caggagactt cgccagccag
    16861 caattcatgt ccaataaaac tgcagcaatc attgacggtc catggcaagc gcaaacatac
    16921 aaagaaaaca atatcaatta tggtgtagcc aaaattccta ctctaaataa tggacagcct
    16981 tatcaaccgt ttggcggagg aaaaggatgg gtagtcagca attatgctaa aaacaaagac
    17041 ctctctcaaa aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa
    17101 atggtaaatg aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat
    17161 gaattaacaa cagctgtgat tgaacaatat gaaacagctc aagcgatgcc aaacatccca
    17221 gaaatgggcg aagtttggac aggtgcagaa aacttgatgt tcgatgcagc atcaggttca
    17281 aaaacaccaa aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc catagaacaa
    17341 aaatactgat taaaatgata accgtccatt tttataaaaa atggaacagg tgcttcagat
    17401 aaaagtggca agagcgagat ggaaaattat cttgctcttg ttgccattat cttaaaagca
    17461 gtttgcggtt tagcaagttt agagggggca ttcagatgca gcaagataaa acaagcaata
    17521 tcaacgaaaa aaaaatcacg ctttgttcac ttattccagg gatggggcag tttgtaaatg
    17581 gccaagcttt taaaggtatc gtttttttcg ccatattcgt cctattcatt ggtcagatgc
    17641 ttctgggcgg atttcatgca ttggtaggat tggttacttt gggaagtata ccaatggaag
    17701 atcattcatt atttattttg attgaaggca ctttgcaatt catcattaca atcattttct
    17761 tgatttttta cggtttgaat cttttagacg cttatcgagt agcgaagatg cgtcaagagg
    17821 gcaaaaaaat caatcggacg atgaaggaag tcatacataa tataggtgat gaaggttttc
    17881 cttatctatt gacacttccc gcttatcttc tcatgatttt tacgatcatt ttcccagtcc
    17941 tagttacatt gttcactgca tttactaatt atgacttcaa tcatattcca ccagccagtt
    18001 taattgattg ggtaggggct aaaaactttt ttagtatctt ctttttaagc tcatatcgta
    18061 atactttttt tgcagtcttt tcgtggaccg tgatttggac tttatgtgca acaactttgc
    18121 aaatcaccct tggtattctc acagcggttg ttgctcacca gaaatttatt aaattcaaac
    18181 gtattttcgg tatcattttc ttattaccat gggcggtccc agcttttatt actatcatga
    18241 gtttttcgaa tatattcaac gatagcgtag gtgccatcaa tactcaagtg attcctttca
    18301 taaaccagct tccttttgta gatatcagtg caatctcttg gaaaacggac ccgaactgga
    18361 cgaaagtagc aattatctta gtgcagggct ggttaggatt tccctatatc tatgtgatgg
    18421 ttacaggaat cttacaatcg atttcagaag acttatatga agcagcaaaa atcgacggag
    18481 caaatgcatg gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc
    18541 caacatttat tacacagtat acggggaatt tcaataattt ctccatgatc tatctgttta
    18601 acaatggtgg tcccggaagc gtaggcggag gagctggttc tactgatatt cttatttcct
    18661 ggatctataa acttacgact ggaacttctc cgcagtattc tatggcttca gcaatcacgt
    18721 tgattatctc cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag acaaatgcgt
    18781 tcaatatggg ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact
    18841 ttctcacttt tttacttact tttttctttt aatactgtca gtcattatca tctatccgct
    18901 tttgatcaca gcaagtactg ccttcaagtc aggaaatatt gcagctttta gtttagatct
    18961 ttcaagtgac tggactttag gaaactttac tcggctattc agtgaaacac tttacgggac
    19021 ttggtataaa aatacattga tcatttctgt cctaacaatg atcgtacaag tgactgtagt
    19081 aactttggcg ggctatactt atagtcgcta tcgttttatc ggccgcaaat caagtttgat
    19141 gttcttttta gtgattcaga tggtaccgac tatggcagca ttgactgcct tttatgtcat
    19201 ggctttctta ttagaagcgc tcgataagta ctggttcttg acaatgatct atataggcgg
    19261 tggaattcca atgaatactt ggctgatgaa aggatacttt gacactgtac caatagattt
    19321 agatgaatct gctaaacttg atggggcagg gcatttcagg atttttttcc agattgtatt
    19381 accgcttgtc aaaccgatgc tcgccgttca agcattatgg gcttttatgg gaccttttgg
    19441 ggatttcatg ttagctaagt tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg
    19501 cttacaaaca tttatttctg atgtgacaaa ccaaagagtc tctctttttg ctgctggggc
    19561 aattttgatt gcactaccga tttgtctatt attcttcttt ttacaaaaga actttgttac
    19621 cggcctgaca tctggcggaa caaaaggata ggagcagagt atcatgcaaa catttccgtt
    19681 aaactatttt tccgcattac gtacgccaac tcaagtgttt gcgggacgca aactattatc
    19741 atggcctaaa tttttcttga tatttgtatt tttagtttcc ttgatggtca tgcctgtgac
    19801 tcttttttat gccaatcaga tccaagcgat tccattagaa caatttctat ccgttcattc
    19861 gctgatcgat gaacaaggaa cccaaaaatt ttctgaactt gaattatctg aaactgggtt
    19921 acaagcatcg caacaaacga tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc
    19981 tgagaaacaa caatccgatc atggaacttt tattgatttt gagaaggaac aatgggtgat
    20041 ccaacaaaag gataaatctg gtatacgccg ctatacgatg aattatagcc catctttcca
    20101 accagattct gtacgtacgc ccgaagattt ccaacgattt ttagaaagag aattttatgc
    20161 cagtaatcgt ccaacaatca ttttatctta tagtttaagt cttggtcttg tcctatttgt
    20221 catgacgagt ctcattttat tcggggcttc ctttttcttg tggatgacaa gaaaaagcca
    20281 actttcatcc attcatacat tcaaagagag tgcaaatttg atgctgaatg ttatgggaat
    20341 aggttccatg gcagcagcag ttgtagggtt tatccacttt gactttatct tgatgctaag
    20401 tgtccaaaca acgataactg tcttgctgct tttatgggtt tttgtcaaaa caagattcaa
    20461 agataagcgt gtcgaatgac aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat
    20521 gaaaaataaa gtagtttttg aaaaatttct gaaaaaatta aacttcttag ttgattcttt
    20581 tcttttttag tattaacgta atagggtagt ttttttaagg aggaaaatca tgttagagaa
    20641 aactttatac aatcaattat tttcacgttc tttctcgctc cctgtcgaag taaattattg
    20701 ggacggtaca acgaaacaat acggagatac ggacactcct ccccaaatca aaatcaatat
    20761 ccatgaagaa atcccaatga aagagattac aagtaacgct tctttggcac taggagaagc
    20821 ctatatggat catcgaatcg agatcgaggg aagtatccaa gcattgatca acgatgctta
    20881 tcagcaagca aacagctttt tacgaggaaa agattatcta aaatggctgc caaagaaaga
    20941 aaaacatacg aaacaaaaaa acaaagaaga tatccatgcg cattatgact taggtaacga
    21001 cttttataaa ctttggctag atccaacttt gacttattct tgtgcttatt tcaatacacc
    21061 tgaagatacg ttggaacaag cacaaatcaa taaagtccat catatcttag acaaactctt
    21121 tatcaaagaa ggcgaaacgt tgcttgatat cggttgtggc tggggaacat tgatgttcac
    21181 tgctaccaaa gagtataatg tcaaggcaac tgggatcaca ctaagtgaag aacaatacga
    21241 tttgattacc aaaagaataa aagaagaaca tttagaagat aaatgccgtg tattattaat
    21301 ggattaccgc gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga
    21361 acatgtaggt tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc
    21421 aaaaggaaca gctttgatcc atggaatcag ccgacaacaa ggcggtgcga caaatgcttg
    21481 gatcaataaa tatattttcc ctggcggtta tgtccctggt gttactgaat tagttgataa
    21541 tattacaaaa aacgatttgc aactgattga tttggaaagc ttacgtcgtg actaccaatt
    21601 gacattagaa cattggacaa agaattttca tcaagtccaa gatcaagtca cgaaagaaat
    21661 gggtgattcc ttctatcgaa tgtgggatct atatttacaa gcttgtgcag cttcatttga
    21721 atcaagtaat atcgatgtga ttcaatattt gcttgttcaa cctggaaaca atgacatccc
    21781 aatgcatcgt gcataattga ggcaaaagta ctgtttaaag actatttaaa aagaagctaa
    21841 gttttatgat gctccagaag tgaacttttt tgtctaactt ctaggattgt ctctaaagct
    21901 tagtttcttt ttctttatat tttcataata aaagtttggt tctttaattt ttaaac
//
LOCUS       DAFCHQ010000049        21857 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000049 DAFCHQ010000000
VERSION     DAFCHQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.049"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            87..1091
                     /locus_tag="LNC71_001810"
     CDS             87..1091
                     /locus_tag="LNC71_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466880.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1092..2036
                     /locus_tag="LNC71_001811"
     CDS             1092..2036
                     /locus_tag="LNC71_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6466881.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2036..2998
                     /locus_tag="LNC71_001812"
     CDS             2036..2998
                     /locus_tag="LNC71_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466882.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3025..3939
                     /locus_tag="LNC71_001813"
     CDS             3025..3939
                     /locus_tag="LNC71_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6466883.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3965..5746
                     /locus_tag="LNC71_001814"
     CDS             3965..5746
                     /locus_tag="LNC71_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6466884.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6094..6780
                     /locus_tag="LNC71_001815"
     CDS             6094..6780
                     /locus_tag="LNC71_001815"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBK6466885.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6800..10381
                     /gene="smc"
                     /locus_tag="LNC71_001816"
     CDS             6800..10381
                     /gene="smc"
                     /locus_tag="LNC71_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBK6466886.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10390..11199
                     /locus_tag="LNC71_001817"
     CDS             10390..11199
                     /locus_tag="LNC71_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6466887.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11210..12208
                     /gene="ftsY"
                     /locus_tag="LNC71_001818"
     CDS             11210..12208
                     /gene="ftsY"
                     /locus_tag="LNC71_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBK6466888.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12395..14662
                     /gene="gshAB"
                     /locus_tag="LNC71_001819"
     CDS             12395..14662
                     /gene="gshAB"
                     /locus_tag="LNC71_001819"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBK6466889.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14751..15203)
                     /locus_tag="LNC71_001820"
     CDS             complement(14751..15203)
                     /locus_tag="LNC71_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBK6466890.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15353..16303
                     /locus_tag="LNC71_001821"
     CDS             15353..16303
                     /locus_tag="LNC71_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK6466891.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16296..17255
                     /locus_tag="LNC71_001822"
     CDS             16296..17255
                     /locus_tag="LNC71_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBK6466892.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17252..18007
                     /locus_tag="LNC71_001823"
     CDS             17252..18007
                     /locus_tag="LNC71_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6466893.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18030..18983
                     /locus_tag="LNC71_001824"
     CDS             18030..18983
                     /locus_tag="LNC71_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6466894.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19627..19923)
                     /locus_tag="LNC71_001825"
     CDS             complement(19627..19923)
                     /locus_tag="LNC71_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBK6466895.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20278..20634
                     /locus_tag="LNC71_001826"
     CDS             20278..20634
                     /locus_tag="LNC71_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6466896.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20644..21543
                     /locus_tag="LNC71_001827"
     CDS             20644..21543
                     /locus_tag="LNC71_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBK6466897.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         7225 a         3858 c         4659 g         6115 t
ORIGIN      
        1 acctttgtta taaaacgatt tattctgtgt aatgtccttt tttattaaga aaaacttcca
       61 tttaggaagc agagaggggt attcgattgt ccgataacca attattagaa gttcagaacc
      121 tacatacagg attccgactc aaagatgact attacgatgc agtagatgac gtttcattta
      181 ctttagacaa aaacgagatt ttagcaatcg ttggggaatc tgggtgcggg aaaagtacct
      241 tggctactac gattatggga ttacttgatc ctaataacac aaaaattaca ggtgagattt
      301 tatataacga tctgaattta atcgacctga acgaaacgct ctataataaa atccgcggaa
      361 atgatatcgg aatgattttc caagatccgt tgtctgcatt aaatcctctc atgagaatcg
      421 aagaccagat caaagaagga ctttcctatc acacaaagat gaatgcagaa cagcgtcaag
      481 ctcgtgcact agaattgcta gaacaagtag ggattcctaa tcctgcgaga gttggacgcc
      541 aatatcccca tgaactatca ggcggtatga gacaacgtgt catcatcgca atcgcaatcg
      601 cctgcaaacc tccaatcgtc attgcagatg aaccgacgac agccctagat gtaaccatcc
      661 aagcacagat tcttgattta ctgaaagacc ttcaggaaga aacaaaaaca gggatcatct
      721 tgatcactca tgacttagga gtcgtagcag aaatggctga tcgagtggca gtgatgtatg
      781 gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt tgatcatcct aagcatcctt
      841 atacacgctc tttactgaac tctataccac aagaaggaaa tcatgatgca gaactccatg
      901 tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg taaaggttgt cggtttgcac
      961 cacgtattcc atggatacca gcaagcgatc atgaagaaga gcctatcctt catgaagtcg
     1021 caccgaacca cttggtacgt tgctcctgct ataagcattt ccactttaga gacgaaaaag
     1081 gagaggtgta gatgacagaa ttaatcacaa tcaaagacct gaaagttcat taccctattc
     1141 gcagtggatt cttcaatcga gtaacggatc atgtatatgc tgtagatggt gtggatttta
     1201 ttatcgaaaa aggaaagaca tatggtttag tgggagagtc tggctctgga aaatctacta
     1261 cgggaaaagc cgttgtggga ttagaaaagg taacagccgg acaaatcatg tatgaaggaa
     1321 aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta taacaaggat gtgcagatga
     1381 tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg agtgttggat atcattgctg
     1441 aacccatccg aaattttgaa cggttaagcg atcaagaaga aaagaaaaag gtaaaaggac
     1501 tgctagatat cgttgggatg ccggaagatg cgctatataa atatcctcat gaattttcag
     1561 gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc taccaatcca aaactgatcg
     1621 tagccgatga acctgtatct gctctggatc tatctgttca agcgcaagta ttgaatttca
     1681 tgaaacgaat ccagcaagaa tacggcctca gctatttatt catttcacat gatttaggtg
     1741 tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa aggtcgattt gtagaaatcg
     1801 gtacaagaga agacatttat aatgacccgc gacatatcta tacgaaacgt ctgctttcag
     1861 ctattccgca aatcgatgta gaaaataggg aaagccacaa agaaaatcga cgaagagtag
     1921 aacaagagta tatccaaaac caaaaagaat actatgatgc gactggtcga gtctacgatt
     1981 tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga tggaggtgca agctaatgtg
     2041 gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa gttattattt taagtgttct
     2101 gatcttctta ttagcccaag cgatgccggg ggatccattt accggtttga tcaatccaaa
     2161 tcaggatccg gcagtgatcg aacagatgcg acaagcagct ggcttgaatg acccatggta
     2221 tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc gattttggtc aaagtttctt
     2281 atacaaacgc tctgttgctg acttgatcgg cgaacggatc atcaacactt tatatctatc
     2341 gatcttgaca gttgtcctta cttatttgat tgctgtgcct ctggggatgc ttgctggccg
     2401 gtatcaaaat tcaatcttgg ataagattgt cgtaatctac aacttcttta gttttgctgt
     2461 tcctttattt atcttcggct tgatcatgct atttgttttt ggttatcgtt taggctggtt
     2521 cccaacaagt ggatcacaaa cactaggttt gcaaggtgga tttatcactc aatggataga
     2581 ccgtttgaaa tatattctgt taccatcaat tacccaagca tttttggcga ctgccgtcac
     2641 aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg ctcgattttg tcagaacagc
     2701 ccgttccaaa ggggtaccga cgaataaagt attcagtcga catattttcc gaaatgcagc
     2761 gttgccgatg gcttctcaaa tggggtatga aatcacttcg ttgattgctg gttctgtagt
     2821 tatcgaaaag atttttggtt atccgggagt cggaaaacta tttattgatt ccatcggcca
     2881 acgggactat accgtgatca catccttagt attgatttta ggaatcgcga cgctagtggg
     2941 gacattactt tcagatatca tcatgagtat cgttgacccg cgtattcgga ttcaataaaa
     3001 ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca atacagcagt tcaagaaagt
     3061 attcctccta tggggatacg aatgattgct cgagaattta aaaaagataa aattgctatt
     3121 ttttcattag gtttgctagt ggttttatta ttagtgatct ttatcggtgc gatgctgacc
     3181 gatcaagaca aagtgatgta tgttagtatc ttcgacaaat acgctgcacc aggaacagtt
     3241 tcgacacaag gacaaaagtt cattctggga gcagatgaag gcggacgtga tgtattagga
     3301 caattgatca taggtgctag aaactctgtt acgatcggat ttgcgatcac gatccttact
     3361 tcgatcatcg gggttggatt agggatcatc tctggctact acggagggat cattgacaac
     3421 atcttgatgc ggatcgttga ttttatcatg attctaccga tcatgctgat cattatcgtc
     3481 ttcgtatcga ttatttccag atacaatgtc tggtccttta tattcatcat gagtgcattt
     3541 tattgggtag ctaaagcccg attattccga agcaagacat tatctgaagc aagaagagat
     3601 tatgtcagtg cctcaaaaac aatgggaacc aatgatttta aaatcatgtt ccgcgaaatc
     3661 atgccgaatc taagttcatt aatcattact aatttgacga tgaactttgc ggcaaacatc
     3721 ggtatcgaga caactttgac tttcttaggt ttcggcttgc cggcaaatgt acctagttta
     3781 ggtacattga tcggttatgc aagtaacgga gaagtcttag tgaatcggca atggatctgg
     3841 ttacccgcat caatccttat tttagttttg atgttgagta tcaattatgt tggacaagca
     3901 ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt atagaagcga agggggaaat
     3961 aggaatgaag aaaacgattt ttggatttgc agcgctgatg tctgctgtcg ttcttgcagc
     4021 atgcgggggc ggaggtacag atacctctgg aaacaactct ggaggcaacg gcaataacaa
     4081 agaggaatta acttttccaa ttgcaacaaa aaataatgat gatgcaatcg aagacggcga
     4141 attagaagct gcggtcgcaa cggacagtca gtttaaagga ttgttccagt gggagtttta
     4201 ccaagattca tatgatgcag cttttatggc tccttctcat gaaccgctat ttgcaaacga
     4261 tgacgatttc agaatcacag acgacggagc tgcaacgctt gatctagatg aagatacgaa
     4321 aaaagcgaca atcaaagtaa aagacaacgt caaatggtca gatggtgaag atgtaacggc
     4381 ggacgatgtg attttccctt acgaaatcat tgggaataaa gattacactg gtatccgtta
     4441 tgacgacact ttccgtaaca tcgtaggtat ggaagaatat catgatggaa gtgccgacac
     4501 aatctccgga atcaaaaaaa tagacgacaa aacagttgaa atcgaatata aagaagtaaa
     4561 cccaagcatg cttcaatcgg gtggtggtat ctggtcatat gcagcaccaa aacatacgtt
     4621 agaaggtgta gcaatcaaag atatggaatc aagcgatcaa gtacgtaaaa atccagtgac
     4681 ttttggccct tattacatga gtaatatcgt tgcaggagaa tcagtagaat acttgccaaa
     4741 cgagcactac tggaatggta cgcctaaatt gaaaaagatc acgatgaaat ctgtaccgat
     4801 ggcaagtgca actgaagcat tgaactctaa attatatgat atgattttcc aaatgccaac
     4861 agatacttac gatacctata aagaaattgc tggctataca aacttaggcc gtgaacaaac
     4921 ttcttatact tatctaggat tcaagttagg taaatggaat gcagatacaa agagtgtgga
     4981 atacaatcca gattcaaaaa tggctgataa atcattacgt caagcaatgg gctatgcatt
     5041 agataataat gcagtaggtg aacgtttcta tgcaggtttg agaagcaatg cgacttcatt
     5101 gattcctcca gtattccagt cataccatga tgatgagatg aaaggattca cacaagatct
     5161 agacaaagcg aaaaaactat tagatgatgc tggctacaaa gatgtggatg acgatggttt
     5221 aagagaagac aaagatggaa ataaactaac aatcaacttt gcttcgatgt ccggtgggga
     5281 aacagcgcaa ccattggcag attactatat ccaacaatgg aaagaaattg gtttggatgt
     5341 ccaatacaca acaggtcgtc tgatcgaatt caattctttc tatgataaat tagaaaacga
     5401 tgatcctgaa attgatgttt accaagctgc ttggaataca ggaactgatc caaatccaag
     5461 tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt tatgaatcag aagaaaatac
     5521 aaaattgatc aacgatatca gttcaagcaa agcatttgat gaagagtacc aaaaagaagc
     5581 atatcaaaaa tggcaacaat atgcatttga agaagcattt gcgattccaa cgttgttccg
     5641 taatgaagta ttgccagtca acgaccgtgt caaagactgg gattggtctt atgatgtacc
     5701 aaacccatgg gcaaatgttt ctgttacagc agattcacgt tcataatcac taaaaatgac
     5761 cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt tctgtttatt tctctaatat
     5821 tcaggtcaag gcgctgattt catgacttct ccaataaacg gtgttcaaag tccagtttct
     5881 cggtattaag tcaaatttat gcaaaaatat ggaaagccat tttcacaaaa ttttttcttc
     5941 atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa tcactaggat tattaaagct
     6001 ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa aaccagtata ataaggaagt
     6061 atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca accagctgac aaaagaacta
     6121 aaagaaaact ataacatcgt ttttcacgat ctaaatttgc ttgaacaagc ttttacccat
     6181 tcatcctatg tgaatgagca tcgcaatctg caattatcgg ataatgagcg acttgaattt
     6241 ttaggagatg ccgttttaga actgatggtt tctcagtatt tattccgttt atatcctcat
     6301 ttgccagaag gaaaattgac aaaaacacga gctgcgatcg ttcgcgaaga cagcttgtcg
     6361 aaatttgcca aagaatgtca ctttgatcaa tatgttttgt tgggcaaggg agaagagaat
     6421 tcaggaggac gtacgcgacc agcattgctt tgcgacctat tcgaagcttt tttaggcgcg
     6481 ttatatcttg accagggatt cgacacgacc catgcattca tcgaaaaagt gatttttcct
     6541 aaagtcaaag caggtgcttt ttcacatgag atggatcata aaacaaaact acaagaagtc
     6601 ttgcaaaaat caggtgatgt atcaatcgaa taccgattga tcaatgaaga aggtccagct
     6661 catgaacgag tattttggat cgaagtttat gtagatgatc aattgattgg cactggacaa
     6721 gggaaatcga aaaaattagc agaacaagct gcggcggaaa atgcattggc agcactgtaa
     6781 aagtaagtga gggaaagctt tgtatttgaa acgaattgaa atagccggtt ttaaatcatt
     6841 tgcagacaag acagtcattg attttgaaaa tagcgtcaca gcagtggttg gaccaaatgg
     6901 cagcggaaaa agcaatatta cagaggctat ccgctgggta ttaggagaac aatctgctaa
     6961 aagtctgcga ggcggaaaaa tgccggatat catctttgcc ggttccgata cccgtaagca
     7021 gctgaatatt gcagaagtca cagtgatttt agacaatagt gatcattatc tgccattgga
     7081 gtacaatgag atcagtgtaa cccgccgtta tcgacggact ggggaaagcg agtttttcat
     7141 caataaacaa agctgcagat taaaagatat ccaagaattg ttcttagatt ctggattagg
     7201 aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa gcgattttca gtagtaaacc
     7261 ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc ttgaaataca agcagcggaa
     7321 aaagaaagca gaacagaagc tttttgaaac agaagacaac ctcagccgcg tccaagacat
     7381 catccacgaa ttggaagaac agttaactcc tttggccgcc cagagtgagg cagcaaagga
     7441 gtttttacgg ctaaaagaaa cactgactca aaccgatgtc tctttgatgg ttgctgaaat
     7501 caagacagca aaaaaagatt gggacaacaa acaagcacag cttgcgaaat tcaatctaga
     7561 attaggcaaa ctatctgagt cgatccaaga acaagaatcc attttggcga agcagcgtaa
     7621 agaaaatgcg caagccgatc gtctgatcga aaaaaatcag caagtattat tagatctttc
     7681 ggaaaaactg aagcagacag aaggacaaaa agatgttttg caggagcgga caaagcatac
     7741 acaaaaaagc tcgcaagaat atcaaacttc attagcagaa gcacaaaaaa aggttaaaca
     7801 ttttgaaaag cttcaagaaa gcttgatgaa agcagcagca gaaaaagaaa cagagatcca
     7861 aaaagctgaa gcaaatctca taaaaacaca gcaagaatta gaaaaatacc aaaaatcaac
     7921 gaaagaatta ttggcagaat taagagatca atatgttgat ttgatgcaag aacaagcagc
     7981 tgtaggaaac gaactgaagt atctagaacg tcaatatata caagaaacag caaaaagcaa
     8041 gcagacgtta gccaagcaat cggaagtaga agcatcagta gaccgattga tcttacaaaa
     8101 agaagaattg acacaaaagc aggcgcagct aaaatcatct ttaactgaaa caaaagagaa
     8161 gttagaaatg atccaacaaa acgggaaaaa gttccaagaa gcattagcaa aagaacagcc
     8221 taaaatgtat caattaatga atcaagtcca gcagctacgt gctcgtaaga aaagccttca
     8281 agaaatccaa gaaaactatt ttggttttta ccaaggggtt cgattggtct tgcagcataa
     8341 acagcagctt tcgggtattg tcggagcagt ggctgaactg atcgatgttc ctgcagattt
     8401 cacgttagct atcgaagcag ctttaggtgg ggcagcccag catgtcatcg ttgaaaatga
     8461 aaaagatgct cggcaagcca tcacgtattt gaaacagcaa agaggaggaa gagcaacatt
     8521 ccttccactg acgacaatca aaccaagaca attaccggct catatattga cacaagcagc
     8581 agctgtagaa gggtttatag ggattgccag tgaacaagtc agttatcccg atcaaatcca
     8641 aactgtggtt caaaacctgc taggaacgat ccttttagcc aaagacttaa ctagtgccaa
     8701 tgcaatcgcg caaacgatcc gctatcaata tcgagtggtt tccttagaag gtgatgtgat
     8761 gaatgccgga ggatccatga caggtggagc gaataaacga ggaaaccaag gcagtttgtt
     8821 tgtacaaaat caagaattga aacagttgac tagtgaattt gaagaagcag ataaacaact
     8881 ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa acagctcgtt tagccgaaga
     8941 acaagaagtt ttgcgtacac gaggcgaaca attacgtttt gaagaacaag aagcgacaaa
     9001 tcagctacaa aacatcataa atgaacttga acgttttgaa aaagaaaagc aaatctcaac
     9061 ttttgagaca cgtgaactgc agcagtttat cgaagattac gaaaagcaaa cgaacgaatt
     9121 gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa atcgacgaag aaatcaaatc
     9181 gttaagtcaa gaaagcgacc agatggaagc cagaagagca caagtacaaa gtcaaaaagc
     9241 acaagaacaa gcagatcttg ctgtcttgaa agagcagttt aatcatctgc agatccagct
     9301 ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt caagaagcga tagaaaagca
     9361 gttggcgaca ttgacagcag acttttctga tcatgaagtc acagaagaaa gtttggaaaa
     9421 acaaattaac gaattatctg ctcagcgaga aacattaaaa gccgagttgg caaaagccaa
     9481 agagcagcga gatagaaggc aaaaggagat cgataagctt gaagcagtct tggcagagcg
     9541 taatcgtgag caaaaagcac gactgtcgga acaatccaaa ttagaagtcc aaaaagaccg
     9601 agctgaaatg attttagata atcatttgac ctatctgcag tcagaatatc agctgacatt
     9661 tgagaaagct tcccaagact atcaagaaac aaccgatatc gaagacagta gaacaaaggt
     9721 ttcatcctta aaagaacaga ttgaaaaact gggaccagta aacttaaatt caatcgaaca
     9781 atacgaacaa gtcagtgaaa gacatacatt cttagcaaca cagcgagatg atctgttagc
     9841 agcgaaaaac caattgtttg aaacgatgga tgagatggat gatgaagtgc ggacaagatt
     9901 caaagaagta tttgaagcta tccgtcaaga attcaaaatt gtctttccaa atatgtttgg
     9961 cggcggtaaa gctgaattag tactgacgga accaactgat ttgctgaaaa caggtattga
    10021 gattgaagta caacctcctg gcaaaaaact gcaaagtcta agtttgcttt ctggaggaga
    10081 acgagcactt acagccatcg cgttgctctt ttccattatc cgtgtctgtc ctgttccgtt
    10141 ttgtatctta gatgaggtag aagcagcgtt agatgaagcc aacgtgaaac gttttggtcg
    10201 ttacttgagc gatttccaag atgacacaca atttatcgta gtcacacacc gcaaaggaac
    10261 gatggtcgca gcagatgtac tgtacggagt gacgatgcag gaatccggtg tatcaaaaat
    10321 cgtttctgta cggatggaag atatcaatga agaggcgaaa atcaacgaaa cgaaagtgta
    10381 ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg gtacattatt aaatgaacaa
    10441 aagctgattt cagatgagaa taaacaagca ttagcacaag caaaacagca aggggtcaag
    10501 atcgttctat gtacaggacg ccccttagct gcgatggcgc attatctgca agaattagga
    10561 ctggtagatg aaggtgacta cagcatcact ttcaacggcg gtttggtaca aaaaaatgat
    10621 actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag atatccaacg cttatacaaa
    10681 ctggctcagc aattggatct gccactagat gttttgtctg acaatgtggt tttacagctg
    10741 cctagtgccc caaggaagca atccttgtac aatgtgttga ataagctgct gcaattccaa
    10801 ccagcggata taagcgatat tacagatgaa tttgtgctta ataaggcagt aatcgcttat
    10861 gaccaagaag aattagatcc taaaatcaaa gaaataccag cagaattcca tgaaatgtat
    10921 gagatcatca aaacaagaag tatgcttttg gaatttatgc caaaaggtgt tacaaaagct
    10981 tatggtattt ctttattggc aaaagatctt ggtttgaaac aagaagaaat catggcgatc
    11041 ggtgatgagg aaaatgattt accgatgatc caatatgctg gactaggcgt agcgatggag
    11101 aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga cagccagcaa tgaagaaaat
    11161 ggcgtagcca aagtgatcca aaaatttgta ttagagtagg gggagtcaaa tgggattttt
    11221 tgataaaatc aagcgtgctt ttactggcga agagaaaaca gaagaagttg aagtacagga
    11281 gaagtacgac aagggtttag aaaagacgcg taaaacattt ggtgaacgga tgaatgagtt
    11341 gtttgccaat tttcgaacag tcgatgaaga cttttttgaa gaattagaag aaacacttat
    11401 cggtgcagat gtaggttttg aaacagcgat acgaatcacc gaagcattac gtcaagaagt
    11461 gaaactcaga aatgcgaaaa aatcggcaga agtccaaaat gcgatcatcg agaaacttgt
    11521 ggatctctat gaagcggaag gaatcaatga agtaaatgaa ctaaatatcc aagaagacgg
    11581 actaacagtt attttgtttg tcggtgttaa tggtgtaggg aaaacaacaa gtattggtaa
    11641 acttgcccat cagttcaaaa acgaaggtaa aaaagtattg atggcagcag cagatacctt
    11701 ccgcgctgga gcaatcgacc agttagttgt ctggggcgaa cgcgctggtg ttgaagttgt
    11761 tcgtggcaat gcaggaggcg atccagcagc tgtcgtattc gatgctttgg aaagagcaaa
    11821 gacagagcag gcagatgttt tactagttga tactgcaggc cgactgcaaa ataaagtgaa
    11881 cttgatgaac gaattagaaa aaataaaacg agtaatccag cgggaattac cagaagcacc
    11941 ccaagaaaca cttcttgtat tagatgcaac cactggtcaa aacgcgatgg ttcaagcaaa
    12001 acaattcaaa gaaacaacag atgtgaccgg tcttgttttg acaaaattag atggtacagc
    12061 aaagggcggt atcgtacttg ctatccgtaa tgaattgcat ttaccagtga aattggtcgg
    12121 tcttggtgaa ggaatcgatg acttggagcc gtttgatcca aatgattttg tcgttggatt
    12181 gttcaaagga ttactaaaag aagaataacc catgtctaaa aagcaggagc tgtgacaaaa
    12241 gttgcagctc cttgtttgaa tgattgtttt taacatagct gtgtttttga tgccttcatt
    12301 tttatactta tttgtaagcg gttctcaaac aatttcgttc tttttcttag actttttgat
    12361 tataattaga ttcatatgaa ttggaggtca cttaatgatg aattttaagc aattattatt
    12421 gcatgtcaat gcgcgccctt ttatcgacca agctcgattt ggtatagaac gggaaggaca
    12481 gcgggttgat cttgcaggaa atctagcaaa aaccgatcat ccagcaatct ttggcgatcg
    12541 atcctatcac ccctatatcc aaacagattt tagcgaaaca caaacagaga tgatcacccc
    12601 tgttaccgat tctattcccg aattatttca gtatctcgct gctgtttatg atgtgactgc
    12661 tcgttctata ccgaaagaag agatgatctg gccattaagt atgccacctg ccttaccgga
    12721 aaaagacgaa gagatcatta ttgcaaaatt aaaaaatttc gaagatgtct tgtatcgacg
    12781 ttatttagca aaagaatacg ggaagcgaaa acagatggta agcggtattc atttcaattt
    12841 tgaatttggc gatgagttgc taagaacatt gttcagccat caggaagaat ttcaagattt
    12901 ttccgaattc aaaacagaac tttatttgaa aacagcgaga aactttatgc gctatcgctg
    12961 gatgatcact tatttattcg gtgcttcacc aatgagtgag aagaactact ttttagatga
    13021 atcacatccg caagaacctg ttcgcagtat tcgcaacagt gcgctgggct atacgaatca
    13081 tccaaatgtg aaagtttcct atgcttctat gaaacagtat ttagcagata tcgagcgaat
    13141 gatcgaagaa gggaaacttt cggaggagaa agaattttat acaccgcttc gtttccgagg
    13201 cggaaagaaa gtcgcagatt tagcaacaac aggtgttcgt tatattgaat tgcggaatat
    13261 cgatttgaat ccttatgcaa gattgggtat caatccagag caagttcggt tcttacaatt
    13321 attcctgatg tacatgttat ggacagaaga aaaagaagac tgtgaccaat gggtagcaga
    13381 aggtacgact cgaaataaca aagtagcact agaacaacca tcagatcaaa cagaatttca
    13441 tcaagaaggt agagaaattc ttgaaggaat gaagcaaatg ctggttgaat tggattggtt
    13501 ggattctctt tatttagtag aggaagcatt gactcaaatg gatcatcctg aacaaacgtt
    13561 agcagcgaaa ctctatcaag aagcacagct atcgagtcag caagaagttg ctgtggcatt
    13621 gggacatcaa tattataaag aaagccatga acgtccctat cagttggccg gttttcgcga
    13681 aatggaactt tctactcaga tatttatgtt cgatgccatc caaaaaggtg tccaagtcaa
    13741 agtattagat gagtcggatc aatttttgcg tctgcaattc caagaccatg tagaatatgt
    13801 gaaaaatgca aacatgacaa gtaaggacag ctatatcgtc ccacttatca tggaaaacaa
    13861 aacagtaacg aaaaaagtct taaaagaagc tgggttccgg gtaccaggag gcgcagaatt
    13921 ttcatccatg gaagaagcgg taaaagctta tccgagattt gccgagcagg catttgtgat
    13981 caaacctaaa tcaaccaatt acgggttagg tatcacgatt ttcaaggaag gagctagtct
    14041 ggaagattat caagcaggtt tagccattgc ttttcgtgaa gacagttcgg ttttagtaga
    14101 agaatttatg ccgggaacgg aatatcgttt ctttgtgatc gacggagaag tccaagccat
    14161 catgttgcga gtccctgcta atgttattgg tgacagtatc cgaacagtga aagaacttgt
    14221 tgaagaaaaa aatagtgacc ctttgcgtgg aacgaatcat cgtgcacctt tagaattgat
    14281 ccaattaggt gaattagaac aattgatgtt gaaagaacaa gggttgacga tcgaatcagt
    14341 tccccaagcc aatcaaattg tttatctaag ggagaactca aatatcagta caggtggtga
    14401 ttcgatcgat atgactgatg aattttctga agcttacaaa aaaatcgccg tttctgcggt
    14461 agaagcacta ggagctaaga ttagtggtat cgatttgatt ataccagata aagaaatcga
    14521 tcctacaact gataaaaaag catatgggat catcgaagca aatttcaatc cagccatgca
    14581 tatgcatgtt tatccttttg caggaaaagg cagaagattg acgatgaatg tattgaaatt
    14641 gttatatcct gaagtatttt aaaagaaaaa gcttttgaca gattgtccta tttgatgtca
    14701 atctgatcaa aagctttttt tgcatgtaaa aatttatgtg ttttttcgtt ttaaaataaa
    14761 cgatcccaga agcttttttt cttttttggt ttttcttttt cagctggcga agagttttct
    14821 tctgggtatt ttttttcgat atcaatcaca ggttcattta ctgcttcttt tgctgttaag
    14881 agcaatccga attcctcagg atttgcagtg acgtaatcgt tgactacagt gaaacggcta
    14941 ttgtttttcg ttgctagctg gatatattgg ttttggacag aaaggggtag agccccattc
    15001 aagagtatgg aagcatctgg atgttttttg agttctttta aaaagttttc tttgtttttt
    15061 ggttggatca tttcagcaac agtcatacta agatagcagc gttccctgaa tgtccctaaa
    15121 tatttatgct gttcttcagg gttcactaaa ggtgtaccat aaagtccctt atccaaatgg
    15181 ctttttacat catcttgtgc cataaaaaat accctccttc ttctctttgt ctttcttacg
    15241 attatagcat attcccaata atacctaaaa catatgaaaa agcggttgct tttgttcaac
    15301 gattcactta aaataagaat gaaaatcatt ctcaattaga aaggtggatt ggatgcgcta
    15361 cggacgacta gtagcattat tagttatttt aagtagtatt tcattgttca tcggagtatc
    15421 gaatgtacaa ttgtcagaac tgctccagtt agatgggcaa agcttacaaa tcttgctagt
    15481 gactcgtttg ccacgaacga tttgtttgat ccttgtggga gcgaccagta gtatttgtgg
    15541 gttgatcatg caacatttga cccaaaataa atttgtttca ccaacgactg cgggcacgat
    15601 ggatagtgcg cgtttaggta ttttagtggc tatgattttc ctacctggtg catccttgat
    15661 tgtccgatcc ttgacagcat tttgctttgc ttttgcagga acattgcttt ttttatccct
    15721 tacgcgattg ttcctccaga aaaatcaatt gatgcttccg ttgattggag tcatgttagg
    15781 gaatatcatt ggttcagttg ctactttttt tgcttatcaa ttccagttgg tacaaaatat
    15841 gacttcatgg cttcaaggga attttgcgac tgtgatacga ggaaattatg agttattgta
    15901 tgctgttgtt ccattgttgc tcttggtttg gctatttgct taccgattta cgatcattgg
    15961 tctgggcgaa tcgtttgcag taaatctggg aatagattac cagaagatgc agtttttagg
    16021 cattggctta gtatcattgg ctagtgcagt catgttgatc atggtaggaa gcattccttt
    16081 tatcggagtg atcattccga atcttgtctc tagaatatat ggagatcaag tgcacaaaac
    16141 aattggcgta actgctttag caggtagttt gttcttagta ttttgcgatc ttgtcgctcg
    16201 cgtgttgatt tttccttatg aaataccagt cagtgtgatc gttggaatca tcggtggggc
    16261 tatcttcctt tatcttctga taaggggacg acgatatgct taaaaaaaag ccatctattt
    16321 ttattggtat gcttgttctt gttttatttg ttacaggaat gtacttgacg atccatacat
    16381 atggaaactg gcattttgca tgtgcattga ggagtaagaa aatttttgca tttatccttg
    16441 tctcgctagc tgtcagcagt gccacaatca gctttcagac aattacgcat aaccagtttt
    16501 tgactccttc agtattggga cttgagtcgt tatatgtcct tgtccaaacg gtccttttct
    16561 tctttatcgg aggagtgacg atgcttcagc agacaggtac agtttttttc ttattgaacg
    16621 ttgggctgat ggtcgggctg agtctattgt tatttttctt tcttttgaaa aaaatgagag
    16681 cgaatctttt tttattactg atgtcaggga tgattttagg tacgcttttt ggtagtgtca
    16741 gtaccttttt acaagtcatc atggatccga atgaatatga tttgcttcaa ggcagattat
    16801 ttgcaagctt tggcaatatc cagagtcagt atcttttgcc tgcttctttg atcattatcg
    16861 gctgctggta cctgttattt cgatatcgac atgtgctaga cgttttccat ctcggaaatg
    16921 aacaagcagt aaacctcggg atagacacag atcgttttga atttttgatt ttatgttgtg
    16981 tcagtctttt gacagggaca tcaacggctc ttgtgggtcc agttactttt ttagggttta
    17041 ttgtcgcaaa tatcagttat cgtgtctttt ctacttatcg tcatggtcag ctatttatcg
    17101 gaagctttct ggtagctatg ctgtttctga caggaggaca gctcgcagta gagcagattt
    17161 tcggctggaa tacaacagtg agtgtcgtcg ttgaatttgt cggaggaatt tattttgtca
    17221 caaaattgat tcgcgaaagg aagagtatcc tatgattgat ttacaaaagg tcaataaact
    17281 gtaccatgga aaatcagtgg ttaaagatgt acagttaacg attcctgaaa agaagttgac
    17341 tgcttttatc ggtccaaatg gtgctggaaa gtcaacggtg ttgtcgatga tcagtcgttt
    17401 gataccaaaa gatacaggtg aaatctattt agaccataac gaagtaaagg cttggaaatc
    17461 caatgaatta gccaaaaaat tatcgatcct acgacaatcg aatgaaataa atgtccaaat
    17521 caccgtgaaa gaattagtag aatttggacg atttccttat acgaaaggaa aattgacgac
    17581 aaaagacgag gaaatcgttc aggaatcact ggaacacatt gggttagaag aaatagcgga
    17641 tcaatcgatt gacactcttt caggcggtca actccaacgt gcgtatattg caatggtgct
    17701 agcgcaagac acggattata ttttactgga tgaacctttg aacaatctag atatgaattt
    17761 tgcggttcaa atcatgcaga ttttaaaaaa tttagtacaa gaaatcggga aaacaattgt
    17821 tattgttctt catgatatca actttgctgc aagttatgca gacgaaattg ttgcaatgaa
    17881 agatggacag ttatttacgc aaggaacgac ccaagaaatg ataaaaaaag atgtattgga
    17941 tgaattatac gaaatggaca tacggatttg tgagattgaa ggccgcagat tctgtcttta
    18001 tttctaaaac taacattagg agagaagaga tgaaaaaatt agtagtaggt atcttgattc
    18061 tagcatcatt tggtttagca gcatgtggaa atagcaacaa caccagtcag gcagacacaa
    18121 aaaaaagcag cacacaaact gaaactacat tgacgataac agatagtaat ggagaccaaa
    18181 tcgaggtgcc aaataatcca gaaaaagtcg tcgtattcga caatggttct ttagatacga
    18241 tggatgctct tggtgttgga gataaggtag ttggagcagc tacaagcagc cttcctgagt
    18301 atctttcatc ctataaaaaa gtagaatcag caggtggaat aaaagaacca gacttagaaa
    18361 aaattaatca gcttcagcca gatctaatca tcatttctgg ccgacaaaga gattttcaaa
    18421 aagatctaag tcaaattgct ccgaccattt tcctttcttt agatgcgaaa aatccatggg
    18481 aatctttcca gcaaaatgtc actgctttag gggagatctt tggtaagcaa gaagaagcta
    18541 aaactcaatt agaggaatta tctagtgcaa tcgatcagac gaaaaagaaa gcagaagcta
    18601 ctgacaaaaa agcactagtc acacttgtga atgaagggca attatcagcc tacggatctg
    18661 gttctagatt cggtttcatc catgatttat ttggctttga acaagcagat gatcaaatcg
    18721 aagcatccac tcacggtcaa agtgtttctt atgaatatgt gctggaaaaa aatccggata
    18781 tcttatttgt agtagacaga acaaaagcta ttggtggaga cgacagcaaa gatgatattt
    18841 ctgccaatga actggtagca caaacgaatg ctggtaaaaa tcagcagatc atctcccttg
    18901 aaccagatgt ctggtattta agcgggggcg gtttagaatc aatgaaactg atgatcgaag
    18961 atgtgaatca agcatttaaa tagcataaat aacagaggat taaactctca tactatgtaa
    19021 caataatgtt tcttacattc ttaaatagca agagcctatc ctcaaagaag ttatcaaaaa
    19081 ccatccgtaa aatcgtattg atgattttac ggatggtttt tatttatttg agaaatcttt
    19141 tttcaacgtg aattcattat ctacatcata cgggttgatt ctagaatggg gagtggcaaa
    19201 aaatagtgtg atccattttt tcagcatcaa gatcgcctcc tttgatttga atcaaaggta
    19261 caaagcgaaa cttagataaa acttaaattt agatattttt attttgtacc gatctcttct
    19321 ttaaacgatg ttccatctgc tgcttctcgg caataagtcc actagactag aaaatatgag
    19381 aagctatttt ctgtccagcg ttcttattac tcaaaacaaa tcgcagttgt cacaatctct
    19441 tctaaacgat gttccatctg ctgcttctcg gcaataagcc cactagacta gaaaatatga
    19501 gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg tcacaatctc
    19561 ttctaaacaa tgttcaatca gctgaccttc ggtattagct caaagcactg atttcacaac
    19621 ctctatttag gcatgtgtct cagcaagcgg ttgatagttg atgatctgca tttttcgata
    19681 aaaatcggcg ggaagcttac tccaccgttt tagtaattga tagaaataac tctcttctgc
    19741 tttgatttta tactcgctta atatcaatcc aaaactattc gttttttttg aagctaattt
    19801 tttataaata tccaatgctt tggttttatc ttcattgaca aagagtgttt ttacttcaat
    19861 ataagaaccc tcgatatttt gtgtttcttc gataaaatag atgtttttta tatattcctt
    19921 catagtgatt cctccaaaga agtcttttat ttgataagtt tactctaccg aagagttgtt
    19981 aataatccgt gaacaaaaag taattctttt tccaatagtt gttaaaggtt ttttattttt
    20041 tatagtattt tttgttattt aagaaaggaa taaggttaaa caaataacag gttgcttttt
    20101 gggtcttttt ccgttcaaga aactgaacga agaaaaggga aagttgtctc ttttgtgata
    20161 agagaaatca taataaacga atgacttact ttccaataaa ttgagaacag tcagaaccag
    20221 acaaattagt tgcatctgat gaataaagtg attattctat gacatgaggt gatacagatg
    20281 aaattagaag tgacaaaaga agcacaagaa gtattgaaga ataaattaga agcagacgat
    20341 caacttttat tagatatcga aaacggtgac ggtccatttg cagaaagcca agtcagttgc
    20401 caattggata cggcatttcg attgatcatt gtcaaaaaag atacacaaac agatctaagt
    20461 ctttattctg tagtggctga tacaccagtt ggaccaatca aaatcaaaga cagtgcaata
    20521 ctttacatgg atgatccatc cacacttgct ttagaaccaa cttataatag tttacaatta
    20581 aaaggaccaa gcggcttgag aaaaggcaac ttgcaagttg ttcgaaaaga ctagagggat
    20641 aaaatgggaa aattggcgat catcagcgac ctacatgtgg acatcaataa attcggcgaa
    20701 tcagagctga tgcagctttg gcaagttttg caaaagcaca agatcactcg gcttcatcta
    20761 gccggagata cggctaataa agtagaccgt tgcctagctg ttgtcgaatt tttcgtcaat
    20821 aaaggattat ctacgacatt tcatttcggc aatcacgagt tggcagatgt gcccaatgaa
    20881 cagatgatgg accattttcc agacccacat tttttaaatg aacggtatat tgctttaaat
    20941 gagcagaccg ttcttttagg gatgaatggc tggtacgact atcagttttc cgaattgaaa
    21001 gattacgagg caatcgtacg aatgaagcgt ttgtattggt atgatcggtt catccagcgt
    21061 gggaaaacag atctcgaggt caatgcagaa atgcttgcag ctgcaaaaaa tttgctagac
    21121 cagctggaag caaaaaaact tgaggtgatt ttatccactc atttcgttcc taaacgggca
    21181 tttatcatgt atcaatctgc tccttatgag cgttggaaca aattaaatgc atttttagga
    21241 tcagaatcat tcggagaatt acttgaccgt tattcaaata tcaaacaagt agtttttggc
    21301 catacgcatc gtcgctttga agataaatcc atcggtcaca cgatttacag ctgcagacct
    21361 tttggttatt attatgaatg gcagctgacc cgagattttg tcttgaaaga acagttgcta
    21421 gacagctatg atcctatgaa attacgtgtc ctcttgcgtg cccatcaccc agcttttcag
    21481 gcctatcaaa cacaacattt gcaagaggaa tttgaacaag cgatgacaat cgttccgtat
    21541 tgagacagca ttagtaatgt gtaaaagaaa ataccttgta aaacataagg ataagtaaga
    21601 gcagtagatc catttatcgt ggatatgctg ctttttttgc ggaaaataat cagcgaatgt
    21661 ttatttgatc ggatgatttg taactcgcag agtactagat gcaagcgtgt attctttttt
    21721 tgaacgataa tcacaaaaaa aaatataata tacatttttt atttaattat aattaatatt
    21781 gattgaagtc taggcagtca agtcgagagt gtaaaatatt ttgtgtaaat gaaaaaatcc
    21841 atacaaaaaa ggaagtc
//
LOCUS       DAFCHQ010000050        20664 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000050 DAFCHQ010000000
VERSION     DAFCHQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.050"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            168..362
                     /locus_tag="LNC71_001828"
     CDS             168..362
                     /locus_tag="LNC71_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466898.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="LNC71_001829"
     CDS             631..930
                     /locus_tag="LNC71_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466899.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="LNC71_001830"
     CDS             complement(1027..1461)
                     /locus_tag="LNC71_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466900.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="LNC71_001831"
     CDS             complement(1693..2403)
                     /locus_tag="LNC71_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBK6466901.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="LNC71_001832"
     CDS             complement(2393..3253)
                     /locus_tag="LNC71_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBK6466902.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="LNC71_001833"
     CDS             complement(3258..3896)
                     /locus_tag="LNC71_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBK6466903.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="LNC71_001834"
     CDS             complement(3911..4210)
                     /locus_tag="LNC71_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6466904.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="LNC71_001835"
     CDS             complement(4253..5737)
                     /locus_tag="LNC71_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBK6466905.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="LNC71_001836"
     CDS             complement(5757..6218)
                     /locus_tag="LNC71_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6466906.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LNC71_001837"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="LNC71_001837"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBK6466907.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="LNC71_001838"
     CDS             7521..8291
                     /locus_tag="LNC71_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBK6466908.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="LNC71_001839"
     CDS             complement(8564..9391)
                     /locus_tag="LNC71_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6466909.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="LNC71_001840"
     CDS             9959..11194
                     /locus_tag="LNC71_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK6466910.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="LNC71_001841"
     CDS             complement(12193..12453)
                     /locus_tag="LNC71_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBK6466911.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="LNC71_001842"
     CDS             complement(12480..13781)
                     /locus_tag="LNC71_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6466912.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="LNC71_001843"
     CDS             complement(14073..14531)
                     /locus_tag="LNC71_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6466913.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="LNC71_001844"
     CDS             complement(14544..15875)
                     /locus_tag="LNC71_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBK6466914.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="LNC71_001845"
     CDS             complement(15886..17328)
                     /locus_tag="LNC71_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6466915.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="LNC71_001846"
     CDS             complement(17315..18079)
                     /locus_tag="LNC71_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBK6466916.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="LNC71_001847"
     CDS             complement(18183..19058)
                     /locus_tag="LNC71_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6466917.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="LNC71_001848"
     tRNA            complement(19313..19397)
                     /locus_tag="LNC71_001848"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="LNC71_001849"
     tRNA            complement(19415..19486)
                     /locus_tag="LNC71_001849"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="LNC71_001850"
     tRNA            complement(19496..19568)
                     /locus_tag="LNC71_001850"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="LNC71_001851"
     tRNA            complement(19630..19714)
                     /locus_tag="LNC71_001851"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="LNC71_001852"
     tRNA            complement(19735..19805)
                     /locus_tag="LNC71_001852"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="LNC71_001853"
     tRNA            complement(19851..19922)
                     /locus_tag="LNC71_001853"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="LNC71_001854"
     tRNA            complement(19934..20006)
                     /locus_tag="LNC71_001854"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="LNC71_001855"
     tRNA            complement(20009..20082)
                     /locus_tag="LNC71_001855"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="LNC71_001856"
     tRNA            complement(20089..20169)
                     /locus_tag="LNC71_001856"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="LNC71_001857"
     tRNA            complement(20171..20246)
                     /locus_tag="LNC71_001857"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="LNC71_001858"
     tRNA            complement(20282..20354)
                     /locus_tag="LNC71_001858"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="LNC71_001859"
     tRNA            complement(20364..20436)
                     /locus_tag="LNC71_001859"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="LNC71_001860"
     tRNA            complement(20452..20523)
                     /locus_tag="LNC71_001860"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="LNC71_001861"
     tRNA            complement(20538..20627)
                     /locus_tag="LNC71_001861"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
BASE COUNT         6532 a         4320 c         3273 g         6539 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaagc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaaaggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgag
//
LOCUS       DAFCHQ010000051        20363 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000051 DAFCHQ010000000
VERSION     DAFCHQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.051"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            292..1431
                     /locus_tag="LNC71_001862"
     CDS             292..1431
                     /locus_tag="LNC71_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBK6466918.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="LNC71_001863"
     CDS             complement(1498..2073)
                     /locus_tag="LNC71_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBK6466919.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="LNC71_001864"
     CDS             complement(2078..3052)
                     /locus_tag="LNC71_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBK6466920.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="LNC71_001865"
     CDS             3335..4303
                     /locus_tag="LNC71_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6466921.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="LNC71_001866"
     CDS             4416..4694
                     /locus_tag="LNC71_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBK6466922.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="LNC71_001867"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="LNC71_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBK6466923.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="LNC71_001868"
     CDS             5151..5906
                     /locus_tag="LNC71_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6466924.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="LNC71_001869"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="LNC71_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBK6466925.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="LNC71_001870"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="LNC71_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBK6466926.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="LNC71_001871"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="LNC71_001871"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBK6466927.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="LNC71_001872"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="LNC71_001872"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBK6466928.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="LNC71_001873"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="LNC71_001873"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBK6466929.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="LNC71_001874"
     CDS             10643..11185
                     /locus_tag="LNC71_001874"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBK6466930.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="LNC71_001875"
     CDS             11208..12764
                     /locus_tag="LNC71_001875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBK6466931.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="LNC71_001876"
     CDS             12778..13680
                     /locus_tag="LNC71_001876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBK6466932.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="LNC71_001877"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="LNC71_001877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBK6466933.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="LNC71_001878"
     CDS             15123..15545
                     /locus_tag="LNC71_001878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBK6466934.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="LNC71_001879"
     CDS             16014..16244
                     /locus_tag="LNC71_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBK6466935.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="LNC71_001880"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="LNC71_001880"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBK6466936.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="LNC71_001881"
     CDS             17655..17831
                     /locus_tag="LNC71_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBK6466937.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="LNC71_001882"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="LNC71_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBK6466938.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="LNC71_001883"
     CDS             18326..18709
                     /locus_tag="LNC71_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466939.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="LNC71_001884"
     CDS             18878..19762
                     /locus_tag="LNC71_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6466940.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="LNC71_001885"
     CDS             19876..20112
                     /locus_tag="LNC71_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBK6466941.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="LNC71_001886"
     tRNA            20200..20280
                     /locus_tag="LNC71_001886"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="LNC71_001887"
     tRNA            20287..20358
                     /locus_tag="LNC71_001887"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3567 c         4425 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta tac
//
LOCUS       DAFCHQ010000052        19694 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000052 DAFCHQ010000000
VERSION     DAFCHQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.052"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..210)
                     /locus_tag="LNC71_001888"
     CDS             complement(<1..210)
                     /locus_tag="LNC71_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466942.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="LNC71_001889"
     CDS             complement(216..446)
                     /locus_tag="LNC71_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466943.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="LNC71_001890"
     CDS             1063..1725
                     /locus_tag="LNC71_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466944.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="LNC71_001891"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="LNC71_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBK6466945.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="LNC71_001892"
     CDS             5145..5459
                     /locus_tag="LNC71_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6466946.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="LNC71_001893"
     CDS             5472..5846
                     /locus_tag="LNC71_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBK6466947.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="LNC71_001894"
     CDS             5856..6200
                     /locus_tag="LNC71_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBK6466948.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="LNC71_001895"
     CDS             6271..7623
                     /locus_tag="LNC71_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBK6466949.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="LNC71_001896"
     CDS             7683..8825
                     /locus_tag="LNC71_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBK6466950.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="LNC71_001897"
     CDS             8850..9104
                     /locus_tag="LNC71_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466951.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="LNC71_001898"
     CDS             9360..10544
                     /locus_tag="LNC71_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBK6466952.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="LNC71_001899"
     CDS             10541..10678
                     /locus_tag="LNC71_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBK6466953.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="LNC71_001900"
     CDS             10683..10904
                     /locus_tag="LNC71_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466954.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="LNC71_001901"
     CDS             10917..11417
                     /locus_tag="LNC71_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBK6466955.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="LNC71_001902"
     CDS             11514..13361
                     /locus_tag="LNC71_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBK6466956.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="LNC71_001903"
     CDS             13364..14260
                     /locus_tag="LNC71_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6466957.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="LNC71_001904"
     CDS             14310..14699
                     /locus_tag="LNC71_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6466958.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16188
                     /locus_tag="LNC71_001905"
                     /pseudo
     CDS             14686..16188
                     /locus_tag="LNC71_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="LNC71_001906"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="LNC71_001906"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK6466959.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18893..>19694
                     /locus_tag="LNC71_001907"
     CDS             18893..>19694
                     /locus_tag="LNC71_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6466960.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6654 a         3219 c         4309 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acgcggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAFCHQ010000053        19648 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000053 DAFCHQ010000000
VERSION     DAFCHQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.053"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..669
                     /locus_tag="LNC71_001908"
     CDS             22..669
                     /locus_tag="LNC71_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBK6466961.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            753..1697
                     /locus_tag="LNC71_001909"
     CDS             753..1697
                     /locus_tag="LNC71_001909"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBK6466962.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1832..3283
                     /locus_tag="LNC71_001910"
     CDS             1832..3283
                     /locus_tag="LNC71_001910"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBK6466963.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3304..4140
                     /locus_tag="LNC71_001911"
     CDS             3304..4140
                     /locus_tag="LNC71_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK6466964.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4140..4799
                     /locus_tag="LNC71_001912"
     CDS             4140..4799
                     /locus_tag="LNC71_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6466965.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4925..5614
                     /locus_tag="LNC71_001913"
     CDS             4925..5614
                     /locus_tag="LNC71_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466966.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5620..6576
                     /locus_tag="LNC71_001914"
     CDS             5620..6576
                     /locus_tag="LNC71_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBK6466967.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6620..7132
                     /locus_tag="LNC71_001915"
     CDS             6620..7132
                     /locus_tag="LNC71_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466968.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7388..7936)
                     /locus_tag="LNC71_001916"
     CDS             complement(7388..7936)
                     /locus_tag="LNC71_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6466969.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8025..9206)
                     /locus_tag="LNC71_001917"
     CDS             complement(8025..9206)
                     /locus_tag="LNC71_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBK6466970.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9442..10950)
                     /locus_tag="LNC71_001918"
     CDS             complement(9442..10950)
                     /locus_tag="LNC71_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBK6466971.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11109..11300
                     /locus_tag="LNC71_001919"
     CDS             11109..11300
                     /locus_tag="LNC71_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466972.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11503..12420
                     /locus_tag="LNC71_001920"
     CDS             11503..12420
                     /locus_tag="LNC71_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBK6466973.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12413..13486
                     /locus_tag="LNC71_001921"
     CDS             12413..13486
                     /locus_tag="LNC71_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466974.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13634..14152
                     /locus_tag="LNC71_001922"
     CDS             13634..14152
                     /locus_tag="LNC71_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466975.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14133..14525
                     /locus_tag="LNC71_001923"
     CDS             14133..14525
                     /locus_tag="LNC71_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBK6466976.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14601..15536
                     /locus_tag="LNC71_001924"
     CDS             14601..15536
                     /locus_tag="LNC71_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBK6466977.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15669..16448
                     /locus_tag="LNC71_001925"
     CDS             15669..16448
                     /locus_tag="LNC71_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBK6466978.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16441..16758
                     /locus_tag="LNC71_001926"
     CDS             16441..16758
                     /locus_tag="LNC71_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBK6466979.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17002..18612
                     /locus_tag="LNC71_001927"
     CDS             17002..18612
                     /locus_tag="LNC71_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6466980.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18769..19617
                     /locus_tag="LNC71_001928"
     CDS             18769..19617
                     /locus_tag="LNC71_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBK6466981.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6350 a         3514 c         3956 g         5828 t
ORIGIN      
        1 ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct
       61 ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc
      121 agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg
      181 tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca
      241 gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa
      301 ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa
      361 tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta
      421 gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg
      481 atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca
      541 gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc
      601 aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa
      661 gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta
      721 gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc
      781 atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat
      841 tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg
      901 aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta
      961 tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag
     1021 tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa
     1081 acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt
     1141 tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg
     1201 aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt
     1261 attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct
     1321 atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag
     1381 tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg
     1441 ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta
     1501 caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt
     1561 tacagattgg tacagagctt cataaagaag ggccggaaat tttttctaga atcataaaag
     1621 aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat
     1681 tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa
     1741 aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg
     1801 ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga
     1861 gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa
     1921 gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca
     1981 cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa
     2041 aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac
     2101 acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc
     2161 ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta
     2221 tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc
     2281 tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc
     2341 tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat
     2401 gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac
     2461 agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc
     2521 gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat
     2581 agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt
     2641 aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc
     2701 actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc
     2761 aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc
     2821 catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc
     2881 tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct
     2941 attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga
     3001 tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt
     3061 tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct
     3121 gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg
     3181 aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt
     3241 tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga
     3301 agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct
     3361 tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa
     3421 gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat
     3481 gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa
     3541 atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca
     3601 gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca
     3661 gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc
     3721 cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg
     3781 cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag
     3841 tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag
     3901 aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc
     3961 aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc
     4021 gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat
     4081 tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga
     4141 tgtactttcc cctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag
     4201 gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat ttggtagggt
     4261 tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt
     4321 atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta tatttcgggt
     4381 taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg
     4441 catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg
     4501 ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag
     4561 cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt
     4621 cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc
     4681 ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta
     4741 tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatccatcag tcaatctgaa
     4801 aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct
     4861 gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga
     4921 aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga
     4981 gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac
     5041 gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt
     5101 gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt
     5161 tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt
     5221 tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat
     5281 tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt
     5341 catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata
     5401 ttttccaaaa gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt
     5461 ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga
     5521 gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca
     5581 gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga
     5641 ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat
     5701 aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa
     5761 ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc
     5821 aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct
     5881 gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt
     5941 gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt
     6001 gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa
     6061 attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca
     6121 gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct
     6181 gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct
     6241 atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac
     6301 agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa
     6361 aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg
     6421 gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg
     6481 catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct taaatattat
     6541 tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga
     6601 atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt
     6661 ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca
     6721 aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta
     6781 tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcag gcgtggatcc
     6841 cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca
     6901 tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca
     6961 tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc
     7021 taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga
     7081 atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa
     7141 tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa
     7201 gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct
     7261 caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca
     7321 tcgagaggtc gtgacatagc ttttgtcacg atctctcggc ttatcctacg agtattattc
     7381 gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat
     7441 cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt
     7501 ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc
     7561 gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat
     7621 actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca
     7681 gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact
     7741 ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat
     7801 cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt
     7861 ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata
     7921 catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga
     7981 aaatatttcc aaagctttta aaagtaactt tgcttttttg tattttatac cagcaagcca
     8041 gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt
     8101 tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg
     8161 atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca
     8221 gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt
     8281 ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag
     8341 gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg
     8401 aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg
     8461 atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct
     8521 ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt
     8581 gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa
     8641 tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca
     8701 ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa
     8761 acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc
     8821 ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata
     8881 tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag
     8941 aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca
     9001 aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt
     9061 gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc
     9121 aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa
     9181 ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt
     9241 acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
     9301 aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa
     9361 cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca
     9421 acatttataa gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc
     9481 gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac
     9541 agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat
     9601 tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc
     9661 caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga
     9721 caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc
     9781 cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc
     9841 gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata
     9901 gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat
     9961 cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt
    10021 ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac
    10081 gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt
    10141 accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata
    10201 gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt
    10261 tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca
    10321 tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata
    10381 cagttggttc aagcaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac
    10441 gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt
    10501 ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct
    10561 ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac
    10621 actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc
    10681 ataagtaatc ccataatcac cacggatctt attacgttca atatctccag catagacgca
    10741 aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc
    10801 aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact aacttcccac caaattctaa
    10861 atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata
    10921 tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt
    10981 tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa
    11041 atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa
    11101 ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga
    11161 tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt
    11221 atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa
    11281 gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc
    11341 agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt
    11401 atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt
    11461 attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca
    11521 aatgcaaatt atgagccaga cgaactagag gagacgaacg cggatcagga tacaggacaa
    11581 gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta
    11641 gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt
    11701 ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc
    11761 gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa
    11821 caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat
    11881 cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat
    11941 gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag
    12001 ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa
    12061 gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa
    12121 atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta
    12181 ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag
    12241 caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc
    12301 agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat
    12361 tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga
    12421 acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa
    12481 cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct
    12541 agaggaaaca aaaatacatg aaaaagaaaa agtaactccg acgaatacga atactgcaga
    12601 aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga
    12661 tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat
    12721 gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat
    12781 attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta
    12841 tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac
    12901 atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt
    12961 ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa
    13021 cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg
    13081 acactggtca gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt
    13141 gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga
    13201 caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt
    13261 ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga
    13321 aactacttat caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa
    13381 agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt
    13441 tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga
    13501 ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa
    13561 ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg
    13621 gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg
    13681 tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt
    13741 tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat
    13801 tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta
    13861 tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct
    13921 atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt
    13981 ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc
    14041 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
    14101 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
    14161 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
    14221 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
    14281 gtcaaaactt ttcggaaaaa gaagtggtct tcaaaaaagg cgatgattta ctgagtattt
    14341 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
    14401 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
    14461 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
    14521 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
    14581 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
    14641 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
    14701 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
    14761 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
    14821 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
    14881 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
    14941 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
    15001 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
    15061 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
    15121 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
    15181 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
    15241 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
    15301 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
    15361 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
    15421 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga
    15481 agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg
    15541 tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc
    15601 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
    15661 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
    15721 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
    15781 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
    15841 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
    15901 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
    15961 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
    16021 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
    16081 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
    16141 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
    16201 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
    16261 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
    16321 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
    16381 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
    16441 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
    16501 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
    16561 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
    16621 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
    16681 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
    16741 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
    16801 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
    16861 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
    16921 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
    16981 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
    17041 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
    17101 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
    17161 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
    17221 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
    17281 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
    17341 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
    17401 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
    17461 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
    17521 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
    17581 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
    17641 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
    17701 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
    17761 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
    17821 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
    17881 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
    17941 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
    18001 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
    18061 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
    18121 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
    18181 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
    18241 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
    18301 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
    18361 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
    18421 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
    18481 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
    18541 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
    18601 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
    18661 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
    18721 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
    18781 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
    18841 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
    18901 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
    18961 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
    19021 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
    19081 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
    19141 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
    19201 cttgcacata tcgatcgaat ccgagataat acctatgctt atctgattgt agatgatttg
    19261 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
    19321 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
    19381 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
    19441 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
    19501 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
    19561 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
    19621 cttttattca aaaaacatac caaaaata
//
LOCUS       DAFCHQ010000054        19234 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000054 DAFCHQ010000000
VERSION     DAFCHQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.054"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            174..1601
                     /locus_tag="LNC71_001929"
     CDS             174..1601
                     /locus_tag="LNC71_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6466982.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1693..2058)
                     /locus_tag="LNC71_001930"
     CDS             complement(1693..2058)
                     /locus_tag="LNC71_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK6466983.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2055..2420)
                     /locus_tag="LNC71_001931"
     CDS             complement(2055..2420)
                     /locus_tag="LNC71_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBK6466984.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2612..2860)
                     /locus_tag="LNC71_001932"
     CDS             complement(2612..2860)
                     /locus_tag="LNC71_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466985.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2859..2987
                     /locus_tag="LNC71_001933"
     CDS             2859..2987
                     /locus_tag="LNC71_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBK6466986.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3044..4738
                     /locus_tag="LNC71_001934"
     CDS             3044..4738
                     /locus_tag="LNC71_001934"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBK6466987.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4742..4861
                     /locus_tag="LNC71_001935"
                     /pseudo
     CDS             4742..4861
                     /locus_tag="LNC71_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4838..5011
                     /locus_tag="LNC71_001936"
     CDS             4838..5011
                     /locus_tag="LNC71_001936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6466988.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5389..6522
                     /locus_tag="LNC71_001937"
     CDS             5389..6522
                     /locus_tag="LNC71_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBK6466989.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6553..6873
                     /locus_tag="LNC71_001938"
     CDS             6553..6873
                     /locus_tag="LNC71_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBK6466990.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6902..7366)
                     /locus_tag="LNC71_001939"
     CDS             complement(6902..7366)
                     /locus_tag="LNC71_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6466991.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7498..8751)
                     /locus_tag="LNC71_001940"
     CDS             complement(7498..8751)
                     /locus_tag="LNC71_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6466992.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8780..9097)
                     /locus_tag="LNC71_001941"
     CDS             complement(8780..9097)
                     /locus_tag="LNC71_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBK6466993.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9123..9443)
                     /locus_tag="LNC71_001942"
     CDS             complement(9123..9443)
                     /locus_tag="LNC71_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6466994.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9907..11322)
                     /locus_tag="LNC71_001943"
     CDS             complement(9907..11322)
                     /locus_tag="LNC71_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6466995.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11339..12655)
                     /locus_tag="LNC71_001944"
     CDS             complement(11339..12655)
                     /locus_tag="LNC71_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6466996.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12805..13503)
                     /locus_tag="LNC71_001945"
     CDS             complement(12805..13503)
                     /locus_tag="LNC71_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBK6466997.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13792..14748)
                     /locus_tag="LNC71_001946"
     CDS             complement(13792..14748)
                     /locus_tag="LNC71_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBK6466998.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14861..15694)
                     /gene="msrA"
                     /locus_tag="LNC71_001947"
     CDS             complement(14861..15694)
                     /gene="msrA"
                     /locus_tag="LNC71_001947"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBK6466999.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15869..17503)
                     /locus_tag="LNC71_001948"
     CDS             complement(15869..17503)
                     /locus_tag="LNC71_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467000.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17490..19178)
                     /locus_tag="LNC71_001949"
     CDS             complement(17490..19178)
                     /locus_tag="LNC71_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467001.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         6109 a         3760 c         3192 g         6173 t
ORIGIN      
        1 aattcgcaat ttatttttta atagaattat ctgttttcca aattttataa cataaaaaac
       61 cttttttaac ggcatttata tgctttttca gaaattaact gatgatttca aaaagaaaac
      121 atgtcataat atttcccata ttgtgcaaaa aaatacaagg cggaggctct actatgattg
      181 aagaatatat tgagaaaaat attttaagac agctgttttt atgtgggcaa ttctatgtaa
      241 ataaagaagt caatctagaa aagctctcca atctcctgca tgtttgcaaa accactttac
      301 tcaatgatat caataacata aaaaaagaat ttgaagaaca gatcgcttat acacatcggg
      361 aaaaggaccg ttacacttta tatttttcag aacatatacc tcgatgcaag atcatgcagc
      421 aattgtctca aaattcgttg tttttaaaaa cttgtctgct ttatttggaa gaagatgaac
      481 ctgattatct tcaattaact gaatgtgaat tcatctctgt cagcaaagct tattcattaa
      541 aaaaacaagt attagcttat tttaatgact gcgggatcga aatcgaccgc tactcccctc
      601 gattcactga aatggaaaga cgtctgctgc ttcttaatgt gtcgtatcgt ttaggcggtt
      661 ttaattcttg ggagctacct gaatcgtttt ttgaacgtgc cgatcgtttc attgaatccg
      721 taacggaaaa tagtggcaga ttttatgata aagaaaataa agaaatctta tcgattggtt
      781 ttgctatcag tttcctacgc cagcaagtgt gtgctgtgac aatagattca aaatttatcg
      841 aagaaatcaa aaaacgtcct atttacaatt atgtcgaatc agcatgggaa aacactgatt
      901 tccaaaccta ttataaaaaa gaagaattcg cctttatttt gacactattc aatctttgta
      961 attatggttt ccattcttat cagttgattg cggaagactt tcaacagcta caccaagtgt
     1021 ttattgacaa tactcccgaa ataaaagaac tagttgctac ctttgaatct cacttcaatc
     1081 aagaattatt cggaaaccaa ccgttcgaac gtgcgctgat tcatctgatg cgttcggctt
     1141 gggataacta tcaattattc atgccagaaa aattttatct tttaaatgag gaacagacaa
     1201 atttatataa agaagtccag acaatttttt ccagctggtc cagccagtta ccttatgatc
     1261 tacggttgaa tcctaattgc atgcgggctt ttgtgatcga attgagcgga attctccgtc
     1321 tgacaaaaga acatctgact atctatatcg tcacaaattc ggatgttcat tatctgattt
     1381 atcgggaagc cttggaagct gtaactactt tcgattttca agttgcacca acaatttatt
     1441 caagtatatc tgatataaag aagtatgcgc agcagtcctc aaaccgggta ttatgtgaac
     1501 gtacattata tacaccagat gcggtccaat atgaaaatat tattcctatt tcgatcaata
     1561 cgatcgatcg agctatcatt tcagctgtgc aaaacaaata aggggctgtg acaaaaatca
     1621 tgtcacagcc tcttatttgt tgaagatgcc catttctgta acaatctctt gatacacgtc
     1681 aggctcacaa cctcacttat tttggacatc tacgggtccg atctctttat ttaaaaaata
     1741 agaaatgatg gttcggataa ctaccaacgc cgctagttgg aaaatatcct gcaaggtagg
     1801 tttgataatc gattcgatga tcccagctga aacaagaatt tccaaactta gaagtatata
     1861 gcctcccaac atctttttaa tagcattgtt ctcgctgact cggtcgcttt gcacttttgt
     1921 atggacaaat aaatatttca aaaaatcctt gattgataga caaacacccc aaaccaataa
     1981 acagatagca aagatatcaa gtcccagtac aagtttttcg aaatatggaa gaagaaactg
     2041 catcaactct gtcgtcatcc tacctctcct cttctttgtt tttctttgtc ttctatttcc
     2101 tgattcaaga aataagaaat gactgttcga atgataataa taagtgcaag cttcaaaata
     2161 tctgtccaag tcggcttgat aactgactcg atgatatcag caacgactag tacttccaag
     2221 cttaacaaaa tataacttgc tagcagtttt cttgtatctg cattacgagt attacgttct
     2281 gcatagtttt tcttgacatg agaccaatca aaaagtttga atagaacaag aacgatcccg
     2341 acaattaaga taccgataga acaaatattt aaaatagcaa tgatccattc tagaaattca
     2401 attaaatttg agcgaaacat atacgctacc tactttcttt taatttgttc ctattctttt
     2461 attgtaaaag aaagttacct tgttcgtcta tttaaaagtt gttctaatga taacggttcg
     2521 cttaaatttt tctttttcac tctcccattt ttctccctcc atccataaat cgtggaagta
     2581 tccaaaacaa cgtagcaagt taaaataagg cttagtccgt catcagcttt tcaaagaagc
     2641 actgtctcgg tgcttttttt gtacccaaat ttctaaaaat agaaccatac acctcctcaa
     2701 atcttttctt cttacctacc ctatttttat ttacaggaaa atcagacact tcttcttttt
     2761 tgtcaaaatt tcctttttct tctcgtccta tcatttggca ttctaataaa aacgaactat
     2821 ccttggatat agaaagagaa aacaaggagg cgtttttcat gagtaaaaaa ttaatcaaag
     2881 tcggcatcgg tttaggtttg cttgcccttg gcgctgcata tctaggaaaa aagacaggac
     2941 tattcgaaga tgacagtcat ttatatgatg aattcgaatc aatctagcat cctatttagt
     3001 tgaatcacta cggacatact tggaagacat tggaggaatc acaatgagaa aaagtaaagc
     3061 aataatgatt ggtgccggta ttgccaatat ggcagcggct gtttatttga ttcaagaagg
     3121 caaatggaaa ggggatcaga tcacctttta ttcattggat gatcacggtt caaatgatgg
     3181 tgcaccgacc gacactgtag tggatgaata ttggaataaa aaccatccat tagaaaacca
     3241 aaaaggctat attgctcgag gcggtcgcat gcttaattac cgtacctatg tagatttgat
     3301 ggatctgtta agtcgaatac cttctgttac tgagccaggg atgactgctg aagaagatac
     3361 gagagatttt gatgcaaaac accgaacctt tgacaaagca cgtttgatgg aaggtggcaa
     3421 aggaatcatt gatggcggaa aactgggatt caataataaa gaccgtattc ttctgactaa
     3481 actgattgct atgcctgatt cagaagaaga aaaactagac aatatcacta tcgaagaata
     3541 tttcaaagat gatccacact tttttgaaac aaacttctgg tttatgtggg aaacaacatt
     3601 tgctttcaga acacgtagtt ctgctcaaga attgcgtcgc tatatgcatc aaatgattta
     3661 tgaattcact caaatcgaac atctagtggg cgtcaatcga acacgttaca atcaatacga
     3721 aagtattatg cttcctttga tcgagtactt gaaagaacaa ggatgcaaaa tcattttgaa
     3781 ccgccgtgtc actgactggg aattcaaaga aacaccgatg caagatgaaa tcactgtgac
     3841 tggtttgaaa attaccaata cactgactca agaagaagaa tacgttgtgg tcgatgaaga
     3901 tacagcagtt attttcacta atgggtccat tacagattca gctactttag gagattacga
     3961 aacaccagct gtagaaaata tggattatgg tgcagcttct agtctctgga aaaaagccag
     4021 tgaacgtttc tacaacttgg gaaatcctga caaattcttt gctgatcgag atgcaagcga
     4081 atgggtcagc tttaccctga cgacgaaaaa ccatttgttg ttgaatgaaa tcacccgtat
     4141 cacgactcaa gaaccaggaa atgccttgaa ttcatttatt tcaaccacac cgatcacgcc
     4201 gttaggccaa aaagatgtca atatgtctat tgtcgtgcat catcaaccgc attttacgtc
     4261 tcagaaacca aatgaaacag ttatttgggg ttatttcctt tatccaagac gacgtggcga
     4321 atttgtggat aaacagtata ttaaaatgaa tggaaaagaa atgctagaag aactgattgg
     4381 acaactttca aaagtagatc ctggaccagt gaatattcgt gaaaaagaaa cagaaatttt
     4441 cgacagcgta ataaataata tccctgtcta tatgccttat gcttccgcgt tgttcaataa
     4501 tcgtgccaaa agcgatcgac caaaagtcat tcctaaacat tcgaccaatc tggcctttac
     4561 aggagaattt gtcgagcagc cttatcagat gatctttact gaacaaagtg ctgtacgttc
     4621 tggagaaatc gctgctttcc attttgcggg tattcctatg tcccgtcttg tcaaaacacc
     4681 gcgttacgac aaagatattc ctactctagc tcgtgccgca aaaaaaatgt ttgaataatc
     4741 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acaaccaggc taaaacagct
     4801 tcttatgttt ttgatttggt tggacttatt accgcagatg caagcttttg aacaccgttt
     4861 accaagaggt tatgaaaaag ctgccctgca tcaagtaata agagcagcca agctaaaaca
     4921 gcttcttatg tttttgactt ggttagactt attaccgcag atgcaagctt ttgaacaccg
     4981 tttaccaaga ggttatgaaa aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa
     5041 atggttcctc acgtttttgc ataaatttga cttaataccg agaaactgat cccttctgtc
     5101 ataacctctt tttatttatg tctgtttctt tttgaaaaca actatttttt caacgtttac
     5161 tctcttgcca atccaacatc atctaacgga tacaactatt tcaatgatta ccattacact
     5221 aaaaaatcgg aaacacacct ctccgaacac aaatacataa gactatagaa tgtttcattt
     5281 aagaaaaaaa gcgtttcatt atcaaaaagg taagatctcc tttaaaatga aattatctgt
     5341 agtactaata atgcagacaa ttttatgaat ctgaaaggag catctccaat gaaagcagtc
     5401 acttggcaag gaaaacagaa aatggaaatc agaaacgtag atgatccaaa aatattagaa
     5461 ccaacagatg caatcatccg tatcacagca acagccattt gcggctccga tctgcatctt
     5521 taccatcatg gtgaagcagt tttggaaaaa gggtatgttg taggacacga accaatggga
     5581 atcgttgaag aagtgggatc tcaagttcaa acattgaaaa aagggcaacg tgtggttatc
     5641 ccttttaata ttagttgtgg tcattgtcat ttctgcctga atcacatgga aagccaatgt
     5701 gatgaatcaa atccagatca gttatatggc gggcttttcg gatttggcaa gttgaacggt
     5761 aatcattggg gtggtcaagc ggaatatcta agagttcctt atgcagactc tacttcattt
     5821 gttgtaccag ataatgatct tcctgatgaa aaagttttat ttctatcaga tattttgccc
     5881 acagcttatt ggagcgttga aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta
     5941 ggttctggtc cagtcggtct attcgctcaa aaattcgctg ttatggctgg agcagagcgc
     6001 gtaatcgcag tagatccagt acagcaccgt ctagataaag cggctaatta caatggggtt
     6061 gaaacttatt tcttagaaga cacggcacaa gctggaacag aattgtatga actggctaaa
     6121 ggtggcgcag atgtcattat cgattgtgta ggaatggacg gattagagcc tgtcaaagaa
     6181 aaagcgaaaa atttagtaag cttacaaagc ggcacaatct ctccattaca gatggcatcg
     6241 caagctgtca aaaaatttgg aacagtccaa gtaactggtg tttatatgac tccagcttct
     6301 tcttacccgc tgcaagaatt ttttatgcgg gatatcgctg tcaagcatgg acaggcacca
     6361 attatccatc taatgccaaa aatttacgca atgattgcag aaggactatt cgatcctaca
     6421 caaatcatta ctcattcgct gccactatca gaagcagcac aagcatatga gatctttgac
     6481 aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt gaattcagta aaaaatacag
     6541 gtgattttct ttatgcccaa aatcaaacga aatacccatg agatcgaaat aggaaaagaa
     6601 gaagatatcg aaatacgagg acgtcgcttc cgcttctatt tccaaaatcg ttacacattg
     6661 atctcactgg ctgtcgattt attaaccggg attttctata tttttggaag ccttgcttcc
     6721 ttaactaaaa tccccgatcg atatggcacc tatttttatt tggctggtgc tattttcttg
     6781 acgattcgtc ctattctcag aatcgtaagg aacattttta tttacgacga aaaaaaagca
     6841 atgaagagaa caagcaaaaa aaaagaggca tgagaatttt tcaaatctca tgcctctttt
     6901 ttcaccctat cgtatcactt tgatacagac gagcggtcgt cccactttct tccttgacca
     6961 ttgtcaaaaa tgtccactcg tatcgttcat aaaacgtggt atgatctgta attaagtaaa
     7021 gtttttcgta cccaagttga tgcatttctt ttctgatttc cgctaatatc cgcttggcta
     7081 tcccctgctt acgcttttcc ggttcaacaa acagggcaca aagatttggt gtcagatcag
     7141 ggcggttatg gaagtcgttt tcaatgacgc ccgctcctcc gacgatggtt tctttttcat
     7201 ctaagaccac ataccattga ggaatacttt ccttattagc gattccagta cgaatacttt
     7261 cctcatacac aacagcagga actccccatt ttgatgaaaa ccaattcgcg ctttgtttta
     7321 ctaattctgg gtgttctctt aatttgatcc agttatatgt aaccaatgtt taaacctccg
     7381 actaaaataa aacgtttaca aaaattatac aagtctttat gaaaattagc aatgacaaca
     7441 atagtgatgt aaaaaaaaaa acggaaaaca atcacttgtt ctccgttttt tgattaatca
     7501 agcatctaaa gcttcttttc ttctttcttg gatcgtagct accttgacga acggtagata
     7561 gatcaaaata gaaagaacca aattgacagc ggctagtaca ccacctgcaa tactttttgt
     7621 tgccaataca ccgccaataa ttggtggagt tacccatgga ggcataacgg tagctgctgg
     7681 cactaaacct gtagaagtcg caaagtaagc aacactaact aagaccattg gtgtcaaaat
     7741 aaatggaatg aacatgatcg ggttcaacac gataggtaac ccaaacatcg ttggttcatt
     7801 aatattgaag acacctggcg caatcgacaa gtttgttaca accatatatg gtttgttttt
     7861 acgtcctact aaataaatag ctaacagtaa acctaaagtt gcacctgttc ctcctaagtt
     7921 gacaaagctg tcaaagaaag gtttgttcac gataaacggt gcgctatgtc cagctgcgat
     7981 tgcattaacg tttgcttcaa tggctggcgc attgattgtt tgcatcaaag gatcgaccat
     8041 attcgctccg tgtaaaccaa agaaccataa gaatggtgtg ataaatgcaa gcagtaaggc
     8101 agatggataa gagtttgcaa gtcccataaa tggttcttgg accactgtat agaaagaacc
     8161 tacgatatca tcaacaccga atgcagcaaa aattgcagca actaaagcaa acgcagagat
     8221 agtgatcatt gctggtagta atgaagcaaa tgcttttgca actgccggtg gcacaccatc
     8281 aggcattttg atgatcaatt tttcatttcc gcttaaacgt acaaagattt ctgttgaaat
     8341 aatcccaaca ataatagcaa taaacaaacc gtttgagctc atacctaatg ctccgcctaa
     8401 agcgaagaaa gagcctaaag aaatcacacc tgcggaaaca ccgtcttttc ggtagccgtt
     8461 agctaaatga taagcaagca agaaagcaat caagacagaa agaatggcaa atgtaccgtt
     8521 ccaagcagca gccccgaatc ctttccatga ttcaccacca aaaattgagt tcatcaattc
     8581 ttggaacccg ggaatcggta agttattgat cattactgct aatgctccta agatcattaa
     8641 cggcatactc accataaatg agtcccgaat cgcaactaaa tgccgttgtg caccaatttt
     8701 tgatgctaca ggtataaaat gtttctccat aaatgcaaca aatttatcca taaatcttcc
     8761 ctcttttctt tactgtagat tacgcgttca atcgacgata cagatcaatg atctcttttg
     8821 ctaaatctgt aaaagctata gatgtcatca gatgatcttg actgtggacc gtcaaaagcg
     8881 tgacttcaac gtgatttcct tgtgcttctt gtgtcagcat ctctgtttga gagtgatgtg
     8941 cttggactaa agattcctct gcatctttga ttttttcatc tgctaaagtg aaatcacctt
     9001 ttttcgcagc agcaatcgct tccattgcat cgctttttgc atttccgcca aacatgatca
     9061 gacccatgat ggcttctaga tttttttgat cttccattta ttgtatcctc cgataatttt
     9121 tattatttgt ttattagttt ttctgcttgg tcgagtactt tttcaccatt catcatgcca
     9181 tagtcagcca tattgattac atctaaagga atgcctttag gtgctagctt ttcagcaaat
     9241 tgattttgca taaagcgtac ttgcggtcct aaaagtaaaa cgtctacatt tttagattct
     9301 agttgattat ccgcatctga agcagataca gcaaagatgt ctgcttccat tccttttgct
     9361 tctgctgcct tttgcatttt tgtgactagg agacttgtac tcattccagc tgaacataca
     9421 agcatgattg ttttcttttc cattttaaac acgttccttt ctatacttac acttgtatat
     9481 aagcaagtag cgtgccaact ttaaaagcgc tttaaaaagt tgatatcatc acatttaagg
     9541 tctataccaa gttgcataca cactaaaaaa gaaagcgcac actaaacaca caaattaatt
     9601 tttattacct tttttctttt aacgtgcaca aaaaaagttg tgacacaaaa cttgtcacaa
     9661 cttttttcaa ataaacggta ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt
     9721 aaaaacataa gatgttgttt ttactttgct attcttatta cttgatgcag gacagctttt
     9781 tcacaacctc tcaataaacg gtgttcaatc agctgaccct gggtattagc tcaaaacgct
     9841 ggattcacaa cctcttttat ttcaaataac cagtaaatac aatgaacatc tacaaaaaac
     9901 ttctatttat ttatagattt cgtcaggcaa atccaattct ctcttatcaa tcaaagtctg
     9961 ataccacgaa gcagattttt tcaatgttct gcttcgttca tcgttcaatt cgattcgaac
    10021 taatccatat cgattcttaa atgcattcat tggtgacaca cagtcagtga atgcccataa
    10081 catatagcct tcgcaatttg cgccttcttc tgttgctttg atcaagtaat gcaaatgctg
    10141 tccgataaat tcgatacggt aatcatcttg cacgattcct ttctcatcca tatactgttc
    10201 ttcatattct cttcccatac cgttttctgt tatcagccaa gggatattat gataattttc
    10261 ttttagatac atggctatat catacatgat tttcggatag atttcccatc ccctggaatt
    10321 attcattttc ttcccaggta aatcaaagac ttcataatat cgttcaggat ggaaaggtgt
    10381 ctcttcattc cattgataac gaggggcgcg aacgcgttta ggataatatt gattgattcc
    10441 aaggaaatct actgtatgat gctgaatcgt ttctaaatcc tctgttgcag gatcgaaata
    10501 aatcgattgt ttttgacaca actccaggaa ctcttctgtg tagtatcctt ttaccatcgg
    10561 atcaaaaaat actcgattat aaaacaaatc atacatataa gcagcttttt ggtcttctgg
    10621 agaattggat cgagcataga ccatttcagg atttaaaata cagccaatcc ttccaccata
    10681 atttccgctg cggtaaatct ctactgcttt agccgttgct aaaactttat gataattcca
    10741 catcatccac ttttttgtgt tttgttcatg cggccagcga acagcatcca aatagcaacg
    10801 cgtttgggga acgattggtt cgttgaatgt aaaccagttt tttaccctat caccgtaacg
    10861 atcaaaggca atttgagcat acttagcata aaaatcaact acttttcttg aagaccagcc
    10921 atcatatttt tccaaaagaa aagctgggac ttcataatgt tccaaacaaa tcatcggttc
    10981 tacgtctgct tcgatcaaag agtcgatcat ttcacttata tgctgagcat attcttcatc
    11041 tactatacag ttctcataat cagtaaaaaa acgagcccaa ttgatagaag tccgataatg
    11101 tgtcagatgg atggatttca tcagatccac atcctgctgg tattgttcca taaaattagt
    11161 tgctacggct gggccatacc cattatgcca aacaaaatgt tcatttttat accagctatc
    11221 tataaacgaa tcctgtcctt ctttttttcc tttccaacct tccgtttgcc aagcagaaga
    11281 tgcagcacca agaataaaat tttttggaat agtaattttc atgatttcct cctgtttttt
    11341 agatcccatc attttccaca cgatttgcta cttttacaaa tggcagatag atcaaaatag
    11401 aaacaataat acaaacaagt tgcgtaataa ctgcaccgat atttcctgct gttgccagat
    11461 aagcattgac gatcggcggc attggccaag ggaccattac aactgctttc gccgcaaatc
    11521 ctaaagcggt tgcaaaataa ccaatcgttc cagtgataag aggcgttaag ataaacggaa
    11581 tagctaaaat tggattcagc atgatcggca taccaaaaat aactggttca ttgatgttga
    11641 agatcccagg agcaagggat aacttggtga tttgtttcat atcttcccgt tttccaacta
    11701 acaatactgc aatcagtaaa ccgatcgtga ctccagaccc gcccatactc atatacatat
    11761 cccaaaatgg catagtaata atgttcggta cttcttttcc agcttcaaaa gcttccgtat
    11821 ttacagcaat tgccgctaaa agcaacggtt cccgtatcgg cttcaccatt tgatttccat
    11881 ggataccaat cacccaaaag acttgcgcaa taaacatcaa tagcaaaata cccggtaacc
    11941 cttgtacgac accttctaat gggcgttgta cgacattgta aatgatatca taaaggtaca
    12001 ttcctgtaag ttctttgatg ataaatcccg cagccgcgat acccgataca gtcagaatcg
    12061 tcgggaataa agcagaaaaa ctagcagaaa cattcgttgg cacggtatca ggcattctca
    12121 gctgtagttt cttctgtttc ctcaaccagc aataaagttc aatagaaagg atggagacca
    12181 acattcccaa aaataaccct ttcgtatctg tatattgttt agccaatacg ttttctacta
    12241 taacagtctg attttcattc actaattcat aaacagttgg attaacagat aaataagaca
    12301 tcactgcgat gataccaggg aatacaccat ccactttatt cagcttggct aattcgatcc
    12361 ctatcagaaa aacagcatag atggtcaaga aacttagtgt gacatagctg atggcagatg
    12421 cgataggctt taattcttct aaaaaacgca agaaacttat ctgtgctaag ccattttccg
    12481 agtcaaagac cattgctgaa aacaatgtgc caaaagcacc cgtaataatt accggaatca
    12541 atgcagcaaa tgcgtttttt atcaccatga tataccttaa attattgatt ctcgttgcca
    12601 cgttccctaa aacattggct aatttatcca taaaactcat tccagatccc ctcattcctg
    12661 cttcttctcc ttgacgtcat ctctttttgt ctattagtca gcgcttacat ttcgatgaac
    12721 agaaactatt tactcctctt ttttaccaaa tataaaacaa aaattcatct gatgtattta
    12781 tcataaaata catcagatga actgtcaatt agtttctttt tctcctatta ttttctgtaa
    12841 ttctctccga tttattgcca tatggacagt cattgcatca aacgcagcaa gtgcattccg
    12901 cctttcgatg gcctgaacga tctctgtatg aatatcgatc gtctcgattt tcagatgctg
    12961 gatatcataa tttttattaa acaaagcaat tgattcatta atgattggga taatatgcag
    13021 caaggctacg tttccgctag cttctgcaat catcgtatga aaatcgatgt ctaattgaac
    13081 atgttcttgt gtgccttctc gaaaagaagt ttcaatcttt tgcgttaatt ttttcatttg
    13141 gacgatttga ttcttcgttg catattccgc tgccaaacct gccatcttag gctccaataa
    13201 atatctcagt tcaaataagt ctcgtatcat ccgatcctga tcttcaatca aagaaaaacc
    13261 gagtgggtca ttggcaatcc ctcgcttatc actgacaaat gtccccgctc cttgccgaac
    13321 ttcaaggata ttcctggaag ctagagcccg cacagcttct cgcaccgtgc ttcgccccac
    13381 ctctaaataa tcggacaatt catattcatt cggcagttta tctcctactt gcaactgcat
    13441 ttcctttaaa tatctaagga tcttgtcagt agttacttct actagggttt tattttcttc
    13501 caaaccacat acctcctttt catctgatgt attttttcgt aattattata acacgtttga
    13561 atcatctgat gattttttgt atacaaaatt gattaatccg ttcatagaca tacaaacgaa
    13621 gaagatattt accataaaaa agactaggat aaaattcact cctagccttc gatcattttt
    13681 attttgaacg acaaaaaata tgatgttcaa taacagacga tcttcattcg attcttgatg
    13741 tcatcttttg ttttttttaa tatatggata aaaagtttga ttacatcgtt tctattctag
    13801 aatttggtcg attctcaatt cttcttctgg tgagaaatct aaattatcca tcattcgaac
    13861 actttcttgt aactgactta accgacttgc tccgaccagt acacttgtaa cagatttttg
    13921 tcgcaaattc catgcaaggg ccatttgcgc taatgattgt cctcgtgata cggcgattgt
    13981 ttgcagtgct ttgatttttt ccagagttga tccaacttgt tcttctgaaa ggaatggtat
    14041 ttccttacga tgtgctctag aatcttcagg aataccatgt agataacgat ttgtcagcaa
    14101 cccttgagca agcggactga acgtgattgc ccctagccct tcttcttcta atactggaga
    14161 tagatcgtct tctagtagtg cacgagaaaa catattgtag cgcatctgat gaataatgaa
    14221 cggtgcttcc tttcttttta aaatttcagt catttttttt gtatcttctc cattatagtt
    14281 ggatatacca atatataatg cttttccttg tcttacaagt tgcatcaaag cttctgctgt
    14341 ttcttccaaa ggtgtatcag ggtctgggcg atgatgatag aagatgtcaa cataatctag
    14401 ccccattctt tggagacttt ggtcacaact tgaaatgatg gatttacgag agccccattc
    14461 cccatatggc ccttcccaca tatgataacc tgctttgcta gaaataatca gttcatcacg
    14521 gtaggcttga aaatcttctc taaaaatacg cccaaagttt ttctctgcag ctccagcagg
    14581 gcctccataa ttattcgcaa ggtcgaaatg ggtaattccc atgtcgaatg cgcctcttaa
    14641 aatctctctt tgattatgga taggatcata atcaccaaaa ttttgccata atccgagaga
    14701 taaaatcggt aatttcaaac ctgaatgacc ggatttacga tattccattt tttcataacg
    14761 tgcactatct gcaatataca atttttctcc tcctcatcaa atctttttaa aacgctttct
    14821 tttttctatt tttagtttac tacattttaa agaaaaggag ttaatcaaaa tgattttttc
    14881 gtatattttt ttgccatttg ttttttactc gttgatagat cagaaattga ttacgtgctt
    14941 gctggatttt tttataacgt ttcggttgtt ttttgtaaaa ttgctgatga tagttttctg
    15001 ctggccaaaa cgtaccggct ggttcgattt ttgtgacgat tggttggtgg aaagttcctg
    15061 aactgtctag tttctgtttt gattgttccg ctaattcttt ttgtcgttcg acttgataaa
    15121 aaatcactgg acgatattgc ttccctctat cttgaaattg tccaaatgta tctgtgggat
    15181 cagtaatttg ccaatagatt gtcagcaact cgctataact tatttctctt gtgtcataaa
    15241 tgatctccac agcttccaca tgacctgtgt aaccaccact tacttgatca taatttggtt
    15301 tttcaacatg tccgcccgtg tatcctgaca aaacagatag gatccctttt ttcgattcaa
    15361 acggctcgac catacaccag aaacatcccc ctgcaaatac tgcacgttct tgatagtcag
    15421 catcttgcat ggcatgcttg gaaaagtcat atttttgctc cttatcaaat ccttccgtaa
    15481 tcaataaata aaagtccatt acatcaggcg tcagattcat tcggatagct aatggacgta
    15541 gtgttgcttc tagttttgat aactgttctt taacggatat attttctctt atgtcattct
    15601 ttgtctgaat caatactttt cgttcccaat ctcgtgtatt cggattcaaa atcagattat
    15661 aaagtgtttg aatgaatatt tgttcctcat ccatagattc tcctcctttc cgaagaagac
    15721 aagctagtga ctttagctgt aatttatcat aaagtgagga aataagcgat tacttcatcc
    15781 ccctacagat ttttcgtata aaaaagagag aatccttatc gacagattct cctttttaat
    15841 caatttattt tataaatgaa aagttaattt accttttatg gaaaactatt ttaaaaaata
    15901 aacataaata tgcagttaat gtaacaagat aagaaataat agtcgttact gtataacttg
    15961 tgcttacttt aatatcagca actcctttga ctccgcttat ttgtaccaat ccattagcat
    16021 tcacctgctc ctttacaggt tgatgattta tttctacatt cgttcctaaa taagcatata
    16081 caggaagtgt gattacttgg ctcttctcat tagttactct atatatgata gaactaccag
    16141 ttgttttata aggaaggttt acccattgac tgccaatctt tgcttcgtgt tttattatat
    16201 gctccttatt ctccaatgaa gcgataggag aataatcata taaaaaataa tccattcctt
    16261 tagtggtata cgcctctact gtttctttgt ttacaacaat atgtcccggc tgagaaatta
    16321 ttttttgatt gaaatttttt ttactagaaa cagttaaagt gattaataac aaaacagtca
    16381 taaataaata aaatagttct cttgtcttat tgtatcttct atttttgaaa aatatcatcg
    16441 ttaatatcag agaagagaat attatttgca agcttaagat ccgccaagga aattgtaaca
    16501 atttggctgg cgtattttgc agaagatacc atggcaccaa actagttgta gctagccaag
    16561 tcaaacacgt accataataa atcttttttt ctatgtttgt taatttttta aataaaatca
    16621 ggggaataca taaagataac agaccaacta gtcccattga gtaagtagtt aagtcgttgt
    16681 tcatagcttc agtcagacta tcgaacacat tagatgcatg agcatataaa tttgtcacgc
    16741 taggtttgtt gattttttgg tagagcattt gttcaaacat cggataccaa aaagcactcg
    16801 tcagaaccaa tgtcattcct gccgcactaa ataatttttt aaatatagca ataaaatctg
    16861 cattcttgaa acctttagaa accaaacgaa taaaaataaa aatgataatt aatgctgtac
    16921 tcattaatgt tgatagtaca tggctgtaca ctagcaaact catactcaat gctagcttta
    16981 cagacggata cttttcttct tttccagaga tgatttgata agcataaaat aatacaggga
    17041 taacaagtgt tattgcgata gcttcggcaa ttgcggaacg atagtagatg tctactgtcc
    17101 gataagcaga gaaaatatag agacaactaa ataaaaatgc cgcttttttt tgttttaaaa
    17161 accgctcgcc ataatgataa gctacgagtg ttgtacacaa gttcaataac caaacgtaaa
    17221 taatatagct tacaatcaaa ttttttgtca tccaatataa cattacagct ggaaaaaaag
    17281 tcagaaaggg ataaaaataa tttacaccat atccagtata attgaatgtc tcataattaa
    17341 tcggactggt aaaaatagtc gataacccct tgattcgaga taagtgaaaa gaaagatctc
    17401 cagtggtata aatactctgt gtatttttga aaaagatgaa agaatatata ctactaacta
    17461 taattaatcc actcaatatt actacttttt tatttatttt cattcttctt gtttatctcc
    17521 tttttagtca catatcgaga atctttcaac ttattaggtg caaatcgttt aaaacttgaa
    17581 tacaccacta aacaataagt cagaatactg atccataaag ccatgagagc agttggagta
    17641 tattttgttg taatttctat cttattttct ccagcattta catccacatt cactaagcca
    17701 tgttttttat aagagtcatt tacgacttta ccgtttactt ttacctgtgt acctaaataa
    17761 gcatatactg gtactgtcac tttttgcttt ttgttgcttg ccactgtgta agtaatgtta
    17821 ttatctgcag ctttatactt ccctttttcc cacgattctc ctacttttac ttcatgtttt
    17881 tctaagtgag gcctttcttt tatcgcttct gttggggcgt aatcgtataa tcctcccata
    17941 tctgatgttg tataacgtgg gagtgtttct tcattgataa caatatgacc gtgttttgat
    18001 tggataactt ttgcgtaatt ttcttttgct gctatagacg tgacgagtag aaacaccaca
    18061 gtagcgccaa tgattaacca tgaatatttt ttattcatag tctttttcat aaaaacaagc
    18121 gttatgatca ttgaagaaaa aaagatttgg atacctaaga tacgccaagg aaactggagt
    18181 aagcttatcg gtgttttttg aaacaaaaac catggaaata aattagttac gcatacccat
    18241 gtagaaacta cgccaacata gatgattttt tcttttttct ccatttcttt cagtatgaat
    18301 aaaggaaaaa tcaacgcaac caatcctaat aaccccatag tgtatgttgt aatatcgtta
    18361 tttaacgctt gaatgatact atctgaaata gaaagtgctt ctagctgcaa atctgttttg
    18421 aagggactgt gtatttcttg ataattcatc tgctgcaaca taggatacca aaaatagcta
    18481 gttaatgcac aagtcaaaaa aacagccttt actaaattca cgaccacaaa ttttatatcg
    18541 ttttttattt gcttgcttaa aaacaaacga actataatca tacaagcaat caatgcacta
    18601 cacaatagtg tagttaagac atgagtatac acaagtagac ttatactgac agataaagcg
    18661 atgacttttt tttctctatt gaatatcaat ccataagcat aatataaaac agctgggata
    18721 atcgtaagag cgattgcttc tgcaattgcg gatcgataat agatatcaac tgttcgataa
    18781 gttgcaaaag tatataaaca actaaataaa aaagcagcat ctatcttttt aaaaaacttt
    18841 tctccataat aaaaactaat cagaatcgta caaatattta ataaccaaat atatagaaca
    18901 taacttaata ttaagttatt tgaaattcca taaaaaacga ctgcaggaat tattgtaaga
    18961 aaaggataaa aatagttcag tccatctcca taattgttaa atgttgtata gttgattggc
    19021 ccttcaaaaa tagaagataa ccctttgatt cgtgaaagat gaaaagataa atcacctgtt
    19081 gtataaattt cttgagcatt tctcataaaa tttactgaat ataaagtact aagtaagata
    19141 agaactacta aaagaaatgc ttttttactt attttcatag atttgttgtt ctcctttttt
    19201 aattctattt tcagtatact agaattgtct tttt
//
LOCUS       DAFCHQ010000055        18579 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000055 DAFCHQ010000000
VERSION     DAFCHQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.055"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..876)
                     /locus_tag="LNC71_001950"
     CDS             complement(<1..876)
                     /locus_tag="LNC71_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK6467002.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLST"
     gene            complement(1253..1891)
                     /locus_tag="LNC71_001951"
     CDS             complement(1253..1891)
                     /locus_tag="LNC71_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467003.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1895..2665)
                     /locus_tag="LNC71_001952"
     CDS             complement(1895..2665)
                     /locus_tag="LNC71_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBK6467004.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3083..3499)
                     /locus_tag="LNC71_001953"
     CDS             complement(3083..3499)
                     /locus_tag="LNC71_001953"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467005.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3550..3931)
                     /locus_tag="LNC71_001954"
                     /pseudo
     CDS             complement(3550..3931)
                     /locus_tag="LNC71_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4057..4722
                     /locus_tag="LNC71_001955"
     CDS             4057..4722
                     /locus_tag="LNC71_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467006.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5119..7152)
                     /gene="brxL"
                     /locus_tag="LNC71_001956"
     CDS             complement(5119..7152)
                     /gene="brxL"
                     /locus_tag="LNC71_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HBK6467007.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7270..9792)
                     /gene="pglZ"
                     /locus_tag="LNC71_001957"
     CDS             complement(7270..9792)
                     /gene="pglZ"
                     /locus_tag="LNC71_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HBK6467008.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9908..13540)
                     /gene="pglX"
                     /locus_tag="LNC71_001958"
     CDS             complement(9908..13540)
                     /gene="pglX"
                     /locus_tag="LNC71_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HBK6467009.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13604..17275)
                     /gene="brxC"
                     /locus_tag="LNC71_001959"
     CDS             complement(13604..17275)
                     /gene="brxC"
                     /locus_tag="LNC71_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HBK6467010.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17300..17875)
                     /locus_tag="LNC71_001960"
     CDS             complement(17300..17875)
                     /locus_tag="LNC71_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HBK6467011.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17875..18474)
                     /locus_tag="LNC71_001961"
     CDS             complement(17875..18474)
                     /locus_tag="LNC71_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HBK6467012.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5725 a         3553 c         2602 g         6699 t
ORIGIN      
        1 tgtactcaaa acattttcaa agtctttgtc tttgattttt acattagcgt ctttcaatac
       61 ttcgccaatt actttattct ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc
      121 tttcaattct tttttgtatt tatccatgtc gttgcctttg ttttggtttt tcaccatttt
      181 aaccacataa tattccgtag tataagttga agcattggta gaagtgatca catcagaaac
      241 ttgaccatct ttcaacttaa aggctgcttc tttgacttct gccggaaccg ttgtagtagt
      301 ggaatcaaat ttcacttttc cgccatcttc ttttgtcgta gtatcagttg acttgtcttt
      361 ggctaattta gagaaatcgt ctcccttatc agcagctttc ttcacatctt ttgcatcatc
      421 ttcacttgct acttggataa tttgagcttc cacttctgga tggaaagatt cccaagccgt
      481 ttttaaatct tcatcggtca atttgatatg tttctttaga ccttcttgaa aggctagact
      541 ttgtttgata ctgtctttga atgtttgttc tgtgtaacct gcagatttca attgtgaatc
      601 aaatgaatca cccaattgtt tctttgtttg atcatattgg gaatcgattt ctttggttga
      661 tacgtcatct ccatatttat cttcaaatac ttgataaacg atcaactgtt gcacagcgga
      721 atctgctcct tgcatttttt tcaagctatc cattacttct tgttcgctga ttgtaccgcc
      781 tttataacta acggctgttt tttcaccggc attcgtacag cctgacaata agccagtgcc
      841 tagtacgatg gcaagtgcta aaaatctttt tttcatttat caattctcct acttttcgtt
      901 ttttgcgaat tgctaaaaac aaaggtgttc aagatgaaaa tcatcaagaa cacctttact
      961 aatctgctcg atacgtcctg aatagatata aggctacaca atccagaaag ttcgctcagc
     1021 agaataaatg tcaagtagca catcacacct taatatacta cttcttttgt taccgttttt
     1081 caagtctatc ttgcgttccg tatttaacgt aactaattct tagaacaaat gttatcgatt
     1141 ttgtttttct tcttccgctg ttgggatatt caaatcagac acttttatgt tcgattttcc
     1201 agacattttt ttgttgtttt tattagaaaa agtttgtttt ttcatttgtt ttctaaagca
     1261 tgatcttctt gtttcctatt ttggtcagat atggatcatg cgtgaccacg ataactaacg
     1321 taccttggga agcctgttgc gataagattt caacgacttg ctccttattt cgtttatcta
     1381 atgatccagt tggttcatcg gctaaaatga ttttgctatt cttcaataag actctcgcac
     1441 aggcgattct ttgcttctca ccaccagata atgtgtagat aggcgtactt agcttcttgt
     1501 ttatatttac ttttttcaat gcctcatact ttgctgttcg cttatctgaa acatttaaat
     1561 tcgaaaactt caatccaata tctaaattaa aatcgattgt ttcatattca atcaacccaa
     1621 aatcttgaaa aagaaaagag agggcatttc tttttatcaa gcgcttctct cttccagtta
     1681 aatgttttgt ttcttgatta aataaataaa attgccctga actgaattga tcattcaatc
     1741 ctaatatatt caataaagta cttttccctg ctccgctctc tccaactaca gtcacaaaat
     1801 ccccagcatc cgcagtgaaa cgatccaaat gaagaatcgt tttgtttcga atgatttttt
     1861 tcgcaccttt tatcactatc aacggctcca attgttaacc tccattcatc agcaataact
     1921 gacttttctt ttctgtagac aaaataaaaa caactgaaag aataaaatca atgaagctaa
     1981 agaaaaaaat attaaaaaga tatttaggct ctctttctat aagaaaaagg aagattacac
     2041 ttgtcgttcc ccatttgatg aggttcatca tgaagaaatc tttatacgtt tcaaagagcg
     2101 aataaccact tgaacgaaag atagcgattt tccgacaatt gtagacaaaa tatatgcata
     2161 acatgacata gctcaacaag aagaaagaaa agaaagtgag taaaaatatc tgtatcaagt
     2221 aatgaaatga tctgcttaac actttgacag ctatctcttc agtctgagta tacggaaatg
     2281 ataactgcaa gttatcacgt aactgacttt cttcaatcag ctcaaataac ctttctttcg
     2341 acaaagagcc tatcttgatc tttaacggtg gatagatttc tccgctgaga atatttcttt
     2401 cccaatacgt actgtttttc aatgtcagta tttcaacaac cggataatgt tcgatccagg
     2461 ggatagaagg aataaatgta taaatgggtt gattgttttt tataaataga aatttcggtt
     2521 ctttttcact gatttcttga taatattctg ttatttgcgc caggtcattt tttaaatctg
     2581 tatgtctata tcgttcaggg atcaatataa cagggtgcgt ttctttttcg gttatttgga
     2641 tcaaccttcc agagacatca atcaaaggaa atttcttaag ataattcaga tttattttta
     2701 tccctctgcc aaactgttca ttttcttcat tcaagtaatc tgatacattg actaatatac
     2761 ttccgttttt attcaaagtt tcatataatt cctgatcggc tgctgatgca aaacgattgg
     2821 aataaataaa atccacgtgg tttttcccga tggttactgg ataaaataca tgatagtcct
     2881 caccagcgat ttcttttggt gtttcatggg aaacagctaa ctgtgaagaa gaaaaaacga
     2941 tagttgctaa ttcgacattg gcaacggtca aaagaagaga aaacaacaat ccaaatgtaa
     3001 aaagaatgta ttggatatag ttgaacgctt ctcttaacga agcgattttt tcaactaata
     3061 aaagagaaat caaagagata ccctatattc cgctgaacaa gaaaacgtac atctggaaca
     3121 aataaagaaa ttgctcctta taatacataa aaataaaacc accaatgatt cctgcaatta
     3181 tgaatgtaat aatgattttc tttttcccca ataaacacca caatacttga tgtaatgaaa
     3241 agccattatt caacaataaa ctgatttctt tattcatcaa taagaaaaag gatgataaca
     3301 aaaggaataa aagaatcaat ccaatagtta aatacactcg ataattatat acgtctgtac
     3361 ttgaaataac cgaatacgaa tagctatttt caaagtcttc aaaaatatta gtggcataat
     3421 cagtgtgaaa tgcttgattg aatcgttcca caaatagtaa aatgaaccgt tcataggctt
     3481 ctttttctct cgtcatcaag aaaaattcac cttcaaaatc ttttattttg atcgattctt
     3541 caatggacgt tacgcgaaag gaggtattag aatagggtac cttctcgtga gaagatttga
     3601 catccccttg ataatacaat gtcatttcaa gctttggctg tatcttgaag ttccaagatg
     3661 tatctacttc attggattca tttactgttt ttttcatata agaaagctca gtttctgatg
     3721 agacattttc aaataccatg attagctgct catattcttc ttgcccaata aaatgttgtg
     3781 gcaatcgaat cggagtataa gtatctgttt gatggaaaaa ttcatttgtc cgatagagta
     3841 cctgtttatt tataataacc aaggcaaaac cactcatgat accggtcact atgattgtga
     3901 gtaaaactgt gaactttttt ttgattatca ccactctccc ttacttaaat gattctttga
     3961 taaaatcata ccgtttttca tcaaaaaaac gtatctatca aatgtatagg tcatgtagaa
     4021 taaatctata tactgtagaa tcgaaaggag aagaagatga acatcggaga aaaaatacga
     4081 acttaccgca gaaattgtaa gcttacgcaa aaagaattag gcgaaaaaat cggtgtttcc
     4141 gataaaacaa tctctagttg ggaaaattct cgtacaatgc cagaccttga aatgctgtca
     4201 cttttacatc agactttagg tctgccgatt gattttcctt ctattgaatc agctaccgaa
     4261 tcaatagaac caacattgcc ggatgaacct cttgttatca agaaatctta ttcatcaaaa
     4321 caacccttct cactcaaaaa gacactatct ttctttctgc tcgtcttttg tttggtctat
     4381 ctttttctta tcaattatca atataaaaat gaaacggttg atcgattcaa tccttttttg
     4441 aaaccacaaa tcggctatac cacactccct gtaggatacg tggaaaattc catcgaacat
     4501 tgggtatgta atgatatctt tgttcatggt acatggctgt cttttcacgc tggagaggta
     4561 gaaaaaggta agaatcatgc tgtaatcaaa cacaaaggaa aacacgtcat gaacgttcgc
     4621 ctgctcactt gggaagccat tccctcagat taccaagaaa tcctcatcca agactatgac
     4681 gatgccttaa aaagaagccc tcaggagcca gataggcatt aagtaggaga tgaacgtaga
     4741 gatagaaaaa atcaatgcac atgatcttat caaaaaaaaa caggggtaca aattaattga
     4801 aaaagctttg agatgaaagt tcctaaataa aaaagttcag aatgataggt aaaaaaatga
     4861 ctaactattc tagtattagg ctatttgtat tattcttctg ttatttttat atcaaaaaat
     4921 ccagaaataa ctaaatatag gtatttctgg cttatagtat aatatttacg ccacagcccc
     4981 caatgattct ttttggaatc atgatcaagg acggacatac acaaaaatat tttgcccatt
     5041 cgatccgaat agattaacga ctaatctagt aagtaatata gattggtacg tagtcaaacc
     5101 agcaaatgga aaagttgtct attccacccc aagggcttta aagacagcat cttccggcgt
     5161 ttgataaaaa atcaaattaa aggtacccat taattctggg ggtacagttc caatatcagc
     5221 agcgcttatc ataggtacaa gtactttttt cgctccggca tcttttgcta cttgtaaaca
     5281 gttagctaaa ttatctacct taatcataga accaccaatt gtaatatccc ctaaaataac
     5341 taaacttggt aatacaggtt tatttaagga aactgagcat aaagctatta tagttgataa
     5401 cgataatgtt tttgtcatac caatgccatt catatcttga acatgaacga caaaatcttt
     5461 tgtcgtcaaa ctaatagact gactaacact tccataatta gctcttaaat atcggtatgc
     5521 cgaatccatc gcttctcttg ctccagcatc tgaattgatc ccagtacgtt caaactttcc
     5581 atttcccgaa gtcatttgcg tttccaattt ataggtacct aattgcccag ttttgccata
     5641 agataccgtg tacaaatgcc ctggcttccc taacccttct ggaatcaaag ttcctccacc
     5701 ttgttcagga acagaaacat actcttctcg ctcttcttct aaatcaatgt atgaaaaatt
     5761 gacatcataa aattccattc caccaatttt ctttaattgt tcttttacac gacgacgcat
     5821 ttcaagagca aatcttagta tttcttcgac atcacttttt tcaaatctac catcaggata
     5881 caacagtttc atcatcccag atacaatccg acgaactgca atcgtatccc gctgatttaa
     5941 ttgagaacca agtttgaaat atttatcaaa actatcgcca aaactaactt ttcgaagctc
     6001 acgcatgact tcagaaaaat aatctgtaat gaagccatag tcatttgtaa aaaaatctgg
     6061 tcgatattta ggaatttccc aacctggtaa ataacaatgc atccgatcca aaaaggctga
     6121 atccgtcccc ataacctccg gaaatggctc aaataaattc gaagttttca atattacatc
     6181 aacgctttga ttaatatttc ctacaaaagc cattgatgct gaagcattct tttcttcttt
     6241 gcctctagca aaagatcctg aagccatgta atctttcatg atttgaacgc catcattatc
     6301 cttaaattta atcccagcaa cttcgtcaaa agcaacacag tcccacatac ctacaagtcc
     6361 cattgtacga ctagccatat tatagaaaag gtttgctact gttgtttgtc cacctgaaac
     6421 aaggattgag tttggtgaaa tttctttata tacatgcgat ttacctgtac cacgtggtcc
     6481 tagttcacag agattaaaat tattttcaat taatggaata agtcttgcta aatgaagcca
     6541 ttttaccctt ttattaaatt gtgtaggttc cataccaatg gaccgtagaa gtaagtcaat
     6601 ccattcattt gtagtaaact ccttgcgagc catcttgtaa gcctcaatgt ctaaaccagg
     6661 catttggatt ggatcaacct tttttgcaat aaaagggcta ttccaacgat cattctcatc
     6721 ataccaatat tccatcgaaa caatacacca gatcccccca cctaataatc gatcaaattt
     6781 tttcggatac tcttcactaa tttgaacatt ttttaatccc aggttagaga attctgcttc
     6841 ataacaatct ttcttttcat ttaaatgtac agttacttta tcaataactg tataaccacc
     6901 caattcacga atttttgctt ttactttttc tgcttcatca ggacgcacat agtttcgagc
     6961 aagaatattt ttaacatttt ccacaccttt ttcaaccaat gtttcatcca atgacgaagc
     7021 atacatacca agtaaatatt ccaatacata aacaggtaca ttcgcacctt ctttaatttt
     7081 ctttgttaaa tcttttctga caatctttcc gggaaagaat tcagataact ttaaaatttc
     7141 ctgttgttcc actagaattc ctccatgtgt tcagtcatta taatagcaat taaagtatat
     7201 catacttaac cagtaggctt gataatacaa aagaaaaagt atccacaaaa aattgtggat
     7261 acctcgtttt tatatatcaa aatcaaatga ttctgcaaaa ggattatcta taatgaattt
     7321 tattcgctcg gtcaaatcat ttttttgatt ttttatcact aaataataag tatcttcaac
     7381 tgtatatcga cgatttacaa attcaaaaac aaatttagta aaccgctgat ttgcatcttc
     7441 tgtttttgaa tccgcaacat agatttcttc atttgagata cgttcaccat attcatcttc
     7501 aaaataaagc cagaaagatg cggggtcata aacatcacta attgcatttt tttgatagaa
     7561 ttctaacgtg gttgataatc ctgtaattct gcggttgcta gtcattaatt caattgctac
     7621 tggttccttt gcattgctac ctttggctac cgtaatttct agaacaggga ctagcatttc
     7681 ttgaatagat gaccctccat gaacgtagct ttgtcccccg ccagctttct taaaaatact
     7741 tgaagtaatc gggacacaaa tgtttctagt gtcatcattt cctaaagaat ctcccaaaga
     7801 gtaatttttc gtaccaatta tttcgtattc ttcttcacta agaataaaac gacgttctac
     7861 acgatctttt tcatttctag atggattttc aatcttatct acttcttcaa cgtggcttct
     7921 tttataaata aatccatgat cagccgtaac tataaaacga tagatactgc catcattatg
     7981 caagcgatta ataagtcggg aaatttcgct tatcgctaaa tcacaggcct caaaaactcg
     8041 attttcattc gagtgatcac ctgttctatc cacttgatcg tgataaatat aaatgactga
     8101 tttgccatta aatacttctc gtacctcagc cttattcatc ttaaccacat catcataatt
     8161 taaagctaca gcatttgcat ctcgttgctg gagaatcgca tttcgattgg ataaaccatt
     8221 cgtttttttc ccatcaatca gtacttctgt tccttcattg taagttagtt gattgtttgg
     8281 taataaggcg gctttaccga attcagtaac tgaaggtaat acagaaagaa agctagccat
     8341 gtttgttgaa tacttctttt ctctaactaa cttacgttct aaattcatcc ctaactcata
     8401 tcgaaaagca tctgaaataa taactacagt tttaacagag cgtttcatta cagcttgatt
     8461 ataaaatttt cgaatggatg gacgattctt aaattcaaaa gtttgattcc aaatttgtcc
     8521 tgattgatca agaaacttcg aataatttgc ttcaactaat cgatacaatt ggccatattt
     8581 ttgcttttct tctatatgat cactattcca aataaatttc cgataatgcg tatcaaccca
     8641 atacatttcc ttctcatatt gagttagcaa attatccatg ttcatcggca agcacaatct
     8701 gtttttatcc acaagataat aagcatgttt caataattga tactcataaa tataatccga
     8761 tgcgaaatgc attttttcgc gctgctttaa actatcaaaa attgtttgtc ctgcaatttt
     8821 cgttgtagtg tcagaagcct ttaatcgttc tgcataaaac attaatattt ttgcatgaat
     8881 ctcagaaaat acgtctgctt caattagatc atcaatttct atactgtcta ataatccatc
     8941 accatcaata gcctgatata cttttttaga tagctgttca aagctttcca tgtaacgtgt
     9001 atcattcata aactgatcca tgaaagtaat aatgtttgct ttcaaatgta gtatttcaaa
     9061 tcctttgaac ttcttcggca tctcttgatc aatttgataa taaaaatagt tcaaataaag
     9121 acacatcgtc agcttttcca atgttggctg gctatccgta aagccaaatt tcatttgaat
     9181 aaattcccaa aatttagctg ttaactgata cttatcaagt tcccttaaag gattctccct
     9241 actttctgta aagcttttta gaactacacg cagtaaatca tttaatcgaa tacttttcga
     9301 tttcaacaaa acagccatta tcattaattc aatcgtttct gatttattaa ttgtcatatg
     9361 cgtactaaat tcaccaaaac ttctcatgcg atcctttgca ttaaagaaat tacgatactg
     9421 tttaactgtc tgatgaaatc taagtggaat agccaattca ttcatgataa tcgctaagcg
     9481 gtctgcacta aattcctctg aataattcaa tacactcaac aaatgattat cttcatcttt
     9541 atcatcccaa cgaggaaatg gcgcatagat taaatagtta gtttcgggat cttcatattc
     9601 caataaacgc ttagttttaa attgtgcgtc cttttctaat cgatagattt ttgcattttg
     9661 aatgaccaat gagtcaattt cttcaagaaa atcctgattt gaatcaaacc aaaaaatgat
     9721 tttcctacat ccataattag gaaaacgttg atttaatttt tcttcaattt gttgcgtatt
     9781 tatatctgtc atcaatctca cctaatcgtt ctctttctat accccttata ttgacataat
     9841 ttggataatt tcgcacgcaa tttaagaata aaaaagaaag ttgttagaaa acaactttct
     9901 tagataacta tttttttggt tctttgaccc cttttgcaaa aatttggaat ttctcgccgg
     9961 ttttaggatc acgctgcaac ttatcataat tgacaattac accatcatct aagtctaatt
    10021 caattcgttt taatgctaag tgacccaact tctcgtcata ctcacgaact tcttttaatt
    10081 gcttctgaat ctttacaatt tcttttgtca ttgcatttct ttcttttggg ttatcacttg
    10141 ttgaaatttg ttgctgacgc aattcgattc gattgtcata cgctttttgt agttcgtgta
    10201 ggtattcagt acgtactttt cctaattgat ctggtgtata gcgatgcaag tacattaacg
    10261 ctttaaagcc attttgtttt cctgagtcca attgccaata aattggtcga ttggagtatg
    10321 tttttaaatg gtctttgtaa aaatcttgta cgaagtaacg acgaataatt tctcgacttg
    10381 tccctttgcc acctaaagtg tcagcgataa actggagatt ttcttcaagg gtttctttac
    10441 caaaagtaat ctcaacgaaa tcaatgaaac gattgacaat gtcaatttgg ctatccttga
    10501 aatactcatc atcggtgatg agtaaaacat tatcagtatc aggtttaaat ttttgatagc
    10561 gggaaggatc ccattcgccg ccagcgaaga tgagaccatc ttcgtctagg gagtaacgac
    10621 cgaagataca acctattgaa taagaaataa aatccttaat ttccctcgcc ttatcaggta
    10681 accttgcaga gatatattca tcttgaacct cttttgttaa ttctttttca agagaataat
    10741 gattaatgaa tatgtcgttc agttcctctt cattcgattt tagagtatta aattgattaa
    10801 ccaactgcct acttagacag ttaaatgcct ctgagagatc ttttttaaac atataactta
    10861 acaaaatgtt tcttctaaac tcgtatgacg tttcaagagc attatagtct tttctagcta
    10921 tatcgatatt ttcatccact ttttctctta ttttttttaa ttttctctca gatgtaactt
    10981 taagaaaagg tagttttccc attggtcctt catggtaatc caaggtggga gaaataaaag
    11041 ttaaaaagtg attaactaat ttagagttta catagccaaa tatttcttca aagtcagtta
    11101 tatcctttga gaacagaaca gatcctttag tttcaaaaat tgcctgattc tgcaatctaa
    11161 aagatacttg tccagatgaa agtgtcgacc aagtaattcc ctttttaaaa tagtaattaa
    11221 gattttgtgg tctagatttt aacttcccat tttcattaac ataatttttt atttcatctc
    11281 catcattctc ccagttaacc acgtattcat tgtttccata ccattttcta aaacgaccac
    11341 ctttagttgt aggaaaccat ttttttctag actttttagc gtcctctaat gttttagcat
    11401 caaactttat tttgttaaat tctacttcaa accaataccg caaaaattta ttgttatttc
    11461 cggttgccaa cccttgtttt gcatctgcaa catcgttagt taattttcct tgagcaaaat
    11521 cagtgattac gttatcgctt gcccagtaag caatcggcat tcctgggatt tttttgaagt
    11581 tgtcttgatt agtactataa aaataatcgc attcaccatg ctgatgaatt gcttctaacg
    11641 ttttaatttt ttgaacttcc attccacctt taaaatctga taattttata tagcttccat
    11701 cttgttcatt tgaatttttt atcgtaaacg tacaaattgg aacggttgct tcttcaaaag
    11761 ctgaatactc catttgaatc aaactagaaa tgtttctttc attaattaaa aatgagcgta
    11821 acttttcata gctagaaatg aacatccaaa caaatggcgt cataaaggaa gcatatccat
    11881 tatcaatcag catttggcta ttcaatttaa taaacacaga aaatagatct tttttgacat
    11941 cagaatagtt atcatttaca tattttttta atttcttatc cattttatta agataaggcg
    12001 gattagtgac taccgctaca tatttatcaa ctagcatttc agccacgtca agaatgtgca
    12061 agtatttttc ttgtatttcc ttcatcccat aaagaatatc catattcgta aattcgttga
    12121 attttgatat atactctctt acttttccta agtattcttt agttattgcc tgattatttt
    12181 tatcgactaa cttaatgatt gatccaagtt ctttcccatt ttcaaattga tcaaaaatta
    12241 actctttgta ttcattccag cgttcactat acttatcatt gttcaattca ctaatacgct
    12301 cttgatactc gtctgtcact ttaggagaat caataaactc tcttaaattc ggcgatagac
    12361 tttttttcaa aaaaagacga tcgtattgac gccctttcat cataattgca aaataagaca
    12421 attgatacgc tcttttatcg atttcaagac catataaatt gtattcaacg attgcacgtg
    12481 cagcatcacg ctcagaatat cctaacgtaa gataaatttc catcaataca tcgaaggcgt
    12541 aaactaaaat atgcccagac cccattgcgt tatcgataat ttttagttct tctggtttta
    12601 aattacttac agatgttgct tgttcaaaag catatttatc tggaattaag tatttcaact
    12661 tatttcttaa ttcactattg ggataatgat ctaaccaata ctttcctaaa ctattgtcaa
    12721 ccatataacg aacgacccaa tctgtggtaa atagttgagt tgctgccgga atatcttgtt
    12781 ttttgacaat ccctttataa atattcacca cttgattatg tggctcacta ttatagtact
    12841 gatacaacca cccaataatc tctacctgtc caccagcttg aacatagaaa tcatcctctg
    12901 gaatacttaa aagatcagcg attacaccat cttttttaat ataagaagct gtaaacaaaa
    12961 gttctgtata atcttctact tcttcaaata gctcaggtaa gtaatcattc aattgattac
    13021 agatttttat aaaaatcaat tgatacagct tgtccattgc ttctgctgaa ttatctgttt
    13081 tccaagttct gacttgttgc tgttcattac tagataattc ttcaaagata tcggcatcat
    13141 aaatatctgt aataatatct ggctcaattt tcccacttat ttcactagat aatacacgat
    13201 acgtgtcagg taaataatca tttacctcca taaaccgaat ggcaatcaat cggttaaacc
    13261 acgtataagc aacctcttcg attaactctt gaaaagcttc tgaataagaa gtgacagaag
    13321 cacgttgttt tagttctgca atcaatttgt ttctttgcgc tatttctttt ccttgaattc
    13381 ttattcctga tttttcgatt tcttgtaaat tgctatcaat tttcttcaca tcttcaattt
    13441 ttgattcatc aattcctaaa cgttttgctt tagtcattac ttcatcaatt aattttctac
    13501 gggcattgac cgcgaaattc tttatcgctt tcttatccat ttttctttcc cttctatatg
    13561 taacctttga aaagacaggc acctttttat gcctgtcttt tcactagaat tgaatcgtga
    13621 tttctgtatt ttcttgaagt tgttccttca gtttttgatt aatttcagct gttaactcat
    13681 caatatctgc tgctgactta atagttttaa catgaacgtt aagtgttctc attggtactg
    13741 ttttctgttg aatagtaggt tttactatcg ctatttcttc agcttttgct gcagagtcta
    13801 tttcatcttt ttcagtttca cgaatcaagt ttgctgcttc aatttgttta cgtgcttgtt
    13861 cttcttgacg tctgcggttt tcttcattat cgaaacgttg attaaagatc aaatactgtt
    13921 ctgctacgtt taatgataag aaaacaagat catctatagt attcgcttgt tcaatttttg
    13981 ctttcagttc ttgaaattct tggtcaacta ttggtacaaa ttgatcctta aaatcttttc
    14041 cttctaataa tttaaatgtt gcttgttttt cttccgttaa tttatttaat atttcctgac
    14101 tttccaactc taacagttga ttatattgtt caataaattc ttgaatcaac tcatttagtt
    14161 tcatcatatc tgaataagga tcagcctttt tcaaaatggt tttcatctca tgaatcaatt
    14221 gatcaatttc ttggctagaa gtttgacgcc gacttttttc gattaatcta attttatctt
    14281 gtgcattctc ccagattttt ttctgagtac tattttgctt gtggaaattt ttaattttat
    14341 catatgtttc agcccagtcg aataattcat cgcgatattt tttgattgta taaaaaaagt
    14401 cttcaacatc tgacgtttga acaattactt caagatattt ttttatttgc tcatattcat
    14461 aagcactagg gtaggtataa gtgttttgta aatcaaaata agagcgtaaa ttgtctaatt
    14521 gaccttttaa atatttcttg aaattttcca ttgtatgttc attgttattt tcatcaacaa
    14581 tattacgact ttgaaacagt tctctaccga tttcgtcaac caattttttt gcttttggcg
    14641 aaatcaccgc gcttggttca atttttatct tttcactttc tcgtcgtttt tgaataatca
    14701 ttacaatact atttggatca tccatccgtg aaatttgtac attgttataa agtaaatcaa
    14761 ttttaccaga catgaaaagt ttagcgatgc accattccac atcaagttct gtgaatccat
    14821 acggttgttt tgtttcaaaa cgatcacgaa ttgttttcat cgtaatcgtt aggtgatttc
    14881 tcttttgatc attgataaaa ttgaaaattt cctgaactgc ttgctcattt gatggatctt
    14941 caataatttc atttttgctt tctttcacta aatataagat atcttctttt tctttaggcg
    15001 catcaatttg gtttaattta tggtatactt cttcagccat tttctgaagt gctgattgga
    15061 attttgcttt aaagtcggag cctgtcagtt gaatttctgc accgttatag aaaaattttg
    15121 cagatttcaa cagttcattt aattgatcgt tgatacttgc acttagttcc tgtaattcac
    15181 cagatttttc cgaacgaatc gcgtcaaagt tctttactac actcgaataa gtcgttgtta
    15241 aataaagatt cattctagca tacattgcta tatcatcttt aaattcttta tctgatggca
    15301 attctacgaa taataagtta cgggaactct taagcgcaag ttctgcctct tgacctgcta
    15361 aatcagagta tggtgaaata aaattaactg tcatttcagc atttccagct tgcttgtatg
    15421 gacgttgatc taacacttgt gcataagtaa acgtataccg gtttttaaat ttatttccac
    15481 ctgacatgcc taatttttca taggcaaaac tatttcggtc tagaacgccg tcaaaaattc
    15541 gtttagatag ttctacgatc aaatcactat ctgtaatatt ttgattttta atctcacgag
    15601 taatttcctg ttcttcatca gtttggaaaa tataactatc taagttcttc cgcacaaagt
    15661 tttccttaat taaaaggtcc aatgcttctt ctactcgttt tttagtttcc aggcggtctt
    15721 cgttgatatt tcttatcatc aagctcgtaa tattttcaac atttggtttg atggcttgat
    15781 caacatattt aatcatgaat aaaattcgta agacatttac gttaaagtta tctatatcag
    15841 ttggtctttg aatatgagaa ttatttgtag cttgtgtaat cactcggcta attgctgtat
    15901 caatccaacg ttctaacgag taataaaact gatctaaaga aaccaaactg gttgtatcat
    15961 tttccttatt tcgttcagcg gcttctttaa acattgctag taatgaacgt tcaccagacg
    16021 ataagtttga tccttgatag ctgtgattac gaatctcggt tagaatttgc cccaataaat
    16081 tgaattgata gggaataaat ggataaactt ctgcgtaatt ttcagcatct tggaagagct
    16141 ttaattcagg actatcaaca aagagtaatt tatttttcaa ttctgtttga acagtttcaa
    16201 attgtaaggc taaagaatca ttggcaaact cttttttctt caaaatacgt tctttaatta
    16261 cttcatcggc atttgctgaa gataaactca cagttgtggc aaaacgatcc ttgatcttag
    16321 aaaaatcatt atcagaattg ttatcaaagt ttgttacgtg atcaatcgct tcctgacttg
    16381 tcactaatac ccaagcctta ccttcacaaa ttcgacccaa atcttccaca acggtttgta
    16441 actgcaaagt caactcacga ttaccgccga tatattgtcc gacctcatcg actagaaagg
    16501 caatatgatg gtctgcttct ttggaatcaa gatagctttt aactagattt gcaaagtcat
    16561 caatgcttat taaataattt cctgatgctt tttctaaaaa attatcaata gcggcttcac
    16621 tcataaaatc gattttaagt gccgcttctt tgatatcatc ttggatccaa tcaaactcat
    16681 ggcgctcttc ttgccatttt gccccattaa tctcttcaaa agcagctttg aatgcatcaa
    16741 acttttcttc tttaattaac cgacgttcaa aatctgctag atgtggaatc gtgcttaatc
    16801 cttgcatttc gttaaatacc tttaaaaaaa cattcacgat tccgtttttg tcttttttac
    16861 cacctgcatc actttttgaa tcaatattaa acagaataac atctgtattt atttgtgagc
    16921 accgttccat cgttgccaaa gtcagtggat ctttgatttt cccatcttct ttgaagtaat
    16981 caatggcttt cttgtcattt atttctcgat ttgcgagcaa gtaagaaata atttttaaga
    17041 aatgagattt acctgaacca aagaacccct gaatccaaac acccatttta tctgttggtg
    17101 tattgatact ccgttggtag gcatcaaaaa ttgttttaaa gtgtttttgt agttctttcg
    17161 taaccacgta ttcttctaat tcttgttgaa cattttcatc ctttgcttga ccgaccttaa
    17221 taactccttg aatatttcgg tcaattggtt ttgcaaaaat atctttaatt agcatttaat
    17281 ctcctacctt ccatcccaac taaattctaa atgctcgata ataattatca tctttattgg
    17341 tattaagaat tgtcaaactt aattcatcat attcccctgg atacatcatc acaactggta
    17401 cgcgatcaaa tacctgatgc attttattta aaatagagtg cgctctaatt aatggatata
    17461 attcgccaat tcctgtcagg aacacaactg atttttctgg tgtatgggca tccacatatt
    17521 caaaaaatac attttcttcc tcaggtaaat ccattttcaa taaacgacca atgctttcca
    17581 ctaatttttg taaaccacgt cgttcttcca tcttaatcgc tttttcagtg aaaccgcgtt
    17641 tttctagata ctctaacata atcacataga gattataaac tttcacttca taattatcac
    17701 caacagatgt tccagatata tcattaatat aatctcttac aattaactcg tctttcggat
    17761 cgtagtcaaa gatataatag cctacttcat tacctaaacc acgattcaat aaaaaatctt
    17821 ctttttgaat tacttcattc aacttttcca aatgctcttg aatcgataat gacattataa
    17881 aattcctccc agtgctagta caatatcttt acgattcttg tcaactaacc attgaatcaa
    17941 tcgtttatct aataatatcg gagtcattcg tccatattct ttttcccctc tgactaatcc
    18001 tgtttcaatt agattcgttt gataagcggc aataagccgt ttaatcgtat aatctttcca
    18061 ttttgaaata tcttctgaac ttctccgctt ctctgtaaaa aaagaacggt aatcaactgg
    18121 tgttaattgc aaatttcctt tacgtagatt ttcagcatat acctcagtca taaattcatt
    18181 aaaaagatca tcaatcataa ggatggaaat caaattcact aacttttgat tatctatatt
    18241 taactgattg aaaagttgca atccagcttc atccagttga ctaactctcc ttttcacgtt
    18301 ctgatagatt tctttttgac ggttactgct cactgcaaaa aaaatatttt catgttcatt
    18361 tttctcttta atttctatat ctgtatatcc ttctagtcgt aattgaatat ataacttgaa
    18421 ctctgaaaac caaaagcgtt tagaaactag accagcagaa tattttttat tcaatagatt
    18481 gcctcctaat catctataat tcaataagta aacaagtgtt ttttgctcat cgttagcatt
    18541 cattttttag tatctactat acaatacata tttattata
//
LOCUS       DAFCHQ010000056        17449 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000056 DAFCHQ010000000
VERSION     DAFCHQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.056"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..238)
                     /locus_tag="LNC71_001962"
     CDS             complement(<1..238)
                     /locus_tag="LNC71_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467013.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTL"
     gene            complement(255..1460)
                     /locus_tag="LNC71_001963"
     CDS             complement(255..1460)
                     /locus_tag="LNC71_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBK6467014.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            2154..2540
                     /locus_tag="LNC71_001964"
     CDS             2154..2540
                     /locus_tag="LNC71_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6467015.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            2634..2825
                     /locus_tag="LNC71_001965"
     CDS             2634..2825
                     /locus_tag="LNC71_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467016.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            3339..3671
                     /locus_tag="LNC71_001966"
     CDS             3339..3671
                     /locus_tag="LNC71_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6467017.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            3967..4122
                     /locus_tag="LNC71_001967"
     CDS             3967..4122
                     /locus_tag="LNC71_001967"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBK6467018.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            4398..5738
                     /locus_tag="LNC71_001968"
                     /pseudo
     CDS             4398..5738
                     /locus_tag="LNC71_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5979..7595)
                     /locus_tag="LNC71_001969"
     CDS             complement(5979..7595)
                     /locus_tag="LNC71_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK6467019.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            7713..7931
                     /locus_tag="LNC71_001970"
     CDS             7713..7931
                     /locus_tag="LNC71_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467020.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            8126..8587
                     /locus_tag="LNC71_001971"
     CDS             8126..8587
                     /locus_tag="LNC71_001971"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6467021.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(8780..9019)
                     /locus_tag="LNC71_001972"
     CDS             complement(8780..9019)
                     /locus_tag="LNC71_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467022.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(9043..10566)
                     /locus_tag="LNC71_001973"
     CDS             complement(9043..10566)
                     /locus_tag="LNC71_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBK6467023.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(10584..10706)
                     /locus_tag="LNC71_001974"
     CDS             complement(10584..10706)
                     /locus_tag="LNC71_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBK6467024.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(10736..11719)
                     /locus_tag="LNC71_001975"
     CDS             complement(10736..11719)
                     /locus_tag="LNC71_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBK6467025.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(11743..11892)
                     /gene="rpmG"
                     /locus_tag="LNC71_001976"
     CDS             complement(11743..11892)
                     /gene="rpmG"
                     /locus_tag="LNC71_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBK6467026.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(11913..12290)
                     /locus_tag="LNC71_001977"
     CDS             complement(11913..12290)
                     /locus_tag="LNC71_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467027.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(12322..12501)
                     /gene="rpmF"
                     /locus_tag="LNC71_001978"
     CDS             complement(12322..12501)
                     /gene="rpmF"
                     /locus_tag="LNC71_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK6467028.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(12516..12785)
                     /gene="rpsN"
                     /locus_tag="LNC71_001979"
     CDS             complement(12516..12785)
                     /gene="rpsN"
                     /locus_tag="LNC71_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBK6467029.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(13308..15074)
                     /locus_tag="LNC71_001980"
     CDS             complement(13308..15074)
                     /locus_tag="LNC71_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467030.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(15034..16788)
                     /locus_tag="LNC71_001981"
     CDS             complement(15034..16788)
                     /locus_tag="LNC71_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467031.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(16898..>17449)
                     /locus_tag="LNC71_001982"
     CDS             complement(16898..>17449)
                     /locus_tag="LNC71_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBK6467032.1"
                     /translation="IFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIF
                     VYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAI
                     PQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISV
                     LVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         5702 a         3289 c         2588 g         5870 t
ORIGIN      
        1 ataatgtttg tttaaaattg ctaccattaa tatgactgat agattctcta gcttcttcac
       61 ataaactttt tactttatta attactactt tgtcatattc atttaacaca ctattattac
      121 cattacttag acgctcagat tctacgaaac aactcaaatc agaaatttcc caaaaaaatt
      181 ttttgttaaa atccaatttc ctcaactcct ccaaactacg taaatgatat agtctcataa
      241 taaaataaaa cctctcagct aatgttgttg aaattgtcca ctaaacctaa ttgatattgc
      301 ttaggagata cacctgcgta ctttttgaat actctcgaaa agtgtgaaga atctgaaaat
      361 cctaaaccta aagaaacttc actgatagta tggtttgatt tcaacattat tttagccgta
      421 gaaatttttc gcttagagat ataattataa atcgtaatat gcatttcatt cttaaagctt
      481 tctcttaatt tagtttcact tacagaaaaa taattagcta tttcctttac agtaattctt
      541 ttatagatat ttaagccgat gaattgaacg acagaagaaa ttaagggaga aagatgctct
      601 atcttagttt ttccgatttc cttagtataa taaacaatcc cagaatccct cacttttaag
      661 acctcaggta acgatacagc taattcgttt tcttgtatca aagcatctct actttgatac
      721 gcttctatta tatccattcc taatgttata gacgtttgtg ctaacttttc aaaaacaatg
      781 atagaataat ttttttcaga ccgaatcgta tctccactaa cacttggaat tattccatta
      841 ctcagattaa aaaccatgtt ttctaaattt cgaatatctc catttttaac atattccagt
      901 attttatttt cataaagaaa cagatatatt tctggatcgt aattttgtga taacagatta
      961 tctattttga tttgctcgat agttttactt agattttctg tatattcaat taacggttca
     1021 tggaatagat cttctatttt tccagtaaaa aaataatgaa ctaaaatcag aatatctcga
     1081 atgtctccta aagaatacaa agtcaaattt tgcaatgaat tatagagtcg ttctctatca
     1141 gccattgcaa cattcaaatt gttcattttt agtttaaaaa aaactttatc aatattattt
     1201 gctctaaatg gaccaaataa gtaatatctg gttttaaaca tgtatagaag aaacatctca
     1261 ttaaaatcac cagtaaagaa atggaaatct ttagtttttt taatattatc agttagtaac
     1321 tttttataat cataaaaaaa ataagtagtt ctatctgagc gatactcttt tataatactg
     1381 aattcggaat taaaaaccac gataggtaac tttaaacaaa catgaagaga ctttaataag
     1441 acttctttat ctgaaaacac ttttgtttct ctctttcctt tctacatcat taaatagtat
     1501 tttatacgtt atctaaaata ctattcctct gtcaaaataa gctaacttac ttcattataa
     1561 aatatccact tatcgctttc atgtatattc caccgtaaaa agtacatttt atcaccgaga
     1621 actcatcatt tttcatcatg atgaatacta tttacatata tctagttcaa agacaataat
     1681 tattatgatc cgttattatc taagcactat acagataata ggaatttttc gtttctaaat
     1741 attccaattt gggaatccaa taaaaatttt cgttcaaata cgtgtctatt cgaactcttt
     1801 tctagttgtt aattatgaag ggggaagaaa taaaaaatac aatctataca cgaataagaa
     1861 tattatccaa aaggaggttt tagaaaagat ttattatacg taaaaagctc aagacatttt
     1921 cttagccata aggacatgtt tgatatttaa gaagatatat gaattttctt ttctaagtat
     1981 aaagataact atccattatc aactaaaaaa acatttttga aaataaacat cagcagtcct
     2041 atcaatgcat gtgagcttat agaagttatt cgcagcgcag cataattgca ttagcttctt
     2101 ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga ggaggtgtgc aatatgtcag
     2161 ataacgacga acgcgtaaat gcccagttga ttcgattctt tgaaaaggtc attttgaaca
     2221 ccgctaatac ttattataaa aaacaagaag aaattcaaaa acatgaacaa cttgatcaac
     2281 tcccaccata tttgtttact aaagatacta taaaatttaa acaaccgatt aatgttttaa
     2341 atgtgactct ccttgttgaa gatataaaat tagcaaaaat ccttcctttg ttgaaagaac
     2401 ctgaacaaat ttttcttatc gaaaaattta tctttgataa aactgataaa gaaattggaa
     2461 aggttctggg gattacaaga cagggtgcga caaatttaaa acatcgactt tacaaaaaaa
     2521 ttcaaaaaat gctggtttaa tatctgcata ataactaatt ttgtatgctt ctttgctcgt
     2581 tctcctcctt cccaaagaaa tagattataa gtccatggga agaaggtaga tttatgaatt
     2641 ctttaaaaga agatttcatt ttagcaaagg ctggaaatga agaagctgtt gaggcaattc
     2701 tcaaaagatt tagctctttg attcataagc aatcctggag aactggaaaa tatgatcaag
     2761 attgttatca agaatgtatg cttgcaatat atctagctat ctcgaaattc gaaattaaag
     2821 aataactcaa ctaagggagt ctgtgacata agtaaaaaga tagtttcaaa tttcgaacaa
     2881 taaatttgaa actatcttct ttataattat aaagtatcga ttttacagcg ttctataatg
     2941 attcatagaa tctaaaacga atgattttaa ttatcaacgt tttttaattc gtttttagct
     3001 taaaaataac ttttgtcaca gtctcactcc taaaattact ttatttacgt atgaagtggg
     3061 caggaggtcg aatccagaac tgggctatgg tacttcaacg aggacttctc ctctagaatt
     3121 gaaaagcatg cgatttacct tccgtaacga cggtagattt attcaactat gtgtccaatc
     3181 aaaatttgtc tgatttcaaa aatagtcaag agaattttgg ttgttaaaaa ctcttatcta
     3241 tcaacttcca cagtttagtt gacaaaccct gatactaagt tgggctatga tacacttttc
     3301 ttgactatca gttacgttac cggaaggaat gaactctatt gagaaaaaca tatgatcgta
     3361 aatttaaact aaagatttca caggatatcc ttgagaaaaa agtaactacc aagaaaattg
     3421 ctgaagaata caatatctct cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat
     3481 acgggaaaaa tgcttttgct ggacaaggaa aaagattacc tgataaagca gatttctaca
     3541 ttcttgaaca ggaaaataaa cgactaaaag aggaagacga gtgttctacg aacacaaaga
     3601 tcgatacgga tctccaggga tcactcaaaa actttacgat gaaggaatcg aaatcaataa
     3661 acgtgttgta gctgttctta tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg
     3721 caaatcttct catggtagag aaaaaacaat caaagaaatg gtaaaaaaat gttgaatcga
     3781 taatttgaac agaaccaaat tgatgctgtt tgggtgatgg atattactta cattccatgt
     3841 aatgacggtc gcctctattt gtccacctat attgacttag caactcgtat tctacgttac
     3901 tatatggtag attcacacat gaaaaaagag attgtcgtcc gtccattaca aatttattaa
     3961 ggagaattgc ctacggtaat tcattccgat agaggtagcc agcatcggtc ttttatttat
     4021 caagagctgt tgaacgacca ccacatcaca cattcagtga gccaaactgg gactcctgtt
     4081 gacaatgcag taattgaagc gtttcatcga tcaatcaaat gagaattaat cgactccaac
     4141 aaatataaaa gtaaaataga gatgaaggtg ttaatccaag attatttaga agactactac
     4201 ccaaacaaaa gaatccatac aaaatttatg atgactcctt gacaatttga agcgaagaaa
     4261 ataagggatc tataatatct agtgttggtg acgccttctg ttgccaatac tttttctttt
     4321 ttctaataaa cgcaaacaga caccttaaaa tcctttagaa taaagtcgac gaaaaccatt
     4381 caaaggagtg tttcaagatg tcctattcag agtttaccaa agagttacta gatattctag
     4441 acctaaatct tatctttcat gaagatgcct ttcgcaaaga gcgaatcaat gacgaaacat
     4501 gttttgtttt tgatggcaca ttgacctatc aaccggagga atgctttcat cgccactatc
     4561 aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt atccatctta ttgaatgatg
     4621 tcagtaacta caagaccatt ctgcggatta acaaacagcg cttcaaatgt aaacactgtg
     4681 gaaagacttt tttagcagaa gattcagtta gtgatcgtcg ctgctccatt gctcgcagag
     4741 tcaaacaagc cattcttgag ctattaagcg aacctctttc tatgtctttg atcgcacgta
     4801 tgaagcacat ttctccgacc actgtcattc gaatacttcg cagccttcgg cctaaaacca
     4861 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
     4921 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
     4981 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
     5041 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
     5101 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
     5161 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
     5221 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
     5281 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
     5341 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
     5401 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
     5461 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
     5521 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
     5581 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
     5641 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
     5701 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
     5761 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
     5821 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     5881 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     5941 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     6001 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     6061 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     6121 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     6181 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     6241 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     6301 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     6361 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     6421 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     6481 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     6541 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     6601 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     6661 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     6721 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     6781 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     6841 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
     6901 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
     6961 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
     7021 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
     7081 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
     7141 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
     7201 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
     7261 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
     7321 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
     7381 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
     7441 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
     7501 tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
     7561 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
     7621 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
     7681 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
     7741 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
     7801 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
     7861 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
     7921 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
     7981 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
     8041 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
     8101 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
     8161 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
     8221 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
     8281 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
     8341 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
     8401 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
     8461 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
     8521 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
     8581 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
     8641 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
     8701 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
     8761 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
     8821 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
     8881 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
     8941 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
     9001 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
     9061 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
     9121 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
     9181 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
     9241 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
     9301 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
     9361 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
     9421 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
     9481 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
     9541 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
     9601 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
     9661 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
     9721 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
     9781 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
     9841 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
     9901 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
     9961 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
    10021 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
    10081 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
    10141 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
    10201 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
    10261 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
    10321 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
    10381 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
    10441 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
    10501 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
    10561 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
    10621 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
    10681 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
    10741 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
    10801 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
    10861 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
    10921 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
    10981 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
    11041 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
    11101 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
    11161 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
    11221 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
    11281 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
    11341 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
    11401 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
    11461 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
    11521 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
    11581 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
    11641 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
    11701 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
    11761 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
    11821 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
    11881 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
    11941 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
    12001 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
    12061 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
    12121 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
    12181 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
    12241 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
    12301 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
    12361 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
    12421 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
    12481 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
    12541 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
    12601 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
    12661 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
    12721 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
    12781 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
    12841 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
    12901 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
    12961 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
    13021 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
    13081 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
    13141 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
    13201 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
    13261 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
    13321 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
    13381 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
    13441 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
    13501 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
    13561 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
    13621 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
    13681 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
    13741 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
    13801 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
    13861 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
    13921 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
    13981 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
    14041 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
    14101 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
    14161 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
    14221 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
    14281 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
    14341 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
    14401 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
    14461 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
    14521 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
    14581 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
    14641 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
    14701 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
    14761 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
    14821 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    14881 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    14941 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    15001 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    15061 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    15121 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    15181 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
    15241 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    15301 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    15361 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    15421 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    15481 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    15541 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    15601 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    15661 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    15721 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    15781 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    15841 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    15901 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    15961 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    16021 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    16081 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    16141 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    16201 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    16261 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    16321 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    16381 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    16441 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    16501 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    16561 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    16621 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    16681 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    16741 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    16801 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    16861 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    16921 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    16981 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    17041 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    17101 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    17161 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    17221 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    17281 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    17341 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
    17401 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaat
//
LOCUS       DAFCHQ010000057        16647 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000057 DAFCHQ010000000
VERSION     DAFCHQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.057"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..546
                     /locus_tag="LNC71_001983"
     CDS             <1..546
                     /locus_tag="LNC71_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBK6467033.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="LNC71_001984"
     CDS             735..1403
                     /locus_tag="LNC71_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBK6467034.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1833)
                     /locus_tag="LNC71_001985"
     CDS             complement(1603..1833)
                     /locus_tag="LNC71_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467035.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1960..3342)
                     /locus_tag="LNC71_001986"
     CDS             complement(1960..3342)
                     /locus_tag="LNC71_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK6467036.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="LNC71_001987"
     CDS             complement(3498..4685)
                     /locus_tag="LNC71_001987"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBK6467037.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="LNC71_001988"
     CDS             complement(4692..5723)
                     /locus_tag="LNC71_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBK6467038.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5739..7193)
                     /locus_tag="LNC71_001989"
     CDS             complement(5739..7193)
                     /locus_tag="LNC71_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6467039.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            7368..8273
                     /locus_tag="LNC71_001990"
     CDS             7368..8273
                     /locus_tag="LNC71_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6467040.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="LNC71_001991"
     CDS             8364..9041
                     /locus_tag="LNC71_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6467041.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9038..9208
                     /locus_tag="LNC71_001992"
     CDS             9038..9208
                     /locus_tag="LNC71_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467042.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9322..10218
                     /locus_tag="LNC71_001993"
     CDS             9322..10218
                     /locus_tag="LNC71_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6467043.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="LNC71_001994"
     CDS             complement(10273..11643)
                     /locus_tag="LNC71_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6467044.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="LNC71_001995"
     CDS             11881..12627
                     /locus_tag="LNC71_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBK6467045.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="LNC71_001996"
     CDS             12624..13463
                     /locus_tag="LNC71_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBK6467046.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14414
                     /locus_tag="LNC71_001997"
     CDS             13464..14414
                     /locus_tag="LNC71_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBK6467047.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14555..15307
                     /locus_tag="LNC71_001998"
     CDS             14555..15307
                     /locus_tag="LNC71_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467048.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15326..16477
                     /gene="queG"
                     /locus_tag="LNC71_001999"
     CDS             15326..16477
                     /gene="queG"
                     /locus_tag="LNC71_001999"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBK6467049.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5406 a         3099 c         3338 g         4804 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgctaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgttactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg atctactgca
     5761 tcgtgaagtt catcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctgcattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact tttcttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagcgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa
    14041 gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt
    14101 atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc
    14161 aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg
    14221 ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg
    14281 gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag
    14341 attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa
    14401 caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa
    14461 gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc
    14521 cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata
    14581 tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca
    14641 agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac
    14701 aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt
    14761 agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg
    14821 cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt
    14881 catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat
    14941 tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg
    15001 gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta
    15061 ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag
    15121 tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc
    15181 aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc
    15241 cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag
    15301 ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa
    15361 acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga
    15421 atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga
    15481 tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact
    15541 tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc
    15601 tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat
    15661 cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc
    15721 gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt
    15781 tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga
    15841 gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg
    15901 tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc
    15961 ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa
    16021 aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca
    16081 aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaaa tatatccaaa
    16141 attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc
    16201 tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct
    16261 aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat
    16321 cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct
    16381 tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa acgaattgga
    16441 acatgcccag aaattattgc ggagaaagtt aaaataaaaa aatagttaac attattcaaa
    16501 gtttttgtcc agtaattgtt gtaacaatta ctggtttttt tgtatttttt acattttttt
    16561 aagagaattt gataaaaatc aaaaattttt ttgctttttt ttatggatta ttgcagtgaa
    16621 tgttacagaa tctgcgtttt tcattca
//
LOCUS       DAFCHQ010000058        15742 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000058 DAFCHQ010000000
VERSION     DAFCHQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.058"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(24..722)
                     /locus_tag="LNC71_002000"
     CDS             complement(24..722)
                     /locus_tag="LNC71_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBK6467050.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="LNC71_002001"
     CDS             complement(715..2067)
                     /locus_tag="LNC71_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBK6467051.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="LNC71_002002"
     CDS             complement(2290..3690)
                     /locus_tag="LNC71_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBK6467052.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="LNC71_002003"
     CDS             3851..4588
                     /locus_tag="LNC71_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBK6467053.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="LNC71_002004"
     CDS             4713..5192
                     /locus_tag="LNC71_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBK6467054.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="LNC71_002005"
     CDS             5189..5956
                     /locus_tag="LNC71_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467055.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="LNC71_002006"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="LNC71_002006"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBK6467056.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="LNC71_002007"
     CDS             complement(6859..7461)
                     /locus_tag="LNC71_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6467057.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="LNC71_002008"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="LNC71_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="LNC71_002009"
     CDS             8142..8726
                     /locus_tag="LNC71_002009"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBK6467058.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="LNC71_002010"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="LNC71_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBK6467059.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="LNC71_002011"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="LNC71_002011"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBK6467060.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="LNC71_002012"
     CDS             10627..10806
                     /locus_tag="LNC71_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467061.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="LNC71_002013"
     CDS             10828..11853
                     /locus_tag="LNC71_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBK6467062.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="LNC71_002014"
     CDS             12028..13272
                     /locus_tag="LNC71_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBK6467063.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="LNC71_002015"
     CDS             13425..14372
                     /locus_tag="LNC71_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6467064.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="LNC71_002016"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="LNC71_002016"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBK6467065.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="LNC71_002017"
     CDS             complement(15274..15516)
                     /locus_tag="LNC71_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467066.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="LNC71_002018"
     tRNA            15637..15709
                     /locus_tag="LNC71_002018"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tt
//
LOCUS       DAFCHQ010000059        15437 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000059 DAFCHQ010000000
VERSION     DAFCHQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.059"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1018..1359)
                     /locus_tag="LNC71_002019"
     CDS             complement(1018..1359)
                     /locus_tag="LNC71_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBK6467067.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1359..1589)
                     /locus_tag="LNC71_002020"
     CDS             complement(1359..1589)
                     /locus_tag="LNC71_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6467068.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1876..2214
                     /locus_tag="LNC71_002021"
     CDS             1876..2214
                     /locus_tag="LNC71_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467069.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2419..2658)
                     /locus_tag="LNC71_002022"
     CDS             complement(2419..2658)
                     /locus_tag="LNC71_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK6467070.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2634..2981)
                     /locus_tag="LNC71_002023"
     CDS             complement(2634..2981)
                     /locus_tag="LNC71_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBK6467071.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3713..4294)
                     /locus_tag="LNC71_002024"
     CDS             complement(3713..4294)
                     /locus_tag="LNC71_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6467072.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4477..5103)
                     /locus_tag="LNC71_002025"
     CDS             complement(4477..5103)
                     /locus_tag="LNC71_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6467073.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5125..6456)
                     /locus_tag="LNC71_002026"
     CDS             complement(5125..6456)
                     /locus_tag="LNC71_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBK6467074.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6611..7012)
                     /locus_tag="LNC71_002027"
     CDS             complement(6611..7012)
                     /locus_tag="LNC71_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBK6467075.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7098..7304)
                     /locus_tag="LNC71_002028"
     CDS             complement(7098..7304)
                     /locus_tag="LNC71_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467076.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7399..8901)
                     /locus_tag="LNC71_002029"
     CDS             complement(7399..8901)
                     /locus_tag="LNC71_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBK6467077.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8919..9374)
                     /gene="argR"
                     /locus_tag="LNC71_002030"
     CDS             complement(8919..9374)
                     /gene="argR"
                     /locus_tag="LNC71_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBK6467078.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9472..10089)
                     /locus_tag="LNC71_002031"
     CDS             complement(9472..10089)
                     /locus_tag="LNC71_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBK6467079.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10310..11683
                     /locus_tag="LNC71_002032"
     CDS             10310..11683
                     /locus_tag="LNC71_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6467080.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11758..12870
                     /locus_tag="LNC71_002033"
     CDS             11758..12870
                     /locus_tag="LNC71_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBK6467081.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12942..14579)
                     /locus_tag="LNC71_002034"
     CDS             complement(12942..14579)
                     /locus_tag="LNC71_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBK6467082.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14747..15355)
                     /locus_tag="LNC71_002035"
     CDS             complement(14747..15355)
                     /locus_tag="LNC71_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6467083.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4275 a         3166 c         2692 g         5304 t
ORIGIN      
        1 ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat cattaataaa
       61 tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca acatataccc
      121 tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc atagacactt
      181 ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa aaagtgactc
      241 aaaataaaaa aaaccttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc
      301 tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat
      361 caattagaga ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga
      421 ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg
      481 tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg
      541 tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg
      601 cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa
      661 attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt
      721 agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc
      781 gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa
      841 cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt
      901 aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct
      961 ttttttaatt tcgttatgtt tagcatataa aaatattata gagtttactt acaatgctta
     1021 aaacatatac tgtataatct gatcaatttt agccatgtct tgcaatggta agttttctat
     1081 ttttttgagt tttcgttcct taaaatcaag agattttaat tgtgtaataa ttacttgtcc
     1141 ttttatatct agctcttcag gtaatgagta acgtgtcggt aaattcttta tagtggaagt
     1201 aattggacat atgaccgaaa acattgtttt acggttaaat tcataacgag agacgactag
     1261 tcctggtcgt cttttttgaa tttcttttcc tacggaggga tcaaaatcaa tccatacaat
     1321 atcacctttt ttaggaatat aaattctttc ttctgacact ataaaatttc tctcccttca
     1381 ggagttaaat cttcccattc gtctttctca taatactcgg cctcttcccc accactaaat
     1441 ggatcttcaa tttttggaac taacgtaatt gttccatcat cagaatacac aacgatatat
     1501 tcttggtttt ctgaaggttt ctttccgtta tcagaaggta aagttaccac tacagaactt
     1561 ccttgtaatc tagattttgt aacaagcatt atcatcactc ccttaataaa agtatactat
     1621 ataacactaa gtaacgcaaa attccccttt gtaaactatc tagaaaattg ttttgtttaa
     1681 agtatatctt attggatata aatagaaata aattgtagcc agttcatttt ttctttaact
     1741 aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa atactatagc
     1801 taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca
     1861 acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt ttgctctgac
     1921 aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac tgttcggatc
     1981 aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag acaagacttt
     2041 gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt acatgatcca
     2101 actcatatca atgtggataa atggcaacct cttatctata aattcagaga atatacaact
     2161 gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc ataatgataa
     2221 acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa tagaagctat
     2281 acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat gagcatttct
     2341 tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg ccgctctttc
     2401 tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct gctgagtaat
     2461 gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt cagtcaagac
     2521 aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag tcattatgat
     2581 tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat acactatttt
     2641 ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcattgg gaaaacacaa
     2701 cgattttgca aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacgggca
     2761 ttaatcatca attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg
     2821 aggatgtttt cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca
     2881 gctgctaatt ctgctgcctg cttcggttgt ttcgtctggg cttctcgata ataattctga
     2941 aggagctctg actctagatc aaaaaaataa cgaccacaca tcctaatccc tcccaatctt
     3001 tttttctatc atacactgca acaagaagtt cgcaccaaca aaacgctttc aatttttgta
     3061 gaactaaaaa agttgtgaca agtgacaaac tctccagtga atggtattct cctgccaact
     3121 cctcggtcac aagtcacaat attcaacaaa catgagaagc tgctttttga aattgttcct
     3181 tgtgactcgg agtgaaacgg caggcttacg acctctgggt aaacggtgtt caaaagccag
     3241 tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gctattttca caaaattttt
     3301 cttaatactc gaaactaagc gactttttca caacctcttc cttaaacggt gttcaaaagc
     3361 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agcagctatt tttatttggc
     3421 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctccactaaa cggtgttcaa
     3481 tcagctaacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     3541 tgtttctcta atactcaggt caaagcgctg atttcacaac ctctaataca cggtgttcca
     3601 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
     3661 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa
     3721 ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata
     3781 gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg
     3841 cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt
     3901 tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca
     3961 ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct
     4021 catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag
     4081 catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg
     4141 ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag
     4201 aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt
     4261 tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat
     4321 agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt
     4381 gccaaattat ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt
     4441 gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc
     4501 ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc
     4561 ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctac
     4621 caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt
     4681 gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt
     4741 atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc
     4801 ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc
     4861 aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata
     4921 atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat
     4981 attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc
     5041 tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa
     5101 catggtggat tctccattcg ttatttattt taatacatca tttagagctt cactcattgt
     5161 cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag
     5221 cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa
     5281 gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc
     5341 tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc
     5401 ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac
     5461 agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg
     5521 ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca
     5581 aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag
     5641 tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc
     5701 caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc
     5761 tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc
     5821 atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc
     5881 agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc
     5941 caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat
     6001 cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat
     6061 tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt
     6121 attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc
     6181 agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc
     6241 tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt
     6301 agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac
     6361 gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc
     6421 aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct
     6481 accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta
     6541 tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc
     6601 ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt
     6661 aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat
     6721 cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag
     6781 agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta
     6841 ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt
     6901 ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt
     6961 ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc
     7021 tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct
     7081 ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa
     7141 aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc
     7201 ttttctacat aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc
     7261 ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc
     7321 tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag
     7381 cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa
     7441 taattttgct aattttacta acgccaactt gttctttccg aattcatcat tttctatctg
     7501 taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg
     7561 ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc
     7621 ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc
     7681 gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg
     7741 ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat
     7801 gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc
     7861 gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt
     7921 tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag
     7981 atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat
     8041 tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag cactgacaac
     8101 tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac
     8161 agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa
     8221 tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac
     8281 tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt
     8341 tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta aatcttgcgc
     8401 ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg
     8461 ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa
     8521 ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca
     8581 cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc
     8641 gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc
     8701 gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt
     8761 cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt
     8821 caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg
     8881 cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc
     8941 ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca
     9001 ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca
     9061 tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa
     9121 acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta
     9181 aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga
     9241 atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc
     9301 agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc
     9361 tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt
     9421 tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt
     9481 ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct
     9541 gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc
     9601 ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta agatttgggc
     9661 acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt ccggaagttt
     9721 gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat
     9781 catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg
     9841 cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca
     9901 atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg
     9961 tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg attttttagg
    10021 aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat
    10081 tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt
    10141 tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt
    10201 aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta
    10261 taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca
    10321 aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt
    10381 attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac
    10441 gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc
    10501 gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct
    10561 gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt
    10621 tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat
    10681 cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac
    10741 ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg
    10801 gaatcatatc ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta tcggctggtt
    10861 tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg
    10921 aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag
    10981 ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa
    11041 agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt
    11101 gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt
    11161 ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc
    11221 agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa
    11281 gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact
    11341 cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat
    11401 cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca
    11461 aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc
    11521 attagtagcg gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc
    11581 tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt
    11641 tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg
    11701 tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg
    11761 gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat
    11821 acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc
    11881 gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct
    11941 aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt
    12001 tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt
    12061 cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct
    12121 gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt
    12181 ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa
    12241 caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc
    12301 aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt
    12361 aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc
    12421 ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga
    12481 gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac
    12541 gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac taagagtaca
    12601 gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt
    12661 acccgtgctt atacgtcaga tttttcccca tttttcggcg tactgccaga tgatccaagc
    12721 ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat
    12781 ctattagcca acttcttaaa tactggtcat tggcctacgg aagattatca aaaggacatc
    12841 tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga
    12901 gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc
    12961 aaattctcca aactttcttt tgcttcatct ggcaagagat cttttcctat ctgagcttca
    13021 atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca
    13081 aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac
    13141 ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg
    13201 atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa
    13261 aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca
    13321 aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat
    13381 cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata
    13441 attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga
    13501 tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt
    13561 atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga
    13621 caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag
    13681 taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca
    13741 tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca
    13801 gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc atattgtttc
    13861 atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa
    13921 gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga
    13981 gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag
    14041 atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa
    14101 ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc
    14161 agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa
    14221 aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt
    14281 tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca
    14341 tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt
    14401 gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttccta
    14461 tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg
    14521 gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg
    14581 gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt
    14641 atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa
    14701 cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt
    14761 ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt
    14821 ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt
    14881 gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg
    14941 cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag
    15001 ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg
    15061 tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg
    15121 ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag
    15181 caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat
    15241 cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg
    15301 cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa
    15361 actccttttt ttctccgttt tttattcttt cgacgctatt aactttatca tttagaaata
    15421 tttcataggt aaaagaa
//
LOCUS       DAFCHQ010000060        15255 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000060 DAFCHQ010000000
VERSION     DAFCHQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.060"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            248..1021
                     /locus_tag="LNC71_002036"
     CDS             248..1021
                     /locus_tag="LNC71_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK6467084.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="LNC71_002037"
     CDS             1018..1785
                     /locus_tag="LNC71_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBK6467085.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="LNC71_002038"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="LNC71_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBK6467086.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="LNC71_002039"
     CDS             3750..4070
                     /locus_tag="LNC71_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBK6467087.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="LNC71_002040"
     CDS             4070..4420
                     /locus_tag="LNC71_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBK6467088.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="LNC71_002041"
     CDS             4587..5525
                     /locus_tag="LNC71_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBK6467089.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="LNC71_002042"
     CDS             5540..6370
                     /locus_tag="LNC71_002042"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBK6467090.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="LNC71_002043"
     CDS             6414..7439
                     /locus_tag="LNC71_002043"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK6467091.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="LNC71_002044"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="LNC71_002044"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK6467092.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="LNC71_002045"
     CDS             8517..8957
                     /locus_tag="LNC71_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBK6467093.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="LNC71_002046"
     CDS             8960..9562
                     /locus_tag="LNC71_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBK6467094.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="LNC71_002047"
     CDS             complement(9625..10728)
                     /locus_tag="LNC71_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBK6467095.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="LNC71_002048"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="LNC71_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBK6467096.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="LNC71_002049"
     CDS             12294..12689
                     /locus_tag="LNC71_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467097.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="LNC71_002050"
     CDS             12689..12940
                     /locus_tag="LNC71_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467098.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="LNC71_002051"
     CDS             12983..13378
                     /locus_tag="LNC71_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467099.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="LNC71_002052"
     CDS             13375..14631
                     /locus_tag="LNC71_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBK6467100.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="LNC71_002053"
     CDS             14643..14966
                     /locus_tag="LNC71_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467101.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="LNC71_002054"
     CDS             14959..15225
                     /locus_tag="LNC71_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467102.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAFCHQ010000061        15217 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000061 DAFCHQ010000000
VERSION     DAFCHQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.061"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(118..348)
                     /locus_tag="LNC71_002055"
     CDS             complement(118..348)
                     /locus_tag="LNC71_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467103.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(784..1350)
                     /locus_tag="LNC71_002056"
     CDS             complement(784..1350)
                     /locus_tag="LNC71_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBK6467104.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1350..2306)
                     /locus_tag="LNC71_002057"
     CDS             complement(1350..2306)
                     /locus_tag="LNC71_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBK6467105.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2306..2962)
                     /locus_tag="LNC71_002058"
     CDS             complement(2306..2962)
                     /locus_tag="LNC71_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBK6467106.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3332..5350
                     /locus_tag="LNC71_002059"
     CDS             3332..5350
                     /locus_tag="LNC71_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6467107.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5420..5626
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5777..8191
                     /locus_tag="LNC71_002060"
     CDS             5777..8191
                     /locus_tag="LNC71_002060"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBK6467108.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8539..10092
                     /locus_tag="LNC71_002061"
     CDS             8539..10092
                     /locus_tag="LNC71_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK6467109.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10473..12257)
                     /locus_tag="LNC71_002062"
     CDS             complement(10473..12257)
                     /locus_tag="LNC71_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467110.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12272..13993)
                     /locus_tag="LNC71_002063"
     CDS             complement(12272..13993)
                     /locus_tag="LNC71_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467111.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            14093..14854
                     /locus_tag="LNC71_002064"
     CDS             14093..14854
                     /locus_tag="LNC71_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6467112.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4954 a         2794 c         2987 g         4482 t
ORIGIN      
        1 tatcttcgat tgctaaaaag aaaacttctg aatcccacac cttataaaaa tctttgacaa
       61 atctattcga aggatcaaat ttaaaccata attgcgtctc attaaaaagc ataaccatta
      121 ctccgatcta tttttaaact tagtctctac ctctaatata tatcgagttt ttattttgcc
      181 ttcagagaat actgtttctt tttttgcagt tacaggttgt ttattttcgg aaaaagctaa
      241 tatagctaaa attgaaacat ccatctggaa tttatctttt ttactgcttt gatcataaaa
      301 gtctgcatat tcatcactga tattttttct aataaattct tccatcataa aaatcacctc
      361 aaaacgatta tacgaactta cgttcttgtt gtaaagcgat atttgagggg caaaaaaggg
      421 gcataatttg actgatatta agttagctat tgatatatga atgacataca aaaatataaa
      481 atcttttaaa aatgcttata tatcaatgtt tttgccattc ttttatatat ctgttatatg
      541 tcaaaaaaat gtgttcaatt tcaatttgta tttgaataaa gaggttgtga aaaagctgtc
      601 ctgcatcaag taataagaac agccaatcaa aaacagcttc tcatgttttt gatttggttg
      661 gacttattac cgcagatata agcttttgag caccgtgaat aaagaggtta tgaaatcaac
      721 gctctaacct gagtattaga gaaaaaaacg caaaacatat catttatgtt ttgcgttttt
      781 tatttatctt tttttctcaa tacaatataa aataggcggc tggttctttt ggttgataaa
      841 ccgataattc agcacattat atgtttcctg tggcagtttg gaacaaaaag aatcaaccat
      901 atccagttct ttttctccac cttcatgacc atagtagacg acaaggatca atcgtccgcg
      961 aggcacaagt cgtttcaata tttcttccat agctgtgcgt gtagtctctg gcatagtgat
     1021 gattgcttta tcactttttg ggagataccc tagattgaaa atccctgctt tgagattttg
     1081 ttgttcagct atcacttctc ctaacgtttc gtggccttgt agaaacaatg agacacgttc
     1141 ttctaactga cgctgttcta gcctttcacg agtagcatcg attgcttgtt gctggatatc
     1201 aaaactatag acatgaccag tttttccgat gtgttctgct agaaaaaccg tatcgtggcc
     1261 attgcccatc gttgcatcta cgacgtgatc ccctggttgc aagatttctt gcagtaatgt
     1321 atggctgaaa tgcaaagcag tttgcaacat cagacgctca cctttttccg tacatcatat
     1381 tttccttgga agctatctct gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat
     1441 tttttcaagc tccacattgg tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga
     1501 tggatgatga tctcttttgg tatcatttcc agctggtcgc aaatgacatt gacatagtca
     1561 ggacggctca ttaattgaag gcgaccttca tgatagtccc gcaacattct cgttttgcgc
     1621 atcagatgca tcaaatggag cttgattcct tggatatctg aatctaaaat ggtgcgtctg
     1681 acattttctt tcatcatttc taaactttcg cctggtaatc cgttgatcaa atgcgtacaa
     1741 acacggatat tatgtttgcg taatttagcc acgccatcta aataagtttg ataatcatgt
     1801 gctcggttga tcagttttga agtttcttca aaagttgttt gcaatccaag ttctacccat
     1861 aaatacatcc gctcgttcag ttcagcaagg taatcaacta cttcatctgg cagacaatcc
     1921 ggtcgtgtac cgactgataa tcctacgaca cctggtaaat tgactacttg ctcaaatcgt
     1981 tctttgatta tttcaactgg cgcatgggtg tttgtaaaat tttggaaata aacgatgtat
     2041 tgttggacat gaggccattt ttgatgcatc atgtcgattt ctttctggaa ttggattgga
     2101 agagggtctt caggtgcaac gatcatgtct cccgaaccag atacactgca aaaagtacaa
     2161 ccgccatggg caacagtccc atctcgattc ggacagtcaa atcctccatc gatcggcacc
     2221 ttgaaaattt tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg atatctttta
     2281 tttccttctg aataaggaaa agtcattaaa agtccttctt tctgtaagag ttatctattt
     2341 gttttctttt ttctctatac actggataag tcaggcacag ccaagaaaga ctgagtaagt
     2401 aaccgccgat gatatcagta ggaaaatgaa cgcccagata aatgcggctt atcccgacac
     2461 ttactataat aaacatcatc agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa
     2521 aagctggaag cagaaatagt aaagaaccgt aaagaatcat actgcctgct gcatgtccac
     2581 tagggaaact gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc tctcgcatga
     2641 aaaatagttt gatcaccggg ttgatgattc cagcaattac aataaatcca gaaacgaacc
     2701 aaaatgtttc tgcccttttt ttcttatacc acatccataa agccgcacaa gcaaataaaa
     2761 tgatgacggt aagaggatta ccgaatttag tcataaagag ttggtagctg ttcagatcag
     2821 gataagaagc tctgacaatg cctgtaaacc actgatcgaa accagaaagc cacgacagat
     2881 ggaactttac gatccagccc aaaatgaaaa acaggacaag aaagaagcta ccagccacct
     2941 gaaatgatgt tttatatttc atacaattcc tcctttgaag caatgactta ttatagcatt
     3001 tttggtccag cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc tctttgaata
     3061 accaatattc ttataaaaaa aataactgta atcggaaaga cattttttgg taatgcaaat
     3121 gaaatgttag ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg
     3181 taggaagaac ttgaaaatga acagaagttg ttcattacgg taaattacaa tatgattaca
     3241 aaaaacaaca aagctcttaa ataactttta ataaatatta agagaagtta taattattaa
     3301 taatagataa attgaagttg gaggatagaa gatggaaaac attgcaagaa aagaacggag
     3361 acgtttagaa cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac
     3421 tgcgatggta ggctgttctg tcgctgcccc attagtacag ccggttcctg ttcaagcgga
     3481 tgaaacacct gttcagttcg gcgcaagaat caatacagcg gcatttatca cagaaattgc
     3541 tacttatgct caaccaatcg ctcaagccaa cgatctatat gcatcagtga tgatcgcaca
     3601 agcagttgta gaaagcggtt ggggcagtag tgcgttatct caagcacctt attataatct
     3661 ttttgggata aaaggcagct atcaaggaca aaccgtctac atggatacgc ttgaattttt
     3721 aaataatcaa tgggtaaata aaaaggagcc tttccgacaa tatccttctt ttgctgaatc
     3781 tttcaatgac aatgcctatg tacttagaaa tacttcattt ggcaacggtt attactatgc
     3841 cggtacttgg aagagcaaca cgaaatccta tacagatgcg acagcttgtt tgacagggcg
     3901 ttatgcgact gatcctggtt acgcaggcaa attaaataat atcatcgcaa cttacggatt
     3961 gacccaatat gatacaccag ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa
     4021 taatactgga agtgcaggta gtactggcgg ttcaagcaat tctggtaata caaactcagg
     4081 ttcagcaact tcgaccactt atactgtcca atctggtgat tctgtttggg gaatctcaaa
     4141 taaattcggt atcacaatgg atcaattgat ccaatggaac aacatccaaa acaatttcat
     4201 ttatccagga caaaaactga caatcaaagg tagccaggaa aatggctctt cgacgaataa
     4261 ttccggtaac aataccaatt cttcaggaaa tgctggtaca tcaaacggag gacaaacaac
     4321 tggtgcgaaa tacactgttc aatctggtga ttctgtttgg aagatctcaa atgatcacgg
     4381 aattacgatg gctcaattga ttgaatggaa caatatcaag aacaatttcg tttatccagg
     4441 acaacagcta atcgtatcaa atggcggttc ttctaatgct ggagcaaata ctggctcaaa
     4501 tactgggaat acaagtggct ctagttcgtc cagctcaagt tcttctaata cagcttcagg
     4561 aacgaaatat acagttaaag ccggagactc tgtttggagc gtctcaaata aatacggaat
     4621 tacgatggct cagctaatcc aatggaacaa tatccaaaat aactttatct acccaggaca
     4681 acaactgact gtttcaaaca gtggatcaac aaacagcacg tcaggtaata caggttcgtc
     4741 aaccaataca aataacagca gttcagttaa tactggcagc agttcgtctc aaggcactta
     4801 taccgtgaaa gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca tcacgatgga
     4861 tcagttaatc gaatggaata atatcaaaaa caacttcatt tatccaggac agcaattgat
     4921 cgttaaaggt ggaacttcgg caaatacaaa tacaggttca actacttctg cagcgaaacc
     4981 aaatacaccg aatacgtctg ctacgacaag tacatcatcc actggtaata cgatgtatac
     5041 cgtgaaagcg ggcgagtcag tttggagcgt agcgaacaaa catcatatca cgatggatca
     5101 gttaatcgaa tggaataata tcaaaaataa tttcatctat ccaggacagc aagtgattgt
     5161 gaagaaaggt tcatctcaat caacaaacca acaatcagtt actggttcga aaacctatac
     5221 tgtgaaatct ggagaatctg tttggagtgt tgctgacagt catggaatta caatggctca
     5281 actaatcgaa tggaacaata ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt
     5341 agctaaataa aatagctgtt cgaagaaata acgattgaat tccacaaaaa tatttgttac
     5401 tataaggcta cttaaatatg acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc
     5461 agagagcgca tggctggtga gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc
     5521 cttgctgaat acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt
     5581 tcactaaggt aaaattaggt ggtaccgcgt agattacgtc ctataaaaag agcaatcttt
     5641 ttataggatt tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa
     5701 ataaacagat tctgccggtt ttttcgttat atttataata gaaacaagtg ttttcaatag
     5761 aacaggagga cagcaaatga gttacaatca taaagaaatt gagaaaaaat ggcaaaaata
     5821 ctgggcaaaa acgaatactt tcaacaccca tgatgatcct gaaaagccaa aattttacgc
     5881 actggatatg tttccctatc catccggtca agggctacat gtagggcatc ctgaaggtta
     5941 tacggcaacg gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc
     6001 aatggggtgg gatgcattcg gtctgcctgc tgaacaatat gcgctagata caggaaacga
     6061 tccagctgag tttacccaaa aaaatatcga aacattccga cgccaaatca attctttagg
     6121 atttagttat gactggaatc gcgaagtgaa tacaactgat ccggagtatt ataaatggac
     6181 acaatggatc tttacgaagc tttatgaaaa aggtctagct tatgaagcag aagtagcggt
     6241 aaactgggta cctgaattag gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag
     6301 tgaacgtggt ggctatgatg tagtcagaaa accaatgcgc caatggatgc tgaaaatcac
     6361 tgcttatgct gatcgcttat tggatgatct agaattagtt gattggccag aaagcattaa
     6421 agaaatgcaa agaaattgga tcggtcgttc agtaggagca aatattgaat ttaaagtggc
     6481 tggtacggat aaatcctata ccgtatttac gacacgacca gatactctat tcggtgcaac
     6541 ttactcggtt cttgcacctg aactagatct tgttagagaa attacgaccc ctgaacaaaa
     6601 agctgctgta gaagcttata tcgaagagac agctaaaaaa tcagatctaa agagaacgga
     6661 tcttgcaaaa gaaaaaacag gagtatttac tggtgcttat gcaatcaatc cagttaatgg
     6721 caaggaaatc ccaatctgga tcgccgatta tgtattatct tcatacggca caggagccat
     6781 catggctgtt ccagcacatg atgaacgtga ctttgaattc gctcaaactt tcggactaga
     6841 aattcttcca gttatcgaag gtggagatgt tgaaaaagct gcttacacag aagatggtac
     6901 gcacatcaac tctgaatttc tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa
     6961 tgaatggcta gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct tacgtgactg
     7021 gctattctcc cgtcaacgtt actggggtga accaatccca atcatccatt gggaggacgg
     7081 aacagtcaca gctgtcccag aagaagagct accattacgg ttgcctaaga caaaaaacat
     7141 caagccaagc ggtactggag aatctccatt agcaaatatc gaggagtggg tcaatgtcgt
     7201 tgatcctgta acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac aatgggcagg
     7261 aagctcatgg tattacctgc gttatatcga ccctcataac aaaaaagaac tagcagatta
     7321 tgaaaagttg aagcgttggc taccagttga catctacatt ggtggagcag aacatgcggt
     7381 acttcattta ctatatgcac gattctggca taaattctta tacgatatcg gtgttgttcc
     7441 tacaaaagaa ccattccaaa aactatacaa tcaaggaatg atcttaggcg aaaacaacga
     7501 aaaaatgtca aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg aaacttacgg
     7561 tgcagataca ctgcgcatgt acgaaatgtt catggggcca ttagatgctt cgattgcttg
     7621 gagcgaaaat ggcttagaag gaagccgtaa attcttggat cgtgtatggc gtttgatcgt
     7681 tgatgagaac aataaaatgc gtgaccgtat cactacattg aatgatggca agcttgataa
     7741 agtgtatcat caaacagtga aaaaagtgac agaagactat gaaaatcttc atttcaatac
     7801 agcaatctcc caattgatgg tctttatcaa tgaagcctat aaagtggacg ctctgcctta
     7861 tgaatatatc gaaggatttg ttcaactact cgcaccgatt gctcctcaca tgggtgaaga
     7921 actatgggca atccttggaa atgatggtgg tatttcttac gcaccatggc caacatacga
     7981 tgaagcagca cttgttgaag acgaagtaga agttgttttc caagtaaacg gtaaagttcg
     8041 tgcgaaaagc aatgttcccc gagacttagg aaaagatgag ctagaaaaag cagcattagc
     8101 caatgaaata gtgcaagaat atattgaagg caaaacagtc agaaaagtta ttgcggtacc
     8161 aaacaagtta gtcaatatcg ttgctaatta attggctctt tgtcaataag gactgatgag
     8221 ttgtgcaaaa tcaaatctgg gccagaatga acttcattct gtctcagatt ttttgttatt
     8281 tcaaattgat taattgattg atcaaaaagg ttttgatttt catgttctca aacacgttca
     8341 atgtcttttt cttctgaatg gtcttgtaag agatctctgt aatcatcaaa tttttgtcta
     8401 ttaatcttag tattttttga tgtataaact acctgaaatt gagagtacat accaaattca
     8461 taaatttttt attagtggga ctagtgattg tatggatttt gacgcataat gaatgaagta
     8521 tgagaaaagg agagacaaat ggatcaagaa ctatttatta tcgaagtatt atcagctaaa
     8581 caaaaaatta tcgagaaagc gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc
     8641 ttagtatttt tctctggttt gctttttttc ggagctgtat cgttcctata tcgccatatg
     8701 agtgcagtag tccgttcagc tgaagggatg caaaaattaa ataatttgta tcacttttcg
     8761 tggggagttg gcggaataac aattgttgtg cttattgttt ttcttctact tatttggctc
     8821 cgttaccatc aattgtcgcg tcatttcgat caatggctta gtcaaagagc acaacagttt
     8881 ttacataata aaccaacttc tcaagatgat gactatcttt attttgagaa gacttcgacg
     8941 agtcaagctt ttcggataca aaaaagtgat tgccagttat tgctgagatc acttgatgat
     9001 ttacctatct acttaggcga aagcaaatca aaattcggaa tgaacacatt ccatattttt
     9061 ttactagaaa acgaacccag atgggaaaaa agccagtctt cgcctggtgg atacctgaca
     9121 actaagatga gtacattagt cactggtttt ttcctcattc ttttagctgg aacaatctat
     9181 tatgggagat acactcagcc aaaaatgtct tctcatataa atgctgcttc tcacatgaca
     9241 atgaactcaa ctaacgatac gcatatacaa aagacaaatg aagggttgtt agctgatcct
     9301 aagcaaaagg taaatttgac aacacaaaag ataaatgatc tcaaacttga tacagagacg
     9361 cactctctct atatgacaac agatagtggt gctaattggg tatttgtgcc gattgattcg
     9421 caatggttgc gttttggcga atacaccctg acaacggggc tgattcctga aggttattgg
     9481 atggacaaca catatgatat ttccgccgat ttctcatggt ttatctattc ttctggggat
     9541 gatgtacgtc tgctgacttc aagagataat ggtaaaacat ggcaaacgtc tattatcacc
     9601 caatcagttc agccgatacg ttatcgaaaa gcggtatttt atggcaacga tggtctattt
     9661 atctgtacat cagccagtgg gatgtctgca gaaactttgt acatttacac tactatggat
     9721 ggcggaatca cttggataaa aagtggaagt acaatgatca atcagccagt acaaaatgtc
     9781 agcttcgtta caacttcttt aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac
     9841 aacggaggac gctcattcaa agaatcagtt ctgtcgattc cggaaaacta tcagacaggg
     9901 gggatagatc tttttcaatc accaaatgaa gttactcaaa taagcgcgaa tacgttagag
     9961 acaaaatttt atttgttgaa aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc
    10021 ttccgttcaa ctgataacgg ggaaacatgg caattcgttc agcaactatc gcaagtagaa
    10081 caaggagatt aaagaagaga gattgtgagc ctgacgttca gcaccgagtc acaagaaaca
    10141 aattcaaaaa acagcttctc atatttgttg aatagtgtaa ctcgtgattc ggagttgaca
    10201 agagcaacaa gaagcgattt tgaagtatag cttctcgtat tttacaagat cgtgtcttat
    10261 tgccgatagg ttgtctcttg aacaccgttt attcgtaaaa agggattgtg acaagtcttt
    10321 tgtcactact taccgaacga aactgaagga gctatccaaa aagtatcatt agtattattc
    10381 tttgtgtttc tagtggcacc actagtacat ttaaaaaagc tatcgatcat ggactaatca
    10441 tacatgatcg atagcttttt agataatgtt tgttacgcta tctgaaattg actattataa
    10501 agactggcat aaagcgttgg tgattggagt agttcagaat gtgttccttt ttcgacgata
    10561 tcgccattct tcattacaag gatcatgtct gcattttcaa tcgttgacaa acggtgtgca
    10621 atcacaaaac ttgtcctatc ttttgtcaca gcttccattg cttgctgaat caatgcttct
    10681 gttcgtgtat ctacgctaga agttgcttca tctaaaataa cgacaggagg attcgctaga
    10741 atgactcgag caattgtcaa taattgttgc tgtccttgag ataacgtgcc gttttcgcta
    10801 gaaatgatcg tatcatatcc gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt
    10861 tcagccgctt cgataatttg ttctcttgtg gcatccattt tcccgtaggc gatattatca
    10921 gctaccgttc cttcaaacag ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt
    10981 tctccacgtg aaagcttggt aatatcaaga ccgtcgaatt cgatctttcc tttattcagt
    11041 tcataaaaac gcatcagcag attgactaaa gtcgtttttc ctgcaccagt tggaccaacg
    11101 atcgctaccg tttgttttgg ctttactgaa aaatcgactt gattcatcaa tattttatct
    11161 gatgtgtaac caaactgtac atttttgaaa gctatttttc ctttcggttg ttctaaaagt
    11221 cttgcatctg gaaggtctgg aatttcatct gtttcatcta atatctcaaa tatcctatcg
    11281 attgctgcca ttgcggattg gatcgagttg atcacataag aagcggtaga aattggttca
    11341 gaaatctgat tgatgtactg aagataagct tgtaagaagc cgatcgtcac accacctgat
    11401 agaacaagca tagcaccgaa taccgcactt atgatgaagg cgagttgatt aatgaaacga
    11461 atggccggat agatagcaca gttcataaat tgagcacgtt taaaagcgtt gtattgcttt
    11521 ttatttactt cgtgtatagc agcttctgca tgagcttgct gattgaatgt tttgaccact
    11581 agattaccag ctaagatttc ttccattcga ttatttaaaa cacccagttc atactgattc
    11641 tgttcagaaa tctgtttgtt tttgttcgca atcttagttg taacaattga actgccaacg
    11701 atcagcaata agacgagcaa agtaagcttg ctttcgatat aaaacagcat cagaccagca
    11761 gcagctattg ttacgacaga agtaaaaaat tgattgatac ctgtcaataa cacttgagaa
    11821 agttgattga gaccggtagt tgtccgacta atgatgtcac ctacttgatg attgtcaaaa
    11881 aacgacatcg gtaatgtttt gaattttttt gtgatttctt ttcgcaccct caatgtgatc
    11941 ttttcgctta acgctgccat tgtccgttcc tgaataaatg aagtgatgct gcttaagact
    12001 gaacagccga tcagcaataa gacagggaac aacaatgctt gtttgatatc actgaaagaa
    12061 agctgtccaa tacctgtttt tcttatttct gataagagat catcgatccc gattcccatg
    12121 atcaagggca tggctacgac taataggttc ccgaataaac tgcatagaaa caatccgtaa
    12181 aaatatttat gttcaggttt gatcattttc caaaaacgta tcaaagcacg cgtgttcaac
    12241 gtttttttct tttccatcgt atcccttctt tctatattcc ttgtgatttt gcaaactcct
    12301 gataagtttg gcaattagac agtagttctt catgagtacc ttgtccaacg atttttcctt
    12361 catctaaaac aatgatgtta tctgcttgtt ggattgtact gaggcgttga gcaacaatca
    12421 cgagagtttt ctccgacatg tgtttatgaa gggctgctct taatgctgca tctgttttat
    12481 agtctaaagc ggagaaacta tcatcgaata catagatctc tgctggtttg atcaacgctc
    12541 gagcaataca tatccgctgt ttctggcccc ctgaataatt agaaccgcct tgagcaacaa
    12601 agctgtccaa tccgtcagga agggtgtcca ctagttcttc taattgagcg atcttgatcg
    12661 ctttagacaa ctcttctgtt gaggcatatt catttcccat ctgcaagttg cttaaaattg
    12721 tcccgctgaa taagaaagcc ttctgaggga cataactgat gtgggagcgg atctgttctt
    12781 gagtcatttg agtaagagga ataccaccaa gggttacttt tcctatcgta gggtcactaa
    12841 gtctcaagag aagtttcgcc aaactacttt ttccagatcc ggtcccgcca acgatcgctg
    12901 ttgtttttcc tttaggtatt gtgaaatcaa tatcttctag aactggatca gagatttcag
    12961 tatatttaaa tgtgacatgt tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag
    13021 ctactgtttt gatcgtctct tcctcaattt cgatctctgc ttgcagtatt tcttctatcc
    13081 gtcgtaatga ggcaaacgat ttagggataa tgaccaatac catagctgcg atcatcaagt
    13141 aagcaagtgt cagcatagag tattcgacta cagcagtcac gacaccgatt tgtaatgctc
    13201 cttctgctac aagatttccg ccaaaccaca aaatagaagc ataaacgatt cccatgagca
    13261 aaaaagcaat tggagtgacc caagcaaata ttcggttcac tttgatcatt tgattagcat
    13321 agtcagcaaa gctcttattc gttcgctttt cttcatatga ttgattatca aaggcacgga
    13381 tcatattaat cccagtaaag aattctctca atgtgatggt gatccgatcc atttttggct
    13441 ggatactcaa agataaaggt gcccctcgtt tcatcagtcg ataaacaatc catacataaa
    13501 aaacaacaga gatcaatgta acccatgtta gtaatagaga ataacgaaac atcatgagaa
    13561 tggtgaaaac gcaaataacc ggtgctggta agatcatttg gcaaaataaa acaatcattt
    13621 gctggacatt atcgacatca ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac
    13681 tgatatcctt cattgacagc tgttggaatt tacggaaata tttctcccgc gtttggaatc
    13741 caaacctagc ggaagccaat gctgaaaagt aactagaggc aactgcagcg attgttccta
    13801 gaaacgctac aagcagcatt tcaactccaa ttttttttat aactgaggct tcgccacttg
    13861 ctatacctgt gtcgatcagc tttgctacta attttggtac acctaatgta ccggcgattt
    13921 ctaaacagat catcacaaag gtaaggagag cgagtccttt gtttttctgt aagaatgttt
    13981 ttaagataga catattattc ctcctttttt cctgatactt ttcgattcta taccttcaag
    14041 taacttgaag gtcaatagaa aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa
    14101 aatgtatacg attggccaag tatcggaact atttgatttg ccaaaatcaa cgatccgcta
    14161 ttgggatgaa caaggattaa tccgttcaag tagacaagaa gagaatgatt atcgattgtt
    14221 tgatatcgat gatattttta tgctttatga tatcgttttt tatagaaaat tagatatacc
    14281 gatcaaacag atgaagaatt tatatggaaa aacattgacg gagttatatg agacacttga
    14341 tgaaacagaa agaagaatcc ataaagaact ggtggtgatg aaacaaaaac aaaaagagat
    14401 aagggagcaa aaaaaacagt tgaaattgat gatcgataca aacgaggaag aatttcctgt
    14461 agaagagatc ccttttgact gtatgatctc aacggagttt gaagatattg tggaaatcaa
    14521 aaaatttcta cctaactatt caagctttgg gatgatgagt atgccagctt ccagttcaca
    14581 gacgatgtat ggatttttta tcgaccctag tgaagtacat ttatttactc aagacgtgat
    14641 ttgggagaaa aaagatacgg ctgtttatcg tcgattctta ttgaaatccg agatgaacca
    14701 tgcggaacgt aataatattt tagaaatcag agaaagaatg tatgaaaaag gatggaaaac
    14761 aggagaagtg atcggccagt atctattgac gaatacggat gaaaacaata tcagaactga
    14821 atattatcat gcatggatag aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa
    14881 aatgatggtt gttttagtta taaagaaaaa ttttctggtt cgattacggg tttaatgtgg
    14941 gggattatta gtcgtggata gatagaattt tagtttttta gaggaaatat gtctttaaaa
    15001 atggaatggt tgcaattctt agcgatatat acgatgtaat tgtcctctta aaaaagtatg
    15061 gaaaaataat tgtattgggt gttatgtata attcatggtg aactgagact aatgagaaga
    15121 aaaattcata ttgttatgag tgagattgta tcagatgaga atgataaatt aaataggtgc
    15181 ataaaagcat tacacttaaa atacattgtt gcttaca
//
LOCUS       DAFCHQ010000062        15072 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000062 DAFCHQ010000000
VERSION     DAFCHQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.062"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            78..686
                     /locus_tag="LNC71_002065"
                     /pseudo
     CDS             78..686
                     /locus_tag="LNC71_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="LNC71_002066"
     CDS             706..1734
                     /locus_tag="LNC71_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK6467113.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="LNC71_002067"
     CDS             1731..2489
                     /locus_tag="LNC71_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6467114.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="LNC71_002068"
     CDS             complement(2575..3315)
                     /locus_tag="LNC71_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6467115.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="LNC71_002069"
     CDS             3376..4629
                     /locus_tag="LNC71_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBK6467116.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4930..5178
                     /locus_tag="LNC71_002070"
                     /pseudo
     CDS             4930..5178
                     /locus_tag="LNC71_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="LNC71_002071"
     CDS             5300..5626
                     /locus_tag="LNC71_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBK6467117.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="LNC71_002072"
     CDS             5694..6407
                     /locus_tag="LNC71_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBK6467118.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /locus_tag="LNC71_002073"
                     /pseudo
     CDS             6717..7569
                     /locus_tag="LNC71_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7690..9555
                     /locus_tag="LNC71_002074"
     CDS             7690..9555
                     /locus_tag="LNC71_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6467119.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9603..11012
                     /locus_tag="LNC71_002075"
     CDS             9603..11012
                     /locus_tag="LNC71_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBK6467120.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11120..11821
                     /locus_tag="LNC71_002076"
     CDS             11120..11821
                     /locus_tag="LNC71_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6467121.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11980..12459
                     /locus_tag="LNC71_002077"
                     /pseudo
     CDS             11980..12459
                     /locus_tag="LNC71_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13307..13882
                     /locus_tag="LNC71_002078"
     CDS             13307..13882
                     /locus_tag="LNC71_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBK6467122.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="LNC71_002079"
     CDS             13882..14322
                     /locus_tag="LNC71_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBK6467123.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="LNC71_002080"
     CDS             14338..15039
                     /locus_tag="LNC71_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBK6467124.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5083 a         2501 c         3179 g         4309 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa tt
//
LOCUS       DAFCHQ010000063        14865 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000063 DAFCHQ010000000
VERSION     DAFCHQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.063"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            445..1059
                     /locus_tag="LNC71_002081"
     CDS             445..1059
                     /locus_tag="LNC71_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6467125.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1832..3571
                     /gene="yfcC"
                     /locus_tag="LNC71_002082"
     CDS             1832..3571
                     /gene="yfcC"
                     /locus_tag="LNC71_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK6467126.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3728..4912
                     /gene="iadA"
                     /locus_tag="LNC71_002083"
     CDS             3728..4912
                     /gene="iadA"
                     /locus_tag="LNC71_002083"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBK6467127.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5023..5226
                     /locus_tag="LNC71_002084"
     CDS             5023..5226
                     /locus_tag="LNC71_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBK6467128.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5327..5989)
                     /locus_tag="LNC71_002085"
     CDS             complement(5327..5989)
                     /locus_tag="LNC71_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467129.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(6002..7504)
                     /locus_tag="LNC71_002086"
     CDS             complement(6002..7504)
                     /locus_tag="LNC71_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6467130.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8052..9731
                     /locus_tag="LNC71_002087"
     CDS             8052..9731
                     /locus_tag="LNC71_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBK6467131.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9858..10337
                     /locus_tag="LNC71_002088"
     CDS             9858..10337
                     /locus_tag="LNC71_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBK6467132.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10556..11236
                     /locus_tag="LNC71_002089"
     CDS             10556..11236
                     /locus_tag="LNC71_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBK6467133.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11244..12575
                     /locus_tag="LNC71_002090"
     CDS             11244..12575
                     /locus_tag="LNC71_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6467134.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12590..13552
                     /locus_tag="LNC71_002091"
     CDS             12590..13552
                     /locus_tag="LNC71_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBK6467135.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13663..14001
                     /locus_tag="LNC71_002092"
     CDS             13663..14001
                     /locus_tag="LNC71_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBK6467136.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14080..14787
                     /locus_tag="LNC71_002093"
     CDS             14080..14787
                     /locus_tag="LNC71_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBK6467137.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4773 a         2581 c         3192 g         4319 t
ORIGIN      
        1 tttatatata tataaatcat accaatgttt ttttgtattt tcttgcttgc acttggataa
       61 gagattactt acaataaatt cagatagaaa cagctgaata aaaaaaggct ccctgaccct
      121 tagttggcgc taagaatcag ggaatgacca gtcacacaca tacaccctga ccgatcattt
      181 tgccatagtc aatgcagtgc atcgacctaa tttattgtac ctaatctagg gtgaaaattc
      241 tacccctatg tagatatttt ccatggtctg cacgttgttt tatttgctat gtcaaggtat
      301 tgggcgtatt cgttcaatgc cttttttgtg tttgaaacag caaagaccac atgtgttact
      361 gtctaactac tgttttttta tgtacttcct tagattaggc agctgtttct attcatgtca
      421 agtatagcta ggcgggagag ataaatgagt ttatgttatt ttgactatta tcaccaattg
      481 gaagagaaat taaggataga agagccgctg atgcttcaag ctggcacttg gatcgcgaaa
      541 caaattaatc aaggtggtag attgcagata tatgctagcc gcacactaag tggtgtagca
      601 tttgaatttt gggatcaaac cccggatctt gtaccaagta tattgattga aaatccagcc
      661 ggaggtgtct atgaaacgct agaaggtgca ggacaggcaa ttattgatca gctttctgtc
      721 aaatcagaag acatttttct tcttctttcg aatgaaggaa gaaacccggc aatcatcgaa
      781 ctagctcagt ggatcaaaga aaatggtcat ccattaatca ttgttactgg atttgatttg
      841 tcacgaagta tcaaatcgca acatagcagt ggtttgcgat tatttgaatt tgctgatttg
      901 gttttggaca accacgccaa tttacatgat gcagtcttga ctttgccaaa tctagatcca
      961 gcgatctgcg gaacagcctc tcttgcaacg atctttttgc tgcagcaagt tttgtatttt
     1021 acagcaaaac agctagtcca ggacacaagt ggaaaatagt tatccacatg ttgacaaagt
     1081 tccttaaaaa aataatgttc cacataaaaa tggagcgttt gaaaaaaaga aaacgattat
     1141 taatgaggat taacaacgac tacttttttt gttttagtta ttttaagatg gatcatcttt
     1201 gattttaagc ttgttttgtt taaagtttgt tatttttata agttgtttca cagttatgag
     1261 aaatgagtta taagaatatg tagtgtcaga aaaataaaaa gtagtggaaa aagagacaag
     1321 aaagaatcca tatggattct ttcttgtctc ttaatacacg ttgatgaaag agtcttcccc
     1381 aaaactcttt acaaagtaat tatagtacat ttgttatcat tattgcaatg aagtttacct
     1441 gatataacaa aattgtccaa caggatacct ttttctaata gaaataaaat acacaaaaat
     1501 tgaaggatag ataatggata tttataagtg tttattattt tttttgcaat gaaattcaat
     1561 ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt tctctaatct aaaaataaaa
     1621 aaagaatttt aaggaaaaac agagaaataa aatacttaat ataaatggaa agagaaaaaa
     1681 ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag tgattttcta ataaattatt
     1741 catgtggaaa tgtcattttt actgtataat tcatttgatt atccattttt tattcatcaa
     1801 aattactgaa aaaagaaagg aagaaataaa tttgaagaaa acaaattctt caggaaaacc
     1861 gaaacgcgac ttcactaaat tatcgacacc ccatacgtat gtcattattt ttggtgtagt
     1921 catctttgca tggatactga cttttgtcgt tccagcagga aagttcagca cgcaagatat
     1981 tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact gttttacgtc aagattcctt
     2041 ccgttatgct tacgagctgg ataagtcgta tgtgtttgat caattagaag aactgcaaga
     2101 tcatccagca gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa aagtcattgc
     2161 tgacggagaa aaaaatctta cgcaggaaaa actagatgaa atctccttga cagatgatgt
     2221 gctttacgat cagtacggcg aaaatatcta tgatacttct aaaaaattgc ataaaacagc
     2281 caaaatatgg ggaacagatg attttggcgg ctttggtttc ttaaattttg tttttgaagg
     2341 actagtatct ggagataagt atggatctgc tgtagggatc gctgcattga tccttgttgt
     2401 tggaggtgct tttggtatta ttatgcgtac cggagcgatc gacgcaggta tctatgcgtt
     2461 tatcagtaaa actaagggac tggagcgttt agctttaccg ctattattct ttgctttttc
     2521 attcggtggc gctacattcg ggatggcaga agaggtaatt ccattctcaa tggtgatggt
     2581 tccatttgtc attgcgttag gatatgactc gatcgtagca gtaaccgtga cttatgtcgc
     2641 atctcaagtc ggaaatgcga cctcttggat gagcccgttt agcgtagcgg tggctcaagg
     2701 gattgctgga atccccgtgt tgtctggtgc gacattccga ttgattatgt gggtagttgt
     2761 tactgcatta gccgctggtt atatgatgat atatgcagaa aaaatccgta aaaaaccaga
     2821 aagttcgttg acttacaaat cagatgatta tttccgttct catatcaaaa agacatctga
     2881 tgaaaataaa aaattcatgc ttgggcataa attgatctta ttagagatgt tagttgtcct
     2941 tgtctggatc gtttggggcg tgactcaaaa aggctattac attccagaaa tcgcttctca
     3001 attctttgtg atgggattag ttgctggaat cattgctgtt cttttcaaat tagatggaat
     3061 gaaagtcaat gacatcgctt cttctttcca atctggtgca gctgatttag ctggtacagc
     3121 aatcgtagta ggtatggcca aagggattct tcttgtcttg ggaggatctg atgcatctgt
     3181 tccttcagct ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg gtgttccagc
     3241 tgtcattggt gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg ttgttacatc
     3301 aaattcagga caagcagctt tgactatgcc aatcatggct ccactggcag atttagtagg
     3361 tgtatccaga caagtagcag ttcttgctta tcagcttggt tcaggtttta tggacgcttt
     3421 tacacccgta tcagccagct tgattggtgt gctaggtgtt gcacggatcg aatgggcaaa
     3481 atgggcaaga ttccaaatca agatgcaagg attcctgttt gttttaggaa ctattttcat
     3541 catgattgcc attgcaatag gtctgcagta gtactggaga cctgtaaata aaaaagtaat
     3601 ccaaacagta gataaggact ccaagaaccg tcattttacg gtaaaatcaa aggagtaaag
     3661 atcctatcgt ttggattatt ttttaagatc ctatcatcag ttatacttaa agaaagagga
     3721 aaaatgtatg aaaatcattc gacaaattga agtgtttgca ccagattact taggaaaaat
     3781 ggatgtactg attgccggtg ggaagatttt ggctgtagaa gaacagctag aaggcggcta
     3841 cgaaggagtg gaagtagaag aactttcagg tgagggaaaa gtactaaccc cgggtttcat
     3901 tgactgtcat ttccatattt taggtggagg aggagaaggc ggttatcaaa atcgaacgcc
     3961 agaagtcaca ttgagccaat tgactacagc tggtgtaacg acagttgtcg gttgtttggg
     4021 aacagacgga gaaggtagag atatgactgc gttgatcagc aaagcgaaag gtctggaagc
     4081 agaaggaatc tccacttatg tatacgaagg ttcgtaccga ttgccggtaa aaacagtaac
     4141 agattctatc atcaaagact ttttgacaat tgataaaatt atcggaatcg gagaaatcgc
     4201 agtatccgat caccgttctt ctcagccaag ttttgaagag ttcgctcgtg cagtagcaga
     4261 tgctcgtgta ggaggcatgt tatccggaaa agctgggatc atcaatgtcc acttaggcgg
     4321 aggaaaaaga aagctagagc tattgacacg aatcgtagaa gaaacagaaa ttccgatcac
     4381 acaatttttg cctactcatg caaatcgcac accagaatta tttgaagctt gcgtggcatt
     4441 tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa gatcctgatt tttgggagaa
     4501 gacagatggt gaagttcgct tcagcaaagc actgaaacga ttgatcgagg aagatgtttc
     4561 tttagataat ttcacgatga cctcagatgg acaaggaagc ttgccttatt ttgatgaaaa
     4621 caatcatttc ctagggttag gagtaggaag tgccaaggca ttgctagtag ggatcaaaga
     4681 agctgtgcaa aaagaaagta tccctttgga aatcgcctta cgagctataa ccagtaatcc
     4741 agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg atgcagatct
     4801 gtgtatcttg gataaagaaa cgcttgatat tgatacagtt atcgctaaag gagaaataat
     4861 ggtccaagaa aaagaagtca aagtatgggg gactttcgaa aaaagttttt aatcaacagc
     4921 tctaactatc ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg ttgatgttac
     4981 cctgaaggta gatcaataaa caaactaaga ggaggaagca caatggcaga tttaaaagga
     5041 cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa aagaagcata cggcaaagca
     5101 acagacgaca agagcaaaga aacagaaggg aaagcacaat cactaggtgc tgacatcaaa
     5161 gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa agacgaaaaa
     5221 aaataaaaac cacttattac taattgaatc aagcaaataa aaaatccgaa ccatatctgc
     5281 gagacagata tggttcggat tttttatttg atcattatcc tgttttttaa acttcgattt
     5341 tttgaaattt tttatttttt agttcaaaga cttcatcaca gctttttgct acttcaatct
     5401 catgagtcac gatgataata cagcgatttt gttcatgggc gatttcttgg aaaagtttga
     5461 cgatatcttg tgaagttgtt tcatctagat ttccagtagg ctcatccgct acgattagtt
     5521 gatgttcaca gcagatagca cgaacgatag cgactcgctg ctgctgacct cccgaaagtt
     5581 tggtcacctg tttttttgct aatatttcat caattcctac acgagccagt cgatctaaag
     5641 caacttgtcg ataattctct tgcttggtct tagtgatttc cattgctgct atgacgttgt
     5701 ctagagccga taaataagga agcaagttgt aagcttggaa aataatcgag acatcatttt
     5761 tacgataact tcttaacccg attttgttca gtgaacgatc ttgatagtat acctttcctt
     5821 cttttggtgc atctagtcca gaaataagcg aaagaaaagt cgtttttcca gaaccacttg
     5881 ctcctaaaat agcgtacatt tttccagctt cgaaattcag ttctacatct ttataaagcg
     5941 catcttgcgg atcttgatac caaaatccca attgttctgt ccgtaacatc aaattcctcc
     6001 tttagtttaa taggattttt cttgggttta atcttaatat ccctatagaa gctaaaaaga
     6061 cagaaaacag actgattcca aatgctactc cggctaatag tcccaattgt gcaggtttga
     6121 cattgatttc caattcgctg acttgatcag aaacagcaaa tggattattc gtggttcctt
     6181 gttgaccatc gcgttgaccg ccttgttctt gtctgttacc tggcatttgt ccaccgccag
     6241 gagcttgctg gttttgtgtc gtggtttctg tctgttggtc aagtagctgc tgtcccacgg
     6301 cattcgcgac gacgtttccg ctaagcgaag caactcctaa tgcaagaatc atacaaatag
     6361 ccacttcggt aaacaattgg agaatgattt tacttctgga ttctcctaaa gatagcaaga
     6421 cgcctaactc atgccgacgc tcccgaatgg taatcataat gatcagagta agtatgataa
     6481 taccggctac agccactagt aaaacaacat ttttagaaaa acttgccacg ttgtttaaag
     6541 gttccaacat tgactgatac atggaatcat ttgattgtaa gctaaatgta tctgtgtcga
     6601 tcagcttctc agcttctttg agaaactcat ccattttatc tggatcagat agagtgtaaa
     6661 tagcagaatc tatcgtgtta tcgtcagatg tcccatttag ttcatttgca aaagtgtaag
     6721 aagtgtaaag tgtattcgac ggattcatga agttgaattt catacccata ctgccgcttg
     6781 tttctctgga ttcataaatt cctttgatcg tcatttcata agtggtatct tcatcttcag
     6841 tactcgtgat tgtgaaagta tctccgactg ccaggtcatt tgtttctgct agagtagaat
     6901 caatgactac attgtttgtg cctttgtcgc tgtcatctat tccttcacca tccgtgattt
     6961 ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc tgacacacca ctgatttgaa
     7021 agtctgcttg agtcatttgc ggattcccgc ccattcctcc agcaggttca ccgcctgcat
     7081 cagtagtttc tgaactgtct tcacttgaat cagaactagt gatggcagtt attccatctt
     7141 ttgccatagc tgaggcggaa ctttcaaagg aataactctt tacattatct aattcagcta
     7201 ttttttccgc gtccgttaag gaaactggtg taagagaaaa actgcctgga tctggacgtt
     7261 cttcagtggt agaaccgctt gaagaagttt gttcctgttt tttaaatgaa gcttctcgat
     7321 tggcacttaa agtcactgtt gctccaactt ctttttgtgc ttgattcgct gcttcttctg
     7381 cagcgcttcg aatcgtgagg ccagctagca caaaaataag aatggctgtg aacacggcaa
     7441 acaataacaa tgaacgtccc cattttactt ttgtactttt ccatgcacgt ttcataaaat
     7501 tcatattttg catccctcct agtagatgca tttgataata gacaacctat cttaaagcta
     7561 gcttaaatct tatgctgttt gcacaccatc aacatgaggt tctgtatata taaaaagtca
     7621 ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac cgacgaagaa
     7681 gaaatgaaag aggaatagca aaagaaatcc tagatgctac agagggattt ttacgtgcat
     7741 agttgttcta acaattgctg atactagcga tttagtttat ttgttgtata ataatgggga
     7801 acagctgaat acttatataa gtattctggc tatagcgttt cgcaaaagat ccattttgtt
     7861 aatgaagtat atccaaaata aaatgcccat ccaactgtca gaacgcggat gtgctggtaa
     7921 ttttcctgct gattctccat aagttgagaa atagtgattt tgcatatgct tcattggaat
     7981 ggctcatata tttagataaa gaagaaaatc gacatcttta aacttggatg tcataagaag
     8041 aggtgaagtt tgtgagtacc attaaaatta tcccattagg cggcgttcgt gaaaatggaa
     8101 aaaacatgta tgtcgcagaa gtgggcgagg atatttttat tttagattgt gggctaaaat
     8161 atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc tgattttacg tatttagaag
     8221 aaaacattga tcgtgtagct ggtgtctttt taacacatgg acatgcagat gcgatcgggg
     8281 cacttcctta cttgttgtca aaaatttctg taccagtatt cgggacagag ttgacgatcg
     8341 agttagcaaa attgaatgtc agtcgtaatg aagcagccaa aggatttaag gatttccatg
     8401 tggtagatga gcatacagaa atagactttg gtgaaacagt agtgagcttt ttccgtacca
     8461 ctcatacgat accagattca gtggggatca atctgaaaac aaaagaaggt agtatcgttt
     8521 atacgggaga ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca gattatgcac
     8581 gattggcgga aatcggcaaa gaaggagtct tagccttact aagtgaatcg gcaaatgcag
     8641 aaaatcctca gcctgtagct tctgaagccc aaatagcaga tgaagtattt gatacgatca
     8701 aatattggga agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa cgagtgcaac
     8761 aagtattgga tgcagcagaa aaagcacaac gaaaagttgt cttgactggt caagatttcg
     8821 aacgaattat tcgcacagcg atgcgtttag aaaaacttca attaccaagt gaagatctgc
     8881 ttgttacgtt gaaagacatg aaaaactatg atccagaaga actgatcatt ttagaaactg
     8941 gccgaatggg tgaacccatc aagtctctgc aaaaaatggc aaacggtaca caccgtaatg
     9001 tcaagattga agaaggagat ttggtttata tcactactac gccaagcatc gcgatggaaa
     9061 ccatcgtggc gaaaacggaa gatatcattt atcgagctgg tggaacagtg aagttgatct
     9121 ctgagaatat gcgcgtttct ggccatgcca atcctaatga cttgcaatta atgatcaatt
     9181 tgatgaaacc aacatatttt gtgccagttc aaggagagta tcgtgaatta gctgctcatg
     9241 cagatttggc tcatgcagta gggatgccat acaaaaatat ctatattact ggacgcggag
     9301 acgtgctgga atataagaac aagcgtatga gtgttgcagg aactacaagt gcagaaaatg
     9361 taatgattga tggattaggc ataggtgata tcggaaacat tgttttacgt gatcgaaaaa
     9421 tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat cagtcggcga gaaaagaaaa
     9481 tcatttcaaa acctcaaatt acttcacggg gattcgttta cgtaaaagct agccgtgatc
     9541 tgatgaaaga aagcagcaat atgattgaaa caatcgttga gaaacattta gaaagcaatg
     9601 attttgagtg gagcaaatta aagcaagaca tccgagatca attaggccgt tacttatttg
     9661 aacaaacaaa acgccgccca gtgatcttgc cagtcattat ggaagctact caacgtcgaa
     9721 ataaaaaata aacaaaaaga gtaagatgaa cctctctata gagtgctcgt cttgctcttt
     9781 ttttgcgaaa aacaaataat ttttgaatac agcatccctc gctttataat aaacgataga
     9841 ataaagggag tgaatcaatg atgagtcaaa caaaagtcga acgttattta gaagaacaaa
     9901 agatcagcta tcaaccactt gattttagtc atgtggtaga agaatctttc acagaagaac
     9961 tacaaaaaag agggattgat ccagctttga tatgtaaaac tctcgtagtg aaaggaaaca
    10021 aaactggggt gatcatcgca ttgattcctt tgaacgatca cttagattac aaaaaaacga
    10081 gacaagtgac gaaagatcgt aaagttggtt ttcctggaat ggattttgta ttaatgcata
    10141 caggatatcc ccacggagca aacaccccta ttggaataaa actcgcacat cctgattatt
    10201 gttttttagc agacagttct ttaaaagaaa aagaagaagt aattgtgtca agcggtgaaa
    10261 tcgggagaag ccttcggata aaagtagccg atttagaaaa gatcgtccat cctatttacg
    10321 ctgatttaat tagttaaggg acgagaagag aagctaagca gagcagagac ctcaaacttg
    10381 agattatatc tgcttggtac ttggtgcatc ctgtattatc tgttacaatt gctttgtaca
    10441 tactgtacta tcaatccaat gaaaatatat ttagcagcta tattttttca ttgatgtgca
    10501 agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt aaaatatgaa
    10561 aattacgtat catggacatt cctgtttaac aattgaaatg gaaaatggcc aacgcgtgat
    10621 gattgacccg tttattacag gaaatccatt gacagactta actgcagaag aagtaaagac
    10681 agattggttg ctgattactc atggtcatag tgaccacata ggagatatga ttcctattgc
    10741 aaaagaaaat caagccacaa ttatcagtat tgttgagatt gctgattatg cgaacagcag
    10801 aggagtagat tcattcggta tgaatatcgg tggaaaacat gcgtttccat tcggcgtggt
    10861 aaaattcgta catgcacagc atagttcaag ctatgaagta gacggtatcg ttcagtatat
    10921 gggcgagcct agcggaatta ttattcaagc agaaggaaaa acgatttatc atgcagggga
    10981 tactgcttat ttcagtgatc taggattgct tgcagaagaa ttcgatattg atgtcgcttt
    11041 cttgccgatt ggcgataact atacgatggg gccagaagat gcagcaaagg ctgcgcaaac
    11101 aattcgtgcc aagaaagtag ttccaatcca ttacaatact ttccctgtca ttgaacaaga
    11161 tcccgaatct tttgtagagt tactggaaga tagtgaagga aaaataatga gcgtctcgga
    11221 aacgctggaa gtgtaggcag aaaatggcga caaaacatga tttgatttta gaatatattg
    11281 aaagtttacc tgttggcaat cgaatttctg taagaagcat cgcaaaagaa ttgaatgtca
    11341 gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta gtttcaacaa
    11401 ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa aaaacatatt gaacgtttga
    11461 catttggcga ggtcgtcagg atcattgaag gggacgtttt aggcggggcg gcaggattag
    11521 acaaggtctt gaataaattc gtcatcggcg cgatgactaa acatgccatg gaacgctata
    11581 ttacgccagg atcactaatg atcgtcggca accgggcgga agtccaaaaa ttagcattgg
    11641 aagatggcgc cgcagtattg atcacaggag gattcaatac gactgctgag attgcaaagc
    11701 tagctgatga tcttgaaatg ccgatcttac gcacctctta tgatacattt acagtcgcaa
    11761 ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg cttgtcagtg
    11821 atatctatat gccagctgaa aagactcatt atttgcagca gacagatacg atttctgatt
    11881 atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac cgccatcatc
    11941 gtttgatggg gattgttacg gcaaaagatg ttttagggaa atcgccaaac cagatcatcg
    12001 atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt gcctctgtta
    12061 gccatcaaat gatctgggat gggctagaag tcatgccggt cgtttccgat gacttgacat
    12121 tggaaggctt gattacccgg caagatgtaa tgaaagcgat gcagcttgtc cagcgacaac
    12181 cgcagattgc agatacgatt tcggatcaga tatctggaaa tatccatacg attgatactg
    12241 accgtgaagg aaatcgtttg gaaaacccta agttcaaatt cgtagtagca ccacaaatgg
    12301 tcaatggcgt tggaaccatt tcttttggcg tacttagcga aatcatttct gatgtcgcac
    12361 aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg aacctacatt
    12421 atttgcggct gatccaattg gaaagcgaat tagacattcg tccacgtgta ttggaaatcg
    12481 gcagacgttc cgctaaactg gatatcgaag tgtttattga aaacaccatc gtttccaaag
    12541 caatcgttgt ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa tgacagtaca
    12601 gcaagaaata gtagatacaa taaaaaaata cgataaaatc attatccaca ggcatttgcg
    12661 ccctgatcct gatgcattag gatcacaagt aggattagcc gaactattac gggaaagttt
    12721 cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa gggcttcgct ttttagcaga
    12781 aatgcaagaa gttgaagatg tcttctacca aggagcgcta gtcatcgtga ctgatacagc
    12841 taattcaccc agaatcagtg atcaaagata tcagttggca gaaaaactga tcaaaatcga
    12901 ccatcatccc aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa aagcaagtag
    12961 ctgtagtgag atgattgcag aatttgcttt gatgtttcct gaatctttaa agatgaacgt
    13021 taaggctgca cggttacttt atgcaggaat cgtcggagat acagggcgct ttttgtatcc
    13081 ctccacgacg tcccgtacat tagagattgc ttcgatattg agaaattatc catttgatgc
    13141 aagtgctttg aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac tttccggtta
    13201 tctttatcaa aatatccagg tggatgaaaa tggtgcagga agaataatcc ttccgcaaga
    13261 aattcttgat aaatatggaa tcgatgattc agagacagct tcgatcgttt cacttcctgg
    13321 agtgattgac gaagtgctgg cttgggggat ttttgttcag cagccttcag ggtattaccg
    13381 cgtacgttta cgttctaagg ggccgatcat caatgaattg gctaaacgcc atcatggagg
    13441 aggccatcca ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag aggaaatcta
    13501 taaagagatc caagatatat gccaatcgta taagagcaga aaaacagaat gatacttcgt
    13561 tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc ttttcaagat cttctttttc
    13621 cgttacaata gtgctcacta aagattttaa aggagaaaga agatgacatt accaaattgc
    13681 ccagaatgtg gctcagagta cacatatgaa gatcgaggct tgttgatttg cccagaatgc
    13741 gggaacgaat ggaacaaaga agaacaagaa actgtcatag aagacggatt agtggtatta
    13801 gatgcaaatg gaaaccgtct gttagagggc gatagcgtga cagtgattaa agacttgaaa
    13861 gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat ccgtttagta
    13921 gaaggcgatc ataatatcga ttgcaaggta gaaggatttg gaccgatgaa actgaaatcg
    13981 gaatttgtga aaaagaatta aagaatattc aaggtctttt cacattcttt tcctactact
    14041 aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta tggaatgtat
    14101 ggttttttag atccgacatt tcttttggtc attcttggat tagttatttc cggaattgcg
    14161 tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa aaacaatgtg
    14221 actggtacac aagcagcaca atatatatta caaagtcaac agataaataa tgtcggtgtt
    14281 caacaaattg ccggagatct cacagataac tacaattcag gtaataagat gttgagtttg
    14341 tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc tcatgaatgc
    14401 ggacatgcgg tacaagatgc ttcgggttat gtcccattga agattcgtgc agcacttgtt
    14461 ccagcagcta actttggttc gatgatttcg ttccccttga ttatggtagg tgtattgctt
    14521 agctggaact ctacgttaat caatatcggg attttcgctt tttctttagc attacttttc
    14581 caattggtga cattaccagt cgaatttaac gcgtctcggc gagcaatcca aattttgagt
    14641 gaaggcggac tactgacgga agaagaagta ccgatggcaa gacacgtatt atttgcagca
    14701 gcactgactt atgttgcagc agcgctttcg acattcctgc agctgttgcg cttgatctta
    14761 ttgttcggcg gcaaccgaag agattaaaat aaagaagttg tgaaatcagc gctttgatct
    14821 gagctaatac cgagagttag ctgattgaat accatttatt tggaa
//
LOCUS       DAFCHQ010000064        14625 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000064 DAFCHQ010000000
VERSION     DAFCHQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.064"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..816
                     /locus_tag="LNC71_002094"
     CDS             4..816
                     /locus_tag="LNC71_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBK6467138.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LNC71_002095"
     CDS             813..1613
                     /locus_tag="LNC71_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBK6467139.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LNC71_002096"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LNC71_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBK6467140.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LNC71_002097"
     CDS             3110..4819
                     /locus_tag="LNC71_002097"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBK6467141.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="LNC71_002098"
     CDS             5031..9389
                     /locus_tag="LNC71_002098"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBK6467142.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="LNC71_002099"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="LNC71_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBK6467143.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="LNC71_002100"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="LNC71_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBK6467144.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="LNC71_002101"
     CDS             11227..11520
                     /locus_tag="LNC71_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBK6467145.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="LNC71_002102"
     CDS             11517..11828
                     /locus_tag="LNC71_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBK6467146.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="LNC71_002103"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="LNC71_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBK6467147.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="LNC71_002104"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="LNC71_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBK6467148.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DAFCHQ010000065        13977 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000065 DAFCHQ010000000
VERSION     DAFCHQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.065"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..460
                     /locus_tag="LNC71_002105"
     CDS             140..460
                     /locus_tag="LNC71_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467149.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="LNC71_002106"
     CDS             912..1112
                     /locus_tag="LNC71_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6467150.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="LNC71_002107"
     CDS             1259..4048
                     /locus_tag="LNC71_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK6467151.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="LNC71_002108"
     CDS             4095..4622
                     /locus_tag="LNC71_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBK6467152.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="LNC71_002109"
     CDS             4643..5833
                     /locus_tag="LNC71_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6467153.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="LNC71_002110"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="LNC71_002110"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBK6467154.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="LNC71_002111"
     CDS             7966..8640
                     /locus_tag="LNC71_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBK6467155.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="LNC71_002112"
     CDS             8637..8978
                     /locus_tag="LNC71_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467156.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="LNC71_002113"
     CDS             9008..9367
                     /locus_tag="LNC71_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBK6467157.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="LNC71_002114"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="LNC71_002114"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBK6467158.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="LNC71_002115"
     CDS             12286..13752
                     /locus_tag="LNC71_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6467159.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3938 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgtttt
//
LOCUS       DAFCHQ010000066        13959 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000066 DAFCHQ010000000
VERSION     DAFCHQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.066"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..135
                     /locus_tag="LNC71_002116"
     CDS             <1..135
                     /locus_tag="LNC71_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6467160.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="LNC71_002117"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="LNC71_002117"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBK6467161.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="LNC71_002118"
     CDS             complement(2412..2663)
                     /locus_tag="LNC71_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBK6467162.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="LNC71_002119"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="LNC71_002119"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBK6467163.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="LNC71_002120"
     CDS             3613..3831
                     /locus_tag="LNC71_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467164.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="LNC71_002121"
     CDS             complement(4120..4632)
                     /locus_tag="LNC71_002121"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBK6467165.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="LNC71_002122"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="LNC71_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBK6467166.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7528)
                     /locus_tag="LNC71_002123"
     CDS             complement(7085..7528)
                     /locus_tag="LNC71_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBK6467167.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="LNC71_002124"
     CDS             complement(7529..8317)
                     /locus_tag="LNC71_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6467168.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="LNC71_002125"
     CDS             complement(8314..9255)
                     /locus_tag="LNC71_002125"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBK6467169.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="LNC71_002126"
     CDS             complement(9432..10019)
                     /locus_tag="LNC71_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBK6467170.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="LNC71_002127"
     CDS             complement(10240..11547)
                     /gene="obgE"
                     /locus_tag="LNC71_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBK6467171.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="LNC71_002128"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="LNC71_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBK6467172.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="LNC71_002129"
     CDS             complement(12936..13871)
                     /locus_tag="LNC71_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6467173.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAFCHQ010000067        13917 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000067 DAFCHQ010000000
VERSION     DAFCHQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.067"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="LNC71_002130"
     CDS             131..505
                     /locus_tag="LNC71_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6467174.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LNC71_002131"
     CDS             complement(619..1248)
                     /locus_tag="LNC71_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6467175.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LNC71_002132"
     tRNA            complement(1382..1454)
                     /locus_tag="LNC71_002132"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LNC71_002133"
     tRNA            1668..1741
                     /locus_tag="LNC71_002133"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LNC71_002134"
     CDS             complement(1879..2043)
                     /locus_tag="LNC71_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467176.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LNC71_002135"
     CDS             2338..3666
                     /locus_tag="LNC71_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBK6467177.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LNC71_002136"
     CDS             3868..4701
                     /locus_tag="LNC71_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBK6467178.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LNC71_002137"
     CDS             4717..5454
                     /locus_tag="LNC71_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBK6467179.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LNC71_002138"
     CDS             complement(5609..6577)
                     /locus_tag="LNC71_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBK6467180.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LNC71_002139"
     CDS             6809..7642
                     /locus_tag="LNC71_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBK6467181.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LNC71_002140"
     CDS             7676..8515
                     /locus_tag="LNC71_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6467182.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LNC71_002141"
     CDS             8541..9101
                     /locus_tag="LNC71_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6467183.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LNC71_002142"
     CDS             9300..11021
                     /locus_tag="LNC71_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBK6467184.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LNC71_002143"
     CDS             11186..12328
                     /locus_tag="LNC71_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBK6467185.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LNC71_002144"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LNC71_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBK6467186.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAFCHQ010000068        12910 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000068 DAFCHQ010000000
VERSION     DAFCHQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.068"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..3660
                     /locus_tag="LNC71_002145"
     CDS             133..3660
                     /locus_tag="LNC71_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBK6467187.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="LNC71_002146"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="LNC71_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBK6467188.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="LNC71_002147"
     CDS             7448..7789
                     /locus_tag="LNC71_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467189.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="LNC71_002148"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="LNC71_002148"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBK6467190.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="LNC71_002149"
     CDS             9146..11566
                     /locus_tag="LNC71_002149"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBK6467191.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LNC71_002150"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LNC71_002150"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBK6467192.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4385 a         2243 c         2739 g         3543 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac
//
LOCUS       DAFCHQ010000069        12885 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000069 DAFCHQ010000000
VERSION     DAFCHQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.069"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            149..2314
                     /gene="acm"
                     /locus_tag="LNC71_002151"
     CDS             149..2314
                     /gene="acm"
                     /locus_tag="LNC71_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBK6467193.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2586..3071)
                     /locus_tag="LNC71_002152"
     CDS             complement(2586..3071)
                     /locus_tag="LNC71_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBK6467194.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3161..3506
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3551..4192
                     /locus_tag="LNC71_002153"
     CDS             3551..4192
                     /locus_tag="LNC71_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6467195.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4211..4846
                     /locus_tag="LNC71_002154"
     CDS             4211..4846
                     /locus_tag="LNC71_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6467196.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4864..5679
                     /locus_tag="LNC71_002155"
     CDS             4864..5679
                     /locus_tag="LNC71_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6467197.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5998..7434
                     /locus_tag="LNC71_002156"
     CDS             5998..7434
                     /locus_tag="LNC71_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6467198.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7623..10070
                     /locus_tag="LNC71_002157"
     CDS             7623..10070
                     /locus_tag="LNC71_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBK6467199.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10300..11367
                     /gene="rlmN"
                     /locus_tag="LNC71_002158"
     CDS             10300..11367
                     /gene="rlmN"
                     /locus_tag="LNC71_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBK6467200.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11560..12663)
                     /locus_tag="LNC71_002159"
     CDS             complement(11560..12663)
                     /locus_tag="LNC71_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6467201.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4407 a         2154 c         2739 g         3585 t
ORIGIN      
        1 catatttatt ttttataaat atttgtaaaa taactatcaa ttttttagtt aaaagattgg
       61 aaatgagtgg gggaggaatt gaagaaagga tttttgagag atgatatagt aggttttatt
      121 tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat
      181 gttatttatt attactaata taacatcact gattcctgtt catgtctatg cggatgcagg
      241 cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg
      301 aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga
      361 ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga
      421 aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc
      481 gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt
      541 tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt
      601 gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc
      661 tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg
      721 gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga
      781 aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg
      841 tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag
      901 cgctacattt aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt
      961 tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat
     1021 aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg
     1081 agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt
     1141 tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt
     1201 gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt
     1261 gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt
     1321 aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa
     1381 attctccata aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa
     1441 ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat
     1501 ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac
     1561 aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga
     1621 tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt
     1681 cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt
     1741 catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc
     1801 gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag
     1861 tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga
     1921 aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta
     1981 taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag gtagccatat
     2041 aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc
     2101 tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat
     2161 tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac
     2221 aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc
     2281 tgggatattc ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttgttaaat
     2341 atgactgaaa tcaaaagaat aaaattccga tttataatcg aaaaaagaag catttttata
     2401 tatgcttctt ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc
     2461 gtattagttt tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct
     2521 gatatatttt gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc
     2581 tatcattaca aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag
     2641 tcggaaaaat ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct
     2701 tgacgatcaa tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat
     2761 gctaatggag tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa
     2821 acaaaagata agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt
     2881 tcatcaggca agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt
     2941 tttacttgcg gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga
     3001 tcgacttttt gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt
     3061 gcacgactca tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac
     3121 aaatgaaaaa taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag
     3181 agtagccgtt aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg
     3241 tgaaacacat cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt
     3301 tacagctgcg gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta
     3361 aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt
     3421 ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt
     3481 ggaaccacgt ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga
     3541 ggaaaagaag atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac
     3601 aacattgaaa ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc
     3661 attaggattg tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt
     3721 tatgcgggga acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat
     3781 tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc
     3841 ttattttgca gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc
     3901 agcgcaagtt ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt
     3961 gacaaagatt gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc
     4021 cttgatctat atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg
     4081 cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat
     4141 tctaacaatc ataatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg
     4201 aggaagaaat atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat
     4261 caaagatttg aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg
     4321 tggcggtaaa accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga
     4381 attttattta gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt
     4441 aggagtcgta ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac
     4501 cttagcacca aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga
     4561 attagcggct tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg
     4621 cggacaaaaa cagcgggtag ctattgccag agcattggct atgaatccac gggtattggc
     4681 ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat
     4741 cctatcattg aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc
     4801 agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata
     4861 gagatgaaaa aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc
     4921 tgttcgaaca aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc
     4981 gttataggtt tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc
     5041 acaggatttg atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac
     5101 tttcagccca ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg
     5161 atatggaacg gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct
     5221 tatatggaaa acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga
     5281 caaatgaata ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg
     5341 aaacaaccag aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt
     5401 tttaatgaag cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa
     5461 gtatacgcag actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa
     5521 ggttcttatg agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg
     5581 aaaaaaatca atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc
     5641 gataaatggt ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa
     5701 agacgtgtca cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact
     5761 cggagctgaa tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg
     5821 gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta
     5881 ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg
     5941 ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg
     6001 gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg
     6061 tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt
     6121 gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat
     6181 catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc
     6241 tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc
     6301 gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact
     6361 ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt
     6421 cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa
     6481 gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta
     6541 gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct
     6601 aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc
     6661 ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg
     6721 gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga
     6781 aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa
     6841 gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat
     6901 caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg
     6961 acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag
     7021 aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc
     7081 ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta
     7141 ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac
     7201 ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc
     7261 tattgcacct ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat
     7321 ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg atataagaaa
     7381 gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct ttagttcaca
     7441 tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta
     7501 gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa
     7561 caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat
     7621 aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc
     7681 aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg
     7741 ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga
     7801 tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga
     7861 ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac
     7921 tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg
     7981 gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc
     8041 aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact
     8101 tcttgattgc acagcctgga agttatttag cagcagtgct attactggca ggaggagcat
     8161 tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac
     8221 gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg
     8281 aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag
     8341 ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa
     8401 atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc
     8461 caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata
     8521 caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta
     8581 cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag
     8641 ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa
     8701 aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca
     8761 aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag
     8821 taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc
     8881 ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga
     8941 tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac
     9001 tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga
     9061 tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga
     9121 ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc
     9181 cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga
     9241 cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat
     9301 atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac
     9361 agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag
     9421 atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt
     9481 tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg
     9541 tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat
     9601 caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc
     9661 gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat
     9721 tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc
     9781 attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc
     9841 cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta
     9901 tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg
     9961 aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca
    10021 ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg
    10081 atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa
    10141 aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg
    10201 agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat
    10261 agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat
    10321 ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga
    10381 gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg
    10441 aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa
    10501 cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat
    10561 aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg
    10621 acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac
    10681 cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat
    10741 gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt
    10801 gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca
    10861 attggtgctc ggcacattac agtttccact agtggattag cacacaaaat caaggaattc
    10921 gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg
    10981 cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg
    11041 gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa
    11101 gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa
    11161 ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc
    11221 agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt
    11281 gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag
    11341 caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa
    11401 aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc
    11461 gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa
    11521 tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc
    11581 ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt
    11641 ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa
    11701 gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg
    11761 gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa
    11821 taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag
    11881 aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa
    11941 aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga
    12001 gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat
    12061 gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc
    12121 agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt
    12181 atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg
    12241 aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg
    12301 cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat
    12361 gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg
    12421 gacactttca tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca
    12481 gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt
    12541 tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc
    12601 atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact
    12661 catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct
    12721 gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg
    12781 gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg
    12841 aaaacgacaa taatgaattt ttgttcctct tagtatttct accga
//
LOCUS       DAFCHQ010000070        12836 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000070 DAFCHQ010000000
VERSION     DAFCHQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.070"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="LNC71_002160"
     CDS             6..305
                     /locus_tag="LNC71_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6467202.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LNC71_002161"
     CDS             378..1739
                     /locus_tag="LNC71_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBK6467203.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LNC71_002162"
     CDS             1761..2021
                     /locus_tag="LNC71_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467204.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LNC71_002163"
     CDS             2035..2787
                     /locus_tag="LNC71_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBK6467205.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LNC71_002164"
     CDS             complement(2881..3486)
                     /locus_tag="LNC71_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6467206.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LNC71_002165"
     CDS             complement(3539..4513)
                     /locus_tag="LNC71_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBK6467207.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LNC71_002166"
     CDS             complement(4891..5610)
                     /locus_tag="LNC71_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6467208.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LNC71_002167"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LNC71_002167"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBK6467209.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LNC71_002168"
     CDS             7161..8558
                     /locus_tag="LNC71_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6467210.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LNC71_002169"
     CDS             8639..9079
                     /locus_tag="LNC71_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6467211.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LNC71_002170"
     CDS             9227..9553
                     /locus_tag="LNC71_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6467212.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LNC71_002171"
     CDS             9554..10303
                     /locus_tag="LNC71_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467213.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LNC71_002172"
     CDS             complement(10383..10838)
                     /locus_tag="LNC71_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK6467214.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LNC71_002173"
     CDS             complement(11265..11540)
                     /locus_tag="LNC71_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467215.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LNC71_002174"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LNC71_002174"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBK6467216.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4108 a         2212 c         2622 g         3894 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagc
//
LOCUS       DAFCHQ010000071        12809 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000071 DAFCHQ010000000
VERSION     DAFCHQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.071"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..177)
                     /locus_tag="LNC71_002175"
     CDS             complement(<1..177)
                     /locus_tag="LNC71_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6467217.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
     gene            319..933
                     /locus_tag="LNC71_002176"
     CDS             319..933
                     /locus_tag="LNC71_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6467218.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1122..3002
                     /locus_tag="LNC71_002177"
     CDS             1122..3002
                     /locus_tag="LNC71_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBK6467219.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3081..3308
                     /locus_tag="LNC71_002178"
     CDS             3081..3308
                     /locus_tag="LNC71_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBK6467220.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            3313..3855
                     /locus_tag="LNC71_002179"
     CDS             3313..3855
                     /locus_tag="LNC71_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBK6467221.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4082..4798
                     /locus_tag="LNC71_002180"
     CDS             4082..4798
                     /locus_tag="LNC71_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBK6467222.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(4858..6876)
                     /locus_tag="LNC71_002181"
     CDS             complement(4858..6876)
                     /locus_tag="LNC71_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBK6467223.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6889..7887)
                     /locus_tag="LNC71_002182"
     CDS             complement(6889..7887)
                     /locus_tag="LNC71_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467224.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8085..9326
                     /gene="sstT"
                     /locus_tag="LNC71_002183"
     CDS             8085..9326
                     /gene="sstT"
                     /locus_tag="LNC71_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBK6467225.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            9473..10108
                     /locus_tag="LNC71_002184"
     CDS             9473..10108
                     /locus_tag="LNC71_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBK6467226.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            10299..11039
                     /gene="truA"
                     /locus_tag="LNC71_002185"
     CDS             10299..11039
                     /gene="truA"
                     /locus_tag="LNC71_002185"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK6467227.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11184..12782)
                     /locus_tag="LNC71_002186"
     CDS             complement(11184..12782)
                     /locus_tag="LNC71_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467228.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4284 a         2034 c         2508 g         3983 t
ORIGIN      
        1 atcatataga tcatagatat ccttataatg tgtataaaaa gtcgaacgat gtacatctgc
       61 cgtttctgtt agttcgcgta tagtgattgc ttgaatcttt ttatttacta ataatgtgga
      121 tagtgtttca gccaaagctt tttctgtttt tttgacgcgt ctatctgtct ttttcattta
      181 cctatctcct tatccgacat atcaaagcaa agctgtcgct taatcgacaa acttatagat
      241 gattgattat agatagtaga cactgtgtct attataatgt aagtataaca caatcattga
      301 agttgaggag gcgaaatgat gaaactaact tttaaaccct tagaaaagaa agattacaaa
      361 aaagctatct attttgctgt tgaaggaatg aactttgaga gatatatgga aaatgaactt
      421 ctgctgaatc tttacggaag atattttttg tatatggagt tgaatcgagc aactcaaatt
      481 cttgctttgt atgcagagga cgagttggca ggtgttttgc tggcggagat aaaaggagaa
      541 aaaaagaaac atcgttcttt tggacagcaa atatatgtta agatattcga ttttttacaa
      601 aatatatttg ctaaagaaag taaaggagct tacgagacag caaatgaaca aatgcttgca
      661 gcatatttaa agaagcattc gccagatgga gagatcattt ttttagctgc aaatcctgat
      721 ttgaaaacaa aaggcatcgg taccaagttg ctaacagaat ttgaaaaaag agagaaagga
      781 aaagaaatat tcctttttac agatagtggc tgtacgtatc aattctatga acgacgtgga
      841 tttgaaagac taaaggaaaa aattagcaag ataaaaatct tagataaaga aatagatctc
      901 cgatgtatgc tgtacaggaa aatcattcaa tgaaaatttc gatagaaacg acaatagttg
      961 gcaagatcac ttttagtgag tcttcttgct aactattttt tgtagtgtat tctaccaaaa
     1021 gaaaaccgcc tttttgacta aacttgatag tggtcaattt tttatgatgg ataagtatct
     1081 atacttagtc tcgtaagaat gaaaggaaaa gagggagaaa gatgaaatta caaaagtgga
     1141 tccaagagca tcgaaatcat atcaccgcct tatcagctgt gttgattatt tttggtttta
     1201 tcggaaaatt tactggtttt gtcttgttct ataacattgc tctagccata gcttctatta
     1261 ttgcggtcat acctattatg atccatgcgt atcaggcagt gaaagtcaaa gttatctcaa
     1321 ttgatttact cgttactatt gctgtgcttg gtgcctttat gatcggagaa tacaaggagt
     1381 cagctatcgt tacattcttg tttttgtttg gtagctatct ggaacaaaaa acattgacca
     1441 aaacgcggga gtcaatcaag gctttgaccg agatggcccc atctactgct agtgttgtcc
     1501 aagaagatgg aacgactgaa gaagtggaaa tcgatgacgt ggaagaaggc gatatcgtct
     1561 tagtaaaaac tggtggagct attcctgtgg acggacgagt cattgacggg aaagggtatg
     1621 cggatgaagc cgcagtgact ggtgaatcac gacaagtcgt caaaatcagt ggagatgaag
     1681 tcttctctgg aacgatttta gcagatggct acgtgacgat tgaagcaact aaggtcggag
     1741 atgatacgac ctttgccaaa attatcgaat tagtagaaga agcacaagat accaaatccc
     1801 atgcggaaaa attcattgat cgattttctc agtattatac tccaaccgtt ttacttattg
     1861 cgttggttgt tggtctgatt agtcgtgatt ttcgtttagc aatcactgtt ttggttcttg
     1921 gttgtcctgg cgctttagtc attggtgcgc ctgtttcgaa tgtagcagga atcggtaatg
     1981 gagcgaaacg gggcatctta gtcaaaggcg gtgaagcaat ggatactttt tccaaaatcg
     2041 atacttttgt ctttgataaa actggtacat tgaccaaagg aactactgct gtatccacta
     2101 tcaagaacta tacagaggat gaaggggaca gcttagcttt agtggctcga ttagaaacgt
     2161 tatccgatca tcctctgggg cgcgccgtga tccattatgc tcaggagaaa gacattgatt
     2221 ttcaacaatt agcagtcaaa gataatcaga caatcaaggg acaaggaatc acagctgaga
     2281 ttgatggtca tacagtttgt gcgggcaaca ccaaattgat tgccgctcac aaactttctc
     2341 tcacaccaaa gcaagtacaa gatttacatc aattacaaca aacaggcagc tcggtcatta
     2401 ttgtagcaat cgacaaaaaa atcactcaaa tcataggtgt gtcagatgtg attcgaccag
     2461 aagtcgctga acaattagct aagttgaaac aagcaggagc aaaacagtta gtgatgctga
     2521 caggtgataa tcagatgact gctgattatg tagctgatat gttaggcatt gatgaagtcc
     2581 atgctgaatt attaccagac gaaaaggttc aatttgtgaa aaaataccaa gaacaaggat
     2641 tgcgcgttgc ttttattgga gatggaatca atgattctcc ctctttggct gcggctgata
     2701 tcgggattgc tatgggatca ggaacggatg ttgctatcga aacgagtgac gttgtcttga
     2761 tgcaatctaa ttttgaatcg ttggttcatg cctaccgctt ggctaagaaa actgttttaa
     2821 atacccgtga aaatgtagtg attgccattg gtgtcgtctt atttttactc aacggcttat
     2881 ttgccgggtt tatttacatg gctagtggga tgttcgttca cgaagctagt atcttgatcg
     2941 ttatctttaa cgcgatgcgt ttattgtatt ttggacacga aagcaaagaa gaaaaatcgt
     3001 aaaacttgat aagaatcaat ttttttatgg aagctgtaat ctagaataag actatcaaga
     3061 aaaaggaagg aagaactacg atgaaaaaaa ttattatgca attagataca ctagcttgtc
     3121 caagctgtat gcaaaaaatc gaacatgcgg tttctcaaca gcctggggta agcaatacga
     3181 aagttttgtt taatgcgagc aaaatcaaag caaactttga tccagatgaa gtccaccctg
     3241 aacaattagc tgcagttgtg gaaaaattag gttatgaagt ccaatcagca aaagtaaaag
     3301 aggcgtaaat caatgaatat agtggaagaa aaatttgttc aagaaacaaa acaagcagat
     3361 atcgaccatc ataaaccaac tgctggcgcc atggtggggc atgtattagc aaattttaaa
     3421 ttagaagaaa tgaaattgat ccaagcacaa ttgtatgttt tgggtacatc ttcgcaagaa
     3481 ttaagtaccc ttttttcaga atatgctgat caagaagcga gatggttcaa taaaatctct
     3541 catgagttag tgattgaaaa tgaaatcgtt ccgtctactt tagaagaagt catgcgttat
     3601 ggaaaaattt acgaacatgg tcaaaacaaa tatttaccag cctcaacaat gttggaaaac
     3661 tttgcaaaag attttgattt ccaaaatgtc tttatcacac gggcaatcaa attagctgaa
     3721 aaagaagaaa aatatggctt gcaacaattg cttgttgaac ttttaagttt caataaagaa
     3781 atcatctacc aagtacaaag actccaagga aaaactgtcc gacaagattt ggatgaggaa
     3841 gacgatgatg actaaaagtc tatgtttctt caaatacaaa tataaagcgt gaggtaaatt
     3901 ggaaaatcac gaagattaaa ataaaagaag cacccgctga tgtattacag acaacatcag
     3961 cgggtgcttc tcagttttta gaagatttga aagattgatg agaaaatacg aaaaatcaat
     4021 aaaatcacat tgcttatttg atagaataaa gaagtgggag taatcgaaag gagtttttta
     4081 attgaaaaaa aggattttag taacaattgg tctagctttt ttgttagctg gttgtaccaa
     4141 cgggaatgaa tcgaaaaaag aatcaacaaa agaaacagcg aaagcaacag ttgcaactac
     4201 tacaacaact agcacagaga gcaagtcaac tgcagcaagt acagaaacag cagatacctc
     4261 tacttcgtca gagtcttcca aaacgactga aacaactcaa acatctactt caagttcttc
     4321 tgtagaggct tcatcttcct cttcagaaag cagcgaacaa gttgaacaaa cactgtggaa
     4381 tgcagacaaa tccgcacaat tagaggcatt tatgaccaaa tggggccaaa caatgggaca
     4441 acaatacaaa agttatacga atcaaatgag tgtggactta tatggtctta aagtccctca
     4501 agctattttg aacggggagt ggaagatggc tattggtgga gtgcctgttt ctgcagaatg
     4561 gtcagaaagt gggacaggtc aagcagacta tcaaatcaca gccgtctatt ccgatgcaga
     4621 gacagaacca tatctgaaaa agcacgtcta tctattcggg tttcagcaaa atcagccaaa
     4681 agtattggtg acacaacaaa atcaggggaa tcccgataat tatctttact tcaacgagac
     4741 agcgaataat gaattgaaaa atggctttaa tcaaatcgtt tatggacaag caaattaatg
     4801 agataaacaa ggggttgtga catgtctttt atcacaaccc cttgctcagt ttatttgtta
     4861 gtcttttatt ttttgtgtcg cccatgttgc actgtactgg ttacctgcaa caagatcata
     4921 ttgtatcaga cgataatctt ctagaatttc tttttgtttc tcagttaaat ttttttcttg
     4981 gataatttga ctatcctctg taacaaagac ctgttttcca ttaaaacgat tagacaagct
     5041 ttctgtagga tcaattgtta atgcaggtag atgattagat acttctgtca acaacgcata
     5101 gtaaggagtt actttgatat tcgcatgctc aagagccata gcaccaaagt tattaggagc
     5161 aacaacttct ttatttaacg tagctgcttg ttcacgacta tacttgttgt tataaatgaa
     5221 ataatctgtc tggtgctgtt ctaatccata ttttttcgaa tcattcccac tatagattgc
     5281 gggtaaatgg tcaccataaa aaacaaaggt aatcggttta tcgattttgt cgatttcttg
     5341 gatgaatttt tttactgctg tatcggtata gtgaaggcct tgcatataag tttgtaattc
     5401 ttttcttcta ttaggaatga cagcagttcc ttcagcagtg tagttcaatt cactatagta
     5461 atcatcataa ggcatatggt tttgcatagt ggataattgg atgaattgtg acgtttccgt
     5521 attgctttgt aaagctttca aggtttcttt gtatgctgaa tcatccgata tgcgtgggct
     5581 ttgttctatt ttatccgtat aagttaattt atccggactg tctaaataat agaacctgtc
     5641 aaaaccaaat ttatcaaaca cttgttttct gcgataaaga gcagctgtaa aaggatggat
     5701 ggctattttt tcatcgaata gatcggtaat atttggactt attttctgac gctccactag
     5761 ctgggtataa ggtgtaacta acgaagcaga caggttactg atactcaagc ctgtcagtcc
     5821 ttcccattcc atgttagctg ttccgccacc ataaccgacg gataacatca atcctcctgt
     5881 tccttctgtt ataatgcttt tgatgtaagg aatagggtct gtaggtactg tcagattagg
     5941 gacacgtgca ggatcactga agctttcact taaattgaga acgatcgtct gattgtttaa
     6001 ccagtcactt cttgtttggt tgatttcatc tgcctttgtg ttgtactttt tcattattcc
     6061 attaattttt tctttcgaat agccagcagg ttcttccatg attttaatgt cgatattgtt
     6121 taggaattga atgactggtc cgttatcttt tacggcgcct tgctggttaa agaaagtctt
     6181 gtttacttta aataaattaa acagcaagaa agaaggcgaa tttttgtgat taatgaaaaa
     6241 gacacccgaa aaacatccta ataatacagc gatactgata aatcgacgtt tccaatgaat
     6301 cttcaaggaa tactgttttt gtagtctacg ttgtacaata atactagtaa tcaataatag
     6361 gagaaggatc aatccgccca cgattaataa gactgggcta accatcgata atagttctga
     6421 caatccggtc agcattttta aatctgaagg aaagacaggc tcttcccgca tttccatttt
     6481 gattactgtc gaaatagtga gcagtaagtt aatgatcaag gtagcagtca aggcgtacca
     6541 aaaacgattg gaaatcaaca atagtaagaa gaaaaatccc ataataatga tgattgtcag
     6601 gatcatgggt gcttgtcgct gagtaaatat ttttaaggca acctggatag tttctttcca
     6661 accttctttt ggttctaaaa cgaaaatctg cacgaaggta aagcagtaaa aaaatgctgc
     6721 aactaaaaaa taacttttat gaacaaaaag ccaatgaatt ctttttttcc aacgctgagt
     6781 taattgttca atcgaatcaa aagcgaagta ttcgatgaaa cgatgaatga aacggatttt
     6841 ttgtatataa aataaaaaga taactaggag ggacactgct aataaatatc aaataaaaaa
     6901 ctgcccgata tttatcagcc atgccttccc tgagtttgga aattcacgtt tcataaatgt
     6961 agatacgcga taagaaacta aagggaaatt acatataact tgagtgacaa tcaaaaatac
     7021 agcaagaaaa tttcctcttc cttttatacc taatttatca gatagcattt ctaaaaataa
     7081 aaacgaagag aaagtataat acattgcgaa gagagaatat ggtttagaaa acctcagcgc
     7141 tgtatctgta tttcctctga tgatgagtga aatttgtgtc atcaaaataa tcaaaccgaa
     7201 taaaacaatc aagctgataa atacttgagg aagcttatag cggacctttt ctttccagtt
     7261 ccattctttc aaaccataac ccattaaaaa taaataaaaa atccataaaa tactctgtcc
     7321 attctgtaat gcaaacgggt ctttattaaa tagagtagag cagccaacta atccaattgt
     7381 taaaaataat agtagatatt taattgttgt tttcgataat ttatttaaaa atccaactgt
     7441 ataaggaata ctacaaaaaa gcaaagtaca agaaacagca taagtaaagt aattctgact
     7501 gataggtgtt aggataagcc aataatcacg aatattcaag ctgccagctt gtacgtaaaa
     7561 aaagatattg caaactagtg tggctattag gaatataaag gcaaaacgac ttaaataatg
     7621 taaaactttt aaacgggcga ttttttgata ttggtaaccc atgtaaagtg ggatcaaatt
     7681 tgcaccaata acaacggaag tttgtaaaaa tgtatagatg tagaatcgac cagggtttgt
     7741 tgcatatgta ggaatagtga ctgctgaaat caatatacgc tgtaaaacca tcattataca
     7801 agcgacaaag attgtcagga agccataact ctccttcttt atttttttca agaataaaca
     7861 cctcacaaaa aaattggaca aaaacataag aaaacgtttc ctgataagtt gcccattaat
     7921 aacatcctaa cttagaatat ctcaaaaaac aacgtgtaga cagaaaaagt acaaaattag
     7981 caagaaatgg ttgtttgtat tgttagaatt tttagaaaat tataccttta gtttctttgc
     8041 ataaaatgat acaatgattg ttaaaattgt gaaagggtgg tcatttgtta gagaagtttt
     8101 tggatatgtc gttagttcaa cgtattgttg ttggtatttt agtcggaata ttgtttggta
     8161 tctttttccc cacatggaca tttatttcaa tattgggaac gttgtttgtc agttgcttga
     8221 aggcaatcgc acctattctg gtttttttct tgacgatggc gtcaattgcc aagcataaaa
     8281 ttgggaataa gacattcgtg aagcctattt tgatcttata tttagtggga acattgcttt
     8341 ctgcacttgt agcagttatt gtaagttcaa tttttactgt tcctattact ttacaagaaa
     8401 cagtatctga aaaagcaccg caaagtttgg aaagtgtttt atctactatg ttgacgaatg
     8461 tcacacaaaa tcctgttcaa tctcttattg atgcgaatta ccttggcgtc ttattctggg
     8521 ctgtattgtt gggactagct tttcgagcac gttcagaatc gacaaaagag ttggtggatc
     8581 agatatctat agcgttgtca caagtcgtac aagtgattat tgcatttgct ccgataggga
     8641 ttttaggatt ggtttatcaa tcaattgcta cgactggaat cgctggattg gcagagtatc
     8701 tgcagctttt acttgttttg atcggtacga tgctgtttgt cgcattagtt gtgtacccat
     8761 tcctcacgtt tttgtttatt aaagaaaatc cctatccatt gattttcttt tgtttgaaag
     8821 aaagcgcact tcccgctttc tttacccgaa gttctgccgc aaatatcccg ataaacatga
     8881 tgttggctga aagattgaaa ttgacaaaag agtcttattc catatcgatc ccgttaggtg
     8941 ctacgatcaa tatgggcggt gccgcgatta ccatttcctt gatgacgttg actgctgtac
     9001 attctttagg gattgaagca ccttttgttt tgaaagtcct tttatgtatt ttgtctgctt
     9061 tagctgcttg tggagcttca ggtgttgcgg gcggttcact gttattgatt ccattagcat
     9121 gtagtttgtt tggtatctct aatgatgtcg cgatgcaaat cgtcggtatt ggatttatta
     9181 tcggtgttat ccaggattca atggaaactg ccttaaattc gtcaagtgat ctgttgttta
     9241 ctgcaatagg agagctaggc gcgcgtaaaa gaagtgggga aaaaatcaaa ttgaaagatt
     9301 gtttagatgg attaagtgaa cgttgaacgt tagatattta gtatttttaa aatgattcga
     9361 gtttttaatg agttatatag atgttcgtgt aaatagaggt ttctgttaaa tcaagataaa
     9421 gtcgtttata ataacaatta aaagcatagg taattgttat tggaggcgta tcatggattt
     9481 tttacgtaaa tttggttttt tctttttatt attaggcgtg ctgagtgatt ttttgactcc
     9541 gtacgtatta ggattgtatt atcctgaatt agatcaaatg aagagcgtga tcagtgtatt
     9601 tggagaggtt tccagtcctg taagaaaagc gtttcttatt tggtctgtgg tttcagggat
     9661 ctttttcatc ctgtcattgc ctgcattgta tgctgcattt gttagtacat ctcgtgtgtt
     9721 agctacccta gttacagcag ccatcgcgtt ctatggtata ggtgattgta tctttacagg
     9781 tttgttcagt atcaataccc atgaatcttc gtggaatgtc tcgacatggg tccataatat
     9841 cggatcaggt ttaggttaca gtggtttttt attgtttcca gttcttcttt tcttgatcta
     9901 tcgaaaaaat gggcaaatgg acgtaagcca cttatacctt ggactgtctg tgatcagttt
     9961 gctttttgcg gcgatttatg gactggcaag aattccttct ataaattctt ggccattact
    10021 gaataaaata ggtttttgcc aaagaatcag ttttttcttt aattatgtgc ccattgcctg
    10081 gttgagtatc ttgcatctca aacagtaaaa cttgagccgt caaattgaac agtcagcgga
    10141 tacttcatcc ggtggctgtt ttttgttttt ctgaaatata tatttccaca gttttcttgt
    10201 atagcttctg tcttagagaa gcaaggagct atattttgtg ctacaatctt aaaaaggtag
    10261 agagaagacc aaatggaaga cagaggagcc actaaaatat gagaaacatc aagttgacaa
    10321 tcgaatatga cggaaaaaga tattcaggat ggcaaagact tggagatgac gataaaacca
    10381 ttcaaggaaa aatcgaaaaa gtattgcatc agatgacaaa tgaagagata gaaattattg
    10441 gatcaggaag aacagatgct ggcacccatg cgcgaggaca agtagcaaat tttaaaacaa
    10501 acacagaaat tggactggca gaaatgatcg actttatgaa tcgttacctt ccgcgggata
    10561 ttgtcatcaa aagaatcgaa gaaatgcctg agcgcttcca ttcaagatat aatgcggtgg
    10621 gcaaaaaata cagctattat gtttggaaca acgtgattcc ttcagcattt gagcgaaacc
    10681 acagctttta tttccctcaa gagttggata tggacaagct gaatgcagct tgcgaaaaat
    10741 tagtgggtac acatgatttc attggttttt ctgcattgaa aaaatcaaaa aaatccacag
    10801 tgcgtacgat tgatgaatta tctatcgaac gaaagggaga aatgcttcat tttacttttg
    10861 tcggagacgg attcttatat aaaatggtgc ggattatcat ggggacatta ctagaaatcg
    10921 gcacaggtgc catgccgata gaaacaatcg atgaaatatt tgaaagcaaa gtgcggcaac
    10981 atgcaggaga aaccgtccct gcacaaggtc tgtttttgga tgaagtttat tatcgataaa
    11041 aatactttga taatggatga aacgcatgtt tcacagtata aatatcgatg ttaaataatg
    11101 tatgaagcaa attaaagaaa tataaaaaaa gactttttag cctgtttatg tgtacagcta
    11161 aaaagtcttt cttaaataaa aatctagtga gaatgttgta cttctcttct cggttgtttt
    11221 tgtttagaaa aagtaaatcg ataaagtaaa aataaaatca aagtaacgat agataaatat
    11281 cttgaagcaa ttgctagttt tgtgtactga taagtaataa cgactttatt tatacctgga
    11341 ggaatagtta attcagttgt gccaaactta gataattttg tttgtactag tttgccatta
    11401 atagaagcca cttgcccttt ataacgataa ataggtgtcg tcaagacagt atttttatta
    11461 ttcgaattat ctaattcaat tgtaagtttg ttactcataa aatgagttgt tggatttatt
    11521 tcctggttac ctagtaaata ttgatcatta ttaatcatat caggatgata gattgactct
    11581 ttgtttgcat aatcagtatg attgtaattt tcagctatag caacagcgtc ttcaggtgct
    11641 ataagtgtta atgaatcttt gttagcatgg atagaattgc ttactcctga gagatgcaat
    11701 attagtgcaa gaaacgtaaa gaccattatt ttccatgaat tatttttagt agaaaaaata
    11761 taatagatca ctatactcat cgtataggct ataaataaag aagaaaaacc atttaaccgc
    11821 cagacaaatt gtagactctt aactggtgta tggttaaaaa gttgccaagg aaataaattg
    11881 gttgaacaaa aaagaacaaa tacaccaaga aagaaaataa ataaatcatc agcagttaat
    11941 ttttttataa aaataaaagc accaatcaat ccgagaaata agaataatcc aatagtatag
    12001 ttgttatagt tattatttaa aatatgtgtc agcatatcac taggagccat accacttaat
    12061 tcttttccca gtgggacttt caattcttgt gctctagatt gttgaataaa aggaattaag
    12121 aatcccgcac ttaaaaaaaa ggtgaggacg gtagctttta aaagacttaa gagtctagcg
    12181 attttttggt cccaaaaata aaaagaaaga aacaacgttc ctccaataaa tacagaaacc
    12241 atagcgacag aaagtaaatg agtatacact actagcgtca tgccaataga caaccagtac
    12301 catttttggt aatctctaat ataaatttcg taacacccca ttagtatcaa aggcaaaaat
    12361 gttaaagcga cagcttctcc caatgaagca cgatggaata tgtcaatggc acgataggcg
    12421 gaaaatgtat aaataatcga aaataacagc gaaatataac gattgttttt tatttgtttc
    12481 atagaaaaat aagaaacgac cattgttaaa aaagtgataa aaaaatagta gatattataa
    12541 cttaaaacaa gatttcccat cattttataa aataaaaaag ctggataaag agtaagccat
    12601 ggataaaaaa tattcatcat tgttccatga tgatcgaagt tattgaagtt tactggagaa
    12661 ctccatacat tatctagtcc tatgattcga gataaatgaa agtaagtatc ctgattgaca
    12721 ctatgataga ttcccttaga catgaataga gggatgacgt aagcaaaact tagaaataac
    12781 ataaaaatga tatctattat ttggttttt
//
LOCUS       DAFCHQ010000072        12747 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000072 DAFCHQ010000000
VERSION     DAFCHQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.072"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="LNC71_002187"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="LNC71_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBK6467229.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="LNC71_002188"
     CDS             complement(323..892)
                     /locus_tag="LNC71_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBK6467230.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="LNC71_002189"
     CDS             1078..1980
                     /locus_tag="LNC71_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6467231.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="LNC71_002190"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="LNC71_002190"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBK6467232.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="LNC71_002191"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="LNC71_002191"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBK6467233.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="LNC71_002192"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="LNC71_002192"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBK6467234.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="LNC71_002193"
     CDS             8554..8739
                     /locus_tag="LNC71_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBK6467235.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="LNC71_002194"
     CDS             complement(8801..9997)
                     /locus_tag="LNC71_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBK6467236.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="LNC71_002195"
     CDS             complement(10167..11603)
                     /locus_tag="LNC71_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBK6467237.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="LNC71_002196"
     CDS             11896..12705
                     /locus_tag="LNC71_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6467238.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAFCHQ010000073        12600 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000073 DAFCHQ010000000
VERSION     DAFCHQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.073"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..893)
                     /locus_tag="LNC71_002197"
     CDS             complement(324..893)
                     /locus_tag="LNC71_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6467239.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="LNC71_002198"
     CDS             complement(967..2256)
                     /locus_tag="LNC71_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467240.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="LNC71_002199"
     CDS             2610..3458
                     /locus_tag="LNC71_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBK6467241.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="LNC71_002200"
     CDS             3472..4545
                     /locus_tag="LNC71_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBK6467242.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="LNC71_002201"
     CDS             4535..6706
                     /locus_tag="LNC71_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBK6467243.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="LNC71_002202"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="LNC71_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBK6467244.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="LNC71_002203"
     CDS             complement(9117..11303)
                     /locus_tag="LNC71_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBK6467245.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="LNC71_002204"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="LNC71_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBK6467246.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="LNC71_002205"
     CDS             complement(11525..11965)
                     /locus_tag="LNC71_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBK6467247.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="LNC71_002206"
     CDS             complement(12039..12512)
                     /locus_tag="LNC71_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HBK6467248.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
BASE COUNT         3537 a         2652 c         2235 g         4176 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
//
LOCUS       DAFCHQ010000074        12201 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000074 DAFCHQ010000000
VERSION     DAFCHQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.074"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(93..650)
                     /gene="frr"
                     /locus_tag="LNC71_002207"
     CDS             complement(93..650)
                     /gene="frr"
                     /locus_tag="LNC71_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBK6467249.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(653..1375)
                     /locus_tag="LNC71_002208"
     CDS             complement(653..1375)
                     /locus_tag="LNC71_002208"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBK6467250.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1511..2392)
                     /locus_tag="LNC71_002209"
     CDS             complement(1511..2392)
                     /locus_tag="LNC71_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBK6467251.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="LNC71_002210"
     CDS             complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="LNC71_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBK6467252.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3632..4111
                     /locus_tag="LNC71_002211"
     CDS             3632..4111
                     /locus_tag="LNC71_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBK6467253.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4328..5419
                     /gene="phnW"
                     /locus_tag="LNC71_002212"
     CDS             4328..5419
                     /gene="phnW"
                     /locus_tag="LNC71_002212"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBK6467254.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5412..5540
                     /locus_tag="LNC71_002213"
     CDS             5412..5540
                     /locus_tag="LNC71_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK6467255.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5544..6089
                     /locus_tag="LNC71_002214"
     CDS             5544..6089
                     /locus_tag="LNC71_002214"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBK6467256.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6543..8234)
                     /locus_tag="LNC71_002215"
     CDS             complement(6543..8234)
                     /locus_tag="LNC71_002215"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBK6467257.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="LNC71_002216"
     CDS             complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="LNC71_002216"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBK6467258.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9716..10735)
                     /gene="argF"
                     /locus_tag="LNC71_002217"
     CDS             complement(9716..10735)
                     /gene="argF"
                     /locus_tag="LNC71_002217"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBK6467259.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="LNC71_002218"
     CDS             complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="LNC71_002218"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBK6467260.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3342 a         2529 c         2203 g         4127 t
ORIGIN      
        1 ataatttttt ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg
       61 gaatgatccg cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta
      121 tcagcagcga tcttatctac ttctttgatg ctgttatctg tcaatgtttg tacgtctttt
      181 tctaatccgc gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca
      241 tcgatcgcat cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc
      301 acatctttag ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg
      361 acatttccat cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca
      421 tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg
      481 attgatgcca tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc
      541 aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt
      601 gctgcttttt tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc
      661 cccttacagt tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat
      721 ttaaattgaa gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat
      781 ccatgacttg taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg
      841 catttgcatc tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca
      901 ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt
      961 acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg
     1021 ctttacggcg aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg
     1081 tcggtacacc cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta
     1141 acatacccat gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc
     1201 gccagatatt tccgccgccc acaacgattg ccatttcaac gcccagctcg tgaacttctt
     1261 tgatttcttg gatgatttct ttgattactg gtggtttgat tccaaatcct gcgtccccag
     1321 ctaaagcttc tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca
     1381 ttcctccaat attttctatt gccattttac catacttaga tgatttcgac actctaagac
     1441 tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta
     1501 ttcaacagaa ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt
     1561 tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg
     1621 caacgtattt ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga
     1681 tttctgcttt gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt
     1741 tgccttcgtt caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg
     1801 gaatttgtga ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc
     1861 ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca
     1921 tatgcaagta tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga
     1981 agctgatttt ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt
     2041 tatctgtttt gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt
     2101 ctgcaatttc ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg
     2161 agtttacttc aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag
     2221 ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa
     2281 ctgctttttc catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta
     2341 cgccagtcat ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact
     2401 cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc
     2461 cagcctttga aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac
     2521 aacatctacg atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac
     2581 tacttgatct tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa
     2641 tttaacggca cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc
     2701 gcagtttgta tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc
     2761 aatacgttct ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc
     2821 gataccgcct aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt
     2881 tttaggtaga acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct
     2941 gatacgtttt tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac
     3001 gaagtattgt cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt
     3061 acctacgaat aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc
     3121 atctactaat ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa
     3181 gatatatttc ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc
     3241 ttctagtaat tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt
     3301 tttcctcttc ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca
     3361 ataagaatgt ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc
     3421 cgtatcgcaa gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa
     3481 aaagaattca agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa
     3541 ataattttag gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata
     3601 catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat
     3661 taaacaagtc attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg
     3721 gcattatggc gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat
     3781 cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac
     3841 tcctgagact aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt
     3901 gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt
     3961 atttgcatct gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt
     4021 cgatacgatc atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct
     4081 tcaccatcca actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc
     4141 gtgttttttt attttcaata ttttccttat tgaataaacc tttacactct ttacaaaaat
     4201 acatcatttt ttattcaaat ttttcaacac cttgattgaa tcacaataat catcatttat
     4261 attggatgtt aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg
     4321 aaatcatatg tataaattat taacttctgg accattgacg acttctgaaa cagtgaaaaa
     4381 tgctatgctg atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat
     4441 aagaaaaaaa ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat
     4501 gcaaggtagc agctccttcg gggtagaatc ggtattaagt tctgtgatca ggccagaaga
     4561 tactgtactg atttgttcaa acggcgcata tagacaacga atgatcaaca tgtgcgaatg
     4621 tctgaaactg aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt
     4681 gattacacag ctgattgaag agaatccgtc tatctccgca ctagcaatgg tccatagcga
     4741 gactacttcc ggtattttga ataatttgga agagattgct gctgtcacac aagaaagaga
     4801 tcttttgttt atagtggacg cgatgtcaag ctttggtggg ataccgattt gtgtagccga
     4861 actagggatt gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt
     4921 ttcttttgta cttgctaata aagcggaact ggaaaaacga aaaaatgtta gccgaagttt
     4981 gtcacttgat ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt
     5041 cacttcccct acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga
     5101 aggaggaatc gaaactcgat attcacgtta tctcgccaat aatcatgtgc tacgacaaaa
     5161 aatgacagag atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc
     5221 ttttctttac cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca
     5281 aaatggattt gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa
     5341 tattggcgag atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa
     5401 ggagaacgtt aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg
     5461 gctgtatggc tcctgtccac gcttttagaa atgcttttct agaaaaagga acccaactga
     5521 ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc
     5581 tttccctccc ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa
     5641 aagaggtgga ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc
     5701 attctacctt gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca
     5761 atattggttc cacgaatgga tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa
     5821 aaaaagaaag gtattcacca gacttcatgg caacttttga agacgtcgaa gggtatggtc
     5881 gaccgtttcc ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg
     5941 aagtaatcaa agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt
     6001 tgacagtagg tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa
     6061 aattagagat ggggaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc
     6121 cggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga
     6181 caagaatcca gaatgacttt tatacaaaat aagaggttgt gaaaaagctg tcctgtatca
     6241 agtaataaaa acagccaagc aaaaatagct cctcatattt ttcgtttggt taggcttatt
     6301 accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg
     6361 acttttgtca cagtcccctt atttgcaaac aaacagtgtt taagagacaa cctctccact
     6421 aaacggtgtt cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag
     6481 ctattttctc ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt
     6541 tcttacatct tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca
     6601 gtcactgcat aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc
     6661 gtatgagcat agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact
     6721 gacggttcgt atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta
     6781 accacttccc agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat
     6841 cctgcttttt ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt
     6901 tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt
     6961 ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt
     7021 tccaaatcag ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat
     7081 acttcttcta ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg
     7141 atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt caaagcccat ttccttcaag
     7201 acagctttca actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt
     7261 ttgaagtcgt aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt
     7321 gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg
     7381 atctctgcac ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca
     7441 tccatcttgt cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga
     7501 tcatagattt tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata
     7561 gcctctttat cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct
     7621 ggttctgttt cagctacttc atggaattgg acgtataaac gaagcaattc attgattggt
     7681 tctgctttaa cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac
     7741 tgtgttcccc agtcacctaa atggttgatg cggattggct gataaccgat tttttccata
     7801 atgaaaccga tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg
     7861 gcaatattag gagaagacat gtcgattggt actgttcctt gattcccgat gttgctatcc
     7921 ccataatgtt ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta
     7981 ttcatgaaaa agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg
     8041 attttttctg ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat
     8101 gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgc
     8161 tcgacttgtt ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga
     8221 tctttattat tcatctttat tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat
     8281 ctctagcgac ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact
     8341 ccaattgtcc taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa
     8401 acagaattct ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa
     8461 ttcgctagcc aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac
     8521 tttcttgaat tatatcagaa aaaaaaacga aaggctagtc gtttcataaa aaaaaaatcc
     8581 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8641 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8701 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8761 tagctgcttc tactttcgga agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt
     8821 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8881 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8941 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     9001 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9061 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9121 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9181 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9241 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9301 ccttcagtac ttcgcccatg gcgttttgta accagtaacc gatagatcct tctgtcattg
     9361 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9421 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9481 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9541 ttgcatccgt agacaagatg gcattgcccc ctaaagcgac aactactttt cgtttggcca
     9601 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9661 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9721 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9781 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9841 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9901 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9961 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
    10021 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10081 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10141 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10201 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10261 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10321 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10381 gttcctctac cattttttga ctgaatccac ggaattcgat tccgtcaaac atacgtccta
    10441 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10501 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10561 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10621 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10681 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10741 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10801 agtaagactg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10861 tgcagcgtgg accaccacga cctcgtgaca attcacttga attgatttct aacactttga
    10921 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10981 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    11041 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11101 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11161 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11221 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgttcgga attttgattg
    11281 ccaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11341 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11401 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11461 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11521 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11581 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11641 catatagact gctagaacgt acttcgatat cttttgtacg aaccccagcc atgattttat
    11701 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11761 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11821 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11881 atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11941 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccgtttca
    12001 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12061 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12121 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    12181 acgtttttct atttttaagc g
//
LOCUS       DAFCHQ010000075        12120 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000075 DAFCHQ010000000
VERSION     DAFCHQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.075"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(223..1005)
                     /locus_tag="LNC71_002219"
     CDS             complement(223..1005)
                     /locus_tag="LNC71_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467261.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1070..1906)
                     /locus_tag="LNC71_002220"
     CDS             complement(1070..1906)
                     /locus_tag="LNC71_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6467262.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1924..3333)
                     /locus_tag="LNC71_002221"
     CDS             complement(1924..3333)
                     /locus_tag="LNC71_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6467263.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3347..5170)
                     /locus_tag="LNC71_002222"
     CDS             complement(3347..5170)
                     /locus_tag="LNC71_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6467264.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6013..6339)
                     /locus_tag="LNC71_002223"
     CDS             complement(6013..6339)
                     /locus_tag="LNC71_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467265.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6385..6747)
                     /locus_tag="LNC71_002224"
     CDS             complement(6385..6747)
                     /locus_tag="LNC71_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBK6467266.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6784..6998)
                     /locus_tag="LNC71_002225"
                     /pseudo
     CDS             complement(6784..6998)
                     /locus_tag="LNC71_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7126..7461
                     /locus_tag="LNC71_002226"
     CDS             7126..7461
                     /locus_tag="LNC71_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467267.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7537..9534)
                     /locus_tag="LNC71_002227"
     CDS             complement(7537..9534)
                     /locus_tag="LNC71_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467268.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9632..9907)
                     /locus_tag="LNC71_002228"
     CDS             complement(9632..9907)
                     /locus_tag="LNC71_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467269.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10139..10873
                     /locus_tag="LNC71_002229"
     CDS             10139..10873
                     /locus_tag="LNC71_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467270.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10912..12054
                     /locus_tag="LNC71_002230"
     CDS             10912..12054
                     /locus_tag="LNC71_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6467271.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         3931 a         2417 c         1848 g         3924 t
ORIGIN      
        1 aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt
       61 aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga
      121 aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata
      181 acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt
      241 aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt
      301 cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact
      361 gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt
      421 atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc
      481 tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc
      541 tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc
      601 tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt
      661 agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc
      721 aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag
      781 cattttgttg tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg
      841 gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc
      901 tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt
      961 agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt
     1021 agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc
     1081 ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt
     1141 cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca
     1201 ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa
     1261 taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt
     1321 tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca
     1381 tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc
     1441 cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc
     1501 agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa
     1561 aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa
     1621 atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga
     1681 cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag
     1741 catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc
     1801 ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat
     1861 ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc
     1921 tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt
     1981 tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg
     2041 cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa
     2101 aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc
     2161 aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc
     2221 attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg
     2281 tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc
     2341 tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac
     2401 atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat
     2461 aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa
     2521 tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc
     2581 taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat cttctggact
     2641 ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt
     2701 caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg
     2761 aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg
     2821 aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc
     2881 agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc
     2941 gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat
     3001 tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt
     3061 tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa
     3121 gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac
     3181 ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc
     3241 atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt
     3301 ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca
     3361 tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac
     3421 tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat
     3481 cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa
     3541 agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt
     3601 ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa
     3661 caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct
     3721 tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt
     3781 caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc
     3841 caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt
     3901 gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa
     3961 caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa
     4021 tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag
     4081 taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt
     4141 tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc
     4201 ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca
     4261 ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa
     4321 ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca
     4381 acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca
     4441 tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat
     4501 aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta
     4561 ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg
     4621 gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg
     4681 ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca
     4741 aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa
     4801 tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa
     4861 tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa
     4921 ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca
     4981 ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg
     5041 ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg
     5101 ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc
     5161 tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca
     5221 atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata
     5281 acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt
     5341 aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta
     5401 gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta
     5461 aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat
     5521 ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag
     5581 taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt
     5641 tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcgata
     5701 gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc
     5761 taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat
     5821 ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa
     5881 attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa tttataggaa
     5941 actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat
     6001 aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg
     6061 gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc
     6121 tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg
     6181 atttaagttg tactgttctt gtaaaatctg tgttaccaat ggtttcgatg acttctctaa
     6241 tccagatgta cctgtcattt ttgaaaaaat gatacctata atagccaaga caacgataac
     6301 tataatgata aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa
     6361 aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat
     6421 tgctccagct acccctgcta tcatcaaaat cgtacccatt tgttcatctt tatctcgata
     6481 taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgctac
     6541 taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat
     6601 ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgcgt ctgctccagt
     6661 tgtctcatct gtttcttgta gtgctccgca aaactggcaa tacttacttt catctgttat
     6721 ttctttgcca cattttctac aatacatgtc ttctcctcct aaaaacattc aattgtttat
     6781 tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa
     6841 tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga
     6901 acttacacat tgctgtgtaa cattcatttt tttgctaact ccgtttgaga aagtcctttt
     6961 tcttttctgt atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt
     7021 atatacaaca atatgatgta tttcaactca taaaatataa aaaaacttca aataccatta
     7081 caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa
     7141 tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct
     7201 gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct
     7261 aacctcgata gcttaaccgc tctttctaga gccctaggaa cccctgtaag ccattttcta
     7321 gacaatgttg tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact
     7381 gaagatgcag ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc
     7441 aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt
     7501 gagatgaaat aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt
     7561 tctcttcctt actctccgtc catcatctct tgttacactt gtaaattgat aaagaggaac
     7621 taattcaact cctgataata tcaattcttt tattacttca tctatccctg ctccttttgc
     7681 taggtagact aaaaaactac tacttgcttt cacattgtat agtctcttaa tatccttaga
     7741 agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgttttctt ttaaagcatt
     7801 ttccacgctg ttattcccaa cattacgtag acgttcacaa acagtgttag taagttcttt
     7861 ctctgtcatt agatatgttc cttgccacga taagccctgt aaaacttcat gtgcttcatc
     7921 taaaatcaat tgtttttcct tgctaagttc tgatcgggaa ttttgaggaa atactctttt
     7981 agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa
     8041 atgaaatttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc
     8101 attaaaaacg tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt
     8161 ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa
     8221 tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc
     8281 ataagtttta ccatagtaaa aactcatttt tttagctaat tgttcccccg acaaaataca
     8341 agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac
     8401 acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc
     8461 aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa
     8521 gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag
     8581 catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat
     8641 ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag
     8701 acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc
     8761 ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt
     8821 catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc
     8881 atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg
     8941 aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata
     9001 tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt
     9061 tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag
     9121 aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa
     9181 cgcagaatta tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc
     9241 atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag
     9301 aataattact ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct
     9361 tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct
     9421 atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc
     9481 taataatttt tctttatcta cataaattgg aatattaatc attgatgttt ccatttgtgt
     9541 ttcctcctta taaaattctg ttaaagacta aaacatacag ataataaaaa caaactcttt
     9601 attactatat gttttagtct tatttgttta ctcaaccttt ataccgttcc atgaaactat
     9661 caatatcaga ttttttaaat ctaatgaccg tatcaatttt tataacaggt aacccgtatt
     9721 gagaaacata ttttctaatc gttcctactg atacacctag atattcactt gcctgctgat
     9781 agtccatata ctctagagtg actccagaaa taatgtcttc tagcataggc tttagcactg
     9841 taattatctc tttcacttgt tgttcaactt gtatagaaac aatgctatca atgatttgta
     9901 gagacatatt tccccctcct ttcgtgattg cttgaacaaa ttttctaaga attatcttcg
     9961 tattttttgc tcaaaataaa agtattttgt tattaaccga ttaatcaaac aaaaaatgat
    10021 taccagttca tcgacataca cataatacct cgataaaaaa gaggtgtcaa tatctttgta
    10081 aaatttattt acaacacccc tttttcgagg tatactaatt ttaggaggtg tggtcaatat
    10141 gataaaacgt ccaccaataa attatttaga acgaaaaaag atactcggaa caaaaataaa
    10201 agctataaga aagagtaaaa aattaacaca accagcattt ggtcttatga ttaacaacgg
    10261 tcaattaatt gacaaaaaaa ctatctacga atgggagaag ggaacctatt taccaatacc
    10321 agagcgatta tcccgaattg ctgatttggg aaatatgagt atagaggagt tagtttgtgg
    10381 caatgtagaa gaatatattt tgggaattat tctttataga gatagcattg tactagatgg
    10441 tataacattt ccagataaaa acctgttcca gcatcttcgt caacagttcc caccagtaca
    10501 ctctaattta gatacatggc tggatagata ttccaaatta gaaccagaga tgcaagaatt
    10561 tattgctaac aagacttgta ataaagttaa aaatgaaaaa attagtcttt tcaatatctt
    10621 aaaaatagaa gaattattca tcaatgctat tgtagaagaa tttgacaata acattctttt
    10681 cctcactagc agtatagaag aactactaga aagaatggta gatgaatggc tacctattca
    10741 attgaaagac atgtcttatc ccgaagaagc tgtgagagaa attactgata atataaacaa
    10801 gctagaacaa acgatttcat caattgggaa aaaatacact aagaaaaaaa tgaagggagg
    10861 tgataccatc tagtggtaag tactccttgt aaactaagag gagatgattt catggcaaaa
    10921 atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg acacattgcc
    10981 accgatccac gaactggtga aaaaataaat actacgagaa gaggttttag tagcaaacaa
    11041 gaagctcaaa aagattatga cgagtacaga catcaaatat tatacggtgt taaaaagaaa
    11101 gcacctgata tgacatttga agacctgtat aatgaatgga taactcatca gcgtaccagt
    11161 gttaaagctt caacaatcgc aatctcagta agatacgcaa ataatcaaat acttccagca
    11221 tttggtaagc ttaaactatc gaatatctct gtcccatatt gtcaaaaagt ggtagatgaa
    11281 tggcacagta aatatgaaag ttacgattat atgagaaaac agactgcaca aattttacgc
    11341 tacggggttg caatgcaata tattgacaat aatcctatgg aaaaaacact tcttccacga
    11401 aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa taatttactg
    11461 gatgctttta aagattttgg taacatgaaa caatacgcct tcttcagatt attggcatac
    11521 acaggtatga gaaagagtga agtactcgct ttacagtgga aagacataga cacgtttaat
    11581 aaagaactac atgtcaataa aactcttgct gtagatgaat ttggtaaagt tataattcaa
    11641 tctccgaaaa caagagcttc tagacgagtt atctctttag atactgagac tctttctatc
    11701 ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa aacttggata caatactagc
    11761 tctaaagagc aacatgtttt tacaacagtt aaaaacactc tatacattcc aaacactgtt
    11821 aatgattggt taagatatat tttgaaaaaa tataacttgc ctcgtattac acctcatggg
    11881 tttagacata cacatgctag tttattatta gaagcaggag aatctgtcaa ggtagttcaa
    11941 caacgattag ggcatgaaaa tagtaaagtg acattagaca tctatgcaca tattacaaat
    12001 aatgcaccta agaaaacagg tcaagacttt gctgatatga tggctcatca gtaaaagagc
    12061 tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa aaaagctaga acccttatat
//
LOCUS       DAFCHQ010000076        11567 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000076 DAFCHQ010000000
VERSION     DAFCHQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.076"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            370..576
                     /locus_tag="LNC71_002231"
     CDS             370..576
                     /locus_tag="LNC71_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBK6467272.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(778..1779)
                     /locus_tag="LNC71_002232"
     CDS             complement(778..1779)
                     /locus_tag="LNC71_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBK6467273.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1784..3697)
                     /locus_tag="LNC71_002233"
     CDS             complement(1784..3697)
                     /locus_tag="LNC71_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK6467274.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3865..4371)
                     /locus_tag="LNC71_002234"
     CDS             complement(3865..4371)
                     /locus_tag="LNC71_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6467275.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4531..4971
                     /locus_tag="LNC71_002235"
     CDS             4531..4971
                     /locus_tag="LNC71_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBK6467276.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4997..6154)
                     /locus_tag="LNC71_002236"
     CDS             complement(4997..6154)
                     /locus_tag="LNC71_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467277.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6157..6513)
                     /locus_tag="LNC71_002237"
     CDS             complement(6157..6513)
                     /locus_tag="LNC71_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467278.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6811..7785)
                     /gene="sgrA"
                     /locus_tag="LNC71_002238"
     CDS             complement(6811..7785)
                     /gene="sgrA"
                     /locus_tag="LNC71_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBK6467279.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7981..8820)
                     /locus_tag="LNC71_002239"
     CDS             complement(7981..8820)
                     /locus_tag="LNC71_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6467280.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9005..9892
                     /locus_tag="LNC71_002240"
     CDS             9005..9892
                     /locus_tag="LNC71_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBK6467281.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10486..11181)
                     /locus_tag="LNC71_002241"
     CDS             complement(10486..11181)
                     /locus_tag="LNC71_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBK6467282.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11165..11563)
                     /locus_tag="LNC71_002242"
     CDS             complement(11165..11563)
                     /locus_tag="LNC71_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBK6467283.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3445 a         2304 c         1733 g         4085 t
ORIGIN      
        1 tgaattaagt atttccttga taacactggc ttttggcctc attttaggtt atcatttgta
       61 attttatttc acagttgata tgattgttat gtaaaataaa aaagtttagg atacatcgaa
      121 tttttgggga aattcttttg ggggtatcct aaatcttttc tattatattc ccatattttc
      181 tattattggt ctagacgtat gatggtaaac tttgtaccta tctgattgct gaatcattaa
      241 ttgcttatca ttagccatct ttgtttcaag ttcgctagat gatcatttta ttcacatcct
      301 aacaaacttg tcacatttat agattcttaa tctatttatc atacaattat aaaaaaggag
      361 gtgctgctta tgaaagctat ccatatttct ttgctttttt tcgctagtgc attgatttta
      421 tttgctattc atctttgttc ccagccttat atcaataaca acggaatatt agtggaacca
      481 gcattctttt gcttaccact cggctttttg tcccttcttg cctccgcagg cttcggattt
      541 gttgcttttt ttaaacgatc aaaacaacaa atctgactta taaaatttac gtaaaagaga
      601 tcgtgacaat aatcgtgtga tattcccaaa tttcgaataa aaggtgcccc ttgccatctc
      661 taactcctca gtcacaagtt acaatattca taaaccatga aaagcttgtt tattgaaatt
      721 gctccttgtg acttggagct aagtgacaga ttacaacctc ttttgcatca taagacttta
      781 ctccttgaaa aacattacta acttttgata tgtttcttgc gcttgtttat tgctgaaatc
      841 aaactgatat tcatggttca aaacagcatt tttctccgca taatctactt ttgtgacttt
      901 cacccccgct ttctcaaata cagaagttac ttcttccatt tgtttcgtaa acgtatttgt
      961 atttccatcc gttaagaatg tgttttgcgt ccaacgatcc gcatgtctga ccaaggctaa
     1021 acttttgacc ttctctgatt gttcaaagtc cttattatca aagtaagccc atccacattt
     1081 ttcataaaga aagctagaga ttttatcact tacttgagta agtgcagaag gatccaacag
     1141 accagacaaa gaaataaact tcgtaatctg cttgttctcc aaagtcggat caagatgatc
     1201 agctttttta atttcttcat tttgaagtgc taacaaatat tctccactaa tttgtgcacc
     1261 tgccgaattt ccaccaatcg aaatattcga ccaatctgct tgatctttta tctctttttt
     1321 agctgcttga atggcttcgt tcaattggat aactggtgta ggatgttttg cttccggtga
     1381 tcgttcataa tctatattta cgatgatttg ctgcgtgtct gaacatagat attccaaata
     1441 atcacgacaa tcatttttat cccctcctac atatgcgcct ccatgcatcc agaaaagaat
     1501 cggtaatttt ttcttttcca ctttcggtgt gtagatatcc atattattat ttttgtactt
     1561 tgaaggatag gatatttctt tttttatatc gatttgttcg attttttgct ggaaatcatc
     1621 tggtcgggca tatacttgtt ctttttcact tgtgtcaaat tgttttctaa caagcaagct
     1681 aatcggcttt ggtgtgattt gattaatcaa aaatacagtg actaaaataa ctacgatgat
     1741 aactggtact gcaatcacta ttcgcttgaa ccatttcatt catttaccct ctctttcttc
     1801 gccaaatcca tgtgataatc gtgctaacag ataaaaatcc acctgaataa atgagaagcg
     1861 aatgtttctt ttctccagtt ttcggcaaca ttcttttttt tgcatctgtc tttttttgta
     1921 ctttcactgt catctgaatc ggttgcgtat catcacttga ttgatcctct cgaggttcat
     1981 tctttccctt attgtctgca ttctctgtcg gatcttgctt ttttggtgga gctactggga
     2041 tttttttgta atagaaaata atagttggta tcgtctcatt aaaaatccct tcagtcggtc
     2101 cttcaacatt tgctaattcg tatccttcga atgtcttttg ctctgtcata taattttctc
     2161 cataagctcc gtaaagctgg atttcgtcct ggtctttgag tagactttta ttgttttcat
     2221 ccacatactt tacgtttatc tgatcatgga acactacttt caaaaagtcc gttacatgtt
     2281 tcgtgtaggc agcatggtca acttcctgtg cctttccatg ccctgctcct tctactaata
     2341 aaagatcttt ataccctcct gataactcat ataatttttg cgcgttagac acaggtacta
     2401 catcatctgc tgttccatgg ataatcaatt taggcaatga atctgacatt ccaatcgtag
     2461 gtagagcagc ttttgtatcc aaatgcatct cctgatcaaa atagtattgt ttcaaaaggc
     2521 ccataaataa atcttcatgc tcttgatccc caatgatgtc taactgattt tttatggcat
     2581 ccggtagtgc gggggctact gtctggctgt acgtaaaacg tagttgttca tcaattgatg
     2641 caaatccgca attttcaata attcctttga cattgacagg aatatctttc gctaatacag
     2701 acatcatcgt agctgctccc atcgaaccac cataaagtaa aatcttttga gatggccgct
     2761 ctcgaacttc ttgatttatc cagtaaagga cgtcatctga ctcatattgt ccataggtca
     2821 cataatcgcc tccgctatta ccagtcgctc tggaatcaac aaataataaa ttatacccgg
     2881 ccttataaaa atcattgtat tcttctgtaa cgatcccgtt attccaattc cctcgaaatc
     2941 cgccatggat catgactgtt ttatctgaac cttgatcgac ataatatgca tatacatcca
     3001 tcattttgcc atcttgtttg tttagtacag tttgatgaag ttgttttgta tttggataga
     3061 agcctgtgtt ttctgtaggc gttgtcccat catctgtacc tccaagcatc gacatcaaca
     3121 cactgttttt tgcaaaagcg atatcgtaca atagataagt agcaatcaga tgtttgataa
     3181 tctctttatc tacaccataa tcattaacag caagctcagt gactaataac tctattaatt
     3241 ttgacttgat gtcttctgtt ggtccattaa gtatttctgt catctgttta ccgaagtaat
     3301 tcaaatattc tttaccattg aaattttcac ctttaccgat atcatcattt tctgtcccat
     3361 tctctttgtc acttacttta gcagaagagg attcgtcgtt tgacattttt gtagctgttg
     3421 cttcttctgt ttgttggtta tctgtggtag cggtcgtttc ttgatctgtg ctagtaacag
     3481 atgtctcaac cgttgtttca gtatttgttt ctgtttgtga agtactcgat ttcgtttcat
     3541 ttccagcatc agtagtttct gtggtttcta cctcccgaga ttccgattcg gaagaaatgc
     3601 tctctccata tactgtccat tgcgggacgc ttgtacttac agcaaataag atgaccaacg
     3661 ctccctttaa tacttgtttt gttctcaagc tattcactcc tttttatgtt tctaggagaa
     3721 ttatatgtaa acgcaaacat tttttataca aaaaataaat ggtttatccc ataaaaatag
     3781 aacaggatta ttttctcatt ttacgtaata aagcagaatg gataaaccag tatccattct
     3841 gcttttttta aacgaagaaa caacttattg ctccaaaaat gtcagaaatc gtttatccca
     3901 aattttctcg taaaaagcga ttgtttcctt tgctttcata aaaggattat cataagttgt
     3961 cgttgctcct ctacacatca taagctggta tcctaatcca tgagcaaact tcacagtcgt
     4021 atctatacaa tactctgctt gtgccccaca taattcgatc gaatgacagt ccaattcagt
     4081 taataaaaca ctcaaattag tcttataaaa tgaattcgca tgggtttttc tcacaaaata
     4141 actgtgctca ggaatagata attccgacaa tagctcccat ggttttgttt gtggcactaa
     4201 ttgagcatct tcgtgttgaa cgaaaataac tggtttccca atcgtttcgt atgttttgat
     4261 tcttttattc actaatgctg tcactgtttc taattcgtgt atcggcgaag aatcaaaaca
     4321 tacccctttt tgtaaatcga tcactactat tgcgtcggct gtgtttgaca tacttgtgcc
     4381 ttctttcttt tttcttgatt taatagtatt tcttatctag ctagttccat tatacgtgac
     4441 aacattttct tattttacac ctctaaatgg cacttttcga ctatttcttc atgttattat
     4501 ttcataaata cttagctatt ggaggaatgt atgatgatta aattgtatac aggttctggc
     4561 tgctcgtctt caagacgcgc aaaacgttgg cttatcgtcc atgatatacc gtttatcgaa
     4621 cagaacttaa ttcgtcaacc actaaccaaa aatgaattta tccacttact aaaattgaca
     4681 aacaacggcg tgaccgaaat gctctctact cattctatta gttataaaaa tcttgttcac
     4741 agaatagatg aactcagttt gagtgaacta tatattgaga ttcgtacatc cccttcttta
     4801 ttgaaaaaac cgattatcta cgaccaatct gcaaaacttt tgactggatt tagcgaagat
     4861 aaaatcagga tgttcctttc taaaactaaa agaaagcaag agctcaacga actcttgctt
     4921 tctggtcatc ttgtcgattt taaagaaaat aatctttttg caatgtctta attttggcat
     4981 aaacaatctg cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct
     5041 cattcctgat ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc
     5101 cattccagta aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat
     5161 cgctaatgac aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag
     5221 tcatagataa tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa
     5281 tatagaatat gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt
     5341 gaaaaatcaa ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta
     5401 ccccactaaa aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc
     5461 tgtcttctgc tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat
     5521 acaaatggat cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa
     5581 tcttgaagtt tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt
     5641 gtactttatc tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca
     5701 taaaataaag gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt
     5761 caaaaaaagg ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa
     5821 atttgttcat ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg
     5881 aaattcttag taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt
     5941 tcgcaaattt ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat
     6001 tttcgtcgaa tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc
     6061 cttctatcgg attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat
     6121 taagttctct taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt
     6181 ttctaaggat tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc
     6241 ctctaaaagt atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat
     6301 catcttgttt tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc
     6361 tgcttgttga atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc
     6421 cttcaccaca tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg
     6481 cctttcattg atatcaataa attcttcaaa caaagtacat actccttttt atctattatt
     6541 tagacttatt ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag
     6601 agatactacc atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt
     6661 tcctgttatt tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac
     6721 aacttcgcca ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt
     6781 attcaatttt agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa
     6841 atagcttgct cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa
     6901 ggttctacta ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc
     6961 ttgatgatca attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct
     7021 ccctgcattt ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt
     7081 tacttttcct gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct
     7141 tcctgtgcta ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt
     7201 gctactttca ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact
     7261 ttcactgctc gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact
     7321 acttgtgcta tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt
     7381 ctccgtgatc gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt
     7441 gatctcatca atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca
     7501 ttgtacgtct tttaaataaa cttccccaat aactttttct gaacttccat taccatccca
     7561 accatatgtt agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg
     7621 aatagtaatg acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga
     7681 aagattctca tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat
     7741 actcagccca agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca
     7801 tataattacc ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca
     7861 gtcagttgtg aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc
     7921 gtttaatcaa tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt
     7981 ttattgataa acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa
     8041 accaacaatc aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt
     8101 tcccccagta attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg
     8161 cgttccgctg actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc
     8221 accaatagcc gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc
     8281 taaaaagata ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc
     8341 aaataatgca gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg
     8401 aaaaaagcca cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact
     8461 cgttatcagc ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata
     8521 taatggacta gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata
     8581 cccccagtct tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt
     8641 actagcaaga aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc
     8701 tccaatccat tcactatttc caataaacat atccgcaagt gcattattga caagaattcc
     8761 taaaacaaaa ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat
     8821 ctttcatccc tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact
     8881 atgtaaatgt tctccatcat agagagaata gatttttacg aactaagaat atctacagca
     8941 accttacgaa aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga
     9001 aaggatggga aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat
     9061 tttactattc tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac
     9121 acttacagtt caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt
     9181 aaaaaaaatg attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa
     9241 ggaagtaaca aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca
     9301 attaaaagca gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc
     9361 tttccaatac ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg
     9421 gaaagaattc tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc
     9481 agataaagca aaaaaagaag caaatgaagg agaaaatttc tccaagcttg ttcaagctta
     9541 tggaaaaaat aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc
     9601 agagattcct actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga
     9661 tatcattcct gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt
     9721 aaaaaaacag gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc
     9781 taccgaagca aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa
     9841 aaaagacaat gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt
     9901 gacccaattg gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt
     9961 tgaggcaaac cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa
    10021 atcactggat ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac
    10081 atgaatcaat gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta
    10141 tcgcttcaaa aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac
    10201 taaaaatatc ccttctggac ttgttcttct tattctgtta tactagagaa acacgaaaga
    10261 tatttttatg cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt
    10321 cagagttaca aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa
    10381 tagttagtaa tagtcaccca aactttaaat taatgaatat tgaatcgtca gatagcctcc
    10441 tcgagttttc gagaaggcta tcgtttattt tatggagaaa ttgattcaga agtaatcagc
    10501 gacctttttt agttcaccaa tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa
    10561 gcccctcact gctttatcaa tcgagggcat ctctaatact tgatctagta gctctttacc
    10621 taacgttatc gcatgagctc cagatcgaat ggctgtagtg acttgttcac aatttttgaa
    10681 actagctgct aatatttttg ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc
    10741 aatcactttc atagcatcaa tgccttgatt ttgcatcctg ttataataag gtgcaatata
    10801 atctgctcca ttattaatag ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt
    10861 aatcaaataa ccttcttttt tcaacttttt gatcgcttga aggcctgctt gattcaccgg
    10921 caccttgata aacgtatctt ttcccagttc ttctatgata actcgagcat cctctatcat
    10981 ttcttcttcc gttcttccta ttgtctgaat atggatagga atctgtccga ttattttttt
    11041 gatttctttc atatgcgaga agaagtttga cgctccttct tttttcagaa ttgtaggatt
    11101 tgttgttacg cctgttaaat tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc
    11161 tgtatcaagc atgatttcca tatagctttc ccccattctg atcctcttga tcgattttaa
    11221 tgattgtccc ctttcttaaa tgcggcattt ccttatcttc tacatacatt gttcctggaa
    11281 gttccggctc atgagatcca tcaaatttta tcgtgatatg tcctaattca ttcaaattag
    11341 tttggacaac atctcctact gctgtgatta catacatttg tttatcaaaa cagagcttca
    11401 tatcacaagt tatttctttt cttacctgat tcaattcaat cttataacaa taatctgtta
    11461 atgtttccgg tgcttcatct ccaaataaaa tcattatttt ttctttcctg aatacttctg
    11521 cttctatacc aacatctgtt acaaccgttt caaagatcgt catctct
//
LOCUS       DAFCHQ010000077        11556 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000077 DAFCHQ010000000
VERSION     DAFCHQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.077"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(364..1482)
                     /locus_tag="LNC71_002243"
     CDS             complement(364..1482)
                     /locus_tag="LNC71_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467284.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1953..3260
                     /locus_tag="LNC71_002244"
     CDS             1953..3260
                     /locus_tag="LNC71_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBK6467285.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="LNC71_002245"
     CDS             3379..4578
                     /locus_tag="LNC71_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBK6467286.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="LNC71_002246"
     CDS             complement(4605..5873)
                     /locus_tag="LNC71_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBK6467287.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6036..6682)
                     /locus_tag="LNC71_002247"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="LNC71_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="LNC71_002248"
     CDS             complement(7090..7593)
                     /locus_tag="LNC71_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6467288.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="LNC71_002249"
     CDS             complement(7716..8480)
                     /locus_tag="LNC71_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBK6467289.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="LNC71_002250"
     CDS             8588..8863
                     /locus_tag="LNC71_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBK6467290.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="LNC71_002251"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="LNC71_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBK6467291.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10630)
                     /locus_tag="LNC71_002252"
     CDS             complement(10430..10630)
                     /locus_tag="LNC71_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467292.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            11435..>11556
                     /locus_tag="LNC71_002253"
     CDS             11435..>11556
                     /locus_tag="LNC71_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467293.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT         3493 a         2423 c         2037 g         3603 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaataacg
    11341 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    11401 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    11461 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    11521 ccgaatggga cgtgctatac aaactattta taatgt
//
LOCUS       DAFCHQ010000078        11473 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000078 DAFCHQ010000000
VERSION     DAFCHQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.078"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..880
                     /locus_tag="LNC71_002254"
     CDS             <1..880
                     /locus_tag="LNC71_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBK6467294.1"
                     /translation="LILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIK
                     IFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKV
                     RREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVI
                     RENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDV
                     HAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTK
                     LAKAEQYRRSLELSKD"
     gene            952..2073
                     /locus_tag="LNC71_002255"
     CDS             952..2073
                     /locus_tag="LNC71_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6467295.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2155..3927)
                     /locus_tag="LNC71_002256"
     CDS             complement(2155..3927)
                     /locus_tag="LNC71_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467296.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3930..5642)
                     /locus_tag="LNC71_002257"
     CDS             complement(3930..5642)
                     /locus_tag="LNC71_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467297.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5757..6425
                     /locus_tag="LNC71_002258"
     CDS             5757..6425
                     /locus_tag="LNC71_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBK6467298.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6492..7280)
                     /locus_tag="LNC71_002259"
     CDS             complement(6492..7280)
                     /locus_tag="LNC71_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBK6467299.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7363..8835)
                     /locus_tag="LNC71_002260"
     CDS             complement(7363..8835)
                     /locus_tag="LNC71_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6467300.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8965..10158)
                     /locus_tag="LNC71_002261"
     CDS             complement(8965..10158)
                     /locus_tag="LNC71_002261"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBK6467301.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10156..10235)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10424..10495)
                     /locus_tag="LNC71_002262"
     tRNA            complement(10424..10495)
                     /locus_tag="LNC71_002262"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10460..10462),aa:Glu,seq:ttc)
     gene            complement(10507..10580)
                     /locus_tag="LNC71_002263"
     tRNA            complement(10507..10580)
                     /locus_tag="LNC71_002263"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10544..10546),aa:Ile,seq:gat)
     gene            complement(10593..10663)
                     /locus_tag="LNC71_002264"
     tRNA            complement(10593..10663)
                     /locus_tag="LNC71_002264"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10629..10631),aa:Gly,seq:tcc)
     gene            complement(10668..10740)
                     /locus_tag="LNC71_002265"
     tRNA            complement(10668..10740)
                     /locus_tag="LNC71_002265"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10705..10707),aa:Phe,seq:gaa)
     gene            complement(10744..10816)
                     /locus_tag="LNC71_002266"
     tRNA            complement(10744..10816)
                     /locus_tag="LNC71_002266"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Asp,seq:gtc)
     gene            complement(10835..10908)
                     /locus_tag="LNC71_002267"
     tRNA            complement(10835..10908)
                     /locus_tag="LNC71_002267"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10872..10874),aa:Met,seq:cat)
     gene            complement(10921..11010)
                     /locus_tag="LNC71_002268"
     tRNA            complement(10921..11010)
                     /locus_tag="LNC71_002268"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10972..10974),aa:Ser,seq:tga)
     gene            complement(11033..11104)
                     /locus_tag="LNC71_002269"
     tRNA            complement(11033..11104)
                     /locus_tag="LNC71_002269"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11069..11071),aa:Glu,seq:ttc)
     gene            complement(11126..11213)
                     /locus_tag="LNC71_002270"
     tRNA            complement(11126..11213)
                     /locus_tag="LNC71_002270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11177..11179),aa:Ser,seq:gct)
     gene            complement(11238..11311)
                     /locus_tag="LNC71_002271"
     tRNA            complement(11238..11311)
                     /locus_tag="LNC71_002271"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11275..11277),aa:Ile,seq:gat)
     gene            complement(11325..11395)
                     /locus_tag="LNC71_002272"
     tRNA            complement(11325..11395)
                     /locus_tag="LNC71_002272"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11361..11363),aa:Gly,seq:tcc)
     gene            complement(11397..11472)
                     /locus_tag="LNC71_002273"
     tRNA            complement(11397..11472)
                     /locus_tag="LNC71_002273"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11437..11439),aa:Asp,seq:gtc)
BASE COUNT         3764 a         2378 c         1953 g         3378 t
ORIGIN      
        1 tcttatttta aataaacgag agaaaaataa tttaaagaaa aaaatgctca ttccatttga
       61 ttcagaagtg aaaggattac tcaataaact aactatttct aatatagact cgactaggga
      121 aaaaatcata aaaatatttg ataaaattaa taatttattc aagaatgaca atgctgaatt
      181 aaggaagggt cttttagagt ttaaacttca agaactagaa cttcattact cttatttagt
      241 gaaaattacc gaagaaaaag aacaacaaaa agcaataaaa gaacagatga tagaagagga
      301 aaaagttcgt agagaaattg atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt
      361 caattctgaa atttccaaat taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa
      421 agaattatat gcaaataaaa ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat
      481 acgtgaaaat gtattacaaa gagaattaaa tactagagcc ggttatgtgt atgtaattag
      541 taatattggg tcttttggtg aagacatata taaaattggt atgactagaa gattagaacc
      601 tatggataga ataaaagaat tgagtagtgc atcggttcca tttgaatttg acgttcatgc
      661 gatgattttt tcagaggacg ctccgtcttt ggaaactaaa ttgcataatc attttagaaa
      721 acaagaagtt aataaaatta atcaaagaaa agaatttttc aaagtttcac tagacgaaat
      781 agagaaagtt gttttagaaa attataatgg aacagtaaca tttacaaaat tagctaaagc
      841 tgaacaatat agacgatctt tagaattgtc aaaagattaa atatataaaa agccttcggg
      901 ctttttcttt taacaattaa taggaacgta tattcgaaag gaatagaaat aatgtggtta
      961 gaaaaaataa atgacaaaaa atttaagtac accgaacgtt atacggatcc cttaactgaa
     1021 aaaaagagaa aagtgtccgt tactttatct agcaattcac gccaagcttg gaatcaagct
     1081 aacttacttt tgaatgaaaa aatcgcagaa aaaataaaac gaaatgaaga attaccacta
     1141 acttttggag aattaaaaac taaatgggat gaaaaataca agccaactgt aaaagaaagt
     1201 tcctatcgaa caactcaggt ttatctatca ttaatttcaa aatatataaa agatgatgta
     1261 cttgtaaaaa acgttaatag taatcttata caagatatgc tagattacta ctattttgaa
     1321 aaaaatttat catataatta tgtaatacat ttaaaaactt ttacaggaat gatttttaaa
     1381 ttcgccaacc gtcgatatgg attaaaatat aatcccgtaa atggggtttc tttagcaaga
     1441 aaacctaaga caaacgaaga aataaaaaaa gaaaaagatg gttatttatc aaaagaagaa
     1501 gttcaaatga ttgctagtaa acaaaaaagt tcacatcaac agtcacgtta ttctttaatt
     1561 actaaatttt tgtttttaac tggactacgt tatggtgaat tgatatcttt gactgaatct
     1621 gattatgatg gtaataaaat tacggtttct ggtacttatg attacgagtt aaaaattaaa
     1681 acaacaacta aaaatacagg ttcttataga accattgaat tatcaagtaa tgctaaagaa
     1741 atcatagatc aattaattga agaaaacaaa ttaattaaga ataataaaga taaatacatt
     1801 ttcatctcta aaaatggtaa cccgatatct attcaagcat ataatcaatc tttaagagca
     1861 gtatccaaag aattaaacct agataaaaaa gtttcttctc acatgttaag gcattcccac
     1921 atttctctac ttacagaatt aggaatccca ttaaaagcca ttatggatcg tgtaggacat
     1981 gaagattcaa aaacaacatt aaagatttat actcatacaa ctaaaaatat gcaaaatcaa
     2041 ctagtagaaa aactaggaaa aatagaaatt taaattgccc cttttctgcc ccttataaaa
     2101 acaaacaaca taaaatatat aaaaagaacc ccgttataac gaggttcttt taatttaact
     2161 ttcaaataca aattgattat gataaagttc cgcataaaat ccattttctg ctaataactc
     2221 gtgatgactg ccttcttcaa tgacttcccc atcacgcaag acaacgattc tatctgcatt
     2281 caagattgtt ttcagccggt gagcaatcac aaagctggtt cttcctttga ttgcttcatc
     2341 cattgctttt tggatccttg cttctgttac cgtgtctaca ttacttgttg cttcatccaa
     2401 aatcaataaa gatggatttg tgatgatcgt tcgagcaata ctgatcaact gcttttgacc
     2461 tgtactgaat aagttatttt cttcggatat ctctgtatca taaccttttt ctaacgtcat
     2521 gatgaagtca tgaatattcg cttgttttgc tgcattgatg acttcttcat ctgaagcgtc
     2581 cggtttgccg aatgtgatat tgtctttgat cgttccggag aacaatacag attcttgcaa
     2641 aacgattcct acatgtctac gtaatgcatc aagatcataa tctcggatat ctattccatc
     2701 gatgactact gaaccatcat tgacatcgta aaaacggttt agcagattca tgatcgtcgt
     2761 tttccccgat cctgttggac caacaagagc aaccatttct cctttgtcca cattgatcga
     2821 gacatctttc aagactagtt tttctggaga ataaccaaaa tctacgtgct gcaacgcaag
     2881 acctttttca acaccatcaa atgctttaca gttttctggt ttgacttcat ctttttcatc
     2941 aaatatttca ttcaatcgtc ttgcccctgt aatcgctaat tggatcatgc tgtatccaga
     3001 tgaaatctgc atcagtggtt ggtaatattg ttgtgaatac tggacgaatg tcacaactaa
     3061 gcctaatgcc accgaacgtt ccaaatcacc atttaatgct aaccagccgc cgaaaaagat
     3121 aactatcgct gtattgacca gtgacattcc ttgcatcatc ggaaatagta gaccagaata
     3181 agcttgtcct ttgaaggttg cttcgcgaac actttgatta tgctctaaga atccttcaat
     3241 cgtttcttct tgtaagccat ttgtgatgat gacacgttgt ccgctgattt tttcatccat
     3301 ataaccattt agtttcccca cttcatcttg ctgaagatcc acatatttcc gtgctttgcg
     3361 gataatcaag acagctatca aaacggctac tggagtggaa gcaattgtag cccaagctaa
     3421 ctctacattt tggcggaaca tcatgatcaa gatcccgaca agtaaaacag cattcgtgat
     3481 gacttgaagc aaagcttggt tcaaactatt ttgaatatta tctaagtcac tggtaaaacg
     3541 acttaaaatt tcaccgtctt gatgagaatc aaagaaacgg atcgtcaatt tttccaattt
     3601 gttgaataga ccgatacgca ttcggttcgt tgacttccct actacttgtg tgaacaaaat
     3661 actatagata aagttggctg cactagctag tacataaaac agcaagagtt tccatattac
     3721 agaaacaaat ttgcttttat cgtctacgcc ttgcatcaag cctcctacgt aattagctaa
     3781 ttcttgaaaa gcttccccga tgtattgagg tgcctttact tgtagatagg tagcaatgat
     3841 cactgtgata aagataaaga aaaaggagag tttgtaccgc ttcaaatagt gatagaaaaa
     3901 tttacttgct tttattagat cagtcatact tactcctccg ttcctttttg agtttcaaag
     3961 atttcctgat agacacggtt tccagctacc aattcctcat gggttccttc gccgactaat
     4021 ctgccttcat ccaatactaa gatgcggtct gctttgacaa cagaagcaat tttttgtgcg
     4081 atgacaatcg tcgttgttcc cttcaaatct cgatcgagtg cttctcggac cagcttttca
     4141 gagcgagcat ccagtgcact cgtactgtca tctaggatca agatctttgg attaccgata
     4201 acgccacgag taatggataa acgttgcttt tgaccaccag aaaagttact gctacgttct
     4261 tctattggtg cttcgtaacg atcagataac ttttcgatga attcttttgc ttgagcaata
     4321 gaagaagcat gttccatctc tttttctgtt gcattctttt tcccttggcg caagttttga
     4381 gcaatcgttc ctgagaataa aattgctttt tgcaaaacaa aggaaaccgt ttgtcttaaa
     4441 ctcttttcat ttacttgacg aagatctaca ccacctactt caactttgcc ttctgtcgga
     4501 tcaaatagac gagggatcaa ttgtgctagt gttgacttcc cggcaccagt cgccccgacg
     4561 ataccaacca tttctccagg tttgatagaa aagctgatat ctttaagcgt atctgtatcc
     4621 tcaccaggat aacgaaaaga aacatggtca aaagtgacag aacccgtcaa ctcttgttct
     4681 ggaacgtcta aataagtaag atctggttca gcatctaaaa tttccgcaat ccgcttcagc
     4741 gatacggctg ctcttgaagt catcatcatc atcattccgc caataatgat tgccatcata
     4801 atctgcatca agtagttcat gaatgaagca attccaccaa ttaacgtagg atcatcttta
     4861 acaagatcgc tcacaaaaaa gatcgcccca accacagcca aattggcagc cagcatgaag
     4921 gacgggatca tcacagaaaa taatgtaccg actatgatgt tgtgttttgt taagtcctca
     4981 cttactttgc tgaatgactt caattggttt tcttcttgta caaaagattt cactacacga
     5041 atccctagta agttttcttt tgcaatgcta ttcacacgat caatcagttt ttgaatgatc
     5101 atgaagtgct tgcccatcct tgtaaatgaa agtgccgtaa tcagaaatac cgtaacgata
     5161 agcgctacga tgatccacca taattgcggc atcgtgatca tagctaaaat gaaggcacca
     5221 ataaataaga atggaattcg aaaaagtgat tgcaaagaaa tcatcactaa attttggatt
     5281 tgcgtgatat catttgttag acgaacaaca agatttccag cagagaactg ttcgatattt
     5341 ccaaatgaaa atgtctggat ctttcgaaat gcttcttctc ggatatctgc acttacgcct
     5401 tgagctactt ttgcagagaa aatcgtgttc gttacacctg cggctaatcc tagcaaagcc
     5461 agactgatca ggtaaatacc aattgttttc atttcatcat tatcttctgt aataatagct
     5521 tccaatactt gctgtagtaa cttaggttgc cataatgctg ctgcaaccat gactgctacg
     5581 gacagcaagg aaataaaaac agctacttta tattttttcg catgtcgtaa gattaatttc
     5641 attcgttgat tacacccctt tgtttaaatt gacaggacta atttaacact gtaaaattag
     5701 accgactaac aatttaccat gtttaaaaag ataatgcaat acacgaggtt gataaaatgg
     5761 aaataaagct ttctcaagca atcatcattc aggccgcctt tgatatcctg gctgaaacac
     5821 aagctctttc gcagctttcc atgcgaaaca tcgcaaacag aattcatgtt caagcacctg
     5881 ctttatattg gtatttcaaa aacaaacaac agctcttgca aataatggct gaaacaatgg
     5941 aagaagaatt agcgcttcca gatccaactt tgccttggaa tgaacagttg ctgcagtata
     6001 tggaaaatta ctatgacctt tatacacgtt tcccttgtgg ggcagaatta gaaatcaata
     6061 ctgtgcccgc ttttccgagt cgattagaac atttggagca aatgatccaa ttacttgttc
     6121 gtgaaggatt ttcagtcacc caaagtcatc aggccattgc ctctctccac aatttattga
     6181 ttggacagtt aatggatcag cagcatgaac agcagcttcg acaaaaaatt atggacggaa
     6241 atgaattttt gaaagaaaca gttctattta tgcgtaatta tgtagttgaa aatcaattag
     6301 agggatttaa agaaagtatg cagtcccatg cagaattgaa tgaaaaagca ctctttctaa
     6361 cgagtgcaca aacctatatt gaaggcttat gccaacaaaa taaaagaaga gaaaacgagc
     6421 agtaaaaaag ctcctataga gatcactatg tttgtgatct ctataggagc tatcttttta
     6481 agactctttt tttatttttc ttccattagc cacgccaatg cacgttgaat ccattggtcc
     6541 cagaagatcc agttatgatc gccaggtcct tgttcaaagg tgaccgccaa gccttgttcc
     6601 tgcatagcag ataccatttc ctgatttgcc tgataaagaa aatcagattc cccacaagcc
     6661 atataaaatc ttggaactgg ttttccgctt tttactagtt ctttcaataa gtattgcgga
     6721 tcattttttg aaccttcgat ttgatccaat ggtccaaaga tcccttccca aaaagatgct
     6781 ttacgaacca tcaaaagatc atccattcga tttcctacag aaacagcgcc tgaaagagag
     6841 attactgctg cgaaattttc tgattttgca aggcctagtt ttaacgcgcc atatccgccc
     6901 atcgataatc cagcggcata tgttttttct ctttttcttg ttaattgcgg gaataattca
     6961 tgacaaatct caggtagctc ttctgcaatg aatgtccaat acttcatgtc ataaactgta
     7021 tctgtgtacc agccaagatc agtagatggc atgatgacag ctaatccgta gtttgctaca
     7081 taacgttcaa tcgacgtacg tctttcccat acactatgat tgccacccat tccatgaagt
     7141 aaatataaaa caggtatatc cgttgtctct tgctctgaac tggtgccaat cacctttttt
     7201 gttgtttgcg gtagtatcac atttagattg acttccattt ctaacacatt cgaataaata
     7261 tttgcttgaa taaatgccat attttcgcct tccttagtaa gaagttattt ttgaataact
     7321 ttaaatttga atgattcggc tacgtacata tcttaaccga aactactgtt ttattttacc
     7381 acttttttca tgaacgaaaa gcgttaagac caaagcaatg atcacgagta acaagccaac
     7441 tagataaacg gaatgtaacc catcataaag aatttttctt aatggtaaca aaatatccgc
     7501 atcaatatat ttagccgttt gtggattcac cagtttattc atgatatctg catcatttgt
     7561 taaatttgct gctgcaagtt ctcgtttagt taccccattt atcaataaac caaaaataga
     7621 aaccatgact gtttgaccaa ttgttcgaac taacgtatta aaagaagttg ctacaccaag
     7681 ctggtcacga gatacactat tttgtgcact gacagtcgag gtagtcatgg tcacacctaa
     7741 gcctataccg atgaccgttg atatagcaaa aaaccataaa tagttacttc tgaaaggcat
     7801 aagatacaac caagcgcccc caatagctgc gattagtagt ccaattctca agacccaatg
     7861 agttttcatt tgagtcatcc acttgcttgc aaggaaagaa ccgaccatcc agactagtga
     7921 taatggtgct aataccagcc caccaattgc tgcattcagc cctaatacac cttgcatcca
     7981 catagggata taaacatcga ttcccatcaa aaaaccacta gccaatgctg cagccaagtt
     8041 gaccacaaca aatagtggat ttttgaataa atgcatcatg atgacaggat ctttagctct
     8101 tttttcggca aaaacaaaaa tacctaacat tacgatacta cctagcagca aagcagaaac
     8161 aagcggactc attccttcgt ctcctaataa ttggaaccct aacagcaatg agacaagcat
     8221 ggccatcaaa ctaaaactac caaaaatatc cattggttta gactcatgta tgatttttgg
     8281 ttcatttaaa tatagccaaa tcaaaataat taatagaatt ccgatcggca cattgataaa
     8341 aaagatccag tgccagctga tcgtttcaac gatgaatcct cccgcaagcg gtccaaaaac
     8401 actggcaatc ccccaggctg aactattcaa acctaaaact tttgctcgct tttcaataga
     8461 ataaagatcg ccgatgatcg tcaaagctac tggcatcatt gccccagcac ccatcccttg
     8521 aatcgcacga gcagcaatca acccaatcat gctttgagat aaaccgcaaa ataacgaacc
     8581 gataataaac aataatgtcc caaaaataaa gattggtttc cgtcccactt tatccgcaag
     8641 ttttccgtaa atcggagtaa gcatagcgtt tgtcagcaaa taaatcgaaa atacccagtt
     8701 catgatttcc attccatgta atgaaccgac aatcgttggc atagcggttg ttacgattgt
     8761 accttcaata gccgtcatga acgttgcaat gaaaatactc actgtgacca aagtaacatt
     8821 tgtttctttt ttcatcttat tcctcccttt cattatgaca ggatctaaaa atagataaat
     8881 aattactatc tttttcaaga ggttaactgc cataaaaaaa gctggaacaa ccaccacttg
     8941 gcgatcgttc cagctgaata taacttattt tgctaaactt gctttcaacg catctacttt
     9001 gtccgtatgt tcccaaggca aatctacatc tgtacgacca aaatgaccgt aagcagctgt
     9061 ttgtttgtaa atcggacgac gcaaatcaag catctcgatg attcctgctg gccgaagatc
     9121 gaagttttct ctcactgcag caatcagttc ttcttccgga accgttccag taccgaaagt
     9181 attgattgag atcgaaacag gttgagcaac accaatcgca taagctagct gtacttctgc
     9241 tttgcgagca agccctgctg caacaatgtt tttagcaata taacgtgcag catagctggc
     9301 agaacggtca acttttgtcg catctttacc agaaaaagca ccgccaccat gacgagcata
     9361 accgccatac gtatcaacaa tgatttttct tcctgttaat ccggcatccc cttgaggacc
     9421 accaatgaca aatcggccag tcggattgat ataatatttc gtttgatcat ctaataattc
     9481 agctggaata acttccttca ctactttttc gatgacatca tgacgaattg tttcattatc
     9541 tactgcatca tcatgctgtg ttgaaataac gattgtatct acgcgttccg gttgtccttg
     9601 atcatcatat tcaaccgtta cttgagattt tgcatctgga cgtaaatacg ttaattcatt
     9661 tgatttacgc aaatctgcca agcgtcgtac cagacgatga ctcaaagcga ttggtaaagg
     9721 catcaattct ggtgtttcat ctacagcaaa cccaaacatt aaaccttggt caccagcacc
     9781 aatctcatct aatacgtctt ttttatcttc atcccggatc tcaagtgctt catcaactcc
     9841 ttgagcaata tcaggagatt gttcatcaat cgcaaccaat acagctgctg tatctccatc
     9901 aaatccgaat tttgcacgtg tataaccaat ttcttttatt gtttcccgta cgactttttg
     9961 aatatctaca tatgccgtcg tagatatttc tccaaatact aaaacaagac cagttgttac
    10021 agacgtttca cacgctactc gtgctgttgg atcttgtttt aaaattgcat ccaagattgc
    10081 atcactgatt tggtcagcaa ttttatctgg atgtccttca gaaacagatt ctgatgtaaa
    10141 taagtgtctt tctaccatta taatgattcc ccctagttaa attcggttac aaggcatctc
    10201 cgtcctgcgg atcctttcgg gaacttgcaa cgaaccaagt atagcagata aaacagtttt
    10261 ctgctcgtat tttttcattt ttttgcttaa aaaatataaa aatattttac attagaactg
    10321 tccaaaaaag cttagcacaa tcatccataa agataactct gctaagcttt ttaaaatcat
    10381 tctttcagat aaaaagaaaa agatactcaa aaagtatctt ccatgacccg tacgggattc
    10441 gaacccgtgt taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaataa
    10501 aataaatggg cctaaatgga ctcgaaccat cgacctcacg cttatcaggc gtgcgctcta
    10561 accagctgag ctataggccc ataaaaacta aaagcgggtg acgagaatcg aactcgcgac
    10621 aacagcttgg aaggctgtgg ttttaccact aaactacacc cgcataatgg cgcgggacag
    10681 aatcgaactg ccgacacatg gagcttcaat ccattgctct accaactgag ctaccgagcc
    10741 aaacggttcc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg catgttaacc
    10801 cctacaccac ggaaccaatg tttttagttt ttaattgcgg gagcaggatt tgaacctacg
    10861 accttcgggt tatgagcccg acgagctacc agactgctcc atcccgcgat aatatgaaaa
    10921 aggaggataa gggattcgaa cccttgcacg gttttacccg cctgacggtt ttcaagaccg
    10981 ttcccttcag ccggacttgg gtaatcctcc atgtaaaact atattaaatt aatgacccgt
    11041 acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg
    11101 ggccaggtaa aacaaaatat tgaaacggag aaggagggat ttgaaccctc gcgccggttt
    11161 cccgacctac acccttagca ggggcgcctc ttcagccact tgagtacttc cccaaaacca
    11221 ataagatttt gtactaatgg gcctaaatgg actcgaacca tcgacctcac gcttatcagg
    11281 cgtgcgctct aaccagctga gctataggcc cataaaaaac taaaagcggg tgacgagaat
    11341 cgaactcgcg acaacagctt ggaaggctgt ggttttacca ctaaactaca cccgcatggc
    11401 ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta
    11461 caccacggaa cca
//
LOCUS       DAFCHQ010000079        11447 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000079 DAFCHQ010000000
VERSION     DAFCHQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.079"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..54)
                     /locus_tag="LNC71_002274"
     CDS             complement(<1..54)
                     /locus_tag="LNC71_002274"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBK6467302.1"
                     /translation="MLVFQMRNVDKTSTVLKQ"
     gene            complement(103..342)
                     /locus_tag="LNC71_002275"
     CDS             complement(103..342)
                     /locus_tag="LNC71_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBK6467303.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            complement(476..1693)
                     /locus_tag="LNC71_002276"
                     /pseudo
     CDS             complement(476..1693)
                     /locus_tag="LNC71_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            2184..3122
                     /locus_tag="LNC71_002277"
     CDS             2184..3122
                     /locus_tag="LNC71_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6467304.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            3252..3899
                     /locus_tag="LNC71_002278"
     CDS             3252..3899
                     /locus_tag="LNC71_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HBK6467305.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(4592..5530)
                     /locus_tag="LNC71_002279"
                     /pseudo
     CDS             complement(4592..5530)
                     /locus_tag="LNC71_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(5530..6147)
                     /locus_tag="LNC71_002280"
     CDS             complement(5530..6147)
                     /locus_tag="LNC71_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6467306.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(6161..6331)
                     /locus_tag="LNC71_002281"
     CDS             complement(6161..6331)
                     /locus_tag="LNC71_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467307.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(6679..8169)
                     /locus_tag="LNC71_002282"
     CDS             complement(6679..8169)
                     /locus_tag="LNC71_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBK6467308.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(8212..8511)
                     /locus_tag="LNC71_002283"
     CDS             complement(8212..8511)
                     /locus_tag="LNC71_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467309.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8877..9161)
                     /locus_tag="LNC71_002284"
     CDS             complement(8877..9161)
                     /locus_tag="LNC71_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467310.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(9680..10165)
                     /gene="vanZ"
                     /locus_tag="LNC71_002285"
     CDS             complement(9680..10165)
                     /gene="vanZ"
                     /locus_tag="LNC71_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK6467311.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(10318..11229)
                     /locus_tag="LNC71_002286"
     CDS             complement(10318..11229)
                     /locus_tag="LNC71_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBK6467312.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3508 a         2005 c         1743 g         4191 t
ORIGIN      
        1 ctgtttcaaa acagtagatg ttttatctac attacgcatt tggaatacca acatgacgaa
       61 tccctccttc ttaattacaa atttttagca tctaatttaa cttcaattcc tattatacaa
      121 aattttaaga taatgcacta tcaacacact cttaagtttg cttctaagtc ttatttccat
      181 aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat ggtgcaagtc
      241 agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga ttggtgtatc
      301 atttcgtttt tctttttgtg cgcctaaatt tcccacaatc actcacttct ttctatttct
      361 tcttattctt attttatcat caacaatcac aaatcacttg tgatttgtga taagtgattt
      421 gtgataagtg atttgtgatt aatatataaa agccctcttt aaagggcttt tatgtttatt
      481 ttgagaaaga tataaaatca atatatccct tttccccaat ttttacaacg gcattgtagg
      541 gctttttctc tttgtttttg attcctttta ccagggtttc ttttccctcc agtaattctt
      601 ttacattcgt tttggttagt tttttctttc taaaatgttc agctaaagta aacttacatt
      661 caggataatt tgaacaacca taaaacgatt tttttaatac aatattgttg ccacacttag
      721 gacattttcc tacaatactt ttttctgctt ctttttcttt ctgttcctgg taatcagaaa
      781 aatttagttt ttctatatcg ttaggtacag cttccagtaa atgaacaatg aattttttga
      841 tattcgtaat aaagttctct tgattgcctt ctcttttacc gatttttttt aaatacgttt
      901 cccatttagc cgtcatttca gcactcgtta aaaggtgctg actttcaact gcctggcaca
      961 ataattttcc tttttcagtt acaacaagct tattcttaat cacttggata tattcttttt
     1021 gttttaacgc ttcaataata cttgctcttg tcgcttctgt tccaatcccc tcaacttctt
     1081 gtaaaatctt gattgcttct tcatcatcaa ccgttttatt cgccgttttc atagcagtta
     1141 ataatgtacc ctctgtaaaa gcttttggcg gttgcgtttc tttttcaaca ctcttaacac
     1201 caacttcggc acgttcgcca atgataacga gtggtaacgt ttgaacgtcc tctttttctt
     1261 ctttggtttg ttgtttgaaa agaattttcc acccttcttg ctttggtgtc tttccgatcg
     1321 attgaaacag caaatcagct actttcgttt gtatcttggt ttcttcatac aagtaatcag
     1381 gtaaaaacat ggcaacggtc gttttaacga ctaaagcata aatttttcgt tgtaaatcgt
     1441 ccattttcgc taatgcagat tcggtaggta cttgttttgt tgggataatg gcgtggtgtt
     1501 cctgtacctt actaccgtcc acataacgct ttctaggctc tgtttgaacc atttcaagat
     1561 caagtcctaa aaaaccgcta tatttgccaa aattcgcttt taaataagca aattcgttct
     1621 ctgtaataaa tggtgtatct gttcttggat aactcaataa ttttgcttca tacagtcctt
     1681 gcatagcttt taaacgtaaa attttgcgtt ctaagacgtt ttaaatatat ctgcgtatat
     1741 ttttatgtgt tttatctttt cgtcggcaaa tgcgttaaaa tttgcgaaat aaaatagctc
     1801 aagggcgaac gcagtgagtg catttaccct tgatctattt ttttgagtaa attagctttt
     1861 gaaaccgacg gaaaggaaac acaaaacttt tcagaggcaa ggggttttta aggggcgctt
     1921 ttagcccctt gaatttattt gctttaagat atatttagtt ttaaaatagt tttaacgaaa
     1981 aaaagacata aaaaaagacg agcaacgcta ccaacgttct cgcccggaat aacaaaccac
     2041 acttgttatt cattcgatga ttagtatatc aatttttgat tttgaataca aaacaaaatg
     2101 gacgaatgga tacaggcgaa atttattttg cgattccact cgtccttttt tcgttccaaa
     2161 aattatttta ggagcgataa aaaatgaaga aaattgatga aaaattagct gcagaaatct
     2221 cactaaaaaa accggccgtc tctaatagcc ggttaagtaa tagccggtta agtggtcaaa
     2281 ctttgggaaa atctcaaccc gcattaagtt ttgatgccat gacaatcgtt ggaaatttga
     2341 acaaaactaa tgctaaaaag ctatctgact ttatgagtgt agagccacaa atacgacttt
     2401 gggatatact tcaaacaaag tttaaagcta aggcacttca agaaaaagtt tatatcgaat
     2461 atgacaaagt aaaagcagat acttgggata gacgtaatat gcgtgttgaa tttaatccca
     2521 ataaactcac acatgaagaa atgatttggt taaaacaaaa tattatcgac tacatggaag
     2581 atgacggttt tacaagatta gacttagctt ttgattttga agatgatttg agcgattact
     2641 atgcaatgac tgataaagca gttaagaaaa ctgtttttta tggtcgtaat ggcaagccag
     2701 aaacaaaata ttttggtgtc cgtgatagtg atagatttat tagaatttat aataaaaaac
     2761 aagaacgtaa agataacgca gatgttgaag ttgtgtctga acatttatgg cgtgtagaag
     2821 ttgaattaaa aagagatatg gttgattact ggaatgattg ttttaatgat ttacacatct
     2881 tgaaacctgc gtgggctact ttagaaaaaa ttaatgagca agctatggtt tatactttgt
     2941 tgcatgaaga aagtatgtgg ggaaagctaa gtaagaatac taagactaaa tttaaaaaat
     3001 tgattagaga aatatctcca attgatttaa cggaattaat gaaatcgact ttaaaagcga
     3061 acgaaaaaca attgcaaaag cagattgatt tttggcaacg tgaatttagg ttttggaagt
     3121 aaaataagtt ttatttgata aaaattgcta attcagtata attaatattt acgaggtgac
     3181 ataacgtatg aaaaaatcag aggattattc ctcctaaata taaaaattta aaatttagga
     3241 ggaagttata tatgactttt aatattattg aattagaaaa ttgggataga aaagaatatt
     3301 ttgaacacta ttttaatcag caaactactt atagcattac taaagaaatt gatattactt
     3361 tgtttaaaga tatgataaaa aagaaaggat atgaaattta tccctcttta atttatgcaa
     3421 ttatggaagt tgtaaataaa aataaagtgt ttagaacagg aattaatagt gagaataaat
     3481 taggttattg ggataagtta aatcctttgt atacagtttt taataagcaa actgaaaaat
     3541 ttactaacat ttggactgaa tctgataaaa acttcatttc tttttataat aattataaaa
     3601 atgacttgct tgaatataaa gataaagaag aaatgtttcc taaaaaaccg atacctgaaa
     3661 acaccatacc gatttcaatg attccttgga ttgattttag ttcatttaat ttaaatattg
     3721 gtaacaatag cagcttttta ttgcctatta ttacgatagg taaattttat agtgagaata
     3781 ataaaattta tataccagtt gctctgcaac ttcatcattc tgtatgtgat ggttaccatg
     3841 cttcactatt tatgaatgaa tttcaagata taattcatag ggtagatgat tggatttagt
     3901 ttttagattt tgaaagtgaa tttaatttta tacacgtaag tgatcataaa atttatgaac
     3961 gtataacaac cacatttttt ggttgcttgt ggttttgatt ttgaatttgg ttttgaactt
     4021 atggactgat ttattcagtc cattttttgt gcttgcacaa aaactagcct cgcagagcac
     4081 acgcattaat gacttatgaa acgtagtaaa taagtctagt gtgttatact ttacttggaa
     4141 gatgcaccga ataaaaaata ttgaagaaca actagcaaaa gattttaaag agttatttta
     4201 ttttaagtct ttataacatg agtgaagcga atttttaaat ttcgatagaa atttttacat
     4261 caaaaagccc cctgtcaaaa ttgacgaagg gggttttttg gcgcacgctt ttcgttagaa
     4321 atatacaaga ttgaaaatcg tgtataagtg cgccctttgt tttgaactta gcacgttaca
     4381 tcaatttttt aaaatgatgt ataagtgcgc ccttttaaat tttgagtgat tatatttttt
     4441 gagttagaaa aagggattgg gaaaatttcc caaaataatt taaaaaataa gcaaaaattt
     4501 tcgatagaga atgtgctatt ttttgtcaaa ggtgtatacc ttgactgtgc ttgctgttac
     4561 attaagttta tttttaagtt attaaaaaag aaatagcttt taaagtttgg ctcgctgtcg
     4621 ctttataaag ctgattgact tttgattgca aactacttaa agaaaacaaa ctcggactat
     4681 tcgttttctt ctctttggtt tgaacatcag caattatccc ctcttgattg cctattttag
     4741 cttgtttaga agaaacaaaa gctaaaagct cctcttgggt tttaaaacgc tgtgtggggc
     4801 ttagaacgcc cttaaacgac ccttggttta cttttatact agcttccacc tcgaaaaaag
     4861 gttctttttt aaaattctct atggcttcct ggcgctgaaa aataaggtat aaggtgggcg
     4921 tttgaacacg tcctagtgaa aatgtacctt gtacgccctt ctgttgtaaa tttaacgtat
     4981 acaaagggct tgcgttcatg ccgatcaacc aatcggcaat ttggcgtgtt tgcgcttctt
     5041 gataaaaggg atagtaattc attccaggtt gcaaattttg aaaaccgctt cggattacat
     5101 ctttttctaa gctattgatc catagtcttt taaatgtttt atcttttgaa aaggcatttg
     5161 ctttatggat aatcgaccag gcgatatttt caccttctct gtcgctatct gttgcaacaa
     5221 taattgtatt tgcctttttg agaagttctg caacaatttt aaactgcttt cccttatctt
     5281 ttgcaacttc aaaatcgtat cgatcaggaa aaatcggcaa agattcaagt ttccaatttt
     5341 gccacttttc gtcataatga cctggttctg ctaattccac taaatgccca aaaccaaagg
     5401 tgataaacgt ttcatctgta aatagtgggt ctttgatctc aaaataaccg tcttttttgg
     5461 tgctttgttt taaagcactt gcgtaggcta atgcctggct tggtttttca gctaaaataa
     5521 ccgtactcat taactatccc tcttttcatt gttttttctt tgatcgactg tcacgttata
     5581 tcttgctcga taccttctaa acgttcggcg attgattcca gtttgttctt caacttcttt
     5641 atcggataaa ccattcaaaa acaaatcgaa agcatgcttt aaacgtggat cattttcttt
     5701 aaatttatgc tgacgacctt tgaatttgcc ttttttctta gcaatttcga ttccttgtgc
     5761 ctgacgttcc ttaatttttt ttcgttctga ttctgcttga tacttgtaca attcaatgac
     5821 aaggctatta atcaaacgcc ttaaattttc atcttcaata ccattcattg agggtaaatt
     5881 taagacttcc agggttgccc ccttaatttg aatttgattc atcaattctg ttaattcttt
     5941 attatttcgt cctaatcgat ctaattcagt aacaataaca atatcccctt cacgaatata
     6001 gttaagcata gcttgtaatt gtgggcgttc gaccgattga ccgcttaatt tgtctgaaaa
     6061 gaccttagaa acgccctgta acgcttgtaa ttgccgatct aagttctgtt ctttgctact
     6121 gacacgtgca taaccaattt tagccatttt caaccaacct ctaaaattct ctcggttgca
     6181 ataaccaatc agcaatatct actttttcaa tttcaaattg cttatcagaa attgtctttt
     6241 cgtaagcgat aaaatcttgc gcatattgtt gctcattaaa aatagccacc acttcgtcat
     6301 tttctaaaac tcgataaata aattttttca ttttactcct cctattatgc ccaacttaaa
     6361 tgacctattc accaagtcaa ttatactgct aaaatcatat taggacaaat aggtatactc
     6421 taatgaccta taaaagatag aaaattaaaa aatcaagtgt tcgcttcgct ctcactgccc
     6481 ctcgacgttt tagtagcctt tccctcactt cgttcagtcc aagccaacta aaagttttcg
     6541 ggctactctc tccttctccc cctaataatt aattaaaatc ttactctgta tatttctgct
     6601 aatcattcgc taaacagcaa agaaaaaaca aacacgtatc atagatataa atgtaatggc
     6661 atagtgcggg ttttattttc agcctgtatc atagctaaac aaatcgagtt gtgtgtccgt
     6721 tttagggcgt tctgctagct tgtttaaagt ctcttgaatg aatgtatgct ctaagtcaaa
     6781 agaatttgtc agcgccttta tatagctttc tttttcttct ttttttactt taatgatcga
     6841 tagcaacaat gatttaacac tagcaagttg aatgccacca tttcttcctg gtttaatctt
     6901 aaagaaaatt tcctgattcg ccttcagtac cttcagcaat ttatctaatg tccgttcagg
     6961 aatgcctagc acttctctaa tctctttttt ggtcgtcact aaataaggct tgtatacatc
     7021 gcttttttcg ctaatataag ccattaaatc ttctttccat tctgacaaat gaacacgttg
     7081 acgttcgctt ctttttttct tgaatttaaa ccacccttga cggacaaata aatctttact
     7141 ggttaaatca cttgataccc aagctttgca aagaatggta atgtattccc tattagcccc
     7201 ttgatagttt tctgaatagg cacttctaac aattttgatt acttcttttt cttctaaggg
     7261 ttgatctaat cgattattaa actcaaacat attatattcg cacgtttcga ttgaatagcc
     7321 tgaactaaag taggctaaag agagggtaaa catgacgtta ttacgcccta ttaaaccctt
     7381 ttctcctgaa aatttcgttt cgtgcaataa gagattaaac cagggttcat ctacttgttt
     7441 tttgccttct gtaccgctta aaaccgttag acttgaacga gtaaagccct tattatctgt
     7501 ttgtttgaaa gaccaatctt gccattcttt gaaagaataa cggtaattag gatcaaaaaa
     7561 ttctacattg tccgttcttg gtatgcgagc aataccaaaa tgattacacg ttagatcaac
     7621 tggcaaagac tttccaaaat attctcggat attttgcgaa attattttgg ctgctttgac
     7681 agatttaaat tctgattttg aagtcacata gactggcgtt tctaaaacaa aatatgcttg
     7741 ataaccttta tcagatttga taatcatagt aggcataaaa cctaaatcaa tagcggttgt
     7801 taaaatatcg cttgctgaaa tagtttcttt tgccgtgtga atatcaaaat caataaagaa
     7861 ggtattgatt tgtcttaaat tgttttcaga atgtcctttc gtgtatgaac ggttttcgtc
     7921 tgcatacgtt ccataacgat aaacgttggg tgtccaatgt gtaaatgtat cttgattttc
     7981 ttgaatcgct tcctcggaag tcagaacaac accacgaccg ccaatcatgc ttgattttga
     8041 gcgatacgca aaaatagccc ctttgctttt acctggcttg gtagtgattg agcgaatttt
     8101 actattttta aatttgtact ttaacaagcc gtcatgaagc acagtttcta caacaaaagg
     8161 gatattcatt cagctgttct cctttcctat aaatcctata aaataggttg tttaattaac
     8221 ttggtttgct ttttcattca actgtttcaa tattgcatgt tttgaaaaag atttttttcc
     8281 tttataagtc aatttttttc cactaatcga ataaattatt ttgttatttt ctattaactt
     8341 atatatataa tcttccccct ccgaagaaaa atacttatct gattttgttt ctaagtagat
     8401 atttctcttt tctaactctt tcttaaacgt ttctagtgta tagatatttg ctaattttct
     8461 tatctccaat aaactatttt ttatataagt tttacattca tcatgattca tacaaactcc
     8521 accttctata aatgaataca aaaaaagcaa tcaaacgatt tccgattgat tgcttaacaa
     8581 ttcttaaatt cagtagctta gatacttgaa aactctctga tttccctata taatgatagt
     8641 acggttatat accgtcttca aacaaagtta attaaataac ttcttacgag ggaagagttc
     8701 atctgactaa ctgataagcg ttggtttggc aatcttatcg ggctatgcat ttataaaatg
     8761 tcgtcaaaca ttttataaat gtgtcatggc tcttttttcg tttctattca gttcgttgtt
     8821 tcgttatatc tagtataccg cttttaaaaa aaataagcaa cgatttcgtg cattattcac
     8881 acgaagtcat tgcttttttc ttcttccatt tctaaatcca atgttacttg ttctgattct
     8941 gtttctggtt ctggttctgt tggctcattt gggattaaat ccactactag cgttgagtta
     9001 gttaactttg caatttgttc tagtgttttt atggttggat ctgattttcc tgattctatt
     9061 cgtgaataat ttgatctact catttctaat tcttgggaga atttttcttt tgatttggta
     9121 cctctcatct tttttaagct ttctctaaat gctagttcca agtttctccc ccctcttttt
     9181 gtgtaataaa attcacgctt tatttaaatt atagctttta atatcttaat aaacaagtga
     9241 ttaatcactt gtgatttgtg attattccaa aagtaggtaa tttcaccaac ctttttcaaa
     9301 agtttttatc gacgattatt ggactggatc gtgtttccta aagcgattct aagcgcatgt
     9361 ttaattttaa agtgggtaat tctctttaaa acgtcctaca aagattcaaa aaaagcatgg
     9421 ctgctagtgg gggtaccgtc aggaaaatgc ggatttacaa cgttaaggaa atttattaaa
     9481 gagaccaatt cctcagcact aaaattgata attgggcttt ctttgttact atagtgttag
     9541 gtaagctttt tactttatca ccggttttcc cctcacttca cacctacggg cgagtttccc
     9601 cacatcccgt tttccatcta atccaattca ttcaatatct ttatgtgtat gaaagatcag
     9661 attgcttgat ttagacatct tacacgtaat ttattcttaa atgggtacgg taaacgagca
     9721 ataatacgag caaaagtata cctacaaaaa taataactct gtctaatttt ttttgattca
     9781 tatgcttatt gcttaaacca tataatttca gtccaagaaa gcctccaaca gtatttgtaa
     9841 ttacatctgt tatgtctgtc gctccaatag cgaagataaa ttgaattatt tcaaaagtaa
     9901 gacttaaaac cagtacaaaa gcaaacttag gtaaaaatcc gatttcttta aaattgacat
     9961 tcaaaagcaa gccaaatgga ataaagatta taacattatc tatcatctct ctgaaattcc
    10021 cagtagcagt aaatggagtc aagttaagac ttctttgatg ataattaaat actgataaaa
    10081 tattgtattg taatttgaat aacactaacc agattagtgt cactaaatat aaagctagca
    10141 atcctctaga taatattttt cccaatataa tttcctctaa ttcttttgtt taagttgtat
    10201 cattagtata gcaatgtcct agttaacgtg ggttttaaat taaaaagcta acgttacccc
    10261 aaaagtgcta gtacaataat gctgttcatt aatttcgttg gtttcattcc gccatcctta
    10321 cctccttgaa ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt
    10381 gtttcctgat atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac
    10441 aggataataa aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc
    10501 ttttaggtaa tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg
    10561 tggtaaacca acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt
    10621 gtcctctgga taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc
    10681 ttcaggggct cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt
    10741 atgctcacta taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact
    10801 ttgctcatca aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac
    10861 agcatcattg accatctctg aaaatttttg tgctatttct tttgacatat aaatattact
    10921 atcaagcaac ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga
    10981 cttcacactt tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata
    11041 aacctgttct tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa
    11101 atgttctttg ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc
    11161 ttcattaacg tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa
    11221 cttcttcatt ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa
    11281 tcttcccctt aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca
    11341 gtcaattatt tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag
    11401 tgatacccat gctgaattta acgaggctga actgcctgtt gacaatg
//
LOCUS       DAFCHQ010000080        11249 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000080 DAFCHQ010000000
VERSION     DAFCHQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.080"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(33..1076)
                     /locus_tag="LNC71_002287"
     CDS             complement(33..1076)
                     /locus_tag="LNC71_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBK6467313.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="LNC71_002288"
     CDS             complement(1237..1920)
                     /locus_tag="LNC71_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBK6467314.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="LNC71_002289"
     CDS             2115..3044
                     /locus_tag="LNC71_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK6467315.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="LNC71_002290"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="LNC71_002290"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBK6467316.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="LNC71_002291"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="LNC71_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBK6467317.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="LNC71_002292"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="LNC71_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBK6467318.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="LNC71_002293"
     CDS             5429..6232
                     /locus_tag="LNC71_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK6467319.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="LNC71_002294"
     CDS             6316..7863
                     /locus_tag="LNC71_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBK6467320.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="LNC71_002295"
     CDS             complement(8160..8357)
                     /locus_tag="LNC71_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467321.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="LNC71_002296"
     CDS             complement(8433..8633)
                     /locus_tag="LNC71_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBK6467322.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="LNC71_002297"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="LNC71_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="LNC71_002298"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="LNC71_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6467323.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT         3272 a         2201 c         2242 g         3534 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataa
//
LOCUS       DAFCHQ010000081        11226 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000081 DAFCHQ010000000
VERSION     DAFCHQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.081"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            134..307
                     /locus_tag="LNC71_002299"
     CDS             134..307
                     /locus_tag="LNC71_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467324.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            947..1420
                     /locus_tag="LNC71_002300"
     CDS             947..1420
                     /locus_tag="LNC71_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBK6467325.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1429..1656
                     /locus_tag="LNC71_002301"
     CDS             1429..1656
                     /locus_tag="LNC71_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467326.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2292..2663
                     /locus_tag="LNC71_002302"
     CDS             2292..2663
                     /locus_tag="LNC71_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467327.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2919..3161)
                     /locus_tag="LNC71_002303"
     CDS             complement(2919..3161)
                     /locus_tag="LNC71_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6467328.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3193..4095)
                     /locus_tag="LNC71_002304"
     CDS             complement(3193..4095)
                     /locus_tag="LNC71_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6467329.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4108..4296)
                     /locus_tag="LNC71_002305"
     CDS             complement(4108..4296)
                     /locus_tag="LNC71_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK6467330.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4310..4873)
                     /gene="amaP"
                     /locus_tag="LNC71_002306"
     CDS             complement(4310..4873)
                     /gene="amaP"
                     /locus_tag="LNC71_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK6467331.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4911..5804)
                     /locus_tag="LNC71_002307"
     CDS             complement(4911..5804)
                     /locus_tag="LNC71_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6467332.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5882..6805)
                     /locus_tag="LNC71_002308"
     CDS             complement(5882..6805)
                     /locus_tag="LNC71_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBK6467333.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6854..7204)
                     /locus_tag="LNC71_002309"
     CDS             complement(6854..7204)
                     /locus_tag="LNC71_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBK6467334.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7237..8139)
                     /locus_tag="LNC71_002310"
     CDS             complement(7237..8139)
                     /locus_tag="LNC71_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK6467335.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8132..8989)
                     /locus_tag="LNC71_002311"
     CDS             complement(8132..8989)
                     /locus_tag="LNC71_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBK6467336.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9323..10132
                     /locus_tag="LNC71_002312"
     CDS             9323..10132
                     /locus_tag="LNC71_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6467337.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10172..10669
                     /locus_tag="LNC71_002313"
     CDS             10172..10669
                     /locus_tag="LNC71_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBK6467338.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11054..11127)
                     /locus_tag="LNC71_002314"
     tRNA            complement(11054..11127)
                     /locus_tag="LNC71_002314"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11091..11093),aa:Ile,seq:cat)
BASE COUNT         3296 a         2098 c         1879 g         3953 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
      961 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     1021 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     1081 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     1141 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     1201 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     1261 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     1321 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     1381 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     1441 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     1501 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     1561 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     1621 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     1681 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
     1741 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
     1801 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
     1861 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
     1921 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1981 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     2041 gctcccatat atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac
     2101 tatgtagttt tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa
     2161 cattattttg ataataatga ttatttattt aaaaatacat tagataacat ttgttacgac
     2221 acgaaaaagc ttcacaaaca aatatcgtta ttttaaaatg agggcgaact cacacaatta
     2281 ggaggaatag tatgccattt attgtttact tttttctttc attactcgtg atctatattc
     2341 cattaccaac atttttcctg ctcgtcaatc gtctagcaga gcaacatgat acgattgctg
     2401 ctcttacgaa actttgttta cttctatttg tttgtctaga ttactttacg agctattatt
     2461 ggctaaaaca gacaagagta aaaaagagat attatttctt ccttgtcttt ttcaatctct
     2521 ttgagtttct gatccattgc tttttacttc atcagtatca tacgatccat ttgtggatca
     2581 tctgggtttt caaagtggtc gtattatttt gtatgttcac ctttcctatc tcaagaaaat
     2641 ttagggaggc attgttttat taaagaagct tctctgtatc aataaagaca catttaagga
     2701 aaaactttta aaaatctaat tgggaatttg cccgcataaa taagaggctg tgacaaaagt
     2761 cttgtcacag cctcttattc cgtataaaca gtgttcaaaa gcttgcatct tcgataatat
     2821 gcccaaccaa atgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg
     2881 aagaacagct ttttcacgac ctctttttaa aagtcttttt acagttttcg agcaaagaat
     2941 gatacgatta gtactaaaat gatggcacca atcaatgatg gcacgattgc cattcctgca
     3001 atacttggtc cccaagaacc taataatgct tgaccaagtg ctgatccaac taaacctgct
     3061 acgacatttg caataatccc catcgaagaa cctttattcg tgattgctcc tgcaattgct
     3121 ccgataattc ctccgacgat aagtgaccaa ataaatccca tgtttcgttc ctcctaatgt
     3181 tttacttcct tattactgaa cccgtggttc tgtattttct tttacttttt gtgcgccgct
     3241 tccagctgct gattttactt tatctgtttg attcgaagca aattcaccgg tttttttcgc
     3301 tgcgtcagtt actttgtctt gcaccgtttc ttgatctttt tcaaattctt cttttgtttt
     3361 gatgtccact acgttagcgt tgacttcgat cacttctaaa tgtgtcatat tttttacttc
     3421 tttgccgatt acttcttgca tttcttcaaa gattttttcg atatctttgc catattccac
     3481 aatgacatcc atatcaactg ctacttgttt tttaccgact tctgtttcga tcccagccgt
     3541 tacattatct gtattcacaa gttttcctgc cacattagag aagaaaccac cgtttgcaga
     3601 taataaaccg tctacttgtt caattgcgat tccgatgatt ttttgcacaa ctttatcttc
     3661 ataattcagc tcaccgcgca ttgaatcttc ctgttcagaa tcatgctttc cttggttatt
     3721 tgtatgatca gaaccaagtt ttccttgtcc gtttgtgtgg tcagaactaa gtttttcttg
     3781 tgctttcgca tggtcagaag gatgtttttg ctcttccaca ggtttagaag gatcgattgc
     3841 atcatgttgt tctggaactt tagattctgg agttcctgga tgatgaggtg catgcttttc
     3901 tgcatcatgt ttcttctctt ctactccttg ttgaacgtta gggactactg gatctggatg
     3961 tgcagatact ggtgctgttt tgttcttttc atcgttcaca ccatttgatt ggtttggtgt
     4021 cgttgggtct ggatgtgcag acactggttt ttggttgttc attttattgt tattattttt
     4081 gttattgtta ttcatggatg tttcctccta agatttaaaa tagtgatcta agattttcgt
     4141 ttgtttcaga taaaaaccga tagcgacgcc gatcgctgcc atgatcaaga tcaataaggt
     4201 cttgaaaaag cccacggtga tccataaaac ggctaatatc aatccaagta ccccaaagat
     4261 gatagggagc ttgtatgtcg aaaatagttc tttcattttt ggtccccccc tattctactc
     4321 gtttgcggtc attgcttcgt ttagctggtt cttcaaagcc tgtgtatttc accgatactt
     4381 tcactggctc ttttgttccc aatatgtgtt gtacttcatt ttcgatttct ttcatcaatg
     4441 ttcctgtttt accaatgagt gctgaagttc gtttcaactc cccttttaca ttgacggcga
     4501 tcttgttttt cgttgctttg acatttactt ttggtgaatg gatgaattca tcttcatgca
     4561 agtgagaacg aaccattcct tcaattgctt ttttatccaa cgtcaactcc ccatcaccat
     4621 gtttcagtag aaagtttccg attgtttttg gataaaacag gaaaacaagt aacgtgataa
     4681 ttgtcaaaat agcaaatgca acggcaagcc aaaatacata ttgttgcacg tatgaattag
     4741 tgatggaaaa gaatcgaaac ttctctagct gaaaaggcaa tggataggtt gcacttaata
     4801 gtatcgcaaa caaaagcaca gatagcaaaa gtaatgttcc gatcactccg atcgttttta
     4861 cacctttatt cattttcgca cctcctcata ggggtgaaac gatcaacttc ttataagttg
     4921 atcgcttctc caccagttac tccgtaaatt tgggctgtaa cataacttgc tttattcgat
     4981 gctaagaaaa cataaactgg tgctaattct cctggctgac ctgcacgttc tagtaaactt
     5041 tgctgaccaa attcagggat cgaatcagaa ggttgtcctt cgtctagttg taatggtgtc
     5101 cagattggac caggtgctac accatttaca cggattcctt taggtgcaag ctgttttgct
     5161 aaaccgatcg taaagttagc gattgaagct tttgttgctg cgtaatctaa taaatgttcg
     5221 cttggattga atgcttgaac agaagttgtt gttacgattg ctcctcctgc tgggatatgc
     5281 ggaacagctt ctttcactaa agcaaacata gcgatgatgt ttatcataaa tgtgtctttg
     5341 acttgctcaa gtggtaactc agcaatagac tgacaagaaa tttgctgtcc tgcgtttagt
     5401 accatcgtat ctagcccgcc aagttcactc acggctttat ctatgatttc tttaggtgct
     5461 tgttcgtcac gaagatcaaa aggtagtaag acaatcttac gtcctgcaac tttcacgtat
     5521 tttgctactt cctctgcatc tttttcttct cctgggaaga attgaagagc gatatctgcc
     5581 ccttctctag caaatgcaat cgctgctgca cgaccgatcc ctgaatctcc accagtgatt
     5641 agtacacggc ggttttctag ttgattattg ccttcatagg attcttctcc acaatcagga
     5701 acaggaatca tattctgctg taaagctggt gtatcctgat cttgtttagg aaatttctcc
     5761 gtatggtaca agtttcgcgg gtcgattaat tttggttctg tcattgctgt attcctccta
     5821 gtttttaaat tgtattgcca aaacaaattt ctttgtttga tacctgtttt aaatagattc
     5881 tttaatttgt ataatctttc cggtataaat ggtaacctaa aactactgct gctatcaaag
     5941 agccaaagac tacgagtata aagcctagag cagaactttt acccggtaat cctcctgtat
     6001 tcattcccca aatccctgcg aacaacgccg gaacggaaat gattaatgca gctgaatcta
     6061 gatatttcat gatatgattt agattattgt ccatcatccc gttgaataga tcaccaatac
     6121 tctctaataa atctcgatag atctcgatca ttttttctgt ttgtcgctgt ctcaaatgga
     6181 catcatagag tagctgttca tcgtctaaac gtttgatgaa agtttcagaa ttccataatt
     6241 gatccattgt ttcccgttga tctcttaagg tatgatcgat atatacgatg tctctttcaa
     6301 gatctgcctg tttatacagt tcttcatttt ccgttgtttt acgagctgct ttatccaaag
     6361 cgtcaattcg ctttttcatc tctcttagtt ctttcacgta atgggtgtaa atatctaata
     6421 atgtatacgt gatgatcttt tcaaacgagt ttaaatgatt tccatgcttt gtcagcagcc
     6481 gatccacaaa ttcggttttt ctatcgatac aagtaatcaa gagattatcg gaaagtacaa
     6541 aagatattgg cgttaatttt ttttcgatac gattatctga ctgatctaga ttaatcacta
     6601 ccaaagaaaa tggatgctct agtttcgtct cataaaggcg ctccatccga gaaacttctt
     6661 ccggatattc ggttcctacg aaaatatcat caggaagctc gaattcttcg attgcttgct
     6721 caatctcttc gggctttgca ctatcaagta cgagccaatt atattttttt tcatctgttt
     6781 ctattacatt cttttcttca tccacaaagt agtatctcaa tgtatacctc ctttttggac
     6841 tttctcgaaa ctattagctg aagaagcagt tctttaattt gatattataa tcgagtactg
     6901 cgatttcatt ttctaatgaa aagctgtcaa gctgtttgtt gaagtccatg ttcaatcgtt
     6961 taccgaaata attttgctct aatttagatg tatagatgtc caataatgga tgctcgattt
     7021 tagtcgcata ttctccaaat tccgaaacaa aaggaatgat ttgaaaaaag ctgccttttg
     7081 caaaagcacc tttgtccctt agaaatggat cggaatcatt gataagcgcg agtgcgattt
     7141 ttgtattttc cccaaccttt tcaattaacg tctctactgc atttttagac attgtaattt
     7201 ccattcctac tccccctctt cagttgttgt cttctattat ttaacaacta catctgtttt
     7261 tggttgaact tttgcatatt ttggatattt agggaattta gctacacaac ggaaagccgt
     7321 catccctgca tatttttcgt aatatttctt cgttggccct tcaaaatcct tgctccacgg
     7381 tttcacatgt cctgaaaaat ggataatgta aggattgttg cgagtttctt cgtattcacg
     7441 ttcacctgct gctgttggat gtttttttgc ttttgccatg atatagcctt gtgcattcca
     7501 acgcggatga agtggtaacc aacggtcatg taagatagca tttaatgcat cttggtcatg
     7561 gaatcgtaat ttttctggat tatgttcgat gaaatctaat actttttgcg taatattttc
     7621 atctaaccat tttttcacat tgatcagcat caaacctgag ttgaaataac gcattgattt
     7681 agctgaaatt ttcatttttt ccagtcgttg atggaaccca gcgtcttcaa cagctgctac
     7741 gattgaatca ccgaagtcaa gacgccataa tttagagata tcttgtaaag cgatcatgtc
     7801 gcaatccata tataagatac gttcaacttc tgtgccgcgg aacaattccg gaatcgcaat
     7861 acgataataa gctgtttctg gaatacgatc gctgattacc acatttgtaa agaattcttt
     7921 attgattttc aagaattcca catctgagtt cattcgtgcg tttttcactg agaagcgtaa
     7981 aagctgttta ctttcaaaat cgatatcatc gtcaatcaca tagaaccgaa caaatttaga
     8041 cgggttgcaa ttatctaata ctgagacaaa taatgcagct aaatgtggta cgaattttgt
     8101 gttacaactt gcgactactg ctatttcttt tttattcatt gatctcgcct actttcgtta
     8161 gagtactgtg tgctaatttt tttaagtata aattggtata cggatgatct ttcaacgtat
     8221 tccaaggttt atcatgtcct gtaaagtgaa cgatcgaagg tttttcattt ccttctttat
     8281 actgacgttc atatttttca tttggtgctg gatgacgttc aaaaatcagc gaagtctgca
     8341 tgttccattt tggatgaagt tgttcccaat cttcataaag tacagcattc aaagcatctt
     8401 ggtcgtgata gatgattcga tcgccatttt cactgaggta atggatagtt ttttctgtaa
     8461 tttctttttt attccattga tcaatgtcaa tcaccatcac tcctgaattg aagtacaagt
     8521 catcggaatc gattcctaat ctttccaaag ctttcacttg gcctggatcg ataatagcgc
     8581 cgattgtttt tccatttaac ggctcatcat atagttttac gatatcgtct agtactaaca
     8641 catctgaatc taagtaaagg actttcttat aatcctcttt agctaataag tttggtaaag
     8701 aaattcttaa gtaagcggta gttgtgatat ggtcactgac taggaagtct tcgtagatat
     8761 ctttttccac tgtcaggaat tggatacttg cattacttga atatttattt accgtttctt
     8821 ccaatccttc tttactatat tctgatagac catcgtcaat cacataaaat tttatgcgtc
     8881 ttgttttatt gcaattttct aatgcagttg cgatcatcac gctcaagtaa ggagcataat
     8941 tttcgtctga agcagttacc acaggaacta cgccgtatct cttctccatc aaaatcactc
     9001 ctttttctaa tctttttcta acctgttcta catctagaat attcctcatt tggttttaaa
     9061 gcaaaatata tgctttgttt tcattgaata ttttaaaatt tttttatcac aaaacgcatc
     9121 ggtaagcgct aactattcgt ttttcatggc tttttattca aaggtattga ccaaaaacta
     9181 ctatatatag ccaccttttt cttaagaatg ttttttgaaa aaattcataa tgacaaagca
     9241 agttagtttc atttttttaa attctcattt aatcttaagg taacaggaaa atagatatgt
     9301 aaaaagaaag taggaagata aaatgaaact agcagcgatt gatttagacg gtaccttgct
     9361 tgatcgaaat ggaaagatac cagaagaaaa tatccaagca cttcggaaat ttgatgaaaa
     9421 tggcggaatc gtaaccgtcg ctaccggaag gaacagcatt tctgctaaag atattttttc
     9481 cgaattagga atcggtggct atttgatttc ttccaatggt tcactgattg ctgaaatgaa
     9541 ggatggaaaa atggcgaaag ttctcagacg ttcaaaaata gatagcgata cactgagaaa
     9601 agccttcttc tatgctcaag aagcgaaaat atccatcatt gctagccgag aaacccaaga
     9661 tgatcagctg acattcgatg aatcaaatct agtcaaagat gacccttatt atgaacattt
     9721 caatttacag aaccattctt ttgatcaaat tttaaatatg ttggaagatc cttctcttag
     9781 ctatcttaaa ttagcattaa cagataagag tgaagaaaaa ctacagaagc tacaaagaga
     9841 tctgcaagca gatggtatcc aatcagtatt ctctgatccg catttcttag aaatcacgcc
     9901 tacaggtatc actaaagctc attcacttgt ttttctggcg gattaccttg gaatcgaatc
     9961 gagtgacatc atggcttttg gcgatcaaga aaacgacatt gctatgttga attacagcgg
    10021 gttgagtatt gcgatgggga atgcacagcg acaagtaaaa gaaattgcaa accaagtaac
    10081 actgacgaat gaagaagctg gtgttgctta tattttaaaa aacttctatt aaaaagaata
    10141 aaatgacgat atgaatgtag gaggaaagaa aatgttagtt attattgata tgcaaaatca
    10201 tattcttgat ccaaacagcg aattttatat tccagagtca gatcaattgg cagaacgtat
    10261 tgctcaaaga ttagctaaag cacgccaaaa taatgaatat attctcttca cacgggatat
    10321 accagttgaa ctgaaggaca aagaagaaga acgagaagat ttgcaattga tccctcttct
    10381 ttctccacaa gaaaatgaac gagtgattaa aaagtattac tttacgattc cacctgaaac
    10441 tttagctgaa atccaggaaa ctttatttga aagcaaagaa gaacaaaaag agatcgaagt
    10501 agtcgggatt gaaacaaact tgtgtgtact ctctaatctg ataggcctgc agagtgcttt
    10561 tcctgaagca gacttttttg ttgatccaat gttagtctcc agtagaaaac acggagaatc
    10621 cgctttagag ctgttgaaag atttcaacgt ctctatcgtc gaatcataaa aatatctatc
    10681 ttcatttacg atatttaaca cacaataaaa aaagcacgca gaactgtttt agtcctacgt
    10741 gctttttcta tcaaagtaga ttggcttttt tatgacacag agtgaaacgg caggctcacg
    10801 acctctttct taaatggtgt tcaatcagct gaccctcggt attagcttaa caaacggcaa
    10861 aatatgagga actatttttc cgtttgttaa gctaatactc aggtcaaaac gctgatttca
    10921 caacctcttc cttaaacggt attcaaaagc ttgcatctgc agtaataaga acagccaagt
    10981 aaaaacataa gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt
    11041 tcacaacctc ttatgtacct tgtaggactc gaacctacga ccggacggtt atgagccgtc
    11101 tgctctaacc aactgagcta aaggtacatc ataaataaaa tttatttttg ttacttttca
    11161 actatagcac gaatgacaaa ctctcacaag tttcaaaata taagtaaaat aatattgtta
    11221 ttctta
//
LOCUS       DAFCHQ010000082        11197 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000082 DAFCHQ010000000
VERSION     DAFCHQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.082"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..906)
                     /locus_tag="LNC71_002315"
     CDS             complement(13..906)
                     /locus_tag="LNC71_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6467339.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="LNC71_002316"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="LNC71_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBK6467340.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="LNC71_002317"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="LNC71_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBK6467341.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="LNC71_002318"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="LNC71_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBK6467342.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="LNC71_002319"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="LNC71_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBK6467343.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="LNC71_002320"
     CDS             complement(6343..7062)
                     /locus_tag="LNC71_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBK6467344.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="LNC71_002321"
     CDS             complement(7059..8372)
                     /locus_tag="LNC71_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBK6467345.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="LNC71_002322"
     CDS             complement(8453..8773)
                     /locus_tag="LNC71_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6467346.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="LNC71_002323"
     CDS             complement(8881..9168)
                     /locus_tag="LNC71_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467347.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="LNC71_002324"
     CDS             9295..9939
                     /locus_tag="LNC71_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBK6467348.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="LNC71_002325"
     CDS             complement(9998..10705)
                     /locus_tag="LNC71_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6467349.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="LNC71_002326"
     CDS             complement(10925..11095)
                     /locus_tag="LNC71_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467350.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1956 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaac
//
LOCUS       DAFCHQ010000083        11106 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000083 DAFCHQ010000000
VERSION     DAFCHQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.083"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..1327
                     /gene="lysA"
                     /locus_tag="LNC71_002327"
     CDS             68..1327
                     /gene="lysA"
                     /locus_tag="LNC71_002327"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBK6467351.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1314..2114
                     /gene="dapF"
                     /locus_tag="LNC71_002328"
     CDS             1314..2114
                     /gene="dapF"
                     /locus_tag="LNC71_002328"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBK6467352.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2117..2998
                     /locus_tag="LNC71_002329"
     CDS             2117..2998
                     /locus_tag="LNC71_002329"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBK6467353.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2995..3711
                     /locus_tag="LNC71_002330"
     CDS             2995..3711
                     /locus_tag="LNC71_002330"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBK6467354.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3717..4412
                     /gene="dapD"
                     /locus_tag="LNC71_002331"
     CDS             3717..4412
                     /gene="dapD"
                     /locus_tag="LNC71_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBK6467355.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4428..5429
                     /locus_tag="LNC71_002332"
     CDS             4428..5429
                     /locus_tag="LNC71_002332"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBK6467356.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5426..6787
                     /locus_tag="LNC71_002333"
     CDS             5426..6787
                     /locus_tag="LNC71_002333"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBK6467357.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6747..7898
                     /locus_tag="LNC71_002334"
     CDS             6747..7898
                     /locus_tag="LNC71_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBK6467358.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7873..9042
                     /locus_tag="LNC71_002335"
     CDS             7873..9042
                     /locus_tag="LNC71_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6467359.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9217..10596
                     /locus_tag="LNC71_002336"
     CDS             9217..10596
                     /locus_tag="LNC71_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6467360.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10877..11106
                     /locus_tag="LNC71_002337"
                     /pseudo
     CDS             10877..11106
                     /locus_tag="LNC71_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3695 a         1943 c         2493 g         2975 t
ORIGIN      
        1 aaattcaaat ctttatcaaa tgaaattcca ataaaaacgg aaaaaaagtg aaaagaggaa
       61 tgaaaaaatg acagaattga taattggcgg agtcacagca agcacattgg cagaaaagta
      121 taagacacct ctgtatgtgt atgatgaaga aaagatgaga caaacgatgc tgtcattcaa
      181 gcgaggcttt gtatcgaaac gttttgatac aaaagtgctt tacgcctcta aagcattcca
      241 aacgattgag atgctgcatt tagttcgtga atatggattc gggctagatg ttgtcagtgg
      301 tggggaaatc tataccgctt tgcaagctga ttttcctaga gatactatct acttccatgg
      361 taacaacaag acgccagagg aactgatcta tgcacttgaa aatgatttgt tgcatattgt
      421 tgccgataat ttgatggaag ttgaattatt agcagaatta agccaacgct atttaaaaaa
      481 aatctgtatc atgcttcggt tgaatgtcgg agtagaagca catactcatg aatatatcgt
      541 cactgcgcat attgattcaa aattcggcat gagctatgaa agtaaagagt gccaacaagc
      601 ccttcagttg ataaaagaaa gcagctatct agagttagaa ggatttcatg ctcatatcgg
      661 gtcacaaatt tttgatgtta ctgcttggtt agctgaaatc gacaaattga cgaattactt
      721 aaccgatttt gaccaacctc tttccttgaa tcttggcgga gggtttggga ttcgttatac
      781 acaaaatgat cagccgatcc cagtcgaaga ggcactaaaa aaactgattg aatatacgga
      841 agaagctttg gaaaaacgaa aactgaccat tcgtcaatta ctgatcgaac cgggtagaag
      901 tattgttgga gaagctggta caacactata cacgattggt ttcatcaaaa agacaccaca
      961 taaaaaatat tactttgtag atggcgggat gacagataat atccgtccag ctctgtatca
     1021 ggcagaatac gattgtgatt tagcagcaaa actgtcggaa gaaaaaacag agaaagtgac
     1081 gattgcagga aaaatgtgtg aatcaggcga tgtggtaatt aaagaagctt atttgccgaa
     1141 agcagaatcg ggtgatcttt tagctgttta ttccacggga gcttatggat actcgatgag
     1201 cagcaactac aatcgagcga cacgaccagc agttgttttt gtcaataatg gccaatcaag
     1261 actgattgtt cgcagacaag cattttctga tctactaagg ggggaagttt cctatgatcc
     1321 ttcataaata taatggctgc ggaaatgatt ttatcttatt agattacgca gaagacacag
     1381 attatagtca attagccgct tctttatgca caaaagaaaa atatgatata gacggattga
     1441 tcgccgtaaa aatcgatccg ttggaaatga tctattacaa caaagatggc agccgcgcgc
     1501 cgatgtgcgg aaatggcatt cgctgttttg ctcgatacgt aaatgatcag aacatgatga
     1561 atgaacagga attcaacgta gagacattag caggcacaag aaaaattacg gtgttggatc
     1621 cctcaccttt ttattgcagc gtagatatgg gagtgccaga tgattctccg gaaaaaatca
     1681 aagctaatca aacttatcca ataaaaaacc aggaaataga gatcaatggt gaaaaagtaa
     1741 cgatcactag tttattttta ggaacgattc ataccgttgt ttttgttgaa aatgcgcgtg
     1801 aggaagtctg gagaaaaaga ggtgaactga tctgtcatca tcctttgttc aaagaaaaaa
     1861 ccaatgtcaa ctttgtagaa gtagtcaatg aacaagaatt gatcgtccgt acctatgaaa
     1921 gaggagtagg ttggacactt gcttgcggta caggttgctg tgcctcttat gtggttgcta
     1981 aggaaactgg gaaaatttca gcagatcagg tagttgttca cttggaacaa ggagacttga
     2041 tgatctctgg tgaggatgcg atacaaatgg ctggcccagc agttcatgaa tggacaagaa
     2101 tattggagga atgaagatga taaaaggttc gatcgtggcg ctgatcacgc cgatgaatga
     2161 agagggatca gtagattatg ctggtttgga aaaattgatc caattccatt tagatgaaca
     2221 aacagatgga ttattagttt taggtacaac aggagaatct tctacgctca cacaatctga
     2281 ggaggagcag atccttcaac tgacagtaaa gaaagtggct ggccgtgtgc cggtgattgc
     2341 aggagctgga acgaataata cgaaagaaac gattgaaaaa gcgaagcatt ttgcttcttt
     2401 gggggcagac gcacttttgg tcatcacccc ttactacaac aaaacaagtg atgcaggact
     2461 agctgctcat tttacagcaa ttgcagaagc ctcaccgatt ccgcttattt tgtataatgt
     2521 tccttctaga acaggcatgt cgattccaat ccatgtatta gtaaatttag cagaacatcc
     2581 taatatcatt ggtttgaaag aagcctcagg agatatggct tatgtgatgg atgccgctcg
     2641 gttgattgga gaagaattct tcctttacag tggaaatgat gatttgatcc taccagtaat
     2701 gagtgtagga ggaagcggtg taataagtgt atgggcgaat atccaaccta aaatagtcca
     2761 tgaattagta aaagatacgc aggacggaag atggcagcaa gcaaaagaaa agcaattaaa
     2821 tgcacttgaa ctgatccatg ccttgttcag tgagaccaat cctattcctg tcaaagcagc
     2881 catgtcatta ttagacttac cttccggacc tctacgcctg cctttagttt ctctttcaaa
     2941 agagaaaaag aaacaattag ctcagctttt gttaaaagaa aggacggttg aaaaatgaaa
     3001 atcggactga ttggcagtgg acagatggga aatagagtcg aagaagttgc acaagaaaga
     3061 ggagatacgg tccttctttt acggacggca cctaaagaag caactacgct tgcttttacg
     3121 gaactgccag aactgcttat tgatttttct catccagata atttagagaa aatcctttcg
     3181 tatagcttat catatcaact tccgttagtc attggcacga caggttatac acaatcacag
     3241 ttccaagaaa taaaagaaca tgccaaacat gtacctgtga tgtatagtgc taatttttcg
     3301 ttcggcatca tggtgatgaa tcaactaatc aagcaagcag ctgcaatgtt gcaaggctgg
     3361 cagatcgaac tgattgaaaa acatcatagt cgtaaaaaag atgcaccatc tggtactgca
     3421 aatatgctgc ttcaaacaat ccaagaagtg caaaagctgc agcccgtata caactggcaa
     3481 gggaaaaaaa gagaagaaaa ccaaattggt gtacacagta ttcgtgcagg ttctttacct
     3541 ggtgaacatg atgtattgtt tgctgctact gatgaaatat ttacgatcaa acatgaatct
     3601 ttttctaatc gaatctttgc tgcaggcgca gtagaagcag cggactggtt gaaagatcag
     3661 tcaccaggtt attacaaatt agaagattta ttaatggaca aaggggtgta aaaaacgtgg
     3721 aagctcaaga aatcatccgc tacattcaaa cggcatctaa aaagacgcct gtgaaagtct
     3781 atctgaatgt aaccgagcca atcgaattta aagaaagtaa agtttttggt gaaggaagca
     3841 gcttcgttgt ttttggtgac tatgagacga ttgctccggt tttagaagct gaatcagaaa
     3901 aaatcattga aatcgaaatc gaaactgctg caagatacag cgcagttcct ttgctagata
     3961 ttaaaaaaat taatgctcgt atcgaaccag gtgctattat cagagatcaa gtgtcaatcg
     4021 gcaacaatgc cgtgatcatg atgggagcga ttatcaatat tggtgcagta atcggtgaaa
     4081 acacgatgat tgatatgggt gctgtcttag gtggacgagc gacagtgggc aaaaactgcc
     4141 atattggtgc aggagctgtc ttagcaggtg tgatcgaacc agcatcggcc aaaccggtta
     4201 tcgtagaaga cggcgtactg gtaggtgcga atgcagtgat tgttgaaggt gtccatatcg
     4261 gaaaagatgc agtcgtggca gcaggagcag tggttttaga agatgtggca gcagaaactg
     4321 ttgtcggtgg aatacctgca cgtgtattaa aaataagtga tgatcagaca aaagaaaata
     4381 cagcattgat tgctgcgtta cgcgaattat agaggagatg gagaaaaatg cgcgaaatag
     4441 atacctgtat tgtgggggca accggattag tcggccaaac gatgctcaaa gtgttagaag
     4501 aatatgattt tccagtagga cgattgaaat tattagcttc aaaacgttca gccggtaaag
     4561 tttgtgtatt caagaaaaag aaacacactg tacaggaatt gacagaaact tcatttgaag
     4621 gatatgattt agcgctattt tctgcaggtg cttctgtatc caaagagttt gcgccaatcg
     4681 cacgaagtca gggactattt gtcatagata attcctcagc atggcgggaa gattcggata
     4741 ttccattaat cgttccagag gtcaatctgg aagactatac attgaatcgg ctgattgcaa
     4801 atccaaattg ttcaaccatt caagcggtac ttccgcttaa agctttacaa gaagcctttg
     4861 gtatccagcg agtcaattat tcgacctatc aagcagtttc tggttccggg caaaaaggca
     4921 ttgaagatct tcatcgtacg caaaatgacg aggaaccagt tttgtatcct tataatatta
     4981 gccaaacggt gattccggaa atcgatcagc ctttggaaaa tgggtataca aaagaagaac
     5041 agaaaatgat aaatgagacc cgtaagattc tgcatcagcc tgctttgcca gtatcagcaa
     5101 cctgcgtacg tgtgccaatc gaaaatggac atggagtctc tgtggcagtt caactgcaaa
     5161 aagagtttac gacggaagaa attagagagt gtctcagcca gtttccaggg attcaattag
     5221 aagatgatct agcagcacat aaatatccga cgagcattct tgctagagga acagatatgg
     5281 tttatgttgg gaggatcaga aaagacactt ctttagagaa cggattgttg ttctatacga
     5341 ctgccgataa catacgcaaa ggagccgctg cgaatgccgt acagattgct gcagctttaa
     5401 tgcagaaaat agcggaggtg gcaagatgag taatacgctg aaagtagcga aattcggcgg
     5461 aacatcgatg tctcatgaag agcaattcaa aaaagtccgt acaattgttc tttcagatcc
     5521 caatcgccaa gtgatcgttg tttctgcttt aggcaaacgt acgtcagatg atgacaaagt
     5581 aacagactta ctttatttga tttatgctta ccttcagcat catgttgatt ggacacccct
     5641 ttggaaaagg atttgtgatc gtttcattca agtaagagat gttcttgccc tgcagttacc
     5701 tattgaagat gaactagcac gcatacagaa acaactggaa gacgaaaaga taactgaaga
     5761 ttatttagta agtagaggtg aatatctgac tgctcaactg atggctgagt atcttggtta
     5821 ccagtttatt gacgcaaaag acctgatttt ctttgactat gatgggaaaa tcgatcagga
     5881 aaaaacagca caggaaatca caaaagcatt taaaagatat ccgaaaatgg ttgttccagg
     5941 gttttacggg gcgaaccctg caggaaaaga aaaattattg ggccgtgggg gaagcgacat
     6001 taccggcgcg atactggcga agatgttgca agcggaaagt tatgagaatt ggacggatgt
     6061 ttcaggaatc atgatggctg atccgcgaat catcgatcat cctaaaatca tcgaggaaat
     6121 ctcttttaga gaactgagag agatggctta tatgggggct aacgttcttc atgaagcagc
     6181 tgttttccca gttcaagagg ctgatatccc catccaaata aaaaatacca atgatccttt
     6241 cgctcaaggg acaaaaatat cgaatcacga aatagaaaag gaaaatggac tgacaggaat
     6301 agctggacgc aaggattttc tttccatcac tttattcaaa cgtcatatgt ccgatgaaat
     6361 cgggtttatc tggaaagcaa tgagcatttt tgctaaacat gagatatcaa tcgaacatat
     6421 tccttcaggg atcgacaata tcggagtgat cgtctcagca gaagcgattg ccgataagtt
     6481 atttctcatc acgaaagaat taaaagaaac acttggcgtg gaagaaatcg tcgtgatcga
     6541 agacttagct ttgatctctg tcgttggggg tccgcataaa gaattgattg gattatctgg
     6601 gaaagtgtta tcgatattga acaaattaga aattcgcaca tcgatcttat cacaaggagc
     6661 acaggagttg aatctgatca tcggtgtacc gaataatcaa tatgaaacag tagtgaaggg
     6721 tatctatgaa gggatggtga atacggatgc tgaaagaata tcgaatggca ctccatcaaa
     6781 ttcctgaaat cgggtttcat gaattcaaga caaaaaaata tttatatgaa caagtaaaaa
     6841 acagcgaggc cgttatccat gaaatcggcg aaacaggact actcctttat tttgataatc
     6901 agcaagaaga aacgattgct tttcgaacag atatcgatgc actgccagtc aaagaagaaa
     6961 cgaatctctc ttttgcttct aagcatgaag gttatatgca tgcatgtggt catgacggac
     7021 atatggcgat gcttttaggg ttgactgact ttataactaa acacattgag gatcttattt
     7081 ataatgtagt actgatctat cagccatcag aagaaatttc cggtggcgca caaagcgtta
     7141 ttcagtcagg attgttggat aaatatgaag tacaagcaat atttggcttt catctatggc
     7201 ctggactagc taagggagaa gttttctctc gaccaggacc tttgatggca caaagcagtg
     7261 aaacagacat tatcattaaa ggccagtctg cacatattgc ttctagtgaa aaaggaatcg
     7321 atagtctgga ggcagctgta cgatttttga agaatgttta tgattttgac cggcaattac
     7381 cggcagagtg gaatcatcta atgaaatttg gccaaatcca aggtggaacg atccgaaatg
     7441 ttttagctgg agaagttatt gtatccggaa gtatccgttc ttacagttca gatatgcaaa
     7501 attatttgaa gaggggatta gaccatttag cagaacagtt tcactctaca gctactattt
     7561 cttttcgcta taatgacggc tatccagctg taatcaacca tagaggctta tatgcagcac
     7621 tgttagattc aaacaaacta catgaattag atgctccagt tcttcaagcc gaagattttg
     7681 gcatatatac gcaacaatat ccatgtgtat ttttcttttt aggtgtcggt gatacccctg
     7741 ctttacatga tccgacgttc gattttgata tggatgtttt agaaaatggt gtagaatggt
     7801 acaaaacgat tctttttaca gaaaatcttc ttgtaaaaat cgatagcagt tcaaaagagg
     7861 agcgagtaaa taatgatgaa tttatccaat cgatttagtc ctaggctttc tagaatagaa
     7921 gtatcaaaaa tacgacaatt cgatcagcaa atatcgtcga taccagatgt gatcaaactc
     7981 accctgggag aaccggattt tccaactcct gaacatgtca agcaagcagg aatagcagcg
     8041 attgaagaag acttttctca ctataccgga atgagaggat tagaggaact gcgggaagcg
     8101 gcttgtatat ttcagcaaca gcgttatgga ttaacttatg atccgcaaac ggaagttttg
     8161 acgacagttg gtgcaacgga agcgattgcc tctgcgttgt tatctgtttt ggaagaagga
     8221 gacaaggttc ttattccagc gcctgcttat tcaggatatc agccattagt ggaactagct
     8281 ggtgctgaat tgatcccgat agatacttct gacacagggt ttgtctgtca gccggaacaa
     8341 tttgaacgag catttgaaca atatggatca gctgtcaaag cagtcatttt aaactatcct
     8401 aacaatccta ctgggacgac attgtctgca aaacaattga aagcaatcgc tgaggtttta
     8461 aaaaaatatc cggtatttgt tataagtgat gaagtatatg ctgaattgac ttatagtgga
     8521 acgcatatgt ccatagctac ttatcttcca gaacaaacaa tcgtgataag cgggttatcc
     8581 aagtcacatg cgatgactgg ttggcgagtg ggctttattt ttgctcaaaa agcccttatt
     8641 gacgaactaa ttaaagtcca tcaatatctg gtgactgccg ctacaacgat gtcgcaaaaa
     8701 gctgcggctg aagcgctaat aaatggggtg aaagatagcg agaatatgaa agagcaatat
     8761 caaacacgaa gagactatct ggtccagcaa ttggcgcctt taggatttga agtgacccag
     8821 ccaaacggtg cattttactt gttttgcaaa ctcccagctg ccattcaaac tgactcttgg
     8881 cagttttgtg tagaattagc ggaacaggca aaagttgcgt gtatcccagg aattgcattt
     8941 ggcccagaag gagaaggtta tatccggatt tcttatgcta gcagcatgga aaaattacat
     9001 gaggcctgca aacggatcaa agacttttta acagaacagt gattcataga ttttatcaaa
     9061 aaaacaatcg ttaattttgc ggtatattct gcaaggagac gattgttttt taaatttagt
     9121 tcaaatgaac gcgtttcttt tgattcataa ctaatgtaat gttaagttta aattgtttag
     9181 aatgatttta aaagaatatt caaggaggtt tcatcaatgg aaaaaatcaa agttgtcatt
     9241 gtaggtgcat ctcatggagg gcatcaatcg atcttggaac tattgacacg atacggtgaa
     9301 aatgtagata tcacgctatt tgaagcagga gactatattt ccttcatgtc atgtggtatg
     9361 gagctgtatt tagaagacaa ggtaacagat gtaaatgatg tacgtaattt cagaccagaa
     9421 aactttccgc aatccaatgt tcatatttta aataatcacg aagtaaaaac gatcgatgcc
     9481 gataaaaaaa cagtaacggt tactagcgca tcagataacg aaacaaaaga atatccttat
     9541 gacaaattga ttttgagttc tggtgtcaaa ccaaacaatc tgccagtacc aggagcagat
     9601 ctagaaaatg tctacttgat gcgtggatac aattgggcaa ccaaaatcaa agaaaaatta
     9661 aatgatccgg ctattaaaaa aatcgctgtc gtaggtgcgg gttacattgg gatcgaagca
     9721 gcagaagcaa gccgaaaagc tgggaaagaa gtcgtattgc ttgatgtgat cgatagacct
     9781 ttaggcactt atttagatgc tgagatgaca gatattttag aacaacacct aaatgaaaac
     9841 ggtattgaag ttttgacaaa tgcaaaaatc gaagctttta ctggtaacgg aaaagttgag
     9901 gcaatcaaaa caagtgaaaa agaaatccca gcagatctag tcattcaagc tgccggtgtg
     9961 aaggcaaata cagaatggtt aaaagggatc gtcgacttag atgaacgtgg ctggatccaa
    10021 acaaatgaat acttgcaaac gaatcttcca gatgtgtatg cagtaggaga tgctacactt
    10081 gcttattcta tcccagcacg caaaaaaatg ccgatcgctt tagcgactgt tgcacgtaga
    10141 gaagcacgtt atgtagtaaa acatcttttt gaagaagtac caaacaaacc gttcggcgga
    10201 gtcgtaggtt cctctgcatt aagtgtcttt gactatcatt ttgcagcaag cggattgaat
    10261 agctttacag cagcaaaagc aggagtagat gtacgagtgt cttattatga agatacgtta
    10321 cgaccaaaat atgtaccaga agaacaagga aatccaaaag tgtccatcca attattctcg
    10381 gatccaatga cccatcaatt gctaggagga gcagtattat ctacctatga tgttacaggt
    10441 caaggaaatg tattggcact agcgatccaa aacaaactga ctttagaaga actagcagaa
    10501 gctgacttct tcttccagcc aggctttgac cgacaatgga gcatgttgaa tctagccgct
    10561 caacaagcgt taggagaaga accatttgta gaataagaaa acgaaatatt tgaaaagagg
    10621 ttgtgaaaaa gctgccttgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat
    10681 gtttttaact tggctggact tattaccgca gatgcaagct tttgaacacc gtttattttc
    10741 aaaaaaggga ctgtgacacg atttgtgtac cgacccccaa aagttagacc aaaaaatcta
    10801 acttttggag gtcggtattt tttatggtta aatatacttt tgaacttaaa ttaaagatcg
    10861 ttcatgatta tttggatggc aaaggcgatt ggaccgtaaa aatccagatc aagagattga
    10921 agcggaaatg cttaaaattc gggagaagca caaggattat ggctgtttac ggatgaccaa
    10981 tgaactaaga aaccgcggtt tctctatcaa taaaaagaaa gttcagcgct tgatcaagaa
    11041 gctaggaata aaagtaacgg catacacgcg taaatcacgt cgctacaatt cttatcgagg
    11101 aaaagt
//
LOCUS       DAFCHQ010000084        10851 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000084 DAFCHQ010000000
VERSION     DAFCHQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.084"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(104..481)
                     /locus_tag="LNC71_002338"
     CDS             complement(104..481)
                     /locus_tag="LNC71_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467361.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(781..1662)
                     /gene="galU"
                     /locus_tag="LNC71_002339"
     CDS             complement(781..1662)
                     /gene="galU"
                     /locus_tag="LNC71_002339"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBK6467362.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1705..2724)
                     /locus_tag="LNC71_002340"
     CDS             complement(1705..2724)
                     /locus_tag="LNC71_002340"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBK6467363.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3357..4361)
                     /locus_tag="LNC71_002341"
     CDS             complement(3357..4361)
                     /locus_tag="LNC71_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6467364.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4374..5423)
                     /locus_tag="LNC71_002342"
     CDS             complement(4374..5423)
                     /locus_tag="LNC71_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6467365.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5440..6261)
                     /locus_tag="LNC71_002343"
     CDS             complement(5440..6261)
                     /locus_tag="LNC71_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6467366.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6248..7027)
                     /locus_tag="LNC71_002344"
     CDS             complement(6248..7027)
                     /locus_tag="LNC71_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6467367.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7044..7514)
                     /locus_tag="LNC71_002345"
     CDS             complement(7044..7514)
                     /locus_tag="LNC71_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6467368.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7526..7945)
                     /locus_tag="LNC71_002346"
     CDS             complement(7526..7945)
                     /locus_tag="LNC71_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBK6467369.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8026..10803)
                     /locus_tag="LNC71_002347"
     CDS             complement(8026..10803)
                     /locus_tag="LNC71_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6467370.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT         3102 a         2318 c         1783 g         3648 t
ORIGIN      
        1 cttttatata agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag
       61 tttatccatt attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg
      121 ttcttctact ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc
      181 tgcctatccg agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa
      241 aaaataaagc aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag
      301 caaccccaac ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca
      361 tgcgaaacat gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc
      421 cttccatcag ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca
      481 tagtgaacac acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc
      541 ttctggcttg gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa
      601 attaaaccat tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg
      661 ttttccacga ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg
      721 cacaagctgc cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag
      781 ttaactttct gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc
      841 aatttccgaa tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc
      901 tacgtcgtat ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt
      961 ctgaagtgca tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat
     1021 ctcaaatatt ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt
     1081 tcccatttca ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt
     1141 tttaggatca ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac
     1201 agcggaagct tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc
     1261 tttcatcaaa tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc
     1321 ttgaagcacg gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac
     1381 ttgagtcgtt tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt
     1441 ttccaattcg atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac
     1501 gatcaagatg tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg
     1561 tttatccacg attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt
     1621 tcctagccca gcggcaggaa tgactgcttt tttgaccttc acttgctgtt cctcctaaac
     1681 gacgattaac tattgatcat ctatttaacg aagagaaaat tcattctcca tttttccgtc
     1741 acgtaacatg atttcttttg ctgctttttt gatatcttct ccattataca aaacattata
     1801 gattgtttga gtaattggca tctcaacacc tagctgttga gctagctcta ctgctgcttt
     1861 tgtcgttgcc acaccctcaa cgaccattcc catgttttct agaacttctt ccagcttatg
     1921 tccctgtcct aacaagttac cggcacgcca attgcgagaa tgaacacttg tacaagtaac
     1981 gatcaagtcc cccacaccac tcaaaccgat aaatgtcaga ggattggcac ccatcgcgac
     2041 accaagacgg ctgatttctg ccaatcctcg agtcatgatt gctgcttttg catcatctcc
     2101 gaatcctaat ccatggatag cgccggcacc aattgcaatg atatttttca gtgctgcacc
     2161 agtttctacg ccaatcacat cattatttgt ataaatcctg aaataatcat tcatgaacag
     2221 tttttgtaca taggctgctg ctttttgatc aatacttgca gctgtaattg ttgtgatatc
     2281 atgtactgcc acttcctcag catggctagg accagataat acaacaattg cctgtctttt
     2341 ttcttctgga atttcttcag ccaaaacttc tgagattcgt ttatgcgtat cttgttctag
     2401 tcctttactt gcatgtataa tgattggctt agtattcatt ttaccaataa gttcctgtgc
     2461 gactgaacga atcgcttttg ttggaacaac aaataaaata gcatctgcgt tatccactgc
     2521 ttctgccaat tctttatatc cgataatcga ttctgggatc gtcaaatcag gtaaaaaatg
     2581 ctgatttgta tgatacttat tgatttcatc aatctgttca ggtacatttc cccaaatacg
     2641 gacatcatgc ccgttttctg ccaatgtttg tgctaaagca gtgccccaag atccaggacc
     2701 aagaactgcg attttttgtt tcatggatat tcctccaaat aaataattga taatttccat
     2761 tatagtatac atttcttaaa gttgggacta atttccattg tttttaagtg tttgtttact
     2821 tcttttatcg tttttacttg aaataatcaa tgatatgtta caatagtcta cattttattt
     2881 ttacactcgt tatgctttgc ttgttcctaa gaggaagcag ttttgacaat gttactacca
     2941 gcattactat taggaggtta ggaaacttct cctagtgagt attgatttaa aatacataag
     3001 agacagattg aacatttcta aatctgtcag gtcctgcaac ggacagattg tctctgttga
     3061 cggttgacat aggaactttt gttttagtca acattctgga tacaaaacgc tatgtaaact
     3121 ggcgcaactc gttgtacttg gacttttact acggatccat gcaacaacta aatagtacta
     3181 ttgcataaca aaaaatggtt ggcacccaca tgtcccatta aacagattat aagttgaact
     3241 gtagataatg caaaaaatgt gttttataac gtaattgatg ccaatatttt aaaaaaataa
     3301 agggagcagt tatcgctgct ctctttattt tttatattct ttttatccag tctatcctaa
     3361 taatcaaact gacgatagta gcggcgtatc tctaatggat ggcagttcaa ctgctccaca
     3421 tgagcatcga tgcgttgtgt cactacatgc atcaataaga aagacaaaac acgtccgcga
     3481 tatttttcac tgataccttc tactggatag tcttttgtgt ctatcaatgt ataattgctg
     3541 caaatttttg gcaaaagttt agccacacgt tcagataaca ctctctgttc atcttcgcct
     3601 aaaaataatg tgactggggt tgtttcttct acgatctcta acgtcccgtg aagaaactcg
     3661 gcagcatgga tagatttcga tggaagccag ctttgttctt cccagtaaca catggcatat
     3721 gaataaacag ctccccattg attccctgct ccaataaaat aatgcatact atcacgtcga
     3781 tgtttttctg caaatgataa gccaaatgca tctgcttttt tctctgcttc cactaaccct
     3841 gttggtaaat aacgttcgag ctgttcatag tattctgaat aagcttcgaa ttctccattc
     3901 aaatacatca atcgatctgc tgccataaaa aatttgattt gttctgttcc aggagctgga
     3961 taagtgacca caaaatcgca tagctgagac aacttactgt tcgctttatc gataaatcca
     4021 atcagtgtcg ctcctacttt cttgatttgt tctactgctt tcactacttc ttgagttgta
     4081 ccggtaacag aggacaaaat aacgatagaa tcttttgtta atcttttgtt tccagtagtg
     4141 taatattctg cagcatgctg tacaaagaca ggcaagttgc ttttcccatt catataagtg
     4201 acagcttgca tcgaggaagc atacgttcca ccgattccta gataatagac agcgtcaaat
     4261 ttttccgcat aaagtttatc gatgatttct tctacttgcg gacgtaaagc tagtgcgcct
     4321 tttattgctt tgatttgttc ttcttcattg aatttcagca tgatttccac ctcttataat
     4381 ttacttttca agactgtatc aaagtcttca aaaatctttt tgttcaagtc aataccttta
     4441 gctactgcgg ttttataagc gaatagttga aacggaacga caagcgctaa tacagacaga
     4501 tattcatcac attctattgc taatccaagt gttttctcgt ttcttgcttt ttttgtggta
     4561 atggtgagag tctctccaac gtaccgactc atatatcttt gcaacttttg cgaacgttcc
     4621 ttatttacac tttccccttc aataaagaat agcaaatgcg aagcatctgc ctccaggtat
     4681 gggccgtgca tataagcttc taactcaaat ccttgtgacg gaacgcggat tgtttcagtc
     4741 aatttcgtct ctgcttcttt agctgttccc cagtttggac catatccaat ggtgacgaaa
     4801 cgctgtgcta aacgaaataa actttgatat ccatcaaaaa attcttccgt tttttgaata
     4861 atcgctggta aatggttgat tattttgcgc aactgttgca tgtagtcttg ttctattttt
     4921 ttgctgattt tatttttact tattccaatt cctatcccta agagtagtaa ctgtaaaaca
     4981 gtcgcagaat agccttttgt aacgaagcct actgattcta ccccggcacc taaatcaata
     5041 atttgtgcag cttctaaagc aagtggactt tgcacgtcat ttgtgatagc aacaacaggt
     5101 aacccttgtt tttgtatcat ttttactgct tcaacagtag aagcactttt cccgctttgt
     5161 gacacagcaa tcacagtatc gattgaagga gaaagttttc cataattgaa aaaataaaaa
     5221 ggttcttgaa tatcgacagt aagatcacca taagattcca atgctggctt cgctgctaaa
     5281 caagcgttat aagatgaacc cgttgctaag atcagtaaat tcactgtttt tttcatatgt
     5341 tctcttgtcg aaaaatcatt ctttttcaag atcgtactta gtacttcctt ttcttcatag
     5401 atataatctt gcatcgtaaa cattttctct gctccttttt taaaaaatcc ctatccacga
     5461 accaaaaatt ccaaagaaag ctagtccgat caagatcgtt aatggtttca cttctttctt
     5521 caatagataa aacactatgc caaatgctcc aagacataag atatttggaa cgatatcatc
     5581 aaaaatactt tgaacagtca cagcattttc tcctgaaccg atttttaaag gaatattgat
     5641 ggtgatcatc gtggcagtca ttgctccaac gaccatcagt ccaataatag acgcgcctaa
     5701 tgtcaaactt tccatcatac cgtttgcctg gatctttttc aaaaaacccg ttcctaagcg
     5761 atatccccaa cctgttgcta gatagcgaaa cagaatgtgc ggaatattga aaatcaaaat
     5821 gaacaagata gggcctaaaa tatttccttg catggctaaa gaggtaccca cacctgtggc
     5881 aatcaatcgc aaagttcccc aaaagaaaga atctcctaac cctgcaagag gtcccatcaa
     5941 agatgttttg atactatcga tcgcagtcac atcaaattca ggatcatcct tattttcttc
     6001 ctccattgca gccgtgattc ctaaaatcag tgtaacgata tgtggggtcg tgttaaaaaa
     6061 ctctagatgc cgtttcaaag ctgctgccat ttcttctttc gactgatata actttttcaa
     6121 aatcggaatc attgcataaa cataagccat attcatctga cgttcataat tccaagaaaa
     6181 ctccatttga aaagaacgcc aaaatacgtg atttagctct tttttcgtta ttttgctgtt
     6241 tttgatctca gaattcttca tcttcttcta cctcccctgc agttgtttga agtggtagtt
     6301 gacggacatt cataatattg actaccacaa ctgctaaaat cgcaccaaaa atagctactc
     6361 cggtcacagg aatctctaag taagaagcta tagcaaaacc taagaaaaaa tatggtgcaa
     6421 cttgtttgtt gatcagcaaa cgagcaagca tcgcaaaacc aagagctgga atcaaccctg
     6481 tagctactga caaccccttt tgaacgaagt caggaatcat atttaataga tttccgatcg
     6541 tattgcttcc caccatataa gaaaccatta ccacaacacc aagcatcaag gaaagaccga
     6601 taccggcaag caagtgcata tgctcgactc ctttataatt tccttcttct gcataattgt
     6661 ctgctttatg gttcataatt ggaatcaaaa gccctaagta aatatttttc aatatcaatg
     6721 tcaacgtagc aatcggcaaa cctagtaaaa gcgcggtttc tgtaccggct ccagctgtaa
     6781 tagcaaaagc cgtccctaaa atcccaccgg tcacaacatc aggtggaatc gaagcaccta
     6841 ctgagaacga tccgataaat gctaactcta aagttgcgcc cataatgatc ccagttttta
     6901 catcacctaa cacgattcct gtaaacagcc ccgtcacaat cggacgagaa agcaacgatg
     6961 tccctagtgc atattctgat tgtgcgacaa atgcaacaat tcctaataag actgcttgta
     7021 caatcattga ggttcttcct ttcttacaaa acttctctta catccacttt tttatctgct
     7081 ggtacttgac ggatttctac ttctatttgt ttgtcaatta atcgtttcaa gcgttcctcc
     7141 tcttctggta aaatatttac cgcttttccg atgctgcgag taccaggttt tgccttgatc
     7201 cctcctaagt tgatggtttt gatttctgga tacgcttctg ccagcttata tgcatcctcg
     7261 atcgattcca ctacgatgaa taacttatat ttatctgtca ccccgctttt caacgcggca
     7321 atcgagtcat caattgattt gatcactagt ttgactcctt gtggcttggc taatttgatc
     7381 gttgtctttc gtatctcatt cgtaggtaca tcatcattag caatcaaaat acagtctgcg
     7441 cctattgttt gtgtccatga aaaagctact tgtccgtgta ataaccgatg gtcgactctt
     7501 gtcaatacaa tcatttttct tcctcctaaa attcgtcttc ttctattttc tgattgagcc
     7561 gctcgttaca aaactggatc attttttttg aatcagttag cacctgctgg atcaaaacag
     7621 ttgtctctac ttcagcgtcc aaccgagata ctagctcaat caatagtggc aaattcaatc
     7681 cggcaacgag atagacatga tcccgattga tatattgtaa gaattcgttg ttgacacttc
     7741 cgccaaaaag atccgtcaga acaatgattt ctttatcttt gtacctcata aaaagctgcc
     7801 ttactgtttc cgctaaatca aaattctcat cagtatagca atcgaataca gtaatagaat
     7861 gatttttccc atagatcagc tctaatgaat ctaatatccc tgaagcaaat tttccatggg
     7921 aagctaagat aatttctcta tccacttttt catctcctct tttcacttac acatactaaa
     7981 aaagcaagtt gcatgccaaa attagtggca acaacttgct tatgttcaaa attcttcttc
     8041 tatcgagaga aactctgtat cttcactcaa aatatcatag atataggcaa tctcagaatt
     8101 ggggatcatg acactataat gctcttcaat gacactaaac gcagatttaa tagcagataa
     8161 cccttctttt tgacactgct tccatttctc taatccttga taggtttcga ttggcatttg
     8221 tcggatcaat cgttcaatga gacagctcac atggacatac aacgctaatt ttttgggatt
     8281 gctgatgctt cgattcaact gcacctctaa ttctctcata aaatgatcga tttctcccat
     8341 caccttctct gtatctagaa tagtcacgga atccagtact ttttccaatg aaaaattacg
     8401 aatgatgcgc tgattgaata tttccttctc tgaagcattc atagtagtgt ccaaccattc
     8461 agctagtaca gacatctttt cccccgagat gaggtcttct aatgaaagat agtcgatatt
     8521 ggtgatatca ggatctgtgg tgccaatcat tcctacgact tcgtatatgg aaaataatgg
     8581 gtcactgttt ttcttatctt ttaattgctg atattcatat gggatgattt tcaactgaca
     8641 agccgtaggt aagctttttt ctagcagttc acttaagtgg accgccgtac cgattccagt
     8701 gaagcaagta gtaatgagtg ctttgggttt gttttcctct ggatagagca gtcgccattc
     8761 tggctggatc tgtcgaacag tctcttctgc tatctcttct aatgatacat ttttttgcag
     8821 gcattcccct actgtgattg ctaaaggtgt cgtcacatta ttcaaaatca aaagcggtac
     8881 ggataactgt tttttaaaaa actgatggat ttctttcaga gatcccatgt ctactaatat
     8941 gatcaatcca tttgaaacat cgctgttttc gcaataatca aggatttctg aagcaatctg
     9001 ctgtggtgtg acatctagtg gcatatcaaa tgattcaaat agatcctctt gtaataaacg
     9061 atttactaca tttgccatac tgctagcagt tgcataaccg tgagcagcaa tcatcgcttt
     9121 tggcagtcgt ttttcttttg accatttcgc tttttttaga tagatcgtca gtaaaatccg
     9181 atccatactt gtgtcttcaa tatctaattt gatcttactt aattccagaa ttcgttggac
     9241 atagtgataa ctagatggat aagactgaga aacttgttgt tctaattctt tgattagttg
     9301 gataatatca tgttcttccg gtaaccattt gatagtgctt cgctgataga gatagtaact
     9361 caaggcatag acactgtttc catcgaattt gatttgatag gcagtttcca tttgtcgaaa
     9421 ggtctctcgg atagattgtg tccaataaac cagcagctcg tgtttttctt gtctactggt
     9481 ctcaaacagc aaaaaatcaa ataattgaat cacttcttgc tttaattttc cttcgcaaga
     9541 atatagcaga cagttatttt tcttgaaatc aattaggatt tgctcaaaac ttttaacgat
     9601 tcgctgctgt tcgggctgtt gcttctccat taattgctct aaggtctggt cttgcgtaat
     9661 atagattcct tgctgtgctg tatttttagt aataggctgc gcaagtagtt ctttttgcaa
     9721 atgatggatc gtgacatgga tttcaggctg ttcacgctgt tcagcatatg cttttgctgc
     9781 tgaaactttg acgatatttt tcagctctcc tatgtttccg gataatttct gacttgttaa
     9841 aagtgacaaa gctggtccac tgactgacaa agggctcccc atttttcttt gttcttcgag
     9901 aaaaaaagag taaatcagtt ctagccgttc attacgacta cgctgattaa gtgcaggtag
     9961 ttcgattcgg ataggtattc ttcgaatgaa agttgttaag aaatgactag taatctcttc
    10021 agtcgtagca aaaaagagac gtgttttgat ccggcgtgac aaattaggtt ctcccatacg
    10081 ataaatcaca ccttgatcaa gataggtgaa cagtttttcc tgaccttctg gtgttaagcg
    10141 atgtacctcg tctaaaaaca agatcccacc atctgctgct tcaaaagccc cttttttatt
    10201 ttcttctgca ccagtgtacg ctcccttaac atgtccaaac aaattgctag tcaaaagttc
    10261 aggattatct gcatattgtg cacaattaaa agtgatgaat ggtgcagaat cttctagcag
    10321 atcatgaagc agacaatact gatagaccaa attaactaaa taacttttac ctgtgccact
    10381 ttcgcctgta atcaaaagtg gtaatccgcc atctggatag taaagagctg ttttgatttg
    10441 ttcgatactt tctttcaaac tggcgtcgta tccgatcata aaagagaata agtcttttgg
    10501 ttgcgcgaaa aaaggctgtt cctctttgag atcatcgata gaaaaataaa aattcgtgcg
    10561 taacttaaaa aattgttttt caaaagcttc tttgtggaaa taaatcactg gtcgagaatt
    10621 gattttgact agtagttgct catcatttaa ctggttcaaa taatgactga ctgtgtttct
    10681 ttttacccca aaatgatcag ctaacctttg tgctgtaaag acctcgttca tatgttctaa
    10741 atcgaaaaag gctgtctggt tctctaagta acataaaatt tccttcttca acaacttatt
    10801 cacccctcct ccatcataga aaacggattc attttgtcaa taaaatcttt a
//
LOCUS       DAFCHQ010000085        10760 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000085 DAFCHQ010000000
VERSION     DAFCHQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.086"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..1042)
                     /gene="istA"
                     /locus_tag="LNC71_002348"
                     /pseudo
     CDS             complement(59..1042)
                     /gene="istA"
                     /locus_tag="LNC71_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="LNC71_002349"
     CDS             complement(1437..2279)
                     /locus_tag="LNC71_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBK6467371.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="LNC71_002350"
     CDS             2604..3020
                     /locus_tag="LNC71_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6467372.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="LNC71_002351"
     CDS             3017..3487
                     /locus_tag="LNC71_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6467373.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="LNC71_002352"
     CDS             3501..4274
                     /locus_tag="LNC71_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6467374.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="LNC71_002353"
     CDS             4288..5100
                     /locus_tag="LNC71_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6467375.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="LNC71_002354"
     CDS             5154..6119
                     /locus_tag="LNC71_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6467376.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6255..8978
                     /locus_tag="LNC71_002355"
     CDS             6255..8978
                     /locus_tag="LNC71_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6467377.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8991..9623
                     /locus_tag="LNC71_002356"
     CDS             8991..9623
                     /locus_tag="LNC71_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6467378.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9828..10055
                     /locus_tag="LNC71_002357"
     CDS             9828..10055
                     /locus_tag="LNC71_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBK6467379.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10124..10390
                     /locus_tag="LNC71_002358"
     CDS             10124..10390
                     /locus_tag="LNC71_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6467380.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10380..10649
                     /locus_tag="LNC71_002359"
     CDS             10380..10649
                     /locus_tag="LNC71_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6467381.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3566 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcagatgcag cgattaaatg actatcttta tcaactaaca ttgcttttga
     6301 aatctacgcc ttcagataat tattcagctc gcagtatttc gcaagctcta cagttgaata
     6361 gaagtacaat cagtagctac ttaaatgaag gtgttcgtga agggcttttc ataaaagtaa
     6421 aaagttaccc ggttttattt ttacatagaa cagctcttga agaactacat atcacactga
     6481 ataactccga aatagaatca attgaaagct tgttaacaat ttctcaaaaa cccgcattag
     6541 atcaagtaat tggctctaaa ggaagtttga aagaagcaat tgatcaaatt aaaacggcag
     6601 tcttatatcc tggaaaaggg ttgcctttat tattgatcgg cgctagtgga tcagggaaaa
     6661 cctttctagc gaataaaata tatgaatatg cagtagaaga aaaggtcatt cacacatcgg
     6721 cacctttcat agattataat tgtgcacaat atgtgagcaa tccagagtta ctatcctctg
     6781 ctttatttgg ctacacgaaa ggagcattta ccggagcaag ccaggagcat actggtttgc
     6841 tagaaaaagc cgatggagga gtattgtttt tagatgaagt gcatcgctta tctgaagaag
     6901 ggcaagagaa attatttaca tttatggata cgggtgaatt ctctcctatg ggggataata
     6961 gtattcggaa aaaagccgat gttcgattag tctttgcaac aacagaaaat atatatacga
     7021 ctttcttacc aacttttctt aggagattgc cagttattgt taatctacct agatttcaac
     7081 aacgtccgtc gtttgaacgt ctgtcattga ttgatgaatt ttttgtttct gagagccaaa
     7141 ttttagctaa ggaactatct gtttctgatg cattgattca tttcttaatg aatagtgatt
     7201 ttgaaggtaa cgtggggaat attaaaaata ttatcaagta tgcttgcgga aacgcatata
     7261 tccaccagaa gagtcagcaa acaattcaag ttaaattatt ggatatgcct ttagagtaca
     7321 gttataaatt caaggagcaa ctaaataaac ccagaaagaa aagagcagat cgaatctact
     7381 atccagaaga aacacgacaa gtgcaactag aaagaaacaa tcagcttttt gttcaatttt
     7441 ttgaccaatt acttactgga ttctctgaag tgattgaaaa taacaaggat attaaactat
     7501 ttttagaaga aatggttacc aaggtaacac aagtaatgga tacgcttatt tttcaagagg
     7561 attacgaaaa agagcagtct ttatacgcca ttctaaacta tcatattcgt caaagtattg
     7621 attttatgcg agaaagctac gggtttgaac aagatggtaa ccgggtagtc gctttggcca
     7681 actatctata tgtcaaggat aatcaagtga tttttgaaaa tagtcttcaa gaaaagaaaa
     7741 ccaaagatca gcttaacgaa ttttttgcta atgttcttga aaccccttat tggtatgcaa
     7801 aaaaaatctt aagcctttta tctgagtgtt tagatcaacc tgtgtatgaa gaggacattt
     7861 tctttattac gctatatttt tatagtttga atgtcacgaa tgttaaaaca ggtattaaga
     7921 gtattgtgct agctcatggt tattctacag ctagtagtat ggcgaatgtt gccaaccgta
     7981 tgctgaataa gaacgttttt caagcttatg atatgccttt agatattaca ttagagaaaa
     8041 ttgaagcaaa attgattcaa ttccttggag attatcgaac agattcaggc ttgattttat
     8101 tggtagatat gggttcacta aaccaactag gcggtttatt agtgaatcat ctaaatggac
     8161 cactgttaat cattgatcat atcaatacac cgttattatt ggaagtcggc caacaagtat
     8221 taaacaataa aagtatcacc gaaataggag cagaaattga aacaagtctt actttcaaaa
     8281 agcagttact acttcccaag aaaaagaaaa agaaagccat cattacttgt tgttatacag
     8341 ggattggtag tgcgacccag attcaggaga ttttaacgaa atgcttgaaa gatgcagtta
     8401 gagaattagt aattattccg tatgattaca aaaagctgtt agaaaacaag aattttgaga
     8461 ctccttttca attatacgat gtcatcatga ttgtaggtac agaagatccc aaaattaatc
     8521 agattccata tattggatta gatcgcctga ttaatggggg aagcattggc gaatttgttc
     8581 attacttgga aaaagagctg actattgaag tgaacgattt acaaaaagaa ttaattttca
     8641 atttttcaat tagtaaaatt gtcgaaaatt taaccatttt ggatccagat aaggtgctat
     8701 cttttgtaca aaaagcaatt aaagaattat cgaagttatt aaattttgat ttttctaata
     8761 atcagctgtt tttgttgtac cttcactgta gttgtatgat tgaacgaatt gtacgtaaag
     8821 aaagtgtgga tgagcaagag gacattgaag aatatatgca agcaaatgga gaaataatgg
     8881 ataaaattca tagcgctttt tttgagattg aaagggaata cacaattgaa ataccagatt
     8941 tagagttgcg attattgagc gatattgttc aaggatagga gtgaattcga atgacaatgg
     9001 gagtaatttt tgatatggat ggggtactca tagatagcga gacattttat tttgagagaa
     9061 gaatgaactt ctttcaagaa ctggagatag atccaggatc aaaccagatt gatgattatg
     9121 tggggaaaac agaagctgca atatgggaaa cattagttcc tgaggacaaa tcattaagag
     9181 aggaacttta tccgaaatat gtagattatc gaaaaacgca tccaattgat ttcgctaaag
     9241 ctttacgaaa agaagtgaaa ccgttacttg acttcctcaa gaagcgggat ataccattgg
     9301 cactggcatc ttcttctcca gctgtcgaga tagagagtat gctcattcaa tgtgaactta
     9361 ctgaatattt ttcctttgtt ataagtggtg agtcattaaa acaaagcaaa ccacatcccg
     9421 aaatatattt aaaagcgaaa cagcaattag actgtgactt ctttattgca gtagaggatt
     9481 caacgattgg tattaaatca gcaaaggcag caaatatcta tacggcggca ctaaaacaac
     9541 caatgcaaat cgatcagagt gaagcagaca tacaaattga aagtctacaa gaactggaac
     9601 aaattgtttt agaaaaaggt tagtgaatta atggaaataa ggctatatat ctgtgctagc
     9661 tattttgact aaccaaaaaa acttgctata attcaggtgt agtaaaaggt gctggtaaca
     9721 ccttttacga aaatgcttcg gcagtgataa tgggattggc tttttaagtt ttttattttg
     9781 ttcgcaatcc ctaaggtcat gtgaatgatc ctagggattg ctagaatatt ttgcaacaga
     9841 accaaaatac gaatagttat caagcagcca tattgactgc ggtcaattta ggaggggata
     9901 ctgatactac tgctgccgta actggtgctc ttgcgggtat tctttacggg ggtgaagtga
     9961 tccctgaaaa ttggagaaaa tcgcttcaaa atgaccagct tcttaaaacg atcagtcaga
    10021 gattttgcga taccgtgcta tctcaaagaa tttgaataat ggatatgatg caaatcttgt
    10081 ttgtactaac gttgtattga caaaaaggat tttatttagt acaatgttag tacaaatgat
    10141 gaacggaggt agaatcatgt ctcaatcaac cattacattt aggattcccg acgatgaaaa
    10201 agaactggta ttagaatatg caaaagcaca caattcttca ttgaccgaac tttatcgaaa
    10261 tgctgtactg gataaaatcg aagatgaaat cgatctacgg actttacgga gtgcaattga
    10321 agtttccaaa gagaaaaaag agagtggtat ttctcaagag gaaatggaga aactgctaaa
    10381 tgaattataa agtcatctac atgccgcaag cccagaagca attaaaaaaa atggacagaa
    10441 accaagctaa aataattgtc gcctggatca gaaagaattt agaaaacacg actgacccgc
    10501 gtcagcacgg aaaaggtttg actggaaata agagtgggga atggcgctat cgaattggaa
    10561 actatcgaat attggctaat atttgtgatt ctgaaattga aatccagata ttctcaattg
    10621 gacatagaag tacaatatat aaaagataac cgtttttgga acgaagtaga gtttcgaaag
    10681 tcaatgtata atcttttgaa ttttgcataa taccacaggt agaaactacc cgatactatc
    10741 ggttggtttc tgctttttta
//
LOCUS       DAFCHQ010000086        10689 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000086 DAFCHQ010000000
VERSION     DAFCHQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.087"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(232..1080)
                     /locus_tag="LNC71_002360"
     CDS             complement(232..1080)
                     /locus_tag="LNC71_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6467382.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1079..2146
                     /gene="gnd"
                     /locus_tag="LNC71_002361"
     CDS             1079..2146
                     /gene="gnd"
                     /locus_tag="LNC71_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBK6467383.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2148..3149
                     /locus_tag="LNC71_002362"
     CDS             2148..3149
                     /locus_tag="LNC71_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBK6467384.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3146..3430
                     /locus_tag="LNC71_002363"
     CDS             3146..3430
                     /locus_tag="LNC71_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBK6467385.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3463..4260
                     /locus_tag="LNC71_002364"
     CDS             3463..4260
                     /locus_tag="LNC71_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBK6467386.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4274..5107
                     /locus_tag="LNC71_002365"
     CDS             4274..5107
                     /locus_tag="LNC71_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6467387.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5125..5982
                     /locus_tag="LNC71_002366"
     CDS             5125..5982
                     /locus_tag="LNC71_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6467388.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6004..6513
                     /locus_tag="LNC71_002367"
     CDS             6004..6513
                     /locus_tag="LNC71_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6467389.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6550..6951
                     /locus_tag="LNC71_002368"
     CDS             6550..6951
                     /locus_tag="LNC71_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467390.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7063..7416
                     /locus_tag="LNC71_002369"
     CDS             7063..7416
                     /locus_tag="LNC71_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467391.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7450..7893
                     /locus_tag="LNC71_002370"
     CDS             7450..7893
                     /locus_tag="LNC71_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6467392.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7910..8995
                     /gene="uxuA"
                     /locus_tag="LNC71_002371"
     CDS             7910..8995
                     /gene="uxuA"
                     /locus_tag="LNC71_002371"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK6467393.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8988..9701
                     /locus_tag="LNC71_002372"
     CDS             8988..9701
                     /locus_tag="LNC71_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6467394.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9908..10057)
                     /locus_tag="LNC71_002373"
     CDS             complement(9908..10057)
                     /locus_tag="LNC71_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467395.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10453..10653
                     /locus_tag="LNC71_002374"
     CDS             10453..10653
                     /locus_tag="LNC71_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBK6467396.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3575 a         1691 c         2314 g         3109 t
ORIGIN      
        1 aaaaaaaaga aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca
       61 agtaactcga ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc
      121 caccgactat tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt
      181 tggtagtggc tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt
      241 gatttcagaa atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta
      301 aaacatcaaa atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa
      361 agcttcagat cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt
      421 agcaagggaa gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac
      481 ggtttcagca atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca
      541 gtctttttcc gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga
      601 agttgcgata ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc
      661 gccaacaccg aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag
      721 atccttttgt tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac
      781 cttttgcgcc atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc
      841 agatagatca ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc
      901 aagttttttt gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt
      961 gctaatagga ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg
     1021 ttcttttggg ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat
     1081 gagaattcct cattttcata ataagattat cattttttcc gttctcatta atgttaaaga
     1141 tacaaggaga gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa
     1201 aatttttttg tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat
     1261 tggtttggga aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt
     1321 acaaggactc gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc
     1381 ttatcaagag atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat
     1441 gcttcctgct ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc
     1501 aggagacatt attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga
     1561 agagtttcag aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg
     1621 agcacgacat caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga
     1681 acaattattc aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag
     1741 cggacacttc ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg
     1801 tgaaggattc caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg
     1861 tgtttggaat catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt
     1921 aaacgaacat cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa
     1981 atggacggtt gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt
     2041 gttcatgagg aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact
     2101 aagaaatggg tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc
     2161 atatagcaat cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat
     2221 taaagtcagt tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac
     2281 tagcggagtt acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac
     2341 gagatttttt tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca
     2401 acaatattga tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt
     2461 tagttgaacg aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac
     2521 gcacgacaca agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg
     2581 tcggtaggac gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg
     2641 gagtaatcga aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca
     2701 aatcagattt atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg
     2761 aacaatcaga tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt
     2821 ctgataaaga agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg
     2881 ctctagttga tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg
     2941 ccacagatgt tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac
     3001 aagtagtgat tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg
     3061 aaaaatgtgt cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc
     3121 agacaaagag caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa
     3181 aacaggccct caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt
     3241 gaaatcatac ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc
     3301 gctacctaaa ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc
     3361 tggagaatgt agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc
     3421 gatttcgtag ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat
     3481 cttgcaagaa tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca
     3541 cctcttgctg tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac
     3601 agacaagctg ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat
     3661 ttcattgccg ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa
     3721 caagattttt tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc
     3781 aaagaagaga ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg
     3841 acatcggagt tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga
     3901 ttaggagata aatacgcaag aaatgtggca gcacatgcta tgcatgacgc tatcagtaaa
     3961 tatattccgg aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat
     4021 caactggcac tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa
     4081 ttagaactgc cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata
     4141 gataaaatgg ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta
     4201 acacagaaat cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga
     4261 aggaggagga cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt
     4321 agccgcggtt atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc
     4381 tttgatgact ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt
     4441 tacagccgct ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc
     4501 tgaaccatct attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc
     4561 agaagcagcg attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt
     4621 tcgttttttc atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa
     4681 tacaaaagct atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt
     4741 cgttccgctt gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa
     4801 agctttagag ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc
     4861 aatcggtatc gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt
     4921 ccttgcttat tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc
     4981 catttttggg attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca
     5041 gcctgccaca agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg
     5101 attttaattt aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct
     5161 gaagaaatca cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa
     5221 attcctcact cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc
     5281 ttgaaaaaac tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg
     5341 ttcttcaaca ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg
     5401 gaacagcaaa gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg
     5461 atgtataaca cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat
     5521 tcaggaaccg tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt
     5581 cctttaggcg cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta
     5641 tttttcgcaa gacaagcttt cactatgggc cgtaatgcta caggcctaaa gcaaaacact
     5701 ggtgttcaga cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt
     5761 gcgggaaact atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt
     5821 gttatccagg aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg
     5881 ggtgtgtact tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg
     5941 actggaatat taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat
     6001 aagatgggtg tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg
     6061 gtaacgttat ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca
     6121 aaagataaat ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca
     6181 attgtcatta caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac
     6241 aattgtattt tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc
     6301 cccatggaag aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta
     6361 gtgacgaaat ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa
     6421 caatatggcg taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta
     6481 aaagatgtat tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag
     6541 ggcgaaaaaa tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt
     6601 taccgtaagc gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat
     6661 atttacagta tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca
     6721 aaaaatattt atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat
     6781 acacccgttc atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa
     6841 acaatctact ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg
     6901 atgatatata acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc
     6961 actgtactag catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct
     7021 agcagacaaa cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt
     7081 acgattgatg atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag
     7141 ggcttagaca gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct
     7201 gttggggcag atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg
     7261 ctgaaacaag ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa
     7321 aagcaagcgc agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg
     7381 caaaatgcca atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg
     7441 agaagactaa tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa
     7501 gatgcggcgg aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct
     7561 ggggaggaca tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca
     7621 gcagaaccca tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc
     7681 ttggcagtca atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg
     7741 aatctgccaa tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa
     7801 gcggtggaac aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg
     7861 gaagaaaaaa aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg
     7921 atttagatgg tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc
     7981 gggaataaat gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat
     8041 ttcagaaatt gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat
     8101 tgaaagtgta gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat
     8161 tgataactat atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta
     8221 tagcttcaaa ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg
     8281 aagctattct cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta
     8341 tcaattgata cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt
     8401 gaaaaaattc gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga
     8461 aaatctgact tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat
     8521 ggctatccat ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa
     8581 tcttggcgac ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct
     8641 gtgcacagga tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt
     8701 aggttcgcgc atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt
     8761 caaagaaacg gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc
     8821 gttggtgcaa gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg
     8881 tgaagtggct atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca
     8941 aggtctatat gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca
     9001 agctcttcat ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg
     9061 cttatgtaaa atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac
     9121 taactggcca aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg
     9181 ttccatatat caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag
     9241 ataagagagt atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag
     9301 gaatccatac aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa
     9361 tggaaaatct atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa
     9421 agccggaacc agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag
     9481 cacttgtatt tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt
     9541 cttttctcat caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga
     9601 aagtagatgt aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc
     9661 aagcaaaacg atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta
     9721 gtcctcttta tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt
     9781 attttgaagt atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta
     9841 ctgttatcta ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt
     9901 ccactcctta tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg
     9961 ctacatgatt ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg
    10021 ttactcgata ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata
    10081 cttgctgaaa ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat
    10141 tgaaaggttt tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa
    10201 caagaaaaca aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa
    10261 tagaagacaa tgaaatttat atttcattgc attatccttg tgttattaga aaaagcatga
    10321 tatattaaat gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa
    10381 actccttttg cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg
    10441 aggaatttat ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc
    10501 tttatcactg gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac
    10561 ggcttcaaaa ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt
    10621 ggccctcaag caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc
    10681 gaaagaaaa
//
LOCUS       DAFCHQ010000087        10517 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000087 DAFCHQ010000000
VERSION     DAFCHQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.088"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..306)
                     /locus_tag="LNC71_002375"
     CDS             complement(133..306)
                     /locus_tag="LNC71_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6467397.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="LNC71_002376"
                     /pseudo
     CDS             754..3116
                     /locus_tag="LNC71_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="LNC71_002377"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="LNC71_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBK6467398.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="LNC71_002378"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="LNC71_002378"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBK6467399.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="LNC71_002379"
     CDS             5704..6489
                     /locus_tag="LNC71_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6467400.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="LNC71_002380"
     CDS             6502..7353
                     /locus_tag="LNC71_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBK6467401.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="LNC71_002381"
     CDS             complement(7477..7929)
                     /locus_tag="LNC71_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467402.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="LNC71_002382"
     CDS             complement(8394..8762)
                     /locus_tag="LNC71_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBK6467403.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="LNC71_002383"
     CDS             8912..9394
                     /locus_tag="LNC71_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBK6467404.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="LNC71_002384"
     CDS             9431..10441
                     /locus_tag="LNC71_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBK6467405.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DAFCHQ010000088        10502 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000088 DAFCHQ010000000
VERSION     DAFCHQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.089"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..656
                     /locus_tag="LNC71_002385"
     CDS             33..656
                     /locus_tag="LNC71_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467406.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="LNC71_002386"
     CDS             1106..2155
                     /locus_tag="LNC71_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6467407.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="LNC71_002387"
     CDS             2152..3033
                     /locus_tag="LNC71_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6467408.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="LNC71_002388"
     CDS             3036..3806
                     /locus_tag="LNC71_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6467409.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="LNC71_002389"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="LNC71_002389"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBK6467410.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="LNC71_002390"
     CDS             5117..6280
                     /locus_tag="LNC71_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6467411.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="LNC71_002391"
     CDS             6397..6807
                     /locus_tag="LNC71_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6467412.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="LNC71_002392"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="LNC71_002392"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBK6467413.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="LNC71_002393"
     CDS             9380..9664
                     /locus_tag="LNC71_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467414.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="LNC71_002394"
     CDS             9824..10060
                     /locus_tag="LNC71_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6467415.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="LNC71_002395"
     CDS             10057..10455
                     /locus_tag="LNC71_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBK6467416.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3662 a         1766 c         2226 g         2848 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 gg
//
LOCUS       DAFCHQ010000089        10424 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000089 DAFCHQ010000000
VERSION     DAFCHQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.090"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(141..383)
                     /gene="acpP"
                     /locus_tag="LNC71_002396"
     CDS             complement(141..383)
                     /gene="acpP"
                     /locus_tag="LNC71_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBK6467417.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(505..1506)
                     /gene="plsX"
                     /locus_tag="LNC71_002397"
     CDS             complement(505..1506)
                     /gene="plsX"
                     /locus_tag="LNC71_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBK6467418.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1555..3591)
                     /gene="recG"
                     /locus_tag="LNC71_002398"
     CDS             complement(1555..3591)
                     /gene="recG"
                     /locus_tag="LNC71_002398"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBK6467419.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3708..5390)
                     /locus_tag="LNC71_002399"
     CDS             complement(3708..5390)
                     /locus_tag="LNC71_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBK6467420.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5442..5804)
                     /locus_tag="LNC71_002400"
     CDS             complement(5442..5804)
                     /locus_tag="LNC71_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6467421.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            6074..6262
                     /locus_tag="LNC71_002401"
     CDS             6074..6262
                     /locus_tag="LNC71_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBK6467422.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6500..9169
                     /locus_tag="LNC71_002402"
     CDS             6500..9169
                     /locus_tag="LNC71_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBK6467423.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9624..10370)
                     /gene="truA"
                     /locus_tag="LNC71_002403"
     CDS             complement(9624..10370)
                     /gene="truA"
                     /locus_tag="LNC71_002403"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBK6467424.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2847 a         2245 c         1870 g         3462 t
ORIGIN      
        1 aaaaccgctt tactttgcaa cataatttct gtttatcgcc agaattatca gaaaaatgac
       61 ttttacgtgt caaaaagatt aaaaaaagaa ggataaaaag ctgcatgcag ctttttatcc
      121 ttctttgctt ttatttgaaa ttatttaaga tggctgctga tataatctac tgcgccaccg
      181 actgtttcaa tttgttcagc gtcttcatct gaaatttccg taccgaattc atcttcaagc
      241 tctaaaacga attccataat actaattgaa tcagctttta aatcatcttt gatgttcaat
      301 tcatccgtca ctttatcggt atctatctca aaatgatttg aaataatttt cgctactttg
      361 ttaaatactt cttcgcgagt caactttttc acctccaaga atcatacact tgtcaattac
      421 ctacaccatg gctaatttta ctggcatttt tcaggaatgt ccaccttttt ttcaatggaa
      481 cggatcgctt cagatatcca aatactattc ttcgcttttt tcaaattgtt cgactaattg
      541 tccaactaca tctgtctcaa gcattgtgtg gatttggcgg atcgtatagc gaacagcatc
      601 ggggcctgtt gctccatgag ttttaatgac tggtgctttc aagccaaaca aaacggcacc
      661 gccatgtttg gaataatcca tttctgcttt caaagaacgt aagccgtctt ttaaaagcat
      721 agcgcccatt tttcctttga tcccttcatt tagaatagca gattttaata gattcatcat
      781 attcattgct gttccttcaa tagatttcaa aacagcgttg cctgtaaatc catctgtgac
      841 cactacatca gccacaccat tgagtagctc acgagcttct acgtttccaa taaaattcaa
      901 gcttgtttca ttctgaagga gttcataggc ttttttcgtc acttcactac cttttgttgc
      961 ttctgttccg ttgtttaaga gtgcgacacg aggacgtccg atacctcgga ctttacgtgc
     1021 ataaaaagaa cctaaaatac cgtattgcaa caaatgttct ggtttgtttt ctgcgttagc
     1081 gccaagatcc agcatatcga aaccggcacc ttctttccct actactggca aggtagacat
     1141 caagccaggg cgttcgatat ttttgatacg tcctacgatg aacaaccctg ctgctagtag
     1201 cgcgcctgta ttccctgctg aaaatacagc atctgcttca ccgtttttca ctgcttgtgc
     1261 agcaaggacc attgatgcag tcttttttcg tcgaattgct tttactggct catcatcact
     1321 attgattttt tcgtctgtat ggataatcgt gatgtttttt tcatttgtta agtatttctt
     1381 gatttctgct tcttttccgt aaagtaagaa ttctacttct ggaaaatctt gctgcgctaa
     1441 agccacgcct tctacaattg cttgaggtgc gtgatctccg cccattgcat ctacggcaat
     1501 tttcattgtt tccactcctt tttccgtcat cagtataaag tatagcacag tctgctaatc
     1561 gaaataacga ttatcctgat tttctatttg gaggctttgc gctaaaggct gatactcagg
     1621 aagcatctgc caatccttta tttgccaaag ggctgcagcc tcttctctgg ctacctccaa
     1681 aacatttcca tccgtcacca agtcagcaat cgcaaattga ggcagaccag attgacggaa
     1741 gccaaatact tcaccaggac cacgtaattc cagatctttt tcgcttagta caaaaccatt
     1801 ggtggtctct gtcatgattt tcatccgttc cacacctagt tcatttttag gattagcaac
     1861 taagatacag taggaagcat cacttccgcg tccaacacgt ccacgaagtt ggtgcagctg
     1921 tgccaatcca aatcgatccg catccatgat cagcatgacg gtcgcgttcg gcacgtttac
     1981 ccctacttca attacagtag tcgaaactaa tatctgcatc tggttgtctt tgaaggcttc
     2041 catgattact tctttttcct gattcttcat tttaccatgt aacaagccaa cttcatattt
     2101 tggtgcaaat agttcacgaa gtttttcata gatttccact gcatttttta catctaatgc
     2161 ttctgattct tcaatcagcg gacagatgac atacatttga tgtcctcttg ccagttcttt
     2221 tttcgtccaa tccagtacac tgtccaattg aggtgttctt acccagcgtg tttcgatcgg
     2281 gatacgtcca gctggaagtt catcaatgat agatacatcc atctcaccat atgctgtaat
     2341 agctaatgtt ctaggaatcg gagtcgctgt cataaataag acatctggtc gccagccttt
     2401 ttcacgaagt actttccgct ggttcacgcc aaaacggtgc tgctcatccg taataactag
     2461 acctaaattc tgaaattcga ctccatcttg gatcaatgca tgtgtgccga tcacgacgtt
     2521 aatttcccct tgctctagtt tttcaaggag ctcacgtcgt tcttttgctt tcgttgaccc
     2581 cgtaatcaat gcgatggtta cttcattggg atcaaacagc tgttgcaagc tttccatatg
     2641 ttgttgtgcc aagatttctg taggcaccat caacgcacct tggaaacctg ctgtaacggt
     2701 agcgtataac gcaatcgcgg caaccactgt ttttccgcta cctacatctc cctgcaaaag
     2761 tcgctgcata tgctgcggac tgcgaagatc ccgacagatt tcattcgtta cacgtttttg
     2821 tgcatttgtc agttcaaaag gtagcttttg agtaaattgt ttcaattttt ggacatcata
     2881 aaggatctcc aaaccattcg tttctgcttt ttctgctttt ttcaatcctt gaagacgcat
     2941 ttgaaataaa aagaactctt caaaaacgac acgtcgtttg gcttgatggt tttcttcgtg
     3001 gtcttttgga aaatgcatac ttcttacagc agaagctcgg tcaagcagtc gatatttctc
     3061 cacaaggttg tttggcagat tttcttcaat caatgtgccg tattcttgaa accctttttt
     3121 gattagatca actaatgtcg tttgacgaat tgatttgttg acgtgatata tcggcgaaaa
     3181 gtcatccatt gtttgagaac ctaaaatctt catcccgttc aaggctttac gctttgcatc
     3241 ccatttaccg tatacggcga tttcctccga taaaatgact ttttctttta gataaggttg
     3301 attaaaaaaa gaaacgttga acacatcatg ttcttgcatc atacgaaatt gcaatcggct
     3361 ttttttataa ccaaatcgat tcatcacagg aggcgagact acgattcctt tcaaagtaac
     3421 cttttcttga tcctggatct cgtttagatt tctttcctga atatcttcgt agcgaaaagg
     3481 ataataagtc agtaagtctt caatcgtttg gatgcccaag ctggcaagac tagctgctct
     3541 tttttctccg actcctgcca gcatagtgat tggatcatct aaagaacgca taaatttttc
     3601 ctccctcctt taacaaaaaa aggacttggt cagcgatcat acctgatcac taaccgaagg
     3661 ccttttatga atgaatactg tgttagaaag atattctgac acagaaatta ttcagcagaa
     3721 aatagataag gatagacagg ctgatctcct tggtggatct cgatttccaa atcaggatgt
     3781 ttttctgtta gtttctcagc aaattcattt gcttgttcaa ctgttccttc atcacctgcc
     3841 aagatcgtta cgatctctgt gtcctcattg atcatttgtt ctagtgtact taacgctgtt
     3901 gctgtgatat ctgggttaga gatcaggatt tttccatcga tcatgcccaa aaagtctcct
     3961 tctttgatct ctacaccgtc aattgaggta tctctgatcg cgtgagtgac ttgaccgctc
     4021 acaacgctag acaacatttc tgtcatcgtt tctttgtttt cttctaaaga agcttgatca
     4081 ttgaatgaaa gcatagctgt caatccttgg gaaatcgttc ttgaaggaac aactgctact
     4141 gggatatctg ctacttctgc tgcttgatca gctgccatga agatgttctt attgtttggc
     4201 aagacaatca ctttatctgc gtttacttct tgaatcgctt taacaatgtc ttccgtactt
     4261 ggattcattg tttgtccacc actgataaca taattagcgc ctaaactttc aaacagctct
     4321 tggacgcctt taccagcagc gatcgctacg ataccatacg ggatacgtgg tatttctttt
     4381 ttcgcaaact cgttcacttc ttcatcgtgt tccaaaatcg tctcatgctg caagcgcatg
     4441 ttgtcgactt tgattttgac taatgaaccg aatttttgtc cataattcaa gacttctcct
     4501 gggttttctg tatgcacgtg gactttgatg atctcgtcat cattgaccac aagtaaagaa
     4561 tctcctatac catctaaata gttacggaat tcttcgtaat cgaatgtact atcgactgta
     4621 ggtccttctc cgatttttac catgatttct gtacagtaac caaaatggat atcttctgtc
     4681 gccatttgac cttggacact gcgatgatgt tctgcattga ccatatcatc catttctgct
     4741 ggagaaggtt catatgtttc atccgcttgg aattctccat tcaatgcact taaaaagccc
     4801 tcataaacaa acagcagtcc ttgccctcca ctgtctacga caccgacttc ttttaggact
     4861 ggaagcaagt ctggagtttt agctaaagca cgctttgctc cttttacaac agctgtcatt
     4921 acttcaacga catcatcagt actttttgct ttcttttctc cagctttcgc tgcttctcta
     4981 gctactgtca aaatcgttcc ttctactggt ttcattactg ctttatacgc agtttcaact
     5041 ccatgagtga aggctgcagc aagatcagaa gcatttaatg agacaacatc tggaatttgc
     5101 ttagaaaatc cacgaaacaa ttgagacaaa ataacgccgg aatttccgcg cgcacccatc
     5161 agcaatcctt ttgaaagcat tgctgctaat tctcctactt tttctgaggt tgaatctact
     5221 actgccttag ctccactagt catagataga ttcatatttg ttcccgtatc gccatctggc
     5281 acagggaata catttaatga attgacgtat tctgcattta cttgcagacg attcgcacct
     5341 gcctggacca tttcctggaa ctggcttgca cttatttctg ttacactcac ctagataatc
     5401 ctccttcaga tcgactgctg agttaccagc cagtctcctg cctagtctgg cagtacacga
     5461 acaccttgta caaagacatt aactgaattg gcagttacgc ctaataatgc ctctagattg
     5521 tatttcactt tttcttggac gttgcgggaa acttcagaga ttttcgtacc atagctgata
     5581 atcgtataaa catctacagc aataccatta tcttcttggc ggacaactac tcctcttgag
     5641 taattttctt tacgcaaaat ctcagtaata ttatctttaa tttgattttt gctggccatt
     5701 cctacaacac cgaacacatc tgtcgcggcg ccaccaacaa ctgttgcaat tacatcattg
     5761 gtaatttcaa tggttccagc tgacgtttga atttttacag ccatgaatga gcctccttaa
     5821 cgggtaaaaa actcccattt ttcttctatc atattttatc atagcgcaac ttgaaataaa
     5881 aggcagttac ccaaagtact caaaaacctt gcaaaatttc aggtataacc ttgtttaatt
     5941 tacaattatt cttctcaatt gtcaattgta tctatgaaaa tgtacttgca aattcccatt
     6001 ctatatgata tgttagtctg gtatgagctg aattattcta ttgctccaaa tcaaggcaaa
     6061 ggaggaaatt accatggcaa aagtttgtta ctttacaggt cgcaagacaa aaagtggaaa
     6121 caaccgttca cacgctatga actcaacaaa acgtactgtt aaacctaact tacaaaaagt
     6181 tcgcgttatg gtcgacggaa aacctaaaaa agtttgggtg tcaactcgtg ctttgaaatc
     6241 aggaaaagtt gaacgcgttt aattgaatga tacttggacc gttcagatag tgaacggttt
     6301 tttatttaat aaaaattttt aagggtggga cttttgattc ctaccctttt tttgcgttta
     6361 tttttgttat acgaacaaat tatgaagtta tttattctaa attttatttt tttgtgaaaa
     6421 ctagactctt tttaagcaga gaaaaacaag gatatgataa actgaataca acttcataga
     6481 aatttggtgg aaaataaata tgaaaaaaaa tttaaagtca ttctttagag aaaacggacg
     6541 ctacgcagca gctagtttcc tgattccgtt tttgatttta gcacttgttt atctaagtat
     6601 cggaatctat cctggaagca gcagaagtat tttggcaagt gatgcctttt cacagttttc
     6661 taattttcat gccagcttcc gaaatgttct tcttggaaag caaagtattt tttatacatg
     6721 gaatgcttct ctaggattga attatttatc tttaatttct tactatctag gcgggatctt
     6781 cacgccactg gtgttctttt ttccaaacca gatgatgcca gatgcactgt atgttctgac
     6841 tttgcttaag gtcggctgtg ccggattggc tttttggttt cttgcacgta cgttcaagat
     6901 tcctcgctgg gggcatgttg cacttagcgt cagttacgca ttgatctcat ttatcactgc
     6961 acattcggag atcatcatgt ggctggatgc atttttttat cttccattag tgattttggg
     7021 gattcatcgt gttatggatc ttaaacggcc gaaactttta tttatcagtt atttactctt
     7081 gtttttatcc agtttttata tgggttttat gattggcgta ttttctttct tatattttat
     7141 cgcccgtcta ttgacaaact ggtcagaata taaaaaaacg attgttcctt atggtatcac
     7201 ttctttactg gcaggcggcg cttcaatgat cattgtattg ccagcagtgc tggatcttcg
     7261 gtcaaatggc gaggcactga gtcagatcac tacgttgaaa acagaagcga cttcttatct
     7321 ggatctaatc atgaaaaata tgattggtgt ttatgacacg acaaaatacg gatcgattcc
     7381 gttcgtttac gcaggtctgc taccattagt cctctgtctg ttttattttg ccagtcggaa
     7441 agtaccgttg aaaaataaat ttttgtacgg cagtcttttc gccatactta tcgcaagttt
     7501 ttatcttgtt ccattgaatt tgttctggca tggtatgcat gcaccgaata tgtttttatt
     7561 ccgttatgcc tatctgttct ctttcttagt gctcctgctt gcaggaaaag gatgggaaaa
     7621 gatagaacaa gacgatcgtg ggctgttgtt aggaatcatt ttggtcgtag ctgctctctt
     7681 tgcggtggca tggggcatca aacctgaagg aagctacacc tatgtgacga tgacttcctt
     7741 tgtattgaca gtggttttct tagggattta tgcttttgct gtgggatttt atcagcataa
     7801 aaaactttca ttcaatcatc tgagtatatt gttattattg atcatgtctg ctgaagcact
     7861 ggtcaatacg aacagtatga tccatggcat tcttgatgat tggaattatg cttcccgcag
     7921 tttatatact gatccttatc ctgacttgaa agctcttgta aatgaaacaa aaaaagattc
     7981 cgactcgttt taccgcttgg aaaatctgga tccagtatct gcaaacgatt cgatcaacta
     8041 tggttacagt ggtatcagtt tgttttcttc tataagaaat cgtaattctt cctcttacct
     8101 ggataaatta ggtttccgtt caagagggac aaatttgaat atccgttata acaataatac
     8161 tttgctgatg gatgggttta caggaatcaa atataacatt tctaaaaacg aaagcacgtt
     8221 cagtaaatat ggcttccgcc aagctggtca aagcggcgac tatacactgt atcagaatgt
     8281 caatgcattg ccactagcat ttactgcacc gttgtccgtt aatgatgttg agcagccagt
     8341 gacagataat ttgaccagtc agacaaactt gatcaataca ttgtctggtc taaatgaaca
     8401 gtattttaca ttctatacac ctactttgaa gaatcagttg aatacgacga tcacaaagac
     8461 aacgactggc gtcacttact cagaaacagc agataaccag ccaaaagagc tcacttggga
     8521 agtccatgtg cctgcaaata cacaagctta cttaagtttg ttcccaacca attttgcgca
     8581 attagaaagt tctagtgcga cgatcactgt caatggtaca tcgcgaaaaa cgcaaatcaa
     8641 catttctggt caatactatg atcttggcta ttatcctcaa gagacaacag tgacatttac
     8701 tgcaagtttt tatgggacga aagaaataag tttcatggag cctaaagtcg ttggtctaga
     8761 tgttgtagcc tatcaagccg caatgaatca gatcaaagaa aaaggtgtcg aaatggaagc
     8821 cactggcaga aaagcttctg ggactgtaaa taccgctgaa gattccatgt tggtgacaac
     8881 tattccttat gacaaaggat ggaaagctaa aattgatggg aaaccagtca aggtcagcaa
     8941 tttcaaagac gcattcttga tgataaaagt tccagcaggt aaacatactg tcactttcag
     9001 ttacttgcct gaaggattta cgataggtgc aatccttttt gtcctatgta ttgcattgtt
     9061 tattctttat gtctggtacc ttgaccgcaa tcgccccttt tatggcacga ttgatccagc
     9121 aatagaagaa caaccacgaa acacacacag aagaagacgt agaaaataaa gaaacgatgg
     9181 aacctgttca gactgtccgc accaagggca atcaaaacaa cgttccatcg ttttttggtt
     9241 acagaaaaaa taggtaacaa aaagatgatt cacttatcaa cgcctcgtcc acaagtcacg
     9301 atattcatca aagatgagaa gctgtttttt gaaattgctc tttgtgactc agagttaaat
     9361 gacaggctca cgacctcttt cttaaacggt gttcaatcag ctgaccttcg gtattagctc
     9421 aacaaacggc aaaatatgag gagctatttt cccgtttgtt tctctaatac tcaggtcaaa
     9481 acgctgattt cacgacctct tccttaaacg gtgttccaga agtagcttct tcgaaaataa
     9541 gccgaaattt tgcaaaattt tgagaagtaa ttttcgtaaa taccttctta tttcttgaag
     9601 ctgaccactt ctatcataac ctcttattca tatttcactt catacaaata aagtccttca
     9661 gggtgtgctg taggtcctgc tgcattacga tcttttttcg caataatatc tgggatacta
     9721 tcttcaggca atcggccatt gccaattttc aaaagcgttc ctactaagat ccgtatcatc
     9781 ttatacaaga agccatttcc cctaaatgta aacaacaatt cgtcttttgt ttcatttacc
     9841 tccatcttag cctcgtagat cgtacgtatt ttatcctcta tcgagcttcc tgatgcacaa
     9901 aaagaggtga aatcgtgagt ccctaataaa tcaggaagcg cccgctgaat tttttccaaa
     9961 tcaagtgggt aaggaaaata acttgcataa tggcgtcgaa aaggactgcg tggtttccct
    10021 atatccactc gaaattgata cgtcttttct ttgaccaaat atcttgcgtg gaagtcttct
    10081 gaaacaattt ctacttgtct tacagcaata tcctctggtg actgggtatc tagcgcgaaa
    10141 cgcattcgtt ctaacggacg ctcctctgga taatcaaaat ggattacttg cccgatcgca
    10201 tgtactccgg catctgtcct tcccgaacca aagaccgtga tttcttttcc atttgccatt
    10261 ttttgtaatg ttttttctac ttcttcttga acagtacgcc catttggctg tttttggaat
    10321 ccattaaaat ttgttccgtc gtacgcaatg attgctttgt aacgtaccat gtttttctct
    10381 cctttgttcc taaaaggtta tctggaataa tgatttttct ataa
//
LOCUS       DAFCHQ010000090         9820 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000090 DAFCHQ010000000
VERSION     DAFCHQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.092"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..260
                     /locus_tag="LNC71_002404"
     CDS             51..260
                     /locus_tag="LNC71_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467425.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="LNC71_002405"
                     /pseudo
     CDS             467..667
                     /locus_tag="LNC71_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="LNC71_002406"
     CDS             complement(1379..1984)
                     /locus_tag="LNC71_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6467426.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="LNC71_002407"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="LNC71_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="LNC71_002408"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="LNC71_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="LNC71_002409"
     CDS             3019..3990
                     /locus_tag="LNC71_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6467427.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="LNC71_002410"
     CDS             4130..5443
                     /locus_tag="LNC71_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBK6467428.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="LNC71_002411"
     CDS             5486..6352
                     /locus_tag="LNC71_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBK6467429.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="LNC71_002412"
     CDS             6349..7197
                     /locus_tag="LNC71_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6467430.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="LNC71_002413"
     CDS             7211..8683
                     /locus_tag="LNC71_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBK6467431.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="LNC71_002414"
     CDS             8700..9572
                     /locus_tag="LNC71_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6467432.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         3081 a         1678 c         2047 g         3014 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta
     9721 tggaaatttc gatagccgta ggccacttgt attgtgcaat agttttttac aaaaaaaagg
     9781 gggtgcggat atttttttgg acctttttaa caaatatatc
//
LOCUS       DAFCHQ010000091         9811 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000091 DAFCHQ010000000
VERSION     DAFCHQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.093"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(34..2154)
                     /locus_tag="LNC71_002415"
     CDS             complement(34..2154)
                     /locus_tag="LNC71_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6467433.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="LNC71_002416"
     CDS             2384..3562
                     /locus_tag="LNC71_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBK6467434.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="LNC71_002417"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="LNC71_002417"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBK6467435.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="LNC71_002418"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="LNC71_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBK6467436.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="LNC71_002419"
     CDS             complement(4915..6168)
                     /locus_tag="LNC71_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6467437.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="LNC71_002420"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="LNC71_002420"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBK6467438.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="LNC71_002421"
     CDS             7746..9092
                     /locus_tag="LNC71_002421"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBK6467439.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="LNC71_002422"
     CDS             complement(9400..9786)
                     /locus_tag="LNC71_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6467440.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DAFCHQ010000092         9593 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000092 DAFCHQ010000000
VERSION     DAFCHQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.094"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..641)
                     /locus_tag="LNC71_002423"
     CDS             complement(<1..641)
                     /locus_tag="LNC71_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBK6467441.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="LNC71_002424"
     CDS             complement(643..1407)
                     /locus_tag="LNC71_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBK6467442.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="LNC71_002425"
     CDS             complement(1415..2092)
                     /locus_tag="LNC71_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBK6467443.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="LNC71_002426"
     CDS             complement(2129..3514)
                     /locus_tag="LNC71_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK6467444.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="LNC71_002427"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LNC71_002427"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="LNC71_002428"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="LNC71_002428"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBK6467445.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="LNC71_002429"
     CDS             4453..5310
                     /locus_tag="LNC71_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467446.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="LNC71_002430"
     CDS             complement(5391..5726)
                     /locus_tag="LNC71_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467447.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="LNC71_002431"
     CDS             complement(5793..7163)
                     /locus_tag="LNC71_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBK6467448.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="LNC71_002432"
     CDS             complement(7241..7777)
                     /locus_tag="LNC71_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBK6467449.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="LNC71_002433"
     CDS             8086..9567
                     /locus_tag="LNC71_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467450.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DAFCHQ010000093         9525 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000093 DAFCHQ010000000
VERSION     DAFCHQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.095"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="LNC71_002434"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="LNC71_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK6467451.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="LNC71_002435"
     CDS             1873..4539
                     /locus_tag="LNC71_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBK6467452.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="LNC71_002436"
     CDS             4903..5655
                     /locus_tag="LNC71_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6467453.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="LNC71_002437"
     CDS             complement(5677..6831)
                     /locus_tag="LNC71_002437"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBK6467454.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="LNC71_002438"
     CDS             7016..9457
                     /locus_tag="LNC71_002438"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBK6467455.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3077 a         1712 c         2033 g         2703 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ctgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg ctttt
//
LOCUS       DAFCHQ010000094         9249 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000094 DAFCHQ010000000
VERSION     DAFCHQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.097"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..648)
                     /locus_tag="LNC71_002439"
     CDS             complement(<1..648)
                     /locus_tag="LNC71_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBK6467456.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDP"
     gene            782..1069
                     /locus_tag="LNC71_002440"
     CDS             782..1069
                     /locus_tag="LNC71_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467457.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1485..3023
                     /locus_tag="LNC71_002441"
     CDS             1485..3023
                     /locus_tag="LNC71_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBK6467458.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3020..3985
                     /locus_tag="LNC71_002442"
     CDS             3020..3985
                     /locus_tag="LNC71_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467459.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3972..5294
                     /locus_tag="LNC71_002443"
     CDS             3972..5294
                     /locus_tag="LNC71_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6467460.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5357..5461
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5535..6278
                     /locus_tag="LNC71_002444"
     CDS             5535..6278
                     /locus_tag="LNC71_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6467461.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6293..7285
                     /locus_tag="LNC71_002445"
     CDS             6293..7285
                     /locus_tag="LNC71_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBK6467462.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7297..8034
                     /locus_tag="LNC71_002446"
     CDS             7297..8034
                     /locus_tag="LNC71_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467463.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8024..8746
                     /locus_tag="LNC71_002447"
     CDS             8024..8746
                     /locus_tag="LNC71_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBK6467464.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8830..>9249)
                     /locus_tag="LNC71_002448"
     CDS             complement(8830..>9249)
                     /locus_tag="LNC71_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBK6467465.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3024 a         1598 c         1957 g         2670 t
ORIGIN      
        1 aggatcaagt ggttcaaatg tttctgtagt ctgttttgct tccaattggg gcaaaacaga
       61 tccaatttcc agatacttca ctttattcaa tgtataagat agaatagaac agctaagtaa
      121 tgtatacaga aaatgtgtga gatccaaata aagcaaggag acaactaaaa taccaaaagt
      181 aatcaagaaa agacttttgc caatcttaat atgaaaatat ttttcaatgg cttgataaat
      241 gatatctgtt ccgcctgtgc ttccgccgaa tcgaaagacg agaccgcaac caatgccaga
      301 aagaatacct gtcatcaaac tattgatcag tagactatct acagcaaaat gtatatgaag
      361 gctctcccat aaatgtaaaa agaaaactag cgcaaatgtt ccgacgatcg tccggagaaa
      421 agcgtgtttt ccaaaaagaa acaatgagat aagcaataaa ggtaaattaa aaagcagtga
      481 agtgacagaa ggagcgaatc caaaaagatt atgcaaaagc aaagcaatac cagtaacccc
      541 accatctgcc agcttagatg gaatattgat tcctgtcaca gctaatccgt agattcctgc
      601 gcctaaaatg attccgcaca attctgtaaa taaatttaat tttttcattt ctagcctctt
      661 ttcttttgaa aattttcagc aacttcttta ttatactgac ttatactctt acttcaagag
      721 gaaaatgaaa aaaaattatt tttggctgtt tctttttatt tatcaaataa ggagtggaaa
      781 aatgcgagca gcagacttat tgacaattct tacaaaatca cccaaaaaaa cagagtatca
      841 aggagttttt ttggctcaag aacaaaaact ggtgcctgtg acaatggtcc agataaataa
      901 agcaaatcaa tttatctttt tatttgaaca cggcaaagca ccacttgcta tgaaagacat
      961 ctttcttttt ttgatgaaga accgtgagaa agctgtttat tatcagtcaa aagaagaact
     1021 cattcctctt tatggagtga aagagataga aaataaagtg gtgatataag taaaaataag
     1081 aaaacaagac gtttatgatg tcaaaacaac cattttttac atttttggca aaaaagtgtc
     1141 atgatacagt ataatattcg ttgaacaggc caataaaggt gttctccccc acgagatttc
     1201 tactttattg gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc
     1261 tgattccaag tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac
     1321 caaaaagttg atccgacttt gttgcttgga ttcttttttt gtcagcatat ttagaggtat
     1381 ttttttcttt tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag
     1441 gaaagaaatg tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac
     1501 aaacattaag tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt
     1561 ctctcatttc tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca
     1621 atcataaaaa aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt
     1681 cacttgcctt attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga
     1741 ttgcacttgt tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg
     1801 aaattgaccc gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct
     1861 tgctgaaagt agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct
     1921 tagttttaat catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga
     1981 catttatttt gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca
     2041 gttctgcctt gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac
     2101 ctcgatattc catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat
     2161 atcagtctgc cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac
     2221 ctgaaatcct gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg
     2281 aattaaaacc gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa
     2341 tcgatatcta tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag
     2401 caatgaaaag tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga
     2461 ataagcaatt cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg
     2521 cagtcaagag atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta
     2581 tctctgatca gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata
     2641 aaggaatcca agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt
     2701 tgcgttacca agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata
     2761 gccagctcaa ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt
     2821 atcaaggcgc accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag
     2881 cactgaataa attaagcgga aaaataacag aggatcaaat gattgatatg aattaccaag
     2941 tcaatgtcga aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat
     3001 tagtaaagga ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta
     3061 tgatggaaag tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt
     3121 tttggttggt ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc
     3181 taaaccatca gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca
     3241 gaaaatgcgg tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac
     3301 agtcaagcaa aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc
     3361 agaccgtgta gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg
     3421 tatgcctaga gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc
     3481 tgcaagccca gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag
     3541 aaactcatta caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt
     3601 cgttacgcac aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga
     3661 tggagaaatt atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta
     3721 cgttcggcat ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt
     3781 cgtcaatgga tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga
     3841 tacgcctatg aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga
     3901 aaagcaaaga agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga
     3961 ggaggaagga aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa
     4021 gtgctgctta tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat
     4081 ggggcgggac ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag
     4141 aggcacgtaa tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca
     4201 actgggaaga acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa
     4261 aaaaacaatt agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg
     4321 attcatctat tgaagtcaac gaagaagtct tccatgcgga ttacttagtt ttggcaaccg
     4381 ggcaaagacc tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact
     4441 ttttatcatt gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt
     4501 ttgagctagc tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc
     4561 aacggccatt aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag
     4621 catctggcat tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc
     4681 gctatcgttt agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg
     4741 ggagacagcc gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac
     4801 acggaattgc agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag
     4861 atattgtttc aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg
     4921 tagctaagcg gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga
     4981 tcgtctacgc cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt
     5041 cagatcaagt ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac
     5101 cagttgctaa ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga
     5161 ttagcgagca agcagacgaa ttgatcgatg atttgacact cgtcatcaat caaaaattga
     5221 ctaaaaaaga gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct
     5281 atttattgaa ataatcagca tgataactaa agcgcttgtt gctttttcct tcttgtgcta
     5341 cactttaaaa aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt
     5401 gaacttagac ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc
     5461 ttgaagcggg ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga
     5521 aaggaggtaa caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa
     5581 ttatttggca gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg
     5641 ctgctgtatt ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga
     5701 cattaaatga agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct
     5761 tggcaatgga ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac
     5821 aaatgttgcc aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc
     5881 ttccaaaagt gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg
     5941 atacattgag gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata
     6001 cagggaagat ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt
     6061 taaaagtggc tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc
     6121 aagcaggctt agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt
     6181 ttgctgcgat catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat
     6241 tgaaaaaagg gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa
     6301 attatggctt ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac
     6361 aagtagcaaa caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc
     6421 gaatactaat catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg
     6481 tttaaatgtc caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc
     6541 ggacaaagca caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg
     6601 acagaatata ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc
     6661 caccctatca gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg
     6721 ttggcaaagt ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga
     6781 cttttcaaaa ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc
     6841 ctcagatatc caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat
     6901 tgaactaaac tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt
     6961 aattattacg aatgatcaac tgatcaaaag tgatccagaa ttggtacagt cttttatgga
     7021 tgccacgaaa aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt
     7081 gcaaaaatat gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc
     7141 aaaaaattat acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa
     7201 ttatacagct tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact
     7261 attcactaat cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg
     7321 aatgtagtca ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat
     7381 acctttacat cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta
     7441 acaggagtcc agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt
     7501 ctccatacag catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc
     7561 tatcaaaacg ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta
     7621 agggattatc tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta
     7681 tctggcggga tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac
     7741 ttgcttcttg atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac
     7801 tggatgttga agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc
     7861 gaagaagcaa tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg
     7921 attgctgaga ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca
     7981 gagttgaagc aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca
     8041 ttgtcattta gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac
     8101 gatcatcaat tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa
     8161 gactcaagcc ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca
     8221 agttgagcct tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg
     8281 gacgaatgta ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc
     8341 cataaatgag aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta
     8401 tatccaagct taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca
     8461 agactatatt gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc
     8521 agtctggcaa ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact
     8581 acatgcagca gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct
     8641 tgaagaacag ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa
     8701 aaatacttat caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac
     8761 acaactagtg tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat
     8821 aaatgaatct tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc
     8881 ataacgtgaa gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc
     8941 atttaagtaa gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta
     9001 agaaccgctt gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt
     9061 agcagcagtt tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt
     9121 ttccgtttga accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac
     9181 ggctgcttga ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg
     9241 tcctgagta
//
LOCUS       DAFCHQ010000095         9020 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000095 DAFCHQ010000000
VERSION     DAFCHQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.098"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..738)
                     /locus_tag="LNC71_002449"
     CDS             complement(<1..738)
                     /locus_tag="LNC71_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBK6467466.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDY"
     gene            complement(748..2124)
                     /locus_tag="LNC71_002450"
     CDS             complement(748..2124)
                     /locus_tag="LNC71_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6467467.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(2244..2891)
                     /locus_tag="LNC71_002451"
     CDS             complement(2244..2891)
                     /locus_tag="LNC71_002451"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBK6467468.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2975..4402)
                     /gene="uxaC"
                     /locus_tag="LNC71_002452"
     CDS             complement(2975..4402)
                     /gene="uxaC"
                     /locus_tag="LNC71_002452"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBK6467469.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(4476..5555)
                     /gene="uxuA"
                     /locus_tag="LNC71_002453"
     CDS             complement(4476..5555)
                     /gene="uxuA"
                     /locus_tag="LNC71_002453"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBK6467470.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(5558..7189)
                     /locus_tag="LNC71_002454"
     CDS             complement(5558..7189)
                     /locus_tag="LNC71_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBK6467471.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7493..8164
                     /locus_tag="LNC71_002455"
     CDS             7493..8164
                     /locus_tag="LNC71_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6467472.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         2954 a         1757 c         1182 g         3127 t
ORIGIN      
        1 ataatctccc gataaacatt taaataaaac atcagttctt aagttttccc atgattcttt
       61 ttctttattg accaaaatag gataaaagaa aactttattt ttatttgcag cttctagatc
      121 accaggagaa tcaccaatca ttaatatctt atccaaatca caaccatttt caattacact
      181 tcgaataata tcttcttttt ttcctttatc ttgacaacac agttcatcaa ctaatgataa
      241 taattcatgt ctttcccatt cttcttgaac agcttcctta ttagctgagc ttacaataat
      301 tatcttccca tattttttca gagcttttat agtatctcta gcacttaaaa agggtttatc
      361 taatcctttt gcttgactaa tctgttgatt gacttcttcc gaccatttta aagcttttaa
      421 taaatcctct gactgagaat attctatttc cctcttcaaa gatggattag acaattcttt
      481 tgttagagat acccagtgct ttaatcgatt aatcgatgtt acatcatttt tttctaatgt
      541 tttaactaaa cctaaaaatc tgtttattcc acgagtctct gaatataaat taatattatt
      601 ccaatcctgt aggaattttt ctctatgatg aacatcaaaa atctctgcag caattggtcc
      661 gaaaaagaga ttgtgtttat aagtcatcgt atccataaca cagccatctg aatcaataca
      721 aaatatccaa ttattcatat ttctctccta tttatgtttt tgttttaaaa gctcgcaatt
      781 ttctaagact ttatctttag ataatggata aaggaaaaat aaaattaatc ctacaatcag
      841 aaaacaaatt gatggaatta atgttgaagc gttatataac ttttctaaaa catctcccgt
      901 ttgtacattt gctaaagagt catagcctat aatagaaaga gtccaacctc cgacgcctcc
      961 agctagagct tgacctattt ttctagcaaa agaatatacc gcatatacgg tcccatcttc
     1021 tctttgatca gactttactt cttgatcatc aataacatca gtaatatttg cccaaataac
     1081 agtattaaac acacccaaac ccatatagcc aaccgaacta agaattaaga atacatacat
     1141 attactagct tttatcaaaa ataagatcaa atatactatt gctgaaaaga acattcctac
     1201 tgatgccagt tctttttttc catatttttt acttaaaaac aaagttaaag gcgaaacaac
     1261 tattaaaacc aaaataggat tgataaaatt cattaaaact attccctgtg catcattata
     1321 gtaatcagga aatacataat tattcatact cataattaat aattgagcaa ggagtaagaa
     1381 aatagccgca ccaattatag aaagtaatgc cctattttta actaaccctt ttaaagattt
     1441 taacaatgaa actttgtatt gagattggtc aattcttact cgttcagtag ttagtttata
     1501 acatattaaa taacaaataa gtgcacatat cgaaaatatt cccgcaacaa ttgtaaaagt
     1561 tgatccacca cgaactattt gagcgcctga atcatctctt gtataaataa ataatggtgt
     1621 taatacacca atgaccaaac ttgctaaagt agctccaaca tttcggaatg tcgatagcat
     1681 tgttctttct tctgtaatag aagtaatagc tgatgccatt gatccataag gaatatttat
     1741 agatgtgtaa aaaatactcc cccataatat atacgtaaca tacatgtata ctaccttaac
     1801 gctcatagaa gctgaaatca tagaagtttg atacattaaa aaacttgcta aagcgactgg
     1861 accactcatt cttaaaatcc acggtttaaa cttaccctgc ttagttgctt tcgatctatc
     1921 aacaatacgc cccatagtta catcagtaac tgcatcgata aatcttgcta ctaaaaataa
     1981 tgttccaacg gcagctccgc ttatccccaa tacttttgta taaaagatca ttaaaaaaga
     2041 actagcaaaa atgaaagtaa aatcgtttcc aaaatctcca aacatatatc ctattttgtc
     2101 tcttatacca aattttcttt ccatacaatt ctctcccttc tgttgttaag cgttttcatt
     2161 ctgatgaaag tacgtactat actctgaagc gtaaagagtc agtatttatt ttttacattt
     2221 cagagtatta tttgtttttt ctcctaataa ttctttatct cataaaactt atctatatat
     2281 ttactagcta ctcgacttac ttcttcaaaa tcaccttttt caacagaagc caataaattc
     2341 ccacctacac caactgcaac aactcctact tcaaaccaat cttctaagtt ctctatactt
     2401 actccgccag taggcattat atttacgtgt ggtaatggag cttgataggc ttttacaatg
     2461 gatggaccaa aagcagaacc tgggaatagt ttaatgatat ctacgccagc agtaagtgcc
     2521 tcctttatct cagtaattgt catacaacct ggtaaataag gaatttgata taaattacaa
     2581 atctcagctg tttgtcgatc aaaagatgga ctaacgatat attctgcccc agccataatt
     2641 gcgatacgag cagtaaacgc atctaaaacc gtaccggctc cgattattac atctttttga
     2701 tcagcatatt ttttagcaat ttctttgatt atttcttcag cattaggaac tgtaaaagtt
     2761 agttcaatcc ctttaattcc acccgcaata attgaattta ttgctttaat ggcctcatcc
     2821 ttatttttcc cacgtacaac cgcaatgact ccagccgctt ctaacttact taaaatttct
     2881 acttttttca tatctatctc cttcctttat ggatacatac tttgtctagc tgtactaact
     2941 tacaaagaat aaatttaagc ttttatttca agtattattt tctacttccg atttcaaaaa
     3001 aattaaaata attatacgca ttacgataac aaatattctc cactatctga ctaagataca
     3061 tttcatcatc tggaattcgg cctgctttaa cccactcgct tagtaattga cataatactc
     3121 gacgaaaata ctcatggcga ggatagctta gaaaacttcg agaatcagtc aacatacctg
     3181 taaaattcga caacaaactt tgttgtgcca tcgtagtcaa ctgttgacgc attccttcag
     3241 ctgtatcatt gaaccaccaa gcacatccca attggatttt ttgaatcata cccccttgga
     3301 agctttgcat tgctgttgct agtggaagcc aatcagttgg atttaatgaa tataaaattg
     3361 tttttggtaa gcacccactc ttttcagcgg tatcgaacaa tgctacaaca ttttgtgtca
     3421 aactagcttg atcccccatc gaatcaccac cgacatctac tccaatttgt tcctttaata
     3481 tacttgaatt atttcttaaa gcatttaaat gcatttgcat cgtccaacca tatcttgtat
     3541 actcagacat caaatgtaat tgaatacctg taacaaattg tgcaatttct gataaatgga
     3601 aatccgtttt tcctgctaaa gcttttatta acagattgtc gagtattttt tcatctgcta
     3661 tttcataaaa ataagtattc aaaccgtgat ctgctaatcg acagccaatt tgatgaaaat
     3721 gatccactcg ttggctcaat gcttgcttca acccattaaa gtctttgatt ggtatagccg
     3781 ctgtttgact aagttttaaa atatagttcg aaaagcctga tgatttgata ctccagacct
     3841 tatctggacg gaaagtaggt aataccttga aagaattatc ctgtttagaa agctcagtat
     3901 gataccacaa gtcatctgta ggatcatccg ttgtacagat ggctttcaca tccatcatct
     3961 ccaacaattc tttcgggcga taggaatccg tttgaatttt ttcgttagca cgtcgccaaa
     4021 tttctggtgc attcttctca gaaattgtca aatcaatatc aaagtaacgt tgtaattcca
     4081 aatgagacca ttcaaaaata ggattacctg gcgccttctc cactgttttt acaaaagcta
     4141 aaaatttttc ataatcatca cctggaccac tgatcaactc ttcttctata ccattagctc
     4201 gcatcaatcg ccatttataa tgatctccag ctcctttatc atttaaccat acctgagtca
     4261 aattcttgta ttgcttattt tctagcaaat atgctgggtc taaatgacaa tgatagtcga
     4321 taattggttg gtgttttgca aatttatgaa aaagtctttt agctatttca ttctctaata
     4381 aaaagttctt atctaaaaac atatttaaac actcctttcc ttccgttagt ttttagaaga
     4441 gaaaattttt cctctaaata aactccctta tataattatt tactcgcccc cttttcaagc
     4501 gcttcccaaa taccatttaa ataaactgca cctagagctc ggtcatataa tccataacca
     4561 ggtctaccgg tttctcccca aatcatccga ccatgatctg gacgaatata gccattaaat
     4621 ccattatcgt acattgtttt taatatctta tacatatcta gagaaccata ttctgattta
     4681 tgcggcgctt cgtaaaaatc gagtccattg tcacttaaat atttgatatt acgaacatgt
     4741 gcaaaaggaa tccgatcccg ttgacaaaat tcagatagaa tttcatagac atcattatct
     4801 ggtcgttctg caatacttcc agtacataat gtaataccat tagaaggaga atccactaca
     4861 ttacacaacc aatctaaatc gtcacgattt ttaacaatac gaggtaatcc gaaaatggaa
     4921 tatggtggat catctgggtg aactgccatt ttaatcccaa cttcttcaca tgtcgggata
     4981 attgctttga gaaaatatgc aaagttctct cgtaatttct cttcatcaac ttctttatag
     5041 gcttctaata attgcttaat ttgtgataac cgatgaggtt cccaacccgg caattcaaaa
     5101 ttattagtag aattttctac ttctctaata atttcctccg gatcagtagg gatatttttt
     5161 gaaatatagg ctaacgtaga agacccatca tctaaacgat aatccaaatc cgattttacc
     5221 caatcaaata ctggcataaa attataacag ataacctcaa tcccaaattc tgccaaattg
     5281 cgaatcgtct gtttataatt ctcaatgtat tgatctcgtg ttggtaaacc aattttgata
     5341 tcatcatgaa cattaacact ctcaataact ttcaaggtaa gtccattggc ttcgacttca
     5401 tcttttaaag atttaattcg ttctctcggc caaacctctc cggctggtat atccattaat
     5461 gttccaacaa ttcctttcat tgctgggatc tgacgaattt cttgtagtga gattttttct
     5521 tcatttgccc cataccatct aaaagtcatt tccatagtta ctttcctcca acttctgtta
     5581 tatatttatt gattgtttta cgaactgctt gaggacctac tagcatttcg caaaagattt
     5641 tttcagtttt ctttcctacc ccaacttgat ataaatctaa accaaagatt tctttatttt
     5701 ctaaaatttc ctgtaccgca caatgaatct ttgaaggatc ttgttcacct aatttcaata
     5761 ctgataattt atcctgcaac tctaccaata acggatcagg acttggcaca aaattttccc
     5821 cttgatcatc gactgccagt aaataacgta accaagcagc aatagcgata ggaataaatg
     5881 ttagattttc tgcatttccg tctttttcga catacttttt aattgtttca ccatagcgaa
     5941 tcgctacttt ttgagaagta tctgttgcaa ttcgttgtgg tgtatctgga atatagggat
     6001 taggcaaacg tttattaatt acttcatcaa taaattgttt tggatcaata atgcctggat
     6061 tttcaactac tggtaaacct tctaaataac ctattccttt gattaaacca accaaatcat
     6121 catcctgcat ctctgctgaa attgattgat atcctagcag acaacctgtt actgccaaag
     6181 cagtatgaag tggattcaag cttgtcgtaa ccttcatgat gtctgcttta tcaaccgtct
     6241 ctctatctgt cataatcaca ccagcctttt ccaatttagg ccggccattt ggaaaactat
     6301 cttcaataac taaataatga actacctcag tattagtgaa agcagcaata ttggtatgtt
     6361 tttttgtgtg aatgatatct aaatcctcga gccccatcat caatagatct tcctttacat
     6421 tctctgaagg atttggcgta attcgatcaa tcatactcca aggaaaagtc actttttctt
     6481 tatcctttaa ataagcaata aatttttcag aaacatatcc ttctttttgc caagcttctg
     6541 caatcgtaaa aatactatct tgaaaacgtt gaccattctg tgagaaatta tcagtagaaa
     6601 ccatcgcaat cggcaactct ccagcattaa atctgctata taacaaggat gttacaatac
     6661 tcatcgtatg ttgaggattg ctaggtccag atttgatatc agctttgact accgaaaaga
     6721 attgcccttt agaatctttt aaactgtaac ctttttcagt aatagtaaac gttgtaaact
     6781 gtaatttagg atcttcaaaa tatttaacca ctctttgata gtccgctata ttcttcggat
     6841 tgcaatataa actatcagcc gttactgcca aaagtctttt tttgagttca ccatctccac
     6901 tcataacaat ttccaaaatg tcattgttat agtcatgata agctttttcc actacttctt
     6961 catcaaatgt ttcacacaca actactcctg tttctaattc attttgatct gctaagcatt
     7021 gagcaatttc tgcatgaaat cctcgataaa gatttcctcc accaaaatga agccatactg
     7081 gatgttgctg gccttcttgc ttaacttctt tgacatcaaa acacggcagt agaatctgca
     7141 tttcattgaa ttcttcgata gaattttgcc aatttttaat cgaaaccata attaacctcc
     7201 tcaaactgat atgttagttt tgatacattt tatattttaa aacccattgt aaccgttgtc
     7261 aatataaaaa gggaataaac ttgctttttt ctaccatatc agaatgattg tattttttat
     7321 tcagaaaaat aaaatactcc ttctttttgg actgatttta tcgaaactta ttttttcttt
     7381 ttttcatgta ttcttttatt tttataacta gtgtattact tcaaaatttg atattatatg
     7441 gcatgaacag aattaaaata tcagttaaaa taaaagttaa ggagcattgt gtatgacaat
     7501 taaaactagt ctccaaaatc aagcttacca atctattcgc cgacaaatta tctatgctga
     7561 cttagaacct ggaaaaaaag tatcagaaaa aaaattagaa gaaacacttt ctattggtcg
     7621 tacaccaata cgtgaagcac tgattcaact ccgacaacaa ggtcttgttg atacaattcc
     7681 tcaaagcgga aattatatct cattaattga tttagattcg gccaaaaatg ctcgatttgt
     7741 cagagaacat ttggaacgac aaattatgtt agagtgttgt gccaaaatga ataaccaaaa
     7801 aaaacggatt ttagaaacta taatttcaga acaagaaaaa gctgttagtc aaaaaaatgc
     7861 tcgtagtttt tttcataatg ataatttatt ccataaagct tgttttgaaa ttgcagagcg
     7921 agaagaagta tggcaatggt tagaaaataa caatacacac ttagaacgat ttcgatggct
     7981 acgagtaaaa acggaaggat taaagtggga aaaaattatg gaacaacatt atcaactatt
     8041 tgaggcatta acaaaacaag atccagaaga agctaacttt ctaacatcgt tgcatcttca
     8101 tttaatgctt aatgagcaaa atattgtttt agctactcat ccaaaatatt tcaaaaatat
     8161 ataactttta actcttttta aattaaatgg ttgttctgat cagaaaaata tcgcttgttt
     8221 tcttattatg taatatattt cataaactat tctgcataac aactattgtt tgtacttttt
     8281 acattggata actaattatg actgctgata acttttgata ttaaactagt atctccaata
     8341 caaataaaaa atagatatct tgttcatcaa aattagttcg tggatactta ccttcattta
     8401 catcattcag cggtttccca ttttctttta ttctattatt tcattttcta atagacttgg
     8461 cttcatttcg caatactttt tttcaatagg catcaaattg gtgttgttta gatatttagt
     8521 tgtagttttg tgacatgata agtatgctcc tttttctctt ttccactaat acaacgatca
     8581 aagggtgctt ttttcaaaaa actagaaaaa tcaaaataag attcgtattt tgattctata
     8641 tatcctgttt caactacatc ttatatttca caatagaata aatagccttt tgcatagctg
     8701 cttaacttat aagaaattgc aatttacttc ttcaaagtga aaacgaatat gagtaaaata
     8761 aaattattct tcttacagat aattaaaaat agcgcgtaac aaaaatcggc attcaatcac
     8821 ataattagga ttgatgccga ttacttatat tctacgtata gttttagtat tcttattttt
     8881 tatcaccagt aaccaattga gaacagccct aaagcagaat agtcttttat ctcaaaacgc
     8941 ttaatttcag gcttaggaat atgaataatc ttttgagtat attctttctc ttttaatctt
     9001 actgaagaaa taccactgcc
//
LOCUS       DAFCHQ010000096         8868 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000096 DAFCHQ010000000
VERSION     DAFCHQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.099"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            29..1369
                     /locus_tag="LNC71_002456"
     CDS             29..1369
                     /locus_tag="LNC71_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6467473.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1587..2030
                     /locus_tag="LNC71_002457"
     CDS             1587..2030
                     /locus_tag="LNC71_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBK6467474.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2534..3952)
                     /gene="nhaC"
                     /locus_tag="LNC71_002458"
     CDS             complement(2534..3952)
                     /gene="nhaC"
                     /locus_tag="LNC71_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBK6467475.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4355..5578)
                     /locus_tag="LNC71_002459"
     CDS             complement(4355..5578)
                     /locus_tag="LNC71_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBK6467476.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5609..6448)
                     /locus_tag="LNC71_002460"
     CDS             complement(5609..6448)
                     /locus_tag="LNC71_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBK6467477.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6465..7145)
                     /locus_tag="LNC71_002461"
     CDS             complement(6465..7145)
                     /locus_tag="LNC71_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6467478.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7147..8220)
                     /locus_tag="LNC71_002462"
     CDS             complement(7147..8220)
                     /locus_tag="LNC71_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBK6467479.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8276..8514)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8557..8790)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2541 a         1815 c         1615 g         2897 t
ORIGIN      
        1 ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag
       61 ctccagacat atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg
      121 aagtggcgaa gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg
      181 gctgatttta tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac
      241 gcatcacgga ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac
      301 tgattgggtc tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc
      361 ttcctttttg cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc
      421 tgttttaact acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg
      481 gctggctttt gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt
      541 cggtcagcaa atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg
      601 tgggtttact cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc
      661 ttacggcggt tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc
      721 gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt
      781 gtttttgctt ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt
      841 tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat
      901 tgtccttgct ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt
      961 cttccgtttg aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca
     1021 agtaccgcaa cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc
     1081 ttattttgtc ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt
     1141 attgatctgg atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt
     1201 atgggataaa ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat
     1261 cctgttcacg aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta
     1321 tttcagttat caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa
     1381 aattttattt tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag
     1441 tcttgttgca actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc
     1501 aaaaaccctc tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag
     1561 gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg
     1621 gtgtttcaac ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata
     1681 tcttcttcag tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca
     1741 tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag
     1801 ccgctagtct tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca
     1861 acgacacagc ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta
     1921 aacgagctga tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac
     1981 aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg
     2041 tccaaataaa aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta
     2101 cttctggaac gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     2161 cagccaaaca aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc
     2221 aagcttttga acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag
     2281 agagacaaat ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt
     2341 cagctgattg aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta
     2401 agaaaaattt tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa
     2461 actggctttt gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc
     2521 cctttttatg tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca
     2581 caaatttttt tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa
     2641 gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta
     2701 cgaaacttcc ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta
     2761 gcgttctacc taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat
     2821 taccaggcag aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg
     2881 tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca
     2941 tcacggttga gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa
     3001 tcaatgacac agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt
     3061 gttggtttcc agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg
     3121 aagtttgtgg gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg
     3181 ttaaaattgc cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg
     3241 accagattga aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa
     3301 gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg
     3361 ccgggacagt tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag
     3421 ctgctgctag atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg
     3481 aaacaatagc tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa
     3541 atgcaatccc aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg
     3601 cacacacaac aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga
     3661 ccattagagt tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa
     3721 taaaaataaa gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc
     3781 aatctgtcgg gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga
     3841 tcgtgatatt aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa
     3901 ccattaatac aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct
     3961 ctcctttttt agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca
     4021 attcatccgc gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat
     4081 gactccgcgg taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc
     4141 aaatgtatgg actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc
     4201 gactttctga acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta
     4261 ccttccataa gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta
     4321 tttttataac tttcacacac aagattattt tttactagaa agtagcgccc tcttgttttt
     4381 ttcttgctac tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca
     4441 acagatcaag agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt
     4501 tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga
     4561 ttgctaccgt aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac
     4621 cgagacttgt tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat
     4681 gctggaagaa taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga
     4741 acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca
     4801 ctttttcata tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag
     4861 cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat
     4921 tgacatcttt tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg
     4981 cttccaaagc atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa
     5041 atttgatgtc gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg
     5101 gatggacgca taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt
     5161 tagctgatcc gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac
     5221 cagtttcatc aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca
     5281 aattatgccc gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa
     5341 ttgtgggacc gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta
     5401 cttctttttc tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat
     5461 aaaactcata gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt
     5521 ctggtaaacg atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa
     5581 aaaattcctc ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca
     5641 cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt
     5701 ctactatttt ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca
     5761 cataaggacg tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa
     5821 atccagcatc tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac
     5881 gtgctgtttg ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat
     5941 cagaaacagt gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca
     6001 ataacagaag agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt
     6061 ctttgatatc agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag
     6121 tatttccgat cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt
     6181 gatgttggaa agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt
     6241 aatctgtaaa tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt
     6301 tgtcccatac atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt
     6361 cagttgtatc tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa
     6421 tgactacaaa acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt
     6481 ttaatcagtt gattactgat aaattgactt aaaaaaacta agattagaat caataatgta
     6541 gccacaatcg ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct
     6601 aatccgcctg cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc
     6661 aacgccgaga cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata
     6721 ccgattggac tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc
     6781 agtgcgttct cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa
     6841 gctgctttcg caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa
     6901 agcaagataa tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat
     6961 agcagacggt tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg
     7021 attccaatca tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc
     7081 gcttcgacaa attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca
     7141 gtcattttat cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa
     7201 aatatttatt tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt
     7261 ttctccagct aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt
     7321 gccatataaa atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc
     7381 gttcgtctga tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc
     7441 aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat
     7501 aaaatctttg gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc
     7561 aacgattctt ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat
     7621 ctcatgagtg atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc
     7681 aaggatctga agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac
     7741 cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag
     7801 ttgtttagga taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac
     7861 tttttgacga cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc
     7921 aaaaatagtt cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct
     7981 ttcttttcgc aattctttgg gagagagctg actcagattc gtctgattga caatgacttc
     8041 tcctgctgtt ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc
     8101 tgagtaaccg acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac
     8161 tgcctgtatc tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat
     8221 taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga
     8281 ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt
     8341 caagtacgat catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc
     8401 tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac
     8461 cgaagctctc tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca
     8521 aaaaaaaagt ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac
     8581 cacctttttt cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt
     8641 tcgttttaac ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg
     8701 ccattttcag cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc
     8761 caaacttact ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag
     8821 gaaaactgaa aagtcaagta cgaaattcaa attatttttt ctatattt
//
LOCUS       DAFCHQ010000097         8856 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000097 DAFCHQ010000000
VERSION     DAFCHQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.100"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            122..2719
                     /gene="adhE"
                     /locus_tag="LNC71_002463"
                     /gene_synonym="adhC"
     CDS             122..2719
                     /gene="adhE"
                     /locus_tag="LNC71_002463"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBK6467480.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2948..4054
                     /locus_tag="LNC71_002464"
     CDS             2948..4054
                     /locus_tag="LNC71_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBK6467481.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4071..5099
                     /locus_tag="LNC71_002465"
     CDS             4071..5099
                     /locus_tag="LNC71_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBK6467482.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5105..5584
                     /locus_tag="LNC71_002466"
     CDS             5105..5584
                     /locus_tag="LNC71_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBK6467483.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5600..6895
                     /locus_tag="LNC71_002467"
     CDS             5600..6895
                     /locus_tag="LNC71_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBK6467484.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6906..7730
                     /locus_tag="LNC71_002468"
     CDS             6906..7730
                     /locus_tag="LNC71_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBK6467485.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7895..8794
                     /locus_tag="LNC71_002469"
     CDS             7895..8794
                     /locus_tag="LNC71_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467486.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
BASE COUNT         2839 a         1537 c         1968 g         2512 t
ORIGIN      
        1 tttatattta aaaataactg tgaaaaaaat cacaaaaaag tgtttacata gaaaaataaa
       61 tattgtatga tatacatgtg aattatttaa caaataacca tgacccatta ggaggataat
      121 gatggctaaa gtaatagaaa aagaaaaaac gaagacaatt gacgtacaag caatgattga
      181 tgatttggca gaaaaagcaa atgcagcatt gaaagaaatg gaaagctttg atcaagaaaa
      241 agtagaccat atcgtgcatg aaatggcaat ggcagctttg gatcaacata tgccattagc
      301 gaagatggct gtagaggaaa caggccgcgg tgtttacgaa gataaagcaa tcaaaaatat
      361 gtatgcgtcc gaatatattt ggcataatat caaacatgat aaaacagttg gtgtgatcaa
      421 tgaagatgtc caaaaagggt tgattgaaat tgctgaacca gtcggtgtgg tttgcggagt
      481 aactccaaca acgaatccga cttccacaac gattttcaaa tcaatgatcg cgttgaaaac
      541 aagaaatcca atcgtttttg ctttccatcc aagtgcccaa aaatcttctg cagaagcagc
      601 acgagtggta cgtgatgctg ctatcgctgc cggagctcct gaaaattgta tccaatggat
      661 cgaacatcct tcgatcgaag cgacttctat gctaatgaac catccaggta tcgcgatcgt
      721 cttagcgaca ggtggagctg gaatggtcaa gtctgcttac tctacaggaa aaccagcttt
      781 aggtgtaggt ccaggtaacg taccagctta tattgaaaaa acagcaaaaa ttaaacgtgc
      841 agtgaacgat ttgatcgttt cgaaaacatt tgataatgga atgatttgtg cttctgaaca
      901 agctgtgatc gtggataaag aaatctatgc agcagtaaaa gcagaattcc aagcacacca
      961 agtttatatc gtaaaaccag atgaattgca aaaattagaa gatgcagtga tgaacgaagg
     1021 aaaatatgcg gtcaatccat caatcgttgg acattctgcg atggaaatcg caaaattggc
     1081 aggaatcagt gtaccaaaag gaacgaaaat gctgattgct gaattggaag gcgtaggccc
     1141 agattatccg ctatcaagag aaaaactttc tccagttctt gccatgatca aagctaacaa
     1201 cacggaccat gcgtttgatt tgtgtgaagg tatgttgaat cttggaggat taggacatac
     1261 agcagttatc cattcagaaa acgaagagtt gcatgtcaaa ttcggtcttc gaatgaaagc
     1321 ttgtcgtatt ttagtgaata caccatctgc agaaggcggc atcggtgata tctataacga
     1381 aatgattccg tcattgactt tgggatgcgg ttcttatggt aagaactctg tttctcgtaa
     1441 cgtctctgct gtaaacttaa tcaatgtcaa aactgtagcg aaacggagaa ataacatgca
     1501 atggttcaaa ttaccgccaa aaatcttctt tgaaaagaac tctctattgt acttagagaa
     1561 aatggaaaac gttgaacgtg tcatgatcgt ttgtgaccct ggtatggtcc aattcggtta
     1621 ctgcgataca gttcgtgaag tattggcacg acgtaaaaat gatgtgaaga tcgaagtctt
     1681 ctcagatgtt gaaccgaacc catcgacaaa taccgtatac gcaggaacga aactgatggc
     1741 agacttcaag ccagatacag taatcgctct tggaggcggt tctgcaatgg acgcagctaa
     1801 gggaatgtgg atgttctatg aacacccaga cacatctttc tttggtgcaa aacaaaaatt
     1861 cttggatatc cgtaaacgga catataagat cgacaaacca gaaaaaacac aattcgtatg
     1921 tatcccgaca acatcaggga ctggttctga agtaacgcca tttgcggtca tcactgacag
     1981 tgaaacacat gtgaaatatc cgttagcaga ttatgcattg acgccagatg tcgcaatcgt
     2041 cgatcctcaa ttcgtcatgt cagttccagc atctgtcaca gcagataccg ggatggatgt
     2101 gttaacacat gcaatcgaat cctatgtttc agtcatggct tcagattata cacgtggatt
     2161 gagcctacaa gcaatcaagt tagtctttga ctatttagaa aaatcagtaa aaacacctga
     2221 tatggaatca cgtgaaaaaa tgcacaacgc ttctacaatg gctggtatgg cgtttgctaa
     2281 cgcattccta ggtatttgtc actcagtcgc acataaaatc ggcggggaat acgggatccc
     2341 tcatggacgg acaaatgcga ttcttttacc gcatattatc cgctataatg caaaagatcc
     2401 acaaaaacat gcgatgttcc ctaaatatga ctatttccgt gcagatacag attacgcaga
     2461 tatcgcaaaa ttcttaggtc tgaaaggcga aacaacagag gaattagtcg aagctttagc
     2521 aacggctgtt tatgaattag gaaaatctgt cggcatcaac atgagtctaa aagctcaagg
     2581 agtcacacaa gaaacactag atacaaccgt agaccgtatg gctgaattag cttacgaaga
     2641 ccaatgtacg acagctaatc cgaaagaacc attgatctct gaattgaaac agatcatcat
     2701 tgatgcttac aacggataag caaaagaaag cgtagaaaaa agataagaaa atagatgaaa
     2761 aagaacttgg aaaacgttgt ttctccaagt tcttttttta atttccaagt aactgaaagt
     2821 tacttttgtt atttaacatt taggtcaatt ttagacagtt tttgttcaaa aagcgatatt
     2881 tatagtggac ggtattactg ctatattaag gatataaaat gaaagcgttt aaaaaggtgg
     2941 cgtaaagatg attattaaag aagagatttt tgtacctaaa gaattagtga aagaaaaaat
     3001 tgacctgctc gttgaaaacc tgacaacgat caaagacgat aatggtgaat ttttgttgga
     3061 ctttgatgga ctgaaagtag atgacaaaag ctggacagtg tggaattggc cacaaggtgt
     3121 cggtctatat ggtatttata aaaattacca gatgacaaaa aatcctcgcg cttatcaagt
     3181 ggtcaatgaa tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa acgtcaatac
     3241 aatggcaccg ctattgacta tggcttactt gtatgaagac acgaaagatt cacgctattt
     3301 gccttattta gaacaatggg cagaatgggt catgaatgac atgcctcgga caaatgaaga
     3361 cgggttacag catgccacat atggaccgga aaataagaat cagttgtggg acgatacatt
     3421 gatgatgaca gtgcttcctt tggcaaaaat cgggaaactg ttgaatcgcc cagaatattt
     3481 agaagaagct cgttaccagt ttatgatcca tatcaaatac ttgatggata aacgaacagg
     3541 attgtggtat cacggctgga cgtttgaagg aaatcataac tatgccgaag cattttgggc
     3601 tcgtggaaac tgctggttga cgattgctat tccagagatc attgaaatct tagaattatc
     3661 gaaagaagat gcattgcgca aattattgat cgaaacactt gaagcacaag tgcgtgcatt
     3721 aaaaacttat cagtctgaaa gcggtttatg gcatacgttg ttggatgatc cttcttctta
     3781 cgtagaatct tctgcaactg caggatttgc ttacggtatt ttgaaagcgg tccataaacg
     3841 ttatttacca caagaatata aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca
     3901 aatcgatgaa aaaggagaag ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt
     3961 agatttttat cggaatatcg gaatcacagc aatgccttat ggacagtcgt taacggtgct
     4021 ttgtttgaca gaattgctcg tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa
     4081 catcactgct gtttgcagca agttcgctta gcctaggtct gctaggtcta agcggctgcg
     4141 gaaataattc tagtgtggca actgtcgcag atgcagatgt aaatgagata gttaccttgc
     4201 gctttgctta tgcaagcaac agccagccag taattgatgc aatgaatgaa tttgggcgtt
     4261 tagtaaaaga aaaaacagat ggttccgtac agatccagta tttcccagat ggacaactgg
     4321 gtggagaacg tgaactgatc gaattgacac agtcaggagc agtagatttc acaaaagtaa
     4381 gtgcctcagc gttggaaagt ttctctaaag attattcgat tttttcagtt ccttatctat
     4441 tcaatgatga agaacatttc tttaaggtga tggataatga agaaataatg gagccaatct
     4501 accaatcgac caaaaattta ggatttaccg gattgaccta ttatgattct ggtcagcgca
     4561 gcttttatat ggtagacggt ccgatccata cgccggaaga tttgaaggga aagaagatcc
     4621 gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct tttaggcggt tcgcccgtac
     4681 ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa tttgatcaac ggatcagaaa
     4741 acaatgaatt tgtcttgtat acagctggac atggtggtgt ggctaaatac tattcctatg
     4801 atgagcatac acgtgtgcca gatatcatca tcatgaatga tgccatcaaa gaacagctaa
     4861 ctgatgagca ggaaaaagcc attgaagaag cagcgaaaga atcaacggtt tttgaaattg
     4921 aagcttttgc aaaagcaatt gaagaagaaa aagctttagc acaagaagaa catggagtcc
     4981 aattcaatga tgtagataat acaccgtttc gagaggcagt tgccccactt catgatatat
     5041 tcaaaaacga tcctacctac aaagaccttt atcaaaagat ccaagagcaa ggagcgtagc
     5101 tgtgatgaaa atgattttaa acaaggtatt agagatcctg ggagctgttt tgcttgttgg
     5161 gatggtcgct gttgttttat ggcaagtatt ttccagaacg gttttaggaa atccaaatac
     5221 ggtcacggaa gagcttgttc gattcggtct tgtctggttt gccatgttgt cttccgcgta
     5281 tgttgtggga caaaaagcac atttagccgt aacggtttta agtgaaaaat taactggcaa
     5341 aaaaagtaaa attttagaag ttgttgttca aagtttattt ctggtatttg ctgcgatgat
     5401 catggtgtat ggcggatgga atgctgtcac attgacaatg gggcagatct cgccttcttt
     5461 aagtattcct atgggatatg tttatctgtc agtacctgta tcgggggtac tcatcattat
     5521 ctatagcctg atcaatctaa tcgatacatt aagcaacaag ccagcaattc aagaaaaaat
     5581 ataaaaatgg aggaataaaa tggcaataga agccggatta gtgttatttt ttgtatttgt
     5641 tgtgttgctt gtattaggga tgccgattgc tataagtatt gcattctcat cgatgatgac
     5701 tttattgtta gttataccgt ttgatgtttc tgcttttagt tcagctcaaa aaatggtggg
     5761 aagtatcaat agtttttcgc tgatcgctat tccgtttttc gttctctcgg gcattatcat
     5821 gaataatgga gggatagcta agaaactagt ggattttgcg atgttgttcg tcgggcgcgt
     5881 gccgggggct ttggctcata cgaatgtgtt agggaatgct ttatttggtt ccatctctag
     5941 ctcggccatt gccgcttcta ccgcgattgg cggagtgttg atccctcagc aagtggaaga
     6001 aggatatgac cggaagtttg cgacagcggt caatatcgcg tcagctccga caggtatggt
     6061 cattcctcca agtactgctt ttatcatgta ttcacttgtt gctggtggtg cttcgatctc
     6121 tgctttgttt atgggaggtt atctcgtagg cgctttgtgg gcgctaggaa tcatgctagt
     6181 agcattcatc gttgctaaaa agaataaata ccctgttgtc gaaaaaggac agatggctca
     6241 tgctggaaaa ataataaaag aggcgattcc tagccttcta ttgattgtca tcattatcgg
     6301 tggtatcttg acggggatat ttacagcagt agaagcttca gcgatagctg tcatttactc
     6361 cctgttgatt gctatgtttt actataaaac ggtaaaacta aaagatatac ctaatatgtt
     6421 gtttcaatca gtagcaatgt cagggacgat catgttctta ttggctactt cttccatgat
     6481 gtctttcgcc atggcgttta ccggtattcc tcaggcaatt agttcgttga ttatgggcgt
     6541 aacggataat aaatttatta ttctgttatt agtcaatctt gtgttattac tggtgggtat
     6601 gtttatggat gtggcgcctg ctatcttgat ttttacacca atatttttac caatcgtcac
     6661 aagtgtaggg gttgatcctg tacattacgg gttgttttca atcatgaatc tgtgtgtagg
     6721 ctccatcacg ccgccagtag gtacaggact atatgtcggt gcaagtgtag gaggcgtaaa
     6781 agcagaggca atgctgaaac cgcttcttcc tttttatggg gttatcttag tcgtcttgtt
     6841 tttgattact tacttcccgc aaatcgtcat gtggctaccc aatatgctga gttaggagaa
     6901 tagttatgtt gaacattaca attagaggac atgatctttc cgaagtccaa acgatagaag
     6961 atcttgcaga aaaaacaaaa gaacaaggga ttcatacact gcagctcgcc ttaggtcttt
     7021 cgtttcctga aataccttct ggcgcaaatg agatcaattc tgggatggga aattatgtca
     7081 aacgagtttt agaaaagcaa gaagtgaccg taggaatttt aagttgctat atcaatatga
     7141 ttcatccaga tttaacgata cgacaagagt tgctgacaaa gttcgagcag tatcttcgct
     7201 atgcttcttc ctttggtgcg tcgatggttg cttcggaaac tggatgtgtt ttgccggaaa
     7261 tccaatatac ggaagaaaat ttcacagatg aagcatttgc tgaagctgtg tccgttatcc
     7321 gtcgcttggt aaaagctgga gaaaaatacc aaatgatggt ggggatagaa ccaggtttga
     7381 atcatccggt gtattcttta gcaagggtag agcagctgat ccaagcagta gattcggact
     7441 atctaggaat cattttagat ccgaccaatt tgatcacaag tagtgattat caagaacaag
     7501 tccaattggt agaagaagct tttgaacgtt ttggtgaaaa gatatgtgcg gttcatttga
     7561 aagattttcg tgtcgaacag gaaaaaatcg tgccggtaaa tttgggagac ggtgtgatcg
     7621 agtacacaaa aatcaaagaa atcatcatga agaatcgtcc gtacctgtat gtggttcttg
     7681 aagaaacaaa agatgatggg attcgttatg gaagaagtct tcttgaataa tgtaaagaag
     7741 acgttggtta actaatattt agaatggagg gggcggggct agaagatgcc acgtctcttc
     7801 ttttttataa gagtatataa aagcagctct ttttgattac aaaaaacgtg gtataataaa
     7861 attgtgcata agtgaacaaa aggaggtgcg gcacatgctc caaaagaaaa tcagcggtga
     7921 acaagtcaat acgatggttt ctgatcagta tgaaatctat catgtcaaag atgtcgtcag
     7981 ccaaaataaa acgatctatc atcatcatga tttctatgag atccatgcta cattaaaggg
     8041 agaagcgact ttttatttga acggacgaca atttacgatc aaagcaggaa gtgtgctgct
     8101 catccattcc aatgatttgc atcgtatcgt ccgccaatct tcagatgtat tcgagcgtgt
     8161 ctatatcttt gtcacccctt cttttctaga aagtcgttcc acgccatggt ctaatctatc
     8221 ggcttgtttt caaccaatgg gagagaagag gagtcgtgta ttgcaaatag aacaggaagt
     8281 attgaagcaa aaattatcat ttatcgacca tcctcctgat aaaaaaattt acggtgaaga
     8341 cataaaatat gaacaagctc tggtagaata cttgattttt ctcaatcaag ttgtacaaaa
     8401 agaagagagt gcgcaagaac agaatgctgt agttcctaat gagcgtgtgg aacaaatgtt
     8461 acaatatatc tcaaaaaatt tatctcatcc gctttcttta gaggaaatgg aaaagcagtt
     8521 ttttgtcagc aaataccata tcacacgaga atttaaaaag tacactggat ttacgtttca
     8581 tcagtatgta atgaagaaaa agctgatcta tgccaagcac ttgttgcgag aataccgcag
     8641 ctctagtgct gtatatagta aaagtggttt ttcttcttat ccgcattttc tgaaagcatt
     8701 caagagagag ttcggcataa cgcctaaaga atttttgaaa cgtgatacac agaaacagtt
     8761 ggttcattat gaacattttg aagatgaaca atgagaatga tcactaaaaa ataaccgcag
     8821 tagctgctgt tgtttacata gaagcagaaa ctgcgg
//
LOCUS       DAFCHQ010000098         8784 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000098 DAFCHQ010000000
VERSION     DAFCHQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.101"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            278..787
                     /locus_tag="LNC71_002470"
     CDS             278..787
                     /locus_tag="LNC71_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6467487.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="LNC71_002471"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="LNC71_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6467488.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="LNC71_002472"
     CDS             2322..3302
                     /locus_tag="LNC71_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6467489.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="LNC71_002473"
     CDS             3424..4842
                     /locus_tag="LNC71_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBK6467490.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="LNC71_002474"
     CDS             4913..6382
                     /locus_tag="LNC71_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6467491.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="LNC71_002475"
     CDS             6392..6859
                     /locus_tag="LNC71_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6467492.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="LNC71_002476"
     CDS             6895..7767
                     /locus_tag="LNC71_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBK6467493.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..>8784
                     /locus_tag="LNC71_002477"
     CDS             7849..>8784
                     /locus_tag="LNC71_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6467494.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEG"
BASE COUNT         3018 a         1315 c         1769 g         2682 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggt
//
LOCUS       DAFCHQ010000099         8776 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000099 DAFCHQ010000000
VERSION     DAFCHQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.102"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..1052)
                     /locus_tag="LNC71_002478"
     CDS             complement(21..1052)
                     /locus_tag="LNC71_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467495.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1059..1889)
                     /locus_tag="LNC71_002479"
     CDS             complement(1059..1889)
                     /locus_tag="LNC71_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6467496.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(1886..2701)
                     /locus_tag="LNC71_002480"
     CDS             complement(1886..2701)
                     /locus_tag="LNC71_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBK6467497.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(2698..3522)
                     /locus_tag="LNC71_002481"
     CDS             complement(2698..3522)
                     /locus_tag="LNC71_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBK6467498.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(3512..4612)
                     /locus_tag="LNC71_002482"
     CDS             complement(3512..4612)
                     /locus_tag="LNC71_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBK6467499.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4790..5575
                     /locus_tag="LNC71_002483"
     CDS             4790..5575
                     /locus_tag="LNC71_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBK6467500.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            5608..5991
                     /locus_tag="LNC71_002484"
     CDS             5608..5991
                     /locus_tag="LNC71_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBK6467501.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            6067..6198
                     /locus_tag="LNC71_002485"
     CDS             6067..6198
                     /locus_tag="LNC71_002485"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6467502.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            6167..6403
                     /locus_tag="LNC71_002486"
     CDS             6167..6403
                     /locus_tag="LNC71_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6467503.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(6481..7452)
                     /locus_tag="LNC71_002487"
     CDS             complement(6481..7452)
                     /locus_tag="LNC71_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBK6467504.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            8086..>8776
                     /locus_tag="LNC71_002488"
     CDS             8086..>8776
                     /locus_tag="LNC71_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HBK6467505.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPPFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT         2578 a         2018 c         1724 g         2456 t
ORIGIN      
        1 aaatgtttgc cgactataca ctagctttgt tgtgcaaaat gctgagtatc aatttccaca
       61 gggattgcta gaatatcttt cagctgaatg tataaggcat cgctaatttt ctcttcccga
      121 tcgttcaacc gatccactaa aatttctttg ccctccagcg tttgaaaaac ataccgttga
      181 atgttgccta aaataaatga ttccttccat agaaccggaa tcttaacccc ctgaggaacc
      241 ggttcaaatt gaatcagctc cggtcttaaa taataaatgc agcgattgcc cttagcggaa
      301 aactcgtggg tatactgatt cagctcatac cattcgaaac gattatagtt gccgataaat
      361 tccgcaacaa atcggctctg aggattctga tacaattcct tcgaggtgga aacctgagcc
      421 acccgcccag cttccatcac aaaaatccga tcactgattc gcatcgcctc ttcttggtca
      481 tgagtcacaa aaatcatcgt catccctagc gattgctgga tagcgcgcaa atcccgctgc
      541 agctgttttc taattcgggc atccaaagca cttaatggct catccagcag caggactttc
      601 ggttcagtga ccaatgagcg agccagtgcc acccgttgtc gttgaccgcc agaaagaaac
      661 tggacatttt tatctgcctg ctccgtcata tccaccaatg atagcatctc aaaaaccttt
      721 tcctgaatca cctcaggact cagcttcttt accttcaaac caaaggctac attttcaaaa
      781 acagtcatag ttggaaacaa tgcatactgt tgaaaaacaa agccgatatt gcgatccttc
      841 gtaggcttat cggcaatatt ctcattgtcc agcacaatct caccgctctt tggttgttcc
      901 aatcccgtga tacagcgcag caacgtagac tttccgcacc cgctgggacc tagtaaagta
      961 accagctcac ccttttcaat ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa
     1021 aacagattga gatcatgtat ttctaaaaac attttccgtc acaactcctt taagggcttc
     1081 tttttcgact gcttctcttc cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa
     1141 gtcagcataa aggtagttaa agcaattccc accataaaca gcaaatacaa aaccatcaca
     1201 gcgctagcca aatggccgtt ttcgttcatc cgccgcagca tatagatgcg taccgtctga
     1261 aatcgaccgc caatcaacag gttcgtcagc atgtactccc caaaaaccga agaaaaaatc
     1321 atcagagaaa ctagggtcac accgatacgg atgtttggaa acagcacctg gataataatc
     1381 gtagacatcg gggcgcccaa ggtttctgct gcatccacta attccttcag attaattaag
     1441 cgcatctggt tgttaagact aagatagaca atcggcaacg ccgtaataaa caatgcacct
     1501 accaacacta caaacattgg gataccagtt tcagcatacg ttcgaagcaa agccgttacc
     1561 aaaatcactc ctggtaaagc atacggcagc aggagcagct tttccagcca acgattcaaa
     1621 cgagggaaat acaaatgaat ccaaataatg gtgggaatca tcaacaacag aatcgtcacc
     1681 aagaccacac ctgctaataa aagtgagcga cccaccgcag caataaattc cctgtccgtc
     1741 agcagctgtt ggaaccattg caaggtaaaa tcactaggta aaagcgattt tgaccattta
     1801 gtggagctgg catacaaaat cgttccaata atcggcagca agtaatacag gacaaagcag
     1861 aacaaaatcg gttttattaa ctttttcacg tctgcaccac cttgcctttc ttttgaataa
     1921 tcaacattgc cgttaccagc aggacaccta aagcgactgc caacgcactg cctaaattag
     1981 gcttggcgaa cacatctccc gacactaaag ccgcaattcg aatcgttaaa agatttacat
     2041 tactgctggt cagggcatac gctgtttcat aggtccccat gccattagca aataagatga
     2101 caaaggtgct ggcaatcgat ggtcgcaaaa aaggcaaagc caccttgaac caagcatacg
     2161 ctgatgaagc gcccagcaat tgtgcggact ccagccattc ctttttcact tccttcatgc
     2221 tgggatacaa aaacaagacc cctaacggaa tttggaaata ggtatacgtg atggtcaatc
     2281 cccaccaaga atacaaattg aagtccgcta gaaatggcaa aatcattttc agcacaccta
     2341 cattccccaa caaaataata aaagagaatg ccagcggaat cccagcaaaa ttagctgcca
     2401 aattggaaac aaaggtgatt ttctcctgca atttagtcga tagattcatt aagcataaac
     2461 ataaaataaa cgtcccgatt aacccaatca cactggagct gacagcaatc agtacgctgt
     2521 taacaaaggc ctgtgtgtaa tatttgttgc tgaaaatatc actgtaattg gccaatgtcc
     2581 actggttagt taaatcactg tgcaagctgc acagaaccat aaaaagcagc ggcagcagta
     2641 aaaatgcaca gaagataaag gaggcaggaa ccagaaccaa gcgttttttc atctctttca
     2701 tcccctcatc aattcttgaa tgtcctgcat tttctctgac ggtgcaattc ctaataacca
     2761 gcaaaacaat ggagccgcca gcagctgtgg tacagtttgt tccttcagct tttggcgttt
     2821 atttgaaatc aaataaaaag gaacttcacg atgtgcagcc aagctgccgc catgcagtcc
     2881 gtgcgcatcc atcccgtgat ccgcagtcac cacgacttga taaccttttt gcagccattc
     2941 aggaaggtaa atagccaata atctgtccgc ctgattaacc gctgctgcat attcatgaga
     3001 atcggcggta aatttgtgac cgacatcatc aatattcatc gaatgtacga ccaaaaagtc
     3061 tggctgatac tgattcacta aataatgggc atccgcaaat aaatgcgaat cagggtaagt
     3121 gtcctcacta taaaagatgc catgctgaat cagtcgctcc tcctgatgct ggatgcgatc
     3181 aaccaaacga ttataggggg cttgattgta caactcgctg taccagtggt aagcggcggc
     3241 tcctgttttt ccgccagctt ccttaactaa ttcaaacagc gaccactctt tggatcgctg
     3301 agcatggtaa ttactataga tgccattttc ataggctgga acgccggtca tcagtacttc
     3361 gtataacgga cgtgaattgc tgggcagctc actttttacc ttcaataagc ttgcttgctg
     3421 gtgctcaact aaatgattca tgaacccaag ctgggcgacg gctgtgtcgt agcgacagcc
     3481 gtctagtata actacaatca gtttgtcaag tctactttgc ataggtcagt acctccgcct
     3541 gccaaagctc tggaagctct acagaggtct tgttccaagc ttcattgtct tcaactggca
     3601 ccgccgcttc ataagcatcc tccggcagta atttttcagc tacttcttta ggcagctcta
     3661 ccttgcgaat aggtcgcgca tagccctttg ccaaattgat ttgcccttca tcagataaaa
     3721 tatattcccg tgctagcttt gccgcattgg gatgaggggc atttttattg attaaggttg
     3781 tatacccgct aatgatggag ccgtctgttg gcacagttac ctcaaaacgc tcggagtcaa
     3841 tttggtcacg ataatttagt ccattgaagt cccaaacaac tgccacatcg atttcacctt
     3901 tttcaagatt tgctacgctg gcatcaacgg tggacaaacg gcctttttct gccatttttt
     3961 tgtagtaatt cagacctggc tgaatatcct tttcgtcacc tccgttggca atcgccgcag
     4021 ccaatacagc aaattgtgct tggttggcaa tcgtcacatc ccccacgcta acttgatagt
     4081 caccctcttg cagctccttc cagctagtcg gtgcttgctt cacattctct ttatcggtga
     4141 taaaggaaat ggttcccgta tagcttaaca accaatcccc atcatcatcc tttgcccaat
     4201 ccggaatttc ctcccaatag ttggtcttat aaggtaaggt taactctttg ctttcagcca
     4261 acggaccgaa gctgataccg acatcaccaa tatcggcggt tccatttttt ccttctgatt
     4321 caaacttagc caattcttct gcactggaca tatccgtatc cacgtgactt aagccgtatt
     4381 ctttttcaag gtcgttccaa gtgcctaccc aatttgccca agtatccggc attcccaagc
     4441 tggcaatctc tccttcttcc ttggcttttt ctataatcgt atctaaagag tcgtctgccg
     4501 cagccgattg cttcgttgct tcacctgagc cgcagcctgt cagcaggata cttactgttg
     4561 ctgcacaaaa taaaccgata gtcatgttac gaattttttc attcattttc attcattctt
     4621 ctcccctttc gataattcca ttctaacaaa gctttgtaat gagagaatca gtttcttgaa
     4681 aaggtttcat gaacaatgta aaggttgtat atggtttgta aatttcgacg aattcccgtt
     4741 aaccttctaa agcttttgcg gtatgatagt agaaaaaagg aggctcccta tgcagtatta
     4801 tgatcagcat ctccacacgt atttttcctt tgattcagag gaaaaatttg aaaactattt
     4861 agcctatcag ccggagtttt tcgtttctac ggatcacttt gatttaaaca atccatctac
     4921 cggaaaagat gacattccgg attatccagc ctatataaac aaactggact ctttaaaaga
     4981 aacctattcc accaaatttc tgcgaggtat cgagattggc gttgttccag gacaagaaga
     5041 gcggatcgtg gattacctcg ctcagcatcc ttatgattta aagctggtaa gtattcatca
     5101 aaatggccgc tttgattata tggacgatgt ggttttaacc aaagatccca ttcaaactac
     5161 caaggaatat tttgatcaga tgttgcaggt cttgacggat tttccagaag gagatatttt
     5221 gactcacttt gattatggat tacgccgctt caccttcaca gcggaagaat tcgcggagca
     5281 ttttgaggct cagctgctaa cgattttcaa aaaggttgtt gaacgagaat tggcgatgga
     5341 gttaaatgcc aagagcttct tgaaatacgg caacgaagac ctttaccgtt atgccgttcc
     5401 tttgtatcaa agtgtcggcg gaaagctctt taccttaggc tcggatgctc atgttgccga
     5461 agattatcag ctgggcttta aaaaaatgag tcagctgttg gcagactgtc aggtagaaaa
     5521 gttgctagtc attcaaggaa aagaacgccg cctgacgccg ctgccgattt tgtgattcct
     5581 atataataga agaaagtggt gaaaaaaatg agcaacctgt gggactacaa tcaagaggca
     5641 cccatccatt atttaattgc gcgacattgg gatgcgttga aaattgaggc ggtttgccga
     5701 tccttgttag cagccgttcc aaaacaacaa ctggaaaatt ttttggtggc tgacagcttg
     5761 cagcgggaaa aagttcaggc ttattttgca gcctttaaag atcagccttt ggaatatttg
     5821 catgcgcagt ttcatctttt ttatcaagta gccgccccag atgactacaa cgatttacga
     5881 ggacagctgc aattgacctt tcaagcggat gaaaccgcct ataccgtttt attgggaatg
     5941 gctcgccttg gtgatcaggc gaaggtcgag tggcggattt tcgatatttg atggcaggat
     6001 caaggcgttt cagttgcaaa ccttcctctc tttcaattag aaaaaccgca gcaggaagaa
     6061 gtttgtgtgc tggtcaccga tgctggtgaa attcgtctcc gcttatttcc tgaccaagct
     6121 cccctggctg tagccaactg gcgctcccta gctgagcaag gcgtttatga caacacacct
     6181 ttcggctcgt gtcattaagg aatttgttat tcaaggtggc gctttggatg gttctggtga
     6241 tgaagctgtt tccagctatg agggcttttt cgctgatgaa gtgcatcgcg gactttatca
     6301 ttttaatggc gccttggcct taggggacca cggcccccac accaacggca accagttttt
     6361 tattgtgcaa aacacaaagg cgcaggctga cctgctaatg taaagcaagc atacttggag
     6421 cttggaggac taccggagct ggatggtcgt tatactgttt ttggacaggt ataccaaggg
     6481 ttagaaatcg tagcagcgat cgcagctcag ccgacaaaca gtgaagatgt accgattgag
     6541 ccagtgttca ttcgacaaat taacttcgaa aaaacatcgt aaaaagacac tcactgactt
     6601 cagcaaaagc tctattcttg agctggctgc agctagtgag tgtctttcat ttatccataa
     6661 gataaagctt ggattcaata aataaataaa tgttttggtt aaagctgtga ccagcaaatg
     6721 aggaaccgtg cgagccagtt gatctgccaa tgcaaacatt tctttaggtg aaggcgctgg
     6781 cgtgtctgcc aacggatact ccagtcccaa tgcttggtaa ccgtgcatac catagcagtg
     6841 gtacggtagt aattctaacg gtacgaaccg atcaagatct gccacaaaac aagcaatcgt
     6901 cagcagctcg gtcaggttca tattgatacc tggaaccaca ggaactcgaa ttcgcacttc
     6961 gcagttagga tactcaatta aaaagcgacg aatattcttc aaaatttgtt ggttatctaa
     7021 gccggtccat tgtttgtgga gcttgctggt aaacattttt acatccacaa acaatgtatc
     7081 cacataggga gctacctttt ggatttcatt ataagcacca caaaagctgg tttccagtac
     7141 ggtattgatt ccaatgtatt tcgatttttg caaaatttct ttagcaaaat ccgcctgaac
     7201 cagtgcctcg cctccgctaa tggtcactcc gccatccgag tgaaagtaaa aaacagcatc
     7261 cttctcaatc tcatccatta cctcttcagc ggtcatggtc tggccaaatc ctttttcaat
     7321 ctgcttgctt tgtgattcgg gaatcgaaca ccaggcacag cgtaatgggc agccctttaa
     7381 ataaacaacg gtccgtaaac cagcaccatc atgtagagaa ccctgttcta ttcttaatac
     7441 tgttccttcc atatagattt ttgaattaca cactagttta aactaaaaca cacaaggaaa
     7501 aacttaccat ttaatcataa aattagctca tataaatata ctaggatgta aaatagactg
     7561 gtcaaaatct ggtcaaaaaa gaggactaat acaggtagtc ctcttttttg ttattttaat
     7621 attctaaatc attttttatt ggtttattcc cttattcgat gtagactcaa atcactatat
     7681 cactagatat caaacggcgt catacggaat caaaaacatc aaatttgacc tgttaaatgg
     7741 tcattgaata acattcaaaa tcaataaaac ggagtcagac acggagtcaa aaacgctgag
     7801 agtaaaaaat atatacagcc ttgtaggtaa agtaccacag ggctgtatat tttttatttc
     7861 agattacctt aaatgattcc atttactttt tatctcattt tttcctacta gttagtatca
     7921 aatactctcc tattttctca ataaactatt tttggataca tggtatctga aacagctaaa
     7981 accttgtcat tacagcaaaa aatcacgtta tccagactgt agacaaagtc cattttatgg
     8041 atgatgtcta cagtcttttc ttttataata gatttatgaa aacgaatgca aggagtgata
     8101 gctatgatgt ctaaaaacaa tacgaatggt agaaatcaat ttgcgatgct tacgattgat
     8161 gatctagttc ctcaagatca tctcgttcga aagattgatg ccgcgcttga ttttgaattt
     8221 atttatccaa tcgtcgaagc cacttactcc gatttaggtc gcccaagtat cgatccagtg
     8281 attctgatca aattggtgtt tattcagtat ttattcggta tccgttccat gcgacaaacc
     8341 attaaggaag tcgataccaa tgtcgcttac cgctggtttt taggctactc cttcgaggag
     8401 aaaatccccc ctttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
     8461 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
     8521 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
     8581 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
     8641 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
     8701 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
     8761 gaacgagaaa aacaat
//
LOCUS       DAFCHQ010000100         8673 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000100 DAFCHQ010000000
VERSION     DAFCHQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.103"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(271..2652)
                     /locus_tag="LNC71_002489"
     CDS             complement(271..2652)
                     /locus_tag="LNC71_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBK6467506.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2642..3289)
                     /gene="recU"
                     /locus_tag="LNC71_002490"
     CDS             complement(2642..3289)
                     /gene="recU"
                     /locus_tag="LNC71_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBK6467507.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3353..3892
                     /locus_tag="LNC71_002491"
     CDS             3353..3892
                     /locus_tag="LNC71_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBK6467508.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3982..4401
                     /gene="gpsB"
                     /locus_tag="LNC71_002492"
     CDS             3982..4401
                     /gene="gpsB"
                     /locus_tag="LNC71_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBK6467509.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4423..4790
                     /gene="rnpB"
                     /locus_tag="LNC71_002493"
     ncRNA           4423..4790
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LNC71_002493"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4999..6165
                     /locus_tag="LNC71_002494"
     CDS             4999..6165
                     /locus_tag="LNC71_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBK6467510.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6211..7707
                     /locus_tag="LNC71_002495"
     CDS             6211..7707
                     /locus_tag="LNC71_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBK6467511.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7788..8471
                     /locus_tag="LNC71_002496"
     CDS             7788..8471
                     /locus_tag="LNC71_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBK6467512.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2728 a         1572 c         1843 g         2530 t
ORIGIN      
        1 caatgggatt attaagaagt ggcactaaat aaaagactag tgtgtattta gccgatttaa
       61 ttataaacaa gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat
      121 ttttaagcta aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt
      181 aatacagctt ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa
      241 accacgcttg tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct
      301 ggatggtgta tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc
      361 aggaggagca gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt
      421 tgaagacgct ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga
      481 ttcgctttga ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg
      541 agtctggaca tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc
      601 tgtatattga tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc
      661 ccagtctgta tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga
      721 ggctacgtgt gacgattctg aagtgacagg cgtcattttt ttattgtaac ctgtccacac
      781 ggaaatcgaa taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga
      841 aataccaagt tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg
      901 atataaccca gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat
      961 gtctgtgatc atataagctg tttctggaga catggctgtt tttccatctg gctcataagt
     1021 ttcttctgtt ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc
     1081 tccattggcg aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt
     1141 cccatcttgt tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc
     1201 gatcccaaga ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt
     1261 gactgctgga acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata
     1321 ttgattatcc cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt
     1381 aatagttttt ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg
     1441 tttcatcgta gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac
     1501 gtcttctgcg atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat
     1561 aagggtggag gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac
     1621 gatatcatat agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc
     1681 tgtgtagaca ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt
     1741 atcgacgatc ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg
     1801 tacattaaca gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata
     1861 caaaacagta tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga
     1921 aggggcttga ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt
     1981 cttaccaaag tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac
     2041 tttatttacg tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc
     2101 catccacgct tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa
     2161 ggaaagtttg atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa
     2221 gttagagagg gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg
     2281 gcgatcttct accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat
     2341 tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt
     2401 ggaagatacg gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata
     2461 ccagaaaagt cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag
     2521 gatcttgata aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga
     2581 attcttcttc acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg
     2641 ttcatttgcc attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg
     2701 cgtccaaata aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga
     2761 tcaaagataa aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca
     2821 aatgaacact attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc
     2881 agaccccatt ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt
     2941 taaagggaaa agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc
     3001 gatagacgcc attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag
     3061 cagcacttct cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga
     3121 taaccgccaa cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc
     3181 gcatcccccg attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg
     3241 gagaggcatt cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca
     3301 ctcctaggcc acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa
     3361 gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa
     3421 tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga
     3481 agaagggctg gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca
     3541 aatagtagcg gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt
     3601 ttctgatttt ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca
     3661 attagcagat tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa
     3721 gaatcacact cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga
     3781 attccctgga aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc
     3841 ctacgaaatt cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt
     3901 gtgtttgatt tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa
     3961 atgaaaacta gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa
     4021 caagaattca aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac
     4081 aatatcatca aagattatga aacttacagc aaagaactcc ttgctttaca agaagaaaac
     4141 gatcgattga gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc
     4201 gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc
     4261 ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc
     4321 aaaccagcac aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat
     4381 gaaaaaacac gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg
     4441 tctgacttga tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt
     4501 cgtgcttagc gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta
     4561 aggcttcggc tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa
     4621 cccatccggt ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc
     4681 tttttgtctg gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg
     4741 ctttgttttt tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac
     4801 atcagggaat taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa
     4861 agctattttt caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc
     4921 ggttattcgg aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta
     4981 taaataagga gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca
     5041 agcgggctag aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt
     5101 gagaacggac gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg
     5161 ttacgtacag cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat
     5221 gaattgtttg aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct
     5281 tttcctgtag ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg
     5341 atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta
     5401 ccactgacgg aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg
     5461 atggtaactc tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa
     5521 ggtggagcac cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga
     5581 aaagaccgtc cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc
     5641 ttaataggcc ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat
     5701 tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat
     5761 gatattgaat tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa
     5821 gccaatgcag aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa
     5881 gacttcaaaa cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt
     5941 ctaggagaag aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca
     6001 ttaaaaacat ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga
     6061 caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag
     6121 tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt
     6181 cattcaattg gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta
     6241 aaagaaatca atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt
     6301 atgcctgaaa aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat
     6361 ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg
     6421 gaagaattgt cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg
     6481 gaacatcgcg taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac
     6541 ggacaatggc aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat
     6601 caaaatattg aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat
     6661 tatgatgttt tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta
     6721 tttgatcagc ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact
     6781 gaacctgaaa cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta
     6841 gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc
     6901 cacccattta tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa
     6961 acagatttca aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa
     7021 caagacatcg atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc
     7081 catgaatcac aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa
     7141 aagcaatatc ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac
     7201 aaattttatc gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt
     7261 ttgacttacc ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa
     7321 ggggtagatg ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga
     7381 atacgaccaa caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc
     7441 tttggttatt ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct
     7501 ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa
     7561 aactggatgt cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc
     7621 atggatgcaa cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg
     7681 tattttaatg tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct
     7741 aatctataga tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac
     7801 aagtgatcgc tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg
     7861 atcataccaa aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag
     7921 accgctttgt gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta
     7981 aaaatgagaa cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag
     8041 aggaacagat ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct
     8101 ctgaagcggc cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag
     8161 cattaggagc aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga
     8221 tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca
     8281 cgacagtgat caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg
     8341 attacggata tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat
     8401 tttttctgga atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata
     8461 acaaaaaatg atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt
     8521 ccgtaaaagt tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc
     8581 cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg
     8641 tgattcggaa ttggcaggag aacaccgttt att
//
LOCUS       DAFCHQ010000101         8555 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000101 DAFCHQ010000000
VERSION     DAFCHQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.104"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="LNC71_002497"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="LNC71_002497"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBK6467513.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="LNC71_002498"
     CDS             complement(1098..2201)
                     /locus_tag="LNC71_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK6467514.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="LNC71_002499"
     CDS             complement(2291..3247)
                     /locus_tag="LNC71_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBK6467515.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="LNC71_002500"
     CDS             3528..5462
                     /locus_tag="LNC71_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6467516.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="LNC71_002501"
     CDS             5919..6335
                     /locus_tag="LNC71_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBK6467517.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="LNC71_002502"
     CDS             6349..6915
                     /locus_tag="LNC71_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBK6467518.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="LNC71_002503"
     CDS             6938..7924
                     /locus_tag="LNC71_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBK6467519.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="LNC71_002504"
     CDS             complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="LNC71_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBK6467520.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2516 a         1650 c         1739 g         2650 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacgagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgct
//
LOCUS       DAFCHQ010000102         8540 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000102 DAFCHQ010000000
VERSION     DAFCHQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.105"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(111..608)
                     /locus_tag="LNC71_002505"
     CDS             complement(111..608)
                     /locus_tag="LNC71_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBK6467521.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(738..1448)
                     /gene="budA"
                     /locus_tag="LNC71_002506"
     CDS             complement(738..1448)
                     /gene="budA"
                     /locus_tag="LNC71_002506"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBK6467522.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1461..3125)
                     /gene="alsS"
                     /locus_tag="LNC71_002507"
     CDS             complement(1461..3125)
                     /gene="alsS"
                     /locus_tag="LNC71_002507"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBK6467523.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3330..3992)
                     /locus_tag="LNC71_002508"
     CDS             complement(3330..3992)
                     /locus_tag="LNC71_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBK6467524.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4002..4817)
                     /locus_tag="LNC71_002509"
     CDS             complement(4002..4817)
                     /locus_tag="LNC71_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBK6467525.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5079..5522)
                     /locus_tag="LNC71_002510"
     CDS             complement(5079..5522)
                     /locus_tag="LNC71_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBK6467526.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5656..5994)
                     /locus_tag="LNC71_002511"
     CDS             complement(5656..5994)
                     /locus_tag="LNC71_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6467527.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5982..6359)
                     /locus_tag="LNC71_002512"
     CDS             complement(5982..6359)
                     /locus_tag="LNC71_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBK6467528.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6583..7776
                     /locus_tag="LNC71_002513"
     CDS             6583..7776
                     /locus_tag="LNC71_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6467529.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7942..8364)
                     /locus_tag="LNC71_002514"
     CDS             complement(7942..8364)
                     /locus_tag="LNC71_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467530.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2516 a         1720 c         1428 g         2876 t
ORIGIN      
        1 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgccc actgtctttc
       61 cggctctttt tattcttgat ttctcttctt ttttgtttca atagacgatt ttagttatta
      121 ataaatgaaa aagcatttat attaaaattt gctaataaaa gatctacata tttttctaga
      181 aatgcttgat cgatttcatc ataccctttc gttactccgc aatcgatatc taaaacaccg
      241 attaaattac cttcttttac cattggcact acgatttctg attgtgcggc tgaatcgcag
      301 gaaatgtaat ttgcatgggt tttgacatca tcaacgatca atgtttcttg tttttcagct
      361 gattctccac aaactccttt tcccatcttg atacgtgtgc aagaaactcg cccttggaac
      421 ggtcctaaaa tcaattcatt accatcgaat aaataatatc ctgcaaagac agtctcaggc
      481 aaagtctcat tcaacaatgc agatgaattt gctaaatttg ctaaccagtt atcttctgct
      541 tctaataatc cttgttgctg taataataaa atttcgtatg catctttttt ctgtttttct
      601 gtccacatga taaaaaccgc tcctttttga tctatggaga agattatagc tcagtttttt
      661 tgtaaacgct aggtttcctc ctcaaaaaaa cgagatacgt caggttaatc accttgaacg
      721 catctcgctg tctttttcta ttctgcttgt tggatttctg ctgctaaatc agcataatca
      781 atcgtttcgt gaacaaaagt cggatcatca actggaaaat gctgtacaag ggacgagatg
      841 cggctaatct caacttttcc ttcagccact tcaaaatcca ttacatgtcc gccaaatgta
      901 tgtgtatcat cgataaaatg caagtgaaat cccgctgcag caactccttg gaataattca
      961 ggcgtaaaga acccgactaa tgttccttgg acattgcttt gttggaattc tggttgagac
     1021 tctgaaattt ttgctagtct tctgtaaggt ttttcttgct tcggcatgat acggatgtgc
     1081 atatttttga ataatccagt tatcttcact gccaaaaata ggttttcccc ggcttctctt
     1141 actaatatgt cttttttcaa ttgttctcca tctgcagttt ttgagacaga aaaactagta
     1201 tctggcgtaa aatctgtaat tgctgcatag ggggtaagtt cacttccatc taattgggca
     1261 actgagccat ctgatcttcc ttgataagct acgccatcta aaaagatgac ttcaccatca
     1321 agcccatgca atgttcccaa tcccagagtt cctttttcta atatatgagc aatcgtttct
     1381 gttccatcca tcaagccagc catcaaagct cctaatgtcc cgtgctggta taagattttt
     1441 tcagtcatgt cgacacttcc ttaatagaat tgatctggta agattgtttc tcccaatttc
     1501 tcattatcac ggtaatcaat cggaatatca ataattacgg gtccttctgt cgcaaatcct
     1561 tcttctaaag ctttttctaa ttcctctaca cttgtagctc ggattccttt agcgccaaaa
     1621 gcttctgcat atttcacaaa atcaactgga ccaaagtcta cacctgacgc ccgctgatat
     1681 ttcatttttt cttggaactc taccatattg tagtgtccat cattccaaat caaatgaacg
     1741 atattcaatt ttttacgtac cgccgtttct aaatcctggg cagagaataa aaatccccca
     1801 tcgccggaaa ctgatacgat ttgtgtattt ggacgcacca atgcagcaga aatcgcccat
     1861 ggcaatgcaa caccaagcgt ttgcatacca ttactgaata acaaatgacg aggttcataa
     1921 cttctaaagt ggcgtgccat ccaaatataa tgactaccta catctacggt aacagtcata
     1981 tcatctgtta cttttgactg aagcgtgtta ataacttcta aaggatgcaa gatacctgct
     2041 tctccttttg attgaacaat gtcccgctcc gttaattctg cttgaagaga tgctaaatat
     2101 tcttttgcat cttctgacac ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg
     2161 gctgagatat caccgatcaa ttctctttca ggctgcataa aaggatcgat ttcagctggc
     2221 gcttcatcga tcacaatgat ccgcgcatct ttttccgcat tccagtttct tgcttcatat
     2281 tcgattggat catagccaat agcaataact agatcacttc gtttcaaaag catatctcct
     2341 ggttggtttc taaataaacc cacacgacca aagaaatgcg cttctaattc acgtgagatc
     2401 actccagctg cttggaatgt ctctacaaca ggcaatgctg tctttccaac taattgcctg
     2461 atggctaacg tttctttttc acttgatcct cgcataccta ctaatagtac cggtagtttt
     2521 gcttctctaa tgcgttcagc taggtaacgg atatcatctg cagaagcaga acctaattta
     2581 ggatcgctca atggcttgat gacattccct tggacaggtg catccactac atcttgcggg
     2641 atactgataa agctcgcgcc tttttttgaa ctcttagcca tacgataagc attggcaatc
     2701 acttctgaaa ttgtttctgg atcttggatt tctgcactgt attttgtgat tggctggaac
     2761 agtgctgcat tatccatgct ttgatgggtc aacttcaata gatcgcttcg ttttacttgt
     2821 ccggcaattg ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag
     2881 ttactagcac caggaccact tgttgcgata actactcctg gctctcctgt aatacgtccg
     2941 attgcttgtg ccataaatgc agcattttgt tcgtgacgag tcacgattaa ttcaggacct
     3001 tgatcctcta attcattaaa tacaccatcg atttttgcac ctggtatacc gaatacataa
     3061 tcaacatgat gattaatcaa actttctact acagatttcg aaccttgttt cattttttcg
     3121 tccattgaac atcgtccttt ttatgcttta tttttaatta ttagttagaa ataatcacaa
     3181 ataattgata cagtttctaa acctgtttca ttcaaaagta tagcacgttt tatttcattt
     3241 gtttagccat atgtttcaaa aaatgaaaaa gaatgacagt ttttacataa aaactgtcat
     3301 tcttaattga aagtttcaat ttataaagtc tacagatcca tagttctcat caatttacga
     3361 ttgttgaagt aaatcaaacc aaatacgata gaaggaccta gtagaataca ccattggggc
     3421 tttacgaaaa agaagagaaa caaacttgtc aatagtaaaa aatctagtaa actcacatac
     3481 cattttttca acgtatagta gaacgctatc cgtagaactt gatttggttt cgacgctgga
     3541 tttctcacct gaaaatacca gtagttgatg ctaaaggaaa ctgataaaca agcaagtaaa
     3601 acaaacaacg gaatcaacca ttttccataa gaaaaacgta taaaaaagaa acaatcaaaa
     3661 atcattatca cactaactat ccaagcaaat agccaataaa aaaatccctt tagccaaaat
     3721 tggcgaaacg tctttatgaa caaacgtact ggtgaaaaac ttttttcttt tgctaattct
     3781 tctgtcatac ctatcatcac gatgcaacct ggaccaataa aaagtaaaga aaaaataaag
     3841 gaaaagacat tccttccatc tatcgacaaa aggaacgcag ctgctatata caagatattg
     3901 acacaccaaa aacaaaggct acttgccaat gattgataca accaataaaa gaattccata
     3961 taaacatttt tgtcaaaacg ctgctctttc ataatcacct cctattcctt cattcctgat
     4021 gaagcgatcc cttctacaaa atatttttgc atcgctaaaa atataaaagc aacaggaaga
     4081 acagaaacaa ctgaggcagc catgattaat gcgtactctg catcaaatag cccaataaac
     4141 attttcaacc ctaactggat tgttttattt tccgtagaag acaaataaat gaatggcccc
     4201 atataatcgt tccatgtatt aacgaatgtg atgatcgtca aactggcaat cgccggtttt
     4261 gtaagaggta aaatgatctt gcgatagatt ccccactctg ataagccatc gatccgcgca
     4321 ctctcacata aagaatctgg gatactactg tagtattgct tcaacaagaa cacaccaaaa
     4381 gcattgaacg cttgtaatag caccaacgac cacaacgtat atgttaaacc cacttttctc
     4441 atcataatga actgcgggat catataagac tgccaaggga cagcaatcgt gccgatatat
     4501 gctaaaaata aaatatcgcg ccctttaaaa tttagtttag aaaacccgta agcagcaaaa
     4561 ctagaagtaa acaactgaat caacgtgatc acaacgctta aaaaagctgt attcttaaaa
     4621 aatgtcacta aaggaatacg agaccaaata accagataat tctctaaatg ccaagtttta
     4681 gggatccatt gaatcggaat cgtgaacact tcattatttg ctttgaatga agcagaaacc
     4741 atccataaaa aaggaatgat cgtcaataaa gctaatacaa tcagcagaat catacccaac
     4801 cattgaataa agggcatgtt ttttgaacga atttttgctt tttttctttc atttcgaaca
     4861 cctccaaagt tataatcttc atttctttgt aatgattaca atccagaaca acggttatta
     4921 tagctgattc atttccgttc ttcttatgtt tgtaagcgct tatttaacaa gttatgtaaa
     4981 tctctttatt ttttttgatt taaaaatgaa aaaatacaaa agcaagactg tcatacgtct
     5041 cgcttttgta ttgaaatttc ttcgattaga gatacttatt actgttcaaa agcttctttg
     5101 aaacggtgaa tcaattcatt gactacaggt gataaaatcc gattcttttt ccagactagc
     5161 atgcagttcg tttcaatcat tgggtcaagc ggaacgaatg tcactcgctt tgaaagatga
     5221 cttacagcac cttctatgac taaagcagca cctacctgat tctcaattag cgcaaataca
     5281 ttgaaaatca aattgtacgt tcctgtgatc agcaactgct ccttttttat gcctaaccag
     5341 tcagcaaaca ttcgctgtat ttctggacgc tttgagatca aaagaggtac attcgcaaag
     5401 tcttcttttg ttatcacgtc tttttttgct aaaacagaat tttctaaaat gagcaatccc
     5461 catttttcct ttcttggtaa caaaatcgtt tcgtatttct ctgtatctac tggatctagc
     5521 aaaatcgcta aatctaacag gcttttttct agtcactccg tgatatcatc acttgttccg
     5581 ctgtggatat gaaattgaac gctgggataa tcacggatca acttttctag taatagtgcc
     5641 aatgtattcg aattatcagc ttccacacag ccgatcgtaa atcggccgct gaataattgt
     5701 ttttttctgt tctcgaattc ttgagtggtt tgattcgtta aatctaaaat ttcttctgct
     5761 cggctttttt agaaaataac ctgcttctgt caagatcatt ttgcggtttt cgcgtacaaa
     5821 cagttctgtt cctagttcct cttctaactc cttcagttgt cggcttaagg ttggttgtgt
     5881 aagatgtaga acttctgcgg cttgagagat tgttccttct tctgcaattg tccaaaaata
     5941 ttttagtaag cgtaactcca tagcgatgct ccttgatttt tttacttgat acattccatt
     6001 ttatttctag ataacacacg atcatatttc ttgatttttc cttgtaaacg ttccaaacac
     6061 tcttgcattt cagcaatttc tttcgtcaaa agttcacttt cttcaatcaa aagcttacgc
     6121 cgctcaaata cagttgcttc tccttcgcta gataggcgaa tatattcaat caaagactcg
     6181 atcgataatc cggcatttcg catacagatc gtaaagtcga cccattttag gtcttcttct
     6241 gtataatcac ggattccgcc ttttgtccga ttaactggtg gaatcaaccc gattcgttca
     6301 taatagcgca atgtatcggc cgaaatgccc ttttcttcac tgacttgttt gatattcatc
     6361 tgatcactct ttctcttaat ttcttgccac tttacttatg tattttattt attctacaac
     6421 tttaagtgta ctccaagtca atactcattt ctattaaaac taagctggat tatctatatc
     6481 ggttgtttgt gtattatttc aaataaaaaa gtcttgtttt tttattcttt tttggatata
     6541 cttttaacaa tttagggaaa ttttaggaaa aggggtataa aaatgacaac gtatcagcga
     6601 aaggtattat tatcgacatc ggcgggaatt gctttagaga atatggatat tatgttttta
     6661 gctttttcat tatcttcaat gattacttct tttcatatta gtggtacgca agcgggtttc
     6721 atttcaacga tcactaattt ggggatgctc attggaggta ttttttttgg catcatagct
     6781 gataaatatg gacgtgtgaa agtattttct caaacggtcc ttctgttttc gattgcttct
     6841 ttattgatgt attttgcttc aaacatctac ttggtttatc tctttcgctt tatcgcaggt
     6901 gtaggagcgg gaggagaata cggggcgtgt atgtccttga tttccgaaag tttttcaaaa
     6961 aaacaaattg gacgagcttc ttcggttgct ggtattggtg cgcaggtcgg tgcggctttg
     7021 gctgcgattt tagctgctgt gatcattcca acgtttggtt ggaagatgtt gtatgtcgta
     7081 ggtgtactgc cggtgttgat ggtcttgttc attcgtcgcg gcttgactga accaaaggaa
     7141 ttcaaatctg tacaatctgc cgaaaagaaa accaaactta gcagactgtt tgaatcaaaa
     7201 catcttgctt ggcaaacatg tggccttagt atcatggtaa ctgtccaaat tgccggctat
     7261 tttgggttaa tgaattggct gccttcaatt atgcaaaatc gattagggct ttcggtttct
     7321 gggtcttcat tgtggatggt cagtactatt ttaggcatgt ctttggggat gatgactttt
     7381 ggcataatca tggataaatt ggggcctaaa ctagcctttt ctttgttttt aatttgttca
     7441 tccttatctg tctatctact tgtgttagcc catagccagt ggagtttggt agcagccgct
     7501 gtgatagtcg gctactttat caatggcatg tatggtggat atggcgcagt gattagtagt
     7561 ttgtatccaa cagaaatccg cgcgacggcg aataatttca ttatgaacct tggacgagct
     7621 gttggtggtt tttcttctat cgtcatcggt ttcttgatgg atcattatag tttaactgcc
     7681 gtaatcttgt ttttgagcgg tatttaccta gtcagtctgc tggtcttgac aactcttact
     7741 ggtgttacag cattaaggga aagcttgcag cattgatagt tgagtctgta aatgacaaga
     7801 aagaggtgtg gcaaaagctt gcatctgcgg taataagacc agccaaatca aaaacatgag
     7861 acactgtttc cgtttggctg gtcgtattac ttgatgcagg gcagcttttt caactgctaa
     7921 tttccttcta tctggcggcg actattcttt gacgtccgtc atttcaaagt actttactaa
     7981 taacttagca taacgaaaaa ctgtggatac actcgtatat tgagcgtctg cataatcttc
     8041 caaatttttc tcaggatgtt gatacatgaa ttcaatcaaa tgataattat atgattttct
     8101 gtagaaatga agataaataa tgtagattgg gaagttctcc tgcttttcca tagaaaagcg
     8161 ccgattattc tcattcttca aaatgacctt atcaggatat ttttggaaca attcttctaa
     8221 atctgttaga taattcaaga tagacttact tgataccttc aattcgtttt gtagttcttc
     8281 tattgaacac caacgttctg tttcaagcag taaatgaatc attttaattt tcttttgaga
     8341 aacttttgtt aaaaaagtct gcatattttc tcctcctgta tctactttac tatactacat
     8401 acaagaaaaa aaacggatac aaatatcata ttgtcacacc gtcaatagtg cataaacctg
     8461 ttattatttc tctttttttc ctaagtattc tattgatatg aatcatttaa tcgtttatta
     8521 tataaatgtc aataatctaa
//
LOCUS       DAFCHQ010000103         8494 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000103 DAFCHQ010000000
VERSION     DAFCHQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.106"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="LNC71_002515"
     CDS             complement(<1..173)
                     /locus_tag="LNC71_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBK6467531.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LNC71_002516"
     CDS             complement(593..1714)
                     /locus_tag="LNC71_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBK6467532.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LNC71_002517"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LNC71_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBK6467533.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LNC71_002518"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LNC71_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBK6467534.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="LNC71_002519"
     CDS             complement(4304..5215)
                     /locus_tag="LNC71_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6467535.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LNC71_002520"
     CDS             complement(5232..6239)
                     /locus_tag="LNC71_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBK6467536.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LNC71_002521"
     CDS             complement(6236..8365)
                     /locus_tag="LNC71_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBK6467537.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="LNC71_002522"
     CDS             complement(8370..>8494)
                     /locus_tag="LNC71_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBK6467538.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAFCHQ010000104         8251 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000104 DAFCHQ010000000
VERSION     DAFCHQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.107"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            628..2721
                     /locus_tag="LNC71_002523"
     CDS             628..2721
                     /locus_tag="LNC71_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBK6467539.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            2881..3747
                     /locus_tag="LNC71_002524"
     CDS             2881..3747
                     /locus_tag="LNC71_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6467540.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(3769..4206)
                     /gene="ndk"
                     /locus_tag="LNC71_002525"
     CDS             complement(3769..4206)
                     /gene="ndk"
                     /locus_tag="LNC71_002525"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBK6467541.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            4865..7543
                     /gene="mgtA"
                     /locus_tag="LNC71_002526"
     CDS             4865..7543
                     /gene="mgtA"
                     /locus_tag="LNC71_002526"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBK6467542.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            7630..>8251
                     /locus_tag="LNC71_002527"
     CDS             7630..>8251
                     /locus_tag="LNC71_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBK6467543.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEG"
BASE COUNT         2686 a         1417 c         1743 g         2405 t
ORIGIN      
        1 tatcaagagg tcttgagcct gccgtttaac tctgagccac aaggagcaat ttcaaaaaat
       61 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      121 gtttaccaag aggttgtaaa attagcgtcc tgacataatc taataccgag ggtcagctga
      181 ttgaacaccg tgtatcaaga ggtcttgagc ctgccgttta actctgagcc acaaggaaca
      241 atttcaaaaa acagcttctc atgttttata aatattgtga cttgtggccg aggagttggc
      301 aggagaacac cgtttaagga agaagttgtg aaaaagctgt cctgcatcaa gaaataagga
      361 gctgcgacaa gaattttgtc acagcctctt tttttgtacg aataccgaga aactggcttt
      421 tgaataccgt ggagtgaagc ggtcaccaca agacttttat ctcttcatta gtttaatcgc
      481 ttacatcgca gcggttttat gctgtgaaaa gtggacggaa taatctttag aaagaataaa
      541 aaaatcttaa gataacaact tttttaggaa attttcattt ttcaggagca aaataagaaa
      601 aaatgtgaat agagagaagt gatatacgtg acacaactta aaaaattgtg ccaaaatcgt
      661 ttagcaatcg ttttgacagc tgttttcttt ttctggctga agacgatcat tgcctactat
      721 gcagattttt cattaggtgt ggaaggtacg atccagtact ttatcttgtg gatcaaccca
      781 attgcgacta ccttattgtt ttttggcctt tctttgtata ttaaaaagcc aaagccagcg
      841 ttagctattc ttttgatcat tgatatttta aatacgcttt tgttgtactt gaatattatt
      901 ttttatcggg aattcacaga tttcattaca gtcaaatctg tattaggctt ttcgaaagtg
      961 tcgcaaggat tgtcaggaag ctccttttct ttgatgaagc cccatgatgt catctactgg
     1021 ctggatatcg ctgtgttcat tgggctactg gtttggttga aggtcaagaa aatacctatc
     1081 aaaagcaacc ctgtcggcaa accgatggct atcgctgtta cctgtctttc agggcttatt
     1141 ttttcaggaa atctagcgct gagcgaagcc aaccgtccac aattgctgca gcgaacattt
     1201 gaccgttcat atattgtcaa atatctagga atcgatgcgt atactattta tgacggaatc
     1261 aaaacaggaa tgactagcag tgttcgcgcg catgcaagca gtaatgggat tgatgaagtt
     1321 ttagattata caaagaaaca ttatgcagag ccaaatcccg aaacatttgg gatcgctaaa
     1381 gggaaaaatg tgatcgttct ccatttagaa agtttccagc agtttttgat taatatgaag
     1441 gtagacggac aagaagtcac accgttttta aatagtattt tccagaatca agcaacgatc
     1501 agctttgaca atttctttca tgaagtagga cagggaaaga caagtgatgc tgaaaatatg
     1561 ttggaaactg gcacatttgg tttacctcaa ggctcattat tcacagaact tgggtcagac
     1621 aatgtctttc aagccgcacc agcgatcctt ggacaaaagc aaggctatac aagcgctgtt
     1681 ttccatggga atgtagctag tttttggaat agagatcatg tatataaaaa cttaggttat
     1741 gacaatttct ttgatcgatc ttattttgat gaatccgacg aaacgttagg ttatgggata
     1801 ttagataaag atttattcag agaatctgcc caatatcttg agcatctaca acagccgttt
     1861 tacacgaaat ttctttccgt aaccaaccac acgccttatt atacggatga caagaatttc
     1921 gactttccat ctttgaatac tggcaatagc acagtagata attatgttcg aacggcacat
     1981 tatctggatc aatcattgga acaatttttt acgtatttga aaaaatctgg tatttaccaa
     2041 aattcaatct ttgtgattta cggggatcat tttgggatct cgaatacaga taataaagat
     2101 ttagcaagcg ctcttggtaa agatccagat acatgggatg aatttgacaa cgcacagatg
     2161 caacgagtgc cattgatgat ccatatgcct ggttatacta aagggacagt caatcatgaa
     2221 tacggtggag aaatcgatgt tttgccgaca ttactacatc tattgggaat cgatgataaa
     2281 gattatcttc attttggtac agacttatta tcttctcagc atgatcaagt agttgctttt
     2341 cgaaatggga actttgtgac acccaaatac accgtcctag gaggaaaagc ttacaataat
     2401 cagacaggag aaatcatcga tacgaaagca gcaggaatcg atgaggaaat ctccaaagat
     2461 caggaaaaag tcaagactgc gttaagtctt tcagataagc tgaatcagga aaatctcttg
     2521 cggttttatg tgccaaacgg atttgagacg attgatccga aaaaatatga ctataaaaat
     2581 gaagcggaac gtaatgaagc aatcgaaaag caaaaaggcg aacattcgac aagtgtcttt
     2641 tcaaaaaatg gtaataaaac aacaaccaac ttatatccag taacaagaac agacgttgaa
     2701 cagcaaaaaa ataatcaata aacaatcaaa ccaaccttca gtagttagac aacattctaa
     2761 tgcttgaagg ttggtatttt ttatgaaaaa aacatttttt tatttttttc gtgtattttt
     2821 cctttttaac gtatctattt attgaatgtg gaaaaattta aattaaaaag gagaactatt
     2881 atgtatgatg gtgaattgat caagaaactt cgagtcaaca agaagttgac acagtcccaa
     2941 ttagcggaag gaatctgttt taagacttct ctagtcggga ttgagacaaa ttcagtgaaa
     3001 aaaatgtcat ttgtaacatt gagatcattt ttagagcgaa tgaacatgac acttgcagaa
     3061 tatgaatgga tgcgaaacca gctggatgaa ccaataaagt taaagaaaaa aagacgaatg
     3121 ctagatcggg tacaagaaga cagctttgat ccttataaag agatcagcaa taaccgtagg
     3181 cattttaaga aaacagaaga tctgtattat ttggttttga atcttcacat ggtcgaaaaa
     3241 acagaagtat ctgagacaga atttgaattt ttacgttatg aatgccgaat cattgaagaa
     3301 tacttgatgc agataccaga ctatggtcaa tttgaactat ctattctagc tgattttcct
     3361 tggatattca gtgatcggtt cattgagaac aattatttga aaatcaaaaa aagaatgcga
     3421 cgattagcac agttgaacaa tcatgagcag tatctcttta cttttttaat gaacctgact
     3481 tcgttttata tagaaaatgg tcggttgaaa aaggcacgta gtgtgaatga agatatgaaa
     3541 cggagcttag cacataaaga aagaagtaca gtgatctatg aaacattgat ggcagattat
     3601 tatcaaagat tgatttctgc tgctttaggg gaagaaaaga cgaaagacta ccaagaattc
     3661 ttcacagtgc tcgaatatat gttaggaaag aatcaacgga ggtccttaga gaaaaaactg
     3721 ctggccattg ctgaacccag caactagacc agcagtttag aatttgtatc acttcgccgc
     3781 tttttcagac ggtttgttta tatcgaatgg cgggaaaaag cgggcgatct cttttaccgc
     3841 agcatcacgg gaatcagaag cgtgaatgat gttttcagtc cccggcagcg cgtagtctcc
     3901 tcggatcgta cctggaacag catcggcagc ttttgttgct ccaaccatct tacggacgat
     3961 atcgatcact tcctcgccga ccagcacaag ataaacgaca ggaccggaag tcatgtaatc
     4021 gatcagttca tcaaaaaaac tgcgctcagt caagtgctga taatgttctt ttgctaattc
     4081 aggagtcata gtttcaaact tcatttcagc aatggccagt ccctttgctt caaaacgttg
     4141 gatgatgctt ccgactaaat ggcgtctcac gccgtctggc ttgataataa caagtgttct
     4201 ttccataaca gcacctcgtt tcctactata tttctattat agcgggaggt ggaaaatgag
     4261 acaaataaaa ccgcgtgaga caggaatata acgtgtgtat tttaaaaaaa gtatgaaaaa
     4321 catggaaaat tgatttgaca tcctcttttt tagttgttat attaacgaag gaaaaccgaa
     4381 taagatttgt taggtgaggc tcctatacaa acataggcta ctgcccaaaa acgtcgagag
     4441 acgccaatgg gtagaacagg aattgtcgag ataaggcttt tcataaggta gctaaaagca
     4501 ggtatctgct ttttacgttg tacagtgtca aaactcaacg atgagatctg agaagctttt
     4561 tttaatatgc tttttcaaac ctttcgctga gtatttggcg agaggttttt ttgttgccgc
     4621 aaatccccat cggtgaaaaa taaaaaggag gtggtctaat tggaaaaata actattcgaa
     4681 aaatgattgg aagtcatgaa ttaaaattac ttagtaaagg agatgagaac aaatggatga
     4741 cagtcattcg aaaaacaagg atctagagga aaggccaggg ctattatcta tctgttctca
     4801 gcccctttca gttttttctc tatcgatccg atgaatagct aaagaccaaa agcagcgaga
     4861 acagctgtct tttggacaca aaggagagat tgagatggaa aaaaagaaag atacacaaaa
     4921 aatctatgca gcttttgctg gaaaaacgaa acaagaatta tttgaactat tccatgcatc
     4981 agcaaatgga ttgacagaag aacaaatcga acgttcaaga gaaaattacg gtgaaaatgc
     5041 gatttcatat ggcaaaaaaa caccattcat tgtagaaata ttgaaagcat acatcacgcc
     5101 atttacatta gtgttgatcg cattaggaat tatttcattc atcacggaat atgtattagc
     5161 agctcctgga gataaagatc ttttcggtgt agccattatc ttcactatgg ttatcgtcag
     5221 cgggacaatg acgttgattc agtccgtacg ttccaatcaa gcggctgaaa aactgaaatc
     5281 acttgtgaaa gtaacggcag ctgtaaaacg taatggaaag tacacagaga ttcctatgga
     5341 agaaatcgtc tgtggagatc ttgttcggct ttcagcaggg gatatgatac cagcggatat
     5401 gcgtttattg tccgcaaagg atttattcat ttcccaagca gcattgacag gggaaagcta
     5461 tccagtagaa aagcatgcga aagtatgtga agacgatcag caaagcgaaa caagttacga
     5521 aaatcttgtc tttatgggaa gtaatgtcat cagtggaagt gcggaaggaa tcattgtttc
     5581 tgttggaaat gaaacactat ttggtcatgt agcagaaacg ctatctgaaa aaccaattca
     5641 atcaagcttt gaattaggca tccataaaac atccatgcta ctgatcaaat ttatgtcatt
     5701 gatggctcca gcagttatcg tcatcaatgg gcttacaaaa ggggattggc tagaagcttt
     5761 tctttttgga ctatctgtag cagttggtct aacacctgaa atgcttccaa tgatcgttac
     5821 gacgaatttg gtcaaaggag caagtgtgat ggccaaaaaa ggaacagtga tcaaaaattt
     5881 gaatgccatc cagaactttg gtgcaatcga tgtgttatgt acggataaaa cggggacatt
     5941 gacacaagat aaaatcattc ttgaatatca tatggattgt gaaggaaatg aagatgatcg
     6001 tgttttgcgc catgcgtatc taaatagcta ctatcaaaca gggctgaaaa atttgttaga
     6061 tgtggctatc attgatgaag caacagaaac tttagataca gataagatca attatcaaaa
     6121 agtggatgaa attccttttg attttgaacg tcgtcgtatg agtgttgtgg ttgctgatcc
     6181 agctggtaaa acacaaatga tcacaaaagg tgcgattgaa gaaatgctga acatttctaa
     6241 atttattgat cttggcggta ctgttacacc attaacaaaa gaacgaaaag aagccgtact
     6301 tgcaaaagta cagtcactta atgaagatgg cttacgagtg attggtgtcg cacaaaagac
     6361 caatccaagc gtcgttggag aattttcagt caaagatgag tcagacatgg ttttgattgg
     6421 ttatcttgct ttccttgatc caccaaaaga aacaacgaaa caagcgttga aagctctgaa
     6481 agaacatggt gtagctgtaa aagttctgac tggggacaac gaattagtta cacgttcagt
     6541 gtgccgtcaa gtaggtctag agatcaatga attggttacc ggtgagaaaa tctcggaaat
     6601 gagtgatcat gaattagcac aagtagctga agaccatgaa gtattcgtaa aattgaatcc
     6661 tcagcaaaaa gcacgtttga cgacagcttt acgtaaaaat ggtcatactg tcggattttt
     6721 aggagatggt atcaatgacg ctcctgcaat gaaagtggct gatatcggta tttctgtaga
     6781 tacagctgtg gatattgcga aagaatctgc agacgtgatc cttttagaaa aagatttgac
     6841 gattttggaa agaggccttc tttctggacg agaaactttt gggaatatca tgaaatatat
     6901 caaggctaca gcaagttcta attttggcaa tatgttttcc gtgctgattg caagtacgtt
     6961 cctgccattt ttaccaatgc tgcctttaca acttttgttt ttgaatctga tctatgatgt
     7021 ttcctgcatt tccttgccat gggatcggat ggataaagaa tatttggacg agccaaaaaa
     7081 atggaatgca tctagtattg gggaattcat ggtttacttt ggtccaacaa gttcgatatt
     7141 tgatattact acttacttgt tgatgtattt tgttatttgt cctgctgtag tgggcggaag
     7201 tttccataca ttggatagtt cacagcgagt gattttcatc gcattattcc atgcaggatg
     7261 gtttgtcgaa tcactatgga cacaaacctt agtgctccat gcactgcgta caccgaaagt
     7321 accatttatc caaagcaacg caacatttgc catgtttgtt atcactacat taggaattat
     7381 tgtcggttct gtccttcctt ttacaagttt tggtgctgaa ttaggcttga tgccacttcc
     7441 tggcaattac tggttatggt tatttgctac agtcatcgct tatctggcgc ttgtgacagc
     7501 tgtgaagaaa atctatatcc gtcgattcca agaactctta taaacgagaa ggctggttct
     7561 ttgcttctgt tctttttgga ccagtcgtgc taaactatgt gtattgtttt ggaaaggatt
     7621 aatgtataga tgaaaggtgc aatatttgat ttagatggtt tgttagttga cacagaaaaa
     7681 acgtatcgtg atggctggct ttggggattt cagcaatttg atatccatat cccaaaagaa
     7741 atcgttgacg cttggggcgg aaaaaactgg aaacaaagtt ttgatatttt agtgaaagca
     7801 gcaggtagcc ctgaaaaagt acaggaagtc cgagcgaagc gtgaagaata tttttatcaa
     7861 cagctgacaa gcggagccat ccacttaaaa ccatatgcaa aagaaatatt ggcagactta
     7921 aaagaacgtc atatgaaact aggcattgct acgaccactg ttacgaaaag agcaacagat
     7981 atcttggatc actttaaatt agctgattac ttggacacgt ttacattcgg agacgaagtg
     8041 tcggagaaca agccttcacc gattccttat ctgacagctt tggaacgaac aggattagaa
     8101 gctagttcag cctttgcagt ggaagattct ttagtaggag caacagctgc ttcacgagca
     8161 ggattaggcg ttgttttgat acctgattcc agttttgtcc gaaattatac cgtcgaagaa
     8221 aaggaaaaat tgaaacttct tgcggaagga a
//
LOCUS       DAFCHQ010000105         8248 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000105 DAFCHQ010000000
VERSION     DAFCHQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.108"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            234..1559
                     /locus_tag="LNC71_002528"
     CDS             234..1559
                     /locus_tag="LNC71_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBK6467544.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="LNC71_002529"
     CDS             1552..1902
                     /locus_tag="LNC71_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467545.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="LNC71_002530"
     CDS             1899..2069
                     /locus_tag="LNC71_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467546.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /locus_tag="LNC71_002531"
     CDS             complement(2973..3248)
                     /locus_tag="LNC71_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBK6467547.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="LNC71_002532"
     CDS             complement(3220..4173)
                     /locus_tag="LNC71_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6467548.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="LNC71_002533"
     CDS             4785..6278
                     /locus_tag="LNC71_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBK6467549.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="LNC71_002534"
     CDS             6413..6709
                     /locus_tag="LNC71_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBK6467550.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="LNC71_002535"
     CDS             6810..7661
                     /locus_tag="LNC71_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467551.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7965
                     /locus_tag="LNC71_002536"
                     /pseudo
     CDS             7682..7965
                     /locus_tag="LNC71_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2863 a         1283 c         1552 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat gtcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgac
//
LOCUS       DAFCHQ010000106         7760 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000106 DAFCHQ010000000
VERSION     DAFCHQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.109"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="LNC71_002537"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="LNC71_002537"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBK6467552.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="LNC71_002538"
     CDS             complement(2032..3285)
                     /locus_tag="LNC71_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK6467553.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="LNC71_002539"
     CDS             complement(3291..4214)
                     /locus_tag="LNC71_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBK6467554.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="LNC71_002540"
     CDS             complement(4435..6927)
                     /locus_tag="LNC71_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBK6467555.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="LNC71_002541"
     CDS             complement(6934..7401)
                     /locus_tag="LNC71_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBK6467556.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="LNC71_002542"
     tRNA            complement(7537..7610)
                     /locus_tag="LNC71_002542"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="LNC71_002543"
     tRNA            complement(7668..7741)
                     /locus_tag="LNC71_002543"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DAFCHQ010000107         7691 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000107 DAFCHQ010000000
VERSION     DAFCHQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.110"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            63..2390
                     /locus_tag="LNC71_002544"
     CDS             63..2390
                     /locus_tag="LNC71_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBK6467557.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2516..2845
                     /locus_tag="LNC71_002545"
     CDS             2516..2845
                     /locus_tag="LNC71_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK6467558.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2885..3889
                     /locus_tag="LNC71_002546"
     CDS             2885..3889
                     /locus_tag="LNC71_002546"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBK6467559.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3886..4332
                     /locus_tag="LNC71_002547"
     CDS             3886..4332
                     /locus_tag="LNC71_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBK6467560.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4482..5297
                     /locus_tag="LNC71_002548"
     CDS             4482..5297
                     /locus_tag="LNC71_002548"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBK6467561.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5503..6675
                     /locus_tag="LNC71_002549"
     CDS             5503..6675
                     /locus_tag="LNC71_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBK6467562.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6837..7331)
                     /locus_tag="LNC71_002550"
     CDS             complement(6837..7331)
                     /locus_tag="LNC71_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467563.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7328..7522)
                     /locus_tag="LNC71_002551"
     CDS             complement(7328..7522)
                     /locus_tag="LNC71_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467564.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2592 a         1446 c         1531 g         2122 t
ORIGIN      
        1 taaaaattaa atttagaata acgtgtaaag gattatttga tgatttttta ttgagaggag
       61 ggatgaaaat gattaagaaa actcaaaata aaatccaacg tatatgtcaa aaacattttg
      121 tttctagact caaagctgta tctaaaagaa aatacttaca tcccattatg atttcgatca
      181 tcttgatttt tactttctta gggagcttat caagtattta tgtaaacgtt tatacagaaa
      241 cgtcaaattt tgctaaccaa acgacagagg agacaaatag tccaccctat tctgaaatac
      301 agtccataga aagcaccaat cagtctagca acaacgagac aacctcaacc acatcctcga
      361 ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc aaaagcagca ggggagaaag
      421 cttattcaga aacaggtaga agccagacat ttagtactgt tgcggccact gcgaatttca
      481 acggacatac cataccagat ggatatacag tgatatatgc caaagatgga gtcattgttg
      541 cggttaacgg atcgatgagt gcggataaaa ctgcgttaca aggcgtaacc aaagcaatcg
      601 cttttattga taaccgtcca gatatccata atctgtttat tggctccaca gtaaatggta
      661 ctactatcac attcccaaaa agtatctata tgggggtggc taccgctgga aatacgatcg
      721 attcagttac acccaatgcg gataaaagtt attcttttac accctcaaca tggggagggc
      781 ttcctgccgg aggaggtatg agtacttttt caatcggtat acagttagca aatgatattc
      841 cattcacggg gtactcgatc aaaaatgggc agatcgttga tggtaaggga aatgtttcaa
      901 attttccaac ctatcaagca gcaacggttc aggtcaatgc gaatcggaca ggaacttttt
      961 cttatacagt caatggtgga gcagcaaaaa tcggaacgta cgggactaca ttatctggaa
     1021 tccatgtggg tgacaaagtg gttatcactc caacagcagt aacgggctat tcttttgcac
     1081 aaacaaacgg ttctttcaca gccctagaaa gtaacgtcga tacggtgacc tatacaggca
     1141 aacaaacacc aatcactatt aagtatcaag actattttgg acagatgatt gctccttcta
     1201 aaataatgaa tcttacttac ggaagtgctg cacaagacct gacgactaat acgcctacta
     1261 tcaacggata tacctttaca gctgtttcag cgatagaaac aaagaaccaa tcagcaatga
     1321 gtgttttagc tagtttagat acaaatggaa atgtaatcgt aaaggacgca aatggaaaac
     1381 agatttctga agtcatccta tattataaga caaatgcaac agtatcaatc aatgcaaatg
     1441 gcagtaagta ctatgatggt ctttctgttg taccgatcgt taaatatgcc tttaatgata
     1501 aaacagaatc aacatccctt ttaaacaatg atctcagtat tgatcaaatc acatggaatc
     1561 caactgattt caaagcgatg aatgaaaagg gagttgaagt aactgcgcct actgaaattg
     1621 gaacctatac gtcttggcaa ttgacaaaaa gcggactagc taaactagct gccagaacaa
     1681 actatttatt tactatcgtg cagacatctg atgtctatac gattaagcaa atcagcggtg
     1741 cggtagagct cagtggcagt aagacatatg atggaaaagc aggtgtcccg tcaatccatg
     1801 tcaagctagc agagggtgtc actagttcgc ttactccagt acaagtggca cttagcagta
     1861 tggattacac agcagatgca caatcagcaa aaaatatggt taatgctggc agctatacaa
     1921 ttaaactaac gaatagcgga atagacaaag tcaaagcagc aaatcccaag ttgtcattta
     1981 ctgatatagc caacacatca ggaacttata ccatctataa agctaacgca gtgattactg
     2041 tcgatgacgc tgcctttaat tatgatgccc aaagtcactc gatttcggtt ggaaatctcc
     2101 acgttaccgg tgttgtctta ggcgaatcat tggattatac cctaacagtt aacagccgga
     2161 cagatattgg atcacaaata gtaacgatta gtttaggcga aggaagtgta aacaataatt
     2221 atacgattat cgcaacagat accgctaagt tgacgatcaa tccgactctc ttgcttcctt
     2281 cgacaggtgg gataggtcta ctgccctttg tctttttagg actgatcttt attttttctg
     2341 gcttctttta ttttatccat cgtaagaaaa aagcaggtga acagcgatga aaaaaacaaa
     2401 aaatgtacgt gtggatatat ttgctgttct cttttttatc ctactggtct tttttatagg
     2461 ttctccgact gtgtcagcta gagaatcacc tattacattt actttgcata atcgaatgtt
     2521 cgaaagcgat aagatgccaa gtatcagtca aaacgacgga ataaatatcc cggataactt
     2581 tcaaggagta aaagggttga acggcgttac tttcgctata tatgatgtct cggatgaatt
     2641 ttacaagctg cggtcagaag gaagtagtgt ggaagacgca caaagaaaac tagcacaaaa
     2701 aagtgatagt gaaaaaatac tggcagaaaa tgtgacaaaa acagtagacg aggaagaggg
     2761 cattgcttct tttagtgcat cggacaaaga tgagcaaggt cgtgacgctg tttaccgatt
     2821 tacggaaata aagacttcag attaagtaaa agaaaaatca gcaccatttg tcgttgtgct
     2881 tcctgtgaca gattcatcta atcataaatt gacgaccatc cacttgtatc caaagagcga
     2941 acaaaaaata cctgctgcac tcacattaac aaaaacagta gagaataagc agacagattt
     3001 tgcagatggg gataaagtcc cttatctcat caccgtaaca attcctgaga acatcaacga
     3061 gataggaacc tatacgatca aagatacagc tgatcctcag ttatggctag aaccagaaac
     3121 gatcgatttt ttgattggtg gagacaagat tcatacattt cacacacaaa aaataaaaca
     3181 tggttttgtc ttatcagatt ctggaaaaga tcttagttcg tttgctggaa aaaaattaac
     3241 gatccgttac caaatgacat tgaaagaaaa cagtgaaaaa acgacatttg ataacgatgt
     3301 acgtttgacg actgacaatc aaacagtaga aacacatctt gccatccaaa caggaggcaa
     3361 acaatttgtc aaagtggatc ggcaaacgaa acaaaaacta gcagatgctc aatttctagt
     3421 caaaaaaaat gaccaatact tagcgcaaga aaacgggata aatcattggg tagccaaaga
     3481 tgattcaaga gcaacgattt ttacttccag taaagatgga acattttctg tccatggatt
     3541 gtcttttgga agttacgaac tagtggagat taaaccgcct aaaggatata tattgagtca
     3601 atcaaccatt ctctttgaag ttgaaaaagg cagttatgat ccgcttcatc ctataccatt
     3661 ggaaatcatc aacgaaccaa aagcaaatcc gacgaataat ggaaaaacgc cagttcagat
     3721 gcagacaaat ggtccatcaa atgaaaaaaa tggaggaagc tttccaaaaa caagcgaaga
     3781 gatgcttgga ggtttctcaa ttttagggtt attgctagtc ctttcaacag ggacagcttg
     3841 gttttataaa aaacaacaaa aaggaaacaa taggagggaa ataaaatgaa aaacatcaaa
     3901 acaaaaatat ggacgttttt aggaactgct atcatgctgc ttcctttcgt tctcggccta
     3961 ggaactgcag aggtttctgc agccgtatca cctacaccag aaaatgttac ggttaatttg
     4021 cataaattga aattcacttc agcaccagaa aatcaaataa acaacggaac ggaattgaca
     4081 tttccgaata gcgaaccgtt gaacggcgtt gaattcaacg tatatgacat cactgctacg
     4141 tactatccaa gtaaagacac agcagttcca gcagacgcta cacccttcgc aagtgtaacg
     4201 acttctggtg aaggattagc taacctgact cttccaggta aatctgatgg gaaagatgct
     4261 gtatacgtct ttgtagaaac accaaagccc ggtgtcgaaa cgtcgccaaa tatcgtgtta
     4321 agcttaccct agaccctgac aaacctttag acacgattca cctatatgct aaaaatgtca
     4381 tcaaagaaag caatatcaaa atccagaaag ctgggaactt ttcaggggta gataagttag
     4441 caggggcgca attccagatc aaaaattcag agaatcaatt cgtgaccggt tttgatgaaa
     4501 acactggtgt cgctctctat gatcctgctg agtcagcgtt gacattcaca actgatactg
     4561 aaggatccat cagtgtcaat ggattaatag acggcaatta tactttagtt gaaacaaaag
     4621 caccagatgg ttatcaatta accaatccac aaaaagaaac tacttttata gtaaaaaacg
     4681 gaaaattgca acaaacaacc ggtatcatcc atggcgcaga tgaagccaat cttgggaatc
     4741 caaacggtgt agtcaatcgg gaaaatacaa ctacggataa cacgattact gtagaaaatg
     4801 tccaaaacta cggtgatttc cacttcatca aacaagatgt gaacacgcaa gaaaaattag
     4861 ccgatgcaga attttctatt ctaagagcaa aagatccaac tgttaaatta atgaaaaaaa
     4921 tagcaaacct tgcctcagga gaatacccat atgcttggtc agatgaagtc acaggttctg
     4981 actgggaaga agtgaaattg atttctgatt cagaagggaa attttcaatc gaagggctga
     5041 aacaaggggc atactgcctt caagaaacaa aagcaccaga aggctacgca ttacctaaga
     5101 atgaagctgc gtacactgaa tttacagtag atgatgatcc aacaacttct gatgaaacaa
     5161 cgatcgataa ccaacctaaa ggaatcttgc cttcaactgg tggtatgggg atcatcgtat
     5221 tcatcttggt aggtactgct ttagttggag gagcagtgat ctatttcaaa aaacgccatc
     5281 aagaaacaga agcttaaact attggaaaca aaaatcatac gataaataaa cgatagtgat
     5341 tcaaatgggg agaaaggttg tgacaagtgg tcggtctctt ggtattcgct caacaaacgg
     5401 caaaatagga ggaactattt tgccgtttgc ttcgctaata ctcaggtcaa ggcgctgatt
     5461 tcacaacctc ttcatataca tattagaaaa taggtggaca tgatgaataa aagcagaata
     5521 agaatactgg acatcttcat ggcaatcatt ttagtcgttg ggatcgggat cttttcttat
     5581 ccttttgtag aagattcact gaatgatttt cttgcccagc aaatgatcat ccattatcaa
     5641 aagcaagcat cgaataagaa ttcagaggaa ataaaaaaac agcaggaaaa aatgacaaag
     5701 aaaaaccaac agttggcaga gcaaaacgta tcaccaggga taggcagttt caaccaagca
     5761 gttgatgcaa aagcattaaa agatttacct agtaatgcct tttttatgga acatatgctt
     5821 ggagtcatcg aaatcccaaa aatcaatgtc agcttaccta tattcgatca gacaacggaa
     5881 atatttttgc aaaaaggaac gtcattattg gaagaaagtt cttatccgac tggcgggaaa
     5941 aacacccatg ccgttctttc tgggcatcgt ggactcccag aagcaaaatt atttacagat
     6001 ttgcctaagt tgaaaaaagg agaccagttt ttcatccaga tcaatggaaa gacattggcg
     6061 tatcaagtag aaaaaatcca agtggtctta cctgatgaag tagattcttt gggtatccaa
     6121 aaaggcagag atctcgtgac tttgcttacc tgtactcctt atatggtcaa tacacatcga
     6181 ctgcttgtga cggggcatcg tattccttat caagcaaaag aggcaaaaaa agcgatccaa
     6241 ggaattgatc aatggaaaaa atggaaattt tttatttggt ttatcggtat actacttgga
     6301 tcaatcggct tagtttggct actcatcgct tatttagatt ctttggccat cgctaagcga
     6361 aactatcctt tatcttttta tgtaaagaac acaaacggac gaccaatcga aggaatggtc
     6421 ttttctgtaa aaacgctcaa cggcaaacac tatattacta gagaaaaagt tccatttgtg
     6481 aaagcttctg atgaatacgg actagtgaga ttttctgatt taaaaggcag gaactatcgc
     6541 ttgcaacatg aggagctcct gcttaaaatc catgtaaaac ataaacattc gaaacagttt
     6601 tcaatgaaat tgaaaaaagg aagatataaa ctgcgaaaag aaaaagaggt gtactatttg
     6661 attgagaaag aatgaatgaa gatgtccaaa aaagcagagg gaacagacta aatgaaaaaa
     6721 gagagaagct gacgaaatgg acgtgtaaac tgttattttt ggtttactat ctggaacgcc
     6781 atcttctatt tttttatgtt tcagaagaat tttattatta tagtataact tttgaattag
     6841 tttccataac atttggagca aggggttaaa ccacgagctt gagcttcgct taaagtggca
     6901 ggggtatacg taccatttcc acatttatgt gtatggtatt ttgaaccggt cggtgtgaca
     6961 tagacagttt gttcattatt ttgctcagga gctgcggctt gtgcctgctg agaggcctgt
     7021 tcagctgctg attgctgtcg cgcctgttct gcttgttgct gtgcgattgc ttcttgcctc
     7081 gcctgttctg cttgttgctg tgcgattgct tcttgttgcg cttgttctgc ttgttgtcgt
     7141 gcaatttctt cttgcttttt ccgttcttct tctgcttctt ttgctttgat tgtggaatca
     7201 attgcagcaa gacggcttgt caactcttgt ttgttttcag gtagcgcttg gatagctgct
     7261 gaagcagttg catagttttc ttttgtagga tttgcttcag cttgtgacaa agcagattcc
     7321 gcttctgtca ctttttttct agtgcagctt tttccgcttt ttgtttctct tcttttttct
     7381 tttcaatttg ttcttgttct ttcgatgaat ctgccgtact ttcggagatc gtggtactgc
     7441 tgctggaagc ctgatctgaa cttgtgcttt gagtagtaga cgtagtagca ccacacccac
     7501 tcaacaaact tcctgcgacc aaaaacaatg caactaattt tttcgctaaa tttcctcctt
     7561 aagtaaaaaa caaccgtttt caataaatgt ccttttcaat tgtttactta cttgttataa
     7621 taattgtgtt atgaatagtg attgaggaaa cttaatctaa tcccgtgttg atacacggga
     7681 ttttttgcta t
//
LOCUS       DAFCHQ010000108         7600 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000108 DAFCHQ010000000
VERSION     DAFCHQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.111"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(942..1355)
                     /locus_tag="LNC71_002552"
     CDS             complement(942..1355)
                     /locus_tag="LNC71_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBK6467565.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="LNC71_002553"
     CDS             complement(1811..2296)
                     /locus_tag="LNC71_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467566.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="LNC71_002554"
     CDS             complement(2435..2650)
                     /locus_tag="LNC71_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBK6467567.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="LNC71_002555"
     CDS             2874..3674
                     /locus_tag="LNC71_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6467568.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="LNC71_002556"
     CDS             3693..5561
                     /locus_tag="LNC71_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBK6467569.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="LNC71_002557"
     CDS             5554..6045
                     /locus_tag="LNC71_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBK6467570.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="LNC71_002558"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="LNC71_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBK6467571.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="LNC71_002559"
     CDS             6605..7600
                     /locus_tag="LNC71_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBK6467572.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga
//
LOCUS       DAFCHQ010000109         7583 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000109 DAFCHQ010000000
VERSION     DAFCHQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.112"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..339)
                     /locus_tag="LNC71_002560"
     CDS             complement(<1..339)
                     /locus_tag="LNC71_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467573.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..760)
                     /gene="tnpA"
                     /locus_tag="LNC71_002561"
     CDS             complement(356..760)
                     /gene="tnpA"
                     /locus_tag="LNC71_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBK6467574.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            941..2095
                     /locus_tag="LNC71_002562"
     CDS             941..2095
                     /locus_tag="LNC71_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBK6467575.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2089..2511
                     /locus_tag="LNC71_002563"
     CDS             2089..2511
                     /locus_tag="LNC71_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467576.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3645..4613
                     /locus_tag="LNC71_002564"
     CDS             3645..4613
                     /locus_tag="LNC71_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6467577.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            4623..5111
                     /locus_tag="LNC71_002565"
     CDS             4623..5111
                     /locus_tag="LNC71_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6467578.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            5407..5688
                     /locus_tag="LNC71_002566"
     CDS             5407..5688
                     /locus_tag="LNC71_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467579.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            5690..6106
                     /locus_tag="LNC71_002567"
     CDS             5690..6106
                     /locus_tag="LNC71_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467580.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            complement(6861..>7583)
                     /locus_tag="LNC71_002568"
     CDS             complement(6861..>7583)
                     /locus_tag="LNC71_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6467581.1"
                     /translation="HRQPKGIIKSATISRHSSGKYYISLLCKEEIIELPKTNSTIGID
                     LGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQ
                     KRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSW
                     SKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHD
                     RDINASINILTEGLRLHSMGLA"
BASE COUNT         2682 a         1312 c         1237 g         2352 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaaactt ttagaccaga acatttcttt ccaaagaaat tgtttgactc
      481 ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg
      541 tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc
      601 actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat
      661 cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat
      721 aaagaagaaa tactgaatga ttatttgtat ctaattccat gttatatcaa ctcatttctt
      781 gtatagactg attgtaacat ggaacaaaat aaaaaggcaa tacgccttag ttgtcggtct
      841 tcgagttacc aacggtaacc ctcataccga acggcattca tcacccacct atagaggatg
      901 ggcgacttct gcctaattct gttaaattta acacaaaggc gtgattattt tgaaacccaa
      961 aaagaaaatt tatctaggtc tcctcttttc cttgatgatt ggaattttta gtataggaat
     1021 tacaaatacc tatgcttcag aaaattctaa tttcaatatc aactcccaac caattgaaga
     1081 acaagcaatt aattttgcga aaactgattt tgaacactcc ataacttcaa ttcttttaat
     1141 ggaaaataac accgaaactg atattaacta tactcttggt tcgccattta aaatgctcga
     1201 tggagaaaat acggcaaata gttctatttt ctttccagta ttttcagacg ctgataatac
     1261 gattaaatat gtttataccg tcaaaaaaaa tacaaatgga gaatattcag gaagtatttc
     1321 acaattttta gcaaaagaac ttcaaaatct agtagatagt aatataacag atacaacctt
     1381 attttttcaa gataatagca tttattattc cgataatgga caaattaaac tactttggaa
     1441 atcaccttta agtacagaaa aaaatggtgt ttcttccctt acagaaaaaa atgatcttat
     1501 atccgtaaat ataaaaaata aaattaaaaa tttttctaaa aaacaaaatt ttcctgcggt
     1561 taaacgtgta aaaagtgtct cctccggaga taactttatt atgattaatt ggcgtatagc
     1621 agaaacccaa acaagttttc cgtggtgcgc tgcttacgta gaagctgcta tcttatgtaa
     1681 caaaacagat gtacgtgcta cttcagctag agccattatg cattatacct atcctaattt
     1741 aaacaacagt caaattgaat caaaaagtct taatcaagat caaataataa aatatgcaaa
     1801 ttactgtggt gtttatccta aaaaaatagc acgaatacta aacctttcag aggtccaaac
     1861 acaactaaga aaaggaaatg ccatttatat gggaactgct ggttatggaa attatagtaa
     1921 cagccgtcat gcacttgcat tatttgggtg ggtcaatcaa ggtggttatc aaacttacta
     1981 tgtgtggaat ccttggtata actacccaac tgtaataagt gcaaatgcag gaccagttac
     2041 aattcctgta cctggaggtg gttttacttg ggacaatacc attacaaatt ggtaaataaa
     2101 ctcattgctt tagttatggg tctagtgtta gttcttttct taactagttg ttcaaaaaaa
     2161 caagaaatac aaaagactga tctgattcct tttctaggaa cttggcaaac taaagatcat
     2221 tcagaacgtc tagtaatcac taatttaaat acttcaaaaa atatagaaca atataccatc
     2281 ttagtaaaag agaacaatca taaaattcaa atcattgata aaaatttaaa tataaatgaa
     2341 caagttttca tttacaaagc aacaaataat ttactaacac tgcgctcgaa taaaaaacaa
     2401 ttagattaca cttttagttt ggattctcca aaaaagctcg tatttatttt gacaacgaat
     2461 gatgggacag aagggacagc taaaccaatt gaatttttta aagctgattg atgaatagtt
     2521 gtttgatatt acaaaacaat aatttcaccg aaggtgaaac aagcgaaact ttacaaaaag
     2581 tggctagcat ttgctagcct tttttgtatt gtggagcgtg ggttttgagg gggattcaag
     2641 ggggagcttg cgcccctttg acaagcggtt atttgcacca aatacgtagg tatttggcta
     2701 gcaaatatag agcttgccaa gccctttagt gtgttttgta gaaagggacc ttatagtcac
     2761 tttcacatta caaacgatac gtcctatgga cgtatcaaaa gggtgggact tatgagcaaa
     2821 ttaaagcaaa atttggctag caatccttct aaaaaataca cctattgatc agagtcaaaa
     2881 taaatcagtt ttcgagtgag cgaatccaaa tttgcaactc tgaagctttg caaatgagtg
     2941 tgccggcttc cgtaaaagtc taggtaacat tagtcgatat atacataggg ctctaatttg
     3001 cgttttaagg cgttttaaac gttctggtat gtaattatac caaagcgact ttaaaacgcc
     3061 ttaaaacgaa ttttagaggt tctgtagaac gttttagggt tgttagggct tgccctgacc
     3121 gcctgtaagg cgctttaaaa acaagataag ggattttatc tagacttgtc ttaaaacgcc
     3181 gtagatgagc gctcagagct tctaaaagga ctccttacga actgttttta agcaaaggag
     3241 cctttaggcg actgcgcagt ttaatgtgag agcgtagcga tcacaccttt aaagcgaccg
     3301 tagggagcag attcccagtt taggaaattt cgcgaacctg tttggagaaa aaaagtagga
     3361 aaattttagg cgggaaattt tgcccccctc ttttataatc tcttttataa cctcttttta
     3421 aacctcttta ccgtcggctg tatccttact ctcccaaggt gtgcagaatc ttttaggggt
     3481 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     3541 aggggtcttt ttgtctgttt ttaggggtct ttaattaggg ggttgacccc cattagaaag
     3601 ctctttataa ttttgttaag tagttaatct tgaaggagga ttttatggca aatgaaattg
     3661 tgaaatacca ccatgaatta aatacgattc ctttacgcaa atttacgtca gtagaaatga
     3721 atttattctt ctctataata tctagaatgc gtgatgtagg cgataaaaaa gtacaattta
     3781 cattcgaaca attaaaagat ttaagcaatt ataaagcaac tgcaaatgtt cgattcatag
     3841 atgatttaga aacaacgtat gacaaattaa tggatttacg ttttggtaga agaagtgctg
     3901 acggtttgca acgtgaacga tttgttttat ttaatcaatt taaaatcgat ggaaaagctg
     3961 atattccttt tgctgaaata caagtccatg agaaagcctt acccttactt aataatcttg
     4021 aagaatgggt tcgatattct ctccaacaat tcaatgaatt agaaagcagt tattccaaaa
     4081 ccatgtttag attattaaaa caatacagaa ccaaaggaat tgccaatttt tcaaaagaag
     4141 actttcatga attacttgat atccctaaaa gttatagaga aagtgatata aataaaaaaa
     4201 ttataaagcc tattcgccaa gaactaaccg caatcttcaa aggtttgaca atccaaaaga
     4261 gatacggaaa aggacgggga aagcctgtca ttggctatca atttacattt aaacctgaga
     4321 taaaaaaatc agacgacttt aacaaacatg ctccaaccaa agaagaacaa aataaagctt
     4381 tggaactagc taatcaacag gttcaatctg aaaaagaacc tacttcatta caagaaaaaa
     4441 tcaaagaaca aaaacgaaaa ttagaacttt tacaacagtt agcagaatta gaaagaaaac
     4501 aaaatacccc aagtaagaaa attaacaaac aaatagcaga taacaattcc gatattaccc
     4561 cagaatttaa agaaaactta ctagataatc tccaaaactt atttaaagaa taaggggttt
     4621 ttatgacaga taaaactatc aaagaattag cagatattct aggtgtttca aaagataaag
     4681 tgaagtatca actgaggaaa ttacctagta attttaccta caaaaaagga aatattacct
     4741 acctgaaaaa agaagcagta acaacaatta caaaggtttt atcaggtaat gaatcgggta
     4801 acttacctag tgaattaccc gattcattac ctaccgatta cctactcaag gagattgaaa
     4861 ctaagaataa tcatattgaa gaaaaagata ggcaaattga aaaacttcaa atagctttag
     4921 aaacgcagca aaaattactc gaccagcaac aacaactcac cctacaagcc aatcaacaaa
     4981 tcaaaaaact tcacgaacaa ctccagctaa cctatgcaga agaatcttca gaaaaagaac
     5041 ccctttctgt tactgaagaa actcaaaaag gacaaaacaa ggagaaaaaa tggtggcaat
     5101 tctggcaata acccaacaat ttttattcat aaaaattgtt gggttacttt ctttttttgc
     5161 ttttacaagc atagcgacat gatctcacgt cgctattttt taaagagcat aaggaaacgc
     5221 ggtatcactt tcttacgaaa ggtgctaggt aggctgtgcc taccacgttt ctaagaaaaa
     5281 aatttgctat cactttttcg tcattgtgga tagcttatgt atagtgtttc ggcaaaaaac
     5341 agctatacaa atactatatt tgtatgatat acataaatta tacacaaatg cgaaaggaat
     5401 tcttctatgg acattttagt tcggaatatt gatccaaaag ttatttcaga aatagataga
     5461 cgttgccaag aactcacaac aaaaactggc aaaaaatgga gccgtaatgc gtacctaaaa
     5521 gttctaatcg aaaacgattt tgaccaggca cttaggcatt acaaaaaaga tcaattcgat
     5581 ttattactcg aaaaatttat cgatatacaa gcaaaaaata ctgaaacatt gaacgactat
     5641 gttcatacaa acaaccaact cattgaaaca cttatcggag gaatataaca tgagaaaaac
     5701 ttctgttcaa aaacacgtta ccctttatcc cagtcacatt gacgttttag attccatcat
     5761 cgaaaaaaat ccagatatca ataactactc ggaagccatt cgttttctat gtatgagtta
     5821 tgaacctaat actaatacga caactaccag caaccaagca aaactaaatg cgatgggaaa
     5881 agaaatttct attatgtccg aaatcattgc agaatttgct gatttatatc taaaagatac
     5941 tggtgttctt atcggtacaa attctaaagt atatcaagaa gtgaaagaaa aaattgaaaa
     6001 tcaactcaat ccctcccgac gaaatcaacc caaaaatcgt caaaaagaaa ccaatcaaac
     6061 attcacttct agaaattttg acccaacaaa ctttctaaaa agatgaaaaa gaaaccgaaa
     6121 attttttcta tttctcagtt tcttttttag ggggcagtct tccagcaaga tttgcaagtt
     6181 accggtaact tgcacaaaaa aagaaaaatc cgcttgtgcg aatccttctt tttgcatttt
     6241 ggggcgctgc cccaatctcc aaatatcggt tatttaatag cgtggtaact caaaaaaata
     6301 gaacgtactc tgaaataaaa aaagactgct ctaacgagca gccaataaaa aataatgagc
     6361 gtttattctt gaaaaagcag cgcgcttatg aagcgctgcc aaaaatttac catattttat
     6421 gaaaaataac aagtttctaa aaaggaatcg gttaagattc acaaagaatc gtaaacagac
     6481 aatagcggaa aatcaagctt ccgaacgtac cttttagcta gaccaatgaa aaatcacaaa
     6541 gtgagcccct gtatcggttg atacaagggc tacttggcat actcgacatt atgtctacta
     6601 gctcgataaa taacgattat gttcccaaaa caatatttta tgaactataa caaaaatttg
     6661 ttatcgcata ttgtatattt taattatagg tgtatatcac gtgaactact caccacttag
     6721 ctaaacctga cggttcttaa cgcttgaagt gggagcttct tgggaataga gcatacttga
     6781 tagcgtattt ctttactaac agcggtgtct cttatttacc aagctatccc cgtagttcct
     6841 acggttcttg atgattggat ctaagccaat cccatagaat gtagtcttag accttcggtc
     6901 agaatattga tactagcatt gatatcacga tcatgatgag tatgacaaac aggacaagtc
     6961 cattctcgaa tttcaagaga tttcttgcca tctttatgtc cgcactctga acaaatctga
     7021 ctagacggaa accatttatc tactttgatg atttttcgtc cataccagtc agccttgtat
     7081 tgtaatttag tcacaaactt tgaccaagaa acatcagaaa tacttttagc taatttatga
     7141 tttcgcaaca taccttttgt gtttaagtct tcaatacaga taatatcgtg atttttgatc
     7201 atttctgtac tcaacttatt cagaaaatca gtacgttgat tcattacttt ttcatgcaat
     7261 ctagctactt tgcgtttttg tttttgataa tttttagctt caaatagatt ggtaccttta
     7321 tttttagcca ataaagcacg cctagacaat ttgcgttgtt cacgctttag tttcttttcc
     7381 attttggacg tgaatttatt attatcaatt ttttgtccat cagaaagaat cgcaaagtcc
     7441 ataataccta agtcaatacc aattgtggaa ttagttttag gtaattcgat aatttcttct
     7501 ttacacaaca aagaaatata gtatttaccg ctagaatgac gtgatattgt agcagatttg
     7561 ataatccctt ttggttgtct atg
//
LOCUS       DAFCHQ010000110         7537 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000110 DAFCHQ010000000
VERSION     DAFCHQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.113"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..1248
                     /locus_tag="LNC71_002569"
     CDS             join(107..335,335..1248)
                     /locus_tag="LNC71_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6467582.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="LNC71_002570"
     CDS             1483..1935
                     /locus_tag="LNC71_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467583.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="LNC71_002571"
     CDS             1949..2638
                     /locus_tag="LNC71_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467584.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="LNC71_002572"
     CDS             2763..5114
                     /locus_tag="LNC71_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBK6467585.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="LNC71_002573"
     CDS             5194..5589
                     /locus_tag="LNC71_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467586.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="LNC71_002574"
     CDS             5914..6531
                     /locus_tag="LNC71_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6467587.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="LNC71_002575"
     CDS             6572..6871
                     /locus_tag="LNC71_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBK6467588.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="LNC71_002576"
     CDS             6905..7072
                     /locus_tag="LNC71_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6467589.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="LNC71_002577"
     CDS             complement(7117..>7537)
                     /locus_tag="LNC71_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6467590.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAFCHQ010000111         7436 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000111 DAFCHQ010000000
VERSION     DAFCHQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.114"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1087
                     /locus_tag="LNC71_002578"
     CDS             <1..1087
                     /locus_tag="LNC71_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6467591.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="LNC71_002579"
     CDS             complement(1305..2447)
                     /locus_tag="LNC71_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6467592.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="LNC71_002580"
     CDS             complement(2452..2637)
                     /locus_tag="LNC71_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBK6467593.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="LNC71_002581"
     CDS             complement(2991..3236)
                     /locus_tag="LNC71_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467594.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="LNC71_002582"
     CDS             complement(3230..3631)
                     /locus_tag="LNC71_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6467595.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="LNC71_002583"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="LNC71_002583"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBK6467596.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="LNC71_002584"
     CDS             complement(5649..7415)
                     /locus_tag="LNC71_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBK6467597.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         2383 a         1429 c         1026 g         2598 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttatt
//
LOCUS       DAFCHQ010000112         7234 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000112 DAFCHQ010000000
VERSION     DAFCHQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.115"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            147..1079
                     /locus_tag="LNC71_002585"
     CDS             147..1079
                     /locus_tag="LNC71_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467598.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1300..3063
                     /locus_tag="LNC71_002586"
     CDS             1300..3063
                     /locus_tag="LNC71_002586"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBK6467599.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3147..3605
                     /locus_tag="LNC71_002587"
     CDS             3147..3605
                     /locus_tag="LNC71_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBK6467600.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3714..4397
                     /locus_tag="LNC71_002588"
     CDS             3714..4397
                     /locus_tag="LNC71_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK6467601.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4544..5158
                     /locus_tag="LNC71_002589"
     CDS             4544..5158
                     /locus_tag="LNC71_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBK6467602.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5174..5380
                     /locus_tag="LNC71_002590"
     CDS             5174..5380
                     /locus_tag="LNC71_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467603.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            5921..7051
                     /locus_tag="LNC71_002591"
     CDS             5921..7051
                     /locus_tag="LNC71_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBK6467604.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT         2473 a         1142 c         1481 g         2138 t
ORIGIN      
        1 tttttaaaga aaatgattat ttgaaaaata aagaaatcat tatcccagaa tttgactcgc
       61 ggaacatctt gctagcttta gtacgttatg tcaattggga aggctatgaa aataaaaatc
      121 cgtatgtaga agaagcttgt aacgagttga ttgattatgt agagactcat ttttttaaaa
      181 gaagatattc ggaagatgaa aagtccttta tcatacgagg aatagagatc gcaatcggaa
      241 gaaaggcatt tccccttgga ctgaaagaag aagataagaa aagcgcagaa acgaagccgt
      301 tatttcaatt agtctgtcaa ggattggaag agcaaaaagg aattctagac ttacaagaag
      361 aagatgtcta ttatattttt tctttgttca acagtagaaa ttataccaat gagaacatgg
      421 agctgttaag aaaagatttc gaagttgttt ataaaaattt tgttctcaat gacctatcac
      481 tgaatgaatt gattttacag attattagta tcgctgaaat cgacgatgca gaaaatttta
      541 ttttcaagca atccttttta caatttgtcc gtacgttgtg ggcagatgga caagtatttt
      601 taccggaaag gatctatctt ctatcagaaa aagaacaact tctgtacgga aaaattgtag
      661 aaatattgga agagtggaaa aagaaaaatc aaatccaact aagatggaat gaaaatttca
      721 ttcgaaaatt cgtcaaaagt ctttctttga tcaacagtga acaagcgggg agacaggaaa
      781 tagaaatttt tgttgtttca gaaagttcag tcaaacaatt tttttatcga acccaatttc
      841 atttgtatgc ggaagaaggc atcgctgaaa taaatcctat tttatttcgt tcattagaag
      901 aattgcctga tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat gctgctagct
      961 atcaaaatgg attagaaact gaaaagacaa agatctatcc aatctcatta aaaggtgcaa
     1021 acatccattt gtatcatatt gctcaggaac ttcacctatt aaaaaaacat atttcttgat
     1081 gagaccttaa ttaggagacc ttctacaaag ctttttaatt gtctgtagaa ggtttttatt
     1141 tgagtcctga ttcgtgcgaa gatctactta tataacaagc tttctctgaa atttttttaa
     1201 aatgctattg acttagagtc aactctaagt agtagaatag ataaagtgta acaaatctat
     1261 tttcattaaa agaagatagt acggaggaga gaatgtatta tgagtacagt attacatgta
     1321 ttggatgcaa tcaatagccc cgcagatgtc aaaaaattat ctgttgagga aatgaaacag
     1381 ttggcagaag agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg tgggcacgtt
     1441 ggtccgaatc taggtgtagt agaattaacg attgcgtttc attatgtatt tgattcacca
     1501 aaggataaaa tcgtttggga tgtttcccat cagtcttatg gtcataagct tttaactggt
     1561 cgaaaaaatg gttttgaaaa aggacattac cgtgaagtga gcggatattc caaccaacat
     1621 gaaagtaaac atgactattt tacagtgggg cacacatcta catctatcag cttagcaaca
     1681 ggtttagcta aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc atttatcggt
     1741 gacggctccc taagtggtgg cttagctttt gaaggattga acaatggtgg tgttttacaa
     1801 tccaatctaa tcgtattagt aaacgataat gagatgtcta ttgatgagaa tcatggcagt
     1861 ttatataaaa acttagctga gcttcgtcaa accaacggta aaagcagcaa taacctattt
     1921 aaagcaatgg gatttgatta tcgttacgtg gataatggaa acgatctgga aacggtgatc
     1981 cgtttatttg aagaagtaaa agatattgat catccgattg tacttcacgt ccatacggaa
     2041 aaaggtcgcg gttatcagcc ggcaatcgat aataaaatga aatatcattg gcatgtacca
     2101 tttgatctgt ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt taatatgatc
     2161 atcatggatt atttggataa gaaaatgcaa acaagtacag ctcctattgc cgcagtcaat
     2221 gcagcgatac caggcgcctt tggactgaaa acattccaag aaaaacatcc agatcattat
     2281 tttgacgtgg ggatcgctga acagcactct atcactttcg cttctgcttt ggcttctcag
     2341 ggagtacgtc cagtcgtctt ccaatcaagt actttcttgc aacgggcata tgatcaatta
     2401 tcacatgatt tagcgatcaa cgagaatcca gtgattattt tagtcggtgg cggaacgatc
     2461 tcagctagtg accgaacaca ccaaggaaca tttgacattc cttacttatc aagtatcccg
     2521 aacatcaaat atcttgctcc agcaacaaaa gaggatatga tctctatgct agattgggcg
     2581 ttcgaacaaa cagcgcagcc tgtagcaatc cgaattccta cacatggtgt agaacatggt
     2641 acagctatcg caaatgatta tagtattcca gcctatcaaa cagtaaataa tggaggaaaa
     2701 gtagcaattt tagctttagg cggttttctt gaattaggaa aagaaacagt aaaagctttt
     2761 agaaaaaaag gaatcgaagc aacgttgatc aatccaggaa cgatcacaga aaatgacgta
     2821 gaaacattag ataaattaaa agaaacacac gaattagtca tcacattaga agatggtagt
     2881 ttgtcaggag gatttggcga aaatatcagt cgttattatg gtgattcttc aatgaacgta
     2941 ttaaattttg gtgcagagaa agaattcacg gatagcgttc ctgtagggca gctttatgaa
     3001 cgttatcatt taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt gaatattcaa
     3061 taattcatta tgctcaccat ccagataatt gtttatacta gtatctggat ggttttttca
     3121 ttataaaaag caaaggggtg gatgttatgt atcagcaagt agaagaatat gtcaaaaagt
     3181 tccaagaaaa atatcctagt tatcaatcag aaaaattagg tttcttttca tttggggata
     3241 cagaaaaaat ggcagacgag ttgagcgaac tagttgtaaa aaagataaag actggtacat
     3301 caagtggtta cgatctgtat aaacaagaga atgagccttt accaaaagtt ggtcaattag
     3361 acgtggtcct gaatggaaaa aatgaaccag tttgtatcat caaaaatata aatgtgacca
     3421 tcttgccttt tagtgaagtc acgaaagagc atgcttggaa agaaggagaa ggggatcgga
     3481 cattagctta ttggcggaaa gtccatgctg agttttttaa acatgcttat aaagaagcgt
     3541 tgaacataga atttacagaa gagaagctgg tcgtatacga agaatttgaa gtaattttct
     3601 tttagtaaga aaggtctgct gaaagaaata gctcattatt gtctcctgaa tagaaaaggg
     3661 ttacattttt atatgaagct gtatttttat ataacattac tggaggagat attgtggata
     3721 cactggaaca aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa aacgagccgc
     3781 tacacagttc ttttattatg gggagaatgg ctttggctgc aaaagaaggc ggagcagttg
     3841 gtattcgagc aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt gatttaccgg
     3901 taatagggat tatcaaaaga gattacgatg ggtcagaagt gtttattact gcgacagaaa
     3961 aagaagtgga agaacttttg gagacgcaag cagaaatcat tgcactggat gcaacttctc
     4021 gtaaacgtcc gacagaaaaa acaactgctg aacttgtcca aatgattcac gaacacggtc
     4081 ggttagctat ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa gaagatggtt
     4141 ttgatctttt gtctacgact ttagcaggct atacagaata ttcacgaaag acgcagacac
     4201 ctgattttga tttattgaat caaatcatag aacaagtcga tatcccagta attatggaag
     4261 gacatactga ctctcctgaa caagtagaga aagcatatga attaggtgct ttttcagtcg
     4321 tagttggatc tattatcacc cgtccgcaat tgatcactaa aagatatgtc gaagccgcaa
     4381 gtaaagcaaa taactaaaaa acacgcacgg cacagattct tgacaaaaaa gaaaaattga
     4441 tagatagttt aaagcagatt ctaagaagat gaaaaaagca ttacttttca ttttgtcttc
     4501 aagatctgct ttttaacgaa ttttatagaa agaaggcgaa accatgtttg aacggagtcc
     4561 ttcagaattg acagaacgtg aaaattacaa gttgctgatt ggttcagtca tccctagacc
     4621 agtagcagta gtaacgacta aatcagcaaa agatatagtg aatatcgcac ccttcagtta
     4681 cttcaatatc gtcagttcga atcgacccat cttatccttg gcgatccaga gaaaacaaga
     4741 tgcagtaaaa gacacagcaa agaatattct tgagacaaga gaagctgtta tccatatcct
     4801 tgatgaggac aacgtagaag aagtaaataa aacagcagcc aatctgccta atgaggaaag
     4861 cgaactgtac ctgactgact tgactttaac tgattcagta atcatttcag taccagcatt
     4921 gacagaagca aaagttcgtt ttgaaacatc cttatatgaa catgtcgaga tacggaataa
     4981 aaataaagta acagctgatt tatttttgct agaagtacag aaatatcatt taaatgaaac
     5041 agtctatgat gataagacag gaaggatcga tccaagaaaa ttgaatgctg taagccgttt
     5101 ggcaggaaat gattatgcag caataggaga aattttcacg attgcacgtc ccgaataaaa
     5161 aggagcacta gttatgttaa aaataagcta taaaccatcg acagattcaa aagaaatgaa
     5221 aaaagaatat gaaacagtga atgattttct gcaagggcag tacttagaag ttccgccatt
     5281 gcaagaccat tttgtcgtta ctactgttac tttagatggt aaggaaatag aaatgccaga
     5341 tcaaacgatt agcggattgt ttaattactt taacaaataa atatttattc cgaaaagtga
     5401 aaaaacatat aaaaaaacac ataaaactaa aaaattatca aagaatcctg gtgcaactga
     5461 aagaatttgc aacgggattc tttcaactta tttgttattt ttttctaaaa aatattaaag
     5521 attgttaaat ttcactattt catttcaaat tccattgcct aatcattagg aaaagtctat
     5581 catgaactcg taaatagaat atagtaataa ccttgttagg tgaggctcct atacaaacat
     5641 aggctgttgc ccagaaacgt cgaaagtcgc caatgggtaa aacagaaact gttggattaa
     5701 ggctttttcc aatagctaag aatgttttct ttacgttgta tagtgctaaa actcggacga
     5761 tgagatgatg ctaggtcaga attcttgttc gaaaaggttt ctgacctttt ctttatattt
     5821 ttgtgactga cttatttgat gagacaaact cgctcctcac ctgacaagtg gattgctgtg
     5881 tgaggaggag atgcggtgaa aaaagatcga ttggtggatt ttgggtcctt atttgacttt
     5941 gtccatgatc ggtcttttag aagtatacag tgccagttct tatcgtttat tgcaagccga
     6001 tgaaaataca aaaagtctat tattgcgtca actaattttc atatttttga gttggagcgt
     6061 gatcttctta gctcgttcag tcaaactaca ctatttactt caccctaaaa tagcaggata
     6121 cggtttagcc ttatcgattt tctttttagt attagtaaga atagggatat tcggtgtcac
     6181 tgtcaacggc gcacaacgtt ggatctctct gtttggcatt caattccagc cttctgaact
     6241 ggcaaatctt tttttgattt tttatttaag ctggtttttt cgtgacggaa atagtagccc
     6301 aaaagatcta aaaaaaccat tcctgattac agtaggtata acttttctga ttttatttca
     6361 gccaaagatt gctggagcat tgatgatcct ttcgattgcg tgggtcatat tttgggcagc
     6421 ggcggttcca tttaaaaaag ggatctatct aatcgttact ttttctgcat tgctgattgg
     6481 agcagcaggc ggggtattat atttaggaaa taaaggttgg cttccacaga tgtttaatca
     6541 tgcttatgaa aggatcgcga ctttaagaga ttcttttatc gatagtcatg gtgcgggcta
     6601 tcaaatgacc cattcgtttt atgcactata taatggtggc atatttgggc ggggattagg
     6661 aaatagtatc acaaaaaaag ggtatcttcc agagacagaa acagatttta ttttttcgat
     6721 tattacagaa gagcttgggc taatcggtgc attgtgtgtg cttttcttat tgttctcatt
     6781 atgcatgaga atcttttgtt taagcagtcg ctgtaaaaat cagcaagctg gattgtttct
     6841 tttaggattc ggaactttgc tattcgttca gacgataatg aacgtgggaa gtatcgcggg
     6901 gctgatgcca atgactggtg tgccattgcc atttgttagc tacggaggaa caagttatct
     6961 gatcctttct ttaggaatcg gaatcacgtt gaatatttct tcaaagatac aggccgaaga
     7021 gttggcgctc tatcgacctg aaaaacaata aagagaaact gagaaactct cagtttgaat
     7081 tatcataaag tacaatcttt tatttttcaa gcgagaggtt cttttagaaa gaggaattcg
     7141 caatgaaagt atctcaaaaa atcatattat acccgtggtg ctagttaatt ttttcctaga
     7201 ggaaaatgtc taaacatgtt ggtaaatcaa caaa
//
LOCUS       DAFCHQ010000113         7217 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000113 DAFCHQ010000000
VERSION     DAFCHQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.116"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="LNC71_002592"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="LNC71_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBK6467605.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /locus_tag="LNC71_002593"
     CDS             complement(919..1677)
                     /locus_tag="LNC71_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK6467606.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="LNC71_002594"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="LNC71_002594"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBK6467607.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="LNC71_002595"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="LNC71_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBK6467608.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="LNC71_002596"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="LNC71_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBK6467609.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="LNC71_002597"
     CDS             complement(4433..5290)
                     /locus_tag="LNC71_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBK6467610.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="LNC71_002598"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="LNC71_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBK6467611.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DAFCHQ010000114         7139 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000114 DAFCHQ010000000
VERSION     DAFCHQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.117"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..505
                     /locus_tag="LNC71_002599"
     CDS             <1..505
                     /locus_tag="LNC71_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467612.1"
                     /translation="IQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFS
                     GKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMM
                     FEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRK
                     LVEYGII"
     gene            492..1913
                     /locus_tag="LNC71_002600"
     CDS             492..1913
                     /locus_tag="LNC71_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBK6467613.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1926..2933
                     /locus_tag="LNC71_002601"
     CDS             1926..2933
                     /locus_tag="LNC71_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6467614.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2945..3949
                     /gene="neuB"
                     /locus_tag="LNC71_002602"
     CDS             2945..3949
                     /gene="neuB"
                     /locus_tag="LNC71_002602"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBK6467615.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            3946..5094
                     /gene="neuC"
                     /locus_tag="LNC71_002603"
     CDS             3946..5094
                     /gene="neuC"
                     /locus_tag="LNC71_002603"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBK6467616.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            5067..5744
                     /locus_tag="LNC71_002604"
     CDS             5067..5744
                     /locus_tag="LNC71_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBK6467617.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            5734..6876
                     /locus_tag="LNC71_002605"
     CDS             5734..6876
                     /locus_tag="LNC71_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBK6467618.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            6889..>7139
                     /locus_tag="LNC71_002606"
     CDS             6889..>7139
                     /locus_tag="LNC71_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6467619.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALN"
BASE COUNT         2565 a          920 c         1321 g         2333 t
ORIGIN      
        1 catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta tcttttttga
       61 tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg ctaaagtaaa
      121 tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa
      181 tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga
      241 aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc
      301 tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt taaatgaata
      361 tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt tctgtgaaca
      421 ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag aattaagaaa
      481 gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt aagtccagtt
      541 tctggtatac tatagggaat ttttttatta agggaatttc attcataact attcctattt
      601 ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact tcatttgtat
      661 caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat gcaagttttg
      721 actataaaga tgacataaat gaatttatgt catcggtact ttctctagct acactttcgt
      781 taattgtgtt cttattaata gggaacatat attttttgtt ttcaaatgct ttttttgata
      841 tgaaccaact tgtatttaat ttgatgttat tacaaagttt tgctagtttt gtaattagtt
      901 atatccagtc ttattttacg attaacttta accattttaa atatttgatt ttatctttta
      961 tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta tattttgatg
     1021 ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa
     1081 tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag tggagatacg
     1141 ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt ttagcacaat
     1201 tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata tattcctaca
     1261 ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac gcctgggttc
     1321 catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact
     1381 attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg attgatttag
     1441 caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt ccgataacct
     1501 taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt tatgagaaaa
     1561 aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata ctattaaaca
     1621 tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta tttacgtatt
     1681 ttttactttt tatttttcat tataatatcg ctaaaaaaat tacgaataaa caattatttg
     1741 acacaaaaaa aatatgtatc ataataattt ttgtatcaat tttttctttg atacttcata
     1801 ttttaatcaa tcaaatgttg attcgttacg ttttgacatc attattgata attagtttag
     1861 ttatatattt tttaaagatt aagaatcgaa ttatagatga ctataattca taggaggaga
     1921 tataagtgtt taaaggaaaa acaatattag taactggcgg aactggttcc attggaagtg
     1981 aaattgttcg tcagctttta aaatatgaac caaaagctat cagaatttat agtagaggtg
     2041 aagaaaaaca attttatatg caacaagagc tctcaaatca cactaatttg agatttttaa
     2101 ttggtgatat cagagactct caaaggattg attatgcatg tcgtgatgtg gatattattt
     2161 ttcatgctgc cgcaatgaaa cacgttccag caagtgaata taatcctatg gaagctgtaa
     2221 aaacaaatgt gatgggaact caaaatgtaa tagatgcagc gatcaaaaat aatgtaaaac
     2281 attttgtagg tatcagtaca gataaagtag tgagtcctac taacaccatg ggtgcgacaa
     2341 agttattgag tgaaaaatta ataatagctg ccaatcagta taaaggaaaa acagaaacaa
     2401 tattttcttg tgtaaggttt ggaaatgtca tggggtcaag tggatctgtt attcctcttt
     2461 ttagtaaaca aataaaaaac aataaagata ttactttgac agatgttgat atgacacgat
     2521 ttatgatgtc aataccggat gctgcaagat tagttctgtc tgctgctatg aattcaaaat
     2581 ttggagaaac atatgtctta aaaatgccgg caatcaatat tttaacttta gctcaatcat
     2641 taataaaaat atataatgat acacatgatg aaaaatatag aggtgaaata aaagttgtag
     2701 gtatcaggcc aggagaaaaa ttatatgaag agctaatgac tacagacgaa attgaacgct
     2761 catatgaaaa tgataaaatg tatatcattc caccacacta tcatgctact gacgaactgt
     2821 acaaagatta tgaaaaaatt aaaatcactg catactcttc agatgcaaca gaattaatga
     2881 gtggacagga atttattaga atcgcaaaag agaataatgt cgtgacttgg taaggagttt
     2941 tatgatgaaa tcagaagtat atgtaattgc agaaattggt gtaaaccata atggaagtct
     3001 agatttagct ttagaaagta ttgataaagc taaagaatgt ggagctaacg cggttaaatt
     3061 tcaaacattt aaaacgaata agctagtttc caaaaaggct ccaatggcaa aatatcagaa
     3121 ggataatctt aaagaggaca gttctcaatt tgaaatgcta aaaaaattag aattatctca
     3181 atctgatttt attagaataa aagattattg tatacaaaat aaaattgatt ttttgtctac
     3241 tccttttgat gaagaaagtg cagactttct aaaacaaatt ggtgtacaag gatttaaaat
     3301 tggctcagga gatttaacaa atttaccttt tcttagaaaa atagatatgt atgggttgcc
     3361 agttttatta tctacaggta tggctgatat tcatgaggtc agtgaggctg tagattgttt
     3421 catagatagt ccagttacta ttttacattg tacttcaaat tatccggcta gttctgaaga
     3481 tattaattta ttggcgatta acacattaaa agaaatgttc gggatacccg ttgggtactc
     3541 cgatcattca ttgggatatg atgttggtat ttgtgctgtt gcgatgggtg ctaaggtaat
     3601 tgagaaacat tttaccttgg ataaggaact acctggtcca gatcataaag cttctttaga
     3661 tcctcaggaa tttgcagatt ttgtaaagca tattagaaac actgaaatat ttttaggtga
     3721 tggtgttaag cgagcaatgc cttctgaact tagtacaaaa gaagtatcaa gaaaaagcct
     3781 agtggtcact gagaatttaa atgtgggaga tattcttaca gagaaatctc ttacaataaa
     3841 acgtcctgga aatggaatag ctcctaagtt tttatatcaa tatattggca aaaaagttaa
     3901 aagaccaata agtgcagata gtgttctgct tgaggaagat gttgaatgaa aaaaatactt
     3961 atagtaagtg gaacacgagc tgattatgga atatataaac ctgttgctga gaatttacta
     4021 caagataatt gggatgtatc atttgttgtt tctggaatgc atttatccca taaatatgga
     4081 tttactaaag aattgatctt agctgacgga tttagaatta gtggagaagt tcaaagtttg
     4141 tttttagaga acaataaggg aaacatggct cggtctgtgg cattagagat aaatggtttc
     4201 acaaatatat ttgaacaaac tagccctgat tttgtattat tactagggga tagaggagaa
     4261 atgttggcag ctgcgatagc ttgtttgtat cttggaatca aaacagcaca tattcacgga
     4321 ggcgaagtgt ctggtactgt agatgaatca ataagacatg ccataacgaa aatagcatct
     4381 gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa taaaaatggg ggaagattct
     4441 tggagagttt ttcccgtcgg agctcctaga atagatacta ttttaaataa tagattacct
     4501 agttttagag atataaaaga aaaatatcat ctaaaatttg aaaagaaaaa atacgttttg
     4561 caagtatttc atccagttgt aaccgaacta gaaaatatag aaaaacaaat tttagctttg
     4621 atcaattcaa tggaacaaca atctcaacca attatttgca ttttacctaa tagtgatgct
     4681 ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa aaacggatat tatttatatt
     4741 acgaatttag agccggagga atacctgact atattaaaag agtgcaaatt tatgatcgga
     4801 aattcttctt ctgggataat tgaagctgca tcattcaaaa taccagtaat aaatttagga
     4861 accagacaaa atggacgaga acaatctaaa aatattataa atgcaaatct aaatgaaaat
     4921 gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt ttataaaaag cttgaaaaat
     4981 gttaagaaca tctacggtga cggcaacagt tctagaagaa taacaaaaac attgaatcaa
     5041 atattgaatg atggggaaga tattaagtgg attcaaaaac aaatagctta ttaaaatata
     5101 ctagtgatcc atacattatt attggggctg gcggacacgc aaaagtcatt attgaaacct
     5161 taagattaga aaataagtta atatatggat tttttgatga taagcaaaat aaaatgcaag
     5221 atctgaaata tcttgggaaa ataaacgaat tgaatagctg ggcggatagc aaaaaaaaaa
     5281 tctataaatt tattgtagct attggtgact gtgtagtaag aaaaaagatt ataaaaaaat
     5341 tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc aagtgctata gtggcaaaag
     5401 aaatttcaat tggttatgga actgttattt ttgcgaatgc tgtaattaac ccagatgctg
     5461 taattggaga gcatgctata ataaacacag gtagcattgt tgagcatgat tgtagaatta
     5521 ataactatgt tcatttatct ccaggtgtat gcttagcggg tggagtacat gtaggtgtag
     5581 gaactcaaat aggaattggt tctcaatgta tccagatgaa gaaaattgga tcttggtgta
     5641 tgataggcgc agggagcact attgttaagg atattccctc acattcctta gcttatggca
     5701 atccagctaa aattaagaaa gaaggaatta attttgaatt ctagaatttt attatcaagt
     5761 cctcatatgg gaggaacaga agaaaaatat gttgctaagg cttttaaaga taattggata
     5821 gcgccattag gtgaaaatgt ggataaattt gaagaaagta ttacaaaata tactggttct
     5881 aagggcggag cttctgcgtt aagttcaggt acagctgcca tacatcttgc cttaagactg
     5941 cttaatgttg gaaaaaacga tattgtcttt gctccttcat ttacttttat agctacagta
     6001 aatcctattt tatatcaagg ggctgtacca gtgttcattg actcagaacc taatacatgg
     6061 aatatgagcc ctgttgcact agagagcgca ttagaagaag ctgaaagaaa caaccaatta
     6121 cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg ctgattatga acgaatttta
     6181 gctattgcaa accaatttaa tgttccagtg atagaagatg cagcggaatc gttaggttct
     6241 acttataagg gaatttcgtc aggcttattt gggacaattg ggatctattc atttaacggt
     6301 aataagatca tcacaacctc aggtggagga gctctagtgt ctagtgataa aaaatatgtt
     6361 gagcattcaa gatttttagc aacacaagca agagatcaag caccgtatta tcaacatagc
     6421 gaaataggtt ataattaccg tctaagcaat atttctgccg gtatcggacg tggacaaatg
     6481 gaagtattga atgatagagt agctaaacga agagaaattt tcaatcgata taaggaagct
     6541 ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg atggcatggt gccaaatcgt
     6601 tggttgtcta ctttaacgat caatgattat aattctattg atcaagtagt taacgtaata
     6661 aataaactaa atgaaaaaaa cattgaaaca agagctttgt ggaaaccact acatttgcaa
     6721 ccagtatttc aaggggtaaa aatgtattac catgataata atcttagtgt gtcagaaaag
     6781 ttatttaata gcggcttatg cttaccttct ggaagtaata tgacagatag tgaacaagaa
     6841 tttgttattg atgagtttaa aaaaatattc aagtaggtgt gatattttat gaacatagga
     6901 attattggtt taggttccat ggggaaaaga agacttagac tcattcaaga acactttaat
     6961 gatattttac tatgtggtgt tgatactaat gaggcgagac gaaaggaagt tgaaaaggaa
     7021 tttggaatac caacttataa tagtctaaca gaggcaaaaa atttgtttga aataaattca
     7081 gtttttgttt gtacatcccc tataacccat gaatttatta taaaagaggc tttaaattt
//
LOCUS       DAFCHQ010000115         6895 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000115 DAFCHQ010000000
VERSION     DAFCHQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.118"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..182)
                     /locus_tag="LNC71_002607"
     CDS             complement(<1..182)
                     /locus_tag="LNC71_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBK6467620.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="LNC71_002608"
     CDS             517..951
                     /locus_tag="LNC71_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBK6467621.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="LNC71_002609"
     CDS             974..1294
                     /locus_tag="LNC71_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467622.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="LNC71_002610"
     CDS             1435..1626
                     /locus_tag="LNC71_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467623.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="LNC71_002611"
     CDS             complement(1697..1954)
                     /locus_tag="LNC71_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBK6467624.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="LNC71_002612"
     CDS             2112..2375
                     /locus_tag="LNC71_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467625.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="LNC71_002613"
     CDS             2399..2587
                     /locus_tag="LNC71_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467626.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="LNC71_002614"
     CDS             complement(2670..2855)
                     /locus_tag="LNC71_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467627.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="LNC71_002615"
     CDS             3070..3441
                     /locus_tag="LNC71_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBK6467628.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="LNC71_002616"
     tRNA            complement(3591..3663)
                     /locus_tag="LNC71_002616"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="LNC71_002617"
     CDS             complement(3709..4137)
                     /locus_tag="LNC71_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467629.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="LNC71_002618"
     CDS             complement(4197..4955)
                     /locus_tag="LNC71_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBK6467630.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="LNC71_002619"
     CDS             5250..5447
                     /locus_tag="LNC71_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467631.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="LNC71_002620"
     CDS             5553..6035
                     /locus_tag="LNC71_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467632.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="LNC71_002621"
     CDS             complement(6093..6689)
                     /locus_tag="LNC71_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6467633.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2332 a         1059 c         1355 g         2149 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttg
//
LOCUS       DAFCHQ010000116         6644 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000116 DAFCHQ010000000
VERSION     DAFCHQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.119"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..859)
                     /locus_tag="LNC71_002622"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LNC71_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LNC71_002623"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LNC71_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBK6467634.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LNC71_002624"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LNC71_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="LNC71_002625"
     CDS             complement(2603..3421)
                     /locus_tag="LNC71_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBK6467635.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LNC71_002626"
     CDS             3582..4271
                     /locus_tag="LNC71_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBK6467636.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LNC71_002627"
     CDS             4285..5787
                     /locus_tag="LNC71_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBK6467637.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LNC71_002628"
     CDS             5800..6276
                     /locus_tag="LNC71_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6467638.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LNC71_002629"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LNC71_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAFCHQ010000117         6559 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000117 DAFCHQ010000000
VERSION     DAFCHQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.120"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="LNC71_002630"
     CDS             complement(99..1229)
                     /locus_tag="LNC71_002630"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBK6467639.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="LNC71_002631"
     CDS             complement(1878..3080)
                     /locus_tag="LNC71_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6467640.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="LNC71_002632"
     CDS             complement(3140..3343)
                     /locus_tag="LNC71_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBK6467641.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="LNC71_002633"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="LNC71_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="LNC71_002634"
     CDS             4880..5692
                     /locus_tag="LNC71_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467642.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1986 a         1329 c         1026 g         2218 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtt
//
LOCUS       DAFCHQ010000118         6529 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000118 DAFCHQ010000000
VERSION     DAFCHQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.121"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..774
                     /locus_tag="LNC71_002635"
     CDS             37..774
                     /locus_tag="LNC71_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBK6467643.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            788..1603
                     /locus_tag="LNC71_002636"
     CDS             788..1603
                     /locus_tag="LNC71_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBK6467644.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1616..2263
                     /locus_tag="LNC71_002637"
     CDS             1616..2263
                     /locus_tag="LNC71_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6467645.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2279..2935
                     /locus_tag="LNC71_002638"
     CDS             2279..2935
                     /locus_tag="LNC71_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBK6467646.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3097..3918
                     /gene="racE"
                     /locus_tag="LNC71_002639"
     CDS             3097..3918
                     /gene="racE"
                     /locus_tag="LNC71_002639"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBK6467647.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3921..5276
                     /gene="rph"
                     /locus_tag="LNC71_002640"
     CDS             3921..5276
                     /gene="rph"
                     /locus_tag="LNC71_002640"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBK6467648.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5277..5795
                     /locus_tag="LNC71_002641"
     CDS             5277..5795
                     /locus_tag="LNC71_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBK6467649.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5902..6402
                     /locus_tag="LNC71_002642"
     CDS             5902..6402
                     /locus_tag="LNC71_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBK6467650.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2151 a         1097 c         1443 g         1838 t
ORIGIN      
        1 agaaatctta ctaaataata atgggagtgt aattctatgg caatgattaa atttgatcac
       61 gtggataaat attatggcaa gttccacgca ttaaaaaata tcaatctcga attcgagaaa
      121 ggagaagttg tcgttgtcat tggtccttct ggttcaggaa agagcacgat gcttcgctgt
      181 atcaatggat tagagacgat ctcttcaggt aagctgctga ttaatgatac tgacttgcat
      241 gataaaaaga caaaactgac agaagtccga aaaaacatcg gcatggtttt ccaacatttc
      301 aatttgtacc caaataaaac tgttctggaa aacatcaaat tagcaccaat caaagtgttg
      361 aagcaagacc aggcaactgc agtcaaaaac gcggagaaat ttttgaaaac tgtcaatatg
      421 ttagataaga aagattctta tccttccatg ttatccgggg gacaacagca acgggtggca
      481 atcgccagag gtttagcgat gaatcctgag atgctgctgt ttgatgaacc tacttctgct
      541 ttggatccgg aaatgattgg tgatgtatta gacgttatga aaaaattggc tcatgacggt
      601 atgtcaatga ttgtcgtgac acatgaaatg ggctttgcca aagaagtggc ggatcgtgtt
      661 atcttcatgg ctgatggaga agtattggaa gacagcagaa atgtgagaga ctttttcgaa
      721 aatccaaatg aagtccgagc acagcaattt atcagtaaag ttatcaatca ttgataggag
      781 gaaaaagatg cgcaggttaa aaaaaatacg cctgattttc cttttaggca ttgcttttct
      841 tgcactagcc ggatgtaaag gaaatgggtt ggcaaatgaa gatattttag agcgaagcaa
      901 atctaccaat gaaatcatct ggggtgtcaa gtatgatact cgattatttg gaatgatgga
      961 tattgagagc agaactgtac aaggatttga tgtagatatt gcaaaagcaa tcactaaaaa
     1021 gatccttggt gacaatggaa aaacagaatt tgttgaagtc acttctaaaa ctcggattcc
     1081 attattgaaa aacggaaata tcgatgcaat tattgcaacg atgacgatca cagatgaacg
     1141 aaaaaaacag gttgactttt cagatgtcta tttcgatgct ggccaagctt tgttggtaaa
     1201 gaaaggaagc caaatcaaaa gtgtagatga tttgaatgct tctacaacgg ttctagcagt
     1261 caaaggttct acatcggctg cgaatatccg acagcatgca ccagatgcaa aaattttaga
     1321 attagaaaac tatgcagaag catttactgc tttacaatct ggtcagggag acgcgatgac
     1381 tacggataat gcgattttgt tagggattgc agatgaaaat ccagagtatg agttagttgg
     1441 tggaacgttt actaatgagc cttacgggat tgcgatcaac aaagggcaag aaaacttctt
     1501 gaaagctgtt aaccaagcgc tagaggaaat gcatgcagat ggcacatacg ataaaatcta
     1561 tcaaaaatgg tttccaaatg aaactgaagg aaaagtagag taagggggag aggcaatgag
     1621 cgaattgata caaacttatg gaccaatctt tgtcgacggg ttcaagatca cattgttttc
     1681 gagtgtctta gcacttgtgt ttagtttgat tatcggaacg ttaatggcaa tcttccaatt
     1741 atcaaagaat aaagtagtga gcgccttagc aaaggcatat gtagaatttt ttagaaacat
     1801 tccattatta atcatcgtga tgttttttta tgttgtcgta ccgatttatt ggtttaatct
     1861 agatggattc caagcaggta cgattggatt aacgatctat acatctgctt ttattgctga
     1921 aacagtacgt tcagggattc aaaccgtacc aaaaggtcaa acagaagcag gactatcttc
     1981 tggttttacc tattcagaaa tcatgcgtta tattgtttta ccgcaagcat tcaaaatcgt
     2041 tatcccgcca ttaggcaacc aatttatcaa cctcgtaaaa aactcatcta ttttagcaat
     2101 cgtagctggt ttagatttga tgtatcaagg agacttgatc gctagtgaaa cattcaatac
     2161 cttcgatacg tatatcatcg ttgggttatt ttacttagtt atcacgctac cgttatctta
     2221 cttaatggtt tatcttgaga aaaaatgggc ggttcgttca tagaaggagg ggaaatacat
     2281 ggattttagc ggcgcttttt catggatgaa tatccgtttt ttattggaag gattaaaggt
     2341 tactgttgaa gtttcattgt tttcgatcat ttttagtttc ttgatcggtg gtctgacagg
     2401 tgtgattcgt tttgcgaata tccctttctt ttccaaaatc cttggtgtag tgattgatat
     2461 tatacggaat cttccattat tattgatcat tttcttcact tactttgcct tacctcaaat
     2521 cggcattcag atgaacatct tctggtccgc tgtcgcagca ttaacgattt ttgagtcagc
     2581 tatgttgtcg gaaattttcc gagctggact caatgctgtt ccaaaaggac agatggaagc
     2641 aggattatca acgggactga cttatgtaga atcgatgcga acgatcgtgt taccgcaagc
     2701 atttaaatca atgatcccgg caattgtgag ccaattgatt tcactgatca aagatacttc
     2761 attagcagta atcatttctt tacctgaact ttctcatcag gcacgtatta tttatggaca
     2821 gaacaccaat tatgtgcttc cgatgttcat catcatgaca cttatgtact tcgtggtatg
     2881 ttacgcgctt tctctgcttt ccggttactt ggaaaagaga cggtacagtt attaatagat
     2941 gattctttta gcggttgtgg caaactaaag tcacaaccgc ttttcctctt tccattcgaa
     3001 aaaaagagat ttcgattgtt ttaagcaagt aagagcactt gaaaattcac gctaaaaagt
     3061 acacaatagg gaggatagaa tttattgaaa aaaaggatga tacgattgac agataatcgc
     3121 cctatcggat ttattgattc aggtgtcggc ggcttgactg tagtaaaaga agccctgaaa
     3181 caattaccga atgaaaatat tttatttgta ggagacacag cacgctgccc atatggccct
     3241 agacccgcgg aacaggtaat ccagtatact tgggaaatga cggattatct ggtggagcaa
     3301 ggaatcaaga tgctggtgat cgcctgcaat accgcaactg cggtggcttt agaagaaatc
     3361 aaagctgctc tttctattcc agtcatcggt gtgatccttc ccggtactag agcggcagta
     3421 aaaaaaacac aaaataaaca agttggcatt atcggtacga ttggtacggt aaaaagtcaa
     3481 gcttatgaaa aagcactgaa agagaaagta ccagaattga ctgtgacaag tcttgcttgt
     3541 cccaaatttg tttcagttgt cgaaagtaat gaataccatt catcggtggc gaaaaaaatt
     3601 gtggcagaaa cattagctcc tttaaccact aaaaaaatcg atacattgat tttgggatgc
     3661 acccattatc cattattacg ccccatcatt caaaatgtaa tgggagaaaa tgttcaactg
     3721 atcgattctg gagcagaaac agtaggtgaa gtatctatgc tgttagatta tttcaatttg
     3781 agcaattcac cgcaaaatgg tcggacatta tgccagtttt atacaactgg ctctgccaaa
     3841 cttttcgagg aaatagctga agactggctt ggaatcggac acttaaatgt agaacatatc
     3901 gaattgggag gaaaataagg atgcgtcatg acgggagaaa agcccaagag cttcgcaaac
     3961 tggagcttca aacaaatgtt ttcaaatatc cagaaggatc ggtagtgatc cgttttggtg
     4021 atacaacagt tatttgttct gcaacaatag aaaattctgt tccccctttt ttgagagaaa
     4081 caggaacagg atgggtgaca gcagaatata gtatgctgcc tcgagcaacg agtacgagaa
     4141 atcgtagaga aagcagcaaa gggaaacttt ctggtagaac aatggaaatc cagcgtctta
     4201 ttggtcgttc cttgcgtgca gtagtagact tggaaaaatt aggagaacgt agtatcatcg
     4261 tagactgcga tgtgatccaa gcggatggcg gtactagaac agcaagtatc actggggcat
     4321 ttgtggcatt gaaattagcc attgaaaaac ttttaagaga aaaagaattg tcagaagatc
     4381 caataaaaga acatcttgct gcagttagcg taggaatctt accagatggg acttgcgtca
     4441 cagatttgga ttaccaagaa gattccgcag cacttgtaga catgaatctt gtaatgacag
     4501 aatctggaaa attcgtggag attcaaggaa ctggagaaga agcaacgttt gatggtgaac
     4561 agttgaatga aatgctcttt tttggaaaaa acgcgattga agatttgatc aaagagcaaa
     4621 aacatgcttt gctaacagag tttgctcaaa atgatgagcg gatagaagaa acaaaaacaa
     4681 tcatcattgc gacaagaaat ccaggaaaag cagaagagtt tcgaaatatg ttcaaagaag
     4741 cgggatatca tgtaaaaaca ttacttgact atcctgaatt gccagatgtg gaagaaactg
     4801 gcagcacatt tgaagaaaat gcccgattaa aagcagaaac gattgcacaa ttgcttgatc
     4861 agccagtctt agcagatgat tcaggtttga aagtggatgc tttaggcggt atgccaggaa
     4921 tctattctgc acgttttgca ggggaacaaa aaagtgatgc ggggaataac gccaaattat
     4981 tatatgaatt gacagatgtg ccggatgaaa aaagaacagc acaattccat tgtacgttag
     5041 tattcgcggc acccaaaaaa gacagccttg tagtagaagc ggaatggccc ggtcgagtag
     5101 cccgtatacc atctggcgaa aatggctttg ggtatgatcc tttatttatc ccagaaggta
     5161 agaaacaaac agcagcagaa ttatcgagcg aagaaaaaaa caagatcagt catcgggctc
     5221 aagcaatgaa aaaattgagt gccgagtgga aacaatggct ggaaggagag agatagatgc
     5281 ggtatttagt agtaagtgat aatcatggag acagagatat cctcgttgac ttagtagatc
     5341 gttatcgttc tgaagtggat atctttttcc attgtggtga ttcggaatta gaagcttctg
     5401 acccattatg ggatacattc caagtggttc aaggtaattg tgattatgga ccaggattcg
     5461 aacagaaaaa agtgatccaa actggtcagg atacagtatt catgactcac ggtcatttat
     5521 cgaatgtccg atttggtttg acacagttag caatcgaagc tcgagctgca actgccgata
     5581 tggtgttttt tggtcatacc catcaaatag gctgtgagat ggatcagaaa atcctgtttt
     5641 tgaatcctgg tagcatttct cagcctagag gcccgatcca aatcccatca tatgctctta
     5701 tcgaaagttt agatgaccaa atttctgttc aatactataa ccgtgcccat caggcagttg
     5761 aagagttggc cttttctttt tcgagagagc gctgaataaa aaaactctaa tttttaataa
     5821 aaataagctt tattttatga attttgcatc cggtttcggt aaaatagtag tgttgaatga
     5881 aatagaaatg gaggccatca gatgattgga cctatagtaa gagaaatgct gctggaaaat
     5941 caggaaacgt ttttgattcc tgcagaaaat gtggcaaatg ttatgtatca tcatccattg
     6001 tcccatggtt tacttgtgtt atcgaaggtc ggttattcca aaattcctgt attaggaaaa
     6061 gatgatcact ttgtaggact agtaagttta tcagatgtgg tcaataaaat gattgacttg
     6121 caaacaatca gcatggaccc gttagaagga ctaacggttg ctgatgtgac agaaacagat
     6181 gttccgacaa ttgatgaaaa ttgggaacta gaagaagtac tccatttgtt agtcgattcg
     6241 ccttttttac cggttgtcac agaagatcgg attttcaaag gaatcattac aagaaaagag
     6301 ctgcttaaag ccgtaaactt catggtgcat gaattagaac gtcgtaatat attgtctccg
     6361 aaaatcgaag aattaaaaga acaaatcgat atcgtgagct aaagaacgag aaactaaaag
     6421 ataatcaaaa aataagtaga aaaaacagcg taccagtttt gactttatct tgaaaaagag
     6481 gctgggacaa aagcgtttaa ctccaagaaa taagaaggaa ttcacgaaa
//
LOCUS       DAFCHQ010000119         6446 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000119 DAFCHQ010000000
VERSION     DAFCHQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.122"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(489..863)
                     /locus_tag="LNC71_002643"
     CDS             complement(489..863)
                     /locus_tag="LNC71_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBK6467651.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(886..2286)
                     /locus_tag="LNC71_002644"
     CDS             complement(886..2286)
                     /locus_tag="LNC71_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBK6467652.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2290..2700)
                     /locus_tag="LNC71_002645"
     CDS             complement(2290..2700)
                     /locus_tag="LNC71_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBK6467653.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2718..3209)
                     /locus_tag="LNC71_002646"
     CDS             complement(2718..3209)
                     /locus_tag="LNC71_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6467654.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3219..4010)
                     /locus_tag="LNC71_002647"
     CDS             complement(3219..4010)
                     /locus_tag="LNC71_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6467655.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(4003..4776)
                     /locus_tag="LNC71_002648"
     CDS             complement(4003..4776)
                     /locus_tag="LNC71_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6467656.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4932..>6446)
                     /locus_tag="LNC71_002649"
     CDS             complement(4932..>6446)
                     /locus_tag="LNC71_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6467657.1"
                     /translation="DLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSS
                     EYNYLESDEFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFL
                     LSKFRKFRMKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIV
                     MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLV
                     DMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVK
                     VQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDT
                     MIFKNTICVLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLF
                     TIEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIG
                     DGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         2075 a         1273 c          833 g         2265 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaaacta
      421 ttgaaatgct gataccaaaa aatgaagtac ttttcgttca taccaactta gagcacttcg
      481 ttatctattt atttcttcat ttggcttatc tctataatct catgatttgg tccgtagaaa
      541 ttaaagtaac ggattccatt ttcccagaac ggtaattcca taatattttt atctaataat
      601 tcatatccta aatcttgaat tgaattgaat acctcatcta tgtcatccgt atctaatgct
      661 agatgatcaa tggctcctct acactcagta gttttatctt cccatatttc tattatcgtt
      721 ccattcaact cgaaaaagcc gactttactt cccacattca tttcttcatt aatcagacta
      781 aaccctaagg attcataaaa gatttttgaa gctccaaaat cttttgttgg aattccaata
      841 tgttgtaaac catttaattt cacttacatc atcccttcaa ttgttttaaa atccacattc
      901 ataatttatt gaatagtcta gtttatctac cttgattgct ccctccgtaa cagtcatact
      961 taatgctgtt ccgactttat catacattct agtacttaaa gcaacattat caatatataa
     1021 aacagctatc gtatcatcaa caattaactt tagttcatat tctttgccta tttccaaaaa
     1081 tagtggtcgt tgcagcccct tatcaaacat ttgaggccaa ggatagttcg gagtttttcc
     1141 gaaagtaagc tgattattaa tcaatgaaat acgaaattcg tatccttctt gagtatcact
     1201 atctacaaat aattttaaag caaactcctt tgttaacgat gtaatagtca aagttgtttc
     1261 aaatagaaaa taatcttcga ttttaggaac aattactctt tctttttttc caaattcttt
     1321 ctctaactta aaacttgaaa atggagtgat ttctttaaaa ttctcaatta ttttttcagg
     1381 cagtttaaca ccaagagttt tgtctgtctt ttgaataatt tgatgtggaa caagtgcacc
     1441 gccccaatcc cagttatttt gatcgttctc cccttctttt gttgccaccc agccagttag
     1501 aaaacggcca gtttcattag ttccgactgt tcttgctgca taataggctt ttccgtcgaa
     1561 agcttcgtct actggtgatt tccaatctga aaataatcct gctccgactc gatatttagt
     1621 ctttttatct tcacaatact ctgaaaaaag taaataccac gttccatcaa tttcaaatat
     1681 atcaggcatt tcaatcatgt taaattcatt attagcccag tagtctcctt tgaaattcca
     1741 atcttttaaa tcttcggatt caaaatagac caatctcccc gtcggctttt tcttacttgc
     1801 tggttttcta gctcctagta tcaaaacgta ttttccatga tcttgatcaa aaattgcaaa
     1861 aggatcgcgc caatcattct tatcatatcc ttcttgagga acaagttttg ctgcttcgcc
     1921 atcctttttc caatgaatta aatctttaga acttgcaatc attaacactt ctgaagtttt
     1981 tccatccaac ttagctttgc gattatgtcc agtatacaaa gctaaatact cattgattcc
     2041 ttggacaata cttccagcgt aaatgtattg atcaatgtca tcgttgccac cacgatgaat
     2101 tacttccccg tattcttcaa atttcacaaa atcagtggtt gtaactagtg accacccaaa
     2161 aggatcagtt aaaggtttag gatttctcgt atctcgttga tgaaatagat aaaaagttcc
     2221 atctttataa aaaggcatgc aatccccaaa ccaaaaatct ttaggcttgt aaaaaatttt
     2281 ttgcatttat cactcatcct cttcgttagt atgtctttgc atatctgcaa gacacgcaat
     2341 tgtattcata tttcttagat tttcgatgat atcatcgctt ttttttcctt gtaatctcaa
     2401 attgacagcc tcaatcaaaa ttcctaaatt catgccacca agaagttgaa acccattttg
     2461 aaatacttca cttcttttag caaattcgtt gaaaggtgtt ccaccaatca agtcagcaaa
     2521 taccataact tcttgatttt ttacttgaga aactattttt tcaatgtttt cacggaattc
     2581 ttcaatgcct gcatctccaa gtgttaccac atgacaattt aaattatcgc caaaaaacat
     2641 ttttaatgta tctacaaatc caatggcaat atttccatga gatgtaatta taaactccat
     2701 ccactcatcc tctgttctta ataaataaaa tcctttaaaa attcttttga atctctagga
     2761 gtttgtatca ctgtcacttc aactccatca ctaattagtt ctttaaaagc ctctctctct
     2821 tcttcagtaa tatttatcgt cggacggatg cgttctgttc cattccttct tgataaattt
     2881 cctacgttta cacttgttat tggtaaaccg ttatcaatca aaaatttaac atcgattgga
     2941 gaaactgcaa caataagaac tttttgtcct tcatatttac cagaaagaat atttttaact
     3001 gctacttccc ttgtaattat tgaagttcta acattaggtg gtgcagcgag gcgaactaca
     3061 gcttttttta cgtcatcatt ggctacttca ttattaataa ccataattct gttagcactc
     3121 aattgatttg tccattgtgt tgctacttgt ccatgaatca ttcgatcatc aatacgaaca
     3181 tgtataatac ctttcatttt ttcatccata ttcaacacct acttcaaaat atttaacgct
     3241 cttaacgcaa tagaaactac taagattaat agaattacat aaactggttt aacctttcgt
     3301 cctaacaaat aatacacaat tgctactaca catgcaggta ataaaccagg cataacttga
     3361 tcaaagattt catttccggt cattgttaag tcgccttgtt taaattcaaa gccaacattc
     3421 acttttacaa ctgtggcaat gagtgcaccg accaccatta accctaaaat tgatactgca
     3481 tcagtaatag actttaattt tgtttcaatt tgagttagta ggctcgcacc cgattctttc
     3541 ccagcaaggt ataaaggata agcagtaatc cttaataaaa ttgatgccag tatccacaaa
     3601 ataattccaa caaaactacc ttccaatgcc atattagctg caattgctcc aaaaatagtc
     3661 catggaataa ctacaaatac actatctcca attcccgcta aaggacccat tagacctgtt
     3721 ttcagagcac ttactgcttc caaagactta tttttttctt ctttttcaat tccaacattc
     3781 ataccaataa tgtaaggcgc tgtatacggc gtagtgttaa aaaattgtag gtgagctaaa
     3841 acggattgtt tcattccttc aggatcatct ttatatgtgt ccttgataaa aggaagcata
     3901 gaatacgcat aacccaaccc ttccattttt tcataattta atgaagttgc accattgaga
     3961 atccaccgga aaattacttg tttgtcgatt tttgtcagtt tcttattcat cttcgtcaaa
     4021 acctcctgtt gcagctggtg ctacttgagt attatttgat ctatttttat atacgattag
     4081 tgcgattgca gcgccaagta acgaaatccc taaaattggt atttggaaat aaactgccaa
     4141 aacgaaacca actaacaaat aggcaaagta atcttttgta ggtaaatatt taagtagcat
     4201 tcctatccca acagcaggta atagtccccc tgctaccttt aaaccaccgg taacccattc
     4261 aggaatataa ttgattgcac ttcccactaa cgttgggcct aatatcacag ttaacaaaac
     4321 aggaattcca gatacaaagc ttgtattaat tgatgccaaa tattgcatcc actctaattt
     4381 ttgataatct tctttttgtg cgaatttttc tgccttttgt tgaaaaaaaa tgtttgttga
     4441 ccactttaga acatctagtt gtacaattaa cattgctacc ggtattgcta aagttattcc
     4501 tatagaagca tcctggttag ttgagattgc taaataagta ccgatcaatg ctgctgtttg
     4561 ataatccggt actgatgcac caccaaaact agtaatacct agtgtcatta attgcaaagt
     4621 cccaccgata taaagtccag tttgaatatc tcccataata agtccggtca agaaacctgc
     4681 aacgacaggt tgggaggttc cgattttggg cccttcctta tcccagttaa tgaaaaatcc
     4741 atacaacaca actaaaaatg tttgccaaaa cgccattttg ttctctcctt tcttcttata
     4801 cttttatata agcaattact gtgccaacta acattatcca ataaagcggg aaaatacagt
     4861 aaatattaat taaatgagcg tctaaatata ctttggacac cctttcattt ttttagacgc
     4921 tagtatgcta tttagaaaat agtctgctca aaaattgtat agattaactc atactctcct
     4981 ttgggtattt caatacagta aatatctttg atttctgaaa aaaaatcttt tgatacattt
     5041 aaaaaagtat caaatagttg cgctccctct ttcaaatgtt catttgttat tccatcacca
     5101 atcataattc gttcaatcat tccagaaaga tgtagaaata gattaattct cagaaaactt
     5161 gggattttga catcgtaata tttttcatac aaatatagta ctttttctat ctcattgaat
     5221 acttttttgg aatctaaaaa gcgaagctta gtactagcgc cttcaatagt aaagagttta
     5281 actaattcat ttaagcagaa ctcacagcta tccgagtcca tatacttatt taatttttct
     5341 aaggtttgat tcccattaat tagattttct aaatttaaac aatcgattcc ttgaatgtaa
     5401 aattcagtgg ttgaaagaac acaaattgta ttcttaaaaa tcatagtatc tttttccttt
     5461 gctattttct ttaatgcgtt aaaatcaaaa atcaaaatct ccacttgatt atcgagatac
     5521 cgttttaaaa tatccttaac tttttcagaa atcccctcac cagagagaca agatactatt
     5581 acatttttct tttgtgataa tcctttataa tactgaactt taacttgaaa cgaatcaaaa
     5641 tccatctggt aaaaatgact aattggcttt ttttggcaaa cttgaatccc acattcaatc
     5701 gctaaagctg tagaaacatt gtttaaaatt accaaatcac ctattacatt ggattcaatt
     5761 ttttcataca tttgttccaa tgatcccata tcaactagca gaatgagtcc attcgtcgta
     5821 tctatacttt tacaaaaatt gttgattagg ttaattaagt cactagtctc accttcaatt
     5881 ggcatatcga aagcctcata aacatatgta ttcagcaact tatttgtttc ccttgcgatc
     5941 gcagaagctg ttattttacc gtgcataact atcagacaat gggtttctgg tattttcaca
     6001 tagaacttct ttacaagaaa aagtatccat ttcaacaaaa gttcttgttt actttcttcg
     6061 acttctaaac tactccaaat ctcatttgcc aataattttt gtttatcatt tttcatacga
     6121 aattttctga acttagacag caaaaaattt cttgatgttt cttcaattac atcaataaac
     6181 attataaata gactgatatc cgccaagaat tcatctaata aacatagacc tgtcgaatca
     6241 ataattggtg atattttatt aacaacatag tttcttaatg ctataaattc gtcactttct
     6301 aaataattat actcagaaga acctgcacac tctttaagta ttctatctct taattgactc
     6361 atgctttctt caattgaaat gtgattttga aaaagttcaa tcaaatcaat ttcagaaagt
     6421 gatttaagaa acattttttt taaatc
//
LOCUS       DAFCHQ010000120         6173 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000120 DAFCHQ010000000
VERSION     DAFCHQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.123"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            725..811
                     /locus_tag="LNC71_002650"
     CDS             725..811
                     /locus_tag="LNC71_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK6467658.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="LNC71_002651"
     CDS             complement(932..1621)
                     /locus_tag="LNC71_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467659.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="LNC71_002652"
     CDS             complement(1628..1906)
                     /locus_tag="LNC71_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467660.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="LNC71_002653"
     CDS             complement(1866..2780)
                     /locus_tag="LNC71_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBK6467661.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="LNC71_002654"
     CDS             complement(2762..3142)
                     /locus_tag="LNC71_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBK6467662.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="LNC71_002655"
     CDS             complement(3592..3879)
                     /locus_tag="LNC71_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6467663.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="LNC71_002656"
     CDS             complement(3899..4123)
                     /locus_tag="LNC71_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBK6467664.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="LNC71_002657"
     CDS             complement(4578..4748)
                     /locus_tag="LNC71_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467665.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="LNC71_002658"
     CDS             complement(4763..5296)
                     /locus_tag="LNC71_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBK6467666.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="LNC71_002659"
     CDS             complement(5317..6159)
                     /locus_tag="LNC71_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6467667.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAFCHQ010000121         5925 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000121 DAFCHQ010000000
VERSION     DAFCHQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.124"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(76..1170)
                     /locus_tag="LNC71_002660"
     CDS             complement(76..1170)
                     /locus_tag="LNC71_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6467668.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="LNC71_002661"
     CDS             1359..2120
                     /locus_tag="LNC71_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6467669.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="LNC71_002662"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="LNC71_002662"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBK6467670.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="LNC71_002663"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="LNC71_002663"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBK6467671.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="LNC71_002664"
     CDS             complement(3760..4773)
                     /locus_tag="LNC71_002664"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBK6467672.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="LNC71_002665"
     CDS             4938..5858
                     /locus_tag="LNC71_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBK6467673.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DAFCHQ010000122         5771 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000122 DAFCHQ010000000
VERSION     DAFCHQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.126"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1016..1306)
                     /locus_tag="LNC71_002666"
     CDS             complement(1016..1306)
                     /locus_tag="LNC71_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467674.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1708..1944
                     /locus_tag="LNC71_002667"
     CDS             1708..1944
                     /locus_tag="LNC71_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467675.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="LNC71_002668"
     CDS             1951..3903
                     /locus_tag="LNC71_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBK6467676.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="LNC71_002669"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="LNC71_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBK6467677.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4799..5491)
                     /locus_tag="LNC71_002670"
     CDS             complement(4799..5491)
                     /locus_tag="LNC71_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK6467678.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1895 a          997 c          917 g         1962 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagttgaa
     4561 taagcaattt tttctataag ttatattatc ataataccac aaaatcacaa attttcattc
     4621 aattatcatt ttcaattgca ttttataaat cattttgtat gtaatttggt atgtaagtta
     4681 aaatgaaaaa agtagtccat tttgaagtac attttgtcat tttgtactcc aaaactgaac
     4741 tacttagtat agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt
     4801 atttacgcaa atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat
     4861 cttttgcttt ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt
     4921 cgtctaattt cttttgtgta tctgttaact gagtttctac atttgttttt tcttgtgtca
     4981 gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt
     5041 gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt
     5101 ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct
     5161 tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt
     5221 ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct
     5281 gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta tctgtcaatg
     5341 tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca
     5401 aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga
     5461 caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta
     5521 catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta catatgtaat
     5581 tttaatataa atactatcaa atactcattt aatcctcctt ttttatctat ttaaaaaatc
     5641 ttttttatag agaaaaatgc aggttaatac tttcttaaat aaatatttat ggtgaacaaa
     5701 aacaaaaaag caagttttat taaacgtaac ccttttcatt tcatagatta ctgtatttat
     5761 tacaaaaaac g
//
LOCUS       DAFCHQ010000123         5689 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000123 DAFCHQ010000000
VERSION     DAFCHQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.127"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(488..1297)
                     /locus_tag="LNC71_002671"
     CDS             complement(488..1297)
                     /locus_tag="LNC71_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBK6467679.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="LNC71_002672"
     CDS             complement(1297..2043)
                     /locus_tag="LNC71_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBK6467680.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="LNC71_002673"
     CDS             complement(2061..2528)
                     /locus_tag="LNC71_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBK6467681.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="LNC71_002674"
     CDS             complement(2528..2938)
                     /locus_tag="LNC71_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBK6467682.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="LNC71_002675"
     CDS             complement(2949..3962)
                     /locus_tag="LNC71_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6467683.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="LNC71_002676"
     CDS             4179..4913
                     /locus_tag="LNC71_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6467684.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="LNC71_002677"
     CDS             4897..5607
                     /locus_tag="LNC71_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6467685.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAFCHQ010000124         5481 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000124 DAFCHQ010000000
VERSION     DAFCHQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.128"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="LNC71_002678"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="LNC71_002678"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="LNC71_002679"
     CDS             complement(580..1353)
                     /locus_tag="LNC71_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBK6467686.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="LNC71_002680"
     CDS             1497..1847
                     /locus_tag="LNC71_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBK6467687.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="LNC71_002681"
     CDS             1828..2544
                     /locus_tag="LNC71_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6467688.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="LNC71_002682"
     CDS             2544..3992
                     /locus_tag="LNC71_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6467689.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="LNC71_002683"
     CDS             complement(4062..4571)
                     /locus_tag="LNC71_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467690.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="LNC71_002684"
     CDS             complement(4561..5286)
                     /locus_tag="LNC71_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467691.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAFCHQ010000125         5311 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000125 DAFCHQ010000000
VERSION     DAFCHQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.129"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="LNC71_002685"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="LNC71_002685"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBK6467692.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="LNC71_002686"
     CDS             complement(1297..2133)
                     /locus_tag="LNC71_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467693.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="LNC71_002687"
     CDS             complement(2317..3534)
                     /locus_tag="LNC71_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6467694.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="LNC71_002688"
     CDS             complement(3683..3862)
                     /locus_tag="LNC71_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467695.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="LNC71_002689"
     tRNA            3917..4000
                     /locus_tag="LNC71_002689"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="LNC71_002690"
     CDS             complement(4240..4818)
                     /locus_tag="LNC71_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467696.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4824..5207)
                     /locus_tag="LNC71_002691"
                     /pseudo
     CDS             complement(4824..5207)
                     /locus_tag="LNC71_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DAFCHQ010000126         5189 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000126 DAFCHQ010000000
VERSION     DAFCHQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.130"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..748
                     /locus_tag="LNC71_002692"
     CDS             137..748
                     /locus_tag="LNC71_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6467697.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="LNC71_002693"
     CDS             821..1750
                     /locus_tag="LNC71_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBK6467698.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="LNC71_002694"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="LNC71_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="LNC71_002695"
     CDS             complement(2693..3871)
                     /locus_tag="LNC71_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK6467699.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3969..4067
                     /locus_tag="LNC71_002696"
     CDS             3969..4067
                     /locus_tag="LNC71_002696"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6467700.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="LNC71_002697"
     CDS             4098..4499
                     /locus_tag="LNC71_002697"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6467701.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5189)
                     /locus_tag="LNC71_002698"
     CDS             complement(4706..>5189)
                     /locus_tag="LNC71_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBK6467702.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAFCHQ010000127         5182 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000127 DAFCHQ010000000
VERSION     DAFCHQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.131"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..977
                     /locus_tag="LNC71_002699"
     CDS             <1..977
                     /locus_tag="LNC71_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBK6467703.1"
                     /translation="TIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGG
                     VFWNYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIP
                     ELFIIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQ
                     GLLEYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEF
                     YKSFGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAI
                     MSVFFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            974..1693
                     /locus_tag="LNC71_002700"
     CDS             974..1693
                     /locus_tag="LNC71_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK6467704.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1708..2793
                     /locus_tag="LNC71_002701"
     CDS             1708..2793
                     /locus_tag="LNC71_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6467705.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2929..4461
                     /locus_tag="LNC71_002702"
     CDS             2929..4461
                     /locus_tag="LNC71_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBK6467706.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            4639..4860
                     /locus_tag="LNC71_002703"
     CDS             4639..4860
                     /locus_tag="LNC71_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBK6467707.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1798 a          553 c          997 g         1834 t
ORIGIN      
        1 taacaattaa agattactat aaagtattcg ctataattga aaaattattc tggatttcac
       61 tgccactttg tttaattgaa agattcatgg tcacctatcc aactggaaca attgtaggag
      121 atatgatagg tggagttttt tggaattatt caggttgtaa tatgccattg aatttaattt
      181 tgtgtgtgac agttactgtt gttacaatcg gatatttcaa gggagaaaaa aatttcatcc
      241 attttatttt atgtgtttgt gcagcacttt ttatgtctgc attagctgaa ttgaaagttt
      301 ttattcctga attatttata atatttgttg caatagtatt cactttaaaa gttgatttta
      361 aagtagtgat caagatatta tcatgcgtaa tacttgtgtc gatagtatta tcctttttta
      421 taacaatatt tattacattg aacgataatg gacaaagcta tgctgataat ttcacaattc
      481 aaggtctttt agaatatgcg tcaagagatt ctggatatga tggtgttggt gatttaaatc
      541 gaataactgg tatctctaga gtagtagatg aaatatttaa taataatttt agattagtcc
      601 taatgggagt aggcattggc aatgcagagt ttaccaattt ttttgtcagt gagttttata
      661 aatcgtttgg taatcttcat tatcagtatt ttcatcatat ttggttattt atagaagttg
      721 gaatattagg agtttcgata ttcctatttt ttataataaa gacatttttc aatgcaaaaa
      781 gatcttttat gaaggataaa gatgcaagtc ttgttaaagt tattgcaatt atgtcggtat
      841 tcttttttgt gtataacaca acacttaggg gtgaaacaac ggggccactg atttatatga
      901 ttttagccat gcctgaagtt gtgaaaagga aagagcatta ttatagtgaa aaaaatacaa
      961 aaggagagat attatgattc ctaagaagat acattactgt tggttcgggg gaaatccact
     1021 tccgaaaaaa gatagaaagt gcatagaaag ctggaaaaag tattgccctg attatgagat
     1081 tattgaatgg aatgaatcaa attatgattt aaataagaac cgttatatgg ccgatgcata
     1141 taaagaacgt aaatggggtt ttgttccaga ttatgcaaga tttgatatta tttataatca
     1201 tggtggattt tatttagata cagatgttga gctaattaag tcgttagata tacttcgtga
     1261 aaatcatgcc tatatgggat ttgaagggga aatctgggtt aatggcggta ttggttttgg
     1321 tgcagaagaa ggacattctg ttattaaagg tttaagggat atttacgatt cattaagttt
     1381 ttatcaaaat gatggtacat tgaacttgaa accgagtccg tattatatta cagattatct
     1441 tgaatctatt ggattggttc gcgataacag cgaacaaata ataaatggcc tgcatatcta
     1501 tcctacggat tattttgctg ccaaggatta tgatactgga aaaatttata aaactagtaa
     1561 tacaataagc attcatcaat ataatgcatc ttggaaaaca cctaaacaaa aatttgttta
     1621 tagattaaaa tcaacaattg gacaaaaaaa atttaacagg ttacttgcac tgaaaaaatt
     1681 tttacactta taaaggagaa aagtactttg aagaaaattt gtttcattag tggtgtaatt
     1741 tctcgttctg ggggaactga aagagtaggg agtattattg ccaatagctt gtctgattta
     1801 ggttatgatg tcactatttt aagcttttgg aatagtggag agccttattt tgggttagat
     1861 aagacgatta aggttgatta tatgttaaat cctaaaacgg agggaaaatt atatagaact
     1921 tatttatatc cggtttggaa attgcacaag tatatatgca aaaatgaatt tgatatagta
     1981 attgatattg atactttgtt gtctttgtat actacaaggg caattaaagg aacaaagtgc
     2041 aaattgattt cttgggaaca ttttaattac tggacaatgg aaatgctgaa agaaaagaag
     2101 agattcaagg ctaaaaaatt gataaaaaaa tatgcaaaga ggcttgtggt tctcacagaa
     2161 gaagatcgtc aaaaacatat tgatgaatat agtttatcag aagattttgt tataactatg
     2221 ccaaatccgt gtatatctaa tatcgagtac gaatatcatt atgataaaaa gagatttctt
     2281 gcagtagggc gattagtgga agagaaaaat tttgctgaca tgataagtgc ttggaaaatt
     2341 gtagaaaaaa aagtagatga ttgggatctt atcattgtag gaaaaggtga attagaaaaa
     2401 gatcttcgcc agcagataaa agatttaaat ctgcaaagag taactatagc agggcattca
     2461 gaccatgtgg aggaatatta taaatctgca tcttgttatg tgcttagttc tgtttatgaa
     2521 ggatttccta tggttatatt agaagcacag tcttatggat taccagttat ttcttatgat
     2581 tgtaaaactg gtccgagaga tttggtacat cataatttta atgggatgtt ggttgaagat
     2641 aagaatatag accaattagc taagagcatg ataatgttta ctaagaatac tgatttagca
     2701 atgagaatgt cactgaacgc atataataat gtacaaaaat ttaatttgaa agaaattaca
     2761 aaacaatggg ttgctttgat tgaaaatgta tagttttttg tggttgtcca aacatgaaaa
     2821 ttagcgtaat taatcaaaga gctagaagaa acctattaca tttcaagatt ttagaaatta
     2881 tatagtatta ggtaacaata aaaaatagaa agtttggagc catatgatat gataattaat
     2941 cgtaaacaaa atgctaaaag gaatataatt actggaacaa tttttaagtt atatcagatt
     3001 atcattccct tttttatgcg tacacttatg atttatcaga tgggaatgga gtatgctgga
     3061 ttgaacaacc tgttcacatc tatatttcaa atgttaaatt tagcggaatt gggaataggt
     3121 gctgcactaa ctgtaagtat gtataaacct atagcagaag acaatagaaa agagatttgt
     3181 gctttactga acgcatataa aaagtacttt tcaattattg gatggatagt gtttacatta
     3241 ggattgttat gcattccttt tttgagattt ttagttaaag gttcgattcc tggaaattta
     3301 aatatgacag ttttgtattt aatgtatttg ataaatactg ttttgtcgta ttggttattt
     3361 tcatatagaa agagtctaat atatgcacat caaagaaatg atgtaattaa cgtgtctatg
     3421 ctaattactt ttacattcca atttgtaatg caggcatatg ttctgatttt cttaaaaaat
     3481 tactatttgt atttattggc ttctattttg ggacaaatag ttcttaactt aatgtgtgct
     3541 gttgtttctc aaaagatgta tcctgattat actcctgttg gtgagttaga gaaagaaact
     3601 attgacgata ttataaaaaa agtaaaagga ttagttacaa acaaggtagg cggtacaata
     3661 ttacgatctt cagattcaat tattatttca tctttcttag gtctttcagt acttgcgatt
     3721 tatcagaatt attactttat tatttctgcc ataatgagta tatttgcaat aatttatgaa
     3781 tcatgtatag caggtatagg taacagtctt attgtagaaa gtcaagaaaa aaattatata
     3841 gatttttgtg atattacaac aattataggt tggtttctgg caattagttg tgcatgcttt
     3901 attacattat ttcagccgtt catgattatc tgggtgggaa gtgataatct tatgagtatt
     3961 tcatttgtta tatttatgat agttcatttt ttcttatatg aaattgatca gttaattggg
     4021 ttatacaaag atgctgctgg aatatggtac gcagacaggt ttagaccatt aattacagca
     4081 atgataaaca ttgtattaaa tatcatcctc gttaattggt tcggattata tggagttttg
     4141 gcggcaacca ttgtttcagt attgttggtt gatttaccct ggttattata taatgttttc
     4201 caaaatatat ttaaatttga atcggcaggt aaatatgttt ggaagctcat aagtttgtgt
     4261 gtaatagctt ttgttgcttc ttatttaagt tattttgctt gttcgtatgt taatatcgga
     4321 aatttcaaag tgataagttt agtgataaat ttagttatat cggttgtcat atcgatttta
     4381 gttattcttc tgtttaatat ccgtaataag aagtttaagt atattttgaa agttcttacg
     4441 tcggtattgg taaaaagatg agaagagaca tcagaatgat atataaagta actacaattt
     4501 ttaggctgcc gaataggcga acgatgtata gtttttgttc caacgaagac tcttgttgaa
     4561 attggagatg atatgaagga gttacaattt tgactcatgt ttatgattga tcggttttaa
     4621 aagtagtatg gagaagcttt gggatcatcg gagaggatca aaataggtaa taatgttttt
     4681 ataggaatga agtctagaat actaaagggt gtacatatag gaaataatgt agttataggt
     4741 gcaaatagtt tagtaaacaa ggacgtatca gacgattgtg ttgttgcagg aaatctagca
     4801 aaagtaatta tgtccctaga tgaatattat aaaaagataa acaatatgaa gaatccgtaa
     4861 aacttgtgaa actataccga gaataatatg ggaaaaatat tgatgaaaaa gcattatatg
     4921 agtttttagg gctgtttaca gatggagatg acaatttgtc gaagagttgg gagcaatgca
     4981 aaatttagta ggggattata aacagactga caaggtattt aaagaacatg cacaagtatt
     5041 tcataatatt gaagaatttt tgaatactgt gagataggag ttgctacatt gagaaaagta
     5101 tgtatttttc caatgtaaaa gattacgagt ttgaaaacga caccataata ttatttttta
     5161 agaagatatt atagagctta gg
//
LOCUS       DAFCHQ010000128         5138 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000128 DAFCHQ010000000
VERSION     DAFCHQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.132"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..448)
                     /locus_tag="LNC71_002704"
     CDS             complement(<1..448)
                     /locus_tag="LNC71_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467708.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(755..1852)
                     /locus_tag="LNC71_002705"
     CDS             complement(755..1852)
                     /locus_tag="LNC71_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBK6467709.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1960..3987)
                     /locus_tag="LNC71_002706"
     CDS             complement(1960..3987)
                     /locus_tag="LNC71_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBK6467710.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4132..4863)
                     /locus_tag="LNC71_002707"
     CDS             complement(4132..4863)
                     /locus_tag="LNC71_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBK6467711.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1040 c          813 g         1829 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aaccatccta
      721 caataataaa aatttactat ttgttatctt aaccttactt ttctttctct tctttttttc
      781 tcaaaacaaa caaaagtatg cataataaaa taaacaataa gaaaagtgaa gctactaaga
      841 tataaatcca attattgtct tttttaatcg taacatccgt cgtgttcaat tccttggcta
      901 cttttccgtc tattgtaaaa tctttttcaa atacccattg attggtatag ttgatcgtag
      961 tatttccttt ttttctcgaa aattttccat tttcattttc atttcctaaa acagtcattg
     1021 taaggtgata ttcacctggg gttagcggtt gaccatttaa agcagttgga aaagaaaaat
     1081 ttgtattcgg cgcaatctgc aatccttctt tttcctcttg atataaaact tcagaatgtc
     1141 ctttttttgt aatttttgtt tcaataacta cttgattgat ataggttttt tgatcatttt
     1201 gaacgtttgc taagatgaca tttcttgcat tgatttgatc tggtttaact tcatgaagag
     1261 taaggttcgg agcaacttcg ttcagtgtct gttgcatcaa caaagcaata atataggtgt
     1321 attcattctc aatcgataag ctttgatcct tagtttctgt ttggtccttt tccgtctgga
     1381 tttcttggaa tgtgatccca ccagccaata caccatcaaa tcgctcattc ggcatcctga
     1441 catgaaaagc aacggtttgg acacttttag gttttagcgt cactatctgt ggatagctca
     1501 cataatctac cagatcaaag atcaggctca tgtctttttc aatctcgttt tttccgtatt
     1561 caaccatcac atttgaattg gtggttgcgc tattcacaga aatatcgatt ttcacttctt
     1621 tttctgtatc atttctcaat tccgctttta cttctacttc ctgatccgga tcaagttgaa
     1681 gatcgaagta agtttttctc ttgtcgattt gattctctga agttatcggc gtcactgaaa
     1741 aattaaatgc actagctgac actgatatat tgttaaaaag tgcccctaaa ataactagga
     1801 gtgttataaa taattgttgt tttggtctca agaaaatcgc tcctttaatc atatgtaaga
     1861 gaagactata acaaaatctt gtctctgtgg gacacaagag cctatggtac ctgtgccctt
     1921 cttgaaactc aataattttg ttatattctt ctcttcctgt taatttcctg ggacatctgc
     1981 aagtatccaa gatagtttcc cctcaaaggt tccgattctt tggttttcta caggaatatt
     2041 tattctgatt ccacgatgag ctgtagaatc taaaagagag gacaaatcgc tttcaccctt
     2101 ttgatcactt tcgaaaatcg taacagcgga tgtccctata accgttgtat tttcagaggt
     2161 aacaaaggaa agagcttctg gaagctcaaa atctccattt gtaagaactt tctcttgttt
     2221 caattgtaat ttccaacttc caaaagcccg ttcatctata attttcaacc cacctgaaaa
     2281 ctgtccgaat tcacttaatg gtctactgga tattttatgt tttccaaaat tgacatattg
     2341 aggagcagtt gagagataaa gtttgccttt tctgtaataa aaatagatat cctctgtatt
     2401 ttcggagaag atgccttctg tattcgctga atccgagtca aaaacatacc cttcaatatc
     2461 aataggagtt tctttatact tctctcctat tttcccagtg attgttttca tttcagcaat
     2521 atcaatctga ctgtctttat ctttataata aacatggata tctatctcct tcagctttgc
     2581 gatctttagg tttatttcat tcgattgctc aatcaaattc ccgttttcaa ggtattcaaa
     2641 atgcatagtg agtacttttt caggaattac ttcctgcaaa gttgtctcca attcgattat
     2701 taacgtatct ccagcaataa aataatctgt cagatttagc gtgactcgat ttccggttat
     2761 caagatatct ttggcatcaa tttgatttcc attgatcaag atatgctcgg gaatagttaa
     2821 ttcttgtggg aggtcgatac tagtctgcat tttcttatat tcaagaaaat ccccctcgac
     2881 cttcaatgta tttctgatag ttatcttatc gtttcctcca gcttcagcga tggccccata
     2941 atcactatcg ttgactttta catcagctgt tgatgtgaca ttcgatggaa gtcgcgtgac
     3001 agcgacagca ttttcttgaa aaaaagtacc tgttgaagaa gtaaaccccc aatttacttc
     3061 tgtagaacca aactgcctaa tgagatcagg tataatccga gaagagcgac cgacaccttc
     3121 caaatcataa gtaagacttc tttgctccgc gtcccattta attattaaca agcgccatgt
     3181 gccattagaa agaaatgtag gagctaccgt cacgccagta tgctctccag aataattatt
     3241 attatttggt ttaggcgtga caaaagcgac atgtccatgc cctctattat cagcagttat
     3301 ctctcgatcc attcgatcag aagaaccact atttttatac gtatcaaact caatcgagag
     3361 cgcattcgca atgtaaggtt ggccagaacg agtagaatat gagccaattc ccatacctgg
     3421 cgatccaata acttgactgg gattactagc cattctagga tcgccagtca atgtaaaggt
     3481 catgccatct cccgctctat ctcgttgatt tcctaagtat aaataagatt taaatgtaaa
     3541 atccgagagt aaactaatct tcttatttga ccaaatagct cctctttgag acctttctgc
     3601 tgggttcaac tgcagtaact tcccattttc caatatggac gtgctagtac cgataggtgt
     3661 ttgaaaaatt ttatcgatag aaataatatt gggatcgtca ggcagctcaa ccgcagaaat
     3721 tggtgcatct tctagtcctt gtttgtccga accctcagct tctacgttgt cagtggtctc
     3781 tccactactc gaaatactta aatcctcact gctttcttgt gtctggttgg attctattaa
     3841 actgtcgaat gtcgaagtga gtttgctttc ttcgtctctt ctatttccat gctccaactg
     3901 attcaattcc aaagagtacc ctatgatcgg aaagatatta gtcactaaga gactgcttaa
     3961 aatgagcacg atcctcctct ttttcaatat atcttttcct cctgatcgtt aaattcctag
     4021 atgaactatt cgtatccaaa caaatttagc ctagcctttg acaatttttg ctcgattacc
     4081 tcgcaagaca atatgactac gtatttattt ttaacagttc ttttcagtaa actagttgtt
     4141 aggtgtatct tctaatgtcc aaactaattt agtggaatat tgttgcgcta acttgacaga
     4201 tttaccgggt accgaaagct gtaccgcatc ttgattttca ttttcatttg caccaaatcg
     4261 atagacccaa gtccccacac ctgttcctga tttagcatta attgctaacg tattgtttcc
     4321 tcccggtgtc aacgttaatt cagaattcac cgtttgcggc gtatatgttg gatcaaccaa
     4381 agaaacagcc tgccctttcg taaatttcag ctgtgctcca actagttcat ccccactggc
     4441 agttttaaac tgttctggtt gagagacact taatttccac ccagataagg tacttctcct
     4501 atctgtcact tggacatatg tcggttttct agacccatcg ttcatcacat cagcagctgc
     4561 ataatacgtt ttatccgcag tagaaatttt ttgagtccca aatttgaatt ttgatccata
     4621 atcaatcgat aatgcccctc ctgttccagg gcttggagga tctgatggat cgacaggttt
     4681 attgggatca ggattctctg gatcaacagg aggagttaca ccctcatctc ctgcttcaaa
     4741 tgtaatcgtt ccttctgtgt tatattcctt tgggtatact tcagttgcag aaacaaaagt
     4801 cgtacttgct accatgctga ataacatcat tgctgctgtt aatacgattt cttttttcat
     4861 cattgacatt cctctttctt tatttactaa acatatcagt cgattggatt aatccatagc
     4921 aaaattctgt tttacgatac ttatgaaata aaagaacata gacctatatt tggtcctatg
     4981 tatcgattag ttataaatga ttatcagccc gatttgattt ctagttttta ttgcaacttg
     5041 atgcaaacac aatctagaaa tacagctgtc atagccttta aaataattcg taagtccccc
     5101 tcctatctat caaaatgtga cttgacgaga gtgtaaaa
//
LOCUS       DAFCHQ010000129         5082 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000129 DAFCHQ010000000
VERSION     DAFCHQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.133"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..254)
                     /locus_tag="LNC71_002708"
     CDS             complement(<1..254)
                     /locus_tag="LNC71_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBK6467712.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
     gene            complement(254..517)
                     /locus_tag="LNC71_002709"
     CDS             complement(254..517)
                     /locus_tag="LNC71_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBK6467713.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            751..1659
                     /locus_tag="LNC71_002710"
                     /pseudo
     CDS             751..1659
                     /locus_tag="LNC71_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            complement(1718..3520)
                     /locus_tag="LNC71_002711"
     CDS             complement(1718..3520)
                     /locus_tag="LNC71_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBK6467714.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3583..4425)
                     /locus_tag="LNC71_002712"
     CDS             complement(3583..4425)
                     /locus_tag="LNC71_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467715.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            complement(4441..4662)
                     /locus_tag="LNC71_002713"
     CDS             complement(4441..4662)
                     /locus_tag="LNC71_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467716.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            complement(4805..>5082)
                     /locus_tag="LNC71_002714"
     CDS             complement(4805..>5082)
                     /locus_tag="LNC71_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBK6467717.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
BASE COUNT         1550 a         1021 c          732 g         1779 t
ORIGIN      
        1 gtccttgctg aataaatttc aaccacgtgt tcatcttcat ctaccttata aacaacccgg
       61 tgttgtctat tcaatcttct agaataccta ccttcatgtg taggttttaa cttctcaaat
      121 gattgggtag ggagataggg atcttccttt aaagtctgaa taaccttctc aaattgaggt
      181 ttcaaattag actgtttgat tttctttaaa tcagctttag ccgagttttt aatcatcaca
      241 gaatagttat tcattataag ttatcccaat caatttcatc aacattagta aatccactat
      301 catctttttc tcgcttacga acaacaccca aagtgcctat ttgctcaagt aatagcgttt
      361 ctttgattgc tttccaatca tccaaaccaa taagcaccgc attattttca ctgcgctcac
      421 taatgatttc aatttccgta tgattctcat ttacatcttt caacaactta taaaaatttt
      481 tccgtgctga gctaggattt aagactgttt ttgccatatc tactccccct ctaatttata
      541 tctccattct aacgtactta attaagtacg taaattcgat tgttttgttt aaattggagt
      601 accaaaacag cattgtaaag tgttatccgt ttcctttata cccgtggtgc tagttaattt
      661 tttcctagag gaaaatgtct aaacatgttg gtaaatcaac aaaaaaacgc atagagtagt
      721 ctcaaaactc ccaagaaatg aggactacat atgcgttcat caaaatatac tgaacattat
      781 acagatactt ttataacatt caaggaaatt atgagaactg tttctaacat ataccacaat
      841 tgtgtaccag ataagattaa aaaccgaaga aatactgatc aactgaagta acgcgataca
      901 gtgattattg cttgtgttat atggggcata attaatggct atactagcca aagagccacg
      961 tatagagcgg tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc
     1021 actcgcttaa gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag
     1081 aagctcacca aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa
     1141 ccagttagaa atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca
     1201 acaaaaaaat cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt
     1261 ccgattactt actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta
     1321 tcggaagaag cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaatc
     1381 catgaaaagc ttgcccttat gggcattact atatccattc cgccacgtaa aaacatggat
     1441 aaatcagaaa aactagacca cagcttactt ggaaaacaaa gaaaaacagt tgggactgtt
     1501 ttttcttcct tagaaaaatt aggttgtcaa aatttcaact cacgttctgt taaagggcta
     1561 gagagtagat ttgaaagcat attactggct tacagtgttc tattaagtcg agcacaacga
     1621 cgttttgaag gaactttgag atattcttta ggatattaaa attaactagc accacgggta
     1681 tctattaaga agatcctagg ttatcttaga ttatctgcta atctttgtat ttccagtagt
     1741 atcctccagc atgtttttga actccatttg cagcatccct aatacttttt gagttaacgc
     1801 caacttcttt tacagcagaa gctatagtat cgaattcttt tattattgtt ccatcatcag
     1861 tcatttgaat aatctgtctt ccacgcttct tgctaggctc actaatcgca tcctcttttt
     1921 tgttcgcttt tagaaaatca gatttttcct tagctatccg ctctgcctct tgtttagcaa
     1981 ataatagctt aattgcctta tccctacgag cctctaaagt accaacatcg tgccgtgttt
     2041 tacgcataat tccttcatca agaatgagaa tttcattatc gtaatcctta atttttcgat
     2101 aagctttagc ataagaatca tatagcgcag gagcatcata gccgttatat cgagctttat
     2161 caaaaagtgc aatagcttca tctactttgc catttttcct agcagtttct ccacgttccc
     2221 aatatggata acctgcatca aatcctttct cccaattccc tatatttcgt atcttgttta
     2281 atgaaactcg attatgcaac ccgttaataa ttacatcttc tctagatatc actttttcaa
     2341 tatttaggtt aacttcagtc tcatcatatc cacgattttt tgcatcgaat aataactctt
     2401 tcatcgctga ttttgaaata gatttcagca tactaaggct ttgctctgct tcttttttag
     2461 tgtaattact aacactcata tattcattca tagaattata acaatcgcta taagcactga
     2521 aaatataatt tgaaagaggc gttaagtcaa aggggctacg aaaatagact cgcacatttg
     2581 ttgtaccgcc ttcacttaca gtacaaggtt ctataggtaa atcaattcca tttgtagcat
     2641 cttttctaat aatgatgtaa tttcccttct tagcaaactt aaactttaag aatgtatata
     2701 gacaagttac atcgacataa ttattactgt tttttcgata cctgatccaa ctggtgtcgc
     2761 atcctttttt ctcaataata tcttgaataa aagcacaagc ttcaagttct tttatgtctg
     2821 gagtcatctt ttctatttgc tttttttgtt cttccaacga ctcctcaata atatctaaaa
     2881 caccccaaaa aatctttccg catatatctg catctgattc cgctctatgg gcagtgtcat
     2941 ttattaatcc gaaatattcc gctacagtct cttgcttatg atttacaatg ccttttatat
     3001 gttttctaga gagactcaga gtatcaacat acttaatttt agcatcatac ccaagtctag
     3061 aaaatgtgtt acttaaaaat ccaaaatcga aacttgcatt gtgtgcacac atagtgattt
     3121 ttccatctag tgcatctcct aaaaattcta ccaactgggg gtaaacttcc tgctcatttg
     3181 gagcagagtt tagcatttca ttagttatat tatttattgc cgaagctgag gctgcaattc
     3241 taacttttgg attgacaaga gttccgaatg attgaattgg aactccttct ttaaatagca
     3301 ccgcaccaat ctctacaatt ctatctgaag aagaatttag gccagttgtt tccacatcaa
     3361 aagcgataaa agttttacga aactcatcta catttgatat gtccacttcc aatttttcag
     3421 caaaaacttt accagtacga aatagactag aagccagttg ataatcaacg tgtttactaa
     3481 cctttccttg ttttttcttt agaaaatcaa ataatcccac tattatttcc tcccccatta
     3541 attttatcca gaatcacttt tacgatgcct ttttcttata ttctacctat tcgcatacca
     3601 caagaagtga tctaactttc ttcttctaaa aggaatatgt atattatttt catcaagtag
     3661 ttttccaaca cattcgttaa atgcctcgta atcaaaactt cttcgccacg tatcaatctt
     3721 attcgtagtt agtccttgga tatagtgtgg aagagtattt ttaacgatac catcaaagat
     3781 ggagtaatca tctctcccac acacttccac attatgatat gtacagtact tggatgcaaa
     3841 tgagaacata ttaactgcac caatgttcct tgcgataata tttatcaact gaggatctcc
     3901 ttttgccaac cgatcatcaa aatttggaat ctccagaatt actttagcca aatcatatag
     3961 agacaacctg ctcttatatt gagaaagatg tgttgaattt gttacatcaa ttaccgcaat
     4021 cttcatcgca atagtattta aatcattatg aagaggaaat gcattcaaaa catcatgtat
     4081 tattagacct tctttcccat aattagagct tgcacctaca aacttatgct gtttctcaat
     4141 attttcttca gttaaagata agtatttttg tttatggatt gtttcactta tagttttttc
     4201 tttacttgaa tcaagcttaa gaaccttgtc aatgcaccaa tatgttgttg ctccttttat
     4261 tggggaaata tcattctgct taagatattc ttttaataaa ctcagttgtt ttccttctgg
     4321 aatagtacca gatttcgttt ctatgatatg aggttgtggt ttggaagtac cttcattagc
     4381 agcaaataca gcatactcaa catcatttat tgttatgatt ttcaatacaa attacacctc
     4441 ctaattacct atctttattt cattgccatc aggcaatata aaagcctgca caaacttgac
     4501 acctagcaca tcagcaatcg ctttcaactc atctaaggtt accgtttctc gttgtaattt
     4561 cttattgaaa ttctgtggag tctgtccaat acgtctagca agttcggaaa cacttatatt
     4621 catttgctca cacagttctt taatcatttc tgccgtagtc atacagcacc tccaagttta
     4681 cgctaagaat attataaatc atttggttta taataacaat agttattaaa atattattat
     4741 aatcagcaag caaaagaaaa cccacgactc tactgtagaa tcatgggcta tttacttctg
     4801 gttttcatat ttccatttcg atgccagatt tgaaggatat gacgaagtgt tcttcataga
     4861 ctgtaacgct ctggattatc ttccttacaa gcttatcatc gtactctaag gtacggaatt
     4921 tgttcttgcg gataaattca atcagctcat tgattcgctc gttctcgcca ctgagggagg
     4981 catcttctac taaaaggagc tgtcgcttgt ctctcagctc atcaatctca tctgctagat
     5041 gttcataatc ttgaccctta ttagccagct tgatgagttc tt
//
LOCUS       DAFCHQ010000130         4933 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000130 DAFCHQ010000000
VERSION     DAFCHQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.134"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            85..972
                     /locus_tag="LNC71_002715"
     CDS             85..972
                     /locus_tag="LNC71_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBK6467718.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="LNC71_002716"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="LNC71_002716"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBK6467719.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="LNC71_002717"
     CDS             2274..2720
                     /locus_tag="LNC71_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBK6467720.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="LNC71_002718"
     CDS             2993..4345
                     /locus_tag="LNC71_002718"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBK6467721.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="LNC71_002719"
     CDS             4516..4803
                     /locus_tag="LNC71_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467722.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAFCHQ010000131         4893 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000131 DAFCHQ010000000
VERSION     DAFCHQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.135"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(287..649)
                     /locus_tag="LNC71_002720"
     CDS             complement(287..649)
                     /locus_tag="LNC71_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467723.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="LNC71_002721"
     CDS             complement(723..1103)
                     /locus_tag="LNC71_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBK6467724.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="LNC71_002722"
     CDS             1167..2360
                     /locus_tag="LNC71_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBK6467725.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="LNC71_002723"
     CDS             complement(2437..3021)
                     /locus_tag="LNC71_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBK6467726.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="LNC71_002724"
     CDS             3222..4220
                     /locus_tag="LNC71_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBK6467727.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="LNC71_002725"
     CDS             4332..4841
                     /locus_tag="LNC71_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBK6467728.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1553 a          914 c          852 g         1574 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttg
//
LOCUS       DAFCHQ010000132         4785 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000132 DAFCHQ010000000
VERSION     DAFCHQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.137"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            73..234
                     /locus_tag="LNC71_002726"
     CDS             73..234
                     /locus_tag="LNC71_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBK6467729.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="LNC71_002727"
     CDS             253..1530
                     /locus_tag="LNC71_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6467730.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="LNC71_002728"
     CDS             1540..2196
                     /locus_tag="LNC71_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBK6467731.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="LNC71_002729"
     CDS             2196..3572
                     /locus_tag="LNC71_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBK6467732.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3858..>4785)
                     /locus_tag="LNC71_002730"
     CDS             complement(3858..>4785)
                     /locus_tag="LNC71_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6467733.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT         1823 a          595 c          844 g         1523 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatgact
     4381 cgttgatgat ttttacttat ggtacattgg tttagcagag gacagttcac acattctttt
     4441 ggattggatt tgtattctcg gtacccttct cgagtaaccg ttgaaaaagc gagcatttga
     4501 tcttgcggac aaatataaca atcaaaatac tcgtcataga gaaactcatt ttttgataat
     4561 agctcctttt tccctttggg ccttgaataa ggcaaacaag gtcgaaggtt caaatgagtc
     4621 aaaaagtgaa caaacttagg tgtcttatac ccagcatcag cgacaacaca attgatttca
     4681 ggaaaaagtc tcttcgattg tttgacgagc tgaaaggcga cttgactgtc atgaatattt
     4741 ctaggagtga tgattgtgct aagaataaat ccattgtcgt cgcat
//
LOCUS       DAFCHQ010000133         4783 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000133 DAFCHQ010000000
VERSION     DAFCHQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.138"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            49..744
                     /gene="vanR"
                     /locus_tag="LNC71_002731"
     CDS             49..744
                     /gene="vanR"
                     /locus_tag="LNC71_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK6467734.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            722..1876
                     /locus_tag="LNC71_002732"
     CDS             722..1876
                     /locus_tag="LNC71_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK6467735.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2091..3059
                     /locus_tag="LNC71_002733"
     CDS             2091..3059
                     /locus_tag="LNC71_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK6467736.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3052..4083
                     /gene="vanA"
                     /locus_tag="LNC71_002734"
     CDS             3052..4083
                     /gene="vanA"
                     /locus_tag="LNC71_002734"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK6467737.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4089..4697
                     /locus_tag="LNC71_002735"
     CDS             4089..4697
                     /locus_tag="LNC71_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK6467738.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1477 a          916 c         1119 g         1271 t
ORIGIN      
        1 taatctgata aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa
       61 atacttattg tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac
      121 gagaattata cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag
      181 tctgagattg accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc
      241 tgtcaaaaaa taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca
      301 gaggtagata aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt
      361 cgcccactgg agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt
      421 ggagtaaagg agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac
      481 acccatgagt gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata
      541 ctgcgaatcc tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag
      601 atatggggcg acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat
      661 ttgcgcgaaa aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg
      721 gttggttata aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta
      781 catgtatatc gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat
      841 gatccgaggg aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa
      901 tcacctggac gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat
      961 ttatgtggcg attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc
     1021 aaaatacttt gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca
     1081 aattgagctt tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac
     1141 tctggaaaag cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat
     1201 gtacttggcg cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct
     1261 tgacgaggct ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga
     1321 caaagcgtat cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct
     1381 acaaacgata acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac
     1441 cgatgaattt tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga
     1501 ggatctgacc gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa
     1561 aaacgccgct gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg
     1621 ggatgtggtg tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc
     1681 catatttgaa aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg
     1741 acttggattg gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga
     1801 aagcaatgat aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga
     1861 taaaaggagg tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac
     1921 tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact
     1981 taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt
     2041 ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca
     2101 tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc
     2161 ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat
     2221 ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc
     2281 ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc
     2341 atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc
     2401 cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat
     2461 cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac
     2521 tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc
     2581 ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa
     2641 actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc
     2701 tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag
     2761 catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat
     2821 tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt
     2881 tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa
     2941 tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata
     3001 ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga
     3061 ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct
     3121 gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt
     3181 acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat
     3241 tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac
     3301 catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt
     3361 gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat
     3421 attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct
     3481 gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg
     3541 tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa
     3601 gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa
     3661 atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt
     3721 gccgcgttag ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt
     3781 catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac
     3841 ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc
     3901 ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg
     3961 aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc
     4021 gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg
     4081 tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg
     4141 acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa
     4201 atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg
     4261 ctacccaagg gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact
     4321 gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca
     4381 atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc
     4441 gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg
     4501 ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca
     4561 atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat
     4621 atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg
     4681 atttccccgt taaataaact tttaaccgtt gcacggacaa acggttctgt tgcaaagttt
     4741 taaataaaga ataaaatccc ttacggtatc tatgatttaa gct
//
LOCUS       DAFCHQ010000134         4749 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000134 DAFCHQ010000000
VERSION     DAFCHQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.139"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(136..1098)
                     /locus_tag="LNC71_002736"
     CDS             complement(136..1098)
                     /locus_tag="LNC71_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6467739.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="LNC71_002737"
     CDS             complement(1100..2539)
                     /locus_tag="LNC71_002737"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBK6467740.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="LNC71_002738"
     CDS             2724..4670
                     /locus_tag="LNC71_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBK6467741.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1511 a          813 c          779 g         1646 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacat
//
LOCUS       DAFCHQ010000135         4484 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000135 DAFCHQ010000000
VERSION     DAFCHQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.140"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="LNC71_002739"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="LNC71_002739"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBK6467742.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="LNC71_002740"
     CDS             complement(1034..1954)
                     /locus_tag="LNC71_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6467743.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /gene="xth"
                     /locus_tag="LNC71_002741"
     CDS             2067..2819
                     /gene="xth"
                     /locus_tag="LNC71_002741"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBK6467744.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3683..4216
                     /locus_tag="LNC71_002742"
     CDS             3683..4216
                     /locus_tag="LNC71_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBK6467745.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1323 a          878 c          795 g         1488 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3061 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3121 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3181 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3241 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3301 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     3361 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     3421 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     3481 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     3541 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     3601 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     3661 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     3721 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     3781 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     3841 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     3901 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     3961 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     4021 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     4081 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4141 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4201 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4261 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     4321 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     4381 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     4441 ttggacttat taccgcagat gcaagctttt ggacaccgtt tacc
//
LOCUS       DAFCHQ010000136         4441 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000136 DAFCHQ010000000
VERSION     DAFCHQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.141"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(456..881)
                     /locus_tag="LNC71_002743"
     CDS             complement(456..881)
                     /locus_tag="LNC71_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467746.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1255..2226
                     /locus_tag="LNC71_002744"
     CDS             1255..2226
                     /locus_tag="LNC71_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6467747.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2258..3655
                     /locus_tag="LNC71_002745"
     CDS             2258..3655
                     /locus_tag="LNC71_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBK6467748.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3814..4437
                     /locus_tag="LNC71_002746"
     CDS             3814..4437
                     /locus_tag="LNC71_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467749.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1554 a          714 c          904 g         1269 t
ORIGIN      
        1 tactacgtga tgtagaataa gcctttctat cgtttggtta ttttatattc aaatatttga
       61 aataaataaa tctttactct ttgatttgta aactgaaatg atccactgtt tgttttactg
      121 atgaagcttg atcaaaggaa gaagctgagt ttgaagttca taaaaatcag ccatagtgag
      181 ttttgcttgg atttgtttat cttcttctac tttttacata attttgaaca ggataagatt
      241 caaatgtttt aaatgaatct ttgactagtt tgtgacagtt gtcgcatatg ctcaagcttc
      301 cttggtcatt aaattctgta tggatatttt tgttgttatt acatgtacaa taatcaggac
      361 tatcgatcat ccatttcgtc tcctttacaa tcatcttgct tctttactat agcagaaaag
      421 aaggggagaa acaaaagaag atccaacaga cggcactata gactgtagat gttgccatcc
      481 tcatccttca aacggtaatt accgattttt gtaaatatga ctgttgtttc ttttttattt
      541 tcttttcctg aaataatata ttcgtcatct ttggtagtga tcttgatatc tgtcagcact
      601 gttttttctt cagatgattc agaagaatca gttccttgtg tctgatacgt gcgtgtccat
      661 tccatttttt tatctgtaaa tgtcaactgt cccgcggata aatccatatc tttacgtact
      721 tttagcgtat atacactgtt ggtggtatct gctttttccg cttttaagac agaagaatcg
      781 gttgtttcgt ccgaggattg gtcagagccg ccaaacaaat cacagccagt caatgtcaaa
      841 agtcccgcag acaaaataaa tagtacgatt aatttcttca aaatacttcc cccatttgat
      901 aggataaaga aattatacca ttggaatgta aaacttcaaa cggatggaag agaaaaacgc
      961 acaaaacacc cacaaataga gtaaatttta gaataaaatt ttaaaaaggc aaaaaatccg
     1021 ccactttcag aaagcgtttt aaaaaagtgt tgcaatcttt taaaaaaatg ctatattaaa
     1081 aatgaaccgg ttcaaatact tgtttaagta taattaatag tgagaggaag ttgctctact
     1141 gaagacttta gttatatgga tattgatgac aagcaacact atgcaaagaa tgcggtcatc
     1201 ataatcgaac aacaagaaag agaaatcaaa tgtaactggc aggagtgaga atgtatggca
     1261 tcgatccgtg atgtggctaa gatggctggt gtttcagtgg gaacagtttc ccgttacttg
     1321 aatggtcagc aactgaagga aaagaacatg aaaaaaatag cagaagcgat ttcagcgttg
     1381 gactataaag aaaacatcat tgctaaaggg ttgaagaata atcgaagctt ctctattgga
     1441 cttttgataa acgggatttc cagccgtttt gggtcggaag ttgtctcagg gatcgaaaga
     1501 gtagctgaag aaaatgggta tagcttatta ttaagcgggt ttgccgatca gccagaaaaa
     1561 attgaacaaa aaattgaata cctgatgaag catgtgatcg atgggctgat tgtcttctta
     1621 tccgaagaag agtggtctgg atttgaaatg ctcacaaaaa tgagtattcc agttatcgca
     1681 ttgaattgtc cagaaggacc agcaggtatt gatacaatat tagtgaatga tcgagaaagt
     1741 gtagcaaaag tgatcgctca tcatggtgaa caaggacata aaaaaatcgg atttattgct
     1801 gccactcaaa cagactacgt agcaagagaa cgtttagcag gagccaaaga agcggtccaa
     1861 aatgatccat cgatccaatt agaggtcttt acaggagact attctagaaa aagcggctat
     1921 tacggggcaa aagcattatt agaaaaaggg gcaactgcta tttttgtcag caattacaat
     1981 atgtcgattg gtgccatcga atgtttcaat caagaaggtg tcagaatcgg gaaggatatt
     2041 gcgttcagtc actatgatta tttagataaa aacgatctga gtatccttcc gaaaatcacg
     2101 atcacgccac caacagataa aatcggggaa cgagcagcag aacagctgtt aaaacgaatc
     2161 aaaaaagaag aggaagaaaa tgaaaaagtg attgtgatgg aaaatatcat acatggaatc
     2221 aactaaaacg aactaaaaaa gaaaaagagg tgctttgatg aaaaaatatc aatttccagc
     2281 aaatttttgg tggggttctg cagcaagtgg cccacaaaca gaaggaagga tttcaggcga
     2341 tggaaaagga gagaacattt ttgattattg gtacaaaaaa gaaccgcaaa agttctttga
     2401 tcaagtagga cctgaaaaaa cctcgcaagt ttatacgaag tatcaagagg atgtacagtt
     2461 gatgaaacag actgggcata attcatttcg aacatctatc caatggagcc gtttgatacc
     2521 tgatgaatct ggaacaatca acgaagaagc cgttcgtttc tatcatcgtt attttgatga
     2581 gttgattgaa aacggtatcg aaccctttgt gaatctgtat catttcgata tgccgatgta
     2641 tctgcaagaa aaaggcggat ggttgaaccg agaaacggtt gaagcctatg aaaaatatgc
     2701 caaaagttgt tttgaattgt ttggttcgaa agtaaaaaaa tggtttactc ataatgaacc
     2761 aatcgttcca gtcgaagcag gttatttata tcgatggcat tatccagaag agtcagatat
     2821 gaaaaaagcg atccaagttg gctatcatga agcgttggct tcagcgttag ccatcaaatc
     2881 ttaccatgaa atggagcaag gtggtgttat tggtatcatc ttgaatctga caccaagcta
     2941 tccaagagac gaatcaaacc ctgaagatgt taaagcagcg caaatagcag atgcgttctt
     3001 caatcgttca ttccttgatc cagctgtaaa aggcgagttt ccaagtgaat tgattgagat
     3061 agtaaaagaa cttgatctca tgcctgtgat ccatgcagag gatttgacgg ttatcaaaga
     3121 aaatacagta gatcttttag gtatcaatta ttaccagcca cgccgggtaa aagcaaaaga
     3181 aacaccaatc gatactaaaa acggtccaat gcctgaggat tatttcgaca attatgaaat
     3241 gcctggtcgt aaaatgaatc cttatcgcgg atgggagatc tatgaacaag gaatctatga
     3301 tattttgatc aatgtacgag atcattatgg aaatatcgac tgctttattt ctgaaaacgg
     3361 tatgggtgta gaaaacgaag cacgatttat caaagaagat ggcatgatcc acgatgatta
     3421 tcggatcgaa tttgtccaat ctcatctgaa atatgtccat caagccattc aagaaggtgc
     3481 caattgttta ggctatcata tgtggacatg tatggataac tggtcgtgga ccaatgcata
     3541 taagaatcga tatggcttta tttcagtaga cttagcacaa gatggaaaaa gaacaatcaa
     3601 aaaatcgggt cattggttca aagacgtatc agataaaaac ggatttgagg cttaagaaag
     3661 aatagttgag agcaaatgcg tgaaattctc cattcttacc attttgtgaa aaagaagttc
     3721 aattatttac gatctttttc aacttttttg tctttctatt tcattttttg tatataatag
     3781 aatagtcaaa attaaatagg agtggaggaa agcatgagga aaaaaagacc gtttcttctc
     3841 ttttggttat tcggtttttt cttactgctt attttatccg cagcattttc aggaaaaatg
     3901 acagcagatc ttgctcagaa agaaacagaa aaaatcgtag caagcaaaga atcagtagat
     3961 aatttcaaaa aagaattctc aacttattgg tgggcggagt ataaaataac tgccaattac
     4021 gaaaaaggcg aattggacat ttatttgcca aaaggaaaag aagaaaatca atttattaat
     4081 atcgatgacg tacttagtgc gatttctctc agacaatatc aatttaaaga caagcatttg
     4141 accgttttgc ttttgactca tgatggcgac aaagtagccc gtttgaaaaa tggaaaagtg
     4201 atcaccgctt attctgaaaa agtagacaaa aaagtggtcg ccgatcgttt agcggaatgg
     4261 aaaaagacaa atgaacaagt caagaaaaca actgtctctt ctcaaaaaaa cgagtcgaca
     4321 ggaacaagtc cttcagattc tggcagtgga caaacgataa cgggaagtga agaaaatcaa
     4381 gcctcaatta ttacgaatga gtcatcacaa ggacaatatc aatcctatgg acaatgaaaa
     4441 a
//
LOCUS       DAFCHQ010000137         4425 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000137 DAFCHQ010000000
VERSION     DAFCHQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.142"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            690..1658
                     /locus_tag="LNC71_002747"
     CDS             690..1658
                     /locus_tag="LNC71_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBK6467750.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="LNC71_002748"
     CDS             1669..2484
                     /locus_tag="LNC71_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBK6467751.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="LNC71_002749"
     CDS             2552..3262
                     /locus_tag="LNC71_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBK6467752.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="LNC71_002750"
     CDS             3298..>4425
                     /locus_tag="LNC71_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBK6467753.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAFCHQ010000138         4381 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000138 DAFCHQ010000000
VERSION     DAFCHQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.143"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..334)
                     /locus_tag="LNC71_002751"
     CDS             complement(<1..334)
                     /locus_tag="LNC71_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467754.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLE"
     gene            complement(542..1468)
                     /gene="glsA"
                     /locus_tag="LNC71_002752"
     CDS             complement(542..1468)
                     /gene="glsA"
                     /locus_tag="LNC71_002752"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBK6467755.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1491..2918)
                     /locus_tag="LNC71_002753"
     CDS             complement(1491..2918)
                     /locus_tag="LNC71_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6467756.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2949..4163)
                     /locus_tag="LNC71_002754"
     CDS             complement(2949..4163)
                     /locus_tag="LNC71_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBK6467757.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1375 a          928 c          775 g         1303 t
ORIGIN      
        1 attcaagatc agccagttct gtactcgtga atataccatt aaaataataa cttaaaaact
       61 ttaaaaaata agattgaccg tacagaaact gtgtgagctc agaaagagca ataccatgtt
      121 tgaatacgag tttatactta tgcttctctc ttacttgttc ttttatacaa tattctagac
      181 actcggctag actttctaga tcgtgaagga tcgttggtgt agtcaccctt agagaaatag
      241 ctgtttgttc gatgtctatt tcttggaatt caaataattg ttcacacaga tacacttttc
      301 taaatatatt tcgctcgatg taattttcca gcattcctga tacacgcctc cagtttttct
      361 tatgatttta tttaatcata tctgtaaatc gtatttttgt ttccttttta tttagaaaag
      421 ccctttcaaa aaccaataaa aaaagaaact gggacaaaag tcaaacaagc tcacaatttc
      481 agcctcattg taggtagctt tgagccgttt ggacttttac cccagcctct ttcaagaatg
      541 gttattcaaa gatgtcagcg tctagttctt tgactacatc tttcaacaat tggataccag
      601 ctgcgctgtt accaaatgga tctagtgccg ggctgaagac acctatgccg taacgatttg
      661 gcacagctgc catcaacccg ccgcctacac cgctttttgc aggtacacca acatggacgg
      721 aaaactctcc tgattcatca tacaaaccag cagttgtcat aattgatttg acgattgtag
      781 cactttcttc agtaatgatt tgtttgccat tccaaggagc aattccttta tttgccagca
      841 cagcagctaa ttttgctatg cctgtagcgg tgacattgag ggagcattgt ctgaaataag
      901 catcgagtat ctccggtacg tctccttcga tcatttgatt acctttcata tagtaagcaa
      961 gagagcgatt gatatcacct gttcttgatt cagaatgata gatttcttca ttcaatgtga
     1021 tattaggatc atcacagatc tctttcataa atgacaagat tcgctcaaat ttttcatctg
     1081 catctttacc ggcaatcaac gaggttgttg caatcgctcc tgcattaaca aaaggattca
     1141 ttggatgttt gcgatgattg atttccatat tcatgataga attgaaggca aaaccggttg
     1201 gttccgtgtt gattgtttga aagacttttt ctattccgtt gtctttgatt gctaataaca
     1261 atacgggaac ttttgacatg ctttcgatgg caaagcgcac atcggcgtct cctccctcaa
     1321 tcagttgttt tgtatccaaa tcataaatcg ccatacctaa ttgtttggga tccacttcag
     1381 ctaatgctgg aatgtaatcc gccacttgtc ctgtttgata atagtggaaa ttttttccaa
     1441 tcaattgttc taattctttt acttccattt gaataaccag ctttcatttt ttatttttct
     1501 gcatgctgga atttatgacg gcatgcgtag atcgtaaatg gaataactaa gacgataaga
     1561 taagaaacaa tcagaatcgt ttcataaatc ccatgctgtc cagctgcgat actagcaggt
     1621 ggaacaaatg agatacaaaa agcaaaaagt gaagtcaaaa gacctaaaca agcaacgatt
     1681 acttttacaa cttttccgcc tggaacctga taggctcttt caagactgtc atctttaaaa
     1741 accagcttca aatagcccgc gaaaaacata aagtacaaca tcaggtagat tacaacagtc
     1801 aatgacatcg ctgcaagaaa tgaaagatta ttccccccgc cgcttaatgt aaagacggct
     1861 gcccagattg ttgcaacgat cccatttgcg atcatcaaat gagcaggaac accgtgtgtg
     1921 ttttcttttg caaaatattc aggaagtaat ccttcttctg ctgctacgtg aagtgctttt
     1981 gaaggagagg ttacccaaga agcaatttct ccgatgacac ctaaacaaac aagaatcgca
     2041 agaattttcg caatccatgc aagatttgga tcaatgtgtc ttaataggaa ttctaacgat
     2101 tgaatcaccc ctgtattaag agacaaacca tgttgaggta ctgtagtagc gacggtcaat
     2161 ccgccaaaag tatctaaaat gattgccaga ataaccaata caaacattgc taatggataa
     2221 tcctttttag ggttttccat ttcatttgca tgactggcag atgtttctac gcccatatat
     2281 gctaaaataa aggaaacaaa aacaaccagc gtaggcaatt tcgaaaaatc aggaatcaat
     2341 gttttcatag acagtatcgt ttgtagtgga gcgcctgaag cgacataatg gatccctaag
     2401 ccaaacaaga ctaatgctgg caacaagata ccagctacaa aaccgatccg agcaatcaat
     2461 gaagtgtatt tggttccttt taactgtgag agtaaaatag cccagaaaat aatcaatagg
     2521 attgccaatt tcagatattt attgttttgt atagcaccaa tacccgttgc ataggataat
     2581 gcaccagtta agaaatacag catggtcaag tagccaactg ttatctcaaa ccattggaaa
     2641 aagattgcgg caaaaccgaa tcgttttccc aatgtgcgac tgacccaagt atagacaccg
     2701 cctttttccc atcctttcac cgttgccatc tctgctgcgc acaatgccac agggataaac
     2761 catagcaaac cgcctaataa caagaaaaat actaagctga agcctgaggt cgcgaatgtg
     2821 ggatattcat ataatgaaac gaccattgag atggtcatag ccaaaaagcc aaagaacgtt
     2881 aatttttttg caggaatttt cgttttgctt tgttgcatga atcttcctct tttctatttg
     2941 aatttgtttc aattgagttt gttgccaaag aagaaaaagt ccaatccttc ttcttgttct
     3001 tcttttgtag tagtgatcgt tgtaaagtgc atcgccgatt ttaaaatcac tgttgtcagc
     3061 acataggcat acacggcagt aatgatgaca ccaatcactt gaatccctaa gaagtaccag
     3121 ctgcttggat tcgttagtaa atggctgtta gatgcaaaga tccctatcag aatcgtacct
     3181 gtgaatccgc ccatgccatg aacaccccac acatcaagtg catcatccca ttgtttcttt
     3241 ttacgaaaat caacagcagc atgacagacg atacccgcaa tgatcccaat cagcatggca
     3301 ctttttgctt ctacataacc ggcacaaggc gtaatggctg ccaatccagc aacggatcct
     3361 gttagtacgt cgacaaaatc aaagccattt ccttttactt ttgcaatgat cagccaagta
     3421 atcatcgcaa atgcaagagc gatgaatgtt gaaccaaatg cattgactgc ttgttcatct
     3481 gctcgaagtg caccaccgga attaaaaccg aaccagccaa accaaagcag tcctgtaccg
     3541 atagcagctg ccataagact gctatgctga cttctttctc ctaaaggaat ctttctttga
     3601 ccaagtgtga ggatacatgc taaagagccg aatccagctg tcgtgtggat gactgctcca
     3661 ccagcgaaat ctctaaatcc catctgatcc aaaaatccgc caccccatat ccagtggcaa
     3721 acagggaaat aaatcagcag attccaaaaa ataacaagta taatatagcc ttttaaattc
     3781 aatctccctg caaaagctcc tgtcatcaaa ggaacggtaa tcacgataaa catcagttgg
     3841 taaagaaaga acattaaaaa tggaatcgtt gcaccatgaa acatgttggg gaagcttgta
     3901 acatgtctca tcaaaaagaa atctgccggg tttccaataa caccgccgat atcccgtcca
     3961 aaggccaatc cgaatccacc aaatacccac ataatcgttg ttatcccaac ggagagaaaa
     4021 gattgagaca tgatcgttag tatattattc ttccgtgcta atccgccgta aaaaaaagct
     4081 agaccaggag tcatcaggca aaccattgct gtacataaaa tcatgaatgc tgtatctcct
     4141 gtattgatag gtaatgtatt catttttttc gcctcctttc tacgattacg agtataaaag
     4201 aaggtgtagt tgtttttttc ttctgttttt ttgaattaat acttgcattt ataaaagaca
     4261 gacaattaat aaaagcttga tgtgttgtgc tctttttcgt ttttcacaca aataaagaca
     4321 aaaaataaac ggtcttcttt ttatagaaaa ccgtttattt tcgtcttttt ttatttttaa
     4381 a
//
LOCUS       DAFCHQ010000139         4361 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000139 DAFCHQ010000000
VERSION     DAFCHQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.144"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            161..598
                     /locus_tag="LNC71_002755"
     CDS             161..598
                     /locus_tag="LNC71_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467758.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            693..1577
                     /locus_tag="LNC71_002756"
     CDS             693..1577
                     /locus_tag="LNC71_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBK6467759.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1799..>4361
                     /locus_tag="LNC71_002757"
     CDS             1799..>4361
                     /locus_tag="LNC71_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467760.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1426 a          727 c          968 g         1240 t
ORIGIN      
        1 tttttattag aactaaatca acagtagttt aattattact tgatttagtt ctttttattt
       61 taaaaatttt ttttacttag aatccagcca ccctagatat ttttctgtat aatcttaaat
      121 aaagaaaacg atattaaaaa atgcacgtag aagtagataa atggagtggt tttgcaaatt
      181 tcttttattt ctagaagtta ttcacgtata cggatttgtt tttctcacaa ataaaaaggc
      241 aggaagtaca atggatcatt tgtatataaa atatattcat caattttccg ttttggtatt
      301 aggcacacta attatagcgg ggattgttgc atttattatt caaggtcgtg acatattaac
      361 agtacatcaa gagtttttca gttatgcatt tggtattcct ctgatcgcta taaggtctga
      421 taacaaaaag tggaagaaac gctattacat tctcttgcta tttttgtgtt tttatctgcc
      481 acctaagatt tttactttat taggtataca atgcctatca ttttctttta tttcctatat
      541 cattgtttta attttcctca gtttcatagt atgggtattt tatcttgtca aaaaataaat
      601 gaatgtttca tgaacagtac ttcatcacag cctaattttt ttctttatgc tatcgttata
      661 ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa agttttatta gtggtaaacc
      721 ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt agcgatagca aaattggaat
      781 cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc aggggatgca aaaaacttta
      841 ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt catgggcgga gatggaacag
      901 tcaacgaagg aatcagtgga atcgctgaac aagaacatcg gccaaatttc ggatttttcc
      961 cattgggtac ggtgaatgac ttggctcgag cattagggat accgctagaa cctgaggaag
     1021 cgatcaatca tttttcgatt gaatcagtca agccattgga tattggaaaa ataaatgacg
     1081 actattttat gaatgtcgtc gcaattggct ctatccctga agcaatcaat gatgtagatg
     1141 cggagaagaa gacgaaattt ggaaaactag cttatttcat gtcaggaatc aaacagttag
     1201 cttccacaca atcctactcg tttcacgtag aagttgatgg aaagaaggaa gaaattgaga
     1261 gcagtatctt gctgatagga ctaactaact cagtaggcgg ttttgaaaca ttacttccaa
     1321 atgcaaaagt ggatgatggt aagctccatt tggtttattt aaaagattct tctttgatcg
     1381 atacgcttaa aacattgcca gacctattaa aaggagtaga tgaatctacg aaaaatctag
     1441 cctatcttac ttgtgagcaa ttagttgtgt cattagctga tgaggaagaa ttgacgacga
     1501 atgttgatgg agatgaaggg gataaattac ctgtgacaat caaagtgtta ccctcacatt
     1561 tacaggttta ttgttaaaat ggaggttgtg accgaagagt tgtcaggagg acatcggttg
     1621 ttcggaataa gggactgtga caagactttt gtcacagcct ttttttgtac aaaaaagaaa
     1681 ttttcaccta acagaaaaat ttgccataaa aatacatatt ttaaatatga aagtacaatt
     1741 acaaataaat taaaacgtta aaataaaatt gtcaaaacgt cagagaggat ggtggagaat
     1801 gaatcaatat gtaaaaaagc tgattgcgtc gattttgttt gtcttccctc tcttgatggt
     1861 gacatccgtt tttgccgatg tagagaaaac aagcgaatca acgcaatcag agaatcaaag
     1921 ttttgacaag actacttttt cagaagaatc aattacttct gaaactacag aatcccaaag
     1981 tgaggcgcct acttctaata caaggcaaga agaaaataag acaagtgaga cctcagaagt
     2041 agctgaacaa acgtctgata gcagtgaaga ggtacaggtt agagttgttt cagcggatgg
     2101 gtacagcgaa gctgccacaa cagaagaact gacgcagctt ctcgcagacg aatcagtagc
     2161 taagattcgt ttaactcagc ctttgacttt agacagggaa ttggagatca agcgagatat
     2221 cgttatcgat tttggcggac ttgctcataa tttcggcaca catcacatct atatcaatga
     2281 gacacctcag tcgatcgagt ttcagaattt taaaggaacg gcagctcatc caggtgggtt
     2341 gataccaaat atcgatggaa atgccattat cattgcttat atgtccgatt tttggggaaa
     2401 cagatatcat tttacgggag aaataaaatt taccgggaca cttgacttat atgatggttc
     2461 taaattaggc ctgatttacg cccctcgtgc aactgtcaca ttagatggtg tttccggtgt
     2521 attggatgtc caaccagtga aagaagggat gaatgccgct ccgggtaaag cttatttttg
     2581 tagaagctat cagctaaatg taatcaatgg tagtcagctt tatggaccat atctaggaaa
     2641 attctatggt tttctagatt caggtgatga gacgggcagt ggaaaaacag caccaggact
     2701 ccatatcttg agtggttcaa aggtctctct tgactatgat cgggcagatg gcgtcagcga
     2761 tgaaggagag gctgtcgata cgctacccgg gaatgtcaca tttaaagtca gtggctcagg
     2821 aagtgaattt tcggtaaata caaagatcaa tattaccaac gacaacaatc gcggaattat
     2881 ccaaatgaga ggctcaggat caagagtcgc tgtgtcgaat gatgggaaaa tcacaatcaa
     2941 tacagcgact actggcggat ttcgcctgca aggagattct agtcaaatcc atgtatcaag
     3001 tggtggacaa atctcggtca aaatggctgg agatggcgac caacctggaa acaatgggat
     3061 gcgttttgta gggaaaggac tttctttgat cgtagaagga gccggatcag tcatcgatat
     3121 tgataagcaa agtggtcgtt ctagcgcagt tcgttttgag aacggtacgc aaaaactaac
     3181 agtaactaat ggaggctcat tgaaggtaag aaatgcaggg gatggcaaat cttatgtgaa
     3241 cagactcaat caaggaaacc aagcgatcca attttatgac gcttctggtt ttctagaaag
     3301 tccagggtca gctgatttta cggttactgg agaaaaatca aatatcgata tacgcgcaga
     3361 ttccggagct accgtagata caactggaga tattgatttg aactttatgg ctggcgaaaa
     3421 aacttattta gtcatggtcg ggaaaactgg ggaagataag actggcatca tcagtgggga
     3481 tgtgttgaat gtagacttag aatctcctac ctatttcgat ttccaaaaca aacggatcgg
     3541 aagcgagact aatggcatag gtggctgggt atttcaagga aatggcgatt cttctttgaa
     3601 gatcgctcat tcagaaattg ctttatggcg aaaggcagga gtaacgttcg ataactttga
     3661 aaaagatccg gattattttt ctgatatgac agacttatcg atcaatggaa ccgacttagg
     3721 taattttgta tcaagcccta gtgcaggttt aacaaatgaa tttgctcaga caggtgccgg
     3781 aatgaaaaat tataatcgaa tcagtgccaa taaccagctt ccgatgattg aatccttaag
     3841 agtaccaacg aatgcagata aaaaaatcta tgggcatgtc gttgttcccg aaggtgtcga
     3901 ggcaaagcca agagatgctt ggacaggcga agtagaagtg actttacgta ttacttatgc
     3961 agatacgacg aaatcacctg tagaaattac tgctctcaca caaggacaaa cagatgaaaa
     4021 tgaagggaca ggatataatc cgtggggaga aggcgaacaa ggcggtttgt ttgaggttca
     4081 agtacccaac gaagaaagac taacaacggg ggatcaagtg caaatagttg ctgctaaaaa
     4141 agtaagaggc ggccaagaag tgacagaact ggactctgcc aaaacgacag tcgatgtgat
     4201 tcctcctgag cctgctaaat taagtaccac attgatcaat gcagcttctc gctcaatcac
     4261 aggtacaggc gaagcaggag ctttggtttc attgaaaaaa aatcaagctg ttttgtccga
     4321 aacaactatt gatgatacag gtaaatttgt tctttctatc c
//
LOCUS       DAFCHQ010000140         4295 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000140 DAFCHQ010000000
VERSION     DAFCHQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.145"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..792
                     /locus_tag="LNC71_002758"
     CDS             <1..792
                     /locus_tag="LNC71_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBK6467761.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="LNC71_002759"
     CDS             889..3138
                     /locus_tag="LNC71_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBK6467762.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4295
                     /locus_tag="LNC71_002760"
     CDS             3141..>4295
                     /locus_tag="LNC71_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6467763.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1394 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DAFCHQ010000141         4282 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000141 DAFCHQ010000000
VERSION     DAFCHQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.146"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1257)
                     /locus_tag="LNC71_002761"
     CDS             complement(37..1257)
                     /locus_tag="LNC71_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBK6467764.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="LNC71_002762"
     CDS             complement(1277..1888)
                     /locus_tag="LNC71_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBK6467765.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="LNC71_002763"
     CDS             complement(1931..2863)
                     /locus_tag="LNC71_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBK6467766.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="LNC71_002764"
     CDS             complement(3284..4144)
                     /locus_tag="LNC71_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467767.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAFCHQ010000142         4207 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000142 DAFCHQ010000000
VERSION     DAFCHQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.147"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..503
                     /locus_tag="LNC71_002765"
     CDS             75..503
                     /locus_tag="LNC71_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBK6467768.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="LNC71_002766"
     CDS             490..732
                     /locus_tag="LNC71_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467769.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="LNC71_002767"
     CDS             complement(1032..1247)
                     /locus_tag="LNC71_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467770.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="LNC71_002768"
     CDS             1354..1668
                     /locus_tag="LNC71_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467771.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="LNC71_002769"
     CDS             1772..2950
                     /locus_tag="LNC71_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBK6467772.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="LNC71_002770"
     CDS             3353..3646
                     /locus_tag="LNC71_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBK6467773.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1441 a          627 c          818 g         1321 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaat
//
LOCUS       DAFCHQ010000143         4204 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000143 DAFCHQ010000000
VERSION     DAFCHQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.148"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..731
                     /locus_tag="LNC71_002771"
     CDS             9..731
                     /locus_tag="LNC71_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBK6467774.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="LNC71_002772"
     CDS             789..2828
                     /locus_tag="LNC71_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBK6467775.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="LNC71_002773"
     CDS             complement(2937..4088)
                     /locus_tag="LNC71_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBK6467776.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1532 a          781 c          814 g         1077 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgt
//
LOCUS       DAFCHQ010000144         4079 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000144 DAFCHQ010000000
VERSION     DAFCHQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.149"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(101..643)
                     /locus_tag="LNC71_002774"
     CDS             complement(101..643)
                     /locus_tag="LNC71_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBK6467777.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            778..1167
                     /locus_tag="LNC71_002775"
     CDS             778..1167
                     /locus_tag="LNC71_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467778.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1171..1935
                     /locus_tag="LNC71_002776"
     CDS             1171..1935
                     /locus_tag="LNC71_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBK6467779.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2064..3011
                     /locus_tag="LNC71_002777"
     CDS             2064..3011
                     /locus_tag="LNC71_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBK6467780.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3141..3881
                     /locus_tag="LNC71_002778"
     CDS             3141..3881
                     /locus_tag="LNC71_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467781.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1359 a          689 c          821 g         1210 t
ORIGIN      
        1 tgatgagtat atgtcgatgt tttttaatca aaataataaa taaaaactgt ctaacattta
       61 aaatttaatt tagatattag acagttttta agtttgtgag ttattttcct tgtcgttttc
      121 tcactttttt agtccagaaa ccacctgaaa tatattttgg ctcataagaa atgataaaag
      181 cacgctcttc gatttctttg atcaggcgat acagttcacg ttcgcttttt ctaggagaaa
      241 gtatttcgag aactagccgc tcaccttcca gccccattgc agaagtctgt gtcacaccat
      301 aaccgtgttc tctcagtata cccggcaaac ttttatcttc tgcgctattt gctggcaaaa
      361 tcgttgtgac catgatgtat cctaatgcca atttgtcttc aatcaaaatt ccgacactga
      421 tacctactgc gtaacctaac gcatagacaa ataaattcag tggattatct aaacgatcta
      481 aaacaaggga taagccgacg atataaatcg tgatctcgac catactgaca agtggagcaa
      541 tcagtcgata gcctttcatt gtcaacatga aacgtaacgt gttcagagtg atataagcaa
      601 aattaatgag gaagatcatc cccaacattt ttggatcaat catttgtaaa actcctttca
      661 tttatataac aacaatatca aaaaaaatcg taagtgaaga aaaattgaaa ctttatacat
      721 acaaaaaaat gataaaatga ttataataga attataaaga taggttaggt gattaaaatg
      781 aaaacggtca atcgtgtgtt ttgtgccagc cacgtgctaa gtctccttct cagtatatac
      841 ttaggtgtct attcgattca acagccaatt gaggtagaga acattgtttt tcagatgtcg
      901 ctgacagatg gtttaatttt aagcgttttt ttctttttga tactgtttat tggtaatatt
      961 ttcggtagcg ttgccagttt tttgaatttt tctatttatc cactcctttc gctagcatta
     1021 ggagtggtgg gactttgttc tctgtgctta tttcccgagc cattcagtcc tgcatttata
     1081 ttatttggta cactgaacct gttccagaca actgttggag catggttgct atggcgatca
     1141 ggaaatttaa tgaaaatagg tgagtaacaa ttgaatccaa ttatattgaa acgttctgtc
     1201 catcatattc cagtgttaga agttgttctg aaagaattaa gagaggaacg tattcccgtt
     1261 gtcatctatt atcacggatg gcagacaagt aaggagttag ttttgacgca aggaagaaaa
     1321 attgcgcagc agggcattcg tgtgatcctg ccagatgcga tgaatcatgg ggagcgcaaa
     1381 cagccggttt cttctattcc atcctataca ttttgggata gtatctacgg aaatctgttt
     1441 gaatttgaca cacttatcaa gcatttagaa aaaagaggtc ttctacatga tcagcttgca
     1501 gttggcgggg tttcgatggg gggaatgaca acatgcgcct tgttggctaa acatcctcat
     1561 atagtagcgg gcgtgtgttt gatgggttcc cctgcacctt tggaatatgg aaaagaaatc
     1621 gtaaggcgcg catcggaata taattatgtc gtaccaaaag actattttga tttgatttca
     1681 tgggttaaga gctatgattt gtcattgcag ccggaaaagt tagctggacg tcctctgttc
     1741 ttttggcatg gacaagctga cgaaaaaatc ccttatatac aaacggaacg atttgtagaa
     1801 aaaaatcggc ataagtctta cggaaaagaa attgtttttc gttcctcaaa aacacagcga
     1861 catatggtac aaataccact aatggaagaa gcttccactt ttttagcaaa aaatttgata
     1921 aaaaagtata aataattgaa acggaaaatc actcatgacg atagaggatt ttccgttttt
     1981 tattttgggc tataataaac tctgttagga aaatttggta taattgatag taagaatgac
     2041 aacatgttag aaaggaagag agcatgccca ctttagcaga tgttgcaaaa cgtgccaatg
     2101 tttcaaaaat gacggtctct cgagtcatca atcatccgga acaggtgact gacgaattga
     2161 aagagttagt tttctcagca atggctgaac tggattaccg tccaaatatt gctgcaaaag
     2221 cacttgtcac gaatcggtcg cagatcatca aattatttat actggaagaa atcgacacaa
     2281 ctgagcccta ttacatgaat ttgctgatgg gaatcgctaa acggatcggt aaagcgcatt
     2341 attctctcca attagttaca aatgatgcct tcgacgtcgg ttcttgtgat ggatacatca
     2401 tcacaggtgt cagaaaaaaa gacttcgatt ggatcgaccg tttggaaaaa ccagttgtct
     2461 tgtttgggga aaattctgaa ggatatgatt atgtagacag cgataatgaa tatggtacag
     2521 caaaagcaac gcaatatgcc ttagaaagag attatcaaac gattatttat atcggtatcg
     2581 aagtggacga gccttttgaa atttcaaggg aaactggtta tttatctgtg atgaaggaag
     2641 ttgatcttcc tagtaaaatc ttgcggtttc caaatcattc gacagaagta gaaagatata
     2701 tgacagaaaa ctgggcagat tttacagata aaacttgttt tgtatgtagt tcagatcggc
     2761 tagcactagg agcagtaaga gggattataa atgctggagg acacttgccc gaagaatttg
     2821 gtgtgattgg atttgatggg gtatttttag accaaatcag ttcacctaaa ttgacaacga
     2881 tcaagcaaga tattatacgt atgggtgaag tatgtggaga aatgttgtta aaaaaaatag
     2941 aagaaaaagg ggcgagtcaa ggttatcgtc gtttttttcc tgaactagtt atcagagaga
     3001 caacgaatta agtcttatta ctatatatat atttatgaaa ttcatcgcta ttttcttaaa
     3061 ataatcagcg cattgctact gaaaacaatg aatctgtgct attattgaga aaagttccat
     3121 ttttgagaag gatggtttga atgaaagaaa tcagacgaaa acatttccgt tttcctgcgt
     3181 acgatgatga gttaggcgta aaattaacca acgacaagaa gcgagtatta tttgacggac
     3241 aagaagatct tttgactgaa cagaatcagt ccccgtttga acagatgaaa gaatttcatt
     3301 tcacgaacac gaacaccgag aaaatccgtc gcaagcgtcg tgcaacaaat ggattgaagt
     3361 cagctccgcg taaggaaaat ttggaaaagc ataaaggaaa attaccggat tataccggcc
     3421 attaccaatc agaaatcaca tcgacaggac gaaaaaaact ttttggtgat tcctctcgtt
     3481 ctgtcagaca aagtgaaaca aggacagagt ccaagcatac tacacgaaac gcatctgttg
     3541 acacgaaggt gcgggagcgt tcttattttg ttcctaaata tgtacccgca tcgattatcc
     3601 cagaccctag agaaagcaaa atttctgagg atcatttaat cgaatcgatg aaaaaaacgg
     3661 atgactctta tctgttgttc gatacagaac cgacgccgta tcaagaaaaa agaaatggtg
     3721 agccgtctgt acgaacattc aatcgaacaa aaacgttaga ggaaacaaag cagtcagaag
     3781 aaaaaaaatc gaaaaaaagc catggtatct tagaacgctc attacaagga ttgatcgatg
     3841 atcaaggaaa ctctttggga gaaaatagct attttaaata agtgatatac aaatttatat
     3901 tcggctacag ttggaaagga aacgctgcgg cgtttcctta ttttatactc tatccgaaag
     3961 tttatatcgt atcctcaaat ttttttgagt ttaagcgaat tttttctccc aataagtaag
     4021 aaaataatgg caaaataaaa aaacaaacta ttttcgcaga tcgtttgcaa aaatagttt
//
LOCUS       DAFCHQ010000145         4047 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000145 DAFCHQ010000000
VERSION     DAFCHQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.150"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(156..1661)
                     /locus_tag="LNC71_002779"
     CDS             complement(156..1661)
                     /locus_tag="LNC71_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6467782.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1658..2344)
                     /locus_tag="LNC71_002780"
     CDS             complement(1658..2344)
                     /locus_tag="LNC71_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6467783.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2537..3958)
                     /gene="gndA"
                     /locus_tag="LNC71_002781"
     CDS             complement(2537..3958)
                     /gene="gndA"
                     /locus_tag="LNC71_002781"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBK6467784.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          856 c          692 g         1383 t
ORIGIN      
        1 gttcatctaa aagatactta catttaatta acggatgtta ttctatttaa ttgttcgcta
       61 gtttttttat aatgacatcc ctgaatttct cacttgcttg tattttgtac cttattcttt
      121 gttggatata taaaaaaaca agtaaagcac atgctttact tgtcttctgc ctttgcgata
      181 ggaataaaga ttctaaaaat tgtcccttgc cctactacac tttctgctaa gatacgtcct
      241 ttatagtttt caaccagttg tttagcgata gacaacccca gaccatttcc acccttcgtt
      301 ctagctcttg cattatccac tcgataaaaa cgattgaaga tcttattaag gtcttctttg
      361 ggtattcctt cgccaaagtc ctgaatcgcg atctcaaatt cattcagagt ggaagaaata
      421 gagatatgga cttcttttcg tgtagttgaa tactttactg cgttatccaa tatgataatg
      481 attaattgtt caaagtgatt acgataaatt tgcagggtca cttcttttgt caaatcatca
      541 tctaaagtga tcacatagtc tggatataat atcttgaaat tattataaac ttgataggtg
      601 acttcttttc ctttagttgt ttcattcgca taatgaacat ctacttgttc tgctcttgat
      661 aagtctagca tctcttgaac taagcttttc atacggctga tttcttgcat gctggcgctt
      721 aatgattctt ctaatatctc aggatcatct ttcccccaac gatttagcag actaagatgc
      781 ccttcaataa ttgcaacagg tgtccttaac tcatgagata cgtcttccac gaattgctct
      841 tgttgttcaa tatacaaacg catacggtca agcatctcat tgaatatttc agctaaatct
      901 gccaattcat catttgtgtc gatttcaggc atgtggatat ctgattgcgg atctttacgt
      961 atagtatcca ttgtatctct gagtaccttg agtggtttta agaaataaga agataaaaag
     1021 aaacctaaaa tactacttaa gatcaaagaa acaacttcta aaacaataag agtcaataat
     1081 agcttatttc gaatatcata gaaagaagat aattcataga agctttgcgc atagccgatc
     1141 ttttctcttg tacctttaga atatataggc tggatagata aaaagccagt ttttccttct
     1201 agtgtaacga tcgttggaac tttacgtgta gtctgaatca ggtttcgact gttttcatgg
     1261 gttttaaaaa tcaagttctc gtccaaatca taaacataaa gatccattga tggctgccct
     1321 agctcagcta taaaggaatc gatcttcatt aagtttcctt cgaccagtga agtatgatct
     1381 acgacctcat ctccgtcatt tgtcgctttc gtcagattac gatacgtatt tactaatgtc
     1441 agttcttcat ccgagttagc caaacgagaa gctacttctg aaatcgtacg ctctacattg
     1501 tttctttctt ttgccacaat cagattgata gatgatttat aggtaatgac agcgaaaatt
     1561 gtaaacacaa cgaatatgaa gaaagaactc gcaaaggccc acttgattgt caaggagggc
     1621 cctttcagtt ctcttttttc cgattttttc ggctttatca cgagcgcatc acataacctg
     1681 ttccgcgtac agtttggata tagctttcct ccccgggaac gtcaattttg ttgcgaagat
     1741 aacgaatata tacgtccact acgtttgttt caacttctgt ttcgtatccc cacactttat
     1801 ttagtaaaac gtcacgtgct aataccacgt tcacgttttc cattaaagta agcagcaact
     1861 cgtattctct ttttgttagt tcaattactt catttccgcg acgtacgaca cggttttctt
     1921 tttcgatagt caaatcgcga taagtaatag tagtttgttt agccacattt ttatcgcctt
     1981 caatatcgat tcgacgcaac aatgcacgaa gacgtgctaa taattcttcg atagcaaatg
     2041 gtttgacgat gtagtcatct gctccgtggt caagtccaga tacacgatcg atcacagaat
     2101 ctcttgcagt catcataatg attggtgtat ttttgacttg gcgtacacgt ctgcagactt
     2161 ctaaaccgtt caattctggg agcatcagat ccaacaatat ggcatcccac tcattattta
     2221 atgccgcatc taaaccagtt cggccgttgt agtggacttc tgcgttatac ccttcatgct
     2281 tcaactctag ctcgacaaat cgtgccaagt tcttttcatc ttcaatgatt agaatattgc
     2341 tcatttatta ttttatcctt tctattctac tggcttttta aaaactctat cattataccg
     2401 ataatatgct taaaaatcta tagtcatttt atcatggata gcaattttaa aaaagagctg
     2461 aagtctactc ataacaatcg acttcagctc tttcgtcctc tttttatttt ctcatttcct
     2521 aacagccgaa agactattat tcttcatggt accatgagta atggaagatt ccttctttgt
     2581 cgacacgctc ataggtatgt gcgccaaagt aatcgcgttg ggcttggatt atatttgcag
     2641 gcagacgttc tgctctgtat gaatcgaagt aagcgatggc tgatgagaaa gtcggtacag
     2701 gtacgccagc ttggacagcc aaggcaacga cgtctctgac tgattgctga tattttttcg
     2761 tgatttcaac gaagtaatca tctagcaata aattttcgat ctcaggattt ttgtcatagg
     2821 catccgtaat tttttgcaag aatcgagctc ggatgataca tccagcgcgc cagatttttg
     2881 cgatttcacc aaatggcaag tcccaatcaa attcttttga agcgacacgt aattgagcaa
     2941 atccttgggc ataactcatg attttactga aatacaacgc ttctcggatt ttttcgacca
     3001 attctttttt gtctcccgtg aactcaaaat cgttcgtacg agacaaaatc ttgcttgctt
     3061 gtacgcgttc ttctttataa gcagaaatat agcgggcaaa tacagattca gtgatcaatg
     3121 gaagtggcac acctaagtca agcgcgcttt ggcttgtcca tttacctgtt cctttattcc
     3181 cagctgcatc aaggatcaca tctactactg gtttacctgt tccttcatcg tctttacgtg
     3241 tcagaatgtc agcagtgatt tctatcaaat agctgtctaa ttcgccttga ttccattctt
     3301 taaagatatc agccatttct tctactgaaa ggtttaaaat attcttcatt aagtcataag
     3361 attcagcaat cagttgcata tctccatact caataccgtt gtgtaccatt ttcacataat
     3421 gtccggctcc atttgggcca atatatgtga cacatggtgc tccgtcttct gcttttgcag
     3481 aaatttgctc taagattggt gcaactagcg cataggcttc tttttgtcca ccaggcatga
     3541 ttgaaggtcc ttttaaagct ccttcttcac caccggaaac gcctgtaccg ataaagttga
     3601 tgccagagtt ggctaactct tcattacgac gcatcgtatc tttaaagaat gtatttcctc
     3661 cgtcaatcaa aatatcgcct ttatcaaggt gaggaagtaa ctcttggatt gttgcatctg
     3721 ttgccggtcc tgctttgacc attagcaaga tccgacgtgg tttctcgata gattctacaa
     3781 attcttcgat tgtatatgtt gctttgaaat ttttatctgg gtgttcttct actacagcgg
     3841 ttgttttagc ccctgtacga ttgaataaag ccactgaata gccgcggctt tctatgttca
     3901 aggctagatt tttacccata acggccatac cgacaacgcc gatttgttgt tttgacatat
     3961 gaactcctcc tacatacggg atttctccct aatctcttac attatagctt aacacggcta
     4021 cattgcaaac taaagaacaa catattt
//
LOCUS       DAFCHQ010000146         4030 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000146 DAFCHQ010000000
VERSION     DAFCHQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.151"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(243..392)
                     /locus_tag="LNC71_002782"
     CDS             complement(243..392)
                     /locus_tag="LNC71_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467785.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="LNC71_002783"
     CDS             854..1066
                     /locus_tag="LNC71_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467786.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="LNC71_002784"
     CDS             complement(1344..3143)
                     /locus_tag="LNC71_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBK6467787.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="LNC71_002785"
     CDS             complement(3267..3863)
                     /locus_tag="LNC71_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBK6467788.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1181 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt
     3961 gtaagttcat cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt
     4021 taataacttg
//
LOCUS       DAFCHQ010000147         4027 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000147 DAFCHQ010000000
VERSION     DAFCHQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.152"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..518
                     /locus_tag="LNC71_002786"
     CDS             21..518
                     /locus_tag="LNC71_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467789.1"
                     /translation="MSYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTL
                     IAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLC
                     LVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYF
                     YRNKL"
     gene            complement(644..1321)
                     /gene="nth"
                     /locus_tag="LNC71_002787"
     CDS             complement(644..1321)
                     /gene="nth"
                     /locus_tag="LNC71_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBK6467790.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1328..2026)
                     /locus_tag="LNC71_002788"
     CDS             complement(1328..2026)
                     /locus_tag="LNC71_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBK6467791.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2294..3478
                     /locus_tag="LNC71_002789"
     CDS             2294..3478
                     /locus_tag="LNC71_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBK6467792.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3484..3927)
                     /locus_tag="LNC71_002790"
     CDS             complement(3484..3927)
                     /locus_tag="LNC71_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6467793.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1059 a          785 c          655 g         1528 t
ORIGIN      
        1 ttttgaaaga aggcttcatt ttgagttatc tgatagtatg cttaattatc ataatttgtc
       61 tgcaccatct ttttttatcg aaaacaatac ctcgttattc caagaataaa aaagctgaaa
      121 aattttgttt attattaaat aaatttactt taattgctcc tatccttgca tttattattt
      181 tttctgtact tttaagcact actcttaaag gaaaatttat ggaaagaagc tctcatgcaa
      241 tgattctcac ttttttatgg ttgttgttca ccagaatcta tatttttctg atgagcctga
      301 aaccgcccaa atccattagt ttatgtttag ttataaatgg tattttttta ttatcactta
      361 tcatttttat tacaccttta gatagatacg tcacttattt atataatcct ttggaatact
      421 ggacttattt tataggtata ttggaaggca ttattttcta catcgggtat tttcccaata
      481 aaaataacaa cttttatttt tatcgcaata aattgtaata agttaccatc ctagctactg
      541 agtatgtaca tttacttttt catgcaaaaa agataaacga aacagctttt ctccataata
      601 acaagataat tcatacattt atttgcaatt aaagaaacca cgtctacagg cgtggtttct
      661 tttttagcgc cttttctttt agacgcatcc gatttttccc atcttgacat attgatagca
      721 aaggacatac ctcgcacttg ggatttcttg ccgtgcaatg atatctacca aaaaaaatca
      781 gtgtatgatg tgtctttacc cataattctt gtggtacttt ccgcatcagt gtttcttcta
      841 cttccatgac cgtagcatct agtttgcaga tcctcaggcg ttttgagaca cgttccacgt
      901 gggtatctac tgcgattgct ggtatcccga atgcatcccc caatacgaca tttgctgtct
      961 ttctgcctac accaggaaga gacatcaatt cttctcttga cgttggcact tgcccatcga
     1021 aacgttcaat cagctgctgt gcacacgctt tgatgttttt tgctttattt cgatacaaac
     1081 caatcgtttt tattttaaga atgatttcat cgattgaagc ttctgctaac gcatcgggtg
     1141 tcggaaaaga ggcaaacaaa tctggcgttg ctttattcac tgatacatct gtcgcctggg
     1201 cacttaagat cactgcaatc aataactcaa atggattatt gtgcttgagt tctccatgtg
     1261 cttcgggaaa cattccatac atcgtttcta gagcttccat tgttttttgt ttgcttaaca
     1321 tattccgcta ctcctccaac caatttttca gtgagacttt cggcaattct tcttgttgag
     1381 ctgactgttc tacttctctt tgaagaagct gctgttttct tcgtttttgg tcttcttcca
     1441 cttgttgttt tgtacgtaga ttttttcttt cccacgatag caaaatgcgg tcaacgtaat
     1501 taaaactata agcctgattt aatacggctt cccgcagtgc tagcttcaaa atttccggct
     1561 gataatggtc ttcttctagc cattgtccga tccgctggaa ctcgatcgaa gaaagcgggc
     1621 gtccaaattc ttgttcaaat aattgataca ctgactggat ttctttttct tgtgtttttt
     1681 cttcgtattt tttttcttgt gtttttaaat actcacctaa tagatcatag atggggtaaa
     1741 gattatatcg atctgctttt ttaccgttga caatcgtcgt ttctatcttg ataaatccat
     1801 ttgttacgag acgatccagt atttgaaaaa tctgatcttg tttgattccc atatccatag
     1861 caatcatctg caaatcaggg aaagtatctc cttcaagctg tgccatatgc aactgtagaa
     1921 caaataaaaa ttcttcagat tgtagaccga tcctatgata atttttaagt aaaagattag
     1981 atatgactgt ttgtccagcg cttaagtaat tttctaaatt caacatgatt tctcctccgc
     2041 tcaatcacag tatacgtaaa aaaaacatgg gaagcaagaa caggtttctt ttctatttac
     2101 ttcactgatc ggtcagattt acaatgtcgt ctacttgatt tttgtttgtt catgtttttg
     2161 atgcgtatct tcacttttcg gaataaataa attaagtaga tttcgctgtt ccttagaatg
     2221 ttgacaagga aacaaaatta ctatacacta tttttgtttc caagttaaca ttattgcata
     2281 aaggagtttt tacatgtaca gtttcacaaa taaacattct aaagtttttg gatacgtctc
     2341 tgtcttattc actggattag gatttactat cgtcagtcct gtacttccgt ttttaacgct
     2401 gccttattct cactccatac atcagcaagc tttctatatc actttgctca tgtctgttta
     2461 tgctctagct gccttcttat ctgcccccat tctcggtagc ttaagtgatc attttgggcg
     2521 tcatccgatc cttattatca gtttattagg atcaagtatc ggatatttgc tttttggttt
     2581 aggaaattcg atatggatgt tgttccttgg tcgtatcatt gaaggattga cggcaggaga
     2641 aatctccacc ttatatgctt actttgccga catcacggaa cctaatgaaa gaaccaaagt
     2701 ttttggatgg atgggtgctt tagtcggtat aggaacaacg ctgggtccta ttataggggg
     2761 tctattagct gagttgggga attctgttcc tatatttatt ggagcactct ttactttctt
     2821 aaatgccgtt tatggctaca catttatgct agaaagcctt ccaataaaaa aacgttcagt
     2881 agatttaagt ttctcccatg taagaccttt tcaccaatta caacaattat tcaagatttc
     2941 atccgttatt cctttattga cagcaggttt tgctgtttgg ttggcagctg gctctcttca
     3001 atctatcttt tctcaatttt cgattgacgc ctttcagtgg aaggctgggt tggttggttt
     3061 aagcttctct ttgattggaa tcttggatac tgtctcgcaa ttgttcatta tgcctcgatt
     3121 gctcaaaaaa ttttcagaac agcagatcac aagaataggg atgttcagtg aaatcctcgc
     3181 ctatctattc atcacattat ctggaattct tctactgccg attttgtttt tatttgggat
     3241 tatctgttat ggtttcggtg attctatttt tactcctgta ttcaatggcc aactatccaa
     3301 ttctgtctca gataaccagc aaggtcttgt aatgggcggt acccaaagta tacaatctct
     3361 atcgcgcgtc atcggcccgc taatcgcagg acagctttat gctgcagcac catttcttcc
     3421 gacggtattc ggatttttgc ttatgatttt tgcctattat cactacaata aaaaatagta
     3481 gcttcatttg ctacttcgta taaattctct gtatcgaccg ataaaacgaa atactgtgtc
     3541 aaattcttct tttgacatat tttcaaatac agtagcatct cgctgtaccc atttttgatg
     3601 gagttgctcg tgtctattcc ttattttttg tccgattggc gtcaattgaa aatagatttc
     3661 ttttttattc tcttcgcttt gataagtgtt tatgatccct ctagctatca gtttttttgt
     3721 tagcttactg atggctcctc tagtcatgta actagcattc gctaattttg tcacgttagg
     3781 atcagggatt ttcccgatat gttcaatcac ttctagttca tttagagtaa atcctgcaaa
     3841 ttctttttcc atttctggtt tattacgatg aataatctca ttagctaaat ccagatattc
     3901 ttctattagc agttgtcttt tatccatttc tacaccttct ttttctttca ttatattgtt
     3961 tttttgtttc ctattcaaca aagataaaaa tattttgtag gaaaacccaa tctgaaacca
     4021 agaaaaa
//
LOCUS       DAFCHQ010000148         3946 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000148 DAFCHQ010000000
VERSION     DAFCHQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.153"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            695..1735
                     /locus_tag="LNC71_002791"
     CDS             695..1735
                     /locus_tag="LNC71_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBK6467794.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2559..2891
                     /locus_tag="LNC71_002792"
     CDS             2559..2891
                     /locus_tag="LNC71_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBK6467795.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3510..3851
                     /locus_tag="LNC71_002793"
     CDS             3510..3851
                     /locus_tag="LNC71_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBK6467796.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1445 a          502 c          716 g         1283 t
ORIGIN      
        1 tgggtagttc ttatttttta cattttaagt agtatttaat tgcaatttgg tagtattttt
       61 ttattatctt attcttagaa cggtagtact taatataaat ataggtagta tctaatttaa
      121 gtattaatgg tagtatctaa attaacaggt agtatgtaac tactttaaag taattttttt
      181 cagattaaca cagtagtatc ttattaaata agttaatttt aatggtagta cataattatt
      241 atatatatct gacattggta gtacttattg tattttttag tagtatataa tctattgttt
      301 tctagcctaa agccttgtct tataagaata atataatata tactactgaa ataaggtagt
      361 atatattact agtaatggta gtatgtttta tatgcataag gtagttctta atttattttt
      421 tgttttgaca ctggtagtac ttaattatct ttaattttaa aactaaattt tattcatacg
      481 tttttctggt agtatttaat tataaataat atttatttaa agtagttctt aatgacttta
      541 aaggtagtac ataattcatt ttatttctga ttttaggtag tatttaatta aaaaacagtt
      601 ttaatgcgtt tgcatttttt atttatcttt gctattttcg aaaagagtag gtaattttac
      661 ataaataaaa caacgtgaaa gtagaggatc tacaatgact gattttaaat tttttaaggc
      721 ggatagagtt tacaacgaat tattttatca atttccaaaa gtctttattg tttctgacga
      781 atacaaaaaa atgaaagatt caactaagat tgcctatatg cttttaaaag caagattaga
      841 gatcgcaatc agcaaacggc aaatcgatga agaaggtaat gtttatttta cttatacgac
      901 aaatgaacta tgtagagtat taaactgcca aaaacaaaaa gcgatagcaa tcaaaaaaga
      961 gttggaatcc tttggtttat tattacaaaa gcagatggga tttaacaaac agttagggaa
     1021 aaataatcct aatagactat atctagcaga attaaaagtc tcagaaaatg atatctactt
     1081 actcgaaaaa tttgatagag agaataggga aaacgttgat aaatcagagg gtatgaaaat
     1141 catacccacc ctcgacgaaa aatcagacgc tgaatccctt ggggctcaag agggtatgaa
     1201 aatcataccg tgccaaaacg ttgataaatc agagggtatg aaaatcatac cagaacttaa
     1261 taataatata ttagacacta atagacacaa tatagacact gaaaaagacc gcctacaaga
     1321 tcaattgttg ttagacaatt ttgagacaat tatgacaaac gacagcattg ctacgtttgt
     1381 ccctgaacga gtattaaatt tgataaaaac attttcttca agttacagtg aagctcaaaa
     1441 aaccgtccag actattcata atgcaaagaa aaaagctgaa atagaaagtg atatttcgat
     1501 agtttttgaa gaactcgata gttattatgt caatgcagaa caagaattat acacgacact
     1561 gttaaaagcc tatcaaaaat taaaaaccga aaaagtcgaa aatatccaga acctgatttt
     1621 tgtctatgta aaaaattggt ttatcgaaaa accaatagct gctaaagtat caagtgaaaa
     1681 acgtttgaat tatgcaagct ccccaagcac tattacgaaa gactggttag agtgaaactt
     1741 tactgacaaa gtgattgttg cttcatggag taacattaaa attaaaaatt ttttattatt
     1801 ctaattatat tacgaatatt acaaaataat agctatttta atagaattta taagttaaaa
     1861 tattatgata aaaatcacaa acgtagcctt ttacgtcata agtgttaaaa gaaataatta
     1921 atttcaaata tagaaaaggg aacgtatgtt tgcatactcc ttttttttga gttataataa
     1981 tacgcgaaga gagataattt cattatttaa acaagctaaa aatcttgata ttacttcttt
     2041 tcaggcaata cgaatattat tcgcttacat tttttgtata aatttaattg tctgctatgc
     2101 ttgtgatgaa ttgattttcg ttttgcactt ttggatgata aatttggaca attgaaaata
     2161 caaaaaaaag aaaggcggta tgaactcgga aggctggtaa ccttcgtcag cgttcataca
     2221 cggcattaag atttggccct taatgcgtta acctttcagt gttacggtat tacccgcaat
     2281 ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg ttaaggttaa
     2341 aaatacgtga tattacgatg aatctatgcc taagcataat gttataaaaa tgtccgtgtg
     2401 gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga tgcgttccct
     2461 cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac
     2521 ttgttaaaaa tatttaaaga aattgggtgg tttattttat gtcatataca ggaattttga
     2581 gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga
     2641 aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag
     2701 acaaagtctc agaggaaatg atttattaca ttgggaaaca aaagcaaata attttaaaag
     2761 atcctatccc cttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg
     2821 tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt
     2881 tgatcggctg aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa
     2941 atattgtcga gacttctaaa ggaacttaga gtcactagaa aaataaggag tagacttgat
     3001 ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt aacgtaaaaa
     3061 attggtcaat cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt gtgtaaataa
     3121 taagcgcaga gtaaacaaga gggaatttag aagttagaca agttagaata gcgtctagat
     3181 taatcatgag cagctactaa gggatctaga ctttgacgca aactcatgga ataaggagta
     3241 ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag
     3301 atacttgcga ggacgttgat tattggatca atcgagcatt taccgctgtc tactagggtt
     3361 tctcgtttcg gacgccataa acgagtcgaa attttttatg tatttttaag taaacgaaaa
     3421 acgataaaat atgaacaagt cctgaataaa gtgaaaaaat taatataaag catagagaaa
     3481 atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat atacgattgg
     3541 gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt gtgacgttta
     3601 gaaggggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat gatttatacg
     3661 atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat gatgaaggta
     3721 atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct gttgaagata
     3781 cttggtcata tttgagtgat tatattgtca gctttgaaat cattgaattt gtagcggata
     3841 aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag gagtcacgac
     3901 gattaataaa aaggtagtat taccttaggc attgagtaag gaattt
//
LOCUS       DAFCHQ010000149         3925 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000149 DAFCHQ010000000
VERSION     DAFCHQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.154"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(573..1130)
                     /locus_tag="LNC71_002794"
     CDS             complement(573..1130)
                     /locus_tag="LNC71_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467797.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="LNC71_002795"
     CDS             complement(1309..1920)
                     /locus_tag="LNC71_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467798.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="LNC71_002796"
     CDS             complement(2033..2290)
                     /locus_tag="LNC71_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467799.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="LNC71_002797"
     CDS             complement(2887..3165)
                     /locus_tag="LNC71_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467800.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="LNC71_002798"
     CDS             complement(3199..3450)
                     /locus_tag="LNC71_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467801.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAFCHQ010000150         3863 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000150 DAFCHQ010000000
VERSION     DAFCHQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.155"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(245..466)
                     /locus_tag="LNC71_002799"
     CDS             complement(245..466)
                     /locus_tag="LNC71_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467802.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="LNC71_002800"
     CDS             complement(564..809)
                     /locus_tag="LNC71_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467803.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="LNC71_002801"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="LNC71_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="LNC71_002802"
     CDS             complement(2033..3034)
                     /locus_tag="LNC71_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBK6467804.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="LNC71_002803"
     CDS             complement(3193..3459)
                     /locus_tag="LNC71_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467805.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="LNC71_002804"
     CDS             complement(3449..3805)
                     /locus_tag="LNC71_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6467806.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAFCHQ010000151         3845 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000151 DAFCHQ010000000
VERSION     DAFCHQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.156"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1364)
                     /locus_tag="LNC71_002805"
     CDS             complement(45..1364)
                     /locus_tag="LNC71_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBK6467807.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="LNC71_002806"
     CDS             complement(1361..2014)
                     /locus_tag="LNC71_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBK6467808.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="LNC71_002807"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="LNC71_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="LNC71_002808"
     CDS             complement(2724..3440)
                     /locus_tag="LNC71_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBK6467809.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3490..3699)
                     /locus_tag="LNC71_002809"
     CDS             complement(3490..3699)
                     /locus_tag="LNC71_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467810.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
BASE COUNT         1235 a          727 c          479 g         1404 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaag
//
LOCUS       DAFCHQ010000152         3844 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000152 DAFCHQ010000000
VERSION     DAFCHQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.157"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..888
                     /locus_tag="LNC71_002810"
     CDS             <1..888
                     /locus_tag="LNC71_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBK6467811.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            1000..1896
                     /locus_tag="LNC71_002811"
     CDS             1000..1896
                     /locus_tag="LNC71_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6467812.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1994..2203
                     /locus_tag="LNC71_002812"
     CDS             1994..2203
                     /locus_tag="LNC71_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBK6467813.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2221..2493
                     /locus_tag="LNC71_002813"
     CDS             2221..2493
                     /locus_tag="LNC71_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBK6467814.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            2495..3358
                     /locus_tag="LNC71_002814"
     CDS             2495..3358
                     /locus_tag="LNC71_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HBK6467815.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1553 a          584 c          659 g         1048 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc ataaagaggg cttttatata ttaatcacaa atcacttatc acaaatcaca
      961 agtgattaat cacaaatcac ttgtgatttg tgattcttaa tgatacaata ttactataca
     1021 aaaaaagaat ggggcgtagt tatggagaag gaaaaactaa aaatacttga agaattaaga
     1081 cgtattttaa acaataaaaa tgaagcaatt attatcttga ataattactt taaaggtggt
     1141 gttggaaagt ccaaattatc gactatgttt gcttacttga cagacaaatt gaatttaaaa
     1201 gttttaatga tcgataagga cttacaggca acattgacaa aagacttagc aaaaactttt
     1261 gaggtagaat tgccacgtgt caatttttat gaaggcttga aaaatggaaa cttggcttct
     1321 tctattgttc atttgactga taatttagac ttgatccctg gcacgtttga tttgatgtta
     1381 ctgccaaaat taactcgctc atggactttt gaaaatgaaa gtagattgct tgctactctt
     1441 ttagcacctt taaaaagtga ctatgatctc attattattg atactgtacc aacgccaagc
     1501 gtttatacaa ataatgcaat cgtggcgagt gattacgtta tgatcccttt acaagcagaa
     1561 gaagaaagta caaacaacat tcaaaactat atttcctatt tgattgattt acaagaacaa
     1621 tttaaccctg gactagatat gatcggtttt gttccttatt tagttgatac ggacagcgca
     1681 acgataaaat caaacctgga agaactgtac aagcaacata aagaagataa cttggttttc
     1741 caaaatatta tcaagcgaag taataaagta agtacctggt ctaaaaatgg aattacagaa
     1801 cacaaaggct atgacaaaaa agttttatcc atgtatgaga acgtattttt tgaaatgctt
     1861 gagcgaatta ttcaattaga aaacgaaaaa gaatagaatc acaaatcaca agtgattaat
     1921 cacaaatcac ttgtgatttg tgattgttga tgataaaata agaataagaa gaaatagaaa
     1981 gaagtgagtg attgtgggaa atttaggcgc acaaaaagca aaacgaaatg atacaccaat
     2041 cagtgcaaaa aaagatataa tgggagataa gacggttcgt gttcgtgctg acttacacca
     2101 tattataaaa attgaaacag caaaaaatgg cggaaacgta aaagaagtta tggatcaagc
     2161 cttagaagaa tatatacgga aatatttacc tgataaactt taaaaaggag tgaaaatgaa
     2221 atggcagtta cgtatgaaaa aacatttgaa atagagatca ttaacgaatt atcggcaagc
     2281 gtttataatc gagtattaaa ctatgttttg aaccatgaac tagatactaa aaatactcgt
     2341 ttactagaag tgaatctttt aaatcaatta gaagtggcac aagaagttga tttatttcaa
     2401 caaccatttg aagaattaca agctattcat gagtattggc ggtcaatgaa tcaatattca
     2461 aaacaaattt tgacaaaaga gaaagtggct taacatggcg aatattactg acttcaccga
     2521 aaagcaattt gaagatcgtt tagaaaagaa tgttgaacga ctaactaaaa atagactagc
     2581 ggttgaatcg ccaaccgctt ttttacttgg tgggcaacca ggatcaggta aaactagttt
     2641 gcgatcggca atttccgaag agacacaagg aaatgttgtt atcattgata atgatacgtt
     2701 caaacaacag caccctaatt ttgatgaact agtgaaactt tatgaaaaag acgtagtaaa
     2761 atacgttacc ccttattcta atcgcatgac agaagcgatc ataagccgtt tgagagataa
     2821 agggtataat ttagtgattg aaggtacagg acgaacaaca gacgttccta ttcaaaccgc
     2881 aacaatgctt caagccaaag attatgaaac aaaaatgtat gtcatggcag tacctaaaat
     2941 caactcatat ttaggaacaa ttgaacggta tgaaactatg tatgcagatg atccaatgac
     3001 agccagggca acaccaaaac aagcgcatga tattgttgtc aaaaacttac cgaccaattt
     3061 agaaaccctt cataaaacgg gcttatttag cgatataagg ctttacaata gagaaggagt
     3121 gaaactctat tcaagcttag aaacgccttc cattagtccg aaagagacct tagaaagaga
     3181 attaaatcgt aaagtatcag ggaaagaaat tcaaccgact ttggagagaa tagagcaaaa
     3241 aatggttcaa aatcaacacc aagaaacccc tgaatttaaa gcaattcaac aaaaaatgga
     3301 aagcttgcag ccacctacac caccaatacc caaaacacct aaacttccag ggatttaaac
     3361 cttaaaaaag taaaagagta gttaccaaaa aacggtaact actctttttt tataaaaaca
     3421 tttcctcatt ttttaaaagc tgatcgagaa ataaagcaca caactgtatt ctttcttttt
     3481 tcagttggta cattttgcag tctgtaacgt gccatttact gtttgttacc ttaacatatc
     3541 cagtcgaaaa cacaagggta tataaacgag caagctcgcc cttgtgtttt ctttgtgtct
     3601 atgttgcgtt ctcgtcacag caaacgacac gaacacactg gcgaaaatga accaacaaaa
     3661 cgaaaaaaga aaggagcgta cactgtaatt tatttctagc ttttaaaaca tgaggaaaac
     3721 agcttcactt cctcaaacgc ctaacaaaaa tggttgtcaa aaaactaaaa atcaaaaaaa
     3781 gaaaagagga aaagaaaatg gaaaaaacaa aaaaattaca attagaagat tttacagaaa
     3841 atgg
//
LOCUS       DAFCHQ010000153         3741 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000153 DAFCHQ010000000
VERSION     DAFCHQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.158"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..887
                     /locus_tag="LNC71_002815"
     CDS             <1..887
                     /locus_tag="LNC71_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBK6467816.1"
                     /translation="IGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVIIHLH
                     GSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENSIVV
                     PEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDEEIS
                     LKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPV
                     IATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFN
                     DGNFFDRILNLYQKGSKE"
     gene            1455..2516
                     /locus_tag="LNC71_002816"
     CDS             1455..2516
                     /locus_tag="LNC71_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBK6467817.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            2834..3424
                     /locus_tag="LNC71_002817"
     CDS             2834..3424
                     /locus_tag="LNC71_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBK6467818.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
BASE COUNT         1381 a          400 c          731 g         1229 t
ORIGIN      
        1 ttataggtat ctctttattg agtaaaccta atattattca tatgcacatg tcgtacagag
       61 ggagttttag taggaaatat gtgctgaata agtggtttag attattaaat attcctgtaa
      121 tcatccatct tcatggatcg gaatttaaaa aatggtatga tagtgtttct gtaaaaaaac
      181 aaaatcagat tcgcttgctg ttaagagagt ctgattccac gattgtactt ggagaaaaat
      241 ggaagaagat aattaaagaa attgagccaa attgtaaaac tgttgttgta gaaaattcta
      301 ttgtagttcc agaatatacc ataaagtgga attcaaaaat gtgccgattt ctgtttatgg
      361 gagttttgat aaagcgaaaa ggtgtacacg atttggtaga agcagttaaa aacattgcag
      421 aacaatatcc agaaaaaaag tttatttttg agattgctgg agcaggtgat gaagaaatat
      481 cgctcaaaga attgatagaa gaatataatt tgcaatcgta ttttgaattt acaggttggg
      541 ttgtgggaga aaaaaaggag gagtgctata gaagatcaaa tgtgatgatt ttaccgtcct
      601 ataacgaggg attgcctata tctattctag aggcgatttc ttatggaatg cctgtaattg
      661 ctacagatgt aggagatatt tcttcagcag tacgtgatgg agaaaatgga tatttgtttc
      721 aacctggtga cgtatcagcg ttgacatctg caatactaaa aatcaatgat aaagataaat
      781 acgtaaaatt ttcacgttgt tcgaaggatt tggcaataac gaaatttaac gatggaaatt
      841 tctttgatag aattttaaat ctgtatcaaa aagggagcaa ggagtgatga caaggaaaat
      901 aaaaaatgtt tcgattatgt aatgaactta ttaagtgaat aaaatagaga gaaaaaatat
      961 tgaaatagac gatgtaaatt tttatccgtg taagaattca aatgaatagt agtgttttag
     1021 tgcttgtaat agagcaagag tacaagcacg gtacattata taaaaacatt aatgatattt
     1081 ctataaataa atagattttg tattctggat gatgaagagg aagatattat ctgataactt
     1141 accctaaact ttgcaggggg tggttataag ttgtgtatat gtggcagtaa tgaatgagtg
     1201 aaataatcat attaagaaat aaaattgcaa tattgtgtaa tacaaaagta tattttcatt
     1261 attccaaggg aaagcatact tacgaggatc atattattga tttaataaat aatttaaagg
     1321 aaataatttt tctagaagaa gatagtatgt cattcacatc atcaagatca gagtctattt
     1381 ttttcagaga atttaagaaa atcgttgatg ccgtcattga ctaattgaga aaacgtgagt
     1441 gaaaggatag aaggatgaat tcgagtgatc taattagtgt aatcatacct gtatataatg
     1501 ttgaaaagta tgtaggaagt tgtttggaat cagtgtgtaa tcaaacatat aagaacctag
     1561 atattataat tgtggatgat ggatctaagg attcatctaa taaaatatgt gaagactatg
     1621 cattaaagga ttcaaggatt cgtattattc ataaaaataa tgctggcttg ggattggcaa
     1681 gaaatacagg acttgaacat gtaaaaggaa aatatgttgc ttttattgat tctgatgatt
     1741 ttgttgcagc taattatatt gaagcaatgt taaatggtat aaaaaggagt aatgcagaca
     1801 catgttatgc tggatattat gaatattatg atgataataa tattatttca aagccagcaa
     1861 gatatgatag tacgatattt acaaataacg ctataattga aaaagtgttg cttggaatga
     1921 ttggcactga gccgagttat aaagtcgatt ttatattgcc aatgtcagtt tggcatgcgc
     1981 tgtattcatc tgagataatt caacagcaca atatcaagtt tccgtcagaa agggattata
     2041 tatctgaaga tatcatattc catatttcat atttgcaaca ttcaaaaata acaagtttta
     2101 ttaaagattg tatctattat tatagatgta ataataataa ttcattgaca cataatttta
     2161 gagataatga gttcgattgt aatattgaat tagttaaaaa aattgaaggc gatttaagct
     2221 atatattaga aaatgagata tttgtaaaca aaacaaggag acttttgttg ggacgctgtc
     2281 gtacttgtat tcaaaaggca gttagatatg ctagttttaa taaagaattt gatttaaact
     2341 cctatttgtc taagatactt atgaacgatg agttgattgc ctgtattcac tcatacccat
     2401 ataaacaaaa tccaattaaa caacgaatat ttaactattt tttactaaac caatccatta
     2461 ttggggttaa aacattagca tatttaagcg gaatgggtag aaataggaga ttttaatcat
     2521 aaaaaactgt actatagtga atttttggag atttatattc cagttatata ttaatgattg
     2581 tgatacttgt actattgtaa aaattcgtta ggtttaaata actggaaata tcggaaaatg
     2641 ggcattatac atcacggaca tcccttcttt tggaaaaaat gttacgattt ttataagtaa
     2701 tattgtatcc ggtggaatca acgatgaaga taacatatcc atcgttgtag attcagtagc
     2761 attgaatgat gctcttgttg caagaaatcc gaataaaatt gttaagtata aattgaatta
     2821 ataggagata tgaatggaaa ttagcagtaa agaatattta aaagcggttc ttcgtatgga
     2881 aaagaatcta tatatagaat ctaaaggaca acacacaaaa ttactaaaaa taatgaaatc
     2941 acatccggac tacaacgctt ggcggtatac tcgtcgaatg agaattacat cttattatta
     3001 ttcaaagaga aagaaaaata tcatttatgc gattatgtat attgtgtctt gcagaagaat
     3061 gaatagtttg ggaagaaaat gtggtataga gtgtggtgaa aatgtatttg atgaaggtgt
     3121 aagattttac catacacaag gaattgttat taatggtaac gccataatag gaaaaaattg
     3181 ttacttatat gggaataact gtattggaaa tgatggaatc gatcctaagt gtcctgtaat
     3241 tggaaataat gtacgtgtat gcgtgggtgc aaagattatt ggtaatgtaa aaatagctaa
     3301 caatgtagtt gttgcggcag gagctattgt tatcaaagat tgtctagaga atggtgcaat
     3361 tcttgcaggt gtaccggcaa aaataatagg gtatgttaat ttaacaaaag tatttttaga
     3421 ataattttta gcgaaagaag gtgaataacg atgcgtattt caataaaaag atggtcagga
     3481 gcagattgga cattgttcac gttggcattt tttgcaatct ccatagttct tgtatatact
     3541 tttaacgccc caagagcaat tttatatttg ggagatataa ttaatatctt tgtgttatta
     3601 aatgcactta gctttagacg cagaaaagga atatatgcta gcaaaaatga gatgctgata
     3661 gttttgtttt tactagtttc tatttgtagc tcagttatta atttagaaaa tataggattg
     3721 attatctggg ggatacgaaa t
//
LOCUS       DAFCHQ010000154         3602 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000154 DAFCHQ010000000
VERSION     DAFCHQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.160"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..186
                     /locus_tag="LNC71_002818"
     CDS             <1..186
                     /locus_tag="LNC71_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6467819.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="LNC71_002819"
     CDS             329..553
                     /locus_tag="LNC71_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467820.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="LNC71_002820"
     CDS             568..1437
                     /locus_tag="LNC71_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK6467821.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="LNC71_002821"
     CDS             1418..2152
                     /locus_tag="LNC71_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6467822.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="LNC71_002822"
     CDS             2185..3093
                     /locus_tag="LNC71_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBK6467823.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="LNC71_002823"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="LNC71_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAFCHQ010000155         3492 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000155 DAFCHQ010000000
VERSION     DAFCHQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.161"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(228..1175)
                     /locus_tag="LNC71_002824"
     CDS             complement(228..1175)
                     /locus_tag="LNC71_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBK6467824.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
     gene            complement(1180..1932)
                     /locus_tag="LNC71_002825"
     CDS             complement(1180..1932)
                     /locus_tag="LNC71_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBK6467825.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            complement(1933..2841)
                     /locus_tag="LNC71_002826"
     CDS             complement(1933..2841)
                     /locus_tag="LNC71_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBK6467826.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(2841..>3492)
                     /locus_tag="LNC71_002827"
     CDS             complement(2841..>3492)
                     /locus_tag="LNC71_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBK6467827.1"
                     /translation="DRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHP
                     WEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTL
                     IIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQL
                     YDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT         1152 a          651 c          431 g         1258 t
ORIGIN      
        1 aatttcaaac tacgaattat gattcgtcat ggcaaaacat ttttaaccaa ataaacagaa
       61 cagaaagagg ttttcttctt tctgttctgt ccaaattatt ctaagatttc tctttaccaa
      121 catcatttga caaagagccc ttctatccca gatttgattt tacaaaactc atcagtcctt
      181 attgacaaag agcctgaatt taccctctaa tcttatctta gtatgtttta attgatttca
      241 gttttcaaat aattagtcat tccacttaaa ataccatatt tcggttgata gccaattgac
      301 tggatcttag taatatctgc tagagagtca tggatatcac ccgaacgctc ttctttgtat
      361 tcaagtgtca attctacacc gagaatttca ttgattgcat gaatcaattc caaaagtgtg
      421 gttgctttac ccgttccaac attgaattgt ttccccagtg cagcttcctc attcgcaact
      481 aaaagtaatg cttggattac atcatctata taaacaaagt ctctagactg gcttccatca
      541 ccaaataatg taaatgaagt tgcttctcct acgatttgtt tcttatagcg atctacgaga
      601 atagaaatca caccactgta tggagaattc ggattttgat ttggaccata gacattgaaa
      661 aaacgaactg cgctaccggg tacatcatat aaatgacaat aatctagcac atattgttct
      721 gcagcgaatt tatcaaccgc ataaggcgtc aatgggcgaa tcacggactc ttcttttttt
      781 ggtaaagtcg gttcatcccc ataaacagcc gctgaagaac taaacacgat ccgttttaaa
      841 tcaggttgat acttgcggat caattctaaa atcattaata cactttcaaa attcacacga
      901 tgtgtttcta ctggtcgggc aactgaatct gccacactag caactgctgc taaatgaaag
      961 atataatcaa atttattagc tttcatgatc tcttccatca aggatttctc agcgacatct
     1021 ccctcaataa aagtgatatt tttcgtcata tcaagatttt ctacttttcc cattgataag
     1081 tcatcgacga cgattaccac atgttcttct cctaaatgat tggctaaggt agaaccaatg
     1141 aagccagccc caccggtgat aagataacag ctcatccttt tacctccgat ataaataatt
     1201 atagatatct tgaagctccg aagaatcttc taaaaattta ggaaacaaaa attctaacca
     1261 tttaaaatct tcttcactat caatatctaa aattagataa tctctcattt ttattatccc
     1321 acattgccca tcaaaaatat aattactttt ttttagaaat tcaggtttaa aagaatacat
     1381 tgaggcattc atttcaaaaa cttcaggtgt ctgttgccta gatgtgtatc ttgatttatt
     1441 aactatagta acttcatttt caattctttt taccatatta aaatagggat tacgtcttgc
     1501 agaaacaact gaaaagtaga cgtctttatc taaatttttt agatgttcat ctattaattc
     1561 ttcaagatta gaaacttcac gtaaaggtga agtcaaatct aaatctataa ttaaatcaaa
     1621 tatattatct tctctttcta gttgtaagaa tgaatcacgt atcacttcaa ttttccctac
     1681 agtatctgta gctaattcag gtttacgttc tacaattttc acagacggat ttaacttagt
     1741 cgtcaattct actaactcat tactgtccgt attcaaacaa gtcgtgcaat tatgttcagg
     1801 atgtttttgt tgatagatat ctatcattgc taaagcgaaa tggactaggg gctttcctgc
     1861 caaatatttc aaatttttat ttttaaaccc cttagaccca gctctaccac aaatagtaaa
     1921 taaaatttcc atttatttct cctcagctat tttcaatact tcgtttgcat gatataaatc
     1981 attccagttt tctttgcctg ttactacgtt ttcaaagaaa tatttcaact ccttctcata
     2041 catatcatta gcagattcag gcatttcttt aacaatgttt tcttcaaaac ttattttatt
     2101 atttaataag tcaacattga tacattcatc taaagtaaac aattgaaatt ttcttgtagg
     2161 aactcttcca aaataatcta aatgaatttc tataatttta tcatcgtatc tcaatatata
     2221 tgttgctaaa tcattactgt taatttcaag attcgaatat ttaccaaact gtttttttac
     2281 tgatttaggg aaaccaaata ggtttataat ataatccaat tcatgtatac agtctaattc
     2341 tactcctccc cccatagatt taaaggcaga ataattattt ctataatcag taccttttct
     2401 ccaatcagga agataagtag aacaaatagc tcttacagaa tatacttttt tactttgaat
     2461 actattacga acaacttcca tgatattttt atacctcagt ggagcagcaa cataaatatt
     2521 tttgtaatca taattatatt gagtaagatc aatactctca gaaaaagcag gtttttctaa
     2581 aaatatgtaa tcaacttttt caacaattac atttagtgtt tccagatgct taaaagtcgg
     2641 atttgtaata aaagctatat cataatgtgt atccaaatcg tctctatttt gaatatacgt
     2701 tatttcttca aactcttttt ttccactgct actataagct gttatatcaa aattaataga
     2761 attctttttt ccatagctta ttagattttt tagatgtctc tttccaattg accctaaccc
     2821 aaaaaaaatt actttcatca ttaatttacc tcaatgtcgt taattaagtt aataatatac
     2881 ttatcttttt caaaagtata ttggggttcc attttctttt ccaaaagcgt gaaaaattct
     2941 ttaatctcct ctaaatatgc atcttcaata atagtatgtg aataattatt atcatgcata
     3001 acctcatcgt acaattgaat atctgtcata ctagaagttt cttcattcca tgaaaataga
     3061 gaatccgggt taccttccca ttctattaaa atcttttcac caattatagt taaatttctt
     3121 ttagctactc ttgaaacaac atctacactt aaagttccaa ctataccagt ttcatgctca
     3181 ataataagag tgaaactgtc cgaataatca atatcaagat tggataattt ctgggtcatt
     3241 acagtaaacg atttaacttt cccaaatgta tctataatcc aaggtaattc aatagcgaat
     3301 atttctctac acccattagt ttttttgttt gaaacaaaaa aattgcgata atcttcccaa
     3361 ggatgccagt cgggtaaata ctgtcctaca tggtattgat aagaaatttt tttattatgc
     3421 gtcactttag acttaatata ttgaatttct tttctcttca aaaatgtaga agatagataa
     3481 agtaatctat ct
//
LOCUS       DAFCHQ010000156         3448 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000156 DAFCHQ010000000
VERSION     DAFCHQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.162"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="LNC71_002828"
     CDS             complement(<1..2232)
                     /locus_tag="LNC71_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467828.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="LNC71_002829"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="LNC71_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          964 a          762 c          548 g         1174 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaa
//
LOCUS       DAFCHQ010000157         3239 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000157 DAFCHQ010000000
VERSION     DAFCHQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.164"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(554..2167)
                     /locus_tag="LNC71_002830"
     CDS             complement(554..2167)
                     /locus_tag="LNC71_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBK6467829.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="LNC71_002831"
     CDS             complement(2308..3216)
                     /locus_tag="LNC71_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467830.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1043 a          556 c          572 g         1068 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaa
//
LOCUS       DAFCHQ010000158         3200 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000158 DAFCHQ010000000
VERSION     DAFCHQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.165"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="LNC71_002832"
     CDS             301..585
                     /locus_tag="LNC71_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467831.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="LNC71_002833"
     CDS             588..1004
                     /locus_tag="LNC71_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467832.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="LNC71_002834"
     CDS             complement(1141..1860)
                     /locus_tag="LNC71_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBK6467833.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="LNC71_002835"
     CDS             2513..2608
                     /locus_tag="LNC71_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBK6467834.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..3156)
                     /locus_tag="LNC71_002836"
     CDS             complement(2911..3156)
                     /locus_tag="LNC71_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467835.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAFCHQ010000159         3147 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000159 DAFCHQ010000000
VERSION     DAFCHQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.166"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..875
                     /locus_tag="LNC71_002837"
     CDS             6..875
                     /locus_tag="LNC71_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6467836.1"
                     /translation="MGRAIQTIYNVVNKFKQGKTALDYWHQYKENKKKCGRKVIQLPA
                     HEVDYIKEKVTLGWTPDVIIGRKERSVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNG
                     HQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIIT
                     IKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPG
                     TPSQRPLNENSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIF
                     LSYVQEAFYSSLI"
     gene            complement(1741..2271)
                     /locus_tag="LNC71_002838"
     CDS             complement(1741..2271)
                     /locus_tag="LNC71_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBK6467837.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(2268..2885)
                     /locus_tag="LNC71_002839"
     CDS             complement(2268..2885)
                     /locus_tag="LNC71_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBK6467838.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT         1046 a          596 c          401 g         1104 t
ORIGIN      
        1 accgaatggg acgtgctata caaactattt ataatgtagt caataagttc aagcaaggca
       61 agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt ggtagaaaag
      121 tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact cttggttgga
      181 cgcctgacgt cattatcggg cgaaaagaaa ggtctgtttc atgcggtatg agaacacttt
      241 atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa ggtaaaagaa
      301 aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca atccatgatc
      361 gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaaggt gatacgatcg
      421 ttggcattca tcataaaagt gccgtcatta ctttagttga aagattatct aaagtcatta
      481 tcacgattaa acccaacggc cgtaaggcat tagatattga aactgccctt aatcaatggt
      541 tttctcgctt ccctaaaaac ttctttaaat ctattacgtt tgactgtgga aaagaatttt
      601 ctaactggaa agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa
      661 caccttctca acgcccatta aacgaaaatt ctaacgggat tctgcgtcgt aatggactgc
      721 cgaaatcaat agattttaga gaagtgaatc agacatttat ttccagtgtc agcaatcaac
      781 gcaatcatat tccaagaaaa tcattgaatt acagaacacc aatcgaaata tttttgagct
      841 atgtacaaga agcattttat tctagcttaa tttgacaaat catataattt aaaattaaca
      901 ttgattctcc tatgaaaata tcttttacct ttctcctgtg aaaaggaaat ttcctttttt
      961 atagtgttat tgatataatc agaatattca tcaaactctg tatctggagt taaaaatcta
     1021 cagtaacatc tataagcttt gctagtaatc ttcttgggtt ctctctccat tgtcaagcaa
     1081 catcaaaaca cctacttact attcgattct tttctgccaa aaacgagtca gcatcactca
     1141 caacttatgt gttgctgact ccgactagtc ctttgttcaa tatcaactta ttaaacagat
     1201 ataatatttt tctttatttc gtatactcca agacttccat ctttctttca tcttatctct
     1261 gaaaaaaaca cagcaaagtg cccttaataa atatacatta gaatttgcaa attttttcat
     1321 cttaagatat atcctattca tattctttac aatcctaatg ggtgattaca aatctattta
     1381 gttcaagttc tacattataa acacctttct tgatagtatc atctgctgca caagcgatgt
     1441 cgatccactt attaggggat ttacgtgctc aaaagtggct attccttttg ggaagtccat
     1501 ccataataat ggtccatgga tttttttagg gtcttgtcgt tcaaatttct caaaaacttt
     1561 attatacttc catagtttta taattatttg gtttcacttt ggctcgttca aacaccaact
     1621 tgtatttctt ttcaacgttc gaactggagc cccccctata atctcattat tattttcatg
     1681 actttttact accgtggaat tacaagcaac aacaatatta tctgctaaat gaacaccttt
     1741 ctatatcacg ctttttgctc caatccaaca atgtttacct ataactattt tattattttt
     1801 attcaaaatc ctatgagtat tcaattcaaa aatcgtatgt gaatcggtat ccataaataa
     1861 tgtatcccac gatactgtac agccatcttt gaattgaatt tccttaaagc aaattatact
     1921 catttttcct gtcgaattaa actttgaccc aatatctaaa actgcatttt tccttatgtc
     1981 aatatgagcc ccttgtgcga taaacgctgg cccttgtatt cttagtattc cttcaatact
     2041 tataatcgtt tcttcatttc ccgcagtatt taatactgga aagcccaaat aaactatttt
     2101 tccagattgt aatactatct caattttccc tctcgatttg cgtgtaatcc gtactttacg
     2161 atgcaccaaa ataggtaatc tccacgcatc tgaaatatga agaaatttat aattgaagta
     2221 tatcgtttta ggtaaattta atatattctt tattatcaat ccgagtctca tctttcacca
     2281 tctctttttg taatcaaaat tagtttataa aggattacac ccaatatacc gcccaacgta
     2341 ttatatacaa aatccgaaat ttgaaacgtt cctagcctca aaaaaagttg taaaaattca
     2401 attattgctg aaaaataaaa tgcaattttt cctccatata ctattgagga ccatattttg
     2461 ggtttcttta tattttcata tttaatagac attaccatat aaacatatgg aataaaaaga
     2521 ataatatttt ctaaacacgc tgtagataac tcttttgttc cagttgttgt attatactta
     2581 taaatccacc agtctcccat aacatcagat aaaggattta accaaattgt tcgattgatt
     2641 aatgttgtaa ataaaatcat cgcagtatag aatatcaaag cacaaacttt tctatatttt
     2701 ctatccttct ttaaatgaag ataaagttcc attagtatac gtttatagcc ctcacctgca
     2761 gcaatgccat ctgttgcata cataaaatag accagtccta aaattgtcaa taaaacagca
     2821 aacccaaagg gttcatataa tttatataat atatctgtaa taatttctgc aatcactatt
     2881 atcataatta tttctccaat atcaagtata cacttatcgc ttccaattac acatgatcat
     2941 attagagtgt attatcacat tctctaatat tcaatttatt tttcctaaaa tattcgaaat
     3001 aatcgtcttg ataacgagta agtattttta tacgattgat aacatagtaa caatattttt
     3061 aattatagta aaaaatcatc tgcctttttc atccacctaa tcttattttt cattttattc
     3121 aacatcctta aataaacttc cgttcct
//
LOCUS       DAFCHQ010000160         3101 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000160 DAFCHQ010000000
VERSION     DAFCHQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.167"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            775..>3101
                     /gene="esp"
                     /locus_tag="LNC71_002840"
     CDS             775..>3101
                     /gene="esp"
                     /locus_tag="LNC71_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBK6467839.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1133 a          460 c          602 g          906 t
ORIGIN      
        1 tattcttttt gatacatgat gaatttatgg attatcaaaa attggatcag ttaattgaaa
       61 gagaaagctg ggaagattat tacgtgggtc taactttttc agaaaagtta aacaactaca
      121 agttaataga ttataagtat ttgagtgaaa gcaattaatt attcatgtag agtactagac
      181 actttaaata tggacaattc aattataaag aaaaagaatc aataatttaa tgaggctgtg
      241 acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa atgttgataa ctccaatcat
      301 tcgtttaaga ttctaggaat cattcagaaa tgctgtgaaa ccgatacttt ataattataa
      361 agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca cctttttact tatgtcacag
      421 tctcattcag taatgcggga taaggctcct tattccgtta tttgaatact ctgaagttta
      481 atctaagaaa gggtaatatt ttgaaaacat gcttcatcgc atagaaatga acttaatttc
      541 agaaagaata catacatatc tttttatatg ttttcagctg ctgaatggtt aaaaagcaca
      601 aatatccgaa aaaaacatga agaaatgtac tttttttgtt gtaatgttca tgaaattctc
      661 taagtaaatt agttaacata aggttagtaa gattcctatt ctgtgaataa tacgtatcag
      721 atttatcttt tattcttact aggaaagtaa ttattaaggg agttgatttg aataatggtt
      781 agcaagaata ataagagagt atttctagaa aaaacaaaga aacgagtatt gaagtacagt
      841 atcaaaaaat taagtgttgg ggttgcatct gttttgattg gtgttggtct tgtcttggga
      901 acaactgaac tagttaaagc acaagatgaa atttctccaa gcagcccact tgaaacagcg
      961 ataagctccg tacaagtagg tgacaaagta gcttcgggga atacttttca ggagaatcct
     1021 ggttatacga aaaattataa cttttcggat ttacaattta gtcctcaaga actaactgga
     1081 gacacactaa aaggcaatac aattggattt gaagtttacg gaaaacataa tattgcagca
     1141 tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac gattagctaa atatgtagaa
     1201 aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta gacggacctt tgtaagaatt
     1261 aatgattcgc tgggtagacc tacaaatatt tggaaggtca attatattcg tgcaagtgat
     1321 ggattatttg ctggggcaga aacaaccgat acgcaaaccg ctcctaatgg ggtaattacg
     1381 tttgaaaaga gcctagatga aatttttaaa gagataggca tagacaatct caagaccgat
     1441 cgtttaatgt atcgaatcta cttggtaagt catcaagacg atgataaaat tgtaccgggt
     1501 attgatagta cgggctactt tttaacagat tcagatgatt tctataatag cttagatgta
     1561 tctgaaaata atcctgacca gtttaaacat ggatcagtaa gtgcaaaata tgagaaacct
     1621 aatactcaaa caaaagatgg ttcaggatcc acaggtgcta atggagctat aatcttagat
     1681 cataagttaa cgaaaaatta taatttctca tattcatctt ccgcaaaggg cacgccttgg
     1741 tatgctaatt acaaaattga tgaacgatta gttccatatg tagcgggtat acagatgcac
     1801 atggttcagg ctgacaaagt aacatatgat gtttcgtttg aatccggaac aaaagtagct
     1861 gatttagcaa tcgagcgacg ggaaggtcat gaaaattatg gcataggttc aataactaat
     1921 aatgatttaa ctaaacttat cgattttgct aatgcaagtc cacgtccagt cgttattcga
     1981 tatgttttac aattgactaa gccattagat gaaatcttag aagatatgaa agcaacagca
     2041 caagttgaag aaaataaacc atttggtgaa gatttcatct ttgattcttg gttgtcggat
     2101 acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt gcaagatatt
     2161 gatggtgatg gaaaccctga cgataaagaa gagagcggag acacgaatcc atatatcggg
     2221 aaacctgaat tagaagaagt atatgatgtt gacacaacag ttaaggggaa agtattcatc
     2281 cacgagttag cgggaacagg tcacaaagcc caacttgttg ataaagaagg tactgtatta
     2341 gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc caatttcaga tactgtagaa
     2401 tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt aaaaatccaa
     2461 atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttattaa ggaatcacca
     2521 aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa agaatcaatt
     2581 cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga gcctgatact
     2641 tctaacgtta ctgatagtac gaaaggtatt gtaactgtta agatcggaaa tcgaactttc
     2701 gacgtggatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga tgcaacatac
     2761 gtacctataa caacaactcc agaaacgaca gttcaaagtg gaaaacctac atttgataaa
     2821 ccagatgttc ctttagctaa agatgccttt tcaatttcag atgtctataa taagtacttc
     2881 ggtaatgcaa gtgttgatgc aaacactggt attgttacat tcactccagc taaaggtgta
     2941 ggagaatcgg agccgattac tggaacaatt cctattaaaa ttgtttacca agatggttct
     3001 gtaggcacga ccgatttagc agtaactgta agtaaagata tttatgaaaa tccaggagaa
     3061 aacattcctg caggctacca caaagtaacc ttcaccgcag g
//
LOCUS       DAFCHQ010000161         3072 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000161 DAFCHQ010000000
VERSION     DAFCHQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.168"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..1103
                     /locus_tag="LNC71_002841"
     CDS             288..1103
                     /locus_tag="LNC71_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467840.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1229..1486
                     /locus_tag="LNC71_002842"
     CDS             1229..1486
                     /locus_tag="LNC71_002842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467841.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1738..2310)
                     /locus_tag="LNC71_002843"
     CDS             complement(1738..2310)
                     /locus_tag="LNC71_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6467842.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2326..2931)
                     /locus_tag="LNC71_002844"
     CDS             complement(2326..2931)
                     /locus_tag="LNC71_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBK6467843.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1019 a          539 c          473 g         1041 t
ORIGIN      
        1 aaaataagaa attcaaaaaa gaaaaaactt tccttaaaaa caaaaaatag gagtattcag
       61 tttcttaata gttttgtaat agttgtatgt agaagttttc ttaatagttt ttgtaataat
      121 ttattaatcg tagtttatat aacttatttg taagtgacta gagaattaaa taaaaaataa
      181 ttactatcta gtacgaaatt aattacatag gataatatat taaggtgctg gactacaagt
      241 aaaaaatact gtatgatcat gacagattca agaaaggagc aataagattg gttaagatag
      301 caggactttc taaaggtgga aaaaccgttt cacaacttta taaacaaaac tacagagact
      361 ggagacagtt cagaaaaaac attaaggatt cttactttat tctaccaaca gaacttgaaa
      421 aatatttacc gaataacaaa gctattaatt tatatctgta ttactgtttt aaggcaaaga
      481 atgaaggcgg ggattcctgg cattcggtca ataccatagc agagtctcta ggagtgacaa
      541 ctaaaactat aaacacctgg aataaaacct tagttgattt aggtatgatt gcacgtatag
      601 acgataacca tctcagtaaa tcgacaattt tacttcctct ctctagttac tataaattag
      661 cccttgatag ttctttaaag gatgttaccg agagcactgt ccatgaaatt gatggcaact
      721 tggttgcagt ttatcatttg tttgaatgga gaaaaaatga ggctgatgaa aattataacg
      781 ttccttataa cacactctgt tgtgttttta aaagaaaaac acaaaaagat actatctata
      841 aatttatctt ggtccaagat gagaaaataa gtaatttcga attaagcact ccttctagta
      901 agatttatga cgatgcatat agatttgaat gtcaagatct agaagccctc ctttccgcaa
      961 ataatctaaa caatatagaa catgaaaact ttgttgtaaa cactagattt aatttagttt
     1021 ctgaaaagga ctcagatttg ttagatttgc tgatagagct tactaaaaat ctagataaga
     1081 aagaaaacat tactgtacta taactagcat cggtccatca ttaagtttgg actattagaa
     1141 cacttgctat tacagaaaaa ggtatgctag ccacgcttac tatttattgg tatcaaacaa
     1201 gtatcttaat ttgaaaggaa gatgatttat ggctaattta tatatagaga acaaaggaga
     1261 cttgaatgcc attattactt taatcgtagt tatggtaaaa tcaataggta atatcacaat
     1321 aggaatgctg tttattatat tagcagcagt gctgattttt atgatagtta tttggttctt
     1381 aaatgaaaga tctaaagaca atcaacaccg tagggatatg gagataaaat caatagagtc
     1441 aatagattat ttatcgataa taacactaaa aaagccacgt tcctaaacgt gacttacgct
     1501 gaacgatatg tgttgtttgg tagacgaaca aatcgttcat tataaatata tgatggtatc
     1561 ctaatatagt aagcgtggct agcatacctt taattacttt accttttaca gttaccatac
     1621 acattttttg agcagaaatc aagtaaataa aaaaatatta tatttcacaa catgctaata
     1681 gactcatatc tagaacgcat taagtattta cctgtctggt tcgctgagac ttctttttta
     1741 actttcttca attttcttct ttattctaaa aagcgtactg cgactgaaac cggttatagc
     1801 ttcaacttcc ttatacgatt tacctgaagt aagcagttca tatgcgtgcc tcttcttcgg
     1861 tgtgattgtt gctttcggac gtccttctct gaaattagga ttattctttc ttgcaaacat
     1921 ttttccttct tgggtacgtt ccacaatcat atcacgttcc atttctgcta cactcaacaa
     1981 cgtccgtgtc accattcgcc ccatggacgt attttcaatc gttcctagat tcaaaacctt
     2041 gatcgttacg ttatcatcga gtaaaggttc aatgatctct aatgcttctc tcgtgttacg
     2101 agcaaatcga tcaagcttcg taacaactat ttcatctcca ggcttcagac cagccaacag
     2161 ctgattgaat tttggccgat cccgttttgt acccgtaaat ttttctgaaa aaatattctt
     2221 cttctctacg ccgttctcaa gcaactgttg aatttgttct tttgtacttt gagaagcggt
     2281 tgagacacgt gcatatccat atttcgtcat ttggtccacc tacttttaca aatcatctat
     2341 cgtatactca gaataccctt catagaaata gctgacatcg attcctttca tatataactc
     2401 gcgatcgtca attttatcag tcagagcatt ggaaattaga tagcgtattt ccaaatcgtt
     2461 tatgggactt cgctccattg cagaaagata gtctgcttta tcgattaagt tccaatctac
     2521 aactttttgc aattcttcct ttaaaatgag atccagccaa attctagtac ttcggccatt
     2581 tccttctcga aatgggtgag cgatattcat ctccacatat ttcttgataa tctcttcaaa
     2641 agaagtttgt ggcatctgat caatatgctg taacgaatgt tctaaataca taactggtgc
     2701 aaatcgaaaa tttccttttg caatattgac cgatcggatt tttccggcaa aatcgtaaat
     2761 atccgaaaat agaaattcat gaatttcaga taaaccttta aatgtaccaa cttcgatttc
     2821 atttattttt ccacaatcgt acaattcttt cgctttttgt ttactgagtt tttcttcaac
     2881 cttagccaac tcaacttgat tggttaatcc caatttattc tctaaaatca tgcgaaatct
     2941 ccttaacttt atgacataag tttatgaaac actctcaacc ccattatatc gcgattttgt
     3001 ttatgtgtca aaaactatta agtttctgac ccccgataga tttttcaagt gtctctattt
     3061 tgcacagtct ag
//
LOCUS       DAFCHQ010000162         2648 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000162 DAFCHQ010000000
VERSION     DAFCHQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.169"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            15..338
                     /locus_tag="LNC71_002845"
     CDS             15..338
                     /locus_tag="LNC71_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467844.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            502..756
                     /locus_tag="LNC71_002846"
     CDS             502..756
                     /locus_tag="LNC71_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467845.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            826..1071
                     /locus_tag="LNC71_002847"
     CDS             826..1071
                     /locus_tag="LNC71_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467846.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1147..1512
                     /locus_tag="LNC71_002848"
     CDS             1147..1512
                     /locus_tag="LNC71_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467847.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1573..2136)
                     /locus_tag="LNC71_002849"
     CDS             complement(1573..2136)
                     /locus_tag="LNC71_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467848.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          776 a          472 c          419 g          981 t
ORIGIN      
        1 tttatttatt ctcaatgtta ggagtgatta ttttgccatt tattgcgatt ttattcgatt
       61 tagttgcggc agcagcttat tttctccaat taaattatca aacctcagga attctgttta
      121 ttggaatgat ttttcaaggt gtgattaccc ttttgcttct tgtcatgatg ctcagctata
      181 aaggaaaaag atatgcccgt attcaaacaa aagtatttgt taaatatgtt tccattcgct
      241 atgggatcat cattctttcc tttttagtga atgcgatcgt cctcttttta tatgtattga
      301 attacttaga tatcaatcca ttgatttttt ctcgatagat aagacacatt cagcggaaca
      361 gttcctgctt atcagcagaa aactgttctg ctttttcatt tttcattgat ctattcttct
      421 tttattttcc tagcatttca tttgtgatgt atgcaataac atgttatctt ttattatatg
      481 aatcgttgag gagtccgtta catgattacg ttggtgagag ttttgttttg ggtgcctgcg
      541 gtcgcactgg tcgcaagtat tgtttatctt atgaattgga acaaagaacg gttttattta
      601 gctattttga ctcttccggc tatctacttt atgtggaaag tttttaatta caactacttt
      661 gaacccgata gtgtttttat agaagaatta tcaggactgg ttttgtcttt attgatcgtc
      721 attctttatt tgatacgttt aaataaaaaa cattgagtac tattgattgc atgtgtagct
      781 ttctatagtc attataacta ttctactata atgaaaggtg attaaatgag tatcgaagaa
      841 gctattttac tggatacttt acgttgtcgt ctgaatttct taagaactgg ccgatcttta
      901 tctcaagtag ctgtttctag cgcgttaagt attcctttat tcaaatatcg taagtacgaa
      961 gaaggagaag aaattccaag catcattgaa ttaatctata tcgctgactt ttacgatatt
     1021 tccgttgatt acctaattgg tcgctcagaa acagcaagca aactgaagtg attttttcca
     1081 gccttggtgc tggttttttc atttaacgtc taaggttaac aatctatata taaaggagac
     1141 atttctatgc gattatggca tgaaaaattg atttcaaagc ttcctcgtca acagctttta
     1201 ggacaacatc gggaatgctg tgcattaaga ggaaatggct ggggaaagaa acatgctact
     1261 gtggattatg tttttcacta ttctccttat agattattcc aatatcatca gcttgtcatg
     1321 gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt ggaaaaatcc tatgtatcgt
     1381 ggcaaatcat gcgaagcatt ttttgattta cctactgtag agcgcaacgt gccgatttat
     1441 ccggaacacg atgaagttta tctagcagaa tgtttagcaa atttggagca aaaaggaatt
     1501 tatcttacct aatataacta gaaaagcctt ctatctcctc ttacttcatt aaaaagcttt
     1561 aaccatctta gatcaagcta aaatgtcgta agttttaggg ttatcttcat cctcacgtgc
     1621 aaaactaaaa gcgtactcta gttttggttc atcgccaact tccctagaca taaatgaaca
     1681 tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct gtatacgatg cacagcacag
     1741 attgcttctt ttgatctttc ttttgatttt tctgttgctt cttctacacc ttcttcgatt
     1801 actacatcga tgcttacaat tgcttctgcc taatattccc atgcatcgtc gatttaagcc
     1861 tctgtagtca ctgtgatcgt ttccacattt taactcctct gtcgtggctt cagttgattt
     1921 tgtgatcagt tcagttgcta agccagagtc agaacaacct gccattgcta gcgttaatat
     1981 tgatagtaaa aatagttttt ttatcataag gttccccccc tgaaaataga ctaaaataat
     2041 aacgctttct ttttttgttt tcagacgaaa attaataatt tctaaatatt ctttaaaaaa
     2101 gcttataact attatgcctt ttttaatata taacacagtt atttttaaaa ataagaatgc
     2161 taaatcactc tacaataacg gcgaaagcac aaaaaataaa aaaattatta cgttattaaa
     2221 aaaacagatg aaagatcagg ttttattaga tctcattctt tcatcacttg aagggattta
     2281 atgaactact ttttcctctt tgtcgttttt atatattcgt attccttcaa acagtaggat
     2341 agcctctttc aatgcttgat atttgaactg tccgttactt aaaaatttag atgtgccgcc
     2401 ctcttcctag aacatattct tttttacttt ttcattaatt gacagaccaa tcgaacgaaa
     2461 ctgtaaattt cgataggata atgattggtc aaactcattc attttcaaga catctcgatt
     2521 catattcaag ataggaatct ttttatcaac atcactttat tcgtattttc ctctaccata
     2581 aaagcttcac gctccacaaa attgttttat acactactat tcagcccatt caacgaccaa
     2641 tgaatttt
//
LOCUS       DAFCHQ010000163         2512 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000163 DAFCHQ010000000
VERSION     DAFCHQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.170"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            212..2365
                     /locus_tag="LNC71_002850"
     CDS             212..2365
                     /locus_tag="LNC71_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBK6467849.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          866 a          432 c          549 g          665 t
ORIGIN      
        1 cgacttaaga aaacataatt ttcaaaaata agtttatttt ttaatgaaaa aaatagttgg
       61 atatgtaact ttaagtgatt gctgtgaaac aaaaagttga ttttatcttg aaattaggta
      121 aagaatttct tgctccgccc attttttctt ttttattaag aagtttttgg gtacaatgaa
      181 gcatgaatgt aaataggcgg aaggatagga aatgatgaaa gattttatga aaaaaatatt
      241 aaatacttct tggttagaaa aaataagaaa aggtgcacaa gaacctaaga acaaaacgtt
      301 tcgccggtcc catgttcctt ttcgcttaaa ttttcttttt ttcattatat ttacgttatt
      361 tgttgcgttg attgctagat tgggttattt gcagatcgtg aacggcgaag agatggaggc
      421 gcgagtgaag tctacttcta cgattacggt ccaagaaagt actcctcgcg gaatgatcta
      481 cgactccaca ggaaaagctt tagttacgaa taaagcgaat caggccatca catatacgag
      541 aggaacacaa gttacggcta aagatttgct tgaaatcgca acgaagctga actcgctgat
      601 cgatgttccg gtagatgaaa atctgacaga tagagataaa aaagattttt ggctggctga
      661 tcccaaacat ttgaaagaag ccacaaaaag actttcggca aaagaaaagc aattagggaa
      721 cagcgaacaa tatagtgcga tggtcaataa agtcacagct gatgagatta atttaaacga
      781 ggagcaatta aaaattgcta ccatctttaa acggatgaat agtgcatatg aattaaacac
      841 tgtctatatc aaaaactctg gtgttactga tcaagagtta gctgttgtgg cagaacgcgc
      901 ttctgaactt ccaggagttt cgactggcac agactggaca cgagaatata atgcagcaag
      961 ttcactgaaa agtattcttg gcagtgtgac gactgaaaag caaggacttc cagcagatga
     1021 agcagagaaa tacctagcaa aaggttattc tcggaatgac cgtgtaggac aaagttattt
     1081 ggaaaaacaa tatgaagatg tcctgcaagg aaccaaaacc caatatgaag tttcccttga
     1141 taatgaagga aatgtttcga atcaaaaaga aatcttctct ggggaaaaag gttctaattt
     1201 aatgttatct atgaatgcag aattccaatc aaaagtagaa gaaattttga aaaggaatta
     1261 tcaaacattg atcaacaacg gcaaagcaca gtattcgcct ggtgcttatg cagtggctat
     1321 gaatcctcag acaggagaag tgctagcaat gactggtttc agtcatgaac aaggttccaa
     1381 agaaatcacc gagaatgctt taggcacgat tactagcgcc tttgatccag ggtctgtagt
     1441 caaggctggt actttgacag caggctgggc aagtaacgcg atcagcggaa atcaagtttt
     1501 gattgatgag ccgattcgtc tgcaaggtgc ttcagaaaaa agctctgtct ttaatcgaag
     1561 cggacaagtt gctcttgatg cagtcaaagc atcggaattg tcttctaata cgtatatgat
     1621 caaagtggcg ttaaagatgt taggactaga ctatacacct ggaatgggac tacctagttt
     1681 agatgaagaa gctaaagctt atcagcaatt gagagactct ttcaaagagt ttggtcttgg
     1741 tacaacgact gggatcgatt tgccgaatga atctcctggg atctcacgtt ctgttgatta
     1801 tatgaagaag ttcaatgcgg ataatggcaa agaatggtat gctccaggta actttaccga
     1861 tttggcattt ggacagtttg acacatatac accaatccaa ttagctcaat atgcttcaac
     1921 agtcgctaat ggcgggaaac gagtacagcc gcacctagtc aaagcgatct atggcaatga
     1981 cgaaaatgga aacttgggtg aagtgaagaa agaaattggc acaacagtag aaaatacggt
     2041 caatatttct gcagaaaata tggcgatcct ccgtgaagga ttccaccaag ttgtccatgg
     2101 taccgatcct tacacaacag ccaaaccatt agccagtgcg aaaatggacc tttccgcaaa
     2161 aacagggact gcagaaacgg ttgcggaagg tcatccagat attgcgacag tcaatagtaa
     2221 tatcgtggca tacggaccaa cagacaatcc ggaaattgcc atcagtgttg tcttgccgaa
     2281 cttattagac gaacaagacc acataaactt gacgattgct aaagaaataa tggacgcgta
     2341 ttatgatatg ttcatggcaa aataaaaaag aggtcgtgaa aaagccgctt agtttcgagt
     2401 atgaagaaaa attttgtgaa aatagcttct cacatttttg cttaaatttg acttcatacc
     2461 gagaaactgg cttttgaaca ccgtttactc ggcaaaaaag gttgtgacaa aa
//
LOCUS       DAFCHQ010000164         2459 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000164 DAFCHQ010000000
VERSION     DAFCHQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.171"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..1150
                     /locus_tag="LNC71_002851"
                     /pseudo
     CDS             6..1150
                     /locus_tag="LNC71_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="LNC71_002852"
     CDS             1321..1779
                     /locus_tag="LNC71_002852"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBK6467850.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="LNC71_002853"
     CDS             1938..2408
                     /locus_tag="LNC71_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBK6467851.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          460 g          716 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DAFCHQ010000165         2400 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000165 DAFCHQ010000000
VERSION     DAFCHQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.172"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="LNC71_002854"
     CDS             207..2069
                     /locus_tag="LNC71_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6467852.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="LNC71_002855"
     CDS             2069..>2400
                     /locus_tag="LNC71_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBK6467853.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAFCHQ010000166         2395 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000166 DAFCHQ010000000
VERSION     DAFCHQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.173"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            316..570
                     /locus_tag="LNC71_002856"
     CDS             316..570
                     /locus_tag="LNC71_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6467854.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="LNC71_002857"
     CDS             571..888
                     /locus_tag="LNC71_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBK6467855.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="LNC71_002858"
     CDS             1447..2382
                     /locus_tag="LNC71_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467856.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAFCHQ010000167         2377 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000167 DAFCHQ010000000
VERSION     DAFCHQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.174"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(334..735)
                     /locus_tag="LNC71_002859"
                     /pseudo
     CDS             complement(334..735)
                     /locus_tag="LNC71_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
     gene            complement(924..2243)
                     /locus_tag="LNC71_002860"
     CDS             complement(924..2243)
                     /locus_tag="LNC71_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBK6467857.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          739 a          407 c          452 g          779 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgatc
      721 cctgaataat tcatagcttt aattctttgg cacattttat tgaaatatgt atcactaata
      781 tccctatcaa ctgattttga ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg
      841 aggaataaat tttgggtgta aggcattttg agcatacttc tccctggata acttcccagt
      901 aaaaaaatcg ttagattgtt ggattagatc cattgactgg ctcgcctcag gcgagcaaag
      961 accctgtaga attcattctg atacacatga taactagata atttgagata gactcgtcta
     1021 cccgtttgga ctaatttccc agcaactttc agaaacttca atcgaataga atgaatggtc
     1081 attccctttt ggacttcttc aaagccaatc gttcttaaga agttgactaa gttgtaagct
     1141 atcaagctga gcatcattcg gacatgattc tccaaaaagc gaggactgtc tgttttgtca
     1201 aagtaaaagc cagctttcgc ttctttaatg aagttctcca ttgtgccacg tttggcatag
     1261 atagaaaaga tggtatcagg agaaacattt tctgatagat tcgtcacgat aaattcatgt
     1321 cgaaagagta gttcgcccgc ttcacgtgtt gagcgtatac acacgcgacg actttgtgac
     1381 caggattgtg cttgataagt ggtcgagaag tactgaactt ctcgttcttc ccacttttga
     1441 ttatcgccat aaagtactga tttctcagct atttgaccta gtctacgatt attcttcagt
     1501 cgaataacat aatgactctt ttttgattca cacgaatcat acacaccagg tgtagcgaac
     1561 ccgctgtctc cacgaaccaa gatgtcagtg tttggtagag agtgattata gtgctctaat
     1621 aaaggtgtga gaaactcctt tacgcctttt gatgtatatt gatttcctga acgtagttca
     1681 gcttttaaga agtcaccagt caatccgtca aaagctacca atggatggta accataggtt
     1741 tggtagtgtg tattataatt cgtttgttct tggtgaccaa atgtatctga atgggtcgaa
     1801 tctaaatcaa tgattaattc cgtatcatta cggatgaggc gtgctttatc gataagttct
     1861 tggttcaaag cttgaagttc atggatattc tcctcagata gtcgatctaa aaatcgggaa
     1921 agtgaagatt gagaagcgag ttctttccta tctaaaacag ctttaaacac aggatcttgt
     1981 ctcaggagat tagctgcaga atcagctgag taaccagcaa ttaattgcat aatgaactgc
     2041 tcaagtatgg ataagttgtc atgagtaaaa tacgctcgtt cgtcttcaat gtgaatgtgt
     2101 tgcttagcca gttcagaaaa accaaagatg ttcatcagct ctttcactag gacgagaccc
     2161 gaatcactcg ataattgacc acctgtatga gaaatagtga tatttgaatt gaattttact
     2221 tggtttttgt gtaagctagt catgaaaaga actcctttct tttggttggt ttagtcactt
     2281 taaccatagc agaaaggggt tcttttttca tcacttaacg ggtgaagatg aaaaagtaat
     2341 tgtaagctgc cgtgaggcag tttcacatgg ttttaat
//
LOCUS       DAFCHQ010000168         2331 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000168 DAFCHQ010000000
VERSION     DAFCHQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.175"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            888..2276
                     /gene="ltrA"
                     /locus_tag="LNC71_002861"
     CDS             888..2276
                     /gene="ltrA"
                     /locus_tag="LNC71_002861"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBK6467858.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          813 a          409 c          506 g          603 t
ORIGIN      
        1 gattttttat atattttagt ttacataata tatattatac aaagtagatt taaaaaatga
       61 taaacctctc tcgaacggtt ttttatcaaa aatcgtcatt tttccatcaa aatctgctta
      121 tgcaaaatta taaaaaaatt ttgcagtttt tcttactttt tttgtcgttt aattgaatga
      181 taaaaaccag tttctttatt cagttaatca aatatgaacg aattagatat gaatcattgt
      241 atttgccgtt gcggaaggat tttttaatta accacagata aacattgata acgattcgct
      301 tattgtaatc aacagattaa atttcatatg ttcatatgta cggatacatt ttcggaccga
      361 ttaattttaa ccattctatt tatttgctta cctattagat atcaataaaa aactgcttaa
      421 cagctatttc agctgttaag cagttttagg atgtgcgccc tgcatgggtg aaaacttggt
      481 ggtgaaagtc cactacaggc ttggcagtag gaactgttag cgaaagacaa ggcggcaatc
      541 gtgaggttgt ggctgaagga agtcggacgc aaactcctga actgacgaac agaaactaga
      601 tacaaggctg aattaggtcg gataagtctt catcacaaga caaagtccaa tactgcccga
      661 aacctataca gtaaatctag cagttacatg ggaggaaagt tttcactctt accgggggag
      721 atctcatgag ggtgttgtgg gcgttgaatg aaaacaaacc agcacaacga tcatggcgac
      781 atgatggtga atcatgagaa gtcagccgag gtcatagtag taacgacact gaccgttacg
      841 aaggactgaa caataagaat cttgaataat ttaggaggtg tgagaagatg cgacgaccgc
      901 agaaaacaag aaaacttggc tatcatcaaa gagctagtgt ggaacacgaa gggtatgatg
      961 aagtgcgcag cacttcttgc ggtgaacaga ggaaacaaga tggtgtagaa aatctgtttg
     1021 ggagaattct agatcggaat aatctcaatc aagcgtatct tcaagtagtc agaaataaag
     1081 gggcggctgg tgcagatggg atgaccgtcg atcaactgct tccttacttg aaagagcacc
     1141 gcgaggaatt actatccaat ttacgccaag ggaactataa acctcagcct gttttgcgag
     1201 tagaaatacc gaaaccaaac ggcggtgtaa gaaaactagg aattcccacg gttattgatc
     1261 gtatgcttca acaggcaatc aatcaagtgt tacaaccgct atttgaacca gaattctcag
     1321 ataatagtta tgggtttcgc ccaaatcgca gcgcacatga cgcagtgaag aaagcaaaat
     1381 cttattatga acaaggttac cgatatgtgg tggacattga tatgaaagcc tactttgaca
     1441 ccgtaaatca cgataagctg atgtatttca ttgagcggaa agtaaaggac aaacaagtcc
     1501 taaaactgat ccgtcaatac ttaatgagtg gtgtaatgga aaatggactc atcacaacaa
     1561 ctgaagaagg aacgccccaa ggaggcaatc tctctcccct gttaagtaac atctacctga
     1621 acgaatttga tcaagtgcta gaaaatctag gacataaatt tgttcgatac gccgatgact
     1681 gcaatatcta tgttagaagc aaacgagcag gacagcgtgt gatgaagaaa tccattcaat
     1741 ttctcgaaga acaactaaag ttgacggtaa atcgagaaaa gagtgcggta ggaagtccat
     1801 tgaaacgaaa gtttcttgga ttctgcattc tgccaacaaa gaaaggtatt aaaattcgcc
     1861 ctcacgcaaa agcaaaacag cgtgtgaaaa gcaagctcaa acaactcact aaaagaaacc
     1921 gtggaagaag tatccaactt attttaaaag aaatcaagca actaatgact ggttggatca
     1981 actactatgg aattggtgag atgaaagaat tcatgaggga gttaaacggt tggttgaagc
     2041 gaagaatcag acaatatatt tggaaacaat ggaagaatcc aagaaccaaa agaaaaaatc
     2101 tgattcgttt agggatcgat aaagcgaaag cgtatgaatg gtcaaataca agaaaaggta
     2161 tttggtcagt ctccaaaagt catattcttc atcgatcatt aacagacaaa gaactggccc
     2221 gccaaggata tgaggacatc tccttgaagt accagttcgt acactcaact tattgaaccg
     2281 ccgtatacgg gtccgtacgt acggtggtgt gagaggggcg gaaggtcagt t
//
LOCUS       DAFCHQ010000169         2278 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000169 DAFCHQ010000000
VERSION     DAFCHQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.176"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(146..445)
                     /locus_tag="LNC71_002862"
     CDS             complement(146..445)
                     /locus_tag="LNC71_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBK6467859.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            796..1428
                     /locus_tag="LNC71_002863"
     CDS             796..1428
                     /locus_tag="LNC71_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBK6467860.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1551..2189
                     /locus_tag="LNC71_002864"
     CDS             1551..2189
                     /locus_tag="LNC71_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBK6467861.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          765 a          362 c          331 g          820 t
ORIGIN      
        1 taattaaagg ataaaataca atataaagaa acatctgttt ctttatttag aataaaaaaa
       61 tacagactct gttcaaataa atgaacctag cctgcattat gagattgact atacttttta
      121 attcattgag atgaataact cgtttttata aatgcccaaa agtaatcggt gccgaagcca
      181 ttcctcgacc taattctttt tcaaattttc ctattgttga tatctccata taaaacggtg
      241 atactggttt agacaattct cccctgattg ctaacggacg taaactacgt tccaaattat
      301 ttatgacttt aggataatac tgaccttttt ctaagcctgt ctttgcgtct atcaaaagta
      361 tacgttcctt ttctttgatc tctggattta aaacgagatc atataatttt agtaccatgt
      421 tttctatttc ttcactttta gacatcacga tttcctccca aaaatatatt tcttcccact
      481 acattctata aacaaagtat acgctacatc tagatttttt taaatcatta ctggattgtt
      541 aaaacctttc aatacactgt tatggcaacg atttttattt taaaaacatt ctaaattaaa
      601 tcagtaaaaa tttttattct aagattttta ttagaattac gcctaatttg tgtctctcag
      661 ggcaattcta agaacgtttc aagagatagc gacactatcg ttatcgaaaa aggcgaaaac
      721 ctatctcatt ttctgctaat gtaagttggt ttagcttttg ttaatatgac tttatgaaaa
      781 tggaggtaat taaatatgat ttttcctaaa gttaatgaag caagcttgaa taaaggatcc
      841 ttttttcata tctatgaaaa cgaagatttt gaactgtata atatcctttc acaaaaagag
      901 gtcatcgaaa accaaataat ctgccatcca attatttaca aatctatttt caagtatgca
      961 ttttttgctt ccaatgattt tcaaagaatg gagattaccg actgtatatt cgaaaactgt
     1021 gatttttcag gctgtatatt gacaaaaagc atgttgcatc gcgtgaaatt tgtcaattgt
     1081 aaattaacag gtactaaatt gatggaaagt tatattggga acacgttatt cgagaattgt
     1141 aaaatggact atgtaaattt atctggttca aacataaaag aaagtacctt tgagcactct
     1201 gtcttatcaa gcgcagattt tgtagactgt tccctcacta aaataatgtt ttctactaat
     1261 gatttagaaa atgctagctt ttttgatact gatttaaaaa atatcgattt aagtagcaac
     1321 tcctttgaaa tgattgaagt acgctcggaa catctaaaaa attgtacgct taatcagttg
     1381 caagcacttg gttttgctag aaaattttta caaataaaag tagtataatc aaaatgaata
     1441 tataagataa atttttagca atacaaatat ccataaaaaa acattgccta ttttaactct
     1501 tcatgtatac tgactctatc ttcaaaaact gaatagtagg agttattatc atgaataaca
     1561 ttttaggaaa aaaaatagaa cttaaaaaag ggatattggg cactattgtc gttgtactcg
     1621 gtttgttagg gttgctattt gcgccatcca ttattagcat tttttcttta actatcactg
     1681 gatttatagc atttacactt gtttttggta aaaaagatac gaaaaaaatg ttctcgaaac
     1741 ctgtcgctcc tgtcaaaaat atagccaaat atttttttat caatgtagta atcagtgccg
     1801 cagtctcggt atttttgcaa caaattttga aatggggttt aacaggcaat ccaataaatg
     1861 aggctttcag cccgttatta tttattattt tgcctatcat gatacttgga gaagaattgt
     1921 tctcagtgta ttttctcgct attttttcta gtaagttcag cattcctgtc gctagtttct
     1981 tgtcagcaat catctttgga tttatccatt acagtactta tgataatgga aatattttac
     2041 acactgtagc acacatcctt ttgatacaag gggtggcaag attgttattc aaccaagctg
     2101 ccattaaaag caactcgatc attacaagtt gggcagttca tgtgatattt gatttcacag
     2161 ctatattgct agtcgtttta ttctcctaaa tttttataga aataatacat gctctattgc
     2221 taagaaagca tactttattt cctttaaaca aaaagacaga tgtatcaatg gattaaaa
//
LOCUS       DAFCHQ010000170         2227 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000170 DAFCHQ010000000
VERSION     DAFCHQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.177"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..280)
                     /locus_tag="LNC71_002865"
     CDS             complement(<1..280)
                     /locus_tag="LNC71_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBK6467862.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="LNC71_002866"
                     /pseudo
     CDS             742..2124
                     /locus_tag="LNC71_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          654 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaa
//
LOCUS       DAFCHQ010000171         2163 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000171 DAFCHQ010000000
VERSION     DAFCHQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.178"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..156
                     /locus_tag="LNC71_002867"
     CDS             <1..156
                     /locus_tag="LNC71_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBK6467863.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="LNC71_002868"
     CDS             169..1077
                     /locus_tag="LNC71_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK6467864.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1697
                     /locus_tag="LNC71_002869"
     CDS             1077..1697
                     /locus_tag="LNC71_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467865.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1701..2150
                     /locus_tag="LNC71_002870"
     CDS             1701..2150
                     /locus_tag="LNC71_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467866.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
BASE COUNT          761 a          331 c          494 g          577 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaga atacagaggt tatggaagcg actatagttg aaaatggtct tccctacgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgtaggtg ggaaatatcc agtccaatta
      361 gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaact atatttttga taaatattcc
      421 atgcagtgtt tacatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ttattttgtc attaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aatcgaagat ttaattgcgg atatgacgga ctttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgaacgttgg
      661 agacaagaac aggaacacag tttccaagat tggagagaag gacaagaatc agactatttg
      721 gcatggtttg aatcaatcaa ggatatttta aagacgattg atccaggtgg agtcatgtta
      781 gccgaactaa cggatgcgcg agtagatatt caaggagttc gtcatgcgtc tatttcagag
      841 cgtttgctgg cagatatgga ctatttatat cagaaattgc gagcaacgct tttcacgatt
      901 gaatacgggg agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcat
      961 gaagtcgaaa aagttggaac tgtagcattc ccgattgaag aaggagcctt gatcatcgca
     1021 accgttgatg atccaaagca aaatgtgttc actcttgaga aagttggggt gatctaatgg
     1081 ctaaaactaa acgaatgatg gaaacggatg aaaaaacggg tgtacaacgc caattctttc
     1141 caatcacaca tgcttccgca gttcttgggt tagaagaaat aatagcagga gaagcaacag
     1201 ttttatctgt taatggaaaa attggagccg tcgtcattac taaagaagat ttgggattag
     1261 agaatgttct cacagaatta ccctatgcaa gtgaagaaga tgacggcatt atcacggcag
     1321 aaatgtatca aaaaatttta aacagtggag aaggtgacta cgtgttgcca gttgccacta
     1381 tcgaccgttt gggtggcata aaagttggtg aattattgac gattgatgag acagggaaag
     1441 tctctgcagt cagacaatct gatgtcaatt tttcgctgga gttaaaagaa aaactcgatt
     1501 cactgaaaaa ttatactgct ggagaaaaca tcactattga tgaagatgga acaattagtt
     1561 ctacaggtgg tagtgggact ggtggtgtca atcagtcata tgtagatcaa aaattccaag
     1621 aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa
     1681 aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc aatgtacagg
     1741 tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg aagggagata
     1801 aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt ggttcaaccg
     1861 cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt ttaccaaagg
     1921 gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca actacagcaa
     1981 atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct ggagcacaga
     2041 aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaact gacaaagcta
     2101 aactcgatgg attagccaat attacatttg agaaagtggg gacggtttaa tggctgatat
     2161 tgt
//
LOCUS       DAFCHQ010000172         2160 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000172 DAFCHQ010000000
VERSION     DAFCHQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.179"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..156
                     /locus_tag="LNC71_002871"
     CDS             <1..156
                     /locus_tag="LNC71_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBK6467867.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="LNC71_002872"
     CDS             169..1077
                     /locus_tag="LNC71_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBK6467868.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1694
                     /locus_tag="LNC71_002873"
     CDS             1077..1694
                     /locus_tag="LNC71_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467869.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1698..2147
                     /locus_tag="LNC71_002874"
     CDS             1698..2147
                     /locus_tag="LNC71_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467870.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          776 a          308 c          470 g          606 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaaa acactgaagt tatggaagct acaattgtcg aaaatggtct tccatatgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgttggcg gaaaatatcc agtccaatta
      361 gaaacaagaa ttgtggatgc taaaaaaggg aaaatcaaat atatttttga taaatattcc
      421 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      661 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      721 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      781 gccgaattaa tggatgcacg tgtagatctt caaggtgtac gccatgaatc gatttctgaa
      841 cgtttcctat ccgatctaaa ttatttgtat caaaaaatga aagcagcact tttcacgatt
      901 gaatacggtg aaattgaagt gactgatatt cttcaagacg atctcttttc agataatcac
      961 gaagtcgaaa aaattgaaac agtagaattt ccaattgaag aaggagcttt gattattgca
     1021 actgttgatg atccaaaaca gaatgttttc actcttgaga aagttggggt gatttgatgg
     1081 ttaaagtaaa gagaatgatg gagactgatg aatctggtgt taaacgtcaa tttatgccaa
     1141 ttacacatgt ttctgcagtt ttaggcttag aaaaaatgat atcaggacaa tcaaaagttc
     1201 tttcggttaa tggtaaatat ggtgcagtca ttttaacgaa agctgattta ggattagaga
     1261 atgcaattac tgaactacct tatgcaagcg agacaagcga tggtattctt actgctgaaa
     1321 tgtttcaaaa gatcgtaaat ggagaaggtg gaacatatat tttgccgatt gctactcctg
     1381 aacaattagg aggtataaaa gttggggaac tcttagaagt tactgaagaa ggtgttttat
     1441 ctgcaactaa gcaaacagac ttcaatttct ctgaagaatt aaagggaaaa ttagaatctc
     1501 taaaaatatt aaaagcaggt gcaaatatat caattgcaga agatggaaca attagttcta
     1561 caggtggtag tgggactggt ggtgttaatc agtcatatgt agatcaaaaa ttccaagaag
     1621 ctgtaaatca agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa
     1681 tcggggaggt atagaaaatg acagatatcg taaaaataaa acaaagcggt gtacaggttt
     1741 atccacaaac tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag
     1801 gcgatcctgg ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt
     1861 ctgttacgaa tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag
     1921 ataaaggtga tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg
     1981 ggttgatgtc gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa
     2041 atccaggtaa tgctacaact acgacagcag gtttaatgtc agcaaccgat aaagtgaaac
     2101 ttgatggatt agcgaatatt acatttgaga aagtggggac ggtttaatgg ctgatattgt
//
LOCUS       DAFCHQ010000173         2058 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000173 DAFCHQ010000000
VERSION     DAFCHQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.180"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..914)
                     /locus_tag="LNC71_002875"
     CDS             complement(<1..914)
                     /locus_tag="LNC71_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBK6467871.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="LNC71_002876"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="LNC71_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBK6467872.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          685 a          369 c          401 g          603 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggca
//
LOCUS       DAFCHQ010000174         2019 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000174 DAFCHQ010000000
VERSION     DAFCHQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.181"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            100..570
                     /locus_tag="LNC71_002877"
     CDS             100..570
                     /locus_tag="LNC71_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBK6467873.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="LNC71_002878"
     CDS             792..1895
                     /locus_tag="LNC71_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBK6467874.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAFCHQ010000175         2003 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000175 DAFCHQ010000000
VERSION     DAFCHQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.182"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="LNC71_002879"
     CDS             <1..378
                     /locus_tag="LNC71_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBK6467875.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="LNC71_002880"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="LNC71_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBK6467876.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="LNC71_002881"
     CDS             1859..>2003
                     /locus_tag="LNC71_002881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467877.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAFCHQ010000176         2000 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000176 DAFCHQ010000000
VERSION     DAFCHQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.183"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            198..419
                     /locus_tag="LNC71_002882"
     CDS             198..419
                     /locus_tag="LNC71_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467878.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            420..677
                     /locus_tag="LNC71_002883"
     CDS             420..677
                     /locus_tag="LNC71_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467879.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(813..1415)
                     /locus_tag="LNC71_002884"
     CDS             complement(813..1415)
                     /locus_tag="LNC71_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK6467880.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1482..1637)
                     /locus_tag="LNC71_002885"
     CDS             complement(1482..1637)
                     /locus_tag="LNC71_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBK6467881.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          669 a          444 c          305 g          582 t
ORIGIN      
        1 tgcgccgata actaaacgaa atagacgcta aatcgcttaa aacgcaaaat agaccctttt
       61 tagagcattg attttttcta ctagtcattt cgataatata aaattgacca caacacagaa
      121 aacttatatt ataagcttat gtcgtaagtc cgacgtactc acgtaatata gacggacgtt
      181 aaatggaagg agcataaatg agagtgataa aatcagaaaa attcactttg gccgaaatta
      241 tgacgaccag cgaacttgca caagaacttg attacaacca agcttacgtt ttgcgcttag
      301 cgaaagaaca tctgactgaa ggaaaagaat atcgctctgc cggtagaaga aactatcttt
      361 tcagcccaga agcccttaaa aaactagaaa aagtcataac caccaaagaa ggtgagtaaa
      421 tgcctaaaga aacaactatc aacgttgaac aactagaaaa attgctagaa tcatattcca
      481 atgattttat cgaattgacc ggcggaaaag tcgaagaaac caccgacctc tttgacctac
      541 ttctcacaaa tattctggaa ttaaactctg aagaacaaaa agagcataag ggaaaactga
      601 tagcaccttc ctacaggaag gtggctaagt cggctgtgcc aactggtttt cttacaaaac
      661 aaccttatat tttttgatat cacaaaaaaa tccattttgg acctattttc ggtcataata
      721 tagtacctac ttttggtcat agtttcgtcc gtagttttgg tcatagtgac taagactttc
      781 acgcacaaaa aaagagctga aattaacagc tcttatcctt tatttatttt tgaatttaca
      841 cttccaccat gacgattcga ttttatatat tgcccgataa tataagctga attatcacaa
      901 agaagcggaa accaatcatc aaatctttct gacattgcat agtcaattaa atctttgatt
      961 tcagtgatat tcttagaatc aacaaacgac atcatctctc taatcaactc atatcgttct
     1021 gcactcgtta ctgacaaata actagcaatt tctgcaccac catgagcgat aatatcactt
     1081 ttagcatatt gaaatttttc tggattatcc atatgagcaa aatatcgcac catacctttt
     1141 gcattagcac atttttgcgg gtttggagac cgaagctttt gagttatctc acgtatttga
     1201 tcatacgtct ttttaccgct aaacattatc aaaacatgcc aatgaggctt tttcaattca
     1261 ccgtcaggat tcacatcttt atcatgaagt ggactctcaa cccaaggaac atgtaactca
     1321 tctaaaaact ccctccaatt ttcaggagcc gattcaggat agactacaaa cgtccaattc
     1381 cttgtgcgtt catctttagt ttttctactc gtcatgctat aattacctta attgaacaac
     1441 agtctaacag caaaagcagt gcgccaacac taatgctttt gctatttttt ttgctcattt
     1501 tcctttccgt tagcattaac ccaaattgta ataagaccag acttactaag acccatttgt
     1561 ttcgcaattt tatcaagatt atctgccact tccttagtaa gcgttacaac gactctcttt
     1621 ttttcattac tgatcataat tcgtatcctc caacgacttt ttcttaaaaa ccatagtaac
     1681 aaaaaagtaa ctaaggtgca acagtttaga tactaattag cgaataatag gccttaaacc
     1741 cttggcatta ctgattttgt caaaaaaaaa cgctgaaacc cttgggagag taaggctaaa
     1801 cagctatcga ccaatgacca atgaccaata aggggggtgt cgtagtcccc ccctcctccg
     1861 cccaattcat aaacaccaaa accaaaaaac aaacctcagg gctccgcccc gaaccccgac
     1921 ctccttctac cgaaatatat gcaggcgcca cgcttactat ttcgttcagc tatcggcttt
     1981 tgcaatctaa cacgaccgtt
//
LOCUS       DAFCHQ010000177         1979 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000177 DAFCHQ010000000
VERSION     DAFCHQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.184"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(325..528)
                     /locus_tag="LNC71_002886"
     CDS             complement(325..528)
                     /locus_tag="LNC71_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467882.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="LNC71_002887"
     CDS             complement(728..1744)
                     /locus_tag="LNC71_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBK6467883.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAFCHQ010000178         1970 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000178 DAFCHQ010000000
VERSION     DAFCHQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.185"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="LNC71_002888"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="LNC71_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBK6467884.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="LNC71_002889"
     CDS             852..1040
                     /locus_tag="LNC71_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBK6467885.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="LNC71_002890"
     CDS             1053..1610
                     /locus_tag="LNC71_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBK6467886.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="LNC71_002891"
     CDS             1642..1884
                     /locus_tag="LNC71_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBK6467887.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          718 a          305 c          404 g          543 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt
//
LOCUS       DAFCHQ010000179         1886 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000179 DAFCHQ010000000
VERSION     DAFCHQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.186"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..391
                     /locus_tag="LNC71_002892"
     CDS             98..391
                     /locus_tag="LNC71_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467888.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            388..612
                     /locus_tag="LNC71_002893"
     CDS             388..612
                     /locus_tag="LNC71_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK6467889.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            609..1634
                     /locus_tag="LNC71_002894"
     CDS             609..1634
                     /locus_tag="LNC71_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBK6467890.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT          636 a          318 c          390 g          542 t
ORIGIN      
        1 ttgtgcatta ctgaagaaga agcagataaa atcattagca aagaagagag cgcatcttaa
       61 ttgatgtgct ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg
      121 agcgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat
      181 tactgcatct ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt
      241 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
      301 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
      361 taaaacttta tttagggaga gaagaaaatg aataataaaa cgttcgaagt actaaaatgg
      421 ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg taaagcgctc
      481 aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc gtttttaggg
      541 agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag cgaagaagga
      601 ggaaacaaat gaaaaagaaa attactatta ctgcgatgag cctgttaacg gctctttttt
      661 tattacccat taatacgttt gcttatacta ttaatgacga gtataattta gcgccgaatc
      721 aaggagactc cagattagca attcctaaca aaattatttt gcacgaaact ggaatagatg
      781 caccagcaag aaacgtagcc gccaacatga aaaataatta taacggaagc aattcttata
      841 ctacagatgt tattggtgac ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt
      901 cgtggggagc tggtaacgct aatccttatg cgcctgtaca gattgaatta cagcgcacat
      961 atgataaagc attgtttgaa aaaaactatc gagcttatat tgaatataca agagatagtg
     1021 caaaaaaata tggaattcca ttgactcttg atcaaggaac ttctttattt acaaaaggaa
     1081 tcatttctca tttgtgggtg acaaattatg tttgggggaa ccacacagat ccatatggtt
     1141 acttatcgca aatgggagtt agcaaagaaa agcttgctta tgatttagct catggattta
     1201 ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat tgatccaaca cgagctggtg
     1261 cagctaatcc tacgctgaca gatggaacaa atcacgccca cattgatcag tttggggaaa
     1321 tcgaaaacgc aaacttgcat gtggctggat ggcacattgc taactataaa tacgagtata
     1381 ttttcattat ggactacaat actgggaaag aattagctcg agtaagagct gatggaattt
     1441 atagaccaga tgtaaaccaa gcttataata cttctggaaa tgttggttat catgtatctt
     1501 tcaatatgcg taattttcct agtaagaaag tctatgtaat gatgcgggca acgaatgatc
     1561 cagagggaaa cactaaaggc ggtgcgcaag atttccatga caaacgttgg tatttaaata
     1621 ttcctaaacg ataaaaatag ctcctcgttg aggagcagta cataactata ttgacaacta
     1681 taaaaattat tcgataaaat agtgatgtta tcgcatatct tcactatcac ccataaatag
     1741 tcacactcca agctatgcga taacaggttt gttgccacac attctactgg ttgattgttt
     1801 atggctttat gtggcaacaa ccagtaccct tagctcagtt ggtcagagca gacggctcat
     1861 aaccgtccgg tcgtaggttc gagtcc
//
LOCUS       DAFCHQ010000180         1841 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000180 DAFCHQ010000000
VERSION     DAFCHQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.187"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(370..1539)
                     /locus_tag="LNC71_002895"
     CDS             complement(370..1539)
                     /locus_tag="LNC71_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBK6467891.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          622 a          286 c          253 g          680 t
ORIGIN      
        1 gggatttagt ttatgatttt atatcatttc agtaactcag aatattccga cttttttgta
       61 caaaaaaatt aaccttactt atatgaagat tctaagctct tatttaaatt ttagacgggc
      121 agttatccgt agaagttctt agaatagcta atttacataa ttaaattata tgaaatttaa
      181 gtttgaacaa tatactaaga actcccctta ctataaagtg aaataaatta gcatatgaat
      241 aaccgtgcta ccaagctttt cattaataaa atatttttat taatatttta taactttacg
      301 caagatcata attttgtcgg gagcgtcaat aattttgtgt aaatggaaaa gcccattctg
      361 ttgatcaagt taaaacattg attctaatgt atcatgtatt tctttaaaac ctttatgaac
      421 tcggcataga aacttttgat tatagttgtt gaattgagat actagaaatc tctccagaga
      481 ttcttcatta ggaaattgct cttttctttt ggtatatttc tttaattgtt tgttaaaacc
      541 ttcaatcaaa ttagttgaat aaatagttct tctgattgac ggagggaaat tataaaaggt
      601 taatatagca ggattcatga gcaatttaac cactcgtgga tactgctttt tccatttatc
      661 tatcataaac cttatttgat tatttgcttc ttcttttgaa cttgcttgat aaactaattt
      721 gaaatcgcta cagatttctt gtcgatcact aacacgaacc ttatgagcga tatttctaga
      781 tatatggaca caacattgtt gaaattgagc atttggataa acactatgaa tactatcgtt
      841 aatgccactt aaaccatcag ttacgactaa taaaacctct tttaaaccac ggtcttttag
      901 gtcttgaaga atctctttcc aaacataagc agattcagtt ggagcaagac taaacccaag
      961 aacctctttg gttccatcta atcgaatgcc aattacaata taaactgctt cttttgaaac
     1021 agtttgtctt tttaaaggaa tgtgagtagc atccataaaa atgacagagt atctggattc
     1081 taaggacctt tctttaaagg caataatatc ttcagaaact attttagtca tatttgaaat
     1141 ggtttgtggc gtatagtgat gcccgtacat tttttcaatc aattcagaga tttcagacat
     1201 agtaattcct ttttgaaata gctgaataat agtggtttct aaagagtcat tggttctttt
     1261 ataagcaggt aaagtttgtt gagaaaactt accatttcta tctctaggaa ttaccaaatt
     1321 taattctcca tactctgttc tgaatgaacg tgaataattc ccattacggg aattacctga
     1381 attgaaccca gcacgatcat atttttcata atcaagaaaa gctgttaatt cagcctgcaa
     1441 taatgaatta acagctaatt ctaaatgaca tcgaaataat tcatctaaat cacctttatt
     1501 gattagtgtt tccataattt ctgtagtaaa attgggcatg agaaagtcct cctataaaat
     1561 ttcggttgtg gtaactttaa ttttacagaa tggactttct ttttttctac cctaaatatc
     1621 tatttacaca aaatatttta cgctatcatt ttgtctgtgt agatatttcg ttatatcact
     1681 aaagataata agatttaatg acctcattaa tattgtttgc taggtccaaa ctcttattaa
     1741 actttctttg ctataaaacc caaacatagt aataaaagaa taacttatta cctttaaatt
     1801 atactaccaa caaatggtag tatattatta atttatataa t
//
LOCUS       DAFCHQ010000181         1835 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000181 DAFCHQ010000000
VERSION     DAFCHQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.188"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            275..1753
                     /locus_tag="LNC71_002896"
     CDS             275..1753
                     /locus_tag="LNC71_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467892.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          669 a          255 c          326 g          585 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aacta
//
LOCUS       DAFCHQ010000182         1769 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000182 DAFCHQ010000000
VERSION     DAFCHQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.189"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(143..1162)
                     /locus_tag="LNC71_002897"
     CDS             complement(143..1162)
                     /locus_tag="LNC71_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBK6467893.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1159..1383)
                     /locus_tag="LNC71_002898"
     CDS             complement(1159..1383)
                     /locus_tag="LNC71_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBK6467894.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1380..1673)
                     /locus_tag="LNC71_002899"
     CDS             complement(1380..1673)
                     /locus_tag="LNC71_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467895.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          496 a          362 c          301 g          610 t
ORIGIN      
        1 tggcaacaaa cctgttatcg catagcttgg agtgtgacta ttatgggtga tagtgaagat
       61 atgcgataac atcactattt tatcgaatga tttttatagt tgtcaatata gttatgtaat
      121 gctcctcaac gaggagctat ttttatcgtt taggaatatt taaataccaa cgtttgtcat
      181 ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc attcgttgcc cgcatcatga
      241 catagacttt cttattaggg aagttacgca tgttaaaaga tacatgatag ccaacatttc
      301 cagaagtatt ataagcttga tttacatctg atctataaat tccatcagct cttactcgag
      361 ctaattcttt cccagtattg taatccataa tgaagatata ctcgtattta tagttagcaa
      421 tgtgccatcc agctacatgc aaatttgcat tttcgatttc tccaaactga tcaatgtggg
      481 cgtaattcgt tccatctgtc agtgtaggat tagctgcacc agctcgtgtt ggatcaatga
      541 caggcttgtt ttcagaagtt gttggatttt catcggtaaa tccatgagct aaatcatatg
      601 ctaatttttc tttacttacg cccatttcag aaagataacc gtaaggatct gtatgatcgc
      661 cccagatgtt ttgtgttacc cataaatgcg atttgattcc cggttggtta taaggagtgt
      721 ccaacgttaa tggaatacca tatttcattg ctgaatctct agccaattca acgtatgcct
      781 tgtagttttt ctcaaacgtt gctttatcat gtgtgtgttg taactcaatc tgcacaggac
      841 tgttggcatt agcatacgaa ccagcaccgt actgtacata accaggttga ccgacttgat
      901 aaacaattcc gccgtctccc acaatataag cagtataagc gctagtccat gaacgttgca
      961 tatactgcgc ttcattgcgt cctgttgctg tttcattagc cgtttcatgc agtaaaatat
     1021 actgattatt tgctacttgt gagctacctt catttgcgcc caaattaaat tcattgttga
     1081 tagtataggc aaatccgtta attggcaata aaaaaagagc cgttaacagg ctcatcgcag
     1141 taatagtaat tttctttttc atttgtttcc tccttcttcg ctttcagccg agaacatttt
     1201 gtaggttcga tttgatacac ccaacacact ccctaaaaac gcgccaaaac cagtaatgat
     1261 gacaacacag atatctgtgt actgccaatt gagcgcttta ccaactaacc ccacgaaagt
     1321 agctagtgcg ggaataatta ccagtgcgaa ccattttagt acttcgaacg ttttattatt
     1381 cattttcttc tctccctaaa caaagttttg atttgttgcg tgtgttccac caatttttct
     1441 gtatgtgtat ctaatctttc atcgtgtttc tttagttctt catgaatcat caatcgatct
     1501 gatttgctcg attctaaatc tttagtcagc aaatctaaat tgtggcttac ttttgaaaga
     1561 gtctcagtaa ttttcgagaa agatgcagca atcggtctaa ttactaataa aatcaaagaa
     1621 acgatagcgg ttattgatcc tgctatcatt ccccattccc ctaaattaat catgtgacaa
     1681 ctcctttacc ttaaataaaa acgcatcaat taagatgcgc tcttatcttt attaatgatt
     1741 ttatctgctt cttcgtctgt aatgcataa
//
LOCUS       DAFCHQ010000183         1655 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000183 DAFCHQ010000000
VERSION     DAFCHQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.190"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="LNC71_002900"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="LNC71_002900"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBK6467896.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          579 a          217 c          267 g          592 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg tatttgtcct
     1501 ttttaggtaa cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatcttttta
     1621 cgaaaaacat cacaaggaaa aacttaacta tggta
//
LOCUS       DAFCHQ010000184         1606 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000184 DAFCHQ010000000
VERSION     DAFCHQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.191"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            288..695
                     /locus_tag="LNC71_002901"
     CDS             288..695
                     /locus_tag="LNC71_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467897.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="LNC71_002902"
     CDS             709..1110
                     /locus_tag="LNC71_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467898.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="LNC71_002903"
     CDS             1112..1483
                     /locus_tag="LNC71_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467899.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          599 a          205 c          280 g          522 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcaca tcagtc
//
LOCUS       DAFCHQ010000185         1587 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000185 DAFCHQ010000000
VERSION     DAFCHQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.192"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..446
                     /locus_tag="LNC71_002904"
     CDS             24..446
                     /locus_tag="LNC71_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467900.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            456..659
                     /locus_tag="LNC71_002905"
     CDS             456..659
                     /locus_tag="LNC71_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467901.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            870..1454
                     /locus_tag="LNC71_002906"
     CDS             870..1454
                     /locus_tag="LNC71_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6467902.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          560 a          225 c          294 g          508 t
ORIGIN      
        1 ttttgatttc gaggtgatag acaatgaaaa ataagtttat tttatcttta ggtattttaa
       61 ctattggaat tagtgtaact ggaactacaa ttcctacatt tgctgatagt acaaatgaac
      121 caactactgt agtagataat aaccaagaat taactccttt agataggaac ggaatagcca
      181 ttggtaaccc aactaacgac ttaactgtta ctgaaaatta tttaatagtt tctccattac
      241 ttgctcgagc agcttctaaa acgatacgag tgacaggaac gatgattttt gataaagttg
      301 gttggccttc tagacctcct caatcacgga attggaaaga aaaaagaaat ggttattggt
      361 actatggaaa attgtattta caaagttata aatcagctgg aaacaattgg attgcgactt
      421 attcaggaac cttaactaga aagtagggat tttttatgcc agaatttgtg aacacaccaa
      481 atttttatca agatagcgat attctaagta aaattaattt accgaaagaa gaatcagctt
      541 tacaagttca gcgaacttta caggagacac aaaaaattca aaaatcaatt tctaatctag
      601 aagattctga agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa gaaaaataat
      661 gattttaaaa aaagagtgtc tagtcgacat tctttttttc taattgaaag tgttcataaa
      721 atcacccatt tctggacact ttcttttttt gctttatttt tcaaaaaaac aattcatttc
      781 atgttcataa acccataaca atatctatgt tcagttaaac tatatggata aagttatgtt
      841 acaataaaat taatgaagga gtgatttttt tgaaaatagg atacgcccgg gtatctactg
      901 gtttacaaaa tttggattta caagaagaca gcctaaataa atttggttgt gaaaaaattt
      961 ttactgatca tatgagtggg gcaaaaagta atcgaccagg attagaaatg gccattgatt
     1021 ttgttcgctc tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt agaaatatgg
     1081 aagatttgat ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt catagtattc
     1141 aagaaaatat tacgatggac aaatcaagtt ctacaggaca gctcatgttt catttgtttg
     1201 cggcatttgc agaatttgag cgtaatttga tcctggaacg atccgcagca ggacgagcag
     1261 ctgctcgtgc aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa caagatttgg
     1321 aattactgaa gactttagtg gatagcggta cacctattaa gactattgcg gaacggtgga
     1381 acgtctcaag aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa aatggaaaga
     1441 caaattctca ttagtttttg tcatttaatg ctgaaaattt ttgccattta tctagaaaag
     1501 tgacaatagt atgagaattt tttgtaagtg tctactgata acttattctc aaatgttgta
     1561 aggattttat tcatttgtta atattaa
//
LOCUS       DAFCHQ010000186         1578 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000186 DAFCHQ010000000
VERSION     DAFCHQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.193"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..1534
                     /locus_tag="LNC71_002907"
     CDS             68..1534
                     /locus_tag="LNC71_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBK6467903.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          438 a          291 c          331 g          518 t
ORIGIN      
        1 tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa gaattggggt
       61 atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg gattgtcaac
      121 tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg cattacttat
      181 ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag caacagcatt
      241 agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg gcggatgggt
      301 atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt tcattatgct
      361 tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt cgatggcatt
      421 gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg gacatcttta
      481 ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg gtgtcaactt
      541 aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata actatcattt
      601 agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca tcatccaagg
      661 acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa ctgaagaacg
      721 taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag tattcggcgg
      781 ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca gtgtattggg
      841 gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg taactgctga
      901 agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca ttttctggtc
      961 attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta cacaatctac
     1021 gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa ttttcatctt
     1081 tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc aaccatctac
     1141 agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc tattgatgtt
     1201 gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt tgggaagttt
     1261 cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg tgacaacaaa
     1321 attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag ctgatgcttc
     1381 ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag aatctgctta
     1441 cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta ctgtgaaaga
     1501 accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa caggaattgc
     1561 ttatttgcag cagttcct
//
LOCUS       DAFCHQ010000187         1568 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000187 DAFCHQ010000000
VERSION     DAFCHQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.194"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1568)
                     /locus_tag="LNC71_002908"
     rRNA            complement(<1..>1568)
                     /locus_tag="LNC71_002908"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          332 a          482 c          340 g          414 t
ORIGIN      
        1 atctctcagg aatcttactt tcttaaagaa atgggaaatc tcatcttgag gtgggcttca
       61 cacttagatg ctttcagcgt ttatcccttc cctacatagc tacccagcaa tgcccttggc
      121 agaacaactg gtacaccagc ggtaagtcca tcccggtcct ctcgtactaa ggacagctcc
      181 tctcaaattt ccaacgcccg cgacggatag ggaccgaact gtctcacgac gttctgaacc
      241 cagctcgcgt gccgctttaa tgggcgaaca gcccaaccct tgggaccgac tacagcccca
      301 ggatgcgacg agccgacatc gaggtgccaa acctccccgt cgatgtggac tcttggggga
      361 gataagcctg ttatccccag ggtagctttt atccgttgag cgatggccct tccatgcgga
      421 accaccggat cactaagccc gactttcgtc cctgctcgac ttgttggtct cgcagtcaag
      481 ctcccttctg cctttacact cttcgaatga tttccaacca ttctgaggga acctttgggc
      541 gcctccgtta ccttttagga ggcgaccgcc ccagtcaaac tgcccatctg acactgtctc
      601 ccaccacgat tagtggtgcg ggttagagtg gccataacgc aggggtagta tcccaccagc
      661 gcctccatcg aaactagcgt tccgatttct acggctccta cctatcctgt acatgcggta
      721 cagacactca atatcaaact acagtaaagc tccatggggt ctttccgtcc tgtcgcgggt
      781 aacctgcatc ttcacaggta ctaaaatttc accgagtctc tcgttgagac agtgcccaaa
      841 tcgttacgcc tttcgtgcgg gtcggaactt acccgacaag gaatttcgct accttaggac
      901 cgttatagtt acggccgccg tttactgggg cttcaattcg taccttcgct tgcgctaagc
      961 actcctctta accttccagc accgggcagg cgtcagcccc tatacttcat cttacgattt
     1021 tgcagagacc tgtgtttttg ataaacagtc gcttgggcct attcactgcg gctgatcgta
     1081 tgatcagcac cccttctccc gaagttacgg ggtcattttg ccgagttcct taacgagagt
     1141 tctctcgctc accttaggat tctctcctcg actacctgtg tcggtttgcg gtacgggccg
     1201 ctgttttctc actagaagct tttctcggca gtgtgacatc aggaacttcg gtactattat
     1261 ttccctcccc atcacagctt gtccttaaga aaagaagcat ttgactcctc ttcagactta
     1321 ctgcttggac agacatttcc gatcgtctgc attccttagc ctcctgcgtc cctccattgc
     1381 tcaaacaaaa acaacgggta caggaatatc aacctgttat ccatcgccta cgcctgtcgg
     1441 cctcggctta ggtcccgact aaccctgggc ggacgagcct tccccaggaa accttagtca
     1501 ttcggtggac aggattctca cctgtctttc gctactcata ccggcattct cacttctaag
     1561 cgctccag
//
LOCUS       DAFCHQ010000188         1558 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000188 DAFCHQ010000000
VERSION     DAFCHQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.195"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..344
                     /locus_tag="LNC71_002909"
     CDS             132..344
                     /locus_tag="LNC71_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467904.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="LNC71_002910"
     CDS             506..1024
                     /locus_tag="LNC71_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBK6467905.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="LNC71_002911"
     CDS             1031..1543
                     /locus_tag="LNC71_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467906.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          613 a          181 c          242 g          522 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaa
//
LOCUS       DAFCHQ010000189         1418 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000189 DAFCHQ010000000
VERSION     DAFCHQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.196"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..962
                     /locus_tag="LNC71_002912"
     CDS             <1..962
                     /locus_tag="LNC71_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBK6467907.1"
                     /translation="GTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLIS
                     KITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLP
                     VKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVI
                     LQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVN
                     ELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLI
                     FILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            964..>1418
                     /locus_tag="LNC71_002913"
     CDS             964..>1418
                     /locus_tag="LNC71_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467908.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSI"
BASE COUNT          480 a          167 c          236 g          535 t
ORIGIN      
        1 caggaacgcc aggattactt gcaagacaag gttttattga acatcggtta tttggtgtct
       61 atacaagtcc aaatgttgga gctatgtttg gatatacttc tattatttta agtttaatta
      121 gtgtattaat ttcaaaaata acaaaaacga ttcgtgtttt ttatatgatg aattttatcg
      181 tacaaatatt ttatattaca ctttcttcgt ctagaggtgc ccaggtagta atttgttctt
      241 ttttagtatt atttcttttg ttgtttgcca attcaaaatt tagaaagagc attagagaag
      301 tactacctgt aaaaatcaaa tggatggtaa gcatattatt agttctattg gtatattttt
      361 cttttggtac taattatgct aaggattttt tagcaacaat tcctgtatcg attgatagaa
      421 aagtagaagt tatagagtca aatgaatcag ataaaactcc acttccagaa aaagaagtaa
      481 tattacagca ttctagtgat aactccgaaa tttcagctgg tcgttttacg atttggaaag
      541 ctgcgcttaa agttataaga ccaactttgt tatttggtta tggagagacc gatttctatg
      601 ggaatagcga tgcaaaattt ttgaaaaaag tagatcttga tactacagat gtgaatgaac
      661 taaaaagagc acatggaaac atgcataatg gatttcttca agtacttgta tcaagtggta
      721 taattgcttt tatatgtatc tatgctttct atgtgttaaa tatctttagt ttactcaaaa
      781 cattttttag aagtaaagtg aattatgcgt ttcttggtat tgttttaatt tttatactat
      841 ctatttttat agatgaaatg gtggaggctc atgtactttt caataagcgc gatgtcattt
      901 caattgtttt ctggtattat ttgggaagca tttcttactt gtatttaaaa aaggagaatt
      961 aatatgaaga cagctttcat tgcttggact cctttacatt tgattaatat attaaatgtt
     1021 tatcaaaatt tttaccgaga atcaaaagga gatttattta tttattctga atttgaaaac
     1081 gctagaagat atagtgagcg tataaaagaa ttagggctat ttaatgatgt tgtttttgtg
     1141 aatcaccgga aaatcggttc ttcctttaaa cgtaaaatta acttgttaac taaccataat
     1201 caatttttct cggatgattt atccgtttat gatgaaattt ttatacaggg ggaaaattat
     1261 ttcacaaaaa tacttttctc caatgtttac aaaaaaaatc cagatgtaaa actaaattat
     1321 atagaagatg gtttgggtac ttatttgaag aagagtatat tttcaacaag taaaaacgtt
     1381 caaagagttt ttaaaatatt aaataaaaat agcatctt
//
LOCUS       DAFCHQ010000190         1417 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000190 DAFCHQ010000000
VERSION     DAFCHQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.197"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..1344
                     /locus_tag="LNC71_002914"
     CDS             52..1344
                     /locus_tag="LNC71_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6467909.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHSKTFLT"
BASE COUNT          444 a          285 c          271 g          417 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aactacgaat tatgattcgt
     1321 catagcaaaa catttttaac gtaagcgccc catagaacag taccttacta acaaatgcac
     1381 ctcaagatat acttgagcta tcaagaagtc tggaggt
//
LOCUS       DAFCHQ010000191         1417 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000191 DAFCHQ010000000
VERSION     DAFCHQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.198"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="LNC71_002915"
     CDS             complement(150..1337)
                     /locus_tag="LNC71_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBK6467910.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAFCHQ010000192         1368 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000192 DAFCHQ010000000
VERSION     DAFCHQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.199"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..1334
                     /locus_tag="LNC71_002916"
     CDS             33..1334
                     /locus_tag="LNC71_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBK6467911.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          445 a          275 c          252 g          396 t
ORIGIN      
        1 atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatcat atcaaactac
       61 tacttggaat caaagataaa aacattttca tcacaaatgt ggaatcgaag agcattaaga
      121 atatcaagag tcttgttgtt tctgcgacgt taagtaaaga aattcgtcgc tgtcctttgt
      181 gtaaacagat gaatcatgaa ggcatgatcg ttaagaacgg aaagaaaaaa tcgctcattc
      241 aacttaataa atgtgcgaac caactgactt atcttgcttt agcgaaacaa cgctaccact
      301 gtcgtggctg ccacacctat tttacggcga atacctacat tgtcgataga aactgcttta
      361 tcgctaaaca agtgcgctat aaaattttag aagagctaac tgaaaaacaa gcaatgacga
      421 caattgctaa gcattgcggc gtttcttggt cgaccgtttc tcggacactc gcttctctga
      481 tccccatgac aaaagtaaaa agaaattggt taccgcgttg tttattaatg gatgaatttc
      541 gttcactgaa aaatcaagtc ggtccctact ccttttcttg tatggatggc gatactggaa
      601 aattactgga tattcttcct tcacggaaga aaaaggctct cgtttcttat tttatgcaat
      661 ttgaacgacg cgcacgattg aaggtaaagg tcatcgtgac ggacatgaat gtttcttatg
      721 cctctctgat aaaggaatgt tttccaaagg caaaactcgt tgtggatcgt tttcacattg
      781 tcaaacatct cattcgtaaa tttgaggata ttcgcgtacg aatcatgaaa agctttgatc
      841 gaaacgatcc aacacaagcc aaatattatc gacaattaaa ggcgttgtct cggcttctga
      901 tcaaacgaca ggacaagctt gtttatgaca aatggaccaa atggcgcaat tttggttggg
      961 cgtatctcac cgaaagtgaa gtggtggatc gtctgctttc gatctctgac gaattacgaa
     1021 tcgcctattt ttactatcaa gaaattcttc aagcgtttca cgacaaagag gcggacactt
     1081 tctttaaact tgtaagaacg atgcccaatt ccgttccaaa agaactgcat tctataaaaa
     1141 aagcttttat aaactatgaa tccggcattc gtttagccct cgaattgcct tattcaaatg
     1201 ccaaaataga aaatctgcat acccacatca aagcattgaa acgagtcgct tatggctttc
     1261 gaagctttcg gaaaatgaag accagaattt tcttactaaa taatctgatt acctatgaat
     1321 caaaaaacat ctaaagtaga aaaggaattc taccttagat gttcttat
//
LOCUS       DAFCHQ010000193         1364 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000193 DAFCHQ010000000
VERSION     DAFCHQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.200"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            80..>1364
                     /locus_tag="LNC71_002917"
     CDS             80..>1364
                     /locus_tag="LNC71_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBK6467912.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          465 a          263 c          257 g          379 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta atca
//
LOCUS       DAFCHQ010000194         1362 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000194 DAFCHQ010000000
VERSION     DAFCHQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.201"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..1310
                     /locus_tag="LNC71_002918"
                     /pseudo
     CDS             37..1310
                     /locus_tag="LNC71_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          447 a          279 c          240 g          396 t
ORIGIN      
        1 ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa
       61 ctattactag gaatcaaaga tggaaacatt caaatcactc atgtcgagaa gaaaaaaata
      121 cattcaatca acagtatcgt tgtttctgcc acgctcatta aaacgattca gccctgtcct
      181 cagtgtaaac aggtgaatca acatgggctg atcgttaaga acggaaagaa aaaatcgctg
      241 gtccttctca ataagtgtgc gaatcaacgg acttatcttg ctttggcaaa gcaacggtac
      301 ctctgtcgtg gctgtcacac ctattttacg gcgaatacct acattgttga aaaaaactgt
      361 tttatcgcca aacaagtaca ttataaaata ttggaggaat tgactgaaaa acaagcaatg
      421 acgacgattt ctaaacattg cggcgtttct tggtcaaccg tttcgcgaac actcgcttct
      481 ctgctcccca tgacaaacgt gaaaataaat tggttgcctc gctgttcatt ggtagatgaa
      541 tttcgttcat tgaaaaatca agtcggtccc tattcttttt cttgtatgga tggcgatact
      601 gggaaattac tggatattct tccttcacgt aagaagaaag atctcatttc ttattttatg
      661 caatttgaac gacgtgcacg tttaaacgta aaaatactcg taacagatat gaacgcctct
      721 tatgctttgc tgataaaaga gtgctttcct catgccaaac tcgttgtgga tcgctttcac
      781 atcgtcaagc acctcattcg cagttttaag gatatttgac tatgagtcat gaaaagcttc
      841 gaccgaaacg atccaataca agccaaacac tatcgacaag tgaaggcttt atctcgactt
      901 ctgatcaaac gacaggacac gcttgtttat gataaatgga caaaatggcg taattttggt
      961 tgggcctatt taaccgaaag tgaagtcgta gaacgtctac tttctacttc tgatgaacta
     1021 cgaatagctt atgcttacta tcaagaaatt cttcaagctt tttatgacaa agaagcggac
     1081 atcttctttc agcttgtaaa aacaatgccc aagtcggttc caagagaact gcatcatata
     1141 aaaaaagcgt ttataaacta tgaatccggc attcgtttag ccttagagtt gccttactca
     1201 aatggcaaaa tagaaaatct gcatacccac atcaaagcat tgaaacgaat agcttacggg
     1261 ttaggatgag aaccagaatt ttcttaatca atcatctgat cacttattaa acataaaaaa
     1321 acatctaaag cagaaaatca attctacctt agatgttctt at
//
LOCUS       DAFCHQ010000195         1359 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000195 DAFCHQ010000000
VERSION     DAFCHQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.202"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..208)
                     /locus_tag="LNC71_002919"
     CDS             complement(<1..208)
                     /locus_tag="LNC71_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467913.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
     gene            complement(305..757)
                     /locus_tag="LNC71_002920"
     CDS             complement(305..757)
                     /locus_tag="LNC71_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467914.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(764..1312)
                     /locus_tag="LNC71_002921"
     CDS             complement(764..1312)
                     /locus_tag="LNC71_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBK6467915.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          487 a          264 c          132 g          476 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
      361 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
      421 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
      481 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
      541 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
      601 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
      661 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
      721 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
      781 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
      841 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
      901 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
      961 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
     1021 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
     1081 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
     1141 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
     1201 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
     1261 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
     1321 ctccataaaa aataagttac tttttaaata tcttgttta
//
LOCUS       DAFCHQ010000196         1344 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000196 DAFCHQ010000000
VERSION     DAFCHQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.203"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="LNC71_002922"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="LNC71_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBK6467916.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="LNC71_002923"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="LNC71_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          515 a          222 c          219 g          388 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atctcttttt
     1321 caaaataaac ataaaagccc ttta
//
LOCUS       DAFCHQ010000197         1344 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000197 DAFCHQ010000000
VERSION     DAFCHQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.204"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(60..278)
                     /locus_tag="LNC71_002924"
                     /pseudo
     CDS             complement(60..278)
                     /locus_tag="LNC71_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="LNC71_002925"
     CDS             complement(544..738)
                     /locus_tag="LNC71_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467917.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(838..924)
                     /locus_tag="LNC71_002926"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="LNC71_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          480 a          218 c          180 g          466 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttt
//
LOCUS       DAFCHQ010000198         1298 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000198 DAFCHQ010000000
VERSION     DAFCHQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.205"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="LNC71_002927"
     CDS             7..624
                     /locus_tag="LNC71_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467918.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          488 a          167 c          205 g          438 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttatt
//
LOCUS       DAFCHQ010000199         1294 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000199 DAFCHQ010000000
VERSION     DAFCHQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.206"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1244
                     /locus_tag="LNC71_002928"
     CDS             <1..1244
                     /locus_tag="LNC71_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBK6467919.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAFCHQ010000200         1275 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000200 DAFCHQ010000000
VERSION     DAFCHQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.207"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1203)
                     /locus_tag="LNC71_002929"
     CDS             complement(31..1203)
                     /locus_tag="LNC71_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HBK6467920.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DAFCHQ010000201         1246 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000201 DAFCHQ010000000
VERSION     DAFCHQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.208"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="LNC71_002930"
     CDS             200..742
                     /locus_tag="LNC71_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467921.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="LNC71_002931"
     CDS             898..>1246
                     /locus_tag="LNC71_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBK6467922.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAFCHQ010000202         1193 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000202 DAFCHQ010000000
VERSION     DAFCHQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.209"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="LNC71_002932"
     CDS             <1..1076
                     /locus_tag="LNC71_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBK6467923.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFCHQ010000203         1159 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000203 DAFCHQ010000000
VERSION     DAFCHQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.210"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            16..276
                     /locus_tag="LNC71_002933"
     CDS             16..276
                     /locus_tag="LNC71_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467924.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(638..877)
                     /locus_tag="LNC71_002934"
     CDS             complement(638..877)
                     /locus_tag="LNC71_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467925.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          408 a          163 c          217 g          371 t
ORIGIN      
        1 aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata tatttgccaa
       61 cctattggct tgaaacaaaa agtggttgga gaagtaatca gtaaattaga aaattgtgtt
      121 gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga
      181 aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata tctatataac
      241 tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag tgccttcaaa
      301 agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg ttcatgattt
      361 ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc tggtggagaa
      421 actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa actagtagtt
      481 ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc aggatcattt
      541 agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg agtaaacgcg
      601 aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt tgtttgaaat
      661 aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa acttttatat
      721 cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg atactattcg
      781 aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca gtaagtaaca
      841 ataaatttga taaactgata aacagaattc ttttcattat gtacacactc cttatagtat
      901 cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt
      961 tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc agttcaaacc
     1021 agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt tttatgaaaa
     1081 aatttataag tgaaaacgta ggtatgatat tatctagagg taattttttt gggtatttaa
     1141 atagttgcta ggaggatag
//
LOCUS       DAFCHQ010000204         1137 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000204 DAFCHQ010000000
VERSION     DAFCHQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.212"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..355
                     /locus_tag="LNC71_002935"
     CDS             <1..355
                     /locus_tag="LNC71_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467926.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="LNC71_002936"
                     /pseudo
     CDS             615..910
                     /locus_tag="LNC71_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="LNC71_002937"
     CDS             985..>1137
                     /locus_tag="LNC71_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBK6467927.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAFCHQ010000205         1110 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000205 DAFCHQ010000000
VERSION     DAFCHQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.213"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..234)
                     /locus_tag="LNC71_002938"
     tRNA            complement(161..234)
                     /locus_tag="LNC71_002938"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ile,seq:cat)
     gene            complement(789..1103)
                     /locus_tag="LNC71_002939"
     CDS             complement(789..1103)
                     /locus_tag="LNC71_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467928.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          418 a          174 c          170 g          348 t
ORIGIN      
        1 tcaaatatac agaagtataa ctatgtgaaa catcctttca ttaatccata aaaggataca
       61 taaaaaagcc actcatttga gtggcaatga agaaaagctt tagcttgtat aatactcttc
      121 aaaaaattct aacacagaac gattcaattg gctacattaa tgtaccctgt aggactcgaa
      181 cctacgaccg gacggttatg agccgtctgc tctgaccaac tgagctaagg gtagtggttg
      241 ttgccacata aagccataaa caatcaacca gtagaatgcg tggcaacaaa cctgttatcg
      301 catatcttgg agtgtgacta tttatgagtg atagtgaaga tatgcgtctc catatatgta
      361 tggttgagac agataaacat gtatgaaaaa cgttgttaaa tcaacgtttt tttattttgg
      421 tcaaggagag aaaagtagag aaatacaaaa ttttttgcat tgattttgca ttcaaaaaaa
      481 cattcaaaca tttagaaatc cccaatacgt attcaggaaa atctatttta aggtttacaa
      541 aaagaacttt tgttcgtaca ctctttttga ggtgaacttt tatgctgatg gaaggaaaaa
      601 cacaattatg gtttaagcat gttccttcaa ataagttttt taattataaa ctctagagaa
      661 attaattcat cctaacataa aataattgtt cactcaaaag tttttctaga acaattaaaa
      721 cagcaggctt cacattcgct atacatttgt aagaagtaat aattcctgta ctaaaaggca
      781 taattgcttt aattattttt tgacttctct tcaaacacaa gaagtagttg tgtaataatt
      841 gcagaaataa ggattccaat aatagatata aaatttgaca tggaaacgtc tataacatta
      901 aaagaataaa gtgttgaaga aagtaaactg attgaaataa cccctccgta aaaaatagtc
      961 tgtgaatata cccacttttt ttcagaagac gtacttcgtt tggttctaaa acctatccct
     1021 gcatttctac tcttatcaaa aaaccaaata tttagaagta ataatattgc tattattaag
     1081 ataaaattac tgtaaaaatc caaaagctat
//
LOCUS       DAFCHQ010000206         1110 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000206 DAFCHQ010000000
VERSION     DAFCHQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.214"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(265..969)
                     /locus_tag="LNC71_002940"
     CDS             complement(265..969)
                     /locus_tag="LNC71_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467929.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          321 a          193 c          199 g          397 t
ORIGIN      
        1 tttttctcta tcagaaaata tgtttattat tagaattttt tatagttaag gttaggcatc
       61 ttaagaaatc aggcaactcc aagatatctt caggtagaag agaagtcgcc taacattgta
      121 ggatcatagg tgatttgttg gacaggtcaa gctgatattt gagcctgatg ttagaaggaa
      181 tttcttttta cttgctgtag aaattattat atgactaaaa aggctgggat tttaaatccc
      241 agccttttat gacaaataga atttttatcg atcagtaatc gctgtcgctg caaattgttc
      301 gatcaaagga atcaaagtca atttgacttt gtatttccgt tcgagtgctt tttcgataac
      361 gaactcattt tcagtagtca tagaaatagt tgctaattta cgaggttcat cagcattttt
      421 ttcattaata actacttcat gttcattttg ttctgtgtaa aatttaggat actcagcaac
      481 ggcttttgaa aaatcagcaa aacttaaagc ggcattcgta tcagttgagc gtgtattttc
      541 agttgtgtcc gcttttggtt tttccgtttt ttcttctggc acactgactg tttcttctgt
      601 aactggtgtc gcaacagctt gcgcagttgg ttcatcgttt ttcaatcgtt gatcattcca
      661 gtaagacacg atttcaataa gaagattgtc acggaatcgt tcgaaaatcc cgttttctcc
      721 tgaacgaatt ctctttaata cagtattgaa gttgtcatcg attggcacgt tgatccgtgt
      781 atcttcattt gttttcccag ctaatccgat cgttaagatt tgacggtttt tgggatcaag
      841 catgacttgt aacggcaaac cataacgtcg caagctctct tcttctagag aacggaataa
      901 ttgtcctctt acaccatcca atgaaggacg gaattgattt aataattttt gtttctgttt
      961 tttcttcata tgttctctcc ttcgctcata ccctttcgat tataagaaaa aaacagccaa
     1021 atgaaaagga gtaatgcaaa aaaacttgcg tatacgcaaa ttacaacaga aaaatttgcg
     1081 tattttcccg ttttttgaaa gaaaaataaa
//
LOCUS       DAFCHQ010000207         1073 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000207 DAFCHQ010000000
VERSION     DAFCHQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.215"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1073
                     /locus_tag="LNC71_002941"
     rRNA            <1..>1073
                     /locus_tag="LNC71_002941"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAFCHQ010000208         1062 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000208 DAFCHQ010000000
VERSION     DAFCHQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.216"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..993)
                     /locus_tag="LNC71_002942"
     CDS             complement(37..993)
                     /locus_tag="LNC71_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467930.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAFCHQ010000209         1002 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000209 DAFCHQ010000000
VERSION     DAFCHQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.217"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            369..1002
                     /locus_tag="LNC71_002943"
                     /pseudo
     CDS             369..1002
                     /locus_tag="LNC71_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAFCHQ010000210          991 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000210 DAFCHQ010000000
VERSION     DAFCHQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.219"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..94)
                     /locus_tag="LNC71_002944"
     tRNA            complement(21..94)
                     /locus_tag="LNC71_002944"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="LNC71_002945"
     tRNA            complement(108..181)
                     /locus_tag="LNC71_002945"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="LNC71_002946"
     tRNA            complement(209..281)
                     /locus_tag="LNC71_002946"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="LNC71_002947"
     tRNA            complement(301..374)
                     /locus_tag="LNC71_002947"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="LNC71_002948"
     tRNA            complement(384..457)
                     /locus_tag="LNC71_002948"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="LNC71_002949"
     tRNA            complement(468..553)
                     /locus_tag="LNC71_002949"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="LNC71_002950"
     tRNA            complement(570..641)
                     /locus_tag="LNC71_002950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="LNC71_002951"
     tRNA            complement(653..725)
                     /locus_tag="LNC71_002951"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="LNC71_002952"
     tRNA            complement(743..824)
                     /locus_tag="LNC71_002952"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="LNC71_002953"
     tRNA            complement(832..904)
                     /locus_tag="LNC71_002953"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="LNC71_002954"
     tRNA            complement(908..980)
                     /locus_tag="LNC71_002954"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DAFCHQ010000211          987 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000211 DAFCHQ010000000
VERSION     DAFCHQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.220"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            25..>987
                     /locus_tag="LNC71_002955"
     CDS             25..>987
                     /locus_tag="LNC71_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBK6467931.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPP"
BASE COUNT          356 a          185 c          169 g          277 t
ORIGIN      
        1 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
       61 gtcactaaag gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca
      121 ctcctacgga ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt
      181 aattcaggta attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa
      241 ttaacattgg aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc
      301 tacaaaagaa caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt
      361 acgatgtctg aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa
      421 accatgtcca acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc
      481 ttaaatgata agtatgtcgc tatttttatg gacgctactt acattccact aaaacgtcaa
      541 accgtatcca aagaagcgat ttatattgcc attggtatac gagaagacgg cactaaagaa
      601 gtactgagtt atgcgattgc tccaactgaa tcaacatacg tttggaatga gctgctacag
      661 gatattaact ccagaggagt tcaagaagtc ttgcttttta ttacggacgg cttaaaaggc
      721 atgaaagata ctatccatca aatttatcct aaagcaaaat atcagcattg ttgtatccat
      781 gtatctcgta acatcgctca taaagtacgt gtcaaagacc gaaaagaaat ctgtgatgac
      841 tttaaggctg tttatcaagc taactcaaaa gaagaagcga ataccttctt atccggcatg
      901 attgagaaat ggaagaaaaa ctatcctaaa gtgacgcagt cactcataga aaaccaagac
      961 ttattaactt tttatgattt tccacct
//
LOCUS       DAFCHQ010000212          961 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000212 DAFCHQ010000000
VERSION     DAFCHQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.221"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            172..459
                     /locus_tag="LNC71_002956"
                     /pseudo
     CDS             172..459
                     /locus_tag="LNC71_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            473..904
                     /locus_tag="LNC71_002957"
     CDS             473..904
                     /locus_tag="LNC71_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467932.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DAFCHQ010000213          911 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000213 DAFCHQ010000000
VERSION     DAFCHQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.222"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..>911)
                     /locus_tag="LNC71_002958"
     CDS             complement(161..>911)
                     /locus_tag="LNC71_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBK6467933.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTV"
BASE COUNT          279 a          177 c          159 g          296 t
ORIGIN      
        1 tccttccata tagatttttg aattacacac tagtttaaac taaaacaaac aaggaaaaac
       61 ttaccattta atcataaaat tagctcatat gaatatatta gaatgtaaaa tagactggtc
      121 aaaatctggt caaaaaagag gactaataca ggtagtcctc tcagactgta gacaaaagct
      181 gttccaaact cgtaactcga gcttggaaca gctttttatc tacttcgttt cgaatttttg
      241 aacaaaaaaa taaaggctcc ttgcctttcc atagccaggt ggctaatttc tttaaattca
      301 ttgcagcaaa cactagcatc gtatgcgtcg cgactttttc caagcctcga tacttggtcc
      361 aacgcatccc atgcttttct tttgcatccg caaatattcg ttcaatcgtt tgtttccgtt
      421 ttttgtaaat tgatttattc agatctgtta ggcgaagatg ttccacttca tccatcagat
      481 ctccccaaac atgtctcgtg atgactcgtt gatgattttt acttttggta cattggttta
      541 gcagaggaca gttcacacat tcttttggat tggatttgta ttctcggtac ctttctcgag
      601 taaccgttga aaaagcgagc atttgatctt gcggacaaat ataacaatca aaatactcgt
      661 catagagaaa ctcatttttt gataatagcc cctttttccc tttgggcctt gaataaggca
      721 aacaaggtcg aaggtttaaa tgagtcaaaa agtgaacaaa cttaggtgtc ttatactcag
      781 catcagcgac aacacaattg atttcaggaa aaagtctctt cgattgtttg atgagctgaa
      841 aggcgacttg actgtcatga atatttctag gagtgatgat tgtgctaaga ataaatccat
      901 tgtcgtcgca t
//
LOCUS       DAFCHQ010000214          895 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000214 DAFCHQ010000000
VERSION     DAFCHQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.223"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            252..875
                     /locus_tag="LNC71_002959"
     CDS             252..875
                     /locus_tag="LNC71_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6467934.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          330 a          129 c          167 g          269 t
ORIGIN      
        1 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
       61 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
      121 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
      181 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
      241 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
      301 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
      361 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
      421 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
      481 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
      541 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
      601 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
      661 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
      721 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
      781 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
      841 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aagaa
//
LOCUS       DAFCHQ010000215          878 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000215 DAFCHQ010000000
VERSION     DAFCHQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.224"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            454..852
                     /gene="spxA"
                     /locus_tag="LNC71_002960"
     CDS             454..852
                     /gene="spxA"
                     /locus_tag="LNC71_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBK6467935.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          331 a          115 c          149 g          283 t
ORIGIN      
        1 atttgattta tttgtttata gtaaatttta tttagtaatc attgctttaa aatactacgc
       61 ttatataaca gagaaaataa gcgttataat aaagaacagt attatttaaa atgacagaaa
      121 acattactat taaagaaata aaaaacataa aaaatcttaa gaaatcggtt tcatttagct
      181 tttgagataa aataatagta cagttttata cgctgtttca tatgtttaga aaatttgaat
      241 tagatttgaa aactctaatt tttaaatgat aaaaaaagcg agaatgctgg ttttactgta
      301 tattaacctt agataacttt cattttttta aaaaaattat gaaaaaaagg tagctattta
      361 aaagttgatt atgtataata gaaacagatg ggagattatt atgatcttct gttacaaaaa
      421 agtgattctt atcacgaaaa ggagtggtgc acgttgttaa cattatatac ttccccaagt
      481 tgtacttcct gcagaaaggc tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa
      541 cgtaatattt tttcagaacc attgaacagt tctgaattaa aagcgatttt acaaatgaca
      601 gaagacggaa cagaagaaat catctcgaca cgttccaaag tattccaaaa actgaacatg
      661 gatttagatg atctgccgtt acaagaatta ttggaacttg tgcaaaacaa tcctggattg
      721 cttcgccgtc caatcatgat tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa
      781 attcgtcggt tcctaccgcg cgatgtacga caattagaat tacgtcaagc acaattgatg
      841 gctggattat agaaggataa tacgaaactt ggaaaaga
//
LOCUS       DAFCHQ010000216          866 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000216 DAFCHQ010000000
VERSION     DAFCHQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.225"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            35..>866
                     /locus_tag="LNC71_002961"
     CDS             35..>866
                     /locus_tag="LNC71_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6467936.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          298 a          151 c          167 g          250 t
ORIGIN      
        1 gttccgacca aagaattata aggagtgagt acaaatgacc tatacccatc ttacaacgga
       61 tgaacttgta ataatagagt cttatttcaa aatgaatcaa tctgttgcta aaactgccca
      121 ttgcttgaat cgttcaagac aaacgatcca taaagtatat ctgttcttca agcaaggaaa
      181 atcagcctta gaatattatc aacagtataa gaaaaacaaa tcaaactgtg gtagacgtcc
      241 gcttgtttta cccgaggaac aatcagaata tattcaaaga aaggttgttc aaggatggac
      301 acccgatgtg attgttggtc gtgcagcgtt tcctattagt tgttctgctc gtaccattta
      361 tcgtatgttc aaaaaggggc tatttgattc ttctgactta ccgatgaaag gcaagcgtaa
      421 accgaatgga catcaagaaa gacgtggaaa acaaactttc cgccgctcta ttcatgaacg
      481 tgaaaaggat tatagccaat tctcaaatga gtttggtcac cttgaaggtg acactatcgt
      541 aggtctgaaa cataaaagtg ctgtaattac cttagttgaa cgattatcaa aagttatcat
      601 cacattgaaa ccgtgtggta gacaagcgat tgatattgaa aaaaaattaa atcaatggtt
      661 tgaatctgta ccgaaaaacc tattcaaatc catcactttt gattgtggaa aggaattttc
      721 aaattggaaa cagatcagta atgtcaatga tattgccatt tatttcgctg atccaggaac
      781 gccgtctcaa agaggcctaa acgagaattc taacggattg ttacgtagag atggtttatt
      841 gaaatctatg gatttcaatt cagtag
//
LOCUS       DAFCHQ010000217          768 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000217 DAFCHQ010000000
VERSION     DAFCHQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.226"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..732
                     /locus_tag="LNC71_002962"
     CDS             19..732
                     /locus_tag="LNC71_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467937.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT          254 a          150 c          137 g          227 t
ORIGIN      
        1 aataatgagg tattttatat gaaaaagtta ggttttattt tattgttggt ctctttattc
       61 cttgtaggat gtacgtctcc tagtaaagaa aatagtgatc catctgcgac cacagacaaa
      121 agttcaatca gacaaaccag ttcagctgaa aaatcaagta ctcagaaatc agcttctact
      181 gaaacgaata catcttcaac aagcagtaaa gtctcagagc ccaatacttc ttctacatct
      241 agtagtacag ttgagacaaa tatttcttct tcaacaatag acacaacctc ctcacagtct
      301 tctcagtcaa tagatccgtt agcaggatat tctgatctac aaattgagta cgcacgtgtt
      361 tggctagcta ttagaggtaa tacttttaga gatacatcgg gtgatttcga attacatgtt
      421 attcattatc ctgcaggaac acctatcgcc ccttatgatg cagggagtgc agtctatccg
      481 aaagatgtgg ttatgctcac tgggaaatac agttaccagg gagttttagt gtattcaagt
      541 aaccatgacg ggacaatcac tagttatcca gttccttcac attggcaaat gcctgcagat
      601 caaacatctt atccagcgtt cattagagaa acaactcaag aaatcattga taatgcatct
      661 gtagtagcta ttcctacagg aaatcccaat gacgtgaaac agttaatcga tgtgacagtt
      721 atagataatt agactttcta atatgttata caaaaagaag ctgacagc
//
LOCUS       DAFCHQ010000218          765 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000218 DAFCHQ010000000
VERSION     DAFCHQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.227"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..288)
                     /locus_tag="LNC71_002963"
     CDS             complement(<1..288)
                     /locus_tag="LNC71_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HBK6467938.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIY"
     gene            complement(281..>765)
                     /locus_tag="LNC71_002964"
     CDS             complement(281..>765)
                     /locus_tag="LNC71_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBK6467939.1"
                     /translation="AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMY
                     EQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSK
                     LANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE
                     "
BASE COUNT          273 a          121 c           89 g          282 t
ORIGIN      
        1 ataaatagat ggaattaaaa aattttctct gttaatgatt atagttaaga taaaactaat
       61 cattgctcca atcagtagcc aagaaaaacg cattttaaaa aaatttttct ttataaaaac
      121 ctcatacaaa actataattc caccccaagc aaaagctatt ttcatcggga tattagttac
      181 attaaaagta ctaggcataa aataaagcag acaaatgatt aaatttatta ctcgaaacgc
      241 atctcgactt aataaactct tctctaaaac aggtactatt ctattcatct atatttccaa
      301 ctttcaaaat ttctttatca attctattta atagtttatc agagtaatcc gatttaactt
      361 ttcttacaaa taatttttca ctttctttta tttgatctat atccctttca tcaaaccatt
      421 tgttcatgga aggatcaata atatgaatat ttgctaattt cgaagtttgt aaagtagttt
      481 cttcaatatt tttcaattca agtggtaaat ctggtcctcc aataacattt ttatttttga
      541 acgaggaatt aaaaaaaagt gtatgaaaaa atttttcatc tggggcaaac atacctattt
      601 taaaattcag gtagtcttgt tgattttgtt catacatttg tattaattct gttaggcatt
      661 ctttcgacaa agcccaccat tgagaaccat gatacaaatc aaatttatgg tgtctaattt
      721 ttgggggaag tttaatcatc ctcagacaac gattaattat agctc
//
LOCUS       DAFCHQ010000219          746 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000219 DAFCHQ010000000
VERSION     DAFCHQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.228"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(227..498)
                     /locus_tag="LNC71_002965"
                     /pseudo
     CDS             complement(227..498)
                     /locus_tag="LNC71_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            609..>746
                     /locus_tag="LNC71_002966"
     CDS             609..>746
                     /locus_tag="LNC71_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBK6467940.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          253 a          129 c          110 g          254 t
ORIGIN      
        1 attttgaaat aagactctat tattacaagt tcatccgttg taagatgggt ataggtcatt
       61 tgtactcact ccttataatt ctttggtcgg aactattttg agtgtagcac aaatgacttt
      121 tttagttgtc tagcttaatt ttacaatcgg cgatggcaaa aatttcaatc tttaaatggc
      181 gaaaaacaaa gagcgtgaga ccctatcctt atctcacgct ctttgtctat ttttctaaca
      241 aatcaagtat ttcttgagga gaatcaataa ttatatctac actttgaaaa ttttctatat
      301 ttttggctcc ccatttagct aaaccaaatg ctatcccaca agctttcgca ctttgcatgt
      361 catataatga atcaccaata taaatcgttt cttatttttt atgcccaatt catcaattgc
      421 cttttgtatg gggtccggaa aaggtttatg caacttagta tctgaagctg taatttgtat
      481 atcaaaatat agatttaacc cgtggtgcta gttaattttt tcctagagga aaatgtctaa
      541 acatgttggt aaatcaacaa aaaaacgcat atagtagtct caaaactccc aagaaatgag
      601 gactacatat gcgttcatca aaatatactg aacattatac agatactttt ataacattca
      661 aggaaattat gagaactgtt tctaacatat accacaattg tgtaccagat aagattaaaa
      721 accgaagaaa tactgatcaa ctgaag
//
LOCUS       DAFCHQ010000220          725 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000220 DAFCHQ010000000
VERSION     DAFCHQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.229"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..>725
                     /locus_tag="LNC71_002967"
     CDS             28..>725
                     /locus_tag="LNC71_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBK6467941.1"
                     /translation="MLGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWD
                     GKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQA
                     AYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQ
                     CYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYD
                     QLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAFCHQ010000221          683 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000221 DAFCHQ010000000
VERSION     DAFCHQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.230"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>683
                     /locus_tag="LNC71_002968"
     rRNA            <1..>683
                     /locus_tag="LNC71_002968"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          166 a          153 c          214 g          150 t
ORIGIN      
        1 gtccggattt attgggcgta aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc
       61 cccggctcaa ccggggaggg tcattggaaa ctgggagact tgagtgcaga agaggagagt
      121 ggaattccat gtgtagcggt gaaatgcgta gatatatgga ggaacaccag tggcgaaggc
      181 ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga
      241 taccctggta gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc
      301 agtgctgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact
      361 caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg
      421 cgaagaacct taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc
      481 gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt
      541 aagtcccgca acgagcgcaa cccttattgt tagttgccat cattcagttg ggcactctag
      601 caagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat catgcccctt
      661 atgacctggg ctacacacgt gct
//
LOCUS       DAFCHQ010000222          656 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000222 DAFCHQ010000000
VERSION     DAFCHQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.231"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LNC71_002969"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LNC71_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBK6467942.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>656)
                     /locus_tag="LNC71_002970"
     CDS             complement(481..>656)
                     /locus_tag="LNC71_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467943.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAFCHQ010000223          653 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000223 DAFCHQ010000000
VERSION     DAFCHQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.232"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(246..446)
                     /locus_tag="LNC71_002971"
     CDS             complement(246..446)
                     /locus_tag="LNC71_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBK6467944.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          245 a          100 c          107 g          201 t
ORIGIN      
        1 atcacctcga aaagagtata cgaacaaacg ttctttttgt aaaggtggaa tttattatac
       61 taaataaata ggtgaacaag tacttgtgcc aaattatgtg ccaaaaaaaa tcgaatttaa
      121 taaaacacaa acaaaaagaa atcctattat gataacattt cttataataa caaacacaac
      181 agaagacgtg taatagttag tcaggaacgt acaaataacc cctgtatcct ttgcggtaca
      241 gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc
      301 gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt atgtcttagt
      361 ccatcaataa acagtcgaag agaaaatagg gaaaaattga ttactaaaga aaaacttact
      421 aggtcgacgg tgtagttgat tttcatactt caatagattc gaattaaacg atttcgattg
      481 gcttatacca gtcctatcca acgaaagata tatcgttctt aactctagcg gtataacttg
      541 aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat ttttagtaat
      601 agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa aaa
//
LOCUS       DAFCHQ010000224          642 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000224 DAFCHQ010000000
VERSION     DAFCHQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.233"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(91..>642)
                     /locus_tag="LNC71_002972"
     CDS             complement(91..>642)
                     /locus_tag="LNC71_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HBK6467945.1"
                     /translation="DRTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYS
                     CGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGC
                     PYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          180 a          151 c          120 g          191 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tc
//
LOCUS       DAFCHQ010000225          631 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000225 DAFCHQ010000000
VERSION     DAFCHQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.234"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            38..616
                     /locus_tag="LNC71_002973"
                     /pseudo
     CDS             38..616
                     /locus_tag="LNC71_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          212 a          113 c          126 g          180 t
ORIGIN      
        1 ttaatttttc tgtctagtta attgtagcct atccacagga atcaaatcgc atttatgggt
       61 aacacaaaat atttggggcg atcatacaga tccttgcggt tatctttctg aaatgggtgt
      121 aagtaaagaa aaactagcct atgatttggc tcatggtttt acggatgata atccaactac
      181 ttcggatgat aatccaacta cttcggagga taaaccagta attgatccaa ctcgagcagg
      241 tgctgcaaat tctacgctga cagatggaac aaattacgcc cacgttgatc agttcggaga
      301 aatcgaaaac gcaaacttgc atgtagctgg atggcacatt gctaattata aatacgagta
      361 tattttcatt atggactaca atactggaaa agaactagct agagtaagag ctgatgggat
      421 ttatagacca gacgtaaatc aagcttataa tactttagga aatgttggct atcatgtatc
      481 tttcaatatg cgtgatttcc ctaataagaa agtatacgtc atgatgcgtg cgacaaacga
      541 tccagaagga aatactaagg gtggagcaca agattttcat gacaagcgtt ggtatttaaa
      601 tattccacaa cgataaaaaa ttcccccctc t
//
LOCUS       DAFCHQ010000226          617 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000226 DAFCHQ010000000
VERSION     DAFCHQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.235"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..>617
                     /locus_tag="LNC71_002974"
     CDS             22..>617
                     /locus_tag="LNC71_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBK6467946.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEF
                     GHLEGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAV"
BASE COUNT          218 a          117 c          129 g          153 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaagattat
      481 gtcgttttcg aagaagagtt tggacattta gagggtgata cgattgtcgg catccaccat
      541 aaaagtgccg tcatcacact cgttgagcga ctttcaaaag ccattatcgc cttgaaacca
      601 gaaggccgta aggcagt
//
LOCUS       DAFCHQ010000227          601 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000227 DAFCHQ010000000
VERSION     DAFCHQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.236"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            237..>601
                     /locus_tag="LNC71_002975"
     CDS             237..>601
                     /locus_tag="LNC71_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBK6467947.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFH"
BASE COUNT          242 a           67 c           85 g          207 t
ORIGIN      
        1 tttggagggg cggtacataa aaatttatta tttagattct ataaacatgt gcaacttatc
       61 taacaacaaa gtatatattc tacagaagta aacaaatagc ttatatttta ggatgtacac
      121 aaattgacaa gtataataaa actttgataa aatacaaatg ttttttattt ttatgtgtga
      181 ttagttagaa cttacttaaa tcctattata atatttttgt taatgatttt aataaaatga
      241 ttggagagaa aatgatggat caaaaagcgt ttatcgaatc tattgattct acaatcgata
      301 gattaaaaaa agaaattcga ttttataata gagtaattgg tatcggaaat attataaaaa
      361 tagtcttatc agctagtatc ccaatattaa ttgatcaggc ttccgaacac aggtctttac
      421 tattgattgt ttcaattgct tcagcaataa ttacaattat acaaagtggg atgtctgcat
      481 ttaattatca agataaagta cagacttcga cagagttatt aatgaaaatt gaaaaagaaa
      541 agttgttata tataacgaaa acatccccat ataataaaac agatgaagaa aatttccatt
      601 t
//
LOCUS       DAFCHQ010000228          539 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000228 DAFCHQ010000000
VERSION     DAFCHQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.238"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>539
                     /locus_tag="LNC71_002976"
     CDS             <1..>539
                     /locus_tag="LNC71_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBK6467948.1"
                     /translation="QQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQE
                     YGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQ
                     AAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQD
                     HRPVKRRNKFYRSLRTASTT"
BASE COUNT          186 a           97 c          104 g          152 t
ORIGIN      
        1 cagcaggatg tgattattgt agccgtgggc tactatcttc gttataacct tagctatcgt
       61 gaagttcaag aaatcttata tgatcgtggc attaacgttt ctcatacgac gatttatcgt
      121 tgggtgcaag aatatggcaa actactctat caaatttgga aaaagaaaaa taaaaaatcc
      181 ttttattcat ggaaaatgga tgaaacgtac atcaaaatta aaggaaaatg gcattatttg
      241 tatcgagcca tcgatgcaga tggtttaacc ttggatattt ggttacgtaa aaaacgggac
      301 acacaagcag cctatgcttt tcttaagcgg ttagtgaagc agtttgatga accgaaggtt
      361 gtagtcacag ataaagcccc ctctattaca agtgccttta agaaactaaa agaatacggc
      421 ttttatcaag ggacagaaca tcgtaccatt aaatacctga ataatttgat tgaacaagac
      481 catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc ctctaccac
//
LOCUS       DAFCHQ010000229          531 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000229 DAFCHQ010000000
VERSION     DAFCHQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.239"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            71..>531
                     /locus_tag="LNC71_002977"
     CDS             71..>531
                     /locus_tag="LNC71_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBK6467949.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          198 a           73 c          108 g          152 t
ORIGIN      
        1 ggtagtaact aaaaataaat atgttataat agtatcaaat gaatattgct aaaagaaaag
       61 aggtaggaac gtggtagcga agaaatatgt aatcggtaat tttaaaggtg gcgtaggtaa
      121 aagtacgtgc gcgcaaatgt tagggtttga atcagcagtt aatgaaggaa aaagaacctt
      181 gataattgac ttggatatgc aaggaaatac ttctgatgtg atgaacttaa cgcatatgaa
      241 cttcactaac gaagaaggag gcggagaagg tgaaccgatt gtttacgaaa atactgtgac
      301 tgacgtgttg ataaacaatc ttccacctaa agaggctgtc tataaagtaa tcaataacct
      361 ctatatactt ccagcagaca tgtcctttga aatgtatgat gattggatca aagaccaata
      421 taatgattca ttatcccaat tcaaatatat gaaagaaaag ctagaaccat tatttgatga
      481 gtttgacgtc atctatttag atgtcccacc ttctatttct gtatatagta a
//
LOCUS       DAFCHQ010000230          526 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000230 DAFCHQ010000000
VERSION     DAFCHQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.240"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..354
                     /locus_tag="LNC71_002978"
     CDS             <1..354
                     /locus_tag="LNC71_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBK6467950.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..>526
                     /locus_tag="LNC71_002979"
     CDS             355..>526
                     /locus_tag="LNC71_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467951.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAFCHQ010000231          512 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000231 DAFCHQ010000000
VERSION     DAFCHQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.241"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(30..332)
                     /locus_tag="LNC71_002980"
     CDS             complement(30..332)
                     /locus_tag="LNC71_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467952.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          176 a           90 c           70 g          176 t
ORIGIN      
        1 tccgggctac cttttttagg tagcctactt taatctttat acctttctgg atcaacgaaa
       61 gtatacttca aatagtcata acgccgatga tcgctccgtg cgtctggcac atcagtcacg
      121 atatcaaaca aaaaatacac atctttcttc attctggttt ttgttgccgg aattttaaag
      181 tagttcttat tagagtaata gagattgatt aataagctat cttcgattgc taaaaagaaa
      241 acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta
      301 aaccataatt gtgtttttcc ttccatcaac ataatgttca cctcaaaaag agtatacgaa
      361 ctaatgttct ttttgtaaag taggaatatc attgataata tggatggaac atatatatta
      421 aaaagtgttc tttttgaatg ctattttgaa tgccattttc attcatttaa cgaaaatctg
      481 cgaaaataaa aaatgctcaa acccttataa aa
//
LOCUS       DAFCHQ010000232          510 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000232 DAFCHQ010000000
VERSION     DAFCHQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.242"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..508
                     /locus_tag="LNC71_002981"
     CDS             <1..508
                     /locus_tag="LNC71_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HBK6467953.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          155 a           90 c          110 g          155 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact
//
LOCUS       DAFCHQ010000233          508 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000233 DAFCHQ010000000
VERSION     DAFCHQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.243"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..343
                     /locus_tag="LNC71_002982"
     CDS             <1..343
                     /locus_tag="LNC71_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBK6467954.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          204 a           69 c           93 g          142 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggcccta
//
LOCUS       DAFCHQ010000234          501 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000234 DAFCHQ010000000
VERSION     DAFCHQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.244"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            141..398
                     /locus_tag="LNC71_002983"
     CDS             141..398
                     /locus_tag="LNC71_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467955.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          216 a           66 c           69 g          150 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaaat tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 acaaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa t
//
LOCUS       DAFCHQ010000235          462 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000235 DAFCHQ010000000
VERSION     DAFCHQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.246"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..430)
                     /locus_tag="LNC71_002984"
     CDS             complement(<1..430)
                     /locus_tag="LNC71_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBK6467956.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAFCHQ010000236          437 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000236 DAFCHQ010000000
VERSION     DAFCHQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.247"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..437
                     /locus_tag="LNC71_002985"
                     /pseudo
     CDS             1..437
                     /locus_tag="LNC71_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          179 a           77 c           80 g          101 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattga
//
LOCUS       DAFCHQ010000237          429 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000237 DAFCHQ010000000
VERSION     DAFCHQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.248"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            83..412
                     /locus_tag="LNC71_002986"
     CDS             83..412
                     /locus_tag="LNC71_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBK6467957.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          166 a           50 c           77 g          136 t
ORIGIN      
        1 cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag taattacata
       61 ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca attcaatcat
      121 gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag caaagttggt
      181 agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg attttagcga
      241 agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc ttgtagaatt
      301 taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag atacacttac
      361 taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat gaaatcgttc
      421 cttcaaaac
//
LOCUS       DAFCHQ010000238          404 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000238 DAFCHQ010000000
VERSION     DAFCHQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.denovo.249"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>404
                     /locus_tag="LNC71_002987"
     CDS             <1..>404
                     /locus_tag="LNC71_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467958.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAFCHQ010000239        10793 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.guided.085, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000239 DAFCHQ010000000
VERSION     DAFCHQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.guided.085"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..998)
                     /locus_tag="LNC71_002988"
                     /pseudo
     CDS             complement(1..998)
                     /locus_tag="LNC71_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            1489..2427
                     /locus_tag="LNC71_002989"
     CDS             1489..2427
                     /locus_tag="LNC71_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBK6467959.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2557..3204
                     /locus_tag="LNC71_002990"
     CDS             2557..3204
                     /locus_tag="LNC71_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HBK6467960.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3897..4835)
                     /locus_tag="LNC71_002991"
                     /pseudo
     CDS             complement(3897..4835)
                     /locus_tag="LNC71_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4835..5452)
                     /locus_tag="LNC71_002992"
     CDS             complement(4835..5452)
                     /locus_tag="LNC71_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBK6467961.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5466..5636)
                     /locus_tag="LNC71_002993"
     CDS             complement(5466..5636)
                     /locus_tag="LNC71_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467962.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(5984..7474)
                     /locus_tag="LNC71_002994"
     CDS             complement(5984..7474)
                     /locus_tag="LNC71_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBK6467963.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(7517..7816)
                     /locus_tag="LNC71_002995"
     CDS             complement(7517..7816)
                     /locus_tag="LNC71_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467964.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8182..8466)
                     /locus_tag="LNC71_002996"
     CDS             complement(8182..8466)
                     /locus_tag="LNC71_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467965.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(8985..9470)
                     /gene="vanZ"
                     /locus_tag="LNC71_002997"
     CDS             complement(8985..9470)
                     /gene="vanZ"
                     /locus_tag="LNC71_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBK6467966.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(9623..10534)
                     /locus_tag="LNC71_002998"
     CDS             complement(9623..10534)
                     /locus_tag="LNC71_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBK6467967.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3335 a         1878 c         1671 g         3909 t
ORIGIN      
        1 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
       61 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
      121 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
      181 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
      241 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac aagcttattc
      301 ttaatcactt ggatatattc tttttgtttt aacgcttcaa taatacttgc tcttgtcgct
      361 tctgttccaa tcccctcaac ttcttgtaaa atcttgattg cttcttcatc atcaaccgtt
      421 ttattcgccg ttttcatagc agttaataat gtaccctctg taaaagcttt tggcggttgc
      481 gtttcttttt caacactctt aacaccaact tcggcacgtt cgccaatgat aacgagtggt
      541 aacgtttgaa cgtcctcttt ttcttctttg gtttgttgtt tgaaaagaat tttccaccct
      601 tcttgctttg gtgtctttcc gatcgattga aacagcaaat cagctacttt cgtttgtatc
      661 ttggtttctt catacaagta atcaggtaaa aacatggcaa cggtcgtttt aacgactaaa
      721 gcataaattt ttcgttgtaa atcgtccatt ttcgctaatg cagattcggt aggtacttgt
      781 tttgttggga taatggcgtg gtgttcctgt accttactac cgtccacata acgctttcta
      841 ggctctgttt gaaccatttc aagatcaagt cctaaaaaac cgctatattt gccaaaattc
      901 gcttttaaat aagcaaattc gttctctgta ataaatggtg tatctgttct tggataactc
      961 aataattttg cttcatacag tccttgcata gcttttaaac gtaaaatttt gcgttctaag
     1021 acgttttaaa tatatctgcg tatattttta tgtgttttat cttttcgtcg gcaaatgcgt
     1081 taaaatttgc gaaataaaat agctcaaggg cgaacgcagt gagtgcattt acccttgatc
     1141 tatttttttg agtaaattag cttttgaaac cgacggaaag gaaacacaaa acttttcaga
     1201 ggcaaggggt ttttaagggg cgcttttagc cccttgaatt tatttgcttt aagatatatt
     1261 tagttttaaa atagttttaa cgaaaaaaag acataaaaaa agacgagcaa cgctaccaac
     1321 gttctcgccc ggaataacaa accacacttg ttattcattc gatgattagt atatcaattt
     1381 ttgattttga atacaaaaca aaatggacga atggatacag gcgaaattta ttttgcgatt
     1441 ccactcgtcc ttttttcgtt ccaaaaatta ttttaggagc gataaaaaat gaagaaaatt
     1501 gatgaaaaat tagctgcaga aatctcacta aaaaaaccgg ccgtctctaa tagccggtta
     1561 agtaatagcc ggttaagtgg tcaaactttg ggaaaatctc aacccgcatt aagttttgat
     1621 gccatgacaa tcgttggaaa tttgaacaaa actaatgcta aaaagctatc tgactttatg
     1681 agtgtagagc cacaaatacg actttgggat atacttcaaa caaagtttaa agctaaggca
     1741 cttcaagaaa aagtttatat cgaatatgac aaagtaaaag cagatacttg ggatagacgt
     1801 aatatgcgtg ttgaatttaa tcccaataaa ctcacacatg aagaaatgat ttggttaaaa
     1861 caaaatatta tcgactacat ggaagatgac ggttttacaa gattagactt agcttttgat
     1921 tttgaagatg atttgagcga ttactatgca atgactgata aagcagttaa gaaaactgtt
     1981 ttttatggtc gtaatggcaa gccagaaaca aaatattttg gtgtccgtga tagtgataga
     2041 tttattagaa tttataataa aaaacaagaa cgtaaagata acgcagatgt tgaagttgtg
     2101 tctgaacatt tatggcgtgt agaagttgaa ttaaaaagag atatggttga ttactggaat
     2161 gattgtttta atgatttaca catcttgaaa cctgcgtggg ctactttaga aaaaattaat
     2221 gagcaagcta tggtttatac tttgttgcat gaagaaagta tgtggggaaa gctaagtaag
     2281 aatactaaga ctaaatttaa aaaattgatt agagaaatat ctccaattga tttaacggaa
     2341 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaagcagat tgatttttgg
     2401 caacgtgaat ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca
     2461 gtataattaa tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct
     2521 aaatataaaa atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta
     2581 gaaaattggg atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc
     2641 attactaaag aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa
     2701 atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga
     2761 acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca
     2821 gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc
     2881 atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg
     2941 tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat
     3001 tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg
     3061 ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat
     3121 cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt
     3181 catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac
     3241 gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt
     3301 tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg
     3361 cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt
     3421 ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag
     3481 caaaagattt taaagagtta ttttatttta agtctttata acatgagtga agcgaatttt
     3541 taaatttcga tagaaatttt tacatcaaaa agccccctgt caaaattgac gaagggggtt
     3601 ttttggcgca cgcttttcgt tagaaatata caagattgaa aatcgtgtat aagtgcgccc
     3661 tttgttttga acttagcacg ttacatcaat tttttaaaat gatgtataag tgcgcccttt
     3721 taaattttga gtgattatat tttttgagtt agaaaaaggg attgggaaaa tttcccaaaa
     3781 taatttaaaa aataagcaaa aattttcgat agagaatgtg ctattttttg tcaaaggtgt
     3841 ataccttgac tgtgcttgct gttacattaa gtttattttt aagttattaa aaaagaaata
     3901 gcttttaaag tttggctcgc tgtcgcttta taaagctgat tgacttttga ttgcaaacta
     3961 cttaaagaaa acaaactcgg actattcgtt ttcttctctt tggtttgaac atcagcaatt
     4021 atcccctctt gattgcctat tttagcttgt ttagaagaaa caaaagctaa aagctcctct
     4081 tgggttttaa aacgctgtgt ggggcttaga acgcccttaa acgacccttg gtttactttt
     4141 atactagctt ccacctcgaa aaaaggttct tttttaaaat tctctatggc ttcctggcgc
     4201 tgaaaaataa ggtataaggt gggcgtttga acacgtccta gtgaaaatgt accttgtacg
     4261 cccttctgtt gtaaatttaa cgtatacaaa gggcttgcgt tcatgccgat caaccaatcg
     4321 gcaatttggc gtgtttgcgc ttcttgataa aagggatagt aattcattcc aggttgcaaa
     4381 ttttgaaaac cgcttcggat tacatctttt tctaagctat tgatccatag tcttttaaat
     4441 gttttatctt ttgaaaaggc atttgcttta tggataatcg accaggcgat attttcacct
     4501 tctctgtcgc tatctgttgc aacaataatt gtatttgcct ttttgagaag ttctgcaaca
     4561 attttaaact gctttccctt atcttttgca acttcaaaat cgtatcgatc aggaaaaatc
     4621 ggcaaagatt caagtttcca attttgccac ttttcgtcat aatgacctgg ttctgctaat
     4681 tccactaaat gcccaaaacc aaaggtgata aacgtttcat ctgtaaatag tgggtctttg
     4741 atctcaaaat aaccgtcttt tttggtgctt tgttttaaag cacttgcgta ggctaatgcc
     4801 tggcttggtt tttcagctaa aataaccgta ctcattaact atccctcttt tcattgtttt
     4861 ttctttgatc gactgtcacg ttatatcttg ctcgatacct tctaaacgtt cggcgattga
     4921 ttccagtttg ttcttcaact tctttatcgg ataaaccatt caaaaacaaa tcgaaagcat
     4981 gctttaaacg tggatcattt tctttaaatt tatgctgacg acctttgaat ttgccttttt
     5041 tcttagcaat ttcgattcct tgtgcctgac gttccttaat tttttttcgt tctgattctg
     5101 cttgatactt gtacaattca atgacaaggc tattaatcaa acgccttaaa ttttcatctt
     5161 caataccatt cattgagggt aaatttaaga cttccagggt tgccccctta atttgaattt
     5221 gattcatcaa ttctgttaat tctttattat ttcgtcctaa tcgatctaat tcagtaacaa
     5281 taacaatatc cccttcacga atatagttaa gcatagcttg taattgtggg cgttcgaccg
     5341 attgaccgct taatttgtct gaaaagacct tagaaacgcc ctgtaacgct tgtaattgcc
     5401 gatctaagtt ctgttctttg ctactgacac gtgcataacc aattttagcc attttcaacc
     5461 aacctctaaa attctctcgg ttgcaataac caatcagcaa tatctacttt ttcaatttca
     5521 aattgcttat cagaaattgt cttttcgtaa gcgataaaat cttgcgcata ttgttgctca
     5581 ttaaaaatag ccaccacttc gtcattttct aaaactcgat aaataaattt tttcatttta
     5641 ctcctcctat tatgcccaac ttaaatgacc tattcaccaa gtcaattata ctgctaaaat
     5701 catattagga caaataggta tactctaatg acctataaaa gatagaaaat taaaaaatca
     5761 agtgttcgct tcgctctcac tgcccctcga cgttttagta gcctttccct cacttcgttc
     5821 agtccaagcc aactaaaagt tttcgggcta ctctctcctt ctccccctaa taattaatta
     5881 aaatcttact ctgtatattt ctgctaatca ttcgctaaac agcaaagaaa aaacaaacac
     5941 gtatcataga tataaatgta atggcatagt gcgggtttta ttttcagcct gtatcatagc
     6001 taaacaaatc gagttgtgtg tccgttttag ggcgttctgc tagcttgttt aaagtctctt
     6061 gaatgaatgt atgctctaag tcaaaagaat ttgtcagcgc ctttatatag ctttcttttt
     6121 cttctttttt tactttaatg atcgatagca acaatgattt aacactagca agttgaatgc
     6181 caccatttct tcctggttta atcttaaaga aaatttcctg attcgccttc agtaccttca
     6241 gcaatttatc taatgtccgt tcaggaatgc ctagcacttc tctaatctct tttttggtcg
     6301 tcactaaata aggcttgtat acatcgcttt tttcgctaat ataagccatt aaatcttctt
     6361 tccattctga caaatgaaca cgttgacgtt cgcttctttt tttcttgaat ttaaaccacc
     6421 cttgacggac aaataaatct ttactggtta aatcacttga tacccaagct ttgcaaagaa
     6481 tggtaatgta ttccctatta gccccttgat agttttctga ataggcactt ctaacaattt
     6541 tgattacttc tttttcttct aagggttgat ctaatcgatt attaaactca aacatattat
     6601 attcgcacgt ttcgattgaa tagcctgaac taaagtaggc taaagagagg gtaaacatga
     6661 cgttattacg ccctattaaa cccttttctc ctgaaaattt cgtttcgtgc aataagagat
     6721 taaaccaggg ttcatctact tgttttttgc cttctgtacc gcttaaaacc gttagacttg
     6781 aacgagtaaa gcccttatta tctgtttgtt tgaaagacca atcttgccat tctttgaaag
     6841 aataacggta attaggatca aaaaattcta cattgtccgt tcttggtatg cgagcaatac
     6901 caaaatgatt acacgttaga tcaactggca aagactttcc aaaatattct cggatatttt
     6961 gcgaaattat tttggctgct ttgacagatt taaattctga ttttgaagtc acatagactg
     7021 gcgtttctaa aacaaaatat gcttgataac ctttatcaga tttgataatc atagtaggca
     7081 taaaacctaa atcaatagcg gttgttaaaa tatcgcttgc tgaaatagtt tcttttgccg
     7141 tgtgaatatc aaaatcaata aagaaggtat tgatttgtct taaattgttt tcagaatgtc
     7201 ctttcgtgta tgaacggttt tcgtctgcat acgttccata acgataaacg ttgggtgtcc
     7261 aatgtgtaaa tgtatcttga ttttcttgaa tcgcttcctc ggaagtcaga acaacaccac
     7321 gaccgccaat catgcttgat tttgagcgat acgcaaaaat agcccctttg cttttacctg
     7381 gcttggtagt gattgagcga attttactat ttttaaattt gtactttaac aagccgtcat
     7441 gaagcacagt ttctacaaca aaagggatat tcattcagct gttctccttt cctataaatc
     7501 ctataaaata ggttgtttaa ttaacttggt ttgctttttc attcaactgt ttcaatattg
     7561 catgttttga aaaagatttt tttcctttat aagtcaattt ttttccacta atcgaataaa
     7621 ttattttgtt attttctatt aacttatata tataatcttc cccctccgaa gaaaaatact
     7681 tatctgattt tgtttctaag tagatatttc tcttttctaa ctctttctta aacgtttcta
     7741 gtgtatagat atttgctaat tttcttatct ccaataaact attttttata taagttttac
     7801 attcatcatg attcatacaa actccacctt ctataaatga atacaaaaaa agcaatcaaa
     7861 cgatttccga ttgattgctt aacaattctt aaattcagta gcttagatac ttgaaaactc
     7921 tctgatttcc ctatataatg atagtacggt tatataccgt cttcaaacaa agttaattaa
     7981 ataacttctt acgagggaag agttcatctg actaactgat aagcgttggt ttggcaatct
     8041 tatcgggcta tgcatttata aaatgtcgtc aaacatttta taaatgtgtc atggctcttt
     8101 tttcgtttct attcagttcg ttgtttcgtt atatctagta taccgctttt aaaaaaaata
     8161 agcaacgatt tcgtgcatta ttcacacgaa gtcattgctt ttttcttctt ccatttctaa
     8221 atccaatgtt acttgttctg attctgtttc tggttctggt tctgttggct catttgggat
     8281 taaatccact actagcgttg agttagttaa ctttgcaatt tgttctagtg tttttatggt
     8341 tggatctgat tttcctgatt ctattcgtga ataatttgat ctactcattt ctaattcttg
     8401 ggagaatttt tcttttgatt tggtacctct catctttttt aagctttctc taaatgctag
     8461 ttccaagttt ctcccccctc tttttgtgta ataaaattca cgctttattt aaattatagc
     8521 ttttaatatc ttaataaaca agtgattaat cacttgtgat ttgtgattat tccaaaagta
     8581 ggtaatttca ccaacctttt tcaaaagttt ttatcgacga ttattggact ggatcgtgtt
     8641 tcctaaagcg attctaagcg catgtttaat tttaaagtgg gtaattctct ttaaaacgtc
     8701 ctacaaagat tcaaaaaaag catggctgct agtgggggta ccgtcaggaa aatgcggatt
     8761 tacaacgtta aggaaattta ttaaagagac caattcctca gcactaaaat tgataattgg
     8821 gctttctttg ttactatagt gttaggtaag ctttttactt tatcaccggt tttcccctca
     8881 cttcacacct acgggcgagt ttccccacat cccgttttcc atctaatcca attcattcaa
     8941 tatctttatg tgtatgaaag atcagattgc ttgatttaga catcttacac gtaatttatt
     9001 cttaaatggg tacggtaaac gagcaataat acgagcaaaa gtatacctac aaaaataata
     9061 actctgtcta attttttttg attcatatgc ttattgctta aaccatataa tttcagtcca
     9121 agaaagcctc caacagtatt tgtaattaca tctgttatgt ctgtcgctcc aatagcgaag
     9181 ataaattgaa ttatttcaaa agtaagactt aaaaccagta caaaagcaaa cttaggtaaa
     9241 aatccgattt ctttaaaatt gacattcaaa agcaagccaa atggaataaa gattataaca
     9301 ttatctatca tctctctgaa attcccagta gcagtaaatg gagtcaagtt aagacttctt
     9361 tgatgataat taaatactga taaaatattg tattgtaatt tgaataacac taaccagatt
     9421 agtgtcacta aatataaagc tagcaatcct ctagataata tttttcccaa tataatttcc
     9481 tctaattctt ttgtttaagt tgtatcatta gtatagcaat gtcctagtta acgtgggttt
     9541 taaattaaaa agctaacgtt accccaaaag tgctagtaca ataatgctgt tcattaattt
     9601 cgttggtttc attccgccat ccttacctcc ttgaattagt atgtgttgat ccgggaaaca
     9661 ctgtcacaat tacaccgtct atattgtttc ctgatatctc ataacgaaga ttagtcggca
     9721 catgaatggt ggtattttta gtaacaggat aataaaagat ctcatatttt tccccattta
     9781 cactaacaga aatggttttt tcttctttta ggtaatccat atattcctcg agaacgaaat
     9841 tcttttcttt cataatcgca ctatgtggta aaccaacata gcgaatatgc catggttcat
     9901 attgaattcc tgttaactct gttttgtcct ctggataacg taaaatgaac ccgtatttcc
     9961 aagcattttc ttctatccac tttccttcag gggctcgttc cattttcgtc aagcttgatc
    10021 ctacatctag tgataaacct gaattatgct cactataacc tgctggtaag gcatactcag
    10081 cccccatttc ttggtaaagc acactttgct catcaaagtc tcgatagcca ctattaataa
    10141 taaaatgact aacgccaccc tttacagcat cattgaccat ctctgaaaat ttttgtgcta
    10201 tttcttttga catataaata ttactatcaa gcaacccgta tccatttatt aattcgtcat
    10261 gtttagataa attcacgata tctgacttca cactttcttg gcgaacagga tatttactat
    10321 tgattaatag cagatttcct tgataaacct gttcttctgt aattgttttc tcttgggtat
    10381 tttcagaagt cccactattt tctaaatgtt ctttgggatt ttgatcataa ttttgaaatt
    10441 cgactttttc ctgagaaaac agtgcttcat taacgtagtc ataacctaag tatattaaga
    10501 ataacaataa caataaaaaa aacaacttct tcattttcag tctcctcctt aactatatag
    10561 ttggaagtat gaaatatttc tagaatcttc cccttaaacg gcacaatctc gtttgagatt
    10621 gtgccgtttt gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt tactattatc
    10681 accaatgtag atgaattttc ataagtgata cccatgctga atttaacgag gctgaactgc
    10741 ctgttgacaa tgggggttct gttgcaaagt tttaaatcta ctatcaaata agg
//
LOCUS       DAFCHQ010000240         9901 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.guided.091, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000240 DAFCHQ010000000
VERSION     DAFCHQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.guided.091"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(61..933)
                     /locus_tag="LNC71_002999"
     CDS             complement(61..933)
                     /locus_tag="LNC71_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBK6467968.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1061..1630
                     /locus_tag="LNC71_003000"
     CDS             1061..1630
                     /locus_tag="LNC71_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBK6467969.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1697..2434
                     /locus_tag="LNC71_003001"
     CDS             1697..2434
                     /locus_tag="LNC71_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBK6467970.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2665..3933
                     /locus_tag="LNC71_003002"
     CDS             2665..3933
                     /locus_tag="LNC71_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBK6467971.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4420..4884
                     /locus_tag="LNC71_003003"
     CDS             4420..4884
                     /locus_tag="LNC71_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBK6467972.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4868..5497
                     /locus_tag="LNC71_003004"
     CDS             4868..5497
                     /locus_tag="LNC71_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBK6467973.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5851..7329
                     /gene="msr(C)"
                     /locus_tag="LNC71_003005"
     CDS             5851..7329
                     /gene="msr(C)"
                     /locus_tag="LNC71_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBK6467974.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7376..7822
                     /locus_tag="LNC71_003006"
     CDS             7376..7822
                     /locus_tag="LNC71_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBK6467975.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8021..8536)
                     /locus_tag="LNC71_003007"
     CDS             complement(8021..8536)
                     /locus_tag="LNC71_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBK6467976.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8653..9855
                     /locus_tag="LNC71_003008"
     CDS             8653..9855
                     /locus_tag="LNC71_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBK6467977.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3151 a         1743 c         2063 g         2944 t
ORIGIN      
        1 ggatcttttg cttaacttat tcgttgttaa aaagctgaaa gcgaaggaaa aaaactctac
       61 ttaactgtat ttctccgtta ggacatctaa aaatttacga gcagcaggag aaagaggctg
      121 gtcttttttc caaatgagat tcagtgaagc ttgtaatggc ggatcgagtg ggatgaatac
      181 tttttccgtc ccataagtat tgatgatccc atctagagct aatgcaccaa aagtcccgtt
      241 atcgaccatc aaagaagcat tgtacaataa gttatatgtt ccgacgatcg ataactgatc
      301 tatgctgaaa cctagccatt cagctagttg actgtccact gacgtttgcc gggaaatgat
      361 caaaggaaca gttttcagtt cctgcggatg gatgactgtt tgctttgcta aaggatgatt
      421 ccgatgtatc aaaagtcccc aacgatcttg ggctggcagt cgaaatgact ggtatttttg
      481 tttgtttgtc ggttcgatta cgagtccaaa atcaagtaat ccgctatcca gtttgtctat
      541 gatatcatct gcattcccgc tatataaatg aaactggata tcaggatgcg ccttgatcaa
      601 cgaatgaagg ctatcagaaa tgaaagagat tgctttagtt tcgcctccgc caatgtagat
      661 ttctccagag atgattgcgt catttgttaa attgttcgcc gttttttcta ccaacgacaa
      721 gatttctttt ccttttgaat aaagatatcg accagaatcc gtcaactgta tctctcggga
      781 gcctcgagtg aaaagtgtca caccaagttc ttcttctaaa tctttcagtt gttttgagag
      841 agtaggttgc gtgatatgca gagattttgc tgctcggctg atattttttt ctctagctac
      901 tgctaaaaaa tacgtcagta tacgaatatc cattttttcc ctcctgtttt taaaaaagca
      961 taccactaag tatagttaaa aggaatagtt aagtatgttt tataagtatt agacatcata
     1021 agccgtccgt tataaagtaa ggataaaagg agggagcaaa atgacaagcc tcatcgaaaa
     1081 acaaatcgtc aataaagaga ttcgagtaac ggatccgctc tttgaagtga tccatcagat
     1141 ccaagcggaa aacgaagagc ctttagcagc gctgaatact gggtaccatg aaccagcaga
     1201 tatccgcaaa cggctagaaa atatcattag tgacaaagta gatgatacgg tgactgttct
     1261 gctgcctttt tatacagatt tcggcaaaca tattagtata gggaaaaatg tctttatcaa
     1321 tcgtcaagtg atgttcgttg acttaggtgg catctgttta gaagattcag tattgattgg
     1381 tcctcgtgtc aacctcatca cggtgaacca tctaaccgat ccaaaagaac gaagaggctt
     1441 gtcagtaaag ccaatccaca tcaagaaaaa tgcttggatc ggagcaggag caactattct
     1501 gccaggcgta acgattggcg agaatgccat tgttgcagcg aatgcaactg ttacgaaaga
     1561 tgtcccagac aataccatcg ttgcaggaat ccctgccaaa atcgtcaaac ctgtcgaacg
     1621 gattatttga acagtaagat gaaataagag atagtgagta taagcaagag atttttcaaa
     1681 acaggaggag aatagaatga atgatttatc agaaaaagtg attgtcatca tgggcgcttc
     1741 aagcggaatc ggtgaagcaa caacgaagct attagcagaa aaaggggcta aactagttat
     1801 agctgcacgc cgagaagatc gcctaaaagc aataaaagaa tctttaccag aagctgagct
     1861 atatattcaa acagcagatg taagagattt tgcacaagtc caagcagtta tcgatttagc
     1921 gatggaaaag ttcggtcgaa tcgatgtatt gtacaataat gcaggaatca tgccaaccgc
     1981 tccattagtt gaaggtcacc gtgatgaatg gcaaaatatg cttgatatca acatcatggg
     2041 tgtgttaaat ggaatttccg ccgtacttcc aatcatggaa aaacaaaaat ctgggcatat
     2101 catttcaact gattctgtgg cgggacatgt tgtttatcct gattccgccg tttattgcgg
     2161 aacaaaattt gctgtccgtg caatcatgga aggattacgt caagagcaac ggcaaaataa
     2221 catcaaatct acgatcatct ctccaggagc tgttcaaact gaattatatc aaaccatcag
     2281 cgacaaaaag gctgctcttg aacttcatga agcgcaaaaa gaatggggat taacatctga
     2341 agatatagct tctgctgtag catttgcgat agaaacgcca gaccggatgt ctgtcagcaa
     2401 catgattatc cgtccgacga cacaggaagt ataatccata acggataata tccagagaga
     2461 attacttgtt aaatagttat tcaaacgacc atcaatttaa ggcgtgaatg gagaagaata
     2521 ttcttcattt acgtcttttt atgcagaaaa agcaaattta ttaaaaagaa acaaatgtta
     2581 tttaaatatg gacattgact tgcttttact tttttagtta cactgttatt taggaaaagt
     2641 ttcataattc aaaggagaaa aaagatgaaa gcaaagaatt tattatttag cgttattctt
     2701 ttaggaaatc tagcagttgc caacacaaca gtgctagctg aagaatcgac agaaactgtc
     2761 gcaactcaga catcgagttc ttcagaaaca ggaattgaaa acgatacaga aagtactttg
     2821 cagtcagcta ctgtctcaga aaatacaatt tcaaccgaag aaccaacaac tgaaacaaca
     2881 gaacaaacac aaaccgaaga cagtacatca gcagaagctc agaacaatcc agagaatgca
     2941 gaagaaatca cgaaccaatt gacagatggg caaacattga cacataccca tcaagcagat
     3001 ttgtctgaat tcgaaaacta tactggctat gctttttatg ctcgctcatc tgctaattct
     3061 caacaagcgt tcattgatag tattgcctca actgcacaaa gtttggcaag tgccaatgac
     3121 ttatatgcat cagtcatgat tgctcaagcc atcgttgaaa gtggctgggg gaacagtact
     3181 ttggcttctg cccctaatta caatctattt gggattaaag gaagctataa tggccaatct
     3241 gttacgatgc ctacttcgga atatgtgaat gggcaatgga ttacggtgaa tgccgctttt
     3301 agaaaatatc cttcgtacaa agaatcattg caagataacg taacggtatt gaagacgact
     3361 tcttttcagc caggcgttta ctactattcc ggtgcatgga aaagcaatac gaactcttac
     3421 aaagatgcta cggcttggct aacaggtcgt tatgccactg cgcctaatta tggttctaca
     3481 ttgaacaatg taatcgaaac ctataatctg acacagtatg atacagcacc aacttcaact
     3541 tctccgatgt atcgtctgta taaccgtcat acaggagaac atttatatac attgaatgcc
     3601 ggagaaaaaa attatctgcc aactgtaggc tgggaatatg aagggattgc ttggcaagca
     3661 ccaaacagcg gacaacctgt ctaccgttta tacaatcctt acagtggaga ccatcattac
     3721 acaatggcac aatctgagat caactcttta acaaaaatcg gttggcgtta tgaaggtcta
     3781 agtttttatt ctggtggttc caagccaatt tatcgactat ttaatccaaa tgaaaaaact
     3841 ggtacccatc actatacctt gagtgctaag gaaagagatt ggctaacacc gatgggctgg
     3901 agatatgaag gaataggatt ttatggttat taaaaagagg tagtgaaaaa gctgccctgc
     3961 atcaagtagt aagaacagcc aaacaaaaac agattttcat gttttttgtt tggttgaact
     4021 tattaccgca gatgcaagtt tttgaacacc gtttactcaa aaaaggttgt gacaacatct
     4081 tgtcataact tttttattac tgtttaagca tactgatcca atttctaata ttcgacggga
     4141 ttcttgcaaa aggcgatttc ggaaattttt ggcggtgtaa aattatctac tgcagaagag
     4201 ctatcttcta tagtttgata agaatcaata atcttggttt tagcgttgtg ctttctttat
     4261 cattttttcc gttcattcta caaaaaattt cttttctcta tacttttcta ttgagagcgt
     4321 tttttttatt acacagctga ttctttgact tttttctttt tttattctat tattgacaag
     4381 tcaatcgttg acgagtcaat tattagaaag gaggaaatta tgtctttaga agagattagt
     4441 caccttttat atcaaatcaa attagccgat caaaaaatcg tccagatctt tgaagaaaat
     4501 atcggtataa gtcttactag atacgagatc atgatgagac tgaaagaaga aaatccattg
     4561 ctacaaagca gtttgcaaga aaagttgcag attgatcaag gagcagttac tcgtcatctg
     4621 aaggtattgg aagcaaatgg ctatgtttcg cgtcagagaa atcctcagaa caaccgggaa
     4681 gtgtatgtag cgctaacaga aaaagcccag aagaaaatca agcattgtga aatggaccat
     4741 aaagatacag gttccgtgct atccccggct ttcagcgatg tagaaatgaa acaatttcta
     4801 acacttttag ataaattgaa tacccaactt tgcaatataa caacagaaac gaaagaggtt
     4861 aataataatg gaacaaaaaa atgatttttc aacaattatg actggtagaa gatccgttcg
     4921 cgtatatgat gaaacataca aaatcccaca cgaagaaatg ctggaaatga tcaaagaagc
     4981 gacgactgca ccttcttctg tcaatatgaa accatggcgc tttgtggtcg ctgaaagcga
     5041 ggaagcaaaa gaaatcttgc gtcctttgat ccgtttcaat acgagacaaa atgatacttc
     5101 ttctgcaatg gtcatgattt ttggcgatat gcaatgttac gaatatggcg aagaaatcta
     5161 caaccaagct tacgaatcag ggaagatgcc aaaagaagtc agagatcaac aattagctgc
     5221 gatcattcct tattacaaga gtttttcaag agaagaaatg aacgatgtag tcaaagtcga
     5281 ttctagctta gcggctatgc aattcatgtt agttgcgcgt gatcatggct atgaaacaaa
     5341 tccaattggt ggattcgaag cagatcagtt ggcagaggca tttggtttgg ataaagaccg
     5401 ttatgttcca gttattattt tatcaatagg aaaagcggta gaagaaggtt acgagtctgt
     5461 tcgtttagca ccagaaaaga tcactatgtt cgaataaaag gttttcagtt actattcagt
     5521 agctattgct ttttttgaat cgtgtgtggt aacctaataa aaaaggaggt cggaaaaatg
     5581 actgcatcga tgaaattacg tttcgaactt ttgaataaca actaaataaa ttcaaaagac
     5641 atgtataaac aacaatttca tggatggagt atgtccattt tttcgaacga cagtagaata
     5701 ggtgaaagct atgacagatt tggtgattta gtcatagtta tttcgttcag agtaggaaaa
     5761 gatgtgatgc agtttttttg caaacagatt tttctgctct tttttagtat cgataattgt
     5821 tgttttacat gaaataggag tgtaaaacat atggaaaatt tagcagtaaa tataacaaat
     5881 ctacaagtca gctttggcaa ccagctagaa ctatctattg attctcttcg tgtctatcag
     5941 caagatcgta tagggatcat aggagaaaat ggtgtcggta aatcgacttt gctcaaacta
     6001 atagccggtg aactttttcc cgatcatggg aaaatccaaa cagagatcac cttcaactac
     6061 ctgcctcaat taacctatct cgctgaagca aaggacctaa atttggaatt agccagtcat
     6121 ttccagttaa gactggaaga aacttcagag cggaaatgga gcggagggga agaacgaaag
     6181 atcgagttga tacgtcttct ttcttcttat gaacaaggga tgcttctaga cgagccaaca
     6241 acccatctag atagaaaaag tattgatcga ctgattgaag agcttcgtta ttattatggt
     6301 acgctggttt ttgttagtca tgatcgctat tttctagatg agctagcatc gaaaatctgg
     6361 gaagtaaaag acggagaaat ccgagagttt tcggggaatt atagtgccta tctcactcaa
     6421 aaggaattgg agaaaaagac tcagctacga gaagcagagt cgatcatgaa agagaaaaaa
     6481 cgattggaaa aatcgatcca agaaaagaaa aaacaagcgg aaaagttgga aaaagtgtcc
     6541 agtaaaaaga aaaagcaaca aatcagactg gatcggttgt cttcctctaa acaaaaagac
     6601 agtgtacaaa aagccatcca aaagaatgcg aaaacattag agagaagact ccaaaaaata
     6661 ggagaaacaa ccaagccgca gcagatgaaa caaatccgtt ttccagtacc aaaatctctt
     6721 gaactccaca gccgctatcc catcatggga caaaatgtcc aattggaaag aagtgggaga
     6781 acattactgg taaatggtga ttttcagttt tctttaggta aaaaaatcgc gattgtcggt
     6841 gaaaatggtt caggtaagac aaccttatta gaacatatcc gcaaacaagg agaaggaatc
     6901 cttctctctc cgaaagtaag ctttcaagta tatcagcaaa agggttatca aatgacatct
     6961 gaagaatcca tcattcgttt tgtcatgaga caaacagagt tttcagaatc gcttgtccgt
     7021 agtttgctga atcacttagg gtttgctcag gaaactttga cgaaaccgtt atgtacatta
     7081 agtgggggag aagcgacccg tctgacgatt gctttgcttt ttactaagcc aagtaatgtg
     7141 ttgctgttag atgaaccgac taattttatt gatatggcaa cgatcgaagc tttagagaag
     7201 ctaatgcaaa tatatccggg aacgattttg tttacttctc atgattctta ctttgtcgag
     7261 cgtacggctg atgaagttta tgaaataaaa gggcagaaaa taaaaaaagt acttacgaga
     7321 aatttttaaa tgtgctacga tgattttcgt gacaaattta aaggagtggt acagattgcc
     7381 tacaaataaa aaagaaggaa taatttttac aacaatgatg tgttttttga tggtcttggg
     7441 gatgagcgcg tataatttat ggctgcatca agatcttcat ttatcaagtt tgttgattgg
     7501 tctagtgcct ggattcgtgg tggctttcat tttggatgtc ttcatcatcg gtgtgctggc
     7561 gaaaaaaatt gcctttaaac taccagtaaa tccagaaagc aaggtccaaa tgatcttaac
     7621 tatttcatgc ttgatggttt taggaatggt gacatgtatg tctttattcg gtgtgatcat
     7681 ggaacaagga atacctgatc aattattctc tgcttatatg aatgcttgga aaatgaatgt
     7741 gattgccgct ttaccgttgc agttgatcat cgttggcccg ctttcaagaa gtgtgctaaa
     7801 agcaattcaa agtaatgaat gatggaacac tgttatttga aaaaaggcct tggcatgctt
     7861 tttgtcatag tctcttgaat gcagttctag aaaaatagtg atccgaacga taggttttgg
     7921 cctgtcgttc ggatcgcttt ttcttttcca actgtattgc tcttttattg aagataattt
     7981 gctcttcaat agatatttct agaaaggttc tgtttatccc ttactcaaaa aactgctgca
     8041 tttgttctct tacttgctga caccattgga gttcctgctg acaaagcata attctgcgct
     8101 ggatcacaac ataacgtcca aattcttgtt tcttgtctag cgagaggtca ttcaattttt
     8161 tttgattttt ttgcatgatt ttgttcagct gttgttctct agtgaatagc agtccagtag
     8221 ctgttgtttt atcgagcgtc gaaaaagcat agatttttgc tagaaattca tctttttgcg
     8281 taggagcggg agtttcctct ttgatccatt cctctacaag agcacgtcct tcatcagtca
     8341 agtggtagat ctttttttga gagtgctcgt ctttgtccaa aacggtcaca taaccctgct
     8401 ggtctaactt ggcaagcgtt gtatagatct gactgtgatg ggcttcccaa aaaattgtga
     8461 tcagctgctt caattcatac ccagaacgtg gtttggataa aagcacgcat aataatatat
     8521 aggctaaagt attcataaaa atcctacctc tcgtcttatt ttctttatta tatcacagta
     8581 aagtttccac gttttacatg tgcatattga catgtaaaat aaatcatgta tgatgctaat
     8641 agagagaaag ggatggaaat attggagaat caaatagata tacaagaaca aacaaatgaa
     8701 gaaacaaagg ggaaaagttc agcgctagtt gtagcagcta cctgtatgat cgcttttatg
     8761 ggagtgggtc tagtagaccc aatcttaaaa acgattgctg cacagttaaa cgctacgcca
     8821 gctgaaacga ccctgttatt tactagttac atgttagtca ctggtgtggt gatgctattt
     8881 acaggtttct tatctagtcg gatcggtttg aaaaaaacat tgatgacagg attgtttatc
     8941 atcgttgtat tcgctggtct tggcggatta tctgggaata tcggaatgct gattgggtta
     9001 cgtgccggct ggggtgtcgg gaatgcttta tttgtctcta ctgctttagc aacgatcgtc
     9061 agtattttag ttggtaacac agaaaaagca atcatgatgt atgaagcagc acttggctgc
     9121 ggaatggctg ttggccctct agtcggtggg gtcttgggca gtggttcatg gcgttatcca
     9181 ttttttgggg tagcattttt gatgttttgc ggttttatag ccctagcggt acttttacct
     9241 gaaactgaga aaccaaagag aaaagtaggc ctttgggaag gtgtcaaagc gttaagcaat
     9301 cataaacttc gttcagttgg actaatcgca ttgttatata atttcggatt ctttacttta
     9361 cttgcgtatg cgccattttt attgattggc tattctgaat tagaagtcgg ttttgtattt
     9421 tttggctggg gtgtgcttct ggctattgct tcgattttcg ttgcaccggc tcttgagcgg
     9481 aagtttacga cttatcggac aattttagca gcatttgtct tattcattat ttgtttgctg
     9541 ttattaggag ctggaacttc tatgccagtc cttgtaccga tcagcattgt gttagcagga
     9601 tttttccaag gaattatcaa tacactgttg acgacgattg ctatggaaat accaggtctg
     9661 caacgaaacg ttgcttcgtc cagctatagt tttgtccgct ttttcggtgg agctttagca
     9721 ccatttattg caggtgtgat tggtgaacat attggtgaaa gctatgcgtt ttattttgca
     9781 ggtgccattg ttcttatcag tgtatgttgt atcatttatc accgtaatta ttttgctaca
     9841 aatgaagaag aataagaaat cagaggttgt gaaaaagctg tcctgcatca agtcataaga
     9901 a
//
LOCUS       DAFCHQ010000241         9251 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.guided.096, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000241 DAFCHQ010000000
VERSION     DAFCHQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.guided.096"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..794
                     /locus_tag="LNC71_003009"
     CDS             <1..794
                     /locus_tag="LNC71_003009"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBK6467978.1"
                     /translation="SSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASL
                     EGYTEGNPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDT
                     TLAFLDKQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQIT
                     GLEKKIEQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLS
                     DFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1052..2554
                     /gene="eat(A)"
                     /locus_tag="LNC71_003010"
     CDS             1052..2554
                     /gene="eat(A)"
                     /locus_tag="LNC71_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBK6467979.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2582..3148
                     /locus_tag="LNC71_003011"
     CDS             2582..3148
                     /locus_tag="LNC71_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBK6467980.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            3359..4420
                     /locus_tag="LNC71_003012"
     CDS             3359..4420
                     /locus_tag="LNC71_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBK6467981.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4423..5319
                     /gene="murQ"
                     /locus_tag="LNC71_003013"
     CDS             4423..5319
                     /gene="murQ"
                     /locus_tag="LNC71_003013"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBK6467982.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5333..7315
                     /locus_tag="LNC71_003014"
     CDS             5333..7315
                     /locus_tag="LNC71_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBK6467983.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7380..7826)
                     /gene="nrdI"
                     /locus_tag="LNC71_003015"
     CDS             complement(7380..7826)
                     /gene="nrdI"
                     /locus_tag="LNC71_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBK6467984.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            8037..>9251
                     /locus_tag="LNC71_003016"
     CDS             8037..>9251
                     /locus_tag="LNC71_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBK6467985.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAF"
BASE COUNT         2977 a         1604 c         2085 g         2585 t
ORIGIN      
        1 cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa
       61 ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct
      121 tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc
      181 attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta
      241 ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
      301 tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag
      361 atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg
      421 gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca
      481 ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg
      541 atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc
      601 gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt
      661 ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac
      721 acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca
      781 tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga
      841 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
      901 gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga
      961 tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata
     1021 ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac
     1081 attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac
     1141 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
     1201 tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt
     1261 cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga
     1321 ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt
     1381 atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt
     1441 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     1501 tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag
     1561 ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca
     1621 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     1681 atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc
     1741 agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg
     1801 attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg
     1861 gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga
     1921 aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca
     1981 ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga
     2041 gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc
     2101 aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga
     2161 aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg
     2221 aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa
     2281 cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat
     2341 gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac
     2401 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     2461 cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt
     2521 atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg
     2581 tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca
     2641 cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac
     2701 tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc
     2761 aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc
     2821 tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga
     2881 ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt
     2941 acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa
     3001 agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag
     3061 agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga
     3121 taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt
     3181 caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata
     3241 taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt
     3301 ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga gggataatat
     3361 gtttggcttt tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg
     3421 ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga
     3481 tgtttcggca tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact
     3541 agaattgatg gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga
     3601 tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa
     3661 tcagcagatt gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac
     3721 gcctaaagat atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg
     3781 gcataattat tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa
     3841 tcaatggctc aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt
     3901 gagaggccca ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct
     3961 tgcaggcgct ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg
     4021 tctgtcagaa aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct
     4081 ccatacggaa cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg
     4141 gcaagacgat gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc
     4201 tatcccagca agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa
     4261 gtatttgcgc tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa
     4321 agtgaatgta gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc
     4381 tggtatgaac tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta
     4441 gagaacctaa caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt
     4501 tctcaagccg ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa
     4561 gaactgacaa gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga
     4621 cgtctgattt atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa
     4681 tgcgtaccga cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa
     4741 gaagcaatga ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat
     4801 ctaatcaagc tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt
     4861 acaccatatg tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg
     4921 atttcttgca ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat
     4981 gcaggtccag aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt
     5041 atcttgaata tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg
     5101 atggtcgatg tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg
     5161 caagcgacag aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat
     5221 gttaagctgg ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa
     5281 ttagctgaag caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa
     5341 aaacgaactg actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg
     5401 caatgtagaa agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga
     5461 tcttgtaaat gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga
     5521 acagcttcaa gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga
     5581 tttggcaggc gtgaaattag gagaaaaact ttcttctgta agtacagcta cagcaaattc
     5641 atcagcatct ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa
     5701 atcgaaacaa aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt
     5761 gccaatgatt cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc
     5821 aaacttagtc acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat
     5881 gaacatcttg aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc
     5941 acaagttttt ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac
     6001 agggatgaat ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg
     6061 acaaggtggg atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa
     6121 actgcataaa gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcattgat
     6181 tgccatcggt ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg
     6241 catggtcggt gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct
     6301 aggcacatta ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca
     6361 tgtgcaaatg attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg
     6421 tgcaggacaa gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt
     6481 aacagaaatg atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat
     6541 ttacggagta acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat
     6601 cggcggggca gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg
     6661 agccgctttg atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt
     6721 ggcagcttat gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt
     6781 agaaaaagaa gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt
     6841 acctcaaact tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt
     6901 ttctttagat aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt
     6961 tgtagaacca gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt
     7021 cccaacgaaa cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat
     7081 gggaatcaac acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca
     7141 gcaagtgaca cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg
     7201 caaagaaaca tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt
     7261 ggaaaaaaca ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga
     7321 tagaaacaat agaaacgaaa gaagaaactg aaatctattt ttcagcttct tcttttttat
     7381 tatttattgt tttctgctaa aatctgataa attcgtgtca catcagatgg tgttccccgt
     7441 aattcataat ctgccaagaa aggaaagcca aatgtttgtg catattgttt ggctgttaga
     7501 caatactgat tattgaagtt tttatttccg ctaccgacta caccaagaca aagtttatga
     7561 ttgtcttcat gttccagata ttcacgcatc gtttcggtca agatttctgt atcaccgtta
     7621 tctaaaccat ttccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct
     7681 ttcgtaaaat cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct
     7741 tctgcatgca ttttttcagc atattctgct agatgtttgg caaaggcacg cgtatttcct
     7801 gaaatcgata tatataaaat gttcatggat catcctccaa ttctttcctt ttagtatagc
     7861 atctttttta agttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata
     7921 atgagaaaaa agccggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat
     7981 aaaaaaggtt tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg
     8041 atacagagca acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt
     8101 cagcgattgg aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc
     8161 cagctatttt cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca
     8221 ataatatcaa taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag
     8281 gttttggccg tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag
     8341 gcaacgtagc ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg
     8401 aaggtgggca aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat
     8461 ggctagtgaa tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta
     8521 aattagtgcc attattttta tttattgtag tagctatcag cacgttttcg atccaacaat
     8581 tcattgataa cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt
     8641 ccagtcaagt attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag
     8701 cctctgtcgt aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag
     8761 ggatattcgg cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga
     8821 cgatgaatca gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga
     8881 tggttggtaa atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg
     8941 tttggctctc atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac
     9001 ttttgcctaa agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat
     9061 tgacgacgtt atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata
     9121 actttgctta cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt
     9181 accaaatgaa actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag
     9241 caagtgcctt t
//
LOCUS       DAFCHQ010000242         5851 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.guided.125, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000242 DAFCHQ010000000
VERSION     DAFCHQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.guided.125"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            322..1014
                     /locus_tag="LNC71_003017"
     CDS             322..1014
                     /locus_tag="LNC71_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBK6467986.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="LNC71_003018"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="LNC71_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HBK6467987.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="LNC71_003019"
     CDS             complement(1910..3862)
                     /locus_tag="LNC71_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HBK6467988.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4105)
                     /locus_tag="LNC71_003020"
     CDS             complement(3869..4105)
                     /locus_tag="LNC71_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467989.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="LNC71_003021"
     CDS             4507..4797
                     /locus_tag="LNC71_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBK6467990.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            5511..5813
                     /locus_tag="LNC71_003022"
                     /pseudo
     CDS             5511..5813
                     /locus_tag="LNC71_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1997 a          925 c         1010 g         1919 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5821 taaaatattt tgtgtaaatg aaaaaatcca t
//
LOCUS       DAFCHQ010000243         4802 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.guided.136, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000243 DAFCHQ010000000
VERSION     DAFCHQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.guided.136"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            90..785
                     /gene="vanR"
                     /locus_tag="LNC71_003023"
     CDS             90..785
                     /gene="vanR"
                     /locus_tag="LNC71_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBK6467991.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="LNC71_003024"
     CDS             763..1917
                     /locus_tag="LNC71_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBK6467992.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="LNC71_003025"
     CDS             2132..3100
                     /locus_tag="LNC71_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBK6467993.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="LNC71_003026"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="LNC71_003026"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBK6467994.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="LNC71_003027"
     CDS             4130..4738
                     /locus_tag="LNC71_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBK6467995.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DAFCHQ010000244         3732 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.guided.159, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000244 DAFCHQ010000000
VERSION     DAFCHQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.guided.159"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            373..1008
                     /locus_tag="LNC71_003028"
     CDS             373..1008
                     /locus_tag="LNC71_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6467996.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1162..1239
                     /locus_tag="LNC71_003029"
                     /pseudo
     CDS             1162..1239
                     /locus_tag="LNC71_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            1484..1600
                     /locus_tag="LNC71_003030"
     CDS             1484..1600
                     /locus_tag="LNC71_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBK6467997.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            1616..3535
                     /gene="tet(M)"
                     /locus_tag="LNC71_003031"
     CDS             1616..3535
                     /gene="tet(M)"
                     /locus_tag="LNC71_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBK6467998.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            3654..>3732
                     /locus_tag="LNC71_003032"
     CDS             3654..>3732
                     /locus_tag="LNC71_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBK6467999.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYC"
BASE COUNT         1280 a          570 c          762 g         1120 t
ORIGIN      
        1 aatagtgccc ccctgttaga taacgggggc acagacaagg ctcgcaggct cgccagccga
       61 caaagcattt cttccccttg acacaaggtg tcatggcggt aagcctgaga ggcttccgcc
      121 accgtgtcag aaatactttg tcagcaagtt ctgttttgcc ttttctaaca agttccattt
      181 caaccgatat tcatatctac ggatttaatc agataggtat ttctttttga atgtttttat
      241 atttcattct attgtgctta ttcgattaca agaacatagt aattcttata tgcttatttg
      301 tcaatatctt tttttgcctt tacaaatcat attaagcatg atagacttat aatagcgaag
      361 atataacgag atatggaggg ttatgcaatg aaaataagcc atttaggaaa taatatacag
      421 accataagaa aatttagggg aatgaaacaa caggaacttg cggacaaaat cggtatcaat
      481 atgcagagcc tttccaagat tgaaagaggg ttgaactatc ctgcctatga aacgctggaa
      541 aagataatgg aagtgctgga cgttacgccc aatgaattgt tgtcgggaga atggaagtat
      601 gtcaatcaat ctgaaaagga agtctgtcag tttttaagag ccgaagaacg tctaaatgca
      661 gaactgaaac atggacacta tgataacttc tttgacagcg aggaagaatg gctggaatat
      721 gagttagaaa agttacggga atatattaca gactatatca acgggaaaag cattgaagcg
      781 tccgaccttt acccaatcaa agaatttatc cagcatttga agtttcaaaa attattagac
      841 cgctatgatg atttatacag tatggatatg tttggggaaa gcatagaggg acataagtat
      901 aagacacctt atcaagtcgt gaaaatgata aacccgaact caaaagaaga tatggaactg
      961 ttacgagaag tattaaggaa taacaatttt gatgatgagg acgagtaact tttttcctct
     1021 tgcgagaaga tacagcaaac aaaaagcccg atcaagagtg caaagcacca ccaatggtgg
     1081 caaagctctt gacagggctt tttcttttct gttgtgggca atcaagagga agaaagggat
     1141 aaaatctgat ttgtggtaaa tcaggcaaag gcaacgcagg tatctcaatt tgatttggta
     1201 cttgaaaaga acgggagtaa ttggaagatt atagaataac aaatattggt acattattac
     1261 agctattttg taatcacgta ctctctttga taaaaaattg gagattcctt tacaaatatg
     1321 ctcttacgtg ctattattta agtatctatt taaaaggagt taataaatat gcggcaaggt
     1381 attcttaaat aaactgtcaa tttgatagtg ggaacaaata attggatgtc cttttttagg
     1441 agggcttagt tttttgtacc cagtttaaga atacctttat catgtgattc taaagtatcc
     1501 ggagaatatc tgtatgcttt gtatgcctat ggttatgcat aaaaatccca gtgataaaag
     1561 tatttatcac tgggattttt atgccctttt gggtttttga atggaggaaa atcacatgaa
     1621 aattattaat attggagttt tagctcatgt tgatgcagga aaaactacct taacagaaag
     1681 cttattatat aacagtggag cgattacaga attaggaagc gtggacaaag gtacaacgag
     1741 gacggataat acgcttttag aacgtcagag aggaattaca attcagacag gaataacctc
     1801 ttttcagtgg gaaaatacga aggtgaacat catagacacg ccaggacata tggatttctt
     1861 agcagaagta tatcgttcat tatcagtttt agatggggca attctactga tttctgcaaa
     1921 agatggcgta caagcacaaa ctcgtatatt atttcatgca cttaggaaaa tggggattcc
     1981 cacaatcttt tttatcaata agattgacca aaatggaatt gatttatcaa cggtttatca
     2041 ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa cagaaggtag aactgtatcc
     2101 taatatgtgt gtgacgaact ttaccgaatc tgaacaatgg gatacggtaa tagagggaaa
     2161 cgatgacctt ttagagaaat atatgtccgg taaatcatta gaagcattgg aactcgaaca
     2221 agaggaaagc ataagatttc agaattgttc tctgttccct ctttatcatg gaagtgcaaa
     2281 aagtaatata gggattgata accttataga agttattact aataaatttt attcatcaac
     2341 acatcgaggt ccgtctgaac tttgcggaaa tgttttcaaa attgaatata caaaaaaaag
     2401 acaacgtctt gcatatatac gcctttatag tggagtacta catttacgag attcggttag
     2461 agtatcagaa aaagaaaaaa taaaagttac agaaatgtat acttcaataa atggtgaatt
     2521 atgtaagatt gatagagctt attctggaga aattgttatt ttgcaaaatg agtttttgaa
     2581 gttaaatagt gttcttggag atacaaaact attgccacag agaaaaaaga ttgaaaatcc
     2641 gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct gaacagagag aaatgttgct
     2701 tgatgccctt ttggaaatct cagatagtga tccgcttcta cgatattacg tggattctac
     2761 gacacatgaa attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact
     2821 gttgcaagaa aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga
     2881 gagaccgtta aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg
     2941 ggcttccatt ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag
     3001 ctcggtttct cttggatact taaatcaatc gtttcaaaat gcagttatgg aggggatacg
     3061 ctatggctgt gaacaaggat tgtatggttg gaatgtgacg gactgtaaaa tctgttttaa
     3121 gtatggctta tactatagcc ctgttagtac cccagcagat tttcggatgc ttgctcctat
     3181 tgtattggaa caagtcttaa aaaaagctgg aacagaattg ttagagccat atcttagttt
     3241 taaaatttat gcgccacagg aatatctttc acgagcatac aacgatgctc ctaaatattg
     3301 tgcgaacatc gtagacactc aattgaaaaa taatgaggtc attcttagtg gagaaatccc
     3361 tgctcggtgt attcaagaat atcgtagtga tttaactttc tttacaaatg gacgtagtgt
     3421 ttgtttaaca gagttaaaag ggtaccatgt tactaccggt gaacctgttt gccagccccg
     3481 tcgtccaaat agtcggatag ataaagtacg atatatgttc aataaaataa cttagtgtat
     3541 tttatgttgt tatataaata tggtttcttg ttaaataaga tgaaatattt tttaataaag
     3601 atttgaatta aagtgtaaag gaggagatag ttattataaa ctacaagtgg atattgtgtc
     3661 ctgtatgtgg aaataaaaca cgattaaaga taagggaaga tactgaatta aaaaaattcc
     3721 ccctctattg tc
//
LOCUS       DAFCHQ010000245         3278 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.guided.163, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000245 DAFCHQ010000000
VERSION     DAFCHQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.guided.163"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..188
                     /locus_tag="LNC71_003033"
     CDS             <1..188
                     /locus_tag="LNC71_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBK6468000.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="LNC71_003034"
     CDS             203..1072
                     /locus_tag="LNC71_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBK6468001.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="LNC71_003035"
     CDS             1053..1787
                     /locus_tag="LNC71_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBK6468002.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="LNC71_003036"
     CDS             1820..2728
                     /locus_tag="LNC71_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBK6468003.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="LNC71_003037"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="LNC71_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAFCHQ010000246         1148 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.guided.211, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000246 DAFCHQ010000000
VERSION     DAFCHQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.guided.211"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..76
                     /locus_tag="LNC71_003038"
     CDS             11..76
                     /locus_tag="LNC71_003038"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBK6468004.1"
                     /translation="MFYTIIWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="LNC71_003039"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="LNC71_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBK6468005.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
LOCUS       DAFCHQ010000247          997 bp    DNA     linear   BCT 08-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate V1518
            SAMN22631066-rid13289063.guided.218, whole genome shotgun sequence.
ACCESSION   DAFCHQ010000247 DAFCHQ010000000
VERSION     DAFCHQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN22631066
            Sequence Read Archive: SRR16608820
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001171323.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001171323.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2021 05:50:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 31 of 74
            Pseudo Genes (incomplete)         :: 38 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN22631066-rid13289063.guided.218"
                     /isolate="V1518"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2016-07-25"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            281..829
                     /gene="aac(6')"
                     /locus_tag="LNC71_003040"
     CDS             281..829
                     /gene="aac(6')"
                     /locus_tag="LNC71_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBK6468006.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          351 a          145 c          208 g          293 t
ORIGIN      
        1 tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag caaaagataa
       61 tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc ctgatctttc
      121 ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga taaataatga
      181 aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt cgttttttca
      241 aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca gtgaatttga
      301 ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga cttggccgga
      361 agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag aacgaatcgc
      421 ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc ctcaatacgg
      481 tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa agaaccaaat
      541 aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag gaatcacgat
      601 ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg acctgtatga
      661 gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt atgaattcta
      721 tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc
      781 ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat aaaaagaggt
      841 tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt acttcttgaa
      901 tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg tgtaccagag
      961 gttgtgaaaa agtcgcttag tttcgagtag taagaaa
//
